The University of the West Indies St. Augustine Campus COURSE/PROGRAMME APPROVAL PROCESS CHECKLIST Course/Programme Title: Department: Minor in Biology Life Sciences Faculty: APPROVAL/REVIEW RECEIVED APPROVAL PROCESS Yes Stakeholders/ External Review: Faculty Curriculum Review Committee/ Other Intra-Campus Review Library AQAC Meeting Date: No COMMENTS RECEIVED Science and Technology ACTION TAKEN Date Approved/ Review Received x x No additional library resources required CETL x Courses associated with the proposed revision to the Minor in Biology were approved by the Centre for Excellence in Teaching and Learning during the 105-2016 Academic Year. And as such, the proposed revision to the Minor in Biology has been reviewed and approved. Cross-Campus Reviews: x 25/02/2016 Cave Hill The proposal is straightforward and welljustified in your document. You have our full support. None necessary SECRETARIAT VERIFICATION DATE - The proposal was circulated among staff. Mona x 24/02/2016 - Overall, there were no major queries/concerns. It is a coherent programme of study and training in the biological sciences. None necessary - The programme provides exposure to the fundamental principles of biology, including studies of the structure, organization, and diversity of life. - It was noted that the layout of the credits on page 2 could be more clearly explained, as initially it seems that the total advanced credits for the minor is 24. Open Campus Boards/Committees: Faculty Board AQAC x NB: Items listed in no particular order Verified By: Faculty/Department: Approved By: Academic Board Date: Office of the Campus Registrar, St. Augustine, November 20, 2013 Secretariat: CF&GPC BUS UF&GPC BANNER SAS ACTT The University of the West Indies Faculty of Science and Technology – St. Augustine Campus Department of Life Sciences (DLS) Proposal for Revision of the Minor in Biology January 2016 1 Introduction: Present requirements and philosophy of the DLS Biology Minor. The present structure of the Minor in Biology is based on a philosophy that offers non-biology majors an understanding of the organism at each organizational level (sub-organismal, organismal and whole systems/environment interactions). This is reflected in the course selection for the minor which includes courses that examine the biodiversity of plants and animals, biochemistry, genetics, evolutionary biology and functional design in biology. The current course requirements for the DLS Biology Minor are stated below: Level I courses: BIOL1262 (Living Organisms I) BIOL1263 (Living Organisms II) BIOL1362 (Biochemistry I) BIOL1364 (Genetics I) Additionally, students must complete 15 advanced level credits (5 courses) inclusive of the two 3-credit core courses listed below: Level II- III CORE courses: BIOL2262 (Evolutionary Biology) BIOL3164 (Function and Design in Biology) Students must ALSO select three (3) additional courses (9 credits) taken from the following list of optional courses: Level II- III OPTIONAL courses: BIOL2165 (Genetics II) BIOL2360 (Biochemistry II) BIOL3770 (Plant Pathogens) BIOL2464 (Fundamentals of Ecology) BIOL2764 (Physiology of Plants) BIOL2867 (Physiology of Animals) 2 The pre-requisites for the core course BIOL3164 includes BIOL3773, which is not an optional course in the minor, in addition to BIOL2764 AND BIOL2867. Therefore to complete the minor in Biology, the student must complete at least 18 advanced credits as follows: - BIOL2262 - Evolutionary Biology BIOL2764 – Physiology of Plants BIOL2867 – Physiology of Animals BIOL3164 - Function and Design in Biology One Elective from BIOL2165 - Genetics II, BIOL2360 - Biochemistry II, BIOL3770Plant Pathogens, BIOL2464 - Fundamentals of Ecology BIOL3773 – Plant Anatomy (not part of the minor) This current structure requires students to complete more than the normal 15 credits required for the biology minor. Additionally, the structure does not offer any choice or flexibility to students choosing the Biology Minor. In contrast to majors, minors are generally designed to give students a breadth of knowledge on a topic outside of their major degree. Therefore while the Major focuses on mastering a particular field, the Minor should be about exploration of a new topic. Proposed Structure of the new DLS Biology Minor. The Biosciences Subject Benchmark Statement document section 5.2.1(i), page 13, stresses that “a common element to all biosciences degree programs is the need to facilitate interdisciplinary and multidisciplinary approaches in advancing knowledge and understanding of the processes and mechanisms of life”. This idea can be supported and encouraged by allowing a scope in our Biology Minors that attracts students from other science/ non-science disciplines to explore content in biology. The student should thus be offered some flexibility in choosing what they are interested to explore and should be allowed a course selection that supports their major from an interdisciplinary perspective. Biology programs in a few top-ranking universities in the USA, Canada and Australia were reviewed. These higher education institutions, namely Stanford University, The University of Toronto, MIT, Rutgers, Boston University and the Australian National University, collectively highlighted six core areas common in a typical Biology Minor (Appendix 1) as defined by their respective offerings. These areas include Cell & Molecular Biology*, Genetics, Biodiversity, Evolution/Ecology and Biochemistry. Of these core areas, Genetics, Biodiversity and Biochemistry are presently addressed at Level I of the Biology Minor. To cover the remaining core areas as suggested by the composition of the typical Biology Minor, students should be required to complete an Evolutionary biology course and a Cell Biology course. *The Molecular Biology in this context examines structure and form of biological molecules, topics that are covered in DLS offerings of Biochemistry I and Genetics I. 3 The proposed requirements for the DLS Biology Minor as per the justification stated above are as follows: Level I CORE courses: BIOL1262 (Living Organisms I) BIOL1263 (Living Organisms II) BIOL1362 (Biochemistry I) BIOL1364 (Genetics I) Additionally, students must complete 15 advanced level credits (5 courses) inclusive of the two 3-credit core courses listed below: Level II CORE courses: BIOL2061 (Cell and Developmental Biology) BIOL2262 (Evolutionary Biology) Students must ALSO select three (3) additional Level II-III Biology courses (total of 9 credits) from the list below. Students may choose freely from the list of Level II Biology courses. However, students wishing to pursue any Level III Biology courses must first seek departmental advising on prerequisites, course availability, timetabling and other academic guidance. List of Level II Biology courses BIOL 2163- Biostatistics BIOL 2164- Principles of Molecular Biology BIOL 2165- Genetics II BIOL 2265- Fundamentals of Microbiology BIOL 2360- Biochemistry IIA BIOL 2464- Fundamentals of Ecology BIOL 2764- Physiology of Plants BIOL 2867- Physiology of Animals 4 List of Level III Biology courses BIOL 3063- Marine Ecology and Oceanography BIOL 3162- Principles of Microbial Biotechnology BIOL 3164- Function & Design in Biology BIOL 3263- Introduction to Bioinformatics BIOL 3363- Medical Biotechnology BIOL 3366- Plant Biotechnology and Genetic Engineering BIOL 3409- Caribbean Coral Reefs BIOL 3462- The Ecology of Freshwaters BIOL 3465- Tropical Forest Ecology and Use BIOL 3466- Coastal Ecosystems & Resource Management BIOL 3468- Biodiversity and Conservation BIOL 3768- Plant Diversity & Systematics BIOL 3769- Plant Genetic Improvement BIOL 3770- Plant Pathogens BIOL 3771- Environmental Plant Physiology BIOL 3772- Plant Development BIOL 3773- Plant Anatomy BIOL 3866- Parasite Biology BIOL 3867- Biology of Animal Behaviour BIOL 3868- The Ecology of Humans BIOL 3870- Insect Biology BIOL 3960- Environmental Microbiology BIOL 3961- Principles of Medical Microbiology BIOL 3970- Aquaculture BIOL 3971- Fisheries Management BIOL 3XXX - Caribbean Island Ecology and Biogeography See Appendix II for course descriptions. Note that assessments and grading schemes for several course outlines have since been amended to the new UWI grading scheme. Please note all courses were approved by CELT in Semester II 2012/2013. 5 Resources Needed: The courses that comprise the newly proposed Minor in Biology are all pre-existing courses that were revised in the recent DLS departmental-wide change from 4-credit to 3 credit course weightings. As such, no new resources are needed to facilitate the implementation of this new proposed structure. There are no new courses for this proposed Biology Minor. Summary: The proposed structure of the new Biology Minor in the DLS resolves previous issues related to credit loadings but maintains adherence to the philosophical underpinnings by providing students with an understanding of biology at the sub-organismal, organismal and whole systems levels. The modifications proposed in the structure of the new minor, not only benchmarks our minor to international standards, but also allows non-biology major students to explore, with some measure of flexibility, courses with greater relevance to their major degree program. 6 Appendix I: Examination of Biology Minor offerings at a few Top-Ranked International Universities University Name Summary of Requirements for minor Details of Requirements for minor The University Students interested in biology BIO 120H1 – Adaptation and Biodiversity of Toronto must take 2 Level I courses + ANY 2 and BIO 130H1 – Molecular and Cell Biology. from the list of core courses EEB BIO220H1; Genomes to Ecosystems CSB BIO230H1 – Genes to Organisms; 38 Programs EEB BIO251H1; Form, function and plant dev. in Life Sci. CSB BIO270H1; BIO271H1 (Animal Phys I and II); Rank: Top 16 CSB BIO260H1 (Concepts in Genetics) Stanford 6 courses including two courses 2 courses from the Bio Core University from the Bio Core Lecture series (BIO 41, 42, 43, BIOHOPK 43) must be taken, BIO 41: Genetics, Biochemistry, and Mol Biology BIO 42: Cell Biology and Animal Physiology BIO 43: Plant Biology, Evolution, and Ecology Rank : Top 5 MIT BIOHOPK 43: Plant Biology, Evolution, and Ecology Core: organic chemistry, genetics and List of 22 courses - range of biology sub-disciplines general biochemistry Total of 60 units for the Biology minor Each course = 12 units Rank : Top 5 also select any 2 from the list Australian This biology minor= 24 units Evolution, Ecology and Genetics National Any two core courses (6 credits each) Molecular and Cell Biology University Plus minimum two 6-credit courses Human Biology Rank: 19th from ANY 2000-3000 level BIOL courses Diversity of Life Boston Five 4-credit courses. (3 Bio courses) Cell and Molecular Biology University The 2 core courses in biology Neurobiology and behavior cover the topics: Ecology and Evolution Rank: 37th cell and molecular biology neurobiology and behavior, and ecology and evolution. Core areas identified include Cell & Molecular Biology, Evolution, Ecology, Genetics, Biodiversity, Biochemistry. 7 APPENDIX II- DESCRIPTION OF CORE AND ELECTIVE COURSES IN BIOLOGY MINOR THE UNIVERSITY OF THE WEST INDIES ST. AUGUSTINE FACULTY OF SCIENCE AND AGRICULTURE DEPARTMENT OF LIFE SCIENCES Course Description Title: BIOL 1262 Living Organisms 1 Credits: 3 Level: Undergraduate –Year 1 Semester: 1 Offering Department: Department of Life Sciences, Faculty of Science and Agriculture, The University of the West Indies, St. Augustine Course coverage: Origin and evolution of living organisms, prokaryotes, autotrophic protists, non vascular and vascular plants. Pre-requisites: CAPE Biology (Units I and II) or OR Preliminary Biology I and II (BIOL0061 and BIOL0062) OR equivalent. Significance/ Rationale: Prokaryotic organisms have been present on earth for almost four billion years. These early bacteria evolved diverse physical and metabolic characteristics that contributed to creating conditions necessary for the emergence and sustenance of higher forms of life. Photosynthetic bacteria gave rise to algae, from which plants evolved. As primary producers, plants form the most important foundation for the maintenance of heterotrophic organisms on earth. Plant evolution entailed a number of strategies to escape the aquatic environment and survive the new challenges imposed by the need for support, acquisition of nutrients, and reproduction on land. Some of the first plants were more successful than others, notably the ferns and conifers. Higher plants remain rooted to one spot throughout their lives, yet with the simple structural elements of root, stem, leaf, they have evolved into some 235,000 species, ranging enormously in size, occupying many diverse and challenging habitats, with plants of some species surviving for thousands of years. The greatest success came with the evolution of the flower, and flowering plants account for all but about 33,000 species of land plants. 1 Enrolment Capacity: 400 students (maximum) Purpose of Course This course is designed primarily for 1st year students registered for the Biology degree option in the Department of Life Sciences. The course provides an introduction to the theories on the origin of life on earth and the diversity of autotrophic protists and plants. The characteristics, range of structure, reproduction, life cycles and habitats of selected protists and plants are covered. Laboratory exercises will provide an opportunity for ‘hands on’ experience with representative members of various groups’ in particular local species. Letter to Student Welcome to this Level 1 Introductory course on Living Organisms. The aim of this handout is to provide you with an outline of the course content as well as related information with respect to course objectives and methods of assessment. This 3 credit course will be taught through a series of lectures (18 hours), practicals (12 two-hour sessions) and tutorials (6 hours). Attendance at all sessions will be absolutely necessary if you hope to do well in the course. Instructor Information BIOL1262 will be managed by a course coordinator and would be taught be a team of experienced biology lecturers, supported by a Teaching Assistant/Instructor and laboratory demonstrators. Office hours for the coordinator and lecturers are posted on the faculty bulletin boards and are available from the Department of Life Sciences’ general office. Team members can also be contacted via e-mail. Dr. Judy Rouse-Miller (Course Coordinator) Natural Sciences Building (Old Wing) Ext. 83089 [email protected] XXXXXX (TA) Content BIOL1262 covers the following content: Evolutionary Concepts Archaebacteria & Eubacteria Autotrophic protists; classification, characteristics, identification Phylogeny and classification of plants Physical features, challenges and adaptations of plants in aquatic and terrestrial environments; The plant life cycle – alternation of generation Bryophytes 2 Seedless vascular plants Seed plants – Gymnosperms Seed plants – Angiosperms (form and function) Photosynthetic systems Reproductive systems Ecology of plants Goal The goal of BIOL1262 is to provide students with a foundation on the concepts of origin of the prokaryotes and the evolution and diversity of photosynthetic organisms, as a prerequisite for advanced biology courses in the Department of Life Sciences. General Objectives 1. Introduce students to the major groups of prokaryotes, autotrophic protists and plants, their evolutionary associations, and adaptive radiation 2. Develop skills appropriate to the study of plants and prokaryotes in the laboratory Learning Objectives Upon successful completion of this course, students should be able to: 1. Outline the main theories on the origin of life on earth 2. Describe the characteristic features of selected prokaryotes 3. Compare the biology of autotrophic protists and plants. 4. Classify and identify common plants that occur in the Caribbean using the Linnaean system 5. Arrange taxonomic and non – taxonomic terms of formal and informal classification schemes to show the relationship between the terms. 6. Explain the functional consequences of different types of body and tissue organization in plants 7. Outline general trends in plant evolution 8. Identify the sequence of events/processes and the structures of the generalised plant life cycle 9. Outline the life cycle of the major plant groups (Bryophytes, Pteridophytes, Gymnosperms and Angiosperms) 10. Outline the main associations between the major taxonomic groups of plants 11. Describe the adaptive radiation of the major groups of plants 12. Construct a dichotomous key based on observable morphological characteristics which could be used to key out a group of plants from terrestrial and aquatic environments 13. Describe the challenges to existence faced by the early aquatic colonizers of land 14. Solve simple problems in plant science 15. Demonstrate laboratory skills appropriate to the study and interpretation of living and preserved botanical specimens 3 Mode of Delivery: Lectures -18 hours: Didactic; interactive Tutorials -6 hours: Interactive; mind maps; problem-solving Laboratory classes- 24 hours (12 two- hour sessions): Interactive practical tasks; problem-solving Assessment: Final Examination: 50% (One 2-hour Comprehensive paper) Course Work: 50% - Writing across the curriculum; critical thinking exercises - Laboratory reports (10 x 2% ) - One in-course test - Tutorial Attendance and participation 5% 20% 20% 5% ASSIGNMENTS: Coursework in BIOL1262 will be assessed in the following ways: 1. Writing across the curriculum- critical thinking exercises-5%. These activities are designed to encourage students to become active learners by engaging knowledge to further develop understanding. The exercises would help students understand content and express ideas. A total of five (5) simple exercises out of eight (8) worth a total of 5 marks (1 mark per exercise) would be given during lectures or tutorials (no more than one per week) without advanced notice. Each exercise would take between 5 – 10 minutes to complete and would comprise of varying activities including: Focused free-writing: Students are asked to write briefly on their knowledge of a topic; identifying misconceptions from previous knowledge and new information learnt. Glossary: Students are asked to prepare a glossary 5 terms which they met for the first time in the previous lecture. Complete the life cycle: Students are asked to complete the life cycle diagram given to demonstrate their knowledge of the sequence of events and structures which occur in the plant life cycle Construction of dichotomous key: Students are asked to construct a dichotomous key to which can be used to key out (identify) a group of specimens. Concept map: Students are asked to prepare a concept map which shows the relationship between kingdom, division, major plant group terms, and common names used throughout the lecture. 4 Group presentation- The tutorial class would be divided into groups and each group would be required to design the sporophyte generation of a plant, which based on its morphological features would be placed into one of the plant divisions studied – the presentation should include a diagram which is 1-5x the actual size of the plant. Reflective statement: students should write a reflective statement on one of the labs completed: students should identify problems encountered, techniques mastered or improved, what was done well and why and what was learnt and what is unclear. 2. In-course Test-20% One in-course test would be given worth 20%. The test would be given in week 12 of the semester and would comprise of MCQs and structured questions. 3. Laboratory reports-20% Students would be required to submit 12 lab reports/completed worksheets for practical exercises. Each report/completed worksheet would be worth 2 marks. 4. Class participation- 5% Student participation in tutorial sessions will be monitored by the tutor and a grade assigned at the end of the semester. Participation would include more than just showing up at tutorial – some evidence of an active role in the tutorial discussion would be required to obtain the full 5% for participation. At the beginning of the semester, the students in each tutorial would engage in developing the exact assessment criteria for this assignment, and the student-developed criteria will be used to determine each student’s participation grade. Cheating, including plagiarism, would not be tolerated. Incidents of cheating would be dealt with according to UWI’s rules and regulations including: - Examination Regulations for First Degrees, Associate Degrees, Diplomas and Certificates. Section (B) Cheating. Regulations 96–102; http://sta.uwi.edu/resources/documents/Exam_and_GPA_regulations.pdf. - University Regulations on Plagiarism (First Degrees, Diplomas and Certificates). http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf. 5 EVALUATION: BIOL1262 will be evaluated in two ways – (a) through the offices of the Class Representative and the Life Sciences Student-Staff Liaison Committee, and (b) an end of semester course evaluation survey. The class will elect four class representatives (one per lab stream), whose role is to act as a mediator between the Life Sciences academic staff and the students in the class. The representatives will attend Liaison Committee meetings (held at least twice per semester), where they will present feedback on the course to the Department for action. The UWI performs a course evaluation survey at the end of every semester, and this information will also be used for overall assessment of the course and guide possible actions for improvement in subsequent semesters. TEACHING STRATEGIES: A combination of teaching strategies will be adopted in BIOL1262. The primary teaching strategy will be based on the face to face classroom lectures and discussions. Videos of lecture presentations would be made available to students prior to the lecture session via podcast media. Students are advised to view these videos before the lecture so that more emphasis can be placed on discussions and answering questions, thereby facilitating deep learning. This would also afford the time to have assessment exercises on “writing across the curriculum”. MyeLearning will be utilized throughout the course as a means to provide access to course materials such as Powerpoint presentation files, animations, weblinks, lessons and quizzes. This medium would also be used as a portal for student-lecturer communications and the dissemination of coursework feedback. A total of six tutorial sessions would be given for the semester. These would be small group sessions which would be conducted by instructors or tutors, who would normally by senior postgraduate students in Life Sciences. Students must attend tutorial sessions. Tutorials sheets will be assigned prior to each session via myeLearning, and students are expected to attempt the solutions before coming to tutorial. The goal of the tutorial session is to give students a more hands-on experience with the course material and easier access to course instructors. Students will be expected to ask and answer questions on material that is unclear, propose solutions to questions on the tutorial sheet and to generally participate fully in the tutorial activities. Tutors will not merely be going through the answers to the tutorial questions in these sessions. Practical sessions would include demonstrations and problem solving exercises to develop practical skills and enhance understanding of content covered in the theory section. These exercises would include analysis of specimens, experiments and use of identification keys to assign organisms into different levels of taxa In order to pass the course, you must gain an overall passing mark of 40%. Any student who misses more than 25% of practical classes or tutorial sessions without a medical or other valid excuse can be debarred from writing the final exam. 6 Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 Texts Essential Texts 1. Peter Raven, Ray Evert, and Susan Eichhorn 2005. Plant Biology 7th Edition, WH Freeman Publishers. 2. Bidlack, James and Shelley Jansky. (2010) Stern's Introductory Plant Biology, 12th Ed. McGraw-Hill Science/Engineering/Math Recommended reading: Hickey, M. and King, C.2000. The Cambridge Illustrated Glossary of Botanical Terms Kingsley R. Stern, Shelley Jansky, James Bidlack (2007) Introductory Plant Biology, 11th Ed. McGraw-Hill Companies. 7 James D. Mauseth (2008) Botany: An Introduction to Plant Biology, 4th Ed. Jones & Bartlett Publishers. Ennos and R and E. Sheffield 2000. Plant Life, Blackwell Science, USA. Joanne Willey, Linda Sherwood and Chris Woolverton (2007) Prescott, Harley and Klein’s Microbiology (7th edition), McGraw-Hill Handouts 1. Classification handout 2. Dichotomous keys 3. Alternation of generation life cycle 4. Guidelines for making Biological Drawings 5. Guidelines for preparing the lab report 6. Technical terms Online resources 1. 2. 3. 4. UCMP Glossary Life History http://www.ucmp.berkeley.edu/glossary/gloss6lifehist.html UCMP Glossary http://www.ucmp.berkeley.edu/glossary/gloss8botany.html Classification: http://www.botany.hawaii.edu/faculty/wong/BOT135/Lect04_c.htm The National Herbarium http://sta.uwi.edu/herbarium/ Readings 1. 2. 3. 4. 5. 6. 7. 8. 9. Botany, An Introduction to Plant Biology by James Mauseth 2nd ed. Chapter 1 Introduction to Plants and Botany pp 1-13 Chapter 4 Growth and Division of the Cell pp 84-85 Chapter 18 Classification and Systematics pp 492-494, 507-509 Chapter 21 Algae pp 575 -578, 591-593 Chapter 22 Non- vascular plants pp 611-614 Chapter 23Vascular plants without seed pp 636-639 Chapter 24 Seed Plants I: Gymnosperms pp 672-675 Chapter 25 Seed Plants II: Angiosperms pp 703-70 8 Course Calendar LECTURE TOPICS (by week) Week Practical General Biology 1 2 Lecture/Tutorial Laboratory Techniques Lecture 1. Introduction; The scientific inquiry process. Evolution explains unity and diversity of life ; Levels of organization in biology: Structure and function are correlated in all levels of organization in biology Lecture 2. Origin of Life –Theories Isolation and observation of prokaryotes Lecture 3. Diversity of contemporary life on earth: the origin of prokaryotes, evolution of the eukaryotic cell, the genesis of multicellular life, and the adaptive radiations of plants and animals Tutorial 1 Glossary: Students are asked to prepare a glossary 5 terms which they met for the first time in the previous lecture. 3 Prokaryotes 4 Autotrophic protists Chlorophyta, Phaeophyta and Rhodophyta Lecture 4: Structure and function of prokaryotic cells, Diversity of prokaryotes Lecture 5 Dichotomous keys Lecture 6. Plant - like Protists – Algae - Chlorophyta, Phaeophyta and Rhodophyta – characteristics adaptation to habitat Tutorial 2.Construction of dichotomous key: Students are asked to construct a dichotomous key to which can be used to key out (identify) a group of specimens. 5 Non-vascular plants, Bryophytes-liverworts, Lecture 7.Characteristics of Plants Classification (Hierarchal classification) Concept map: Students are asked to prepare a concept map which shows the relationship between kingdom, division, major plant group terms, and common names used throughout the lecture. Lecture 8. Alternation of generation Life cycle – reproduction and variability 6 Non-vascular plants, Bryophytes-mosses Lecture 9. Characteristics of Bryophytes – adaptations to environment – life cycle, reproductive strategy Tutorial 3 Complete the life cycle: Students are asked to complete the life cycle diagram given to demonstrate their knowledge of the sequence of events and structures which occur in the plant life cycle 7 Vascular non seed plants - Pteridophytes: lycopods, ferns Lecture 10. Physical characteristics of the aquatic and terrestrial environments, requirements for plant survival, challenges to existence faced by the early aquatic colonisers of land The dichotomous key and identification of plants Lecture 11. Characteristics of the Microphyllophyta, adaptations to environment, life cycle, reproductive strategy, ecology 9 Focused free-writing: Students are asked to write briefly on their knowledge of a topic; identifying misconceptions from previous knowledge and new information learnt. 8 Vascular non seed plants - Pteridophytes: ferns The dichotomous key and identification of plants 9 Vascular seed plants – Gymnosperms – cycads and pines Lecture 12. Characteristics of the Pteridophyta, adaptations to environment, life cycle, reproductive strategy, ecology Tutorial 4. Complete the life cycle: Students are asked to complete the life cycle diagram given to demonstrate their knowledge of the sequence of events and structures which occur in the plant life cycle Lecture 13. Gymnosperms characteristic features –morphological characteristics, adaptations, life cycle, reproductive strategy , ecology Lecture 14. Gymnosperms characteristic features–life cycle, reproductive strategy, ecology 10 11 12 13 Angiosperms –, seed germination, flower structure, monocot and dicot characteristics Angiosperm families: Virtual Herbarium, Campus field exercise: Identification of plants Review- Lab based Identification of unknown to level of division in the urban habitat Lecture 15. Angiosperms - characteristic features, organ: flowers, stem, leaf, root – structure and function –photosynthesis Tutorial 5. Reflective statement: students should write a reflective statement on one of the labs completed: students should identify problems encountered, techniques mastered or improved, what was done well and why and what was learnt and what is unclear. Lecture 16. Angiosperms life cycle, reproduction Lecture 17 Evolution of the seed habit, Angiosperm seed structure Lecture 18. Concept of the ideal land plant, ecology Tutorial 6. Group presentation- The tutorial class would be divided into groups and each group would be required to design the sporophyte generation of a plant, which based on its morphological features would be placed into one of the plant divisions studied – the presentation should include a diagram which is 1-5x the actual size of the plant. Review 10 THE UNIVERSITY OF THE WEST INDIES ST. AUGUSTINE FACULTY OF SCIENCE AND AGRICULTURE DEPARTMENT OF LIFE SCIENCES Course Description Title: BIOL1263- Living Organisms II Credits: 3 Level: Undergraduate –Year 1 Semester: 1 Offering Department: Department of Life Sciences, Faculty of Science and Agriculture, The University of the West Indies, St. Augustine Course coverage: Diversity of animals: Protozoa, Porifera; Cnidaria, Platyhelminthes, Nematoda, Annelida, Arthropoda, Mollusca, Echinodermata, Chordata; Major fungal & fungal-like organisms including Chytridiomycetes, Zygomycetes, Ascomycetes, Basidiomycetes, Oomycetes, Hyphochytriomycota, Plasmodiophorids & Labyrinthulids Pre-requisites: CAPE Biology (Units I and II) or OR Preliminary Biology I and II (BIOL0061 and BIOL0062) OR equivalent. Significance/ Rationale: Animals are very diverse with more than 1.5 million species described. These represent vast levels of genetic and ecological diversity. When a person thinks of an animal they probably think of a vertebrate, one having a backbone. However, vertebrates account for only about 50,000 species; the rest are invertebrates, animals without backbones. Regardless, all animals share certain characteristics: they are multicellular, heterotrophic, require oxygen for aerobic respiration, reproduce-either sexually or asexually, go through a period of embryonic development, and can move during at least part of their life. Fungi are also a very large, diverse group of eukaryotic organisms with ~90,000 species described out of an estimated 1.5 million species that are thought to exist on earth. These organisms have critical roles in maintaining the environment for sustaining higher forms of life and are utilized in many processes related to medicine, agriculture and industries. However, some species are also causative agents for important human, animal and plant diseases. 11 Enrolment Capacity: 400 students (maximum) Purpose of Course The purpose of this course is to introduce students to this amazing diversity of animal and fungal life through lectures which highlight evolutionary innovations, unique characteristics, as well as features shared by other groups. Laboratory exercises will provide an opportunity for ‘hands on’ experience with representative members of various groups’ in particular local species. Tutorials are small group sessions for students to discuss key topics and related issues. Letter to Student Welcome to this Level 1 Introductory course on Living Organisms. The aim of this handout is to provide you with an outline of the course content as well as related information with respect to course objectives and methods of assessment. This 3 credit course will be taught through a series of lectures (18 hours), practicals (8 three-hour sessions) and tutorials (6 hours). Attendance at all sessions will be absolutely necessary if you hope to do well in the course. Instructor Information BIOL1263 will be managed by a course coordinator and would be taught be a team of experienced biology lecturers, supported by a Teaching Assistant/Instructor and laboratory demonstrators. Office hours for the coordinator and lecturers are posted on the faculty bulletin boards and are available from the Department of Life Sciences’ general office. Team members can also be contacted via e-mail. Prof. Dave Chadee (Course Coordinator) Natural Sciences Building (New Wing) Ext. 83740 [email protected] Dr. Adesh Ramsubhag (lecturer) Rm. 220 Natural sciences Building (Old wing) Ext. 83086 [email protected] XXXXXX (TA) Content BIOL1263 covers the following content: A survey of the phylogeny and characteristics of major invertebrate and vertebrate animal groups. Special topics include 12 1. 2. 3. 4. 5. 6. 7. theories of the origin of unicellularity and multicellularity cell and tissue levels of organization origins and functional significance of body cavities early embryonic development metamerism and tagmosis (tagmatization) colonization of land by arthropods and vertebrates evolutionary trends in vertebrates A survey of the phylogeny and characteristics of major groups of fungi: 1. 2. 3. 4. 5. 6. Introduction to Mycology and importance of fungi to humans and the environment. Chytridiomycota Zygomycetes: Mucorales, Endogonales and Entomophthorales) & Trichomycetes Ascomycetes (and Deuteromycetes) Basidiomycetes Fungal-like organisms: Hyphochytriomycota, Plasmodiophorids & Labyrinthulids Oomycetes Goal The goal of BIOL1263 is to provide students with a foundation in introductory diversity of animals and fungi, as a prerequisite for advanced biology courses in the Department of Life Sciences. General Objectives 1. Introduce students to the major groups of: a. animals, their evolutionary associations, and adaptive radiation; and b. fungi as decomposers, symbionts, and pathogens 2. Develop practical skills appropriate to the study of animals and fungi in the laboratory Learning Objectives Upon successful completion of this course, students should be able to: 1. Describe the characteristic features of major groups of animals and fungi; 2. Classify common animals and fungi using the Linnaean system; 3. Explain the functional consequences of different types of body organization of animals; this includes such factors as level of complexity (single-cell, tissue, organsystem), type of symmetry, whether the body is wormlike, and whether the body is substantially modified (e.g. for a parasitic way of life); 4. Categorize the major groups of animals based on neo-Darwinian evolutionary principles 5. Explain the adaptive radiation of the major groups of animals and fungi 6. Solve simple problems in zoology 7. Compare the roles of fungi as primary decomposers, symbionts, and pathogens 13 8. Demonstrate laboratory skills appropriate to the study and interpretation of living and preserved specimens of animals and fungi Mode of Delivery: Lectures -18 hours: Didactic; interactive Tutorials -6 hours: Interactive; mind maps; problem-solving Laboratory classes- 24 hours (8 three-hour sessions): Interactive practical tasks; problem-solving Assessment: Final Examination: 50% (One 2-hour Comprehensive paper ) Course Work: 50% - Writing across the curriculum exercises: - Laboratory reports (10 x 2% ): - One in-course test; - Tutorial Attendance and participation: 5% 20% 20% 5% ASSIGNMENTS: Coursework in BIOL1263 will be assessed in the following ways: 5. Writing across curriculum exercises-5%. These activities are designed to encourage students to become active learners by engaging knowledge to further develop understanding. The exercises would help students understanding content and expressing ideas. A total of five simple exercises worth a total of 5 marks (1 mark per exercise) would be given during lectures or tutorials (no more than one per week). Each exercise would take between 5 – 10 minutes to complete and would comprise of varying activities including: a. Focused free-writing- Students may be asked to briefly write on their understanding or perspective on a subject/topic covered or on how the subject/topic may be connected to other situations. b. Entry slips- Short responses to questions posed at the beginning of class; c. Answering questions. One or few questions would be given which students must answer and submit for grading. d. Short summary. Students are asked to summarize main points of a reading assignment in a short paragraph. e. Group presentation- The tutorial class would be divided into groups and each group would be required to make a short presentation on a document provided. 14 6. In-course Test-20% One in-course test would be given worth 20%. The test would be given in week 12 of the semester and would comprise MCQs and structured questions. 7. Laboratory reports-20% Students would be required to submit 8 laboratory reports/completed worksheets for practical exercises. Each report/completed worksheet would be worth 2 marks. 8. Class participation- 5% Student participation in tutorial sessions will be monitored by the tutor and a grade assigned at the end of the semester. Participation would include more than just showing up at tutorial – some evidence of an active role in the tutorial discussion would be required to obtain the full 5% for participation. At the beginning of the semester, the students in each tutorial would engage in developing the exact assessment criteria for this assignment, and the studentdeveloped criteria will be used to determine each student’s participation grade. Cheating, including plagiarism, would not be tolerated. Incidents of cheating would be dealt with according to UWI’s rules and regulations including: - Examination Regulations for First Degrees, Associate Degrees, Diplomas and Certificates. Section (B) Cheating. Regulations 96–102; http://sta.uwi.edu/resources/documents/Exam_and_GPA_regulations.pdf. - University Regulations on Plagiarism (First Degrees, Diplomas and Certificates). http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf. EVALUATION: BIOL1263 will be evaluated in two ways – (a) through the offices of the Class Representative and the Life Sciences Student-Staff Liaison Committee, and (b) an end of semester course evaluation survey. The class will elect four class representatives (one per lab stream), whose role is to act as a mediator between the Life Sciences academic staff and the students in the class. The representatives will attend Liaison Committee meetings (held at least twice per semester), where they will present feedback on the course to the Department for action. The UWI performs a course evaluation survey at the end of every semester, and this information will also be used for overall assessment of the course and guide possible actions for improvement in subsequent semesters. 15 TEACHING STRATEGIES: A combination of teaching strategies will be adopted in BIOL1263. The primary teaching strategy will be based on the face to face classroom lectures and discussions. Videos of lecture presentations would be made available to students prior to the lecture session via podcast media. Students are advised to view these videos before the lecture so that more emphasis can be placed on discussions and answering questions, thereby facilitating deep learning. This would also afford the time to have assessment exercises on “writing across the curriculum”. MyeLearning will be utilized throughout the course as a means to provide access to course materials such as Powerpoint presentation files, animations, weblinks, lessons and quizzes. This medium would also be used as a portal for student-lecturer communications and the dissemination of coursework feedback. A total of six tutorial sessions would be give for the semester. These would be small group sessions which would be conducted by instructors or tutors, who would normally by senior postgraduate students in Life Sciences. Students must attend tutorial sessions. Tutorials sheets will be assigned prior to each session via myeLearning, and students are expected to attempt the solutions before coming to tutorial. The goal of the tutorial session is to give students a more hands-on experience with the course material and easier access to course instructors. Students will be expected to ask and answer questions on material that is unclear, propose solutions to questions on the tutorial sheet and to generally participate fully in the tutorial activities. Tutors will not merely be going through the answers to the tutorial questions in these sessions. Practical sessions would include demonstrations and problem solving exercises to develop practical skills and enhance understanding of content covered in the theory section. These exercises would include dissection and analysis of specimens, experiments and use of identification keys to assign organisms into different levels of taxa. In order to pass the course, you must gain a passing mark of 40% from both the coursework and the final theory exam. Any student who misses more than 25% of practical classes or tutorial sessions without a medical or other valid excuse can be debarred from writing the final exam. Recommended Text Hickman CP, Roberts LS, Keen SL, Larson A, and Eisenhower DJ (2007). Animal Diversity. Fourth edition. McGraw Hill Higher Education Useful reading Introductory Mycology by Alexopolus, Mims and Blackwell, 4th Edition, 1996, Wiley Useful websites 16 www.mhhe.com/hickmanad4e (animals): http://tolweb.org/fungi (fungi) Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 Course Calendar LECTURE TOPICS (by week) Week Practical Lecture/Tutorial 1 Lecture 1: Complexity, diversity and classification of animals; Origin of eukaryotes; Protozoa; Origin of the Metazoa Lecture 2: Porifera; Cnidaria 2 Tutorial 1 17 Lecture 3: Platyhelminthes; Bilateral symmetry; Triploblastic condition; Writing across the curriculum exercise 3 4 5 Dinoflagellates e.g. Trypanosomaslides Sarcodines e.g Amoeba Ciliates e.g Paramecium Apicomplexans e.g Plasmodium Lecture 4: Nematoda; Early embryonic development Body cavities; Lecture 5: Annelida: Body organization and phylogenetic; Placement; Life habits and diversity; Metamerism and tagmosis (tagmatization); Tutorial 2 Lecture 6: Writing across the curriculum exercise Porifera- Diversity of Poriferanssponges CnidariaLecture 7: Examination of live material- Hydra Obelia, Jellyfishes, sea anemones and Corals Arthropoda: Exoskeleton, adaptations to life and land Arthropoda: Metamorphosis and diversity Lecture 8: Mollusca: Body plan, diversity; Echinodermata Chordata: Origin and phylogeny; Invertebrate chordates PlatyhelminthesDiversity of Flatworms 18 TurbellariansBipalium MonogeneansGyrodactylus TrematodesFasciola Cestodes-Taenia Demonstration of preserved specimens 6 NematodesDiversity of nematodes Examination of Preserved Specimens e.g. slides Tutorial 3 Lecture 9: Chordata: Fishes Diversity Writing across the curriculum exercise Annelids – earthworms 7 InsectsCockroaches, Beetles, stink bugs, wasps, honey bees and flies Lecture 10: Chordata: Amphibians Diversity Lecture 11: Chordata: Reptiles diversity and adaptation to land PomaceaPernaShrimp Tarantula- 19 Star fish, sea urchin, Sea cucumber. 8 Molluscan Diversity Polyplacophora (Chitons) Gastropodssnails and slugs Tutorial 4 Lecture 12: Chordata: Birds; evolution and adaptation of flight; Chordata: Writing across the curriculum exercise Pelecypodabivalves- clams, oysters and mussels Cephalopodsoctopus and squids Arthropod Diversity Crustaceafiddle crab, woodlice, barnacles Arachnida-scorpions, spiders, ticks and mites Myriapodacentipedes, millipedes 9 Bufo marinus: Tadpoles- Hyla Freshwater fish Anolis Birds 10 Mammals Zygomycetes 11 Ascomycetes Basidiomycetes Oomycetes 12 Lecture 13: Mammals Lecture 14: Classification and diversity of fungi; importance of fungi to humans and the environment. Tutorial 5 Lecture 15: Chytridiomycota; Zygomycetes Lecture 16: Writing across the curriculum exercise Ascomycetes (and Deuteromycetes) Lecture 17: Basidiomycetes Lecture 18: Fungal-like organisms In course Test Tutorial 6 20 THE UNIVERSITY OF THE WEST INDIES ST. AUGUSTINE FACULTY OF SCIENCE AND AGRICULTURE DEPARTMENT OF LIFE SCIENCES Course Description Title: BIOL1362- Biochemistry I (This course replaces the Biochemistry component of BIOL1061 Cell Biology & Genetics) Credits: 3 (23 hours of Lectures, 8 hours of Tutorials and 12 hours of Practicals) Level: Undergraduate -Year I Semester: II Offering Department: Department of Life Sciences, Faculty of Science and Agriculture, The University of the West Indies, St. Augustine. Course coverage: Functions of organelles, endosymbiotic theory, limits on cell size; structurefunction relationships of the major classes of biomolecules; introductory enzymology; glycolysis and the metabolism of macronutrients. Pre-requisites: CAPE Biology (or equivalent). A solid background in chemistry is assumed and a pass in CHEM 1061 or CHEM 1062 is strongly recommended. Significance/Rationale: Biochemistry is a fundamental sub-discipline of Biology but also incorporates organic, inorganic and physical chemistry. Molecular biology has revolutionized every aspect of the study of Biology and a grasp of current and future trends in Biology, including Ecology, requires an appreciation of life at the molecular level. This begins with the study of basic concepts in Biochemistry. Enrolment capacity: 400 students (maximum) 21 Purpose of the Course This course provides an introductory treatment of concepts in Biochemistry. In many regards, students will be learning a vast new language as well as new insight into the molecular logic of life – how the structure/form of molecules is related to their diverse functions. At the end of the course, students are expected to have a basic understanding of the biochemical nature of life. Letter to the Student This course aims to render you literate in the discipline of Biochemistry – to lay the foundation for further study in biochemistry, genetics, microbiology, molecular biology, natural products and several other disciplines. You will build the vocabulary and gain a basic understanding of the biochemical concepts necessary to follow fast-paced developments in biology. The most cursory glance at any top-ranked journal in any of the Biological sciences will reveal the extent to which Biochemistry and Molecular Biology continue to revolutionize our thinking. Moreover, Biochemistry and Molecular Biology serve as unifying disciplines, impacting on fields as diverse as medicine, forensics, pharmaceutics, cosmetics, ecology, agriculture, biotechnology and anthropology to name a few. A career in science, whether as a researcher, science administrator, educator or in science popularization, requires that you be literate in this discipline. It is a demanding discipline that requires you to keep up with pace at which you will be introduced to the fundamentals and to practise its vocabulary and application to problems and questions of life as introduced in your tutorial exercises. Course Content and Objectives (Note: h* denotes lecture hour) TOPIC 1 - Cell Organelles (3h) Cell Theory and the Theory of endosymbiosis. Structure and function of cellular organelles: Endoplasmic reticulum, Golgi apparatus, Chloroplast, Lysosomes, Peroxisomes, Glyoxysomes, Mitochondria, Nucleus, cytoskeleton The proteasome. At the end of this section students should be able to: Describe the theory of endosymbiosis Explain how the structure of organelles in eukaryotic cells is related to their function 22 TOPIC 2 - Chemistry of Carbohydrates (3h) General formula. Monosaccharides – aldoses and ketoses, trioses, tetroses, pentoses, hexoses and heptoses. Representation of monosaccharide structures – Fischer and Haworth projection formulae, ring formation, furanose and pyranose forms. Stereoisomerism – chirality and optical activity, enantiomers, epimers, anomers, D and L designations. Glycosidic bond formation, common disaccharides and polysaccharides – sucrose, maltose, lactose, starch, glycogen, cellulose, chitin, hyaluronate and chondroitin sulphates. Glycoproteins – fish antifreeze protein, ribonuclease B and human IgG. At the end of this section, students should be able to: Identify ketoses and aldoses and explain the tests used to distinguish between them. Draw Fischer and Haworth projection formulae for named tetrose, pentose and hexose sugars. Define: stereoisomers, diastereoisomers, enantiomers, epimers, anomers and give named examples. Distinguish between D & L sugars. Identify chiral carbons and calculate the number of stereoisomers a molecule can have. Distinguish between hemiacetals, hemiketals, acetals and ketals and the significance to reducing and non-reducing sugars. Describe the oxidation and dehydration reactions of monosaccharides. Write reactions for glycoside formation, including ring formation. Identify and draw glycosidic bonds and identify the structures of common disaccharides. Relate polysaccharide structure to function Design a flow chart to elucidate the nature of unknown carbohydrates in the laboratory. TOPIC 3 - Chemistry of Lipids (2h) Definition of lipid. Fatty acids – nomenclature and physical properties, oxidation, reduction and ester formation. Triacylglycerols and waxes – structure and function. Structure and function of complex lipids: phosphoglycerides, sphingolipids, and glycolipids – membrane structure. Arachidonic acid and eicosanoid formation, cholesterol and derivatives. At the end of this section, students should be able to: Define the terms: lipid, amphiphilic (amphipathic) and differentiate between hydrolysable and non-hydrolysable lipids and between simple and complex lipids. State the roles of different kinds of lipids. Use different nomenclature to represent fatty acids. Relate structures of lipids to their physical properties and reactions. Relate structures of lipids to their functions. Identify the different classes of lipids from their chemical formulae 23 TOPIC 4 - Amino acid & Protein Chemistry (3h) Classification of the 20 common amino acids based on charge, polarity, aromaticity and affinity for water. Ionization of amino acids, pK values, the Henderson-Hasselbalch Equation, determination of pI values, Introduction to acid-base behavior. The peptide bond and protein structure. 1o, 2 o, 3 o and 4 o structure. Simple and conjugated proteins. Classification of proteins based on structure and function. At the end of this section, students should be able to: State the different classes into which amino acids may be grouped and give named examples of amino acids belonging to each class. Describe the acid-base behaviour and ionization of amino acids and define the term ampholyte. Calculate the pI of any amino acid given its pK values. Draw and interpret titration curves and utilize the Henderson-Hasselbalch Equation to predict the ratios of ionized species at given pH values. Describe the formation and characteristics of peptide bonds. Describe how pH and ionic strength affect protein solubility. Define what is meant by protein primary structure and outline the possible outcomes of altering amino acids within a protein. Explain what is meant by sequence homology and how it might be used to elucidate functions of proteins Describe protein secondary structures and the impacts of proline and glycine on secondary structure. Define tertiary and quaternary structures and briefly describe the importance of disulphide bridges. Describe the phenomenon of protein denaturation and renaturation. TOPIC 5 - Nucleotides and Nucleic Acids (2h) Composition – nitrogenous base, phosphate group and pentose sugar. Nucleosides and nucleotides –cellular functions. Pyrimidine and purine bases. Polarity of nucleic acids – 5 3 . DNA double helical structure: A-, B-, and Z-DNA, major and minor grooves, interaction with tra s riptio fa tors, ase pairi g a d ase sta ki g. Chargaff s o ser atio s. “ize a d organization of DNA. Some RNA structures. Central role of ATP in bioenergetics. Substrate-level phosphorylation. At the end of this section, students should be able to: Distinguish between nucleosides & nucleotides, between purines & pyrimidines and between RNA and DNA. 24 Outline the functions of nucleotides as: building blocks of nucleic acids, sources of energy used to drive unfavourable reactions and components of coenzymes Describe the nature of the phosphodiester bridge and how it gives polarity to polynucleotides and the labile nature of this bond in RNA relative to DNA Describe the factors which facilitate specific base pairing and state the observations on DNA composition made by Erwin Chargaff. Describe the features of the Watson-Crick model of DNA (B-DNA) including the factors which contribute to the stability of the double helix. Describe how B-DNA differs from A- and Z-DNA. Describe the factors which contribute to the flexibility of the DNA molecule Describe the spontaneous reactions which can take place in DNA: deamination, depurination and reactions between adjacent thymine bases Describe the hyperchromic effect and the effect of high salt concentration on the melting temperature of double-stranded DNA. Describe the factors which promote the hydrolysis of ATP TOPIC 6 - Enzymes (4h) The nature of enzymes. Specificity of enzyme action – the active site. Activation energy and transition states. Initial velocity and substrate concentration. The Michaelis-Menten Equation. Determination of Vmax and KM values - the Lineweaver-Burk plot, the Eadie Hofstee plot. Enzyme inhibition- reversible inhibition: competitive, uncompetitive and non-competitive inhibition, allosteric inhibition, substrate inhibition. Quantitation of enzyme activity – international units, S.I. units and specific activity. At the end of this section, students should be able to: Define the terms: apoenzyme, holoenzyme, coenzyme, prosthetic groups, isoenzyme, metalloenzyme, metal-activated enzyme, active site, transition state, activation energy. Briefly describe different hypotheses proposed to explain enzyme specificity and catalysis. List the mechanisms which are used to regulate enzyme activity Briefly describe the six broad groups into which enzymes are classified and give named examples from each class Distinguish between absolute and group specificity and describe the properties of enzyme active sites Use the Michaelis-Menten and Lineweaver-Burk equations to determine kinetic parameters for enzymes and relate them to the equation of a hyperbolic curve and a straight line respectively List the assumptions inherent in the derivation of the Michaelis-Menten equation Distinguish between different kinds of reversible enzyme inhibitors based on the mode of binding as well as on the effect of the inhibitor on enzyme kM and Vmax values. Describe how and why pH and temperature affect enzyme activity Use enzyme units to define enzyme activity. 25 TOPIC 7 - Glycolysis (3h) Anaerobic breakdown of hexose sugars to trioses. The pathway and its regulation through allosteric and hormonal control of phosphofructokinase, hexokinase and pyruvate kinase. Net energy yield from glycolysis. Entry of fructose and galactose into glycolysis. End product of glycolysis – pyruvate, lactate or ethanol? At the end of this section, students should be able to: List the types of cells/tissues which must rely on glycolysis to provide energy and explain the reasons for this dependence State the other purposes served by this pathway List the names of enzymes catalyzing each reaction List the substrates and products of each reaction Describe the sub-cellular location of the pathway and how the cofactors are regenerated State the sites where ATP is generated Describe how the pathway is regulated Describe what happens in the pathway under aerobic and anaerobic conditions: what products are formed and what cofactors are limiting TOPIC 8 - Overview of Metabolism (2h) Catabolism of amino acids, lipids and carbohydrates leads to generation of Acetyl CoA. Common fate of acetyl CoA – The tricarboxylic acid cycle and the electron transport chain. Synthetic paths from the TCA. At the end of this section, students should be able to: Show how acetyl-CoA is central to the catabolism of the macronutrients protein, carbohydrates and lipids. Describe how the tricarboxylic acid cycle generates reducing power Describe how the electron transport chain utilizes reducing power to generate an electrochemical gradient that is in turn used to generate chemical power in the form of ATP Assignments Tutorial sheets on each topic to be prepared in written format Preparation of Laboratory Reports 26 In-Course Assessment Laboratory reports 20% In-course tests 20% Total 40% Evaluation Course work 40% 2-hr. final Theory- 60% MCQ s, stru tured a d essa Teaching Strategies These include lectures and other supporting materials on myelearning, as well as small group tutorial sessions. In course tests are cumulative to encourage students to keep abreast of the material as presented and also to retain work done early in the semester. Case-studies will be employed where appropriate to encourage problem-solving and critical thinking skills. Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 27 Essential Text -Any one of: 1. Lehninger Principles of Biochemistry by David L. Nelson, Michale M. Cox and Albert Lehninger. 6th Edition. 2. Biochemistry by Jeremy M. Berg, John L. Tymoczko and Lubert Stryer, 7th Edition. 3. Principles of Biochemistry by H. Robert Horton 4th Edition (ISBN 0131453068). BIOL 1362 COURSE CALENDER WEEK 1 LECTURES 1 Cell Organelles 2 Cell Organelles PRACTICALS Tutorial 2 3 Cell Organelles 4 Carbohydrate chemistry 1. Virtual Lab – Cell Organelles TUTORIAL 3 5 Carbohydrate chemistry 6 Carbohydrate chemistry Review of Exercise 1 TUTORIAL 4 7 Lipid chemistry 8 Lipid chemistry 2. Carbohydrates & Lipids TUTORIAL 5 9 Amino Acids & Proteins Review of Exercise 2 In-Course Examination # 1 28 TUTORIAL 6 10 Amino Acids & Proteins 11 Amino Acids & Proteins 3. Preparation and assay of phenolase and peroxidise from sweet and Irish potato TUTORIAL 7 12 Nucleic acids 13 Nucleic acids Review of Exercise 3 TUTORIAL 8 14 Enzymes 4. Demonstration of Dehydrogenase enzyme activity 15 Enzymes TUTORIAL 9 16 Enzymes 17 Enzymes TUTORIAL 10 18 Glycolysis Review of Exercise 4 19 In-Course Examination #2 TUTORIAL 11 20 Glycolysis 21 Glycolysis TUTORIAL 12 22 Overview of metabolism 23 Overview of metabolism TUTORIAL 29 THE UNIVERSITY OF THE WEST INDIES ST. AUGUSTINE FACULTY OF SCIENCE AND AGRICULTURE DEPARTMENTOF LIFE SCIENCES Course Outline Course Title: Introductory Genetics Course Code: BIOL 1364 Credits: 3 Level: ONE Semester: TWO Pre-requisites & Assumed Knowledge: CAPE Biology OR BIOL 0061: Preliminary Biology I and BIOL 0062 Preliminary Biology II Students are expected to have a prior basic knowledge and understanding of the following: Chromosomes Mitosis & Meiosis Prokaryotes & Eukaryotes DNA structure & replication Course Descritpion This course aims to present an introduction to the basic principles of Genetics and will equip students with the necessary foundation for advanced level courses in Biology & Biochemistry at the Department of Life Sciences, University of the West Indies. The course content has only been slightly modified from what has been previously taught in the Genetics component of “BIOL 1061 – Cell Biology & Genetics”. The previously taught BIOL 1061 delivered all course content in just six weeks which is too intensive of an approach. This course is being implemented to maintain the current requirements for the Biology major while offering students more flexibility and time to assimilate concepts being presented. Major topics to be covered include: The Nuclear Genome, The Cell Cycle & Mendelian Genetics, Extensions of Mendelian Genetics, including the molecular basis of heredity, Linkage analysis & Gene Mapping, Sex Linkage & Sex Determination, The Extrachromosomal Genome and Extrachromosomal Inheritance. Students will have the opportunity to work on problem sheets to calculate genotypic and phenotypic ratios and to access simulations of mitosis and meiosis as well as drosophila transmission Genetics on-line Feed- back sessions will take place after each In- course Test. 30 The course consists of 23 (50-min) lectures 3 hrs of In-Course Tests, supplemented with 6 tutorial sessions. In addition students will have the opportunity to gain experience in the laboratory through implementation of 5 three-hour practical sessions. Lectures and Tutorials, Review and feedback sessions: Practicals: 31.5 credit hours 7.5 credit hours (3 x 5 = 15 hours/2) Course Assessment ASSESSMENT In-course Examination #1 In-course Examination #2 Laboratory/Practical Exercises (3) Tutorial Question Sheets Final Examination Total WEIGHTING 10% 15% 15% 10% 50% 100% Course Calendar Practical sessions held in alternate weeks Week Lecture subjects Tutorials and Labs. 1 (3h) Lect1: Course Overview & Introduction to Genetics 2 (5h) Lect.#3: Introduction to the Human Genome and the Cell Cycle Lab. #1- Basic Microscopy & Visualization of Lect.#4: Mitosis & Meiosis Chromosomes (3h) 3 (3h) Lect.#5: Mendelian Patterns of Inheritance 4 (5h) Lect. #7&8: Intra-allelic Interactions Tutorial #1 Lect. #2: Chromosome Structure & Morphology Tutorial #2 Lect.#6: Predicting Inheritance & Goodness-of-Fit Testing In-course Test #1 Lab.#2Chromosome staining (3h) 31 Week Lecture subjects Tutorials and Labs. Tutorial #3 Feedback on InCourse Test#1 5 (3h) Lect. # 9&10: Inter-allelic Interactions 6 (5h) Lect. #11: Environmental Effects on Mendelian Inheritance 7 (3h) Lect. # 12: Multiple Allelism Lab.#3Drosophila Transmission Genetics (3h) Lect.#13 :Polygenic Traits & Quantitative Inheritance Tutorial #4 Lect. #14 :Introduction to Linkage & Linkage Relationships 8 (5h) Lect. #15: Linkage Mapping Lect. # 16: Linkage Analysis in Ascomycetes 1 In-Course Test #2 Lab.#4- ChiSquare Problem Solving 9 (3h) Lect. # 17 :Linkage Analysis in Ascomycetes 2 Tutorial #5 Lect.# 18: Sex Determination Systems in Animals Feedback on InCourse Test#2 10 (5h) Lect. # 19: Sex Determination Systems in Plants In-Course Test #3 Lect. # 20 : Sex Linkage & Inheritance of Sex-linked Traits Lab#5- Linkage Analysis Problem Sheets 11 (3h) Lect. # 21: Pedigree Analysis Tutorial#6 Lect. # 22: Extrachromosomal Inheritance 1 Feedback on InCourse Test#3 12 (3h) Lect. # 23 :Extrachromosomal Inheritance 2 None 13 None Lect. # 24 Revision Lecture (1h) 32 Lecture Outline & Learning Outcomes Lecture 1: An Introduction to Genetics A definition of genetics Branches of genetics DNA is the genetic material By the end of this lecture students should be able to: Describe what the study of genetics entails and give an outline of the various branches of study in the subject field. Explain why the genetic material can either be DNA or RNA Describe the crucial experiments that proved nucleic acids as the genetic material of living organisms Lectures 2, 3 & 4: Chromosomes & Heredity Chromosome structure & morphology Chromosome staining & banding nomenclature The human genome Features of homologous chromosomes Defining key terms The cell cycle Mitosis & Meiosis By the end of these lecture students should be able to: i. ii. iii. iv. v. vi. vii. viii. Distinguish between DNA and chromosomes and explain the chromosome theory of inheritance. Describe the basic morphology of chromosomes and define and illustrate metacentric, submetacentric, acrocentric & telocentric chromosomes. Describe G-banding as a chromosomal staining technique that produces specific banding patterns. Use the established nomenclature for the identification of specific chromosomal regions and sub-regions. Describe the human genome as comprising 22 pairs of autosomes and a pair of sex chromosomes. Explain the term ploidy using humans as examples of diploids Describe the features of homologous chromosomes. Define the following key terms: gene, allele, locus, Central Dogma, dominant, recessive, homozygous, heterozygous, genotype, phenotype, variation & mutation. 33 ix. x. xi. xii. Outline the different phases of the eukaryotic cell cycle (G0, G1, S, G2 & M phases) and describe what is taking place in each of these phases. Describe in detail and illustrate the processes of mitotic and meiotic cell division and compare these two processes, commenting on the significance of each. Distinguish between random chromosome assortment & crossing over as the basis of recombination. Explain how variation is generated and clearly distinguish between the processes of recombination & mutation. Lectures 5 & 6: Mendelian Genetics Mendel’s Laws & The Law of Product Probability Monohybrid Inheritance Dihybrid inheritance Trihybrid Inheritance Test-crosses Predicting Inheritance Goodness-of-fit Testing (Chi-Square Test) By the end of these lectures students should be able to: i. ii. iii. iv. v. vi. vii. Compare the behavior of chromosomes in meiosis with that of ‘Mendel’s particles’. State Mendel’s laws: (The law of segregation and the law of independent assortment). Predict the outcome of monohybrid, dihybrid and trihybrid crosses using Mendel’s laws, the law of product probability and by application of the Punnet Square. Show how the inheritance of complex characteristics (monogenic, digenic, oligogenic & polygenic traits) can be resolved by using the law of product probability and the branch method. Explain the purpose of the test-cross and apply it to determine unknown parental genotypes. Test predicted outcomes of crosses and expected Mendelian phenotypic ratios against empirical values by employing the chi-square test. Predict the inheritance of characteristics by building hypothetical models and testing them. Lecture 7&8: Extensions of Mendelian Inheritance – Intra-allelic Interactions Modifications of expected Mendelian phenotypic ratios by interaction between alleles of the same gene controlling a single trait: a. Complete dominance b. Incomplete dominance c. Co-dominance d. Over dominance e. No dominance 34 f. Lethal allelic relationships By the end of these lectures students should be able to: i. ii. iii. Explain the non-conformance of empirical phenotypic ratios with expected Mendelian ratios and list the modifying factors that may cause them to vary significantly. Demonstrate that intra-allelic interactions stem from the relationship between alleles of the same gene. Outline and explain the various intra-allelic relationships and the underlying biochemical basis for each of these interactions, clearly showing in each case how they modify expected Mendelian phenotypic ratios. Lecture 9 & 10: Extensions of Mendelian Inheritance – Inter-allelic Interactions Modifications of expected Mendelian phenotypic ratios by interaction between alleles of different genes controlling a single trait: a. Recessive epistasis b. Duplicate recessive epistasis c. Dominant epistasis d. Duplicate dominant epistasis e. Dominant/Recessive interaction f. Duplicate interaction By the end of this lecture students should be able to: i. ii. iii. Recognize that inter-allelic interactions stem from the relationship between alleles of different genes. Describe the different classes of epistasis (complementary, inhibitory and duplicate) and the types of epistases within each of the classes. Outline and explain the various inter-allelic relationships and the underlying biochemical/ molecular basis for each of these interactions, clearly showing in each case how they modify expected Mendelian phenotypic ratios. Lecture 11& 12: Extensions of Mendelian Inheritance Environmental Effects Multiple Allelism By the end of this lecture students should be able to: i. Explain how both external and internal environmental effects can alter gene expression patterns and by extension modify phenotypes/ phenotypic ratios. 35 ii. iii. iv. v. vi. vii. Provide examples of modifying environmental effects and explain how they operate. Define and distinguish between ‘gene penetrance’ and ‘expressivity’ and explain their use as measures of the extent to which a gene manifests itself under varying environmental conditions giving specific examples for each. Explain the concept of multiple alleles at a single gene locus and its effect on phenotypic variation within a population. Describe specific examples of multiple allelism, highlighting the dominance relationships between members of the multiple allelic series. Explain that although genes may have multiple alleles, the alleles obey the same rule of transmission. Describe how multiple allelism can lead to greater genetic variability in populations. Lecture 13: Polygenic Traits & Quantitative Inheritance Polygenic Traits and Polygenic Inheritance Concept Heritability and Heritability Estimates By the end of this lecture students should be able to: i. ii. iii. iv. v. Explain what is meant by the term polygenic traits and cite relevant examples of such traits. Distinguish between quantitative (oligogenic) and qualitative (polygenic) traits and between oligogenic inheritance and polygenic inheritance Explain the reasons why polygenic traits show a normal distribution Explain the differences between studying the inheritance of oligogenic vs polygenic traits. Define and distinguish between the terms ‘heritability in the broad sense’ and ‘heritability in the narrow sense’ and explain the significance of these measures to breeders. Lectures 14 & 15: Linkage analysis & Mapping Definition of Linkage & Linkage Relationships Two-point linkage mapping Three-point linkage mapping By the end of these lectures students should be able to: i. ii. iii. Distinguish between synteny and linkage Distinguish clearly between a genetic map vs physical map; and the advantages and disadvantages of each. Explain the consequences of linked genes. Distinguish between types of linkage, complete, incomplete and no linkage and explain the effect each has on the phenotypic ratios obtained in the F2 and testcross generations 36 iv. v. vi. vii. viii. ix. x. xi. xii. xiii. xiv. Explain the relationship between chiasma frequency and recombination frequency and type of linkage. Calculate recombination frequencies based on F2 or test-cross data Calculate the test cross ratio when the F2 ratio is given or vice versa Use the proper notation for representing linked genes and determine whether the linked genes are in coupling phase or repulsion phase. Predict outcomes when considering linked genes in both cis (coupling) configuration and trans (repulsion) configuration. Express recombination frequencies as percentages and use them to determine map distances between genes in map units (m.u.) or centi-Morgans (cM). Calculate and determine map distances between genes based on recombination frequencies obtained from either F2 data or test-cross data. Clearly indicate the advantages of three-point linkage analysis as compared to two-point linkage analysis. To conduct linkage analysis, using test cross data obtained by test crossing a triple heterozygote Apply the two-point (2 gene markers) and three-point system (3 gene markers) of mapping genes. Describe the use of genetic maps in breeding. Lecture 16 & 17: Linkage Analysis in Ascomycetes Overview of Fungal Reproductive Cycle in Ascomycetes Ordered Tetrad Analysis Unordered Tetrad Analysis By the end of this lecture students should be able to: i. ii. iii. iv. v. vi. vii. Describe the sexual reproductive cycle in Ascomycetes. Perform linkage analysis on the meiotic products contained within asci (sacs). Distinguish clearly between ordered tetrad analysis vs unordered tetrad analysis. Illustrate the difference between first and second division segregation types. Carry out linkage analysis and calculate map distances between genes based on ordered tetrad analysis. Illustrate the formation of parental ditypes, non-parental ditypes and tetratypes in fungi with unordered tetrads. Carry out linkage analysis and calculate map distances between genes based on unordered tetrad analysis. Lectures 18, 19 & 20 Sex Determination and Sex Linkage Sex Determination Systems 37 Sex chromosome based inheritance By the end of these lectures students should be able to: i. ii. iii. iv. v. vi. vii. viii. ix. x. Define the term sex chromosome vs autosomal chromosomes Describe the sex determining mechanisms in the animal kingdom – sex chromosome mediated methods viz: XY-XX; XO-XX; ZW-ZZ; Z0-ZZ; Chromosome Balance Theory of Sex Determination; haplo-diploidy; haplo-diploidy and complementary genes; genic control of sex. Explain the following mechanisms of sex determination using appropriate examples to illustrate: a. Mammalian XY-XX system b. Protenor XX-XO system c. Birds ZW-ZZ system d. ZO-ZZ system e. Sex Balance Theory (genic balance) f. Yeast (MATa – MATα mating types) g. Environmental sex determination Distinguish between the terms homogametic and heterogametic sex Describe Morgan’s experiments involving the transmission of sex-linked traits in Drosophila melanogaster. Describe the sex determining mechanisms in various plants. Distinguish clearly between Monoecy and Dioecy. Distinguish clearly between autosomal vs sex linked vs holandric gene inheritance Describe and illustrate the criss-cross mode of inheritance as it relates to sex-linked traits. Illustrate and compare the differences observed in the outcomes for reciprocal crosses involving sex-linked traits. Lecture 21: Analysis of Sex-Linked Traits in Humans X-linked Recessive Inheritance X-linked Dominant Inheritance Y-linked Inheritance Pedigree Analysis By the end of this lecture students should be able to: i. ii. iii. iv. Explain the mechanisms by which X-linked and Y-linked traits are inherited. Distinguish between the inheritance patterns observed for X-linked recessive, X-linked dominant, Y-linked, autosomal recessive and autosomal dominant traits. Define and state the purpose of pedigree charts. Analyze and interpret the structure of pedigree charts and the associated symbols with an aim of determining the mode of transmission of a particular trait. 38 Lectures 22 & 23: Extrachromosomal Inheritance Chloroplast & Mitochondrial Genomes Features of Cytoplasmic Inheritance Nuclear-Cytoplasmic Interaction Maternal Effects By the end of this lecture students should be able to: i. ii. iii. iv. v. vi. vii. viii. Relate the genomes of prokaryotes to organellar genomes Describe the structure and organization of organellar genomes (chloroplasts & mitochondria). Distinguish between nuclear genomes and extra-chromosomal genomes Distinguish between nuclear and extra-chromosomal inheritance using appropriate examples to illustrate. Explain nuclear-cytoplasmic gene interaction using a named example. Define the term maternal effect and clearly explain the underlying mechanism of inheritance involved using appropriate examples. Distinguish between maternal effect and extra-chromosomal inheritance. Distinguish between diminishing and non-diminishing maternal effects. Lecture 24: Review Session Practical Exercises 1. Introduction to Basic Microscopy: Visualization of different stages of mitosis and meiosis By the end of this laboratory exercise students should be able to: - Know the parts of the microscope and thereby employ proper usage - Identify the different stages of mitosis and meiosis from pre-prepared slides - Draw accurate diagrams of chromosomes and their arrangement at the different stages of mitosis and meiosis - Calculate magnification values for drawings 2. Chromosome Staining By the end of this laboratory exercise students should be able to: - Employ the Feulgen staining technique to prepare fresh slides for the visualization of chromosomes in different plant tissues - Identify sources of error and employ precautions to ensure good squash preparations are obtained 39 - Identify the different stages of mitosis and meiosis from the newly prepared slides - Calculate the mitotic index and estimate the time spent in various stages of the cell cycle 3. Drosophila Transmission Genetics (Virtual Laboratory Exercise) Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 Recommended Texts 1. Brooker, Robert J. Genetics: Analysis & Principles 3rd Edition McGraw Hill Higher Education 2008. ISBN: 9780071287647 2. Snustad, D. Peter & Simmons, Michael J. Principles of Genetics 5th Edition John Wiley & Sons Inc. 2009. ISBN: 9780470388259 40 THE UNIVERSITY OF THE WEST INDIES, ST. AUGUSTINE FACULTY OF SCIENCE & TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE DOCUMENTATION COURSE CODE: BIOL 2061 COURSE TITLE: Cell & Developmental Biology CREDITS: 3 LEVEL: II SEMESTER: I PRE-REQUISITE(S): BIOL1263 Living Organisms II or BIOL1261 Diversity of Organisms or (BIOL1065 Diversity of Plants and Animals and AGRI1012 Microbiology) and either BIOL1362 Biochemistry I and BIOL1364 Genetics I or BIOL1061 Cell Biology and Genetics COURSE DESCRIPTION The course begins with a review of the structure and function of cellular membranes and organelles and the role of the cytoskeleton in cell shape and motility. The fundamental processes operating during embryonic development and cellular differentiation of plants and animals will then be examined. The principles of development will be considered at the organismal, cellular and molecular levels for a complete understanding of developmental processes. Students will be introduced to important experiments that have led to an understanding of the basic principles of development. The application of stem cells in research and associated ethical considerations will form the basis of class discussions and debates. COURSE RATIONALE Cell and Developmental Biology is a core course for the Biology program in the Department of Life Sciences. It serves to build upon the student’s basic knowledge of cell biology, and to introduce students to the fundamental concepts of developmental processes in several model organisms. Overall, this course is an essential component of the core biology degree as it examines the underling processes in a variety of model systems applicable to all major fields of biology. 41 INSTRUCTOR INFORMATION Name of course coordinator: Dr. Georgette Briggs Office address and phone: Biochemistry Office, Floor 2, Old wing, Natural Sciences Building Email address: [email protected] Office hours: Fridays 1:00-2:00 pm Preferred method of contact: Email Communication policy: Students should use their UWI email account for communication and can expect a response within 48 hours. COURSE GOALS: On completion of this course, students should have: An understanding of the fundamental processes operating during key developmental processes An appreciation of the role of developmental processes in disease The ability to critically analyze scientific experiments and their findings Gained skills in presenting and defending a logical, scientifically supported argument. CONTENT Developmental biology overall, seeks to understand the complexity that underlies the different cell types and organs, the emergence of form and patterns, and the communication between cells to achieve these processes. Almost every disease is linked to a developmental failure. As such, a portion of this course focuses on models that examine the links between basic developmental errors and disease. LECTURE OUTLINE AND LEARNING OUTCOMES Students completing this course should be able to: Lecture 1: Structure & Function of Biological Membranes Explain the general properties of biological membranes. Describe the molecular components of biological membranes. 42 Explain the role of various phospholipids in membrane structure. Describe the properties of the phospholipid bilayer. Explain how proteins are associated with membranes and outline some of their key functions. Describe the basis of membrane fluidity and its importance. Describe the nature of membrane asymmetry and its functional significance. Describe the role of membrane carbohydrates. Describe the electrical property of membranes and explain its significance. Describe mechanisms of transport across membranes using specific examples of channels and carriers. Lecture 2: Mitochondria & Chloroplasts Describe and illustrate the basic structural features of mitochondria. Give an overview of mitochondrial function. Describe and illustrate the basic structural features of chloroplasts. Give an overview of chloroplast function. Discuss the evolutionary origin/development of mitochondria & chloroplasts. Lecture 3: The Nucleus Describe the basic structure of the nucleus and its components. Describe the structure of the membranes comprising the nuclear envelope and explain its relationship to the endoplasmic reticulum. Discuss the evolution of the nucleus. Describe the composition of the nuclear matrix. Describe the structure, composition and function of the nucleoli. Describe the structure and function of nuclear pore complexes. Explain the mechanism of transport across nuclear membranes. Lecture 4: The Endoplasmic Reticulum & Golgi Apparatus Describe the structure of the Smooth and Rough ER. Compare and contrast the major functions of the Smooth and Rough ER. Describe the structure of the Golgi apparatus. Explain the major functions of the Golgi apparatus. Describe the processing of membrane proteins from the ER to their destination via the Golgi apparatus. Lectures 5 & 6: Secretion & Exocytosis and Endocytosis Define exocytosis as the terminal stage of secretion leading to the release of newly synthesized proteins from the cell Describe the different types of proteins and cells typically involved in secretion Explain the movement of proteins through the secretory pathway with particular emphasis on the role of the ER and the Golgi membranes in protein sorting & glycosylation 43 Describe the internal signal sequences in newly synthesized proteins responsible for their translocation and determining their ultimate destination Outline the glycosylatic reactions essential for passage through the secretory pathway Explain the process of vesicle formation and describe the role of various coat proteins involved Describe the motor protein mediated movement of vesicles along microtubules Define endocytosis as the transport of extracellular material into the cell. Describe the different mechanisms of endocytosis (phagocytosis, pinocytosis & receptor-mediated endocytosis) and their functions and roles in different cell types. Explain the role of clathrin in the invagination process of receptor-mediated endocytosis. Lecture 8: Endosomes, Lysosomes & Peroxisomes Describe the processing of early endosomes including the sorting of their components and recycling to the cell surface. Explain the role of lysosomes in the processing of endosomes. Describe the structure, function and origin of the peroxisome. Describe the oxidative reactions of the peroxisome. Explain the role of signal sequences and the mechanism of import of proteins into the peroxisome. Highlight the importance of peroxisomal function and its involvement in Zellweger syndrome. Lectures 9 & 10: The Cytoskeleton & Organelle Movement Define the cytoskeleton. Describe the subunit composition and assembly characteristics of each of its constituent elements and their roles. Explain the role of the cytoskeleton in normal cellular function including its association with key regulatory binding proteins. Identify and explain the role of key cytoskeletal structures and proteins responsible for enabling and regulating organelle and chromosome movement in the cell. Explain the role of dynein and kinesin in the mechanism of organelle movement along microtubules. Explain the critical role of motor proteins and cytoskeletal protein turnover in sorting replicated chromatids to daughter cells in mitosis. Describe examples of human diseases associated with cytoskeletal protein dysfunction. Lecture 11: Cell Motility Identify actin rich structures within the cell that are important for cell motility. Describe the organization of actin into specialized structures associated with cell motility and cell shape. Explain the role of cytoskeletal proteins and actin microfilament turnover in cell motility Describe the structure of cilia and flagella. Explain how organized assemblies of actin filaments and motor proteins work in combination to drive cell movements. Describe the basic structure, function and dysfunction of the axoneme. 44 Lecture 12: The Extracellular Matrix Identify the major components of the Extracellular Matrix (ECM) and describe their molecular structure, roles and distribution in specific tissues Describe the structure of integrins and their interaction with specific components of the ECM. Describe specific examples of clinically important conditions involving ECM-integrin interactions emphasizing how integrin dysfunction may be related to disease conditions Relate actin-based cell motility to ECM/integrin complexes. Lecture 13: Cellular Junctions & Cell Adhesion Identify and illustrate the appearance of the major types of cell junctions. Describe the molecular structure of the major types of cell junctions. Explain the distribution of cell junctions in relation to their functions in multicellular organisms. Highlight the importance of cell junctions and describe specific diseases in humans involving cell junction abnormalities. Lectures 14, 15 & 16: Cell Communication Describe the signals (chemicals & signaling molecules) involved in controlling cell function Define the terms ligand and receptor Describe the main cell surface receptor superfamilies (ligand-gated ion channels, G-protein coupled receptors, enzyme-linked cell surface receptors) and intracellular recptors Explain in outline the mechanisms of signal transduction including the generation of 2nd messengers (cyclic AMP, cyclic GMP and the phosphoinositide pathway) Explain the action of various signaling molecules on intracellular recptors in relation to the changes induced Identify and explain the intracellular mechanisms of the five major families of signal proteins primarily involved in the induction of animal development (Receptor tyrosine kinases, TGFβ superfamily, Wnt, Hedgehog, Delta-Notch) Lectures 17 & 18: Polarity & Development Explain the role of regulatory DNA in defining specific programs of development. Explain the role of morphogens and their interplay with extracellular inhibitors in defining cellular development. Explain the role of maternal effect genes in the organization of asymmetric egg division. Explain the specific cell-cell interactions involved in patterning and the role of developmental signals in cell differentiation (Wnt, Delta-Notch signalling). Explain the role of heterochronic genes in controlling the timing of development. Explain the role of apoptosis in developmental programs 45 Lecture 19: Stem Cells & Ethics Define the term potency (unipotency, oligopotency, multipotency, pluripotency, totipotency, terminally differentiated) as it relates to stem cells, cellular development and specialization. Discuss some of the ethical concerns associated with stem cell research & experimentation. Lectures 20 & 21: Embryogenesis Describe the stages of fertilization with particular emphasis on the molecular changes occurring in the formation of the zygote. Describe the morphological and molecular changes occurring in the transition phases of development from zygote through morula to blastocyst, the inner cell mass and trophoblast. Explain the fates and roles of the cells of each of the three germ layers: ectoderm, endoderm and mesoderm in the formation of specific tissues (myotome, sclerotome, nervous tissue & respiratory system). Lectures 22 - 27: Models of Development Caenorhabdatis elegans: Model of Development Drosophila melanogaster: Model of Body Plan Patterning Arabidopsis thaliana: Model of Development Lectures 28 & 29: Organogenesis & Appendage Patterning Identify the regulatory genes involved in defining cells fated to appendage development Explain the role of Wingless, Hedgehog, Dpp & Notch signaling in the patterning the wing disc Highlight some of the mechanisms involved in patterning vertebrate (Gallus gallus model) limbs Lecture 30: Problems of Development Highlight relevant case studies relating to problems in development COURSE ASSESSMENT In-course test 1: 10 % In-course test 2: 10 % Written Paper: 10 % Ethics Debate (on-line) 10 % On-line Discussion Forums 10 % Final examination (2 hours) 50 % 46 EVALUATION The elected Class Representative and/or Deputy will attend meetings with the course teacher(s) organized at every 4th week, and present feedback from the students attending that course or stream. This feedback is normally provided both orally and in written form for transmission to the lecturer. Apart from that the representatives will be attending the Liaison Committee meeting, and give their feedbacks to the committee. Students may comment on any aspect of the course or facilities. Students will be encouraged to submit their feedback (oral/written) during tutorials directly to the course teacher and appropriate actions will be taken by the teacher then and there. Results of in-course tests, and feedback of online discussion forums will be presented to the class. This will help students to check their progress constantly and also helps the instructor to identify the weak areas and thereby could alert and advise students individually to alter their approach of study and completing the work. The final reflective feedback and comments about the entire course and teaching will be collected on the last day of the course. This will be saved for analysis and utilized as a base for improvement for the next offering in the following year. COURSE DELIVERY & TEACHING STRATEGIES The course will be delivered via 28 one-hour lectures and supplemented with 3 tutorial sessions In addition students will have the opportunity to gain skills in oral communication, argument formation and execution (debating) and collaborative group research and presentation. The course would be delivered via lectures and tutorials. Online forum discussions & debates, written reports, and virtual labs, would also be used to encourage student participation and collaborative learning. COURSE CALENDAR WEEK 1 Lecture/Tutorial Lecture 1: Course introduction and review of structure & function of Biological Membranes Lecture 2: Mitochondria & Chloroplasts Lecture 3: The Nucleus 2 Lecture 4: The Endoplasmic Reticulum & Golgi Apparatus Lecture 5 : Secretion & Exocytosis Lecture 6: Secretion & Exocytosis 47 Activity 3 Tutorial Lecture 8: Endosomes, Lysosomes & Peroxisomes Topics given for written assignment Lectures 9 : The Cytoskeleton & Organelle Movement 4 Lectures 10: The Cytoskeleton & Organelle Movement Lecture 11: Cell Motility Online discussion forum Lecture 12: The Extracellular Matrix 5 Tutorial Lecture 13: Cellular Junctions & Cell Adhesion In course exam In-course #1 (Weeks 1-4) 6 Lecture 14: Cell Communication Online discussion forum Lecture 15: Cell Communication Lecture 16: Cell Communication 7 Lecture 17: Polarity & Development Lecture 18: Polarity & Development Written paper due Lecture 19: Stem Cells & Ethics 8 Tutorial Lectures 20: Embryogenesis Lecture 21: Embryogenesis 9 In-course #2 (Weeks 5-8) In course exam Lecture 23: Models of Development Lecture 23: Models of Development 10 Lecture 24: Models of Development Online discussion forum Lecture 25: Models of Development Lecture 26: Models of Development 48 Lecture 27: Models of Development 11 Lecture 28: Organogenesis & Appendage Patterning Online ethics debate Online Ethics Debate Lecture 29: Organogenesis & Appendage Patterning 12 Lecture 30: Problems of Development Lecture Course Review Tutorial 13 Course Review Tutorial Recommended Texts Developmental Biology, Scott F. Gilbert, 9th Edition; Sinauer Associates, Inc. 2010 Molecular Biology of the Cell, Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, and Peter Walter. 4th Edition; 2002 Other Texts Patterns & Experiments in Developmental Biology, Johnson & Volpe 3rd Edition; McGraw-Hill companies, Inc. 2001. ISBN 0-07-237965-0 Embryology: An Introduction to Developmental Biology Stanley Shostak 1991. Harper/Collins, New York ISBN 0-06-046126-8 Principles of Development Lewis Wolpert, Rosa Beddington, Thomas Jessell, Peter Lawrence, Elliot Meyerowitz, Jim Smith. 2nd Edition, 2002. Oxford University Press ISBN 0-19-924939-3 Cells, Embryos And EvolutionJohn Gerhart and Marc Kirschner 1997Blackwell ScienceISBN 0-86542-574-4 ADDITIONAL INFORMATION Student Attendance Attendance in the in-course exams and participation in the online discussions and ethics debate are mandatory. Any student who misses any of the mandatory activities, is advised to consult immediately 49 in person or by email with the course instructor regarding their make-up options. Absence must be accompanied by a written excuse or medical submitted to the Main office, Life Sciences within 7 days of the missed session. Any student who was inexcusably absent or who does not write an incourse exam or a quiz will receive 0% for that exercise. How to study for this course Students are encouraged to work together in small cohesive groups as much as possible to go through the course content. As we go through the various topics, students should attempt to answer all the sample questions placed on myelearning and discuss the answers amongst themselves. All comments, questions and concerns provided on a particular topic will be addressed during the discussion segments of laboratories and during class time. To support the material presented in class, several texts have been recommended. These texts would also be supplemented by various scientific journal articles available to you via the myelearning platform. Use the responses and comments for your quizzes and in-course examinations as a guide to answering the questions properly. There are several past paper questions in the library and students are encouraged to practice these questions. Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 50 THE UNIVERSITY OF THE WEST INDIES, ST. AUGUSTINE FACULTY OF SCIENCE & TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE DOCUMENTATION COURSE OUTLINE COURSE CODE & TITLE: BIOL 2262 - EVOLUTIONARY BIOLOGY NO. OF CREDITS: 3 LEVEL II SEMESTER I PREREQUISITES: BIOL 1364 AND any two of the following: BIOL1262, BIOL1263, or BIOL1362 COURSE DESCRIPTION Concepts of evolutionary biology are the basis of understanding all of modern biology. Following a short historical introduction, about one-quarter of the course is devoted to population genetics and evolutionary mechanisms at the population level. This leads to treatment of the nature of species, the history of life and past evolutionary patterns, special forms of evolution and phylogenetic analysis. The basic teaching/learning approaches include lectures, tutorials and practical exercises in the lab and field. Assessment is designed to encourage students to work continuously with the course materials, explore and critically analyse research in this dynamic field. COURSE RATIONALE This course is designed to provide the foundation of modern evolutionary biology that must be at the heart of all biological education. It is a core course for all students in the B.Sc. General majoring in Biology or in the B.Sc. (Special Option) Biology with Specialisations. INSTRUCTOR INFORMATION Name of course coordinator: To be assigned Office address and phone: Email address: Office hours: Preferred method of contact: Communication policy: Students should use their UWI email account for communication and can expect a response within 48 hours. LETTER TO THE STUDENT Dear student, Welcome to BIOL 2262 Evolutionary Biology. This is a core course in the Biology degree which serves to provide a basic foundation for students to understand the mechanisms of change in living 51 organisms or biological evolution. It attempts to answer some of the simplest questions e.g. How did life originate? Why are there so many species on Earth? You will be exposed to modern evolutionary biology which is based on new methodologies in genetics and molecular biology, rigorous application of the scientific method, and observational and experimental evidence from a variety of field and lab situations. You will be expected to refer to a broad range of topics in biology from the molecular to the geological scales e.g. molecular biology, population genetics, anatomy, physiology, behaviour and ecology. In addition, we expect you to think like a practicing scientist in one of the broadest and most complex fields in biology i.e. to apply the principles of the scientific method, to evaluate your own ideas and critique those of others, think analytically and solve problems. On completion of this course you will be able to apply evolutionary concepts to all aspects of biology and see how evolution shapes even the future of our own species. Please read the Course Outline carefully and keep the Course Schedule close to hand as it contains all the activities and assessments during the semester. In particular take note of the policies on plagiarism and attendance requirements. We are here to ensure your success in this course and at UWI generally and encourage you to come to us with any academic or other challenges you may face that could affect your attendance and performance. We look forward to meeting each of you and engaging in productive stimulating discussions over the coming semester. Course Coordinator CONTENT: BIOL2262 includes the following topics: the development of ideas in evolutionary biology; processes leading to change within populations i.e. microevolution (including modifications of the Hardy-Weinberg equilibrium, natural selection, random genetic drift, molecular evolution); large scale processes at the geological time scale i.e. macroevolution; key events in the evolution of organisms over time and the evidence for such change such as the fossil record; special forms of selection such as coevolution, sexual and kin selection; the species concept and speciation mechanisms; and basic concepts in systematics i.e. classification and analysis of relationships among species. GOALS/AIMS The course aims to provide a solid grounding in modern evolutionary biology concepts with reference to examples and evidence derived from a variety of field and laboratory studies encourage students to apply principles of modern evolutionary biology in their experiences of the natural world 52 LEARNING OUTCOMES At the end of this course, students will be able to: Topic: The development of ideas in evolutionary biology 1. Outline the history of evolutionary ideas and define basic concepts in modern evolutionary biology. 2. Define and explain what genetic diversity is, discuss its origins, measurements and importance to the evolutionary process. 3. Outline the main stages in the evolution of the human species and discuss the importance of evolution in understanding human populations, health and disease. Topic: Processes leading to change within populations i.e. microevolution 4. Use the Hardy-Weinberg Principle to infer population stability/instability and use derived Hardy-Weinberg Equations to calculate and predict allelic and genotypic frequencies of genetic disorders and other traits in populations. 5. Use modifications of the Hardy-Weinberg equations to understand, explain and predict phenomena as it applies to Multiple Alleles, Sex Linked Traits and Non-random Mating (Hardy-Weinberg Extensions). 6. Calculate the extent of inbreeding in pedigrees and populations and derive inferences from their results. 7. Identify and discuss the forces that violate Hardy-Weinberg equilibrium (evolutionary forces) and result in evolution. 8. Explain how Natural Selection and Random Genetic Drift (the strong evolutionary forces) modify allelic and genotypic frequencies over time to result in population divergence and speciation (microevolution). 9. Identify and discuss the causes of and the differences between natural selection and random genetic drift. 10. Discuss how the high levels of molecular diversity in nature may be explained by the Neutral Theory of Molecular Evolution. 11. Define what a Molecular Clock is and explain why they can be used to estimate rates of divergence between species. 12. Prove how knowledge of the Neutral Theory of Evolution assists in validation of the existence of Molecular Clocks. 13. Describe how genes control development and discuss the role of development in micro- and macroevolution. Topic: Large scale processes at the geological time scale i.e. macroevolution 14. Explain how fossils are formed and dated and discuss how they contribute to our understanding of past life. 15. Briefly describe major events in the planet’s geological history and how they influenced fossil formation and the fossil record. 16. Outline major divisions of geological time and look at major patterns of fossil record; stasis, punctuation and Cope’s Law 53 Topic: Special forms of selection 17. Distinguish between asexual and sexual reproduction and discuss the advantages and disadvantages of each. 18. Explain the concept of genomic conflict and give examples where genomic conflict may occur. 19. Analyze how natural selection interacts with tradeoffs to design life histories and sex allocation for reproductive success in terms of age and size of organisms on maturation, number of offspring, factors influencing life expectancy, how offspring gender influences parental care. 20. Define “sexual selection” and its two main forms and explain why each acts mainly on males. 21. Outline the logic of the theory of kin selection and explain how it harmonizes altruism with the theory of evolution by natural selection. 22. Define “co-evolution” and discuss some of its major manifestations. 23. Define “mimicry” and its two main forms and discuss how batesian-mimetic complexes may evolve. Topic: The species concept; speciation mechanisms; basic concepts in systematics 24. Define “species” according to the biological species concept and discuss the problems associated with this concept. 25. Outline and distinguish among the scenarios for speciation (allopatry, sympatry, parapatry) showing the importance and mechanisms of reproductive isolation. 26. Discuss the advantages of using phylogenetic characters over phenetic characters in the construction of cladograms and the importance of synapomorphies in the construction of phylogenetic trees. 27. Recognize and interpret phylogenetic relationships when presented with a phylogenic tree. 28. Define and explain the terms “monophyly”, “paraphyly” and “polyphyly” as they relate to phylogenetic trees. 29. Outline the differences between homology and homoplasy and explain how homoplasy can occur and lead to polyphyly. 30. Use comparative genetics, embryology and anatomy (biology) to illustrate evolutionary similarities among various groups of organisms. 31. Outline how comparative biology provides strong evidence for the phenomenon of evolution Topic: General skills 32. Apply principles of the scientific method, evaluate and critique the work of others, think analytically and solve problems; write scientific reports. 54 COURSE ASSESSMENT Students must obtain 50% or more in the sum of marks for all course components below to pass this course (refer to new UWI Grading Scheme below). % Final Description / Assignments Component Grade (see Course Calendar for submission details) 2-hour written exam with multiple choice, short paragraph and Final exam 50% essay questions Coursework 50% Broken down as follows: 10% Tutorials, WACE Performance in tutorial and informal writing exercises and activities 5% Critical Readings Journal Article Critical Review Report 15% Incourse tests 2 tests (each 7.5%) including multiple choice, short answers, short paragraphs or essay 20% Practicals Performance and reporting of 4 practical exercises Please note guidelines and policies for attendance and plagiarism under ‘Additional Information’ and below. ASSIGNMENTS A variety of strategies will be utilized to assess the following Learning Outcomes: Assessment: Learning Outcomes covered: LO 1-32 Tutorials, Writing Across the Curriculum (WACE) (10%): Continuous evaluation of participation and performance and contributions throughout the semester Journal Article Critical Review Report (5%): Written critique LO 32 of a journal paper LO 1-32 Incourse tests (15%): 2 tests (each 7.5%) each 50 minutes duration including multiple choice, short answers, short paragraphs or essay LO 2-5; 6-8; 13-14; 18-22; Practicals (20%): Practical performance and reporting of 4 32 practical exercises during the semester See Course Calendar for Lab and Assignment due dates. EVALUATION Feedback on the course will be obtained informally from students on an ongoing basis by regular interactions and meetings among students, demonstrators, instructors, teaching assistants and the Course Coordinator in practicals and tutorials. Formal feedback will be via election of Class Representatives who sit on the Departmental Student-Staff Liaison Committee meetings held twice during the semester. Class reps will channel both concerns and commendations to the meeting as guided by the Department’s Standard Operating Procedures. 55 Formal evaluation of the entire course will be accomplished via a UWI Course Evaluation questionnaire administered anonymously and confidentially at the end of the semester. All feedback will be considered on an ongoing basis and corrective action or adjustments made or discussed with students promptly or incorporated the following year. TEACHING STRATEGIES The course comprises 24 1-hour lectures, 3 1-hour tutorials and 4 5-hour practical sessions. Tutorials are taken up with collective problem solving with minimal lecture-style talking by the instructor. However, lectures always have a certain tutorial aspect, and the instructor can be expected to pose questions to members of the class in every lecture hour. Lecture and practical content are integrated and there will be continuous reference from one to the other. Students are expected to read and use the resources provided to expand topics summarized in lecture. RESOURCES There are extensive resources available on this topic in the university library and on the internet. In the Main Library relevant textbooks on evolutionary biology mostly have call numbers around QH366 and some are placed on reserve. Selected web-based resources and websites will be posted on myelearning. Students are expected to obtain and read the required textbook which fully covers all the topics in the course. Supporting resources based on the main textbook (videos, self-tests and activities) are also linked from myeLearning. Practical exercises in the field and lab will make use of standard equipment and materials. In addition, we will have access to the department’s significant library of natural-history movies on DVD. READINGS Category A text (available for purchase in UWI Bookshop, student resources available online): Stearns, S. & R. Hoekstra. 2005. Evolution. Oxford University Press. Category B texts (available in UWI Main Library): Allendorf, F.W., G.H Luikart, and S.N. Aitken. 2012. Conservation and the Genetics of Populations. Wiley-Blackwell, 2 ed. Avers, C.J. 1989. Pattern & Process in Evolution. Oxford: Oxford Univ. Press. Others as assigned and/or made available on MyeLearning. Websites: University of California, Berkeley. "Understanding Evolution” http://evolution.berkeley.edu/evolibrary/home.php Stearns, S. & R. Hoekstra. 2005. Evolution. Oxford University Press http://global.oup.com/uk/orc/biosciences/evolution/stearns2e/01student/selftest/ Stephen C. Stearns. Videolectures.net http://videolectures.net/stephen_c_stearns/ 56 COURSE CALENDAR Note that Saturday is regarded as a school day at UWI, and class exercises are sometimes scheduled for the weekend. See last page for detailed Course Calendar. ADDITIONAL INFORMATION: Refer to the Life Sciences Undergraduate Handbook for general guidelines for teaching, assessment, student conduct, essential skills, and support at http://sta.uwi.edu/fst/lifesciences/documents/handbook.pdf . Refer to the General Information and General Regulations in the Faculty Booklet available from http://sta.uwi.edu/resources/documents/facultybooklets/ScienceTechUndergrad.pdf . Attendance: Students are reminded that they must attend and report satisfactorily on a minimum of 75% of the practical sessions and tutorials. Failure to do so may result in debarment from the final examination. Medicals, excuses: Any student who misses a class, test or assignment is advised to consult immediately in person or by email with the TA or Course Coordinator regarding reasons for their absence and make-up options. Absence must be explained in writing within 7 days of the missed session by either a written/emailed excuse sent to the TA or Course Coordinator, and/or a medical certificate submitted to the Health Service Unit and copied to the Departmental Secretary, Life Sciences and the Course Coordinator. Makeups: As a general principle, medicals or other excuses may only excuse a student’s presence at an assigned time. Students must still complete the assigned work (make-up lab report or make-up test) in order to obtain the marks for that item of coursework. The student is responsible for liaising with the Course Coordinator or Teaching Assistant to ensure the assigned make-up is completed. Normally only ONE make-up session will be held. Any student who was inexcusably absent from a practical or test or does not submit a practical report (initial or make-up) will receive 0% for that exercise. Note that a field trip cannot be repeated for a make-up so you should make every effort to attend field trips. Late submission: A penalty at 10% of mark per day late will be applied unless formal approval for late submission is given by the TA or Course Coordinator using the guidelines above for medicals, excuses. Assignment submission: You will be informed whether reports should be in HARD or SOFT copy format. HARD COPY reports should be submitted in the practical session or afterwards into a labelled deposit box for your bench/demonstrator in the outer Zoology office and the student list must be signed. Submissions anywhere else will not be assessed. These will be returned at a time arranged by the TA or demonstrator. We are not responsible for reports left uncollected or submitted inexcusably late. SOFT COPY reports should be submitted as instructed through the relevant assignment link on the BIOL2262 myelearning page. Plagiarism: Students are hereby informed that plagiarism is forbidden and all unsupervised coursework items must be accompanied by a completed Plagiarism Declaration form in order to be assessed. Specific items may require submission through Turnitin on myeLearning. Refer to 57 ‘University Regulations on Plagiarism’ available from http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf Safety in the lab and field: Medical Data and Emergency Contact forms must be completed in the first lab session when safety briefings will be given. Standard laboratory protocols for conduct and dress code are in effect once you are present in a Life Sciences laboratory (Undergraduate Student Handbook section 8). Dress code includes mandatory lab coats, adequate clothing to protect the body, and closed shoes. NO sandals, slippers or open-toed shoes will be allowed in the lab or field. Marks will be deducted for non-adherence to these protocols. Field work: Details are given in section 8 of the Undergraduate Student Handbook http://sta.uwi.edu/fst/lifesciences/documents/handbook.pdf . Field trips are specified on the attached Course Calendar so you can be adequately prepared. You will not be allowed on the field trip if a Medical Data form has not been submitted. Accessibility: If you have a disability and have not disclosed the nature of your disability and the support you need, you are invited to contact the Academic Support/Disabilities Liaison Unit (ASDLU) of The University of the West Indies (UWI), St. Augustine Campus. Phone: 662-2002 extension 83866, 83921, 83923 and 84254; Fax: 662-2002 extension 83922; Email: [email protected] ; http://sta.uwi.edu/asdlu/forms.asp HOW TO STUDY FOR THIS COURSE Attend: You should attend all lectures, tutorials and practicals since satisfactory attendance (>75%) is required by the UWI regulations. Students who attend regularly perform better than those who do not. You should attend to obtain the full value of the lecturer’s presence. Lecturers routinely synthesise and summarise large amounts of information, prioritise, evaluate, discuss and explain this information. They provide the conceptual framework of the course which you must then fill in with details and examples through independent study. Prepare: You should prepare before classes so you can clarify any difficult points with the lecturer or engage in meaningful discussion of any controversial topics. This will enhance and deepen your understanding as well as contribute to course assessment in tutorials and practicals. This is your opportunity for continuous assessment to monitor your progress and to accumulate coursework marks incrementally. Review: You should review learning outcomes for each topic or activity and make sure you understand and can actually achieve those outcomes. If you can’t, please clarify with the lecturer. You must make thorough use of the resources provided (textbooks, myeLearning) from the first week. You should check into myeLearning frequently to review updated materials, assignments, notices and activities. Study: In addition to timetabled contact time, you are expected to devote additional hours of independent study: reviewing class materials and notes, reading/viewing/using resource materials, completing assignments and preparing for assessments. You are strongly encouraged to make your own notes from lectures, textbooks and other source materials throughout the semester to improve your skills of comprehension, organisation, planning and writing. Working in study groups is strongly supported for those who learn best this way as it shares workloads, 58 builds camaraderie and social skills. However, please don’t rote-learn prepared essays and always double check the accuracy of your colleagues’ work. Connect: You are encouraged to interact regularly with staff, even outside of the assigned class times to ensure prompt, satisfactory solution of any problems and to monitor progress. GRADING SYSTEM: The NEW UWI grading scheme effective 2014/15 is given below. See http://www.uwi.edu/gradingpolicy/ for details. GRADE A+ A AB+ B BC+ C F1 F2 F3 QUALITY POINT 4.30 4.00 3.70 3.30 3.00 2.70 2.30 2.00 1.70 1.30 0 % RANGE Grade Definition 90-100 80-89 75-79 70-74 65-69 60-64 55-59 50-54 45-49 40-44 0-39 Exceptional Outstanding Excellent Very good Good Satisfactory Fair Acceptable Unsatisfactory Weak Poor NA/JS/MAK: 01 October 2015 59 BIOL2262 COURSE CALENDAR: WEEK 1 2 3 4 5 6 7 8 9 LECTURES, TESTS 1. Course Introduction. 2. History of ideas in evolutionary biology 3. Basic concepts in evolutionary biology 4. Populations, genes, alleles & genetic variation 5. Hardy-Weinberg equilibrium & its applications 6. Hardy-Weinberg Extensions 7. Violations of Hardy-Weinberg equilibrium: The Weak Evolutionary Forces 8. Violations of Hardy-Weinberg equilibrium: The Strong Evolutionary Forces 9. The Strong Evolutionary Forces: Selection 10. The Strong Evolutionary Forces: Selection 11. Development in evolution 12. In-course Test 1 [7.5%] 13. Key events in evolution 14. The fossil record and life’s history 15. Evolution of sex; Genomic conflict 16. Life history evolution 17. Sexual selection; Kin selection 18. Co-evolution & Mimicry 10 19. Species concept & speciation 20. Molecular Evolution and cladistics 11 21. Phylogeny & systematics 22. Comparative Biology 12 23. Human evolution & evolutionary medicine 24. In-course Test 2 [7.5%] 13 25. Review of Exams/Feedback 26. Course Review NA/JS/MAK: 01 October 2015 PRACTICALS, TUTORIALS , ASSIGNMENTS Briefing on ‘Journal Article Critical Review Report’ WACE 1 [2%] Practical 1: Introduction to HardyWeinberg Equilibrium [5%] Tutorial 1 [2%] Practical 2: Violations of HardyWeinberg Equilibrium: Small populations and its consequences [5%] Tutorial 2 [2%] Practical 3: Fossils Field Trip/Lab [5%] Submit ‘Journal Article Critical Review Report’ [5%] Practical 4: Videos on Evolution [5%] Tutorial 3 [2%] WACE 2 [2%] 60 THE UNIVERSITY OF THE WEST INDIES ST. AUGUSTINE FACULTY OF SCIENCE AND AGRICULTURE DEPARTMENT OF LIFE SCIENCES Course Description COURSE CODE: BIOL2163 COURSE TITLE: Biostatistics NO. OF CREDITS: 3 (23 hours of formal lectures, 6 hours of Tutorials and 20 hours of Practical work solving problems by hand or using statistical software). LEVEL: Undergraduate, Level II PRE-REQUISITES: MATH 1115 or MATH 1125 or AGRI 1003 or Unit I or II CAPE Pure Mathematics or Unit I or II CAPE Applied Maths or Cambridge GCE A’level Mathematics or A/O’ Level Add Maths or equivalent and 9 Credits of Level 1 Life Sciences courses chosen from the following BIOL1262 Living Organisms I, BIOL1263 Living Organisms II, BIOL1364 Genetics I, BIOL1362 Biochemistry I, BIOL1261 Diversity of Organisms and BIOL1061 Cell Biology and Genetics COURSE DESCRIPTION: This course introduces statistical concepts and analytical methods that can be applied to data in the biological, life sciences and environmental sciences. It will teach the basic concepts of experimental design, quantitative analysis of data, and statistical inferences. This course emphasises applications and will help students to statistically evaluate data from biological experiments. Assessment is designed to make students work continuously with the course materials, exploring and critically analysing research and real world data. Assessment will be continuous through assigned problem sheets allowing continuous feedback and guidance on problem solving techniques. COURSE RATIONALE: This Semester I, Level 2 course is part of the core of the Biology degree as well as the Biology and Biochemistry Majors in the Department of Life Sciences. It is also forms part of the core of the B.Sc. Environmental Science and Sustainable Technology. It is designed for students doing their first statistics course, including those with limited mathematical backgrounds. Students should have at least completed an introductory maths course including algebra. It equips students with basic experimental and data management skills needed for most areas of the biological sciences. The student will be introduced to the basics of scientific enquiry, from formulating hypotheses, designing experiments (particularly field experiments), performing appropriate statistical tests to test hypotheses and stating the outcome of the statistical test in a clear concise manner that is comprehensible to a reader who does not have specialist knowledge of the subject matter. 61 INSTRUCTOR INFORMATION: Name of instructor(s): Dr Isaac Dialsingh Office address and phone: Dept. of Mathematics & Statistics, Second Floor, New Wing, Natural Sciences Building Email address: [email protected] Office hours: Thursday 1-3 pm LETTER TO THE STUDENT: Welcome to Biostatistics. In this course you will be introduced to a range of fundamental statistical techniques which equip you with the basics of scientific enquiry. This will include formulating hypotheses, designing experiments (particularly field experiments), performing appropriate statistical tests to test hypotheses and stating the outcome of the statistical test in a clear concise manner that is comprehensible to a reader who does not have specialist knowledge of the subject matter. The course will be taught in blended mode comprising both face-toface and online delivery. You are expected to engage with the materials provided online, in face-to-face lectures and tutorials as well as explore and interact with these to develop the essential skills of critical thinking (clarity, accuracy, relevance, logic, breadth, depth, precision, significance, completeness and fairness). Activities will be diverse and you are strongly encouraged to ask questions, offer new ideas, solve problems and think innovatively to maximise the value of your learning experience. Biostatistics lectures and practical sessions are scheduled during the first semester of year 2 and are sequenced to cover the basic statistical needs of the other advanced courses in the programme. Students will be assigned to one of the practical streams during the practical session in week 1. The Course Manual containing background information and objectives for the course and lecture notes for the Biostatistics section is available online in myeLearning. You should print a copy and bring this for use in the Biostatistics. You should also read the essential texts (listed below). Additional material and notes for lectures, tutorials and practical exercisess will be supplied via myeLearning. Tutorials will be held bi-weekly. Students will be assigned by the Instructor to a tutorial group during the practical session of week 1. The online teaching tool myeLearning will be used extensively during this course for: (i) communication between staff and students (ii) official posting of important notices (iii) providing of course details, lecture objectives, practical guides, data for practical exercises, tutorial briefings; this year it is the only source of the full course manual. You are expected to become proficient in the use of myeLearning and check in on a regular basis (at least once per week, well before your practical class). CONTENT: The following Topics will be covered: Introduction to Biostatistics Course Overview Types of Data Experimental Design Describing, Exploring and Comparing Data Frequency distributions Visualizing Data Measures of Central Tendency Measures of Variation Probability Theory and Distributions The Addition Rule The Multiplication Rule 62 Conditional Probability The Standard Normal Distribution The Binomial Probability Distribution The Poisson Probability Distribution Estimates and Sample sizes with One Sample Estimating a Population Proportion Estimating a Population Mean Estimating a Population Variance Hypothesis Testing with One Sample Basics of Hypothesis Testing Testing a claim about a Proportion Testing a claim about a Mean, Standard Deviation or Variance Inferences from Two Samples Inferences about Two Proportions Inferences about Two Means Correlation and Regression Correlation Regression Multinomial Experiments and Goodness of Fit Goodness of Fit Analysis of Variance One-Way ANOVA Two-Way ANOVA Nonparametric Statistics Sign Test Wilcoxon Test Kruskal-Wallis Test Rank Correlation GOALS/AIMS: This course aims to: Review the range of routine statistical tests and provide the student with a basic theoretical understanding. Develop confidence in the students in deciding which statistical test to use in a particular situation. Develop competence in the student in carrying out the statistical test accurately by hand first before using statistical software LEARNING OUTCOMES* Upon completion of the course, students will be able to: 63 interpret statistical data Express the results of statistical evaluation in a clear and concise manner, comprehensible to a reader who does not have specialist knowledge of the subject matter. Recognize and give examples of different types of data arising in life sciences disciplines Interpret differences in data distributions via visual displays Calculate standard normal scores and resulting probabilities Calculate and interpret confidence intervals for population means and proportions Interpret and explain a p-value Perform a two-sample t-test and interpret the results; calculate a 95% confidence interval for the difference in population means Select an appropriate test for comparing two populations on a continuous measure, when the two sample t-test is not appropriate interpret results from Analysis of Variance (ANOVA), a technique used to compare means amongst more than two independent populations Choose an appropriate method for comparing proportions between two groups; construct a 95% confidence interval for the difference in population proportions interpret relative risks and odds ratios when comparing two populations Use the MINITAB or other statistical software to o Perform two sample comparisons of means and create confidence intervals for the population mean differences o Compare proportions amongst two independent populations o Interpret output from statistical software packages related to the various estimation and hypothesis testing procedures covered in the course procedures. COURSE ASSESSMENT Assessment will be based on a student’s final mark from the coursework components below. % Final Description Component Grade 2-hour written exam with statistical exercises. NonFinal exam 50% programmable calculators will be required. 2 tests (each 5%) based on short answer or multiple choice In-course tests 10% questions Tutorials, online Oral face-to-face contribution in interactive discussions. 10% activities Online quizzes. Statistics research 4-5 page data analysis written report targeted at a 10% report statistically naive reader. Practical Performance and reporting of problem sheets in 5 practical 20% Exercises exercises ASSIGNMENTS 2 coursework tests (5% each): 50 minutes duration including short answer or multiple choice questions Tutorials, online activities (10%): Continuous evaluation of participation and performance and contributions throughout the semester (discussions, wiki/ glossary, blogs). Reading/ research project (10%): On one occasion during the last month of the course, the instructor will designate a data analysis problem requiring a 3-5 page written report from the students. The report should be 64 written for a statistically naive reader. Practicals (20%): Practical performance and written lab report at 5 practical exercises during the semester. 1. Biostatistics 1: visualizing data 2. Biostatistics 2: analysis of frequency data 3. Biostatistics 3: regression & correlation 4. Biostatistics 4: analysis of two groups 5. Biostatistics 5: analysis of Rank data EVALUATION Feedback on the course will be obtained informally from students on an ongoing basis by regular interactions and meetings among students, demonstrators, teaching assistants and the Course Coordinator in practicals and tutorials. Formal feedback will be via election of Class Representatives who sit on the Departmental Student-Staff Liaison Committee meetings held twice during the semester. Class reps will channel both concerns and commendations to the meeting as guided by the Department’s Standard Operating Procedures. Formal evaluation of the entire course will be accomplished via a UWI Course Evaluation questionnaire administered anonymously and confidentially at the end of the semester. All feedback will be considered on an ongoing basis and corrective action or adjustments made or discussed with students promptly or incorporated the following year. TEACHING STRATEGIES Contact hours (38 credit hours): Lectures: 23 h Tutorials: 6 h Practicals: 10 h (5x4 h = 20 h) Lectures: Lectures will provide valuable synthesis and evaluation of the growing body of available information, update current issues and events, and prioritise content relevant to course assessment. Practicals: Fortnightly practical sessions will provide hands on experience for students to gain skills required for solving basic statistical problems by hand using a calculator as necessary. Once basic competency is mastered students so that students understand what they are doing, they will be introduced to more automated methods using statistical software. Participation and performance in the practicals will be assessed as well as reports based on the practical activities. Tutorials: Tutorials will cover course topics in a highly interactive format using a variety of collaborative active learning techniques. In addition to clarifying details of course content, specific transferrable skills will be addressed e.g. performing calculations, report writing and summarisation for the statistical naive reader, critiquing online and face-to-face. myelearning: The online teaching tool, myeLearning, will be used extensively during this course for communication among students and staff (email, discussions) official posting of important notices (coursework assessment notices, instructions, and in-course results) provision of course details, lecture notes, practical guides, tutorial briefings provision of recommended resource materials and links to resources on specific websites self-test quizzes, questionnaires, surveys interactive activities such as discussions, wikis, glossaries, blogs RESOURCES Most resources are available myeLearning including 65 Lecture presentations including learning objectives, summaries, recommended readings and links to papers, articles and websites with interactive resources and videos for those who prefer to learn using these modalities ‘General Resources’ provides direct links to general websites, articles and publications relevant to the whole course Tutorials may have specific supporting resources particularly on skills development READINGS Essential and Recommended texts are in the Reserve Section, UWI Main Library or via links in the course outline and myelearning. Essential Texts 1. Barnard, C., Gilbert, F. & McGregor, P. 2007. Asking Questions in Biology: a Guide to Hypothesistesting, Experimental Design and Presentation in Practical Work and Research Projects. 3rd edition. Harlow: Pearson Education. 2. Department of Life Sciences. 2013/14. BIOLxxxx Biostatistics. Course Manual. This will only be available online in myelearning, and students should print their own copies. Recommended Texts Any of the texts below should be used for supplementing the text books, following up on lectures, and for research for practical reports. 1. Triola M.M. and Triola MF. 2006. Biostatistics for the Biological and Health Sciences 2006. Pearson/ Addison Wesley, Boston. (QH323.5.T75 2006) 2. Rosner B. 2010. Fundamentals of Biostatistics, 7th ed. Duxbury Press, Boston (QH323.5.R822) 3. Zar, J.H. 1984. Biostatistical Analysis. 2nd ed. Prentice-Hall, N.J. COURSE CALENDAR WEEK 1. 2. 3. 4. 5. 6. 7. 8. LECTURES 27. Course Overview 28. Experimental Design 29. Descriptive Statistics 30. Descriptive Statistics 31. Introduction to Probability 32. Probability distributions 33. Estimating a population proportion 34. Estimating a population mean and variance 35. Basics of hypothesis testing 36. Testing claims about Proportions and Means 37. Inferences about Two Proportions 38. Inferences about Two Means 39. Correlation 40. Regression 41. Multinomial Experiments 42. Goodness of Fit PRACTICALS TUTORIALS Practical 1: Assignments Tutorial 1 Practical 2: Tutorial 2 Deadline online activity 1 Practical 3: Tutorial 3 Test 1 Practical 4: Tutorial 4 Deadline online activity 2 66 9. 10. 11. 12. 43. One-Way ANOVA 44. Two-Way ANOVA 45. Non-Parametric Statistics: The Sign Test 46. Non-Parametric Statistics: The Wilcoxon Test 47. Non-Parametric Statistics: The Kruskal-Wallis Test 48. Non-Parametric Statistics: Rank Correlation 49. Course Review Practical 5: Tutorial 5 Tutorial 6 Submit final research report Test 2 13. ADDITIONAL INFORMATION HOW TO STUDY FOR THIS COURSE Students should attend all lectures, tutorials, and practicals. The lectures are designed to cover the most important points in each subject area, in the opinion of the lecturer, and are condensed from a much greater volume of material. Students who do not attend lectures miss the benefit of this work by the lecturer, and have to review the textbooks themselves. Although less satisfactory than attending lectures, independent study has been made easier by detailed learning objectives cross-referenced to sections of the textbook. Practical instructions are provided on myelearning well before the start of the class, and should be read before attending the class to make full benefit of the class time. Tutorials are designed to help students with aspects that have proved difficult in the past, rather than providing new material. GRADING SYSTEM Students will be graded fairly on work that they have done themselves. Incidents of cheating (this includes plagiarism) will be dealt with according to the UWI regulations1. The Grade Point Average (GPA) system now in use in the UWI is generally used to determine your class of honours when you graduate. Since BIOL 2xxx is a Level II course, your GPA in this course is used to determine your class of honours. Your ability to receive GATE funding in the next academic year is riding on your GPA – should you have a GPA of less than 1.0 at the end of the academic year, GATE will be discontinued, and you will have to pay your own tuition. You will only be able to reapply for GATE after you raise your GPA to 1.0 or greater. To calculate your GPA, find the Quality Points that correspond to the grade in each course. Multiply the number of quality points by the credit weighting for the course, add up all these numbers, then divide by the number of credits. See below for an example. Let’s say you have taken the following courses: Course Credits Grade Quality Points CHEM 1XX0 3 B+ 3.3 CHEM 1XX1 3 A 4.0 MATH 1115 3 B 3.0 1 Examination Regulations for First Degrees, Associate Degrees, Diplomas and Certificates. Section (B) Cheating. Regulations 96– 102; http://sta.uwi.edu/resources/documents/Exam_and_GPA_regulations.pdf. University Regulations on Plagiarism (First Degrees, Diplomas and Certificates). http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf. 67 Then your GPA calculation would look like this: Course Calculation CHEM Credits x Quality Pts. = 3 x 3.3 = 1XX0 CHEM Credits x Quality Pts. = 3 x 4.0 = 1XX1 MATH Credits x Quality Pts. = 3 x 3.0 = 1115 Total C x QP = 9.9 + 12.0 + 9.0 = Total # of credits = 3+3+3= GPA = 30.9 / 9 = Total 9.9 12.0 9.0 30.9 9 3.43 The performance criteria that is expected of you to obtain each grade is outlined in the following table. This information should give you some idea of the level at which you should be working, to achieve the grades you wish to get. And should your grade not quite match up to your own expectations, take another look at the table, and see where you may have gone astray. Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 68 THE UNIVERSITY OF THE WEST INDIES, ST. AUGUSTINE FACULTY OF SCIENCE & TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE DOCUMENTATION COURSE CODE: BIOL2164 COURSE TITLE: Principles of Molecular Biology NO. OF CREDITS: 3 LEVEL: II SEMESTER: II PRE-REQUISITE(S): Either BIOL1362 Biochemistry I and BIOL1364 Genetics I or BIOL1061 Cell Biology and Genetics ANTI-REQUISITE(S): BIOL3061 Molecular Biology COURSE DESCRIPTION This course provides an introduction to recombinant DNA technology, R-DNA cloning, and applications of RDNA technology. It examines the importance of restriction endonucleases in gene cloning, methods of construction of vectors and their applications in developing gene libraries. The methods of screening and enrichment of libraries are also examined. The principles of the Polymerase Chain Reaction (PCR) and its applications including paternity testing and fingerprinting, are also discussed. The principles of sequencing and the expansion of next-generation sequencing techniques are examined. Approaches to locating genes, including map-based gene isolation, and methods of regulating gene expression, including RNAi, co-suppression, and overexpression are discussed using detailed examples. All techniques are further examined under general and holistic approaches to studying the genome, through forward and reverse genetics approaches, functional genomics, transcriptomics, proteomics and metabolomics. In this course, the theoretical prinicples discussed during the lectures are reinforced by practical activites that aid in student learning anf understanding. As this is a practical – based course, activities in the lab, such as quizzes, lab reports and discussions are all assessed. COURSE RATIONALE This course is a techniques-based course that seeks to provide students with the requisite knowledge that is the basis of many of the applied experimental techniques in biology and biotechnology. The student, upon completion of this course, should have a comprehensive understanding and practical expertise in basic molecular biology techniques including DNA extraction, Restriction Enzyme digestion, Hybridization techniques and PCR. This foundation is important for the understanding of more advanced techniques and their application in many biologyrelated fields. This course is a core course in the Biology degree and directly services the Biotechnology option listed in the program. 69 INSTRUCTOR INFORMATION Name of course coordinator: Dr. Georgette Briggs Office address and phone: Biochemistry Office, Floor 2, Old wing, Natural Sciences Building Email address: [email protected] Office hours: Mondays and Fridays 2:00-3:00 pm Preferred method of contact: Email Communication policy: Students should use their UWI email account for communication and can expect a response within 48 hours (Monday to Saturday). COURSE GOALS: On completion of this course, students should have: An appreciation of the role of R-DNA technology in the advancement of scientific research An understanding of the theory that underlies the basic molecular biology techniques An appreciation of the applications of molecular biology techniques in addressing real-life situations Developed laboratory skills in the molecular biology techniques covered The ability to interpret the outcomes of experimental scenarios Acquired the knowledge to aid in evaluating the applicability of molecular biology techniques to specific experimental aims Acquired specific analytical skills with respect to problems in molecular biology CONTENT The Principles of Molecular Biology focuses on the techniques that underlie many of the experiments used many biology disciplines. The course begins with a review of each of the biological molecules (DNA, RNA and proteins) in relation to the Central Dogma, and how they can be detected in the lab. This is followed by an understanding of the application of these detection techniques to real-world situations and further, how DNA can be amplified via PCR. The second module addresses the manipulation of the molecules in the form of R-DNA technology. The importance of hybridization methods and PCR in gene cloning and principles of gene and genome sequencing. The third module addresses the use of methods to control and regulate gene expression, allowing the examination of various processes at the cell and organismal levels. The general topics include: - Principles of Recombinant DNA technology and gene cloning - Hybridization, PCR and its applications - Vectors and gene cloning strategies - Construction of genomic libraries - Gene identification and mapping - Control and regulation of gene expression - Forward and Reverse genetics - Transcriptomics, proteomics and metabolomics - Bioinformatics methods and tools 70 LEARNING OUTCOMES Students completing this course should be able to: Explain the applicability of R-DNA technology to biological research and discovery Explain, illustrate and interpret the experimental steps of the molecular biological techniques covered Demonstrate efficient lab skills in the experimental techniques covered Apply molecular biological techniques to the elucidation of real-life scenarios Make critical evaluations of the applicability of molecular biological techniques to experimental aims Communicate ideas and critically evaluate hypothetical scenarios COURSE ASSESSMENT As stated in the DLS hanbook, students must pass (40%) the practical component of the course to be allowed to sit the final exam. As previously indicated, this is a techniques-based course and therefore 22% of the course marks is assigned to content knowledge and practical skill in this area. The three in-course tests, examines content knowledge in the three modules of the course. In-course test 1: In-course test 2: In-course test 3: Practical reports/quizzes Practical (lab etiquette/participation) Final examination (2 hours) 7% 7% 7% 27% 2% 50 % EVALUATION The elected Class Representative and/or Deputy will attend meetings with the course teacher(s) organized at every 4th week, and present feedback from the students attending that course or stream. This feedback is normally provided both orally and in written form for transmission to the lecturer. Apart from that the representatives will be attending the Liaison Committee meeting, and give their feedbacks to the committee. Students may comment on any aspect of the course or facilities. Students will be encouraged to submit their feedback (oral/written) during tutorials directly to the course teacher and appropriate actions will be taken by the teacher then and there. Results of in-course tests, quiz and other course assignments will be analyzed and presented in the class. This will help students to check their progress constantly and also helps the instructor to identify the weak areas and thereby could alert and advise students individually to alter their approach of study and completing the work. The final reflective feedback and comments about the entire course and teaching will be collected on the last day of the course. This will be saved for analysis and utilized as a base for improvement for the next offering in the following year. TEACHING STRATEGIES The instruction for this course would comprise of 2 lectures per week (50 mins each), and one practical (3 hr) practical session every 2 weeks. Additionally, instructional videos, tutorials and practical labs (wet and virtual labs) would be utilized to support the teaching. This course is also my e-learning supported, and many other resources are readily available for the students. In this course, the practical component is essential in reinforcing the content taught in the lectures. Students in the practical sessions, work in groups of 4 during these hands-on 71 sessions, for which experimental planning (pre-labs) and small group discussions are required. This format therefore addresses a range of learning styles, as outlined in the DLS undergraduate handbook. RESOURCES Recommended reading: Primrose, S.B. and Twyman, R.M. 2006. Principles of Gene Manipulation and Genomics 7th Edn. Blackwell Publishing Ltd. Lodish, H., Berk, A., et al. 2007. Molecular Cell Biology, 6th edn. Freeman. Weaver, R.F. 2006. Molecular Biology, 4th edn. McGraw Hill Higher Education. Glick, B.R., Pasternak, J.J. 2010. Molecular Biotechnology: Principles and Applications of Recombinant DNA. ASM Press Other resources: The course instructor will upload in “my e-learning”, periodically (at the beginning and during the course), important research articles, review papers, book chapters and other related study materials. Students are expected to read them as these were considered and included as portions of the syllabus and covered during examinations. COURSE CALENDAR Week Lecture Topic Wk1 Course Introduction and Overview/Central Dogma Wk1 Wk2 Wk2 Wk3 Restriction Endonucleases Principles of detection via hybridization Principles of detection via hybridization Amplyfying DNA for detection PCR Wk3 Wk4 Wk4 Wk5 Amplyfying DNA for detection PCR Principles of sequencing CLASS TUTORIAL IN COURSE I: wks 1 to 4 Wk5 Wk6 Wk6 Wk7 Introduction to R-DNA technology Vectors and cloning strategies Vectors and cloning strategies Genomic Libraries: Construction and screening Wk7 Genomic Libraries: enrichment Lab Schedule Lab Safety Talk, Lab tour, lab apparatuses and equipment Lab#1:DNA extraction and RE digest Lab#2: Cloning and transformation Lab#3: Virtual labs (Southern, Northern, Western & PCRs) setting-up transfer 72 Hybridize a prepared membrane Wk8 Wk8 Wk9 Locating genes: shot gunning, map-based gene isolation CLASS TUTORIAL IN COURSE II: wks 5 to 7 Lab#4 Washes and detection Wk9 Control of gene expression: silencing, anitsense and RNAi Setting-up a PCR (mock sample) Wk10 Control of gene expression: silencing, anitsense and RNAi Wk10 Forward/Reverse Genetics Wk11 Transcriptomics, proteomics and metabolomics Lab #5: Gene databases Wk11 Transcriptomics, proteomics and metabolomics Wk12 CLASS TUTORIAL / course review Wk12 CLASS TUTORIAL/course review ADDITIONAL INFORMATION Attendance Attendance in the in-course exams and the quizzes is mandatory. Any student who misses an in-course exam or a quiz is advised to consult immediately in person or by email with the course instructor regarding their make-up options. Absence must be accompanied by a written excuse or medical submitted to the Main office, Life Sciences within 7 days of the missed session. Any student who was inexcusably absent or who does not write an in-course exam or a quiz will receive 0% for that exercise. How to study for this course Students are encouraged to work together in small cohesive groups as much as possible to go through the course content. As we go through the various topics, students should attempt to answer all the sample questions placed on my e-learning and discuss the answers amongst themselves. All comments, questions and concerns provided on a particular topic will be addressed during the discussion segments of laboratories and during class time. Your Departmental course textbook on my e-learning contains all the topics to be taught and the textbook content is aligned similarly as the lectures; please read the textbook. Use the responses and comments for your quizzes and in-course examinations as a guide to answering the questions properly. There are several past paper questions in the library and students are encouraged to practice these questions. 73 Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 74 THE UNIVERSITY OF THE WEST INDIES ST AUGUSTINE FACULTY OF SCIENCE AND TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE OUTLINE Course title: Genetics II Course Code: BIOL2165 Credits: 3 Level: TWO Semester: ONE Pre-requisites: BIOL1364 Genetics I or BIOL1061 Cell Biology and Genetics and 6 credits from among the following courses: BIOL1262 Living Organisms I, BIOL1263 Living Organisms II, BIOL1362 Biochemistry I or BIOL1261 Diversity of Organisms. Anti-requisite: BIOL2162 Advanced Genetics. Course Description: Genetics II is a core course for the Biology programme in the Department of Life Sciences. The major topics of the course are cytogenetics (including epigenetics and developmental genetics), prokaryotic/ viral genetics, and molecular genetics (including genomics). Cytogenetics explores chromosomal macromutations (chromosomal deletions, duplications, inversions and translocations) and their associated cytogenetic effects on plants and animals. Epigenetics and developmental genetics is a new area of study that explains the environmental influence on chromatin dynamics, DNA methylation, development and ultimately on inheritance. An introductory treatment of developmental genetics is also given to understand master control genes (homeotic genes) that regulate a cascade of genes that control development. Prokaryotic/ viral genetics provides insights into prokaryotic/ viral reproduction, recombination; genetic complementation, mapping; and genetic regulation. Molecular genetics provides the fundamental basis for the understanding of Molecular Biology and as such deals with DNA replication, transcription, translation and controls. Genomics provides an insight into where genetics is evolving (including an introduction to applications). Assumed Knowledge: Students should be skilled in the basic principles of genetics including the nuclear genome; the cell cycle; Mendelian genetics and extensions; and gene mapping. In addition, students should have mastered the basic concepts of Biochemistry including the structure and function of biomolecules. Course organization The course is divided into three sections: cytogenetics (40%), prokaryotic/ viral genetics (20%), and molecular genetics (40%). 75 Cytogenetics will be taught in the first 5 weeks in the following order: Chromosomal macromutations (their detection-both cytological and genetic, and the significance of each type of macromutation (benefits, associated conditions and diseases, evolutionary significance) – deletions, duplications (including multigene families and homeotic genes), inversions, translocations, autopolyploids, allopolyploids, aneuploids; epigenetics; and homeobox genes. Prokaryotic genetics will be taught in weeks 6 and 7 in the following order: Importance of prokaryotic genetics, comparison of prokaryotic and eukaryotic genomes, recombination in prokaryotes (transformation, transduction and conjugation), conjugation mapping, recombination and complementation tests (their uses and limitations), and transposition; viral genetics- bacteriophage genetics (mating, recombination, genetic mapping). Molecular genetics focusses mainly on the eukaryotic genome and will be taught from week-8 to week-12 in the following order: DNA replication, transcription, translation, their associated processes, end products and their regulation; the genetic code; and genomics (including the evolution of today’s concept of a gene and a gene locus). Purpose of the course Genetics II (BIOL 2XXX) aims to build on the foundation of basic principles in Genetics through the delivery of advanced topics spanning three major topics: Cytogenetics, Prokaryotic Genetics and Molecular Genetics. This course will serve as a core requirement for the fulfillment of the Biology major in the Department of Life Sciences, University of the West Indies. Furthermore, this course serves as the feeder course to Microbiology, Microbial Biotechnology, Molecular Biology, Plant Biotechnology, Animal Biotechnology, and Crop Improvement as well as several M. Phil. and Ph.D. programmes offered in these aforementioned areas in the Department of Life Sciences. Careers which demand an advanced knowledge of Genetics include Plant Breeders, Conservation Geneticists, Biotechnologists and Genetic Engineers as well as teachers of Biology at the secondary and tertiary school levels. Lecturer information Dr. Winston Elibox, Room 312, 3rd Floor Natural Science Building, Tel: 6622002 Ext. 83108; E-mail: [email protected] Office hours: Monday – Friday: 12:00 p.m. – 4:00 p.m. Communication Policy: I prefer communication via e-mail using your UWI email account. Letter to the students Dear Students, I wish you a warm welcome to BIOL2XXX- Genetics II. I am excited that you have decided to pursue this course which will build on the concepts that you have learned in Genetics I (BIOL1364) and specifically provide you with a sound knowledge in advanced topics in genetics such as chromosomal macromutations; epigenetics and developmental genetics, prokaryotic/ viral genetics and molecular genetics. Chromosomal aberrations such as 76 deletions, duplications, inversions and translocations can have profound effects on plants and animals including several genetic diseases of humans. All the multiple gene families that are responsible for things like immunity, expression of haemoglobin, and body architecture are a result of duplications. Furthermore most of our important cash crops are derived by duplication of identical or non-identical genomes (polyploids). We can use translocation heterozygotes to control insect pests. This course will further show you that although twins have the same genetic make-up, because of their life history, they are epigenetically different. The area of developmental genetics will show you how a simple egg can become an adult of its species based on pre-programmed processes encoded by master control genes (homeotic genes). You will see why prokaryotes are able to recombine and form new strains that can pose potential threats to us and our food sources. Finally, you will gain sound knowledge on how DNA is maintained from generation to generation, how it is replicated, how and when it is transcribed and how the transcribed mRNA is eventually translated to form the polypeptides/ proteins that carry out the functions of a body. The course is very modern and gives a brief introduction to all the modern tools of genetics that are currently being used in the fields of biotechnology, molecular biology, crop improvement, conservation and medicine. Hence this course serves as a foundation course for many of your advanced courses as well as many of your future M. Phil. and Ph.D. programmes. The course is myelearning supported and several resources including the course handbook and other suggested readings can be accessed at your convenience. Several thought provoking questions will be posted in the discussion forum of myelearning as we go through the course content and you are encouraged to participate. Please check your timetables and pay particular attention to lecture times, quizzes, incourse examinations and tutorials. I wish you a fruitful semester and I look forward to working with all of you. Sincerely, Dr. Winston Elibox Lecturer Course Content Topic 1: Cytogenetics Regulation of gene expression at the chromosomal level Specialized forms of chromosomes Chromosomal mutations, changes in chromosome structure: The origins, inheritance, evolutionary significance and diagnosis of chromosomal deletions, duplications (including multiple gene families), inversions and translocations Chromosomal mutations, changes in chromosome number: The origins, inheritance, evolutionary significance and diagnosis of euploidy (autopolyploid and allopolyploid), and aneuploidy Epigenetics (heritable changes in gene function without a change in DNA sequence in chromosomes): DNA methylation results in different phenotypes in genetically identical organisms; types of epigenetic imprinting; role of epigenetic markers in the remodeling of chromatin; inheritance of epigenetic imprints; role of epigenetics in establishing and maintaining cell identity; epigenetic switching 77 Homeobox genes (master control genes): Importance of homeobox genes in developmental processes in multicellular organisms; identifying homeobox genes; phylogenetic distribution of homeotic genes; regulation of homeotic gene complexes Topic 2: Prokaryotic/ viral genetics Prokaryotic genome structure and organization Genetic recombination in prokaryotes: conjugation, transduction and transformation Mechanism of each type of genetic recombination Creating genetic maps for bacterial chromosomes using conjugation Transposition (Transposons – mobile segments that cannot exist independent of a replicon): structure of the three types of transposons- insertion sequence elements, composite and non-composite; mechanism of transposition; significance of transposons in multiple drug resistance in bacteria Gene fine structure analysis: recombination and complementation testing in bacteria; recombination and complementation spot test in bacteriophages; Benzer’s deletion mapping technique in bacteriophages Topic 3: Molecular genetics Molecular organization of the eukaryotic genome Genomics- evolution of the modern concept of a gene DNA replication in viruses, prokaryotes and eukaryotes DNA transcription in prokaryotes and eukaryotes Post transcriptional modifications and mRNA processing Regulation of gene expression in prokaryotes (negative, positive and attenuation) Regulation of gene expression in viruses (bacteriophages ) Regulation of genes in eukaryotes (facultative and condensed chromatin, position effects, methylation), transcriptional control, post-transcriptional control, translational control and post-translational control. The nature of the genetic code, degeneracy of the genetic code DNA translation: structure and function of the ribosomes, role of tRNA, steps in DNA translation Post translational modifications Course Goals At the end of this course, students should have: Acquired knowledge of chromosomal macromutations; their importance, detection; associated disorders and evolutionary significance; and possible applications in medicine and agriculture. Acquired knowledge in epigenetics and developmental genetics; their importance in differentiation, dedifferentiation, development and maintenance of cell types in various tissues and organs. Acquired knowledge of prokaryotic/ viral genetics, the importance of studying their genetics; and their use in the production of high density genetic maps. Acquired knowledge of the various steps that result in a gene in the DNA of a chromosome being replicated, transcribed and translated into a polypeptide (protein) and the degeneracy of the genetic code; genomics and the evolution of the concept of a gene and locus and the methods of gene regulation. General objectives The course aims at providing students with the knowledge, comprehension and application of advanced genetic principles in the areas of cytogenetics, prokaryotic/ viral genetics and molecular genetics through lectures, 78 discussions, assignments and tutorials. More specifically, the course will deal with the organization, structure, function and regulation of the genetic material of prokaryotes and eukaryotes at the molecular and gross levels. An introduction to the concept of the gene and methodologies that have led to the advancement of knowledge on gene control will also be presented. To assess learning, three incourse exams during weeks 5, 8 and 12; three quizzes (weeks 2, 6 and 10) based on three assignments; and one worksheet will be given and graded. The course is myelearning supported and your manual contains specific course objectives as well as lecture and assignment outlines and more. Students’ answers to the course questions posted on myelearning will also be used to assess learning. Learning outcomes: At the end of this course, students will be able to: Topic 1: Cytogenetics Introduction to Chromosomes & Chromatin i. ii. iii. iv. v. vi. vii. viii. ix. Define cytogenetics, state the chromosome theory of inheritance and explain how it led to the evolution of cytogenetics. Describe chromosome structure at the macro and ultra-levels and define the unineme model. Describe the procedure by which karyotyping is performed and state its importance in determining basic chromosome number, size, shape, chromosomal macromutations, ploidy levels. Differentiate between heterochromatin and euchromatin in a chromosome. Define and distinguish between the different types of heterochromatin: facultative, constitutive & condensed. Describe the staining methods used to visualize the forms of chromatin making up chromosomes (Feulgen staining, G-banding, FISH, Q-banding, R-banding). Discuss the role of chromatin in regulating gene expression at the chromosomal level with a clear definition of the phenomenon of position effects. Define and describe polytene chromosomes & lampbrush chromosomes as two examples of specialized forms of chromosomes. Explain the significance of polytene and lampbrush chromosomes and their role in tissue specific amplification of gene expression. Changes in chromosomal structure Chromosomal Macromutations: Deletions i. ii. Define deletions. Describe and distinguish between the different types of chromosomal deletions with the aid of appropriate diagrams. iii. Describe the cytological and genetic methods used to detect chromosomal deletions. iv. Explain the consequences of deletions and describe the inheritance of deletions using specific examples. v. Explain the evolutionary significance of chromosomal deletions. Chromosomal Macromutations: Duplications i. Define duplications. 79 ii. Describe and distinguish between the different types of chromosomal duplications with the aid of appropriate diagrams. iii. Describe the cytological and genetic methods used to detect chromosomal duplications. iv. Explain the consequences of duplications and describe the inheritance of duplications using specific examples. v. Explain the evolutionary significance of chromosomal duplications using specific examples. vi. Define multigene family (a form of chromosomal duplication) and briefly describe specific examples of these (immune system super-family, collagen gene family, cytochrome P450 gene family). vii. Describe how multigene families arise and evolve to generate functional diversity, environmental flexibility & developmental flexibility using the immune system super family as an example. viii. Define the term, pseudogene, as it relates to gene families and explain how they arise. Chromosomal Macromutations: Inversions i. ii. Define inversions. Describe and distinguish between paracentric and pericentric inversions with the aid of appropriate diagrams. iii. Describe the cytological and genetic methods used to detect chromosomal inversions. iv. Explain and illustrate the meiotic consequences of crossovers within inversion loops for pericentric and paracentric inversion heterozygotes. v. Describe and explain the phenotypic effects associated with chromosomal inversions using specific examples. vi. Define the term apparent crossover suppression as it relates to chromosomal inversions and explain how it differs from actual crossover suppression associated with other chromosomal macromutations such as deletions. vii. Explain the evolutionary significance of chromosomal inversions using specific examples. Chromosomal Macromutations: Translocations i. ii. iii. iv. Define translocations. Describe and illustrate the different types of chromosomal translocations. Describe the cytological and genetic indicators used to detect chromosomal translocations. Describe and distinguish between alternate, adjacent-1 & adjacent-2 segregation patterns observed in meiosis for translocation heterozygotes. v. Explain and illustrate the meiotic consequences of translocations in heterozygotes. vi. Describe and explain the phenotypic effects associated with chromosomal translocations using specific examples (Robertsonian translocation; Down syndrome, familial Down syndrome). vii. Explain the application of translocation heterozygotes in pest control. viii. Explain the evolutionary significance of chromosomal translocations using specific examples. Changes in chromosome number Polyploidy i. ii. iii. Define and distinguish between the basic chromosome number (x), the haploid number (n) and the total chromosome number. Define the term polyploidy. Define and distinguish between the two main forms of ploidy: euploidy & aneuploidy. 80 iv. v. vi. vii. viii. ix. Define and distinguish between autopolyploidy and allopolyploidy as forms of euploidy. Explain how non-disjunction in mitosis and meiosis can lead to the formation of autopolyploids. Explain how autopolyploidy can be induced artificially. Describe the inheritance of autopolyploidy and explain the sterility observed in triploids. Describe the polyploidy series in Musa spp. (autopolyploids). Explain and illustrate how interspecific hybridization followed by duplication and diplodization can lead to the formation of fertile allopolyploids. x. Discuss the role of allopolyploidy in evolution using specific examples. xi. Define and distinguish between the two forms of aneuploidy: hypoploidy & hyperploidy Heritable changes in cellular expression that occur without a change in the DNA sequence Epigenetics & Chromatin Dynamics i. ii. Define the terms epigenetics and imprinting. Describe the process of DNA methylation and explain its epigenetic effect on chromatin remodeling and gene expression. iii. Identify methylation, phosphorylation & acetylation as epigenetic markers and describe the mechanism by which these epigenetic patterns are established on histone proteins in the remodeling of chromatin. iv. Explain how epigenetic imprints are inherited. v. Explain the role of epigenetics in establishing and maintaining cell identity. vi. Define the term epigenetic switching and differentiate between this process in plants and animals. vii. Explain the significance of epigenetics in the development of cancer in humans. Master control genes that regulate a cascade of other genes and control development in multicellular organisms Homeotic genes- master control genes i. ii. iii. iv. v. vi. vii. Define homeotic genes as being gene families that share a common DNA sequence element (homeobox) in multicellular organisms Explain the function of homeotic genes- master control genes (switch genes) that specify developmental patterns (regulation) by turning different processes of cellular differentiation on or off. Discuss how homeotic genes begin regulation from the very early stages of embryogenesis. Discuss the mechanism of function of homeotic genes in Drosophila melanogaster. Discuss the origin and phylogenetic distribution of homeotic genes. Describe how homeotic genes are identified. Discuss how mutations in homeotic gene families such as the Hox gene can affect rib and limb development in humans. Topic 2: Prokaryotic/ viral genetics Genetic Recombination in Bacteria - Conjugation i. Describe the main distinguishing features between eukaryotic and prokaryotic genomes with particular regard to structure, organization, inheritance/transmission & recombination. 81 ii. Define the term conjugation as it relates to bacterial recombination and state the requirements for successful genetic transfer via bacterial conjugation. iii. Describe the U-tube experiment that provided evidence for conjugation as a mechanism of gene transfer between bacterial cells, eventually leading to genetic recombination. iv. Describe the functions of the F-factor and its role in bacterial conjugation. v. Define and distinguish between donor (F+-strain) and recipient cells (F--strain). vi. Define the terms plasmid and episome and explain how they are different from each other. vii. Compare and contrast bacterial conjugation involving F+, Hfr & Lfr strains giving detailed descriptions of the mechanism of transfer from donor to recipient. viii. Define the term F’-factor and explain how it is formed. ix. Define the term F-mediated sexduction and explain how it leads to the formation of merozygotes. x. Differentiate between F+, Hfr, Lfr & F’ strains with respect to the nature of the F-factor, the ability to convert recipients to donors, fate of transferred DNA, recombination frequency & probability of recombination for any given bacterial gene. xi. Describe the three conjugation mapping methods- interrupted mating, gradient of transfer and recombination mapping- used in constructing genetic maps of the bacterial chromosome. xii. Critically assess the three mapping techniques in relation to each other highlighting any advantages or disadvantages that might be associated. xiii. Perform problem-based solving in case studies involving conjugation mapping. Genetic Recombination in Bacteria – Transduction (mediated through a bacteriophage) i. ii. Define the term bacteriophage and distinguish between virulent and temperate phages. Compare and contrast the lytic and lysogenic infection cycles of λ-phage with the aid of appropriate diagrams. iii. Define and distinguish between the terms prophage & lysogen. iv. Describe in detail the production of generalized & specialized transducing particles. v. Compare and contrast generalized and specialized modes of transduction especially with respect to the type of phage life cycle employed, range of transducing capability, efficiency of transduction, probability of transduction for a given gene & fate of transferred DNA. vi. Distinguish between Hft-lysate and Lft-lysate. Genetic Recombination in Bacteria - Transformation i. ii. Define the term transformation as it relates to genetic recombination in bacteria. Explain in detail the mechanism of bacterial transformation, highlighting the steps and the essential requirements for the process to occur. iii. Describe Griffith’s experiment which demonstrated genetic recombination via transformation in Pneumococci bacteria. iv. Define the term competency and describe how competency can be artificially induced in bacteria. v. Discuss the factors that affect transformation efficiency. Transposition in prokaryotes (ability of genes to change position in the bacterial chromosome) i. Define the term transposition as the mobilization of genetic elements from one location in the genome to another. 82 ii. Describe and illustrate the structure of the three types of transposons: insertion sequence elements, composite and non-composite transposons and distinguish between them. iii. Explain the function of transposase enzyme in the mobilization of insertion sequence elements. iv. Describe the mechanism of transposition of insertion sequence elements and explain how target site sequences become duplicated. v. Discuss the role and significance of transposons in multiple drug resistance. Gene Fine Structure Analysis in prokaryotes i. ii. iii. iv. v. vi. Define complementation and distinguish it from recombination. Explain the difference the between recombination testing and complementation testing especially with respect to their uses. Describe in detail how recombination testing and complementation (cis-trans) testing are carried out. Discuss the merits and limitations of complementation tests. Construct complementation maps based on cis-trans test data. Define and distinguish between the terms cistron, muton & recon. Gene Fine Structure Analysis in viruses i. ii. Describe the inheritance of plaque morphology in bacteriophages. Describe the complementation spot test to determine whether mutations are in the same or different cistrons. iii. Describe the role of complementation and recombination in the mapping of the rII-locus in bacteriophage T4. iv. Describe Benzer’s deletion mapping technique and discuss its significance in gene fine structure analysis. Topic 3: Molecular genetics Molecular genetics- molecular organization of the eukaryotic genome i. ii. Outline the experiments that clearly showed that DNA is the genetic material. Discuss the components that make up the non-repetitive, moderately repetitive and highly repetitive sequences of the genome and the role of each of the three types of sequences. iii. Define the terms intron and exon and discuss the functions of each. iv. Critically discuss the evolutionary origin of introns: (introns first, introns early, introns late). v. Discuss the theories on the evolution of genes in light of the understanding of the variation of gene structure in organisms. Genomics i. ii. iii. iv. v. Define the term genomics and discuss the importance of genomic analysis. Describe and differentiate between functional and comparative genomics. Demonstrate knowledge of gene estimates for the human genome and discuss why previous estimates have been continually lowered. Discuss the organization and complexity of the human genome. Describe and discuss the functions and origins of non-genic sequences in the human genome: (STR’s, LINES, SINES, microsatellites, minisatellites & VNTR’s). 83 vi. Outline the evolution of concept of a gene and gene locus from the Mendelian concept to the modern concept using genomics. vii. Describe the role of STR’s and microsatellites in DNA fingerprinting. Genotypic function of DNA - DNA structure and models of replication i. Describe the structure of DNA including its double helical nature consisting of complementary antiparallel strands (opposite polarity) and its major components. ii. Define nucleotides as the building blocks of DNA molecules and state the constituents of a nucleotide monomer unit. iii. Differentiate between the terms nucleotide and nucleoside. iv. Describe in detail the Messelson & Stahl experiment which demonstrated the semi-conservative model of DNA replication. v. Describe and explain the experimental evidence to support a bi-directional mode of DNA replication. vi. Describe and distinguish between theta-mode (moving fork) and sigma-mode (rolling circle) replication. vii. Identify the enzymes involved in DNA replication as DNA polymerases and state the necessary requirements for successful DNA replication. viii. Explain what is meant by semi-discontinuous replication of DNA and describe the evidence that revealed this characteristic feature of DNA replication. ix. Define the terms leading strand, lagging strand & Okazaki fragment as they relate to semi-discontinuous DNA replication. x. Explain what is meant by the growing point paradox of DNA replication and how it has been resolved. xi. Define the term replisome as a multi-enzyme complex responsible for the replication of DNA. xii. Illustrate the structure of a typical prokaryotic replisome by means of a clearly labeled diagram and describe the functions of the constituent enzymes. xiii. Differentiate between the replication apparatus of prokaryotes and eukaryotes. xiv. Describe in detail the steps involved in DNA replication (initiation, elongation & termination) and how they are facilitated by the replisome. xv. Compare and contrast prokaryotic and eukaryotic DNA replication with particular emphasis on differences relating to the linear nature of eukaryotic chromosomes and the circularity nature of prokaryotic DNA molecules. Phenotypic Function of DNA - Transcription i. ii. iii. iv. v. vi. vii. Define the term transcription as it relates to the Central dogma of biology. Identify the enzyme involved in DNA transcription as RNA polymerase and describe its characteristic features including the essential requirements for its function. Explain in detail the steps involved in the process of transcription (initiation, elongation & termination). Compare and contrast the process of transcription in prokaryotes and eukaryotes. Draw and annotate the typical structure of prokaryotic and eukaryotic genes identifying the major differences between the two. Explain the consequences of the eukaryotic split-gene structure on the production of mature mRNA and the need to remove introns from heterogeneous mRNA by splicing. Describe and illustrate the steps involved in intron-splicing pathways that lead to the production of mature mRNA in eukaryotes. 84 viii. Describe the post-transcriptional modifications involved in the production of mature eukaryotic mRNA that affect mRNA stability: 3’-polyadenylation & 5’-capping. Regulation of Prokaryotic Gene Expression – lac Operon (negative control with superimposed positive control) i. ii. iii. Explain the importance of systems to regulate the expression of genes. Define the term operon and explain the advantage of gene expression using an operon system. Explain what is meant by a negative control system and a positive control system and be able to differentiate between the two. iv. Distinguish between the two types of negative control systems: inducible & repressible and give examples of such systems. v. Describe and illustrate the structure of the lac operon. vi. List and explain the functions of the structural genes of the lac operon along with the regulatory sequences involved in controlling expression of the genes. vii. Explain the differences between cis-acting and trans-acting control elements. viii. Explain in detail how the negative and positive control systems of the lac operon function to regulate expression of the structural genes. Regulation of Prokaryotic Gene Expression – trp operon (negative control with superimposed attenuation) i. ii. Describe and illustrate the structure of the trp operon. Identify and explain the functions of the structural genes of the trp operon along with the regulatory sequences involved in controlling expression of the genes. iii. Explain in detail how the negative control system of the trp operon functions to regulate expression of the structural genes. iv. Define the term attenuation and explain in detail how it is employed in the regulation of the trp operon. v. Explain the alternative TRAP mechanism of control of the trp operon in B. subtilis. Temporal control of genes in bacteriophages i. Describe and discuss how altering the specificity of RNA polymerase by modification of the sigma factor in the host by the SPO1 phage can result in transcription of the phage genes in a temporal manner. ii. Describe and discuss how anti-termination regulates the transcription of genes of the lambda phage in a temporal manner. Regulation of genes in eukaryotes: facultative and condensed chromatin, position effects, methylation i. Discuss how RNA polymerase cannot bind to chromatin in tightly coiled heterochromatic regions in eukaryotic genomes resulting in non-transcription of genes in that region. ii. Discuss how facultative heterochromatin is regulated to provide environmental flexibility. iii. Discuss how transcription of genes in the euchromatin can be affected by their proximity to the heterochromatic regions. iv. Discuss how methylation of genes prevents their transcription and this methylation can be heritable (epigenetics). Phenotypic Function of DNA - Translation i. Define the term translation as it relates to the Central dogma of biology. 85 ii. Describe the basic structure of prokaryotic and eukaryotic ribosomes and outline their role in the process of translation. iii. Describe the function of tRNA molecules in the process of translation. iv. Define the terms codon & anti-codon. v. Define the Shine-Dalgarno consensus sequence and explain its importance in the initiation of translation of prokaryotic mRNA. vi. Explain in detail the steps involved in the process of translation (initiation, elongation & termination). vii. Discuss how post translation modifications such as clipping, targeting proteins to organelles, protein folding, glycosylation and phosphorylation are important for functionality of the translated protein. The genetic code i. ii. iii. Describe the nature of the genetic code and explain what is meant by the following features: triplet code, non-overlapping code, commaless/gapless code, degenerate code & universal code. Describe the experiments that provided evidence to demonstrate the triplet nature of the genetic code. Explain how Crick’s Wobble hypothesis and iso-accepting species of tRNA enable the cell system to cope with the degeneracy of the genetic code. Assignments/ quizzes/ worksheet a. Multigene families By the end of this exercise students should be able to: i. ii. iii. iv. v. vi. Define multigene families Outline different types of multigene families Define multigene superfamily with examples Explain the origin of multigene families Describe how members of multigene families tend to retain more structural similarity than would be expected over evolutionary time Describe the evolutionary significance of multigene families. b. Epigenetics By the end of this exercise students should be able to: i. ii. iii. Explain why identical twins become epigenetically different as they age, based on their life histories Explain the role of epigenetic changes in the induction of cancers in humans Show how a human’s diet epigenetic imprint can be inherited in his/ her progenies. c. Bacterial transformation By the end of this exercise students should be able to: i. ii. iii. iv. Describe the process of introducing a GFP-plasmid into Xanthomonas axonopodis pv. dieffenbachiae using electroporation Relate the process to the normal process of bacterial transformation. Explain the role of the GFP fluorescence as a selectable marker Outline the advantages of GFP fluorescence over selection on specific growth media supplemented with antibiotics 86 v. State the factors which influence transformation efficiency. d. Worksheet on conjugation mapping in bacteria By the end of this worksheet students should be able to: i. ii. Calculate genetic distances between bacterial genes Construct genetic maps of bacterial chromosomes based on case studies using the methods of interrupted mating, gradient of transfer and recombination mapping. Course assessment In-course Incourse examinations will include multiple choice questions, structural and short answer type questions. These exams will assess knowledge, comprehension, application and analysis of course content. The quizzes for the assignments will allow students the opportunity to read, comprehend, interpret, analyze and summarize reports written in the scientific manner and as well reinforce and extend what is learned during class time. The worksheet on conjugation will allow students to calculate genetic distances and construct genetic maps based on the three methods (interrupted mating, gradient of transfer and recombination mapping). (See the course calendar for the assessment schedule). Assessment In-course Examination #1 In-course Examination #2 In-course Examination #3 Class quiz #1 Class quiz #2 Class quiz #3 Worksheet Final Examination Total Weighting 10% 10% 10% 5% 5% 5% 5% 50% 100% The final examination comprises multiple choice questions, short answer questions with four to five parts, structured questions and essay type questions. These questions are meant to test students’ ability to recall, comprehend, apply and analyze course content in a critical and logical manner. Students are required to answer ALL questions. Overall assessment Incourse 50% Final Theory Exam (2 hours) 50% 87 Evaluation Two student representatives will be elected from the class. Student feedback will be obtained officially from the staff-student liaison meetings held twice per semester. However, students are encouraged to give their feedback on the course via myelearning at any time. Comments will be assessed critically by the instructor, course coordinator and first examiner and weaknesses will be addressed to improve on the course in the future. Teaching Strategies The course consists of 33 lectures, THREE assignments (for quizzes), ONE worksheet and THREE tutorials. The course is myelearning supported and your manual contains specific course objectives as well as lecture outlines, past exam papers and model answers. Recommended Texts 3. Brooker, Robert J. Genetics: Analysis & Principles 3rd Edition McGraw Hill Higher Education 2008. ISBN: 9780071287647 4. Snustad, D. Peter & Simmons, Michael J. Principles of Genetics 5th Edition John Wiley & Sons Inc. 2009. ISBN: 9780470388259 5. Genetics: A Conceptual Approach (Benjamin A. Pierce) 6. Principles of Genetics 7th Edition (Robert H. Tamarin) 7. Genes VIII (Benjamin Lewin) 8. Molecular Cell Biology (Lodish et al.) 9. Molecular Biology of The Cell (Alberts et al.) Course Calendar Course Delivery: Topics covered Week Lecture Subjects 1 Course Overview Introduction to Cytogenetics: Chromosome primary & secondary structure chromosome ultra-structure- Chromatin/ Distribute paper on Evolution of Cytogenetics Multigene Families ** Chromosomal Macromutations: Deletions 2 Chromosomal Macromutations: Duplications-1 Chromosomal Macromutations: Duplications-2/ Quiz #1- Evolution of multigene families** Chromosomal Macromutations: Inversions 3 Chromosomal Macromutations: Translocations-1 Chromosomal Macromutations: Translocations-2 Ploidy: Autopolyploidy 4 Ploidy: Allopolyploidy; Aneuploidy Epigenetics & Chromatin Dynamics-1/ Distribute paper on Epigenetics** 88 Epigenetics & Chromatin Dynamics-2 5 Homeobox genes In-course Examination #1 Genetic Transfer in Bacteria: Conjugation 6 Prokaryotic genetics Conjugation Mapping Genetic transfer in bacteria: transduction and transformation/ Quiz #2: Epigenetics** Prokaryotic genetics: transposition 7 Gene Fine Structure Analysis in Bacteria: Recombination Testing & Complementation Testing/ Distribute worksheet on conjugation mapping** Gene Fine Structure Analysis - bacteriophages: Deletion Mapping In-course Examination # 2 8 Molecular genetics- organization of the eukaryotic genome Molecular genetics Molecular genetics- organization of the eukaryotic genome continues/ Distribute paper on bacterial transformation** Genomics/ Collect worksheet on conjugation mapping** 9 DNA Structure & Models of Replication DNA Structure & models of replication continues DNA Structure & models of replication continues 10 Phenotypic function of DNA: Transcription (Prokaryotes) Phenotypic function of DNA: Post-transcriptional Processing of Eukaryotic mRNA/ Quiz #3: Bacterial transformation** Regulation of Prokaryotic Gene Expression: negative control- lac Operon 11 Regulation of Prokaryotic Gene Expression: negative control with superimposed attenuation- trp Operon; positive control Temporal control of genes in bacteriophages Control in eukaryotes- facultative and condensed heterochromatin, position effects, methylation 12 Translation and post translational regulation In-course Examination #3 Tutorial 13 Tutorial Tutorial Additional information 89 Attendance Attendance in the incourse exams and the quizzes is mandatory. Any student who misses an incourse exam or a quiz is advised to consult immediately in person or by email with the course instructor regarding their make-up options. Absence must be accompanied by a written excuse or medical submitted to the Main office, Life Sciences within 7 days of the missed session. Any student who was inexcusably absent or who does not write an incourse exam, a quiz or the worksheet will receive 0% for that exercise. How to study for this course You are encouraged to work together in small cohesive groups as much as possible to go through the course content. As we go through the various topics, students should attempt to answer all the sample questions placed on myelearning and discuss the answers amongst themselves. All comments, questions and concerns provided on a particular topic will be addressed during class time, via myelearning or FaceBook (account to be created). Your departmental course textbook on myelearning contains all the topics to be taught and the textbook content is aligned similarly as the lectures; please read the textbook. Use the responses and comments for your incourse examinations, quizzes and worksheet as a guide to answering the questions properly. There are several pass paper questions in the library and you are encouraged to attempt these questions. Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 90 THE UNIVERSITY OF THE WEST INDIES ST. AUGUSTINE FACULTY OF SCIENCE AND TECHNOLOGY DEPARTMENT OF LIFE SCIENCES Course code: BIOL 2265 Course Title: Fundamentals of Microbiology Number of Credits: 3 Level: Undergraduate –Year II Semester: 2 Pre-requisites: Either BIOL1262 Living Organisms I and BIOL1263 Living Organisms II or BIOL1261 Diversity of Organisms or (BIOL1065 Diversity of Plants and Animals and AGRI1012 Microbiology) and either BIOL1362 Biochemistry I and BIOL1364 Genetics I or BIOL1061 Cell Biology and Genetics. Anti-requisites: BIOL2263 General Microbiology Course description An overview of the biology, taxonomy and phylogeny of the bacteria, fungi and viruses. Bacterial genetic recombination, growth, nutrition as well as carbon and energy metabolism. Principles of classical and molecularbased methods used in identification and enumeration of microorganisms. Significance/rationale Microbiology is one of the core sub-disciplines of biology. Microorganisms have been on earth for billions of years and have influenced the physical and chemical conditions necessary for the evolution of higher forms of life. They represent by an unimaginably long distance the largest biological diversity on earth and their activity is essential to the survival of humans on earth. Although some microbes are responsible for human, animal and plant diseases they contribute positively to human welfare as they play critical roles in food & agriculture, industry, medicine and the environment. This course covers an overview of the biology, taxonomy and phylogeny of the archaebacteria, eubacteria, fungi and viruses. Bacterial genetic recombination, growth, nutrition as well as carbon and energy metabolism will be covered. Additional topics include traditional and molecular based methods used in analytical and diagnostic microbiology. Purpose of Course: The course provides fundamental training in theoretical and practical microbiology as part of the major in biology in the Department of Life Sciences, UWI, St. Augustine. The course will also be useful to students who may have an interest in other disciplines which have microbiological applications such as environmental biology and ecology, remediation technology, microbial natural product chemistry, biotechnology, biochemistry, medicine. This course will also serve as the prerequisite for the more specialized Level III courses in microbiology and related disciplines including microbial biotechnology and the courses in the minor programme in microbiology. Instructor Information BIOLXXXX will be managed by a course coordinator and would be taught be a team of experienced lecturers, supported by a Teaching Assistant/Instructor and laboratory demonstrators. Office hours for the coordinator 91 and lecturers are posted on the faculty bulletin boards and are available from the Department of Life Sciences’ general office. Team members can also be contacted via e-mail. Name: Adesh Ramsubhag (Co-ordinator) E-mail: [email protected] Office location: Rm. 220, Natural Sciences Building, Old Wing Office hours: Check Notice Boards Phone: 662-2002 Ext. 83086 Name: XXXX (Teaching assistant) E-mail: Office location: Office hours: Phone: 662-2002 Ext. Letter to students Welcome to BIOL-XXXX- Fundamentals of Microbiology. This course covers the fundamental biology of major groups of microorganisms including bacteria, fungi and viruses. Significant attention is also placed on covering the basic methods used in microbiology. The course is taught through a series of 18 lectures, 12 tutorials and 12 hours of labs. The course is quite extensive and students must make a sincere effort to do well. It is important to attend lectures in order to benefit from more in depth analysis and details that cannot be gained from downloading and reading the PowerPoint and video presentations of the lectures. Please make use of supplementary information provided to strengthen foundation and enhance understanding of material covered. Content: BIOLXXXX covers the following content: Microbial diversity Microbial growth and nutrition Bacterial genetic recombination Fungi Viruses Carbon and energy metabolism of chemotrophs Bacterial photosynthesis Control of microorganisms Enumeration of microorganisms Biochemical and molecular identification of microorganisms Immunology and serology Microbial ecology Course goal: The goal of the course is to provide student with general knowledge on the biology of microorganisms and develop basic practical skills used in their study. General Objectives: Students completing the course will have: 1. A fundamental understanding of the diversity, taxonomy, physiology and genetics of bacteria, fungi and viruses; 92 2. Basic practical skills used in microbiology 3. An understanding of the means to controlling microorganisms Learning objectives: Upon completing this course, students will be able to - describe the general features of microbial diversity and structure and function of prokaryotic and eukaryotic cells; - review of diversity and importance of microorganism to humans and environment; - differentiate and identify major taxonomic groups of fungi and fungal-like organisms based on morphology, nutrition, reproduction and ecology; - describe the general structural, genetic and reproductive characteristics of viruses - describe the general characteristics and life cycles of named bacteriophages and animal viruses; - describe the general characteristics of the HIV virus ; - explain how the virus affects humans in causing AIDS as well as how the disease is managed; - explain the different ways by which genetic changes can take place in bacteria; - explain the function of different types of culture media; - explain carbon and energy metabolic processes of microbes; - compare and contrast carbon and energy metabolism between chemoheterotrophs and chemoautotrophs; - compare and contrast chemotrophic and phototrophic respiration; - explain how intrinsic and extrinsic factors affect microbial growth and activity; - describe the mechanisms used by microorganisms to survive harsh/extreme conditions; - describe the typical bacterial growth curve and explain the processes influencing and/or taking place at each phase; - explain how growth process can be controlled under continuous culture conditions; - explain how microbial cultures can be isolates and purified; - describe the different way to control microorganisms; - explain the effect of different control factors on microbial cells; - explain how the effectiveness of disinfectants can be assessed; - explain the principles of classifying and naming microorganisms using conventional and current taxonomic tools; - explain the differences between Eubacteria and Archaebacteria; - compare and contrast the different methods use in enumerating microorganisms; - describe and explain the interactions of among microorganisms and between microorganisms and other forms of life; Mode of Delivery: Lectures -18 hours: Didactic; interactive Tutorials -12 hours: Interactive; mind maps; problem-solving Laboratory classes- 12 hours (4 three- hour sessions): Interactive practical tasks; problem-solving ASSIGNMENTS: Coursework for BIOLXXX will be assigned as follows: 9. Writing across curriculum exercises-5%. 93 These activities are designed to encourage students to become active learners by engaging knowledge to further develop understanding. The exercises would help students understanding content and expressing ideas. A total of five simple exercises worth a total of 5 marks (1 mark per exercise) would be given during lectures or tutorials (no more than one per week). Each exercise would take between 5 – 10 minutes to complete and would comprise of varying activities including: a. Focused free-writing- Students may be asked to briefly write on their understanding or perspective on a subject/topic covered or on how the subject/topic may be connected to other situations. b. Entry slips- Short responses to questions posed at the beginning of class; c. Answering questions. One or few questions would be given which students must answer and submit for grading. d. Short summary. Students are asked to summarize main points of a reading assignment in a short paragraph. e. Group presentation- The tutorial class would be divided into groups and each group would be required to make a short presentation on a document provided. f. Complete the life cycle: Students are asked to complete the life cycle diagram given to demonstrate their knowledge of the sequence of events and structures which occur in the microbial organism’s life cycle. g. Construction of dichotomous key: Students are asked to construct a dichotomous key to which can be used to key out (identify) a group of specimens. h. Reflective statement: students should write a reflective statement on one of the labs completed: students should identify problems encountered, techniques mastered or improved, what was done well and why and what was learnt and what is unclear. 10. In-course Test-20% One written in-course test would be given worth 10% per test in week 7. Two online tests will also be given in weeks 4 and 11 work 5% each. The tests would comprise of MCQs and structured questions. 11. Laboratory reports-20% Students are required to submit two full lab reports worth 4% each in addition to four completed worksheets worth 2 mark each. Two lab quizzes would also be given during the 2nd and 4th practical sessions. Each lab quiz would worth 2 marks each. 12. Class participation- 5% Student participation in tutorial sessions will be monitored by the tutor and a grade assigned at the end of the semester. Participation shall include more than just showing up at tutorial – some evidence of an active role in the tutorial discussion would be required to obtain the full 5% for participation. At the beginning of the semester, the students in each tutorial would engage in developing the exact assessment criteria for this assignment, and the student-developed criteria will be used to determine each student’s participation grade. Cheating, including plagiarism, would not be tolerated. Incidents of cheating would be dealt with according to UWI’s rules and regulations including: - Examination Regulations for First Degrees, Associate Degrees, Diplomas and Certificates. Section (B) Cheating. Regulations 96–102; http://sta.uwi.edu/resources/documents/Exam_and_GPA_regulations.pdf. - University Regulations on Plagiarism (First Degrees, http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf. Diplomas and Certificates). 94 Assessment: Course Work: 50% - Writing across the curriculum; critical thinking exercises - Incourse test o One written tests o Two online tests - (2 X 5%) - Practical exercises o Laboratory reports (2 full reports X 4% and 4 worksheets X 2% ) o Laboratory quizzes (2 X 2%) - Tutorial Attendance and participation Final Semester Examination: - One 2-hour comprehensive written paper comprising of o 35 MCQs worth 35% of paper o 5 short answer questions worth 35% of paper o 1 essay-type question worth 30% of paper o All questions in this paper shall be compulsory 5% 10% 10% 16% 4% 5% 50% EVALUATION: BIOLXXXX will be evaluated in two ways – (a) through the offices of the Class Representative and the Life Sciences Student-Staff Liaison Committee, and (b) an end of semester course evaluation survey. The class will elect four class representatives (one per lab stream), whose role is to act as a mediator between the Life Sciences academic staff and the students in the class. The representatives will attend Liaison Committee meetings (held at least twice per semester), where they will present feedback on the course to the Department for action. The UWI performs a course evaluation survey at the end of every semester, and this information will also be used for overall assessment of the course and guide possible actions for improvement in subsequent semesters. TEACHING STRATEGIES: A combination of teaching strategies will be adopted in BIOLXXXX. The primary teaching strategy will be based on the face to face classroom lectures and discussions. Videos of lecture presentations would be made available to students prior to the lecture session via podcast media. Students are advised to view these videos before the lecture so that more emphasis can be placed on discussions and answering questions, thereby facilitating deep learning. This would also afford the time to have assessment exercises on “writing across the curriculum”. MyeLearning will be utilized throughout the course as a means to provide access to course materials such as Powerpoint presentation files, animations, weblinks, lessons and quizzes. This medium would also be used as a portal for student-lecturer communications and the dissemination of coursework feedback. A total of 12 tutorial sessions would be given for the semester. These would be small group sessions which would be conducted by instructors or tutors, who would normally by senior postgraduate students in Life Sciences. Students must attend tutorial sessions. Tutorials sheets will be assigned prior to each session via myeLearning, and students are expected to attempt the solutions before coming to tutorial. The goal of the tutorial session is to give students a more hands-on experience with the course material and easier access to course instructors. Students will be expected to ask and answer questions on material that is unclear, propose solutions to questions on the tutorial sheet and to generally participate fully in the tutorial activities. Tutors will not merely be going through the answers to the tutorial questions in these sessions. 95 Practical sessions would include demonstrations and problem solving exercises to develop practical skills and enhance understanding of content covered in the theory section. These exercises would include analysis of specimens, experiments and use of identification keys to assign organisms into different levels of taxa. In order to pass the course, you must gain an overall passing mark of 40%. Any student who misses more than 25% of practical classes or tutorial sessions without a medical or other valid excuse can be debarred from writing the final exam. RESOURCES: Text books: - Willey, J.M., Sherwood, LM., and Woolverton, C.J. (2010) Prescott’s Microbiology (8th Edition), McGraw-Hill, New York - Benson, H.J (2002), Microbiological Applications (Eight Edition), McGraw-Hill, New York Suggested additional readings: - Madigan, M and Martinko, J (2005), Brock Biology of Microbiology (Eleventh Edition), Prentice Hall, New Jersey Teaching materials: - Electronic copies of presentations as well as supplementary handouts will be made available to students; - Internet links to web-based resources will be provided to students Lab coat: - Each student should have suitable Lab coat to use for practical exercises. Students not having lab coats will not be permitted in the lab. COURSE CALENDAR/SCHEDULE: Introduction and review Microbial growth and nutrition Fungi Viruses Carbon and energy metabolism of chemotrophs Bacterial photosynthesis Control of microorganisms Bacterial genetic recombination Enumeration of microorganisms Biochemical and molecular identification of microorganisms Immunology and serology Microbial ecology 96 Course calendar: Week 1 Practical exercise Lecture/Tutorial Lecture 1: Introduction; microbial diversity and importance to humans and the environment Lecture 2: Microbial growth and nutrition Tutorial 1: 2 Lecture 3: Fungi and fungal-like organisms Tutorial 2: 3 Review of basic aseptic and microbiological methods Isolation of bacteria and partial establishment of Koch’s Postulates Lecture 4: Fungi and fungal-like organisms Lecture 5: Viruses Tutorial 3: Staining and microscopic examination of bacteria Morphological diversity of bacteria 4 Lecture 6: Viruses Tutorial 4: Online test- MCQs (5%) 5 Lab quiz based on previous session Lecture 7: Bacterial genetic recombination Isolation and Enumeration of fungi Lecture 8: Bacterial genetic recombination Observation and morphological identification of Fungi Tutorial 5: Observation of viral infected plants 6 Lecture 9: Carbon and energy metabolism Tutorial 6: 7 Lab quiz based on previous sessions Lecture 10: Carbon and energy metabolism Enumeration of Identification of bacteria based on biochemical tests Tutorial 7: 8 Written incourse test (in lecture slot) – MCQs, structured questions (10%) Lecture 11: Bacterial photosynthesis Tutorial 8: 9 Molecular identification of bacteria, fungi and viruses Lecture 12: Control of microorganisms Lecture 13: Biochemical microorganisms and molecular identification of Tutorial 9: 97 10 Lecture 14: Biochemical microorganisms and molecular identification of Lecture 15: Immunology and serology 2 Tutorial 10: 11 Lecture 16: Immunology and serology 2 Lecture 17: Microbial ecology Tutorial 11: Online test- MCQs (5%) 12 Lecture 18: Microbiology ecology Tutorial 12: Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 98 THE UNIVERSITY OF THE WEST INDIES ST. AUGUSTNE FACULTY OF SCIENCE AND TECHNOLOGY DEPARTMENT OF LIFE SCIENCES Course code: BIOL 2360 Course Title: Biochemistry IIA Credits: 3 Level: 2 Semester: 1 Pre-requisites: Either BIOL1362 Biochemistry I or BIOL 1061 Cell Biology and Genetics and either CHEM 1062 Basic Chemistry for Life Sciences or CAPE Chemistry or CHEM0060/1 Preliminary Chemistry I & II and either BIOL1262 Living Organisms I or BIOL1263 Living Organisms II or BIOL1261 Diversity of Organisms. Anti-requisites: BIOL2361 Biomolecules & Energy Metabolism; BIOL2365 Comparative Biochemistry Course Description Biochemistry is a fundamental sub-discipline of Biology. This course starts off by exploring the question – Why is life thermodynamically possible? We will also examine how we digest and metabolize sugars, fats and proteins. Subsequently, we will discuss the roles of the main hormones involved in the fed and fasting states and finally we will look at two common metabolic disorders in the Caribbean, diabetes and obesity. Course Rationale: BIOL 2XXX is a core course for the Biology Major in the Department of Life Sciences. This course builds on the topics covered in the year 1 Biochemistry course, BIOL 1362. It covers key biochemical concepts required for understanding modern Biology. Materials covered in this course will be an asset for students who wish to further their studies in fields of medicine or nutrition or wish to pursue careers in teaching or the pharmaceutical industry. Instructor’s information: Mr. Jason Matthew Email: [email protected] Facebook: BiochemJM Blog: http://biochemjm.wordpress.com/ YouTube: Biochem JM; http://www.youtube.com/user/BiochemJM/featured Website: https://sites.google.com/site/biochemjm/ Phone: 662-2002 ext 82080 Office: Old Nat Sci Wing, 2nd Floor; Rm 318 Letter to the students: Dear Biochemians, Congratulations on passing your first year courses. We look forward to guiding you on the rest of your journey here at the UWI. We shall implement a learner-centric approach to this course. There are screencast versions of all the BIOL 2365 lectures so that you can follow and learn at your own pace. This, however, is not a one-way service. You too will be required to create 15-minute YouTube videos of topics from the syllabus. You will have 99 access to a database of student videos from previous years so that you can get a sense of what is required from your videos. I am hoping that you get a better understanding of the topics by seeing it explained by your peers. You will be networking and collaborating with students from the other UWI campuses as well as globally via my BiochemJM Blog and Facebook services. Together, we shall challenge the traditional roles in the classroom and have students present and teach the course. The classroom will be filled with interaction and discussion. It is my sincerest wish that you learn from this course by challenging yourself to think outside the box. Remember, the riskiest thing you can do in life is not to take chances. The safest thing you can do is be remarkable. Imagine, create and innovate. See you in class, Biochem JM Content: Topics to be covered in this course include: Bioenergetics Membranes Enzyme action Carbohydrate metabolism Lipid metabolism Nitrogen metabolism Integration of metabolism Goals / Aims: At the end of this course, students will be able to: Describe the major metabolic pathways involving carbohydrates, lipids and nitrogen containing compounds, highlighting certain key enzymatic reactions for their regulatory importance Apply what they have learnt in the metabolism section of the course to some everyday metabolic disorders such as diabetes and obesity Explain the mechanism of enzyme action and how their activities in the cell are controlled Explain the thermodynamics of high- energy compounds and redox chemistry. Apply critical thinking and creativity to explain selected biochemical concepts Demonstrate effective communication skills in the areas of interviewing and presenting Demonstrate project management skills and actively use podcasts as a medium for positive student expression. Brainstorm and design themes and content for your podcast Complete pre-production and production of your podcast and create a high-quality video podcast Learning Outcomes: Topic 1: Orientation (1h) At the end of this section, students should be able to: Appreciate the rationale for this course State what is expected from them in this course – attendance of tutorials, labs etc State how they will be assessed throughout this course Navigate and access the Facebook, Blog, YouTube , Website and myelearning services for this course Basic manipulation of video editing software – MS MovieMaker, iMovie, and FinalCut Pro Create a podcast using CamStudio Start preparing their group video project (This will be supplemented with instructional YouTube videos specifically created for this orientation session) Topic 2: Bioenergetics (1h) Gibbs free energy, enthalpy, entropy, equilibrium constant, mass action ratio, ATP, thioesters 100 At the end of this section, students should be able to: Draw energy profile diagrams Explain what makes a process spontaneous Distinguish between ΔG and ΔGo’ Explain how the change in free energy depends on reactant concentrations Discuss how a thermodynamically unfavorable reaction can occur invivo Explain the structural basis for the high group transfer potential of ATP Explain how phosphorylated compounds, thioesters and reduced cofactors transfer free energy Explain why cells control metabolic reactions with large free energy changes Active learning topics: The role of phosphocreatine in muscle, comparison of ATP generation in sprinters vs. distance runners or migratory birds vs. birds that rarely fly Topic 3: Membranes (3h) Fluid mosaic model, the thermodynamics of membrane transport, membrane potential, action potential, porins, gated channels, membrane fusion, acetylcholine, SNAREs At the end of this section, students should be able to: Describe the fluid mosaic model of membrane structure State the factors which limit membrane protein mobility Explain why glycoproteins and glycolipids face the cell exterior State what is a membrane potential Calculate the membrane potential when ion concentrations are known Describe the role of the cell membrane in maintaining membrane potential Describe how an action potential is generated and propagated Determine when transmembrane movement of a substance is thermodynamically favorable Compare active and passive transport Compare Na,K-ATPase to other transport systems in terms of mechanism and energy requirement Summarize the role of ATP in P-type ATPases and ABC transporters Compare secondary active transporters to other transporters in terms of mechanism and energy requirements Describe the sequence of events at the nerve muscle synapse Describe how the structure of the SNARE complex is related to its function Explain why changes in bilayer curvature are required for exocytosis Active learning topics: Olestra, spicy Indian dishes and whole-milk yogurt, aquaporins, succinylcholine (muscle relaxant), lidocaine (anesthetic), digitalis, botulinum toxin Topic 4: Enzyme Action (1h) Substrate channeling. Control of enzyme activity. At the end of this section, students should be able to: Using the PDH complex describe the mechanism and benefits of substrate channeling regulation of enzyme activity Topic 5: Carbohydrate metabolism (4h) Glycolysis (recap), gluconeogenesis, glycogen metabolism, pentose phosphate pathway, Cori cycle 101 At the end of this section, students should be able to: Recall from BIOL 1362 course (pre-req) the role of glycolysis, its enzyme reactions and how it is regulated Describe the reactions of gluconeogenesis Describe how glucogenic precursors can give rise to glucose Compare and contrast the glycolysis/gluconeogenesis pathways Describe the Cori cycle Describe glycogen metabolism (synthesis and degradation pathways). Illustrate both the oxidative and non-oxidative branches of the pentose phosphate pathway and explain the importance of this pathway to cells with respect to providing unusual monosaccharides, biosynthetic reducing equivalents and intermediates for both synthesis of nitrogenous bases and glycolytic intermediates Active learning topics: Glycogen storage diseases, hemolytic anemia, alcoholism and hypoglycaemia, Topic 6: Lipid metabolism (5h) Digestion, mobilization and transport of fats. De novo biosynthesis of fatty acids – fatty acid synthase enzyme complex. Role of acetyl CoA carboxylase. Oxidation of fatty acids. Ketogenesis. At the end of this section, students should be able to: Describe the role of bile and pancreatic lipase in digestion of dietary lipids Describe the role of lipoprotein lipase Describe the fate of glycerol in the body State the different plasma lipoproteins and describe how they are different Explain the roles of lipoproteins and apolipoproteins Describe the committed step of fatty acid synthesis by showing that acetyl CoA carboxylase is a bifunctional enzyme Describe the reactions of the De Novo synthesis of fatty acids Discuss how fatty acids can be synthesized in the liver from carbohydrates Describe the role of hormone sensitive lipase Describe the carnitine shuttle mechanism Describe the reactions in fatty acid oxidation Compare the oxidation of saturated, unsaturated and odd-chain fatty acids Compare fatty acid synthesis with oxidation State the conditions for ketogenesis Give examples of ketone bodies Describe the reactions involved in ketogenesis Active learning topics: MCAD deficiency and oxidation, -oxidation is a chemical source of water for desert animals, brown adipose tissue, white adipose tissue lacking glycerol kinase, LDL and cardiovascular disease, Orlistat, ketonuria Topic 7: Nitrogen Metabolism (3h) Nitrogen fixation, protein degradation (endogenous, dietary), transamination, oxidative deamination, urea cycle, 102 nucleotide metabolism. At the end of this section, students should be able to: Describe the structure of the nitrogenase complex and state how its structure is suited for the reaction it catalyzes State the reaction of glutamine synthetase. State what is an essential amino acid Describe the mechanisms for degrading endogenous proteins State the enzymes and organs involved in digesting dietary proteins Describe transamination reactions using ALT and AST enzymes State the reactions of glutamate dehydrogenase Explain how transamination, oxidative deamination and reductive amination are involved in amino acid metabolism Describe how ammonia is transported to the liver Describe the reactions of the urea cycle State signs and symptoms of urea cycle enzyme deficiency diseases. Outline the roles of nucleotides in the cell State which amino acids are required for purine and pyrimidine de novo synthesis State the regulated step for purine and pyrimidine de novo synthesis Compare CPSI to CPSII Active learning topics: Urea cycle enzyme diseases, gout, phosphocreatine, NOS Topic 8: Integration of Metabolism (5h) Well fed and fasting states, roles of insulin and glucagon. At the end of this section, students should be able to: Classify insulin and glucagon as peptide hormones Describe the mechanism of action for insulin and glucagon Describe the metabolic effects of insulin and glucagon including the organs where these effects are taking place. Explain how glycolysis and gluconeogenesis are reciprocally regulated Explain how glycogenesis and glycogenolysis are reciprocally regulated Explain how fatty acid synthesis and fatty acid breakdown are regulated Discuss how malonyl CoA affects lipid and carbohydrate metabolism Active learning topics: Diabetes, obesity, metabolic syndrome. Course Assessment Overview Coursework Final exam 50% 50% Coursework assessment (50%): Biochemians Got Talent (BGT; 15%): Students are asked to create 15-minute videos on any topic off the BIOL 2365 course syllabus. (More details about this project are given in Appendix I; pg 12) 103 Case Studies (10%): a problem-based approach involving ‘real life’ situations will be used for selected topics of the syllabus to encourage problem-solving and critical thinking skills. Bring Your Notes End of Semester exam (10%): A 1 hour written exam covering all topics from weeks 1 - 11. Students will be given a note card from the instructor in week 8 of the semester. Students will be allowed to write whatever notes they want on the note card, which they are allowed to bring into the exam in week 12. Class participation and continuous assessment (5%): Students will be assessed continuously throughout the semester using a variety of strategies: 1. answering 10 minute quizzes on Moodle or in the classroom. These quizzes will be based on the basic concepts of the course. 2. contributing to the BiochemJM blog Practical (10%): Carbohydrate metabolism At the end of this practical, students should be able to: Understand specific enzyme catalyzed reactions in carbohydrate metabolism Interpret kinetic data for enzymes – rates of reaction and inhibition Use various techniques including micropipetting and spectrophotography Write up a biochemical lab report Final exam (50%): 2-hour written examination at the end of the semester consisting of three sections: Section A 25 MCQ (25%) Section B True and False, matching, fill in the blanks (25%) Section C Essay type questions (50%) ALL questions on paper are compulsory. Evaluation: Feedback on the course will be obtained informally from students on an ongoing basis by regular interactions and meetings among students, demonstrators and teaching assistants. Formal feedback will be via election of Class Representatives who sit on the Departmental Student-Staff Liaison Committee meetings held twice during the semester. Class reps will channel both concerns and commendations to the meeting as guided by the Department’s Standard Operating Procedures. Formal evaluation of the entire course will be accomplished via a UWI Course Evaluation questionnaire administered anonymously and confidentially at the end of the semester. All feedback will be considered on an ongoing basis and corrective action or adjustments made or discussed with students promptly or incorporated the following year. Teaching Strategies: Face to face time in the classroom Highly interactive Students will be making presentations (mostly on the active learning topics outlined for each topic) in class Question and answer game show type of sessions This course is Moodle supported. Links to podcasts and useful websites will be posted Links to open source PDF journal articles will be posted 104 Students will have access to course outline, lecture schedule and assignments (instructions and questions) Means by which students will be quizzed online Students will access their coursework marks Official form of communication between instructor and students The BiochemJM YouTube channel Screencast versions of all the BIOL 2365 lectures have been posted on this channel. Students can learn at their own pace and it frees up the actual face to face time for more interactive activities in the classroom Student created videos. Students create 15 minute videos on any topic of the syllabus and post them up on the BiochemJM channel. This will enable peer review learning with students in the course as well as students regionally and globally The Biochem JM Facebook page and the BiochemJM Blog Students have an avenue to reflect about the course with their peers Exchange of ideas between fellow students as well as international students Mentorship from past students who did the course Real time communication with the instructor Discussion forum Resources: Text Books (Library resources) and Lab. Requisitions (Department’s resources) are available. Department of Life Sciences & Main Library Computer labs to access online services. Readings: Main Text C.W. Pratt and K. Cornely: Essential Biochemistry 2nd Edition. Wiley & Sons 2011 Secondary texts J.M. Berg, J.L. Tymoczko and L. Stryer: Biochemistry 7th Edition. W.H. Freeman and Co. P.C. Champe, R. A. Harvey and D. R. Ferrier: Lippincott’s Illustrated Reviews 3rd edition. Lippincott Williams & Wilkins D. L. Nelson and M. M. Cox: Principles of Biochemistry 5th Edition. W.H. Freeman and Co. Course Calender (subject to review) Week Lecture subjects 1 Orientation [1] Bioenergetics [1] 2 Membranes [2] 3 Membranes [1] Enzyme action [1] 4 Carb Metab [2] 5 Carb Metab [2] Tutorials / Assignments / Labs Tutorial#1 Tutorial #2 105 6 Lipid Metab [2] Lab: Carbohydrate metabolism 7 Lipid Metab [2] Case Study #1; Tutorial #3 8 Lipid Metab [1] Nitrogen Metab [1] Tutorial #4 9 Nitrogen Metab [2] Case Study #2 10 Integration of Metab [2] Tutorial #5 11 Integration of Metab [2] Tutorial #6 Video Project is due 12 Integration of Metab [1] End of Semester Exam [1] 13 Review [ ] = number of hours Additional information: Refer to the Life Sciences Undergraduate Handbook for general guidelines for teaching, assessment, student conduct, essential skills, and support at http://sta.uwi.edu/fst/lifesciences/documents/handbook.pdf . Refer to the General Information and General Regulations in the Faculty Booklet available from http://sta.uwi.edu/resources/documents/facultybooklets/ScienceTechUndergrad.pdf . Students are reminded that they must attend a minimum of 75% of the practical sessions and tutorials. Failure to do so will result in debarment from the final examination. As a general principle, medicals or other excuses may only excuse a student’s presence at an assigned time. Students must still complete the assigned work (make-up lab report or make-up test) in order to obtain the marks for that item of coursework. The student is responsible for liaising with the Course Coordinator or Teaching Assistants to ensure the assigned make-up is completed. Students are hereby informed that plagiarism is forbidden and all unsupervised coursework items must be accompanied by a Coursework Accountability Statement in order to be assessed. Specific items may require submission through Turnitin on myeLearning. Refer to ‘University Regulations on Plagiarism’ available from http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf HOW TO STUDY FOR THIS COURSE: Attendance is mandatory for lectures, tutorials and practicals. Prior preparation is strongly advised to able to fully participate in activities and obtain the full value of the sessions. Thorough use should be made of the resources provided and students are strongly advised to become familiar with them and start utilising them from the first week. Regular updates on course progress and materials are also highly recommended and you should be checking into myelearning on a frequent regular basis to review materials, assignments and activities. Students are encouraged to interact regularly with staff on their projects, even outside of the assigned tutorial times to ensure prompt, satisfactory solution of any problems and to monitor progress. 106 Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 Appendix I: BGT instruction manual The University of the West Indies The Faculty of Science and Technology Life Sciences Department BIOL 2365: Comparative Biochemistry Biochemians Got Talent (10%) Imagine, Create, Innovate Lecturer in charge of project: 107 Mr. Jason Matthew Email: [email protected] Facebook: BiochemJM Blog: http://biochemjm.wordpress.com/ YouTube: Biochem JM; http://www.youtube.com/user/BiochemJM/featured Phone: 662-2002 ext 82080 Desired outcomes: After completing this project you should be able to: Apply critical thinking and creativity to explain selected biochemical concepts Demonstrate effective communication skills in the areas of interviewing and presenting Demonstrate project management skills and actively use podcasts as a medium for positive student expression. Brainstorm and design themes and content for your podcast Complete pre-production and production of your podcast and create a high-quality video podcast. Navigation: Topic Page Your mission 3 Groups 3 Available resources 4 Suggested programs to use 4 Acceptable video file format 4 Important dates 5 Final submission 6 Late submission 6 Project outline rubric 7 Final submission rubric 8 Assessment overview 9 Copyright Guidelines 9 108 Your Mission: Students are required to make a 15-minute video covering topic(s) or concept(s) taught in this BIOL 2365 course. The methodologies used in this project should only be limited by your imagination, creativity, innovation and sadly by financial resources. Here are some possibilities: Songs Plays/skits Interviews Tutorials Debates Group: Students are to work in groups of 5. Students will decide the members of their group. Please see the lecturer if you are having difficulty in this process. Each group is required to elect a project leader. The project leader is required to meet with the lecturer to discuss progress of the project as well as handle any administrative matters on behalf of the group. Important questions to ask yourselves What is the purpose of the podcast? Who is our desired audience? What topics/themes should be covered during the recording? Who will work on each portion of the podcast? Delinquency All members of the group are required to participate equally in this project. Students are encouraged to report any form of delinquency from their peers to their lecturer. All information will be kept strictly confidential. The lecturer will interview the delinquent student in question and, if found guilty, adequate deductions to the student’s mark will be made. Available resources: A camcorder is available for use. Your group will be held responsible for any damage to the camcorder and compensation will be sought via your caution money. 109 Projector Please consult with the lecturer as soon as possible if you require any additional resources. All attempts will be made to source them for you. Suggested programs to explore: Powerpoint Keynote Camtasia Final Cut Pro iMovie Blender YouTube Xtranormal Movie Maker http://www.youtube.com/create_detail/Xtranormal YouTube GoAnimate: http://www.youtube.com/create_detail/GoAnimate Acceptable video file formats: .AVI .WMV .MOV Please keep file size under 2GBs. Please consult with lecturer if you want to use another file format. Important dates: Date: Project leaders are to submit a Microsoft (MS) Word doc. containing the following information via a Turnitin link on myelearning o Working title of project (can be refined throughout the project) o Names and ID nos. of project leader and other members of the group o Contact information of project leader (cell, email) Date: At least 2 members of the group are to meet with the lecturer to discuss their project. o This should be a 15 min meeting. o You are required to discuss with the lecturer Your plans for this project Resources you will be using / require Date: Project leaders are to submit a Group project outline (MS Word doc) containing the following information via a Turnitin link on myelearning: 110 o Names and ID nos. of project leader and other members of the group o Working title of project o Synopsis Objectives Biochemical themes / topics covered (list them) Description of what you plan to do o Resources o Contribution of each member This exercise will be marked. Please see assessment section for details Date: Project leaders of each group are to update lecturer on progress of their project. This can be done via email, FB, Skype Date: Project leaders of each group are to update lecturer on progress of their project. This can be done via email, FB, Skype Date: Submit project to the Biochem Office Rm 217. Please sign the submission sheet provided. Submission: All files are to be submitted electronically on a CD or DVD. In addition to the video file you are required to submit a MS Word doc (or equivalent) indicating the following: Names and ID nos. of project leader and other members of the group Title of project Abstract (<250 words) Contribution of each member Please submit CD / DVD in a sealed envelope and submit to the Biochem Office Rm 217; Mon 12 th November 2012; 8am – 3:30pm. Please ask the secretary to stamp the date on your envelope before submitting and sign the submission sheet provided. One submission per group. Late Submission: 0.25% of the 10% will be deducted for each day late. Assessment: Project outlines and final submissions will be marked as a group, that is all members of a group will be given the same mark. However students found guilty of delinquency will be individually penalized. 111 Project Outline: ASSESSMENT CRITERIA EXCELLENT GOOD WEAK All components Formatting are present [5 mks] Date Page number [5] Headings and subheadings Names and ID#: Working title of project: Synopsis Objectives Biochemical themes / topics Procedure Resources Contribution of each member 1-2 components are absent All or most components missing [3 - 4] [0-2] All information is given concisely All information given but could have been summarized more Some information missing Content [10 mks] Biochemical themes and topics come from the BIOL 2365 syllabus Objectives and procedures give a clear direction for the project. Biochemical themes and topics come from the BIOL 2365 syllabus Some objectives and details of the procedure are vague Biochemical themes and topics are not related to the BIOL 2365 syllabus Objectives and details of procedure are vague [0-2] [7-10] Final submission: ASSESSMENT EXCELLENT CRITERIA Well articulated, Delivery animated, lively [5 mks] presentation, kept interest, well paced [3-6] GOOD WEAK Well articulated, rarely losing interest, few slips or pauses Poorly articulated with numerous slips or pauses 112 Abstract [10 mks] [5] [3 – 4.5] [1 – 2.5] Highlights the major points covered Highlights some of the points covered Highlights few points covered Concisely describes the content and scope of the video Partially describes the content and scope of the video Does not describe the content and scope of the video Adds no new information, but simply summarizes the information in the video Crucial keywords that describe the video are missing Crucial keywords that describe the paper are missing Exceeds the word limit Exceeds the word limit [8 -10] [5-7] [0-4] No errors in the information given Few errors in the information given Many errors in the information given Excellent understanding of the biochemical concepts discussed Good understanding of the biochemical concepts discussed Poor understanding of the biochemical concepts discussed No errors in spelling / pronunciation of common biochemical terms Few errors in spelling / pronunciation of common biochemical terms Many errors in spelling / pronunciation of common biochemical terms Ensures all the crucial keywords that describe the video appear in either the title or the abstract. Meets the word limit. Content [15 mks] [11 – 15] Creativity [10mks] [5-10] [0-5] Excellent use of technology Good use of technology Poor use of technology Presented content in a very creative manner Presented content in a fairly creative manner [5 – 7] Little or no creativity shown [8 – 10] [0– 4] 113 Length of video 15 mins [5mks] [5] Outline as well as video Time was submitted on time. management for duration of Met with lecturer on project stipulated times. [5 mks] [5] 16 - 20 mins > 5mins over or <13 mins [2.5 – 4.5] [1 – 2] Either outline or video was not submitted on time Missed most deadlines Did not meet lecturer on some of the stipulated times [2 – 4] [0 – 1] Assessment overview: Project outline: 15 mks Final submission: 50 mks Total: 65 mks This is worth 10% of your final mark. Copyright guidelines: These are some general guidelines to adhere to up to three minutes or 10 percent, whichever is less, of a single copyrighted motion media work. up to 10 percent or 1,000 words, whichever is less, of a single copyrighted work of text. an entire poem of less than 250 words or up to 250 words of a longer poem but no more than three poems by one poet or five poems by different poets from a single anthology. up to 30 seconds or 10 percent, whichever is less, of music and lyrics from a single musical work. up to five photographs or illustrations by one person and no more than 15 images or 10 percent, whichever is less, of the photographs or illustrations from a single published work. up to 2,500 fields or cell entries or 10 percent, whichever is less, from a numerical database or data table. YouTube: Please note that these videos will be posted on the BiochemJM channel for the general public to view and learn from. Please inform the lecturer if your group has any concerns about this. Please inform your lecturer of any concerns or queries you may have about this project. “The riskiest thing you can do in life is not to take chances. The safest thing you can do is be remarkable. Imagine, create, innovate.... Biochem JM” 114 THE UNIVERSITY OF THE WEST INDIES, ST. AUGUSTINE FACULTY OF SCIENCE AND TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE DOCUMENTATION COURSE CODE: BIOL2464 COURSE TITLE: Fundamentals of Ecology NO. OF CREDITS: 3 LEVEL: Undergraduate – Level 2 PREREQUISITES: (BIOL1262 Living Organisms I AND 6 credits from (BIOL 1263 Living Organisms II OR BIOL 1362 Biochemistry I OR BIOL 1364 Genetics I)) OR (ESST 1XX1 Biology for Environmental Sciences AND 6 credits from (ESST 1XX0 Physics for Environmental Sciences OR ESST 1XX2 Chemistry for Environmental Sciences OR ESST 1XX6 Human Impact on the Environment)). ANTI-REQUISITE: This course cannot be credited together with BIOL1462 General Ecology and Biometry ENROLMENT: Maximum 300 students COURSE DESCRIPTION: This course provides an introduction to ecology, the science of organisms and the environment, and gives the necessary background for advanced courses in ecology. The student will be introduced to the different levels of ecological organization and the types of studies associated with each. They will be introduced to the major factors that influence the distribution and abundance of organisms and appreciate the factors that influence the structure of ecological communities. The functioning of ecosystems in terms of the movement of energy and nutrients will also be covered. Apart from a general introduction to ecological fieldwork the students will be introduced to practical skills that will allow them to assess spatial distribution patterns of organisms and estimate their population sizes and population parameters. The students will investigate community structure and function using a variety of methods many of which involve fieldwork on campus. The course is organised into lectures and tutorials covering general and specific concepts in ecology. In tutorials students are expected to prepare, participate and perform in an active way in order to engage with the content. Assessment will be based on course tests, practical reports, and a final theory exam. COURSE RATIONALE: This course is one of the core level 2 courses for the major in Biology and the Biology degree, and for the Environmental Science degree. At the end of the course, students are expected to have an understanding of the fundamentals of ecology which is suitable for the biologist going on to specialize in other areas, or as the basis for further study for students specializing in ecology, environmental biology, or environmental science. INSTRUCTOR INFORMATION: Name of instructor: Dr A. Hailey (coordinator), Dr M. Oatham Office address and phone: Room 226, Zoology Section, New Wing, Natural Sciences Building. Phone ext 82206 [email protected] Email address: 115 Office hours: Monday, Wednesday, Thursday, 11-12 a.m., other times by appointment Preferred methods of contact: Email or myelearning Academic Forum Communication policy: Matters concerning the individual student should be raised by email, from the student’s UWI email account. Matters of interest to the class should be raised as Academic Forum postings on myelearning. In either case students can expect a response within 48 hours. DO NOT use myelearning messaging, which is only supported via email and after some delay. CONTENT: Ecology and its domain Geographic range habitat and niche, abiotic and biotic environment Ecological role of abiotic factors (climatic and edaphic) on plant and animal populations. Population performance along physical gradients Population structure and demography; population change over time, growth models, dispersal, life tables and resource allocation patterns Species interactions: competition, predation, herbivory, commensalism, amensalism, and mutualism Communities; community classification, concepts and attributes Island communities Primary and secondary ecological succession Nutrient cycling and energy flow Primary and secondary production, trophic levels and ecological efficiency GOALS/AIMS: This course aims to Introduce students to the major subject and growth areas of ecology Relate ecology to other aspects of biology, especially evolution, physiology, and biodiversity Give students an introduction to the scientific method, particularly the testing of hypotheses using quantitative data LEARNING OUTCOMES: At the end of the course, the student should be able to: Describe the levels of ecological organization and the types of studies associated with each level of organization Evaluate the major factors which affect the distribution and abundance of organisms Describe the factors which influence the structure of ecological communities Describe the functioning of ecosystems via movement of energy and nutrients Practical skills should include the ability to Assess spatial distribution patterns Estimate population sizes and population parameters Investigate community structure and function using a variety of methods, including field work ASSIGNMENTS: Practicals 6. Qualitative sampling of grassland plants 7. Population analysis of animals 8. Interactions between organisms: predation and competition 9. Quantitative sampling of grassland insects 116 10. Aripo Savanna plant communities Theory tests Six short theory tests, taken during tutorials and practicals. The tests will involve multiple choice, short answer, and notes formats. COURSE ASSESSMENT: Coursework practical: Coursework theory: Final examination: 25%, based on the best 4 of the 5 practical marks from the written reports and marks for participation. Due 7 days after the class. 25%, from the best 5 of the 6 marks from the theory tests. 50%. The examination is of 2 hours duration. It is based on essay questions, with two (2) questions to be completed, one from each section, from a choice of six (6). EVALUATION: Feedback on the course will be obtained informally from students on an ongoing basis by regular interactions and meetings among students, demonstrators, teaching assistants and the course lecturers in practicals and tutorials. Formal feedback will be via election of Class Representatives who sit on the Departmental Student-Staff Liaison Committee meetings held twice during the semester. Class reps will channel both concerns and commendations to the meeting as guided by the Department’s Standard Operating Procedures. Formal evaluation of the entire course will be accomplished via a UWI Course Evaluation questionnaire administered anonymously and confidentially at the end of the semester. All feedback will be considered on an ongoing basis and corrective action or adjustments made or discussed with students promptly or incorporated the following year. TEACHING STRATEGIES: Contact hours: 36 hours Lectures: 18 hours Tutorials: 6 hours Practicals: 12 hours (5 x 5 hours less 10 minutes = 24 hours) Lectures and tutorials are given to the whole class, and practicals are usually delivered to the students in three (3) streams. The course outline, objectives, and practical schedules are available on myelearning, together with the opportunity for feedback through the Academic Forum. Lecture materials are reduced to promote understanding of principles rather than transmission of facts, in particular with diagrams simplified to the essentials. Practicals build from initial qualitative observations on campus, through pre-set quantitative exercises and hypothesis testing using quantitative data, to a field exercise in an undisturbed habitat. Practical schedules are available before the class to maximise usefulness of the class time. RESOURCES: Essential Text 1. Mackenzie, A., Ball, A. S. & Virdee, S. R. 2001. Instant Notes in Ecology. 2nd ed. London: Taylor & Francis. Recommended Texts These texts should be used for supplementing the course text book, following up on lectures, and for research for practical reports. 1. Begon, M., Townsend, C. R. & Harper, J. L. 2005. Ecology: Individuals, Populations and Communities. 4th ed. Oxford: Blackwell. 117 2. Beeby, A. & Brennan, A. 2004. First Ecology. 2nd ed. Oxford University Press. 3. Brower, J., Zar, J. & von Ende, C. 1998. Field and Laboratory Methods for General Ecology. 4th ed. Boston: McGraw Hill. 4. Smith, T.M. & Smith, R.L. 2009. Elements of Ecology. 7th edition. San Francisco: Benjamin Cummings. COURSE CALENDAR: Week 1 2 3 4 5 6 Lectures and Tutorials Course Overview (Tutorial) Evolution and the niche Individual ecology Population: distribution Population: numbers and distribution Population: demography Population regulation Tutorial Interactions between species Tutorial Life history strategies Applied ecology: harvesting and species conservation Community: definitions and descriptions Community: diversity and stability Community change: succession Island community dynamics Practicals Assignments due 1. Qualitative sampling of grassland plants Practical 1 2. Population analysis of animals 3. Interactions between organisms Test 1 Practical 2 Test 2 Test 3 7 8 9 10 Ecosystem processes and components Tutorial Ecosystem: producers Ecosystem: consumers 11 Ecosystem: nutrient cycling Tutorial Practical 5 Test 5 12 Test 6 13 Applied ecology: biodiversity conservation Tutorial Course Review Practical 3 4. Quantitative sampling of grassland insects Practical 4 Test 4 5. Aripo Savanna plant communities 118 ADDITIONAL INFORMATION: Refer to the Life Sciences Undergraduate Handbook for general guidelines for teaching, assessment, student conduct, essential skills, and support at http://sta.uwi.edu/fst/lifesciences/documents/handbook.pdf . Refer to the General Information and General Regulations in the Faculty Booklet: http://sta.uwi.edu/resources/documents/facultybooklets/ScienceTechUndergrad.pdf. Students are reminded that they must attend a minimum of 75% of the practical sessions and tutorials. Failure to do so will result in debarment from the final examination. Students are hereby informed that plagiarism is forbidden and all unsupervised coursework items must be accompanied by a Coursework Accountability Statement in order to be assessed. Specific items may require submission through Turnitin on myeLearning. Refer to ‘University Regulations on Plagiarism’ available from http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf HOW TO STUDY FOR THIS COURSE: Students should attend all lectures, tutorials, and practicals. The lectures are designed to cover the most important points in each subject area, in the opinion of the lecturer, and are condensed from a much greater volume of material. Students who do not attend lectures miss the benefit of this work by the lecturer, and have to review the textbooks themselves. Although less satisfactory than attending lectures, independent study has been made easier by detailed learning objectives cross-referenced to sections of the textbook. Practical instructions are provided on myelearning well before the start of the class, and should be read before attending the class to make full benefit of the class time. Tutorials are designed to help students with aspects that have proved difficult in the past, rather than providing new material. Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 119 THE UNIVERSITY OF THE WEST INDIES, ST. AUGUSTINE FACULTY OF SCIENCE & TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE DESCRIPTION COURSE CODE: BIOL 2764 COURSE TITLE: SEMESTER: Physiology of Plants 2 LEVEL: 2 NO OF CREDITS: 3 PRE-REQUISITES: BIOL1262 - Living Organisms I or BIOL1261 Diversity of Organisms or (BIOL1065 Diversity of Plants and Animals and AGRI1012 Microbiology) and either BIOL1364 Genetics I and BIOL 1362 Biochemistry I or BIOL1061 Cell Biology and Genetics ANTI-REQUISITE: BIOL2761 Plant Physiology The first part of the course provides the essential concepts of plant physiology with comprehensive coverage of water relations, mineral uptake, and photosynthesis. The second part of the course explores how these resources are translated into plant growth and provides an introduction to how plants respond to environmental signals at the whole plant level. PURPOSE OF THE COURSE/COURSE RATIONALE The course provides an understanding of how plants gain the resources need for growth and survival. This information is essential to understanding the functioning of ecosystems and the application of plant biology. Within the Life Sciences department it will be a core module for the Biology Degree/Biology major. INSTRUCTOR’S INFORMATION Name of course coordinator: Aidan D. Farrell 120 Office address and phone: 220, Zoology Office, Natural Sciences Building, New Wing Natural Sciences Building Email address: Office hours: [email protected] 9:00 - 5:00 daily (email for appointment) Preferred method of contact: Email Communication policy: Students should use their UWI email account for communication and can expect a response within 48 hours CONTENT Plant Physiology focuses on how plants gain the resources needed for growth. There is detailed coverage of the key mechanisms at the whole plant level. The course begins with a detailed description of how plants acquire water, carbon and nutrients. This is followed by an examination of how these resources are utilised for growth. The remainder of the course looks in detail at examples of how plants use extrinsic signals to respond to their environment. Topics are divided as follows: Plant anatomy review Plant water relations Photosynthesis & translocation Membranes and mineral uptake Cell expansion & plant growth Phytochrome Plant growth regulators Nastic and Tropic movements Control of flowering GOALS/AIMS This course aims to: Review the essential concepts in whole plant physiology Provide students with the knowledge needed to analyses and evaluate physiological process at the whole plant level Enable students to describe the role of these physiological processes in ecosystems Enable students to develop skills in problem solving and interpretation through ‘first-hand’ experience of how physiological mechanisms are discovered LEARNING OUTCOMES At the end of this course students should be able to: Describe how plants acquire the resources needed for growth Differentiate between different mechanisms for resource acquisition Recognise the general system of signal perception, transduction and response in plants Describe and contrast specific examples plants responding to extrinsic signals Interpret experimental evidence relating to plant physiology 121 COURSE ASSESSMENT Students are required to achieve a grade of 40% overall, irrespective of their performance in individual components. Component % Final Grade Description Final exam 50% 2-hour written exam with 20 multiple choice questions, and a choice of 2 out of 4 essay questions In-course test 1 15% Resource acquisition: multiple choice and short structured questions In-course test 2 15% Growth and responses to environment: multiple choice and short structured questions Practicals 20% Performance and reporting of practical exercises TEACHING STRATEGIES Contact hours (36 credit hours): Lectures: 18 h Tutorials: 6 h Practicals: 12 hours (4 x 6 hours = 24 hours) Lectures: Lectures will provide valuable synthesis and evaluation of the growing body of available information, and prioritise content relevant to course assessment. Practicals: Fortnightly practicals will provide hands on experience for students to gain skills required for conducting well designed laboratory sampling and experiments; to problem solve and trouble shoot in real-life situations; and to become familiar with performing and interpreting physiological experiments. Performance in the practicals will be assessed as well as reports based on the practical activities. Tutorials: Tutorials will cover course topics in a highly interactive format using a variety of collaborative active learning techniques. Myelearning: MyeLearning, will be used extensively during this course for communication among students and staff (email, discussions) official posting of important notices (coursework assessment notices, instructions, and in-course results) provision of course details, lecture notes, practical guides, tutorial briefings provision of recommended resource materials and links to resources on specific websites RESOURCES 122 Many resources are available via MyeLearning including: Lecture presentations (including learning objectives) ‘General Resources’- direct links to videos, web-tools and publications relevant to the course Discussion forums Essential texts: Plant Physiology. Taiz and Zeiger. 2010 (Sinauer Associates) (In Reserve Section, UWI, St Augustine, Main Library) Texts for further reading: Introduction to Plant Physiology. Hopkins & Huner. 2008 (John Wiley & Sons) Plant Physiology. Salisbury and Ross. 1992 (Wadsworth) Lehninger Principles of Biochemistry. Nelson & Cox. 2008 (Freeman) Biochemistry & Molecular Biology of Plants. 2002. Buchanan et al. (Wiley) Web links: http://5e.plantphys.net/ http://plantphys.info/plant_physiology/schedule.shtml http://plantsinaction.science.uq.edu.au/edition1/?q=content/contents-page Practical equipment: All equipment will be provided by the Dept. Life Sciences, except the Infrared Gas Analyser which will be demonstrated by the Dept. of Food Production Glasshouse space for growing and treating plants. Psychrometer, Porometer, Pressure chamber, Infrared Gas Analyser, Variable light rigs, Darkroom, light filters. Light microscopes, Micro balances, Drying ovens, Microwave ovens, Cork-borers, Razor blades, glassware, etc. 123 COURSE CALENDAR Week Lecture 1 Introduction Plant Anatomy Review 2 Water Relations Water Relations 3 SPAC Stomata & Transpiration 4 Translocation & Partitioning Photosynthesis: light reactions 5 Photosynthesis: carbon reactions Photosynthesis: whole plant 6 Tutorial In course test I 7 Phytochrome Plant Growth Regulators 8 Cell expansion and Plant growth Cell expansion and Plant growth 9 Membranes / Ion uptake Mineral Nutrition 10 Nastic & Tropic Movements Lab/Tutorial Deadlines Water Relations Water Relations Practical report Gas Exchange Gas Exchange Practical report In course test I Germination, Nutrition & Hormones (Part A) Practical report Germination, Nutrition & Hormones (Part B) Practical report Control of flowering 11 12 Tutorial In course test II In course test II Tutorial Tutorial 124 ADDITIONAL INFORMATION Refer to the Life Sciences Undergraduate Handbook for general guidelines for teaching, assessment, student conduct, essential skills, and support at http://sta.uwi.edu/fst/lifesciences/documents/handbook.pdf . Refer to the General Information and General Regulations in the Faculty Booklet available from http://sta.uwi.edu/resources/documents/facultybooklets/ScienceTechUndergrad.pdf . Students are reminded that they must attend a minimum of 75% of the practical sessions and tutorials. Failure to do so will result in debarment from the final examination. As a ge eral pri iple, edi als or other e uses a o l e use a stude t s prese e at a assig ed ti e. “tude ts must still complete the assigned work (make-up lab report or make-up test) in order to obtain the marks for that item of coursework. The student is responsible for liaising with the Course Coordinator or Teaching Assistants to ensure the assigned make-up is completed. Students are hereby informed that plagiarism is forbidden and all unsupervised coursework items must be accompanied by a Coursework Accountability Statement in order to be assessed. Specific items may require su issio through Tur iti o eLear i g. ‘efer to U i ersit ‘egulatio s o Plagiaris a aila le fro http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf HOW TO STUDY FOR THIS COURSE: Attendance is mandatory for lectures, tutorials and practicals. Prior preparation is strongly advised to able to fully participate in activities and obtain the full value of the sessions. Thorough use should be made of the resources provided and students are strongly advised to become familiar with them and start utilising them from the first week. Regular updates on course progress and materials are also highly recommended and you should check into you r UWI email and myelearning on a frequent regular basis to review materials, assignments and activities. Students are encouraged to interact regularly with staff on their projects, even outside of the assigned tutorial times to ensure prompt, satisfactory solution of any problems and to monitor progress. Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 125 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 THE UNIVERSITY OF THE WEST INDIES ST. AUGUSTINE FACULTY OF SCIENCE AND AGRICULTURE DEPARTMENT OF LIFE SCIENCES TITLE: BIOL2867 Physiology of Animals CREDITS: 3 LEVEL: Undergraduate-Year II SEMESTER: II PRE-REQUISITES: BIOL1263 Living Organisms II and BIOL1362 Biochemistry I and either BIOL1364 Genetics I or ESST 1001 Biology for Environmental Sciences ANTI-REQUISITES: BIOL2862 Animal Physiology OFFERING DEPARTMENT: Department of Life Sciences, Faculty of Science and Agriculture, The University of the West Indies, St. Augustine. COURSE COVERAGE: Physiology of Animals is the study of how animals’ function. It is a broad-based course which provides an introduction to molecular and cellular physiology and the principal physiological systems in animals, and how these systems function to maintain homeostasis in various environments. It covers fundamental concepts in osmoregulation and excretion, neurophysiology, muscle physiology, respiration, thermo-physiology, circulation and gas transport, endocrinology, and cardiovascular physiology. We also look at some of the major stressors on physiological processes and show how animals have been able to deal them. Typical stressors that are covered include osmotic pressures, water limitation, hypoxia, altitude, depth, temperature extremes and exercise. While 126 animal physiology examines systems and processes common to all animal species, this course will focus on vertebrates, with a special emphasis on mammalian systems. SIGNIFICANCE/RATIONALE: The physiology of animals is a study of how animal function and respond to changes in their environment. This course allows us to understand the basic physiological functioning of animals and how these allow animals to deal with stressors in their environments. Knowledge of certain aspects of the natural history, morphology, behaviour, and environment of an animal is necessary to fully appreciate the importance of its physiology. ENROLMENT CAPACITY: 200 students (maximum) PURPOSE OF THE COURSE This course provides a basic understanding of the physiological functioning of animals. This discipline is principally concerned with how animals are physiologically adapted to the environment in which they live. INSTRUCTOR’S INFORMATION Dr Azad Mohammed (Coordinator) Room 222, Natural Sciences Building, UWI- Life Sciences Phone: 868-662-2002 Extn. 82046; Fax: 868-663-5241 Email: [email protected] Preferred contact – By Email Professor John Agard Room 220, Natural Sciences Building, UWI- Life Sciences Phone: 868-662-2002 Extn. 83095; Fax: 868-663-5241 Email: [email protected] Preferred contact – By Email Office-Contact hours (Coordinator) Day Monday Tuesday Wednesday Thursday Friday Time 8-10AM 8-10AM 8-10AM 8-10AM 8-10AM LETTER TO THE STUDENT Dear Students, I would like to extent a very warm welcome to you as we get prepared for the start of BIOL xxxx - Physiology of Animal. I am Dr Azad Mohammed, your Course Coordinator. Part of this course is also being taught by 127 Professor John Agard. This course is fully supported through myelearning, which we update regularly. Please feel free to contact me (course teacher)/TA/demonstrator for any academic help and assistance. Please know that we are here to support your learning and success as a student in FSA. This course is designed to provide you with an understanding of the basic principles of comparative physiology and their ecological and evolutionary significance. Another goal of the course is to teach students how to critically investigate physiological problems experimentally in relation to an animal’s environment and the strategies used to solve these problems. A few points to note: The Course Schedule (dates/activities) is available on the course page and should be downloaded for your reference and continued guidance, as soon as you have access. Please take the time to read the policy on plagiarism. Once again, I would like to take this opportunity to welcome you and look forward to some healthy interactions over the course of the next semester. Your Course Coordinator Dr Azad Mohammed. ‘there are no strangers in this world, only friends you haven’t yet met’ CONTENT The course covers several major physiological systems in the body. It explores the structure and function of animals and allows students to gain some knowledge on the diversity, as well as similarities in the physiology of animal - at the microscopic as well as macroscopic level. Respiratory Physiology The initial part of the course looks at respiratory systems and explains how these have become modifies in different groups of organisms allowing them to live in specific environments. The respiratory systems of airbreathing vertebrates, the gill system of fish, the insect tracheal system, and the unidirectional gas exchange system of birds It also looks at the principles of gas exchange and shows how the respiratory gasses are transported to and away from tissues via the circulatory system. It also looks at the adaptations of these systems under extreme conditions. Osmoregulation One of the main challenges that organisms face is maintenance of internal homeostatic conditions. this sections looks specifically at the process of regulating water potential in order to keep fluid and electrolyte balance within a cell or organism relative to the surrounding. It is the way by which an organism maintains suitable concentration of solutes and amount of water in the body fluids. Thermal Physiology 128 Thermo regulation is another mechanism used to homeostatic conditions of tissues. This topic will look specifically at temperature regulations and the strategies most organism use to deal with changing ambient environmental conditions. Neurophysiology Sensory and neuro physiology covers the broad range of topic specifically looking at how stimuli are integrated, transmitted and interpreted by the body. We examine the structure of the neuron and how it is able to achieve its function. Nueromuscular physiology Neuromuscular Physiology can be studied at the most simple levels by using the classical models of nerve signaling to muscles through acetylcholine receptors. This topic shall investigate the structure and function of the neuromuscular junction and the sequence of events that occur following stimulation of the muscle to the eventual contraction. Digestion Digestive physiology is concerned with the tissues that contribute to the physical and chemical breakdown of food. It would focus on the chemical processes that break food into forms that can be transported and metabolized into other molecules. COURSE GOALS: The main goal of the course is to familiarize students with the principles of physiology and its ecological and evolutionary significance. Another goal of the course is to teach students how to critically investigate physiological problems experimentally in relation to an animal’s environment and the strategies used to solve these problems. COURSE OBJECTIVES: At the end of the course, the student should be able to have a good understanding of the following: The structure and functions of the major physiological systems found in vertebrate and invertebrate organisms and how these systems integrate in the whole animal. a. The respiratory systems (ventilation, diffusion and perfusion of blood) of air-breathing vertebrates, the gill system of teleost fish, the insect tracheal system, and the unidirectional gas exchange system of birds. b. The function of the nervous system including synaptic transmission and sensory perception in vertebrates and invertebrates c. The skeletal muscle system and the physiology of muscle contraction in response to stimulation. The basic principles of physiological regulation and homeostasis, a. How organism are able to maintain normal homeostatic function under extreme temperature changes, and mechanisms for adaptation. b. Osmotic regulation and ionic balance AIMS/GOALS At the end of the course you should • have a thorough knowledge and understanding of many aspects of animal physiology based on 129 attendance at lectures and reference to the recommended textbook • be able to perform and write-up experiments in animal physiology having done the practicals and obtained feedback from marked work • be able to assess your knowledge of animal physiology in preparation for the exams. LEARNING OUTCOMES At the end of this course students will be able to: record and interpret physiological phenomena in appropriate units. analyze and interpret laboratory data using graphical and statistical tools. investigate physiological phenomena using appropriate lab methods. search the library and internet to obtain literature pertinent to a chosen physiological topic. actively seek and assimilate information from texts, assigned articles and reference texts. work collaboratively with one or more lab partners. Dispositional outcomes: Students will understand the relevance and importance of physiology in the Major of their degree. Students will not hesitate to ask questions or seek help from the instructors when material is difficult or confusing. Mode of Delivery: Lectures -24 hours: Didactic; interactive Tutorials - 6 hours: Interactive; mind maps; problem-solving Laboratory classes- 20 hours (4 Interactive practical tasks; problem-solving five-hour sessions): COURSE ASSESSMENT Assessment would be based on a combination of in-course assessment (50%) and final examination (50%). In-course assessments consist of both theory and practical components, and together make up the 50% of the final marks. A student missing an in-course examination because of an illness or legitimate emergency will be allowed to do a makeup examination the date of which will be advertised and shall be within 7 days of the first test. In such a circumstance, the student should make every reasonable attempt to contact the instructors as soon as possible. While make-up exams will cover the same content area as a missed exam, the exam format and specific questions may be different. There shall be no makeup labs, students missing a practical exercise must submit a valid medical within 7 days of the practical. Failure to do so will mean that a grade of zero will be given for that practical. Those who submit a valid medical will be given a final practical mark that is an average of all their marks. Any student missing more than 25% of practical classes or tutorial sessions without a medical or other valid excuse can be debarred from writing the final exam. Final exam: Duration 2 hrs; Essay and short answer questions. The final paper may include a compulsory section. The contribution of each component to the final grades is as follows: 130 EVALUATION Course Work: 50% - Writing across the curriculum exercises: Laboratory reports (10 x 2% ): In-course test 1: In-course test 2: Tutorial Attendance and participation: Final examination (2 hours) 5% 20% 10% 10% 5% 50 % Coursework in BIOL2XXX will be assessed in the following ways: 1. Writing across curriculum exercises-5%. These activities are designed to encourage students to become active learners by engaging knowledge to further develop understanding. The exercises would help students understanding content and expressing ideas. A total of five simple exercises worth a total of 5 marks (1 mark per exercise) would be given during lectures or tutorials (no more than one per week). Each exercise would take between 5 – 10 minutes to complete and would comprise of varying activities including: a. Focused free-writing: Students may be asked to briefly write on their understanding or perspective on a subject/topic covered or on how the subject/topic may be connected to other situations. b. Entry slips-: Short responses to questions posed at the beginning of class; c. Answering questions: One or few questions would be given which students must answer and submit for grading. d. Short summary: Students are asked to summarize main points of a reading assignment in a short paragraph. e. Group presentation: The tutorial class would be divided into groups and each group would be required to make a short presentation on a document provided. 2. Class participation- 5% Student participation in tutorial sessions will be monitored by the tutor and a grade assigned at the end of the semester. Participation would include more than just showing up at tutorial – some evidence of an active role in the tutorial discussion would be required to obtain the full 5% for participation. At the beginning of the semester, the students in each tutorial would engage in developing the exact assessment criteria for this assignment, and the student-developed criteria will be used to determine each student’s participation grade. 3. In-course Test-20% Two in-course tests would be given worth 20%. The test would be given in weeks 6 and 12 of the semester and would comprise MCQs and short answer questions. 4. Practicals (20%): Practicals will contribute 20% to the final grade. There will be 4 practical sessions for the duration of the course. The final mark for each practical will consist of the final report (70%) and a performance mark (30%). Late lab submission would be penalized by a 15% deduction of the final grade for each day late. 5. Final examination (60%) 131 The final paper would consist of a 2hr examination which includes a compulsory section. It would have a mixture of short answer and essay type questions. COURSE ASSESSMENT EVALUATION BIOLxxxx will be evaluated in two ways – (a) through the Class Representative and the Life Sciences StudentStaff Liaison Committee, and (b) an end of semester course evaluation survey. Feedback will be obtained informally from students on an ongoing basis by regular interactions and meetings among students, demonstrators, teaching assistants and the Course Coordinator in practicals and tutorials. Formal feedback will be via Class Representatives who sit on the Departmental Student-Staff Liaison Committee meetings held twice during the semester. Class reps will report concerns and commendations to the meeting as guided by the Department’s Standard Operating Procedures. Formal evaluation of the entire course will be accomplished via a UWI Course Evaluation questionnaire administered anonymously and confidentially at the end of the semester. All feedback will be considered on an ongoing basis and corrective action or adjustments made or discussed with students promptly or incorporated the following year. TEACHING STRATEGIES: Course objectives will be achieved through the aid of the following learning activities: 1. 2. 3. 4. Assigned readings Class lecture and videos Web page resources Practical exercises The material presented in all modes of instruction will be sufficient in content to allow students to have a good understanding of the topics. Lectures will help to direct the students to some of the more important details of each topic. Assigned readings will serve to supplement lectures. These include lectures notes, web based materials and original research papers on topics covered. We would also request that students post links to interesting articles for discussion in the forums in the course page. RESOURCES Text books: Principles of Animal Physiology 2nd Edition Christopher D. Moyes and Patricia M. Schulte. OTHER RESOURCES: Discussion topics will be placed online for class comments. READINGS 132 Instructors will periodically upload in my-elearning important research articles, review papers, book chapters and other related study materials. Students are expected to consult prescribed text books for relevant chapters for complete details and for preparation for examinations Students are expected to download those files or visit the suggested websites or read relevant book chapters/journal articles before they come for the following class. These were considered and included as portions of the syllabus and covered fully during examinations. BIOL 2862 COURSE SCHEDULE WEEK LECTURES 1 1. 2. 2 3. 4. 3 4 Regulation of heartbeat (Azad Mohammed) 6. Regulation of heartbeat (Azad Mohammed) 7. Cardiovascular responses to diving in mammals and birds (Azad Mohammed) Cardiovascular responses to high altitude (Azad Mohammed) Tutorial (Azad Mohammed) 9. 5 Properties of air and water as respiratory media. Comparative respiration and gas exchange efficiency of integuments, gills, mammalian lungs and bird lungs (John Agard) Comparative respiration and gas exchange efficiency (cont’d) (John Agard) Respiratory pigments and the factors that 1 The effects of oxygen and carbon modify their activity (Azad Mohammed) dioxide concentration on respiration in insects. Gas transport and acid-base balance of the Objective: To understand what is the blood. (Azad Mohammed) primary stimulus for respiration. 5. 8. PRACTICALS 10. Q10 and thermal increment. Ectothermy; the zone of tolerance, adaptations to cold and heat (John Agard) 11. Endothermy, the thermo neutral zone, responses to heat and cold. Heterothermy: torpor and hibernation. (John Agard) 12. Tutorial (John Agard) 2 The effect of temperature on the heart rate of an ectotherm. Objective: to understand how temperature affects Q10 in ectotherms 133 6 13. 14. 15. 7 8 Coursework exam 1 (Azad Mohammed) 3 Cardiovascular and respiratory physiology of humans Excretory systems (Azad Mohammed) Objective: Give students an idea of how cardiovascular physiology Water and osmotic regulation. Ionic changes to meet the demands of regulation (Azad Mohammed) environmental conditions. 16. Digestion We would investigate the tissues that contribute to the physical and chemical breakdown of food. Focus would also be on the chemical processes that break food into forms that can be transported and metabolized into other molecules (Azad Mohammed) 17. Digestion We would investigate the tissues that contribute to the physical and chemical breakdown of food. Focus would also be on the chemical processes that break food into forms that can be transported and metabolized into other molecules (Azad Mohammed) 18. Tutorial (Azad Mohammed) 19. The ultra structure of muscles (Azad 4. The Physiology of skeletal Mohammed) muscles. Objectives: The regulation of muscle contractions (Azad - Name and describe the phases of a Mohammed) muscle twitch - Explain why the “all or none” law is reflected in the activity of a single muscle cell, but not in an intact skeletal muscle. - Understand that the graded response of skeletal muscles is a function of the number of muscle fibers stimulated and the frequency of the stimulus. - Define and explain the physiological basis of the following; Subminimal, minimal, maximal and supramaximal stimuli Latent period Wave summation Tetanus Muscle fatigue 20. 134 Explain the effect of load on skeletal muscle 9 10 11 12 21. The mechanics of muscle contraction (Azad Mohammed) 22. The propagation and transmission of action potentials (John Agard) 23. Tutorial (John Agard) 24. The synapse; excitation and inhibition (John Agard) 25. The synapse; neuronal integration (John Presentation of labs (5%) Agard) 26. Tutorial (John Agard) 27. The organization of sensory systems (John Agard) 28. Transmission and sorting information (John Agard) 29. Tutorial (Azad Mohammed) 30. Coursework exam II (Azad Mohammed) of sensory - ADDITIONAL INFORMATION Students must attend 75% of their practicals. Failure to do so may result in the student being debarred from the final examination. (Examination regulations Section II part 19 http://sta.uwi.edu/resources/documents/Exam_and_GPA_regulations.pdf). In order to pass the course, you must gain a overall mark of 40% from both the coursework and the final theory exam. Any student who misses more than 25% of practical classes or tutorial sessions without a medical or other valid excuse can be debarred from writing the final exam. If you do have a disability and have not disclosed the nature of your disability and the support you need, you are invited to contact the Academic Support/Disabilities Liaison Unit (ASDLU) of The University of the West Indies (UWI), St. Augustine Campus. Phone: 662-2002 extension 83866, 83921, 83923 and 84254 Fax: 662-2002 extension 83922 Email: [email protected] http://sta.uwi.edu/asdlu/forms.asp Additional information posted in the Department of Life Science hand book may be consulted for additional details regarding, Role of Lectures, Tutorials and Practical’s, Attendance, Online Teaching Resources, 135 General safety guidelines in Lab and General Safety Practices Accident and incident reporting forms. Academic Dishonesty: Academic dishonesty, which includes plagiarism and cheating, is an extremely serious academic offense and carries penalties varying from failure in an assignment to possible suspension from the University. http://sta.uwi.edu/fsa/lifesciences/accountability.pdf (Department of Life Sciences Handbook) HOW TO STUDY FOR THIS COURSE The lectures will be delivered using power point presentation and slides/videos depicting real-time scenarios and thoroughly discussed. These will be posted on myelearning) the day after the lecture. Students should read all the relevant materials given. They should also attempt past paper questions which we can review. Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 April 2012 136 THE UNIVERSITY OF THE WEST INDIES, ST. AUGUSTINE FACULTY OF SCIENCE & TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE DESCRIPTION COURSE CODE: BIOL3063 COURSE TITLE: SEMESTER: Marine Ecology and Oceanography 2 LEVEL: 2 NO OF CREDITS: 3 Pre-requisites: Either BIOL1262 Living Organisms I and BIOL1263 Living Organisms II or BIOL1261 Diversity of Organisms or (BIOL1065 Diversity of Plants and Animals AND AGRI1012 Microbiology) and either BIOL2XXX Fundamentals of Ecology OR BIOL1462 General Ecology and Biometry Anti-requisites: BIOL2063 Marine Ecology COURSE DESCRIPTION: This course is an introduction to marine ecology and related aspects of oceanography and marine biology. It emphasizes ecological processes and adaptations that act to structure marine associations and permit their persistence through time. Lectures provide an overview of characteristics, biodiversity and ecology of these marine ecosystems. They will also highlight concepts, ideas and hypotheses of how marine ecosystems function. These principles are examined on a global oceanographic scale. The course will also explore the structure and function of marine communities and ecosystems; threats to marine ecosystems and include management strategies to provide sustainable benefits both to the ecosystems and human wellbeing. Students are expected to have a basic foundation in ecology and biodiversity. In addition to providing a foundation of theoretical knowledge, this course will emphasise practical skills and expose students to field and laboratory approaches for studying freshwater systems. It is an interactive ‘hands-on’ course where students are expected to prepare, participate and perform in an active way to engage with the content in a variety of ways. Assessment is designed to encourage students to work continuously with the course materials, explore and critically analyse research in this rapidly developing field. PURPOSE OF THE COURSE/RATIONALE: This is a core course for the Ecology & Environmental Biology minor in the Department of Life Sciences and is an essential area in environmental sciences, environmental biology and environmental management. It also broadens the scope of studies in biology, plant biology and zoology. 137 Marine ecosystems systems are diverse environments that support unique communities of organisms and provide invaluable ecosystem services for human survival and well-being. At the same time they are heavily impacted by humans since they integrate all activities on land, in the water and even the atmosphere. An appreciation of these systems is necessary for their effective management. INSTRUCTOR INFORMATION: Name of course co-ordinator: Dr. Judith Gobin Office address and phone: Room 222, Natural Sciences Building, UWI- Life Sciences, 868-662-2002 Extn. 82046; Fax: 868-663-5241. Email: [email protected] , Preferred contact – By Email Office hours: Mondays 12.00 to 2.00, Wednesdays 12.00 to 2.00 LETTER TO STUDENTS: Dear Students, I would like to extend a very warm welcome to you as we get prepared for the start of BIOL 3XXX – Marine Ecology & Oceanography. I am Dr Judith Gobin, your Course Coordinator. Part of this course is also being taught by Professor John Agard. This course is fully supported through myelearning, which we update regularly. Please feel free to contact me (course teacher), your TA or demonstrator for any academic help and assistance. Please know that we are here to support your learning and success as a student in FSA. This course is designed to provide you with an understanding of the main marine ecosystems and their ecological significance. Another goal of the course is to teach students how to critically investigate the marine environment in terms of communities of organisms and water quality. A few points to note: The Course Schedule (dates/activities) is available on the course page and should be downloaded for your reference and continued guidance, as soon as you have access. The major Field trip is on-board a Research Vessel and this trip is mandatory. Students do not necessarily have to be good swimmers (although this would be great!) since we have strict regulations with respect to our safety at sea. Please take the time to read the UWI’s policy on plagiarism. Once again, I would like to take this opportunity to welcome you and look forward to some healthy interactions over the course of the next semester. So, let’s get your feet wet! Your Course Coordinator Dr Judith Gobin. 138 CONTENT: Topics will include: Introduction to the Marine Environment and the different marine ecosystems. Properties of seawater, some basic oceanography and some basic ecological principles. Phytoplankton communities, shallow-water subtidal benthic associations, intertidal ecology, estuaries and salt marshes, tropical communities (coral reefs and mangroves), oceanic nekton and the deep sea environment. Lectures also cover the different adaptations by organisms for living in the different environments. Relevant examples are introduced from both tropical (including local to Trinidad and Tobago and the Caribbean) and temperate systems COURSE GOALS: This course aims to: Provide students with an introduction to marine ecology and related aspects of oceanography and marine biology. Introduce students to ecological processes and adaptations that act to structure marine associations and permit their persistence through time. Provide lectures and information which highlight concepts, ideas and hypotheses of how marine ecosystems function. Include a survey of local marine habitats and organisms and examine principles on a global oceanographic scale. GENERAL OBJECTIVES: The general objectives of the course are to: Provide course materials, activities and assessment using both face-to-face and online modalities Facilitate opportunities for students to engage with general concepts and specific examples to develop a comprehensive understanding of the nature of marine ecosystems and their biodiversity Allow students to observe, analyze and recognize the marine ecological communities of a local marine habitat in Trinidad and Tobago; Allow students to carry out ecological surveys including evaluations to determine environmental impacts on marine communities. Guide students in critically analysing and exploring questions in marine ecology and oceanography. Facilitate discussion on the management of marine ecosystems and their impacts on human well being. 139 LEARNING OUTCOMES: Upon successful completion of this course, the student will be able to: 1. Describe the ocean environment in terms of geology, geography, chemistry and water movement; 2. Identify the major taxonomic groups living in the ocean; 3. Critically evaluate the main hypotheses explaining how marine ecosystems function; 4. Describe the major ecosystems of the ocean in terms of physical and biological characteristics; 5. Analyze the marine environment and marine species' adaptations to the most important environmental parameters; 6. Observe, analyze and identify the marine ecological communities of Trinidad and Tobago; 7. Carry out ecological surveys to determine environmental impacts on marine communities. COURSE ASSESSMENT: Assessment will be based on a student’s final mark from the coursework components below. Component Final exam In-course tests % Final Grade 50% 20% Tutorials, online activities Practicals 10% 20% Description 2-hour written exam to answer 2 essay questions 2 tests (each 10%). The 1st- a Research Essay and the 2nd a multiple choice, true/false, short response question paper. Performance in interactive discussions and activities face-to-face and online Performance and reporting of 5 practical exercises ASSIGNMENTS: Students will also have two in-course tests, each covering specific areas of the course. These are designed to determine if the students can correctly recall all that was covered in the previous weeks of the course. The 1 st is an 1 Essay question and the 2nd is a multiple-choice question exam. (together total 20%) 140 Students will have to submit a written report for each of four practical exercises. These are designed to relate to all of the major topics that are covered in the lectures. The practical assessment will consist of four (5hr) Laboratory exercises plus 2 Journals associated with the Labs. Together these account for 20% of the final grade. Practical write-ups are handed in at the end of each lab. (6.00pm) Journals are handed in 1 week after the Field trip. (4.00pm) EVALUATION: At the onset- students are asked to pop in to see the Course Co-ordinator or to make an appointment- to address any problems they may be having. Both Dr. Gobin and Prof. Agard have an open-door policy (once they are on-campus and available). Chosen Class Representatives will attend meetings with the staff/student Liaison Committee, and give their feedbacks/comments on the course to the committee. Class Reps. are advised to address issues to me directly as soon as possible since there are only 2 meetings of this Committee per semester. This means issues may get delayed in being addressed. The UWI/IDU course evaluation forms at the end of the semester will be used to address and improve on the specific comments/feedback as best as possibly. TEACHING Strategies: Credits: 3 computed as follows: Lectures: 23 h Tutorials: 6 h Total = 2.4 cr Practicals: = 20h (0.8) Course objectives will be achieved through the aid of the following learning activities: 1. Class lecture and videos 5. 6. 7. 8. Assigned readings Web page resources including published scientific papers Field exercises Practical exercises (laboratory) 141 The material presented in all modes of instruction will be sufficient in content to allow students to have a good understanding of the topics. Class lectures will help to focus the student to some of the more important details of each topic. Field e er ises ill e pose stude ts to the sampling in the marine environment. ari e e iro e t a d allo the ha ds-o e perie e at Writing up the Field journal- gives students the opportunity to observe, note and process Field and sampling information before returning to the laboratory. Laboratory exercises will allow students to calculate, evaluate and synthesise the information and details gathered in the field. Assigned readings will complement lectures. These include additional lectures notes, web based materials and original research papers on topics covered during lectures and practicals. RESOURCES: myeLearning: The online teaching tool, myeLearning, will be used extensively during this course for communication among students and staff (email, discussions), official posting of important notices (coursework assessment notices, instructions, and in-course results), provision of course details, lecture notes, practical guides, tutorial briefings and provision of recommended resource materials and links to resources on specific websites. Tutorials may have specific supporting resources particularly on skills development. Essential and Recommended texts are in the Reserve Section, UWI Main Library or via links in the course outline and myeLearning Text books: Marine Biology. James W. Nybakken. 5th/6th Editions. OTHER RESOURCES: myeLearning (through my.uwi.edu) will be the main online resource. Discussion topics will be placed online for class comments. There is also a Facebook page- UWI Marine Ecology. 142 COURSE CALENDAR: WEEK LECTURES 1 Introduction to the # 1 Introduction to Course Field samplingmethodology, Bathymetry and instruments & topography of the Physical and Chemical ocean floor: major Properties of subdivisions of the Seawater. world ocean 2 Sea floor spreading, Tutorial 1 plate tectonics and continental drift 3 Atmospheric # 2 Sea/coastal area Circulation Oceanic trip Circulation 4 Primary Production The Classic Model of the Ocean Ecosystem: zooplankton grazing, PRACTICALS Assignments DVD/Blue PlanetPlankton & Tutorial 2 A changing Model of the Ocean Ecosystem: microorganisms, nanoplankton, bacteria and the marine planktonic food web 5 Soft bottom Benthic # 3 Sorting & ecology: Macrofauna Identification and analysis of plankton Meiofauna and benthic fauna from sea trip Sea/coastal area trip. Report write-up 143 WEEK LECTURES PRACTICALS 6 Rocky Shores DVD/Blue Planet- Test 1 Coastal seas & Tutorial 3 Sandy Shores 7 Esutaries Mudflats Marshes and Assignments # 4 East Coast rocky shore and/or sandy Salt shore (beach profiles), or mud-flat. Field tripincludes journal writeup. 8 Mangrove Swamps DVD/Blue Planetand wetlands Seagrasses & Tutorial 4 Seagrass beds 9 Coral Reef- Physiology # 5 East Coast rocky of coral growth and shore and/or sandy nutrition. shore (beach profiles), benthic faunaLaboratory write-up. Coral Reef Ecology 10 Deep-Sea Biology Oceanic Nekton 11 12 DVD/Blue PlanetCoral Reefs & Tutorial 5 Marine ecosystems & Tutorial 6 Management – Pollution and Degradation Marine ecosystems, Biodiversity and Human well being . Test 2 13 144 ADDITIONAL INFORMATION: Refer to the Life Sciences Undergraduate Handbook for general guidelines for teaching, assessment, student conduct, essential skills, and support at- http://sta.uwi.edu/fst/lifesciences/documents/handbook.pdf and to the General Information and General Regulations in the Faculty Booklet available fromhttp://sta.uwi.edu/resources/documents/facultybooklets/ScienceTechUndergrad.pdf Students are reminded that they must attend a minimum of 75% of the practical sessions and tutorials. Failure to do so will result in debarment from the final examination. As a general principle, medicals or other excuses may only excuse a student’s presence at an assigned time. Students must still complete the assigned work (make-up lab report or make-up test) in order to obtain the marks for that item of coursework. The student is responsible for liaising with the Course Coordinator or Teaching Assistants to ensure the assigned make-up is completed. Students are hereby informed that plagiarism is forbidden and all unsupervised coursework items must be accompanied by a Coursework Accountability Statement in order to be assessed. Specific items may require submission through Turnitin on myeLearning. Refer to ‘University Regulations on Plagiarism’ available from http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf HOW TO STUDY FOR THIS COURSE: Attendance is mandatory for lectures, tutorials and practicals. Prior preparation is strongly advised to able to fully participate in activities and obtain the full value of the sessions. Thorough use should be made of the resources provided and students are strongly advised to become familiar with them and start utilising them from the first week. Students are encouraged to interact regularly with staff even outside of the assigned tutorial times to ensure prompt, satisfactory solution of any problems. Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 145 40-44 F2 Weak 1.3 0-39 F3 Poor 0 J.F.GOBIN 05 MARCH 2013 THE UNIVERSITY OF THE WEST INDIES ST. AUGUSTINE FACULTY OF SCIENCE AND TECHNOLOGY DEPARTMENT OF LIFE SCIENCES Course code: BIOL 3162 Course Title: Principles of Microbial Biotechnology Number of Credits: 3 . Level: Undergraduate –Year III Pre-requisites: Biology Stream BIOL2265- Fundamentals of Microbiology BIOL 2164- Principles of Molecular Biology BIOL3369- Laboratory Skills in Biotechnology Biochemistry stream BIOC2262 Gene Expression BIOC3162- Experimental Biochemistry and Molecular Biology Anti-requisite: BIOL3262 Microbial Biotechnology Semester: 2 Offering Department: Department of Life Sciences, Faculty of Science and Technology, The University of the West Indies, St. Augustine Course description This course focuses on the applications of microorganisms in a range of processes that are beneficial for humans and the environment. The topics covered include isolation, screening, genetic manipulation and culturing of microorganisms for selected biotechnological applications related to industries, health, agriculture and the environment. The course is organized into face-to-face lectures, tutorials and practical exercises. General and specific concepts would be covered in lectures while tutorials would be interactive, with students expected to prepare and fully participate in discussions and other class activities. Students will be continuously assessed via in-course tests, activities during lectures and tutorials, and attendance and participation in tutorials. Students’ practical exercises will be assessed and there is also a final end-of-semester theory examination. 146 Purpose of course/rationale The course is a core requirement for the specialization in Biotechnology in the Department of Life Sciences, UWI, St. Augustine. It will be highly beneficial to students with core interests in microbiology and biotechnology. It is designed to introduce students to fundamental theoretical principles and develop key practical skills in microbial biotechnology. Microbial biotechnology is currently one of the most important sub-disciplines of biotechnology, having contributed significantly to medicine, agriculture, the environment and industries in terms of a host of new drugs, chemicals and processes. The prospects for microbial biotechnology are even brighter with the development of new metagenomic approaches of exploiting the potential of unculturable microorganisms, which represent the overwhelming majority of microbes in the environment. Instructor Information BIOL 3XXX will be managed by a course coordinator and would be taught by a team of experienced lecturers, supported by a Teaching Assistant/Instructor and laboratory demonstrators. Office hours for the coordinator and lecturers are posted on the faculty bulletin boards and are available from the Department of Life Sciences’ general office. Team members can also be contacted via e-mail. Name: Dr Adesh Ramsubhag (Co-ordinator) E-mail: [email protected] Office location: Rm. 220, Natural Sciences Building, Old Wing Office hours: Check Notice Boards Phone: 662-2002 Ext. 83086 Name: XXXX (Teaching assistant) E-mail: Office location: Office hours: Phone: 662-2002 Ext. Letter to students Welcome to BIOL 3XXX- Principles of Microbial Biotechnology. This course provides an exciting opportunity to be introduced into the exciting field of modern biotechnology. It would provide a platform for developing a fundamental understanding of theoretical concepts and practical skills in exploring and applying the enormous diversity of microbes for applications beneficial to humans and the environment. A major emphasis would be to develop critical thinking skills. Interactive participation in lectures, tutorials and practical sessions is essential. Please feel free to ask questions or offer alternative perspectives. You would also need to focus on personal attributes of team work and inter-personal skills required for group work, in addition to organizational skill and time management. I wish you the most interesting and intellectually stimulating experience in this course. Content: BIOL3XXX covers the following content: Overview of Microbial biotechnology: history, scope and future prospects Microbial bioprospecting: isolating and screening microorganisms for various applications Metagenomic approach for harnessing biotechnological potential of microorganisms Principles of fermentation Microbial production of proteins 147 Microbial production of primary metabolites: organic acids and amino acids Microbial production of secondary metabolites: antibiotics Recombinant and synthetic vaccines Microbial biofertilizers Biological control of pests and diseases Microbial production of polysaccharides and polyesters Ethanol production Bioremediation Improvement of microorganisms Course goal: The goal of the course is to expose students to general theoretical concepts and develop fundamental practical skills in microbial biotechnology. General Objectives: Students completing the course will have: 4. A critical review of biotechnological processes employing microbial cells or genes applied to medicine, the environment, agriculture and industries. 5. Fundamental practical skills in isolating, screening and applying microorganisms in biotechnology processes. Learning objectives: Upon completing this course, students will be able to - Devise culture based strategies for isolating and screening microorganisms for a range of biotechnological applications; - Review the metagenomic strategies for harnessing microbial genes for beneficial applications; - Compare and contrast culture based and metagenomic strategies for biotechnological exploitation of microorganisms; - Explain the culturing techniques used for obtaining microbial cells and metabolic products; - Explain the fundamental principles of microbial production of biological compounds, including the choice of organisms, culturing processes, metabolic pathways and means of improving productivity: o proteins o organic acids and amino acids o antibiotics o recombinant and synthetic vaccines o ethanol production o polysaccharides and polyesters - Formulate strategies for developing microbial biofertilizers and biopesticides; - Explain the principles of land farming, composting, biosparging and bioventing methods of bioremediation; - Compare and contrast in-situ and ex-situ bioremediation strategies; - Propose bioremediation strategies for in-situ and ex-situ treatment of petroleum contaminated soils; - Explain how microbes can be improved by conventional and genetic engineering techniques. Mode of Delivery: Lectures -18 hours: Tutorials -12 hours: Practical project- Didactic; interactive Interactive; mind maps; problem-solving Group project comprising of an equivalent to 12 hr/student 148 ASSIGNMENTS: Coursework for BIOL 3XXX will be assigned as follows: 6. Writing across curriculum exercises-5%. These activities are designed to encourage students to become active learners by engaging knowledge to further develop understanding. The exercises would help students understanding content and expressing ideas. A total of five simple exercises worth a total of 5 marks (1 mark per exercise) would be given during lectures or tutorials (no more than one per week). Each exercise would take between 5 – 10 minutes to complete and would comprise of varying activities including: a. Focused free-writing- Students may be asked to briefly write on their understanding or perspective on a subject/topic covered or on how the subject/topic may be connected to other situations. b. Entry slips- Short responses to questions posed at the beginning of class; c. Answering questions. One or few questions would be given which students must answer and submit for grading. d. Short summary. Students are asked to summarize main points of a reading assignment in a short paragraph. e. Group presentation- The tutorial class would be divided into groups and each group would be required to make a short presentation on a document provided. 7. In-course Test-20% One written in-course test would be given worth 10% per test in week 7. Two online tests will also be given in weeks 4 and 11 worth 5% each. The tests would comprise of MCQs and structured questions. 8. Practical reports-20% Students are required to conduct group projects aimed at isolating, screening, identifying and demonstrate potential usefulness of microorganism from the environment. The project is worth 20% as follows: proposal- 2%; literature review- 2%, poster presentation – 3%, oral presentation- 3%, final report – 6%, continuous assessment – 4%. 9. Class participation- 5% Student participation in tutorial sessions will be monitored by the tutor and a grade assigned at the end of the semester. Participation shall include more than just showing up at tutorial – some evidence of an active role in the tutorial discussion would be required to obtain the full 5% for participation. At the beginning of the semester, the students in each tutorial would engage in developing the exact assessment criteria for this assignment, and the student-developed criteria will be used to determine each student’s participation grade. Cheating, including plagiarism, would not be tolerated. Incidents of cheating would be dealt with according to UWI’s rules and regulations including: - Examination Regulations for First Degrees, Associate Degrees, Diplomas and Certificates. Section (B) Cheating. Regulations 96–102; http://sta.uwi.edu/resources/documents/Exam_and_GPA_regulations.pdf. - University Regulations on Plagiarism (First Degrees, Diplomas http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf. and Certificates). Assessment: 149 Course Work: 50% - Writing across the curriculum; critical thinking exercises - Incourse test o One written test o Two online tests - (2 X 5%) - Group Project - Tutorial Attendance and participation Final Semester Examination: 50% - One 2-hour comprehensive written paper comprising of o 5 short answer questions worth 6% each o 2 essay-type question worth 35% each - All questions in this paper shall be compulsory TOTAL 5% 10% 10% 20% 5% 30% 70% 100% EVALUATION: BIOL3XXX will be evaluated in two ways – (a) through the offices of the Class Representative and the Life Sciences Student-Staff Liaison Committee, and (b) an end of semester course evaluation survey. The class will elect four class representatives (one per lab stream), whose role is to act as a mediator between the Life Sciences academic staff and the students in the class. The representatives will attend Liaison Committee meetings (held at least twice per semester), where they will present feedback on the course to the Department for action. The UWI performs a course evaluation survey at the end of every semester, and this information will also be used for overall assessment of the course and guide possible actions for improvement in subsequent semesters. TEACHING STRATEGIES: A combination of teaching strategies will be adopted in BIOL3XXX. The primary teaching strategy will be based on the face to face classroom lectures and discussions. Videos of lecture presentations would be made available to students prior to the lecture session via podcast media. Students are advised to view these videos before the lecture so that more emphasis can be placed on discussions and answering questions, thereby facilitating deep learning. This would also afford the time to have assessment exercises on “writing across the curriculum”. MyeLearning will be utilized throughout the course as a means to provide access to course materials such as Powerpoint presentation files, animations, weblinks, lessons and quizzes. This medium would also be used as a portal for student-lecturer communications and the dissemination of coursework feedback. A total of 12 tutorial sessions would be given for the semester. These would be small group sessions which would be conducted by instructors or tutors, who would normally by senior postgraduate students in Life Sciences. Students must attend tutorial sessions. Tutorials sheets will be assigned prior to each session via myeLearning, and students are expected to attempt the solutions before coming to tutorial. The goal of the tutorial session is to give students a more hands-on experience with the course material and easier access to course instructors. Students will be expected to ask and answer questions on material that is unclear, propose solutions to questions on the tutorial sheet and to generally participate fully in the tutorial activities. Tutors will not merely be going through the answers to the tutorial questions in these sessions. The practical component of the course would comprise of a project to isolate, screen, identify and demonstrate potential usefulness of microorganism from the environment. Each group would be assigned to a demonstrator who would provide laboratory guidance in the completion of the project. 150 In order to pass the course, you must gain an overall passing mark of 40%. Any student who misses more than 25% of practical or tutorial sessions without a medical or other valid excuse can be debarred from writing the final exam. RESOURCES: Text books: Alexander N. Glazer and Hiroshi Nikaido, Microbial Biotechnology: Fundamentals of Applied Microbiology, Cambridge University Press; 2nd edition, October 1, 2007, ISBN-10: 0521842107 Suggested additional readings: - Lee Yuan Kun (Ed.), Microbial Biotechnology: Principles And Applications, World Scientific Pub Co Inc; 2 nd edition (August 24, 2006), ISBN-10: 9812566775 - Internet links to web-based resources will be provided to students Lab coat: - Each student should have suitable Lab coat to use for practical exercises. Students not having lab coats will not be permitted in the lab. COURSE CALENDAR/SCHEDULE: Course calendar: Week Lecture/Tutorial 1 Lecture 1: Introduction; Course overview; class rules, lecturer and student expectations, safety; introduction of project; Lecture 2: Overview of Microbial biotechnology: history, scope and future prospects Tutorial 1: Introduction and overview 2 Lecture 3: Microbial bioprospecting using culture-based strategies Tutorial 2: Microbial bioprospecting 3 Lecture 4: Metagenomic approach for harnessing biotechnological potential of microorganisms Lecture 5: Principles of fermentation Tutorial 3: Metagenomics 4 Lecture 6: Microbial production of proteins Tutorial 4: Fermentation and microbial production of proteins 151 Week Lecture/Tutorial Online test- MCQs (5%) 5 Lecture 7: Microbial production of primary metabolites: organic acids and amino acids Lecture 8: Microbial production of secondary metabolites: antibiotics Tutorial 5: Organic acids and amino acids 6 Lecture 9: Recombinant and synthetic vaccines Tutorial 6: Production of antibiotics and vaccines 7 Lecture 10: Microbial biofertilizers Tutorial 7: Biofertilizers 8 Written incourse test (in lecture slot) – MCQs, structured questions (10%) Lecture 11: Microbial biofertilizers Tutorial 8: Biofertilizers 9 Lecture 12: Biological control of pests and diseases Lecture 13: Biological control of pests and diseases Tutorial 9: Biological control 10 Lecture 14: Microbial production of polysaccharides and polyesters Lecture 15: Ethanol production Tutorial 10:Polysaccharides, polyesters and ethanol 11 Lecture 16: Bioremediation Lecture 17: Bioremediation Tutorial 11: Bioremediation Online test- MCQs (5%) 152 Week 12 Lecture/Tutorial Lecture 18: Improvement of microorganisms Tutorial 12: Bioremediation Project guidelines Week Activity Comments 1 Decide on project As guided by lecturer and TA 2 Write and submit preliminary project - No marks allocated. proposal- Must be written after consultation with - Title: demonstrator and lecturer - Introduction-some relevant background information and justification (problem, - Must be approved by lecturer before lab knowledge/technology gap; work can commence. relevance of research area in solving problem, bridging gap in knowledge/technology) - Goal and objectives: - Proposed approach (broad perspective of methodology without going into fine details) 3 Start laboratory work under guidance of Preliminary proposal much be approved before demonstrator starting 4 Final proposal due; Proposal worth 2 marks (to be marked by - Title: lecturer) - Introduction-some relevant background information and justification (problem, knowledge/technology gap; relevance of research area in solving problem, bridging gap in knowledge/technology) - Goal and objectives: - Proposed methodology – include all details) - Anticipated results - Expected outcomes (to science, humans etc) - Literature cited Continue laboratory work 153 5 Continue laboratory work 6 Literature review due Literature review worth 2 marks (To be marked by lecturer) Continue Laboratory work See: http://www.ais.up.ac.za/med/tnm800/tnmwritingliteraturereviewlie.ht m http://www.sdc.uwo.ca/writing/gradhandouts/Academic%20Tasks%20 -%20Literature%20Reviews%20-%20Presentation.pdf 7-10 Continue laboratory work Poster worth 3 marks (To be marked by a panel including lecturer, TA, other lecturers) Prepare poster presentation Work on final report 11 Oral presentation of research Oral presentation worth 3 marks (To be marked by a panel including lecturer, TA, other Make adjustments to final report if lecturers) necessary 12 Submit final report Final report worth 6 marks (To be marked by lecturer) Continuous assessment 4 marks (to be marked by demonstrators and TA based on observations and interviews with group members and laboratory technicians/assistants). Contributions to different phases would be separately assessed: - Conceptual framework and research proposal - Literature review - Isolations - Screening/evaluation of isolates - Identification - Storage of isolates - Preparation of poster - Final report The criteria for allocation of marks would include: - Participation - Punctuality - Laboratory etiquette - Quality of work 154 Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 155 THE UNIVERSITY OF THE WEST INDIES ST. AUGUSTNE FACULTY OF SCIENCE AND TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE CODE: COURSE TITLE: SEMESTER: LEVEL: NO OF CREDITS: BIOL 3164 Function and Design in Biology II II 3 PRE-REQUISITES: Either BIOL1262 Living Organisms 1 or BIOL1261 Diversity of Organisms or (BIOL1065 Diversity of Plants and Animals and AGRI1012 Microbiology); and either BIOL 2XXX Physiology of Plants or BIOL2761 Plant Physiology and either BIOL2XXX Physiology of Animals or BIOL2862 Animal Physiology; AND BIOL3XXX Plant Anatomy ANTI-REQUISITE(S): BIOL3264 Functional Design in Biology COURSE DESCRIPTION This course offers a fresh approach to the study of the structure and function of living things. It does not follow the traditional approach based on phylogeny, processes, or organ systems, but looks at how organisms are designed to best make use of the physical characteristics of the environment in which they live. The course goes further than presentation of didactic lectures. For example, students are asked to critique the commonly held belief that cells are the building blocks of living things, and instead consider that cells are incomplete subunits of the organism, so that morphology is not related to anatomy. In the same vein, the concept of Bernoulli’s Principle is shown to be inadequate to explain flight. Analogies are used wherever possible to explain concepts, such as comparing the anatomy of stems and bones to beams and girders, using the Forth Rail Bridge as an engineered analogue of stems. Lectures, tutorials, and practicals are designed to encourage thinking about concepts rather than remembering details. PURPOSE OF THE COURSE/COURSE RATIONALE The course provides an understanding of how organisms are constructed to optimize their various functions. Within the Life Sciences Department it will be an optional elective course. This 3 credit version of the course includes a reduction in theoretical background and elimination of derivation of equations in order to make the course approachable to students with a wider background. INSTRUCTOR INFORMATION Instructor: Dr. Gregor Barclay 156 Office phone: E-mail: Office location: Office hours: (868) 662-2002 x83112 [email protected] 324, Natural Sciences Building, Old Wing 8:30 - 4:30 daily Instructor: E-mail: Office phone: Office location: Office hours: Dr. Ayub Khan [email protected] (868) 662-2002 x83087 224, Old Wing, Natural Sciences Building 8:30 - 4:30 daily CONTENT The course begins with the rationale for Functional Design in Organisms and the historical background of biomechanics, leading into a class discussion of the similarly named but otherwise unrelated Intelligent Design. Course content includes both fundamental considerations of functional implications of organismal size, shape and geometry, composition, structure and strength, and mechanics of feeding and mobility. Scaling in biology Stress and strain in biology Biomechanics of cell walls, tissues and organs Locomotion on land Viscoelasticity of biological materials Flow in tubes Flight Swimming dynamics GOALS/AIMS The aim of this course is to enable students to understand how organisms overcome constraints imposed by the laws of physics and the properties of matter and capitalize on opportunities in ways that allow them to prosper. GENERAL OBJECTIVES The lectures and practical schedule are designed so that at the end of this course students should be able to: Differentiate between support systems Explain the mechanics of locomotion in the water and air, and on land. Contrast life dominated by viscous forces versus inertial forces Describe methods of locomotion and drag reduction Interpret experimental biomechanical evidence. LEARNING OUTCOMES 157 At the end of this course students will be able to: 1. Critique the cell theory versus the organismal concept of plant and animal construction. 2. Relate the surface area to volume of organisms in the context of size, shape, and geometry. 3. Evaluate the structural characteristics of various biological materials. 4. Compare the arrangement of support structures in organisms with beams, trusses, & columns. 5. Apply the physics of support to derive safety factors in support systems and tune design to the mechanical characteristics of the environment. 6. Categorize support systems: Tensile, strutted; nearly rigid surfaces, hollow cylinders and shells; pressurized systems, and muscular hydrostats. 7. Describe the mechanics of motility: Muscle and bone as levers; muscular hydrostats. 8. Evaluate the relationships between viscosity and flow displayed by shear stress, modulus, strain, and rate; laminar/turbulent flow, Reynolds numbers, streamlining, flow in biological pipes 9. Evaluate the influence of pressure on flow: Bernoulli’s Principle in nature- pressure and drag; skin friction; drag coefficient and flexibility; lift & circulation, dynamics of flight & swimming. COURSE ASSESSMENT Students are required to achieve a minimum grade of 40% overall, irrespective of their performance in individual components. In-course test 1: In-course test 2: Five tutorial exercises Five practical reports Final examination (2 hours) 10 % 10 % 10% 20 % 50 % TEACHING STRATEGIES Lectures, practicals, and tutorials are designed accommodate a range of learning styles, as outlined in Ch. 7 of the DLS undergraduate handbook. The course combines lectures/tutorials with interactive practical sessions that allow students to become familiar with performing and interpreting biomechanical experiments. Tutorials follow the practical sessions with students in small groups each with a dedicated demonstrator. 158 RESOURCES Essential Text S. Vogel, Comparative Biomechanics: Life's Physical World (2003), Princeton University Press Recommended texts Alexander, R. M (1999) Energy for Animal Life, Oxford University Press. Ennos, R. 2012. Solid Biomechanics. Princeton: Princeton University Press. Niklas, K.J. 1992. Plant Biomechanics. An Engineering Approach to Plant Form and Function.University of Chicago Press. Vogel, S. 1994. Life in Moving Fluids. Princeton University Press. Practical equipment: Stop watches, meter rulers, egg crushing apparatus, glassware, balances, spring scales, graph paper, etc. All equipment will be provided by the Department of Life Sciences. COURSE CALENDAR Week Lecture 1 Course Introduction and Overview of course requirements Basic Anatomy/Morphology Lab/Tutorial GFB GFB 2 Surface area/Volume: Size shape & geometry in biology GFB Scaling of size and function AK 3 Stress and strain in biology Shape, stress and architecture AK AK 4 Shape, stress and design of biological structures Plant cell wall biomechanics AK GFB 5 Cell wall and plant construction Evolution of trees GFB GFB 6 Review In course test 1 GFB GFB 7 Locomotion on land, ballistic movement Froude s u er a d gait AK AK Plant allometry Morphological design and strength Froude s u gait analysis er a d 159 Week Lecture 8 Viscoelastic behaviour of biological materials I Viscoelastic behaviour of biological materials II AK AK 9 Flow in tubes Bird flight GFB GFB 10 Drag reduction Swimming dynamics AK AK 11 Review In course test 2 AK AK 12 Course Review Course Review GFB AK Lab/Tutorial Tutorial on video on Physics of Plants Laminar and turbulent flow ADDITIONAL INFORMATION Refer to the Life Sciences Undergraduate Handbook for general guidelines for teaching, assessment, student conduct, essential skills, and support at http://sta.uwi.edu/fst/lifesciences/documents/handbook.pdf . Refer to the General Information and General Regulations in the Faculty Booklet available from http://sta.uwi.edu/resources/documents/facultybooklets/ScienceTechUndergrad.pdf . Students are reminded that they must attend a minimum of 75% of the practical sessions and tutorials. Failure to do so will result in debarment from the final examination. As a general principle, medicals or other excuses may only excuse a student’s presence at an assigned time. Students must still complete the assigned work (make-up lab report or make-up test) in order to obtain the marks for that item of coursework. The student is responsible for liaising with the Course Coordinator or Teaching Assistants to ensure the assigned make-up is completed. Students are hereby informed that plagiarism is forbidden and all unsupervised coursework items must be accompanied by a Coursework Accountability Statement in order to be assessed. Specific items may require submission through Turnitin on myeLearning. Refer to ‘University Regulations on Plagiarism’ available from http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf HOW TO STUDY FOR THIS COURSE: Attendance is mandatory for lectures, tutorials and practicals. Prior preparation is strongly advised to able to fully participate in activities and obtain the full value of the sessions. Thorough use should be made of the resources provided and students are strongly advised to become familiar with them and start utilising them from the first week. Regular updates on course progress and materials are also highly recommended and you should check into your UWI email and myeLearning on a frequent regular basis to review materials, assignments and activities. Students are encouraged to interact regularly with staff on their projects, even outside of the assigned tutorial times to ensure prompt, satisfactory solution of any problems and to monitor progress. 160 Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 161 THE UNIVERSITY OF THE WEST INDIES, ST. AUGUSTINE FACULTY OF SCIENCE & TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE OUTLINE COURSE CODE: BIOL3263 COURSE TITLE: INTRODUCTION TO BIOINFORMATICS NO. OF CREDITS: 3 LEVEL: III, Sem. 1 PREREQUISITE(S): BIOL2164 Principles of Molecular Biology COURSE DESCRIPTION: This course introduces students to bioinformatics tools and methods. It provides the conceptual background for using bioinformatics tools and application methods and offers skills and training on computational molecular biology and related fields. It gives an understanding about major advances in the analysis of genomes, sequences and their structures and also critically discusses the strength and limitations of the methods. The lecture component of this course provides the necessary conceptual backing and the practical component provides assignments for utilizing bioinformatics tools. Problem-based learning methods would be employed to teach the utility of bioinformatics tools. Teaching approaches include lectures, tutorials and lab sessions. Topics include (but not limited to) bioinformatics databases, sequence and structure alignment, protein structure prediction, protein folding, protein-protein interaction, simulation, and molecular dynamics. COURSE RATIONALE The global availability of genome sequence information for human, animal, plant and micro-organism systems has led to the development of new technologies dependent on bioinformatics. Scientists require the combined skills of molecular biology and computer science to exploit these data for the various applications. For those who wish to perform research in life sciences, it is essential to be aware of the databases and software programs. There is a current necessity to develop combined skills at the undergraduate level itself so that students have been well exposed to this emerging field. Basic knowledge on bioinformatics is essential for Biology students especially concentrating on biotechnology branch. Fundamental learning and training in this field would provide opportunities for undertaking advanced study or training in bioinformatics and computational biology wherein career opportunities are fast emerging at the present and future times. INSTRUCTOR INFORMATION 162 BIOL3XXX will be managed by a course coordinator and would be taught be a team of experienced lecturers, supported by a Teaching Assistant/Instructor and laboratory demonstrators. Office hours for the coordinator and lecturers are posted on the faculty bulletin boards and are available from the Department of Life Sciences’ general office. Team members can also be contacted via e-mail. Name of instructor(s): Office address and phone: Email address: Office hours: XXXXXX XXXXXX XXXXXX XXXXXX LETTER TO THE STUDENT Welcome to BIOL3XXX- Introduction to Bioinformatics. This course covers the basic bioinformatic concepts and methods widely used currently for bioinformatic analyses and research. Bioinformatic methods are increasingly used over multiple fields in molecular biology and biotechnology covering plants, microbe and by extension to applications of biotechnology including medicine, environment, industry and agriculture. The course is taught through a series of 24 lectures/tutorials and 6 lab (wet and virtual) sessions. The course is quite extensive and students must make a sincere effort to do well by fullest participation and involvement. It is important to attend lectures. Please make use of supplementary information provided to strengthen foundation and enhance understanding of material covered. SPECIAL REQUIREMENTS: Students should have basic mathematical skills which they would have acquired from the basic mathematics courses underwent in Year 1 or elsewhere. This course extensively uses computers and software. Therefore students are encouraged to own a computer system (laptop) and have in their possession throughout the course period. The required software is downloadable through privileged access and user sign up to all bonafide students registered for the course. CONTENT This course is designed to introduce the most important and basic concepts, methods, and tools used in Bioinformatics. Topics include (but not limited to) bioinformatics databases, sequence and structure alignment, protein structure prediction, protein folding, protein-protein interaction, simulation models, and molecular dynamics. Emphasis will be put on the understanding and utilization of these concepts and algorithms. The objective of this course is to introduce students in to field of bioinformatics and make them familiar with the use of basic bioinformatics tools. GOALS/AIMS This course aims to: Build a range of competencies in transferrable skills as applied to experimental bioinformatics and computational biotechnology. Expose students to the general techniques used for conducting research in Bioinformatics. Incorporate specialist practical skills and provides adequate pre-required hands on knowledge and exposure on bioinformatics techniques essential for undertaking higher studies/research/profession in bioinformatics and biotechnology. 163 LEARNING OUTCOMES At the end of the course students should be able to: Apply the major transferrable skills relevant to using tools in bioinformatics. Analyze the applicability of bioinformatics techniques in Biotechnology and Molecular biology. Explain, illustrate and interpret the principle, mechanism and experimental steps of various bioinformatics tools and analytical procedures. Review specific bioinformatics methodologies and tools used in research in selected problems in biotechnology. Demonstrate hands on lab skills in the analytical techniques covered. Apply bioinformatics techniques to the elucidation of real-life scenarios. Evaluate the applicability of bioinformatics techniques to experimental aims. Critique, analyze and discuss the relevant case studies/journal articles/projects and reports for relevance and applicability of methodology. COURSE ASSESSMENT 50% Course work 50% Final examination Course assessment components In-course tests (2x10%) Assignements/Online activities (3x5%) Tutorial participation & lab quizzes/lab project Final examination % split up details 20% 15% 15% 50% EVALUATION The elected Class Representative and/or Deputy will attend meetings with the course teacher(s) organized at every 4th week, and present feedback from the students attending that course or stream. This feedback is normally provided both orally and in written form for transmission to the lecturer. Apart from that the representatives will be attending the Liaison Committee meeting, and give their feedbacks to the committee. Students may comment on any aspect of the course or facilities. Students will be encouraged to submit their feedback (oral/written) during tutorials directly to the course teacher and appropriate actions will be taken by the teacher then and there. Results of in-course tests, quiz and other course assignments will be analyzed and presented in the class. This will help students to check their progress constantly and also helps the instructor to identify the weak areas and thereby could alert and advise students individually to alter their approach of study and completing the work. The final reflective feedback and comments about the entire course and teaching will be collected on the last day of the course. This will be saved for analysis and utilized as a base for improvement for the next offering in the following year. TEACHING STRATEGIES Credits: 3 computed as follows: Lectures and Tutorials: 24 h (2 cr) 164 Practical work: 24 h (1 cr) The instruction for this course would comprise of 2 lectures per week and one practical session per alternate week. Additionally, instructional videos, tutorials and virtual labs would be utilized to support the teaching. Formal lectures and tutorials will provide introduction to specific topics and allow for discussion in an interactive setting. Case studies, journal papers and data analyses will be carried out in small group settings. Course materials and readings will be available online in advance so that discussion and critiquing can take place within the assigned class time. This course is also mye-learning supported, and many other resources are readily available for the students. Students in the practical sessions, work in groups of 4 during these hands-on sessions, for which experimental planning (pre-labs) and small group discussions are required. RESOURCES Recommended reading: Mount, D. 2004. Bioinformatics: Sequence and Genome Analysis, II edn, Cold Spring Harbor Laboratory Press. Xiong, J. 2006. Essential Bioinformatics Cambridge University Press. Peyzner, P., Shamir, R. 2011. Bioinformatics for Biologists Cambridge University Press. Pevsner, J. 2009. Bioinformatics and Functional Genomics (2nd edition, Wiley-Blackwell. COURSE CALENDAR Week Lecture + Tutorial Wk1 Introduction to Bioinformatics Wk1 Importance and uses of bioinformatics Wk2 Information networks and uses Wk2 Nucleic Acid sequence Databases I Wk3 Nucleic Acid sequence Databases II Wk3 Tutorial Wk4 Wk4 Wk5 Protein sequence databases I Protein sequence databases II Metagenomics Wk5 Wk6 Wk6 Sequence Alignment tools I Sequence Alignment tools II Protein structure analyses I Wk7 Wk7 Wk8 Wk8 Wk9 Wk9 Wk10 Wk10 Wk11 Tutorial Protein structure analyses II RNA secondary structure Primer design Genome Projects Phylogenetic trees Tutorial Gene regulatory modules Micro RNA regulatory modules Lab DNA databases and sequence analysis Multiple Sequence Alignment In-course test; Sequence assembly Motifs and Patterns Gene structures, genotyping Incourse test; 165 Genome variations, primer design Wk11 Wk12 Wk12 Wk13 Wk13 Genome variations, Simple Polymorphisms Systematic Literature Search Microarray data analysis Review Tutorial Review Tutorial Nucleotide ADDITIONAL INFORMATION: Refer to the Life Sciences Undergraduate Handbook for general guidelines for teaching, assessment, student conduct, essential skills, and support at http://sta.uwi.edu/fsa/lifesciences/documents/handbook.pdf Refer to the General Information and General Regulations in the Faculty Booklet available from http://sta.uwi.edu/fsa/documents/UGFacultyBooklet.pdf. Students are reminded that they must attend a minimum of 75% of the practical sessions and tutorials. Failure to do so will result in debarment from the final examination. As a general principle, medicals or other excuses may only excuse a student’s presence at an assigned time. Students must still complete the assigned work (make-up lab report or make-up test) in order to obtain the marks for that item of coursework. The student is responsible for liaising with the Course Coordinator or Teaching Assistants to ensure the assigned make-up is completed. Students are hereby informed that plagiarism is forbidden and all unsupervised coursework items must be accompanied by a Coursework Accountability Statement in order to be assessed. Specific items may require submission through Turnitin on myeLearning. Refer to ‘University Regulations on Plagiarism’ at http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf HOW TO STUDY FOR THIS COURSE: Attendance is mandatory for lectures, tutorials and practicals. Prior preparation is strongly advised to able to fully participate in activities and obtain the full value of the sessions. Thorough use should be made of the resources provided and students are strongly advised to become familiar with them and start utilising them from the first week. Regular updates on course progress and materials are also highly recommended and you should be checking into myelearning on a frequent regular basis to review materials, assignments and activities. Students are encouraged to interact regularly with staff, even outside of the assigned tutorial times to ensure prompt, satisfactory solution of any problems and to monitor progress. Good group management is essential and requires good communication and interpersonal skills. Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 166 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 THE UNIVERSITY OF THE WEST INDIES, ST. AUGUSTINE FACULTY OF SCIENCE & TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE OUTLINE COURSE CODE: BIOL3363 COURSE TITLE: MEDICAL BIOTECHNOLOGY NO. OF CREDITS: 3 LEVEL: III, Sem. 1 PRE-REQUISITE(S): Principles of Molecular Biology COURSE DESCRIPTION Biotechnology as a field has very high relevance and application to human and animal medicine. With the advent of research we are at a stage to unravel the molecular mechanisms of several diseases and disorders. These studies have opened up a new era for the management of several problems facing human health and longevity. Biotechnology innovation is in a large part driven by the requirement for improvements in medical diagnosis and therapy for a range of diseases including autoimmune diseases, diseases of inflammation and cancer. This course gives students a detailed insight into the principles and techniques of biotechnology applied to human medicine. Topics include (but not limited to) biopharmaceuticals, stem cell technologies, tissue engineering and regenerative medicine, proteomics, antibody technologies, nanomedicine and molecular diagnostics. The teaching and learning methods include lectures/tutorials, and field trips to medical facilities (within Trinidad). COURSE RATIONALE This course focuses on some of the main areas in which biotechnology has generated medical products and areas of current research which promise to deliver “future cures”. The global availability of genome sequence 167 information for humans and various pathogenic organisms and information on molecular mechanisms have placed us in a right situation to develop and formulate precise treatments and diagnostic procedures for various diseases and disorders. For those who wish to perform research in health sciences, it is essential to be aware of the scientific developments taking place in this seminal field. There is a current necessity to develop good understanding of this field at the undergraduate level itself so that students have been well exposed to this emerging field. Biology students and those focusing on Biotechnology should be aware of advancements taking place in medical biotechnology. Fundamental learning and training on this field would provide opportunities for undertaking advanced study or research in medical biotechnology wherein career opportunities are emerging. INSTRUCTOR INFORMATION BIOL3XXX will be managed by a course coordinator and would be taught be a team of experienced lecturers, supported by a Teaching Assistant/Instructor and laboratory demonstrators. Office hours for the coordinator and lecturers are posted on the faculty bulletin boards and are available from the Department of Life Sciences’ general office. Team members can also be contacted via e-mail. Name of instructor(s): Office address and phone: Email address: Office hours: XXXXXX XXXXXX XXXXXX XXXXXX LETTER TO THE STUDENT Welcome to BIOL3XXX- Medical Biotechnology. We are living in the era of exciting medical technology which is dawning on the corridors of biotechnology. Fuelled by a greater understanding of how our bodies work and technological innovations, we are living longer and more productively than ever before. But still we are struggling to find cures and remedies for several life threatening diseases and conditions, for which biotechnology should store the solution. This course investigates these advances; it provides an overview of medical conditions with their implications and the technologies developed to combat them. Subjects covered include biopharmaceuticals, stem cell technologies, tissue engineering and regenerative medicine, proteomics, antibody technologies, nanomedicine and molecular diagnostics. The course is taught through a series of lectures/tutorials, and field trips. The course is quite extensive and students must make a sincere effort to do well by fullest participation and involvement. It is important to attend lectures. Please make use of supplementary information provided to strengthen foundation and enhance understanding of material covered. CONTENT This course is designed to introduce the most important applications of biotechnological advancements for human medicine and therapy. The course includes study of molecular mechanisms underlying important diseases, applications of biotechnology to the diagnosis and treatment of disease as well as the development of drugs and therapeutic agents. The topics include by not limited to: molecular mechanisms of diseases, molecular diagnostic procedures, drug development, novel therapeutic technologies including antibody therapy, stem cell therapy, somatic gene transfer and gene therapy, novel vaccines, nanomedicines, tissue engineering & regenerative medicine and novel bio-materials for human medicine. The objective is to help the students to expose to the frontiers of biotechnological research developments and to reinforce the importance of this field to enable future advancements. GOALS/AIMS 168 This course aims to: Present students with the background to medical problems and the technologies currently used to address them through biotechnological means. Expose to scientific development and application of these new technologies as well as their limitations. Present opportunities for further investigation and tests their innovative thought processes. Expose and update students to the constantly evolving biotechnological procedures and techniques and motivate them to develop interest and for undertaking higher studies/research in medical biotechnology. LEARNING OUTCOMES At the end of the course students should be able to: Discuss the concepts, principles, key techniques and milestone achievements that have engendered the rise of Medical Biotechnology as means to find solutions to current health care challenges. Analyse, interpret and critically evaluate the emerging technologies. Justify the appropriateness of selected technologies in developing novel approaches for human health and cure. Evaluate the ethical components of novel technologies and appreciate their relevance in the spirit of sustainable human life and health. Analyse primary research papers, evaluate complex information and creatively use this information in the synthesis of their own presentations. Extrapolate and apply the learned new technologies for finding solutions for local, regional and global health problems and real-life scenarios. Critique, analyze and discuss the relevant case studies, projects and reports for relevance and applicability of methodology. COURSE ASSESSMENT* 50% Course work 50% Final exam Course assessment components In-course tests (2x10%) Assignment & Presentations (10%) and Journal club (10%) Tutorial participation (3%) & field trip reports (7%) Final examination % split up details 20% 20% 10% 50% EVALUATION The elected Class Representative and/or Deputy will attend meetings with the course teacher(s) organized at every 4th week, and present feedback from the students attending that course or stream. This feedback is normally provided both orally and in written form for transmission to the lecturer. Apart from that the representatives will be attending the Liaison Committee meeting, and give their feedbacks to the committee. Students may comment on any aspect of the course or facilities. Students will be encouraged to submit their feedback (oral/written) during tutorials directly to the course teacher and appropriate actions will be taken by the teacher then and there. 169 Results of in-course tests, quiz and other course assignments will be analyzed and presented in the class. This will help students to check their progress constantly and also helps the instructor to identify the weak areas and thereby could alert and advise students individually to alter their approach of study and completing the work. The final reflective feedback and comments about the entire course and teaching will be collected on the last day of the course. This will be saved for analysis and utilized as a base for improvement for the next offering in the following year. TEACHING STRATEGIES* Credits: 3 computed as follows Lectures+Tutorials: 26 h (2.2 cr) Practicals: 20 h (0.8 cr) The instruction for this course would comprise 2 Lectures with inbuilt tutorials (40 min lecture+10 min tutorial) per week and 4 practicals involving visits to medical and research facilities. Additionally, instructional videos would be utilized to support the teaching. Formal lectures and tutorials will provide introduction to specific topics and allow for discussion in an interactive setting. Case studies/journal papers discussion will be carried out in small group settings. Course materials and readings will be available online in advance so that discussion and critiquing can take place within the assigned class time. This course is also myeLearning supported, and many other resources are readily available for the students. RESOURCES Recommended reading: Nallari, P., Veugopal Rao, V. 2010. Medical Biotechnology, 1/e, Oxford Univ. Press, India. Pongracz, J., Mary Keen, M. 2008. Medical Biotechnology, Elsevier Health Sciences. Killeen, A. 2004. Principles of Molecular Pathology, Humana Press. 170 COURSE CALENDAR* Week Wk1 Wk1 Wk2 Wk2 Wk3 Wk3 Wk4 Wk4 Wk5 Wk5 Lecture + Tutorial Lab Introduction to Biotechnology and Human health Molecular Mechanisms of Diseases Molecular Mechanisms of Diseases Importance of human genomics and proteomics in health and cure (Journal 1) Principle of Therapeutics & Drug Action Molecular Medicine Molecular Medicine Biochemical Correlates of Diseases (Journal 2) Metabolomics and human medicine Clinical Immunoassays Techniques Wk6 Molecular diagnostics Wk6 Wk7 Wk7 Wk8 Wk8 Wk9 Molecular diagnostics Role of Vaccines in Medicine Novel vaccines Recombinant Therapeutic Proteins Antibody Therapies (Journal 3) Wound Healing and Biomaterials Wk9 Biopharmaceutical products (Assignment) Wk10 Wk10 Drug Discovery Assays (Journal 4) Nanodrugs Wk11 Wk11 Stem cell therapies Gene therapies Wk12 Therapeutic Drug Monitoring (Journal 5) Wk12 Personalized medicine and Ethical components of biotechnology Review Tutorial Review Tutorial Wk13 Wk13 Visit to Mount Hope diagnostic facility In-course test Visit to Cancer hospital Presentation; Visit to cell culture facility Visit to pharmacology facility Visit to Virology lab, In-course test 171 ADDITIONAL INFORMATION: Refer to the Life Sciences Undergraduate Handbook for general guidelines for teaching, assessment, student conduct, essential skills, and support at http://sta.uwi.edu/fsa/lifesciences/documents/handbook.pdf. Refer to the General Information and General Regulations in the Faculty Booklet available from http://sta.uwi.edu/fsa/documents/UGFacultyBooklet.pdf . Students are reminded that they must attend a minimum of 75% of the practical sessions and tutorials. Failure to do so will result in debarment from the final examination. As a general principle, medicals or other excuses may only excuse a student’s presence at an assigned time. Students must still complete the assigned work (make-up lab report or make-up test) in order to obtain the marks for that item of coursework. The student is responsible for liaising with the Course Coordinator or Teaching Assistants to ensure the assigned make-up is completed. Students are hereby informed that plagiarism is forbidden and all unsupervised coursework items must be accompanied by a Coursework Accountability Statement in order to be assessed. Specific items may require submission through Turnitin on myeLearning. Refer to ‘University Regulations on Plagiarism’ at http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf HOW TO STUDY FOR THIS COURSE: Attendance is mandatory for lectures, tutorials and practicals. Prior preparation is strongly advised to able to fully participate in activities and obtain the full value of the sessions. Thorough use should be made of the resources provided and students are strongly advised to become familiar with them and start utilising them from the first week. Regular updates on course progress and materials are also highly recommended and you should be checking into myelearning on a frequent regular basis to review materials, assignments and activities. Students are encouraged to interact regularly with staff, even outside of the assigned tutorial times to ensure prompt, satisfactory solution of any problems and to monitor progress. Good group management is essential and requires good communication and interpersonal skills. Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 172 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 THE UNIVERSITY OF THE WEST INDIES, ST. AUGUSTINE FACULTY OF SCIENCE & TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE DOCUMENTATION COURSE CODE: BIOL3366 COURSE TITLE: PLANT BIOTECHNOLOGY AND GENETIC ENGINEERING NO. OF CREDITS: 3 LEVEL: III, Sem. 2 PRE-REQUISITE(S): Either BIOL2XXX Principles of Molecular Biology and BIOL3XXX Laboratory Skills in Biotechnology OR BIOL3XXX Gene Expression and BIOL3XXX Experimental Biochemistry and Molecular Biology. ANTI-REQUISITE: BIOL3762 Plant Biotechnology COURSE DESCRIPTION This course introduces students to plant transformation technologies and genetic engineering methodologies for the introduction of beneficial traits into economically important plants. It also introduces students to plant tissue culture techniques and the impact of this technology on preservation of plant species and plant tissue based production of proteins and secondary metabolites. Topics include, Tissue culture applications in plant biotechnology; Advanced study of Gene sources and Gene expression; Promoters, selectable markers and reporter genes; Plant Transformation systems; Biology of Agrobacterium - mediated transformation; Agrobacterium – mediated gene transformation – methodology; Direct gene-transfer methods, Particle bombardment; Transgene Integration; Evaluation of Transgenics; Management of Gene silencing; Genetic engineering of plants for novel traits; herbicide tolerance, enhancing pest resistance, disease resistance; resistance to plant viruses, enhanced product qualities; Marker aided selection and gene pyramiding; Biofarming and plant expression systems; Phytoremediation, Genetic engineering of biofuel crops; Genetically modified crops - ethical, social biosafety and environmental issues. The teaching and learning methods include lectures/tutorials, group discussion, journal paper discussion and lab sessions. The teaching and learning methods include lectures/tutorials, and lab sessions. 173 COURSE RATIONALE This course focuses on advancements in plant biotechnology, which have provided solutions to present day agricultural problems. In the light of gradual paucity of natural resources and environmental concerns, agricultural productivity which has attained a plateau could be further improved only through biotechnological approaches. Otherwise situations may jeopardize global food production and would lead to food shortage in the near future. The global availability of genome sequence information and gene cloning of important plants have placed us in a right situation to develop biotechnology/genetic engineering-based solutions to the current problems of crop and food production. For those who wish to perform research in life sciences, it is essential to be aware of the scientific developments taking place in this seminal field. There is a current necessity to develop good understanding of this field at the undergraduate level itself, so that students have been well exposed to this emerging field. Fundamental learning and training on this field would provide opportunities for undertaking advanced study plant sciences and biotechnology. INSTRUCTOR INFORMATION BIOL3XXX will be managed by a course coordinator and would be taught be a team of experienced lecturers, supported by a Teaching Assistant/Instructor and laboratory demonstrators. Office hours for the coordinator and lecturers are posted on the faculty bulletin boards and are available from the Department of Life Sciences’ general office. Team members can also be contacted via e-mail. Name of instructor(s): Office address and phone: Email address: Office hours: Dr. Jayaraj Jayaraman Rm.220, Nat Sci. Bldg [email protected] Consult Life Sciences office LETTER TO THE STUDENT Welcome to Plant biotechnology and genetic engineering. The global population is predicted to peak at over 9 billion during the second half of this century. To provide food and dignified living conditions, agricultural productivity should have to increase 50% by 2030 and 70% by 2050. This task is extremely urgent and challenging, as it will have to coincide with a transition to more sustainable agricultural practices and as agricultural land is lost due to climate change and urbanisation. This course will focus on plants in exploring the fundamental constraints and analyze biotechnological opportunities and solutions for sustainable food production, as well as the use of plants to supply high-value products. Topics to be covered include, application of plant tissue culture applications in plant biotechnology and genetic engineering, plant transformation systems; management of gene silencing; genetic engineering of plants for novel traits; herbicide tolerance, enhancing diseases and pest resistance, biofarming, enhanced product qualities; Marker aided selection and gene pyramiding; genetically modified crops - ethical, social biosafety and environmental issues. The course is taught through a series of 26 lectures and 6 lab (wet and virtual) sessions. The course is quite extensive and students must make a sincere effort to do well by fullest participation and involvement. It is important to attend lectures and labs. Please make use of supplementary information provided to strengthen foundation and enhance understanding of material covered. CONTENT 174 This course explores the broad area of plant biotechnology and plant genetic engineering, including the scientific principles underpinning these practices. Topics covered are: Introduction to Plant Biotechnology; Principles of Plant tissue culture techniques; Tissue culture applications in plant biotechnology and genetic engineering; Advanced study of Gene sources and Gene expression; Promoters, selectable markers and reporter genes; Plant Transformation systems; Biology of Agrobacterium - mediated transformation; Agrobacterium – mediated gene transformation – methodology; Direct gene-transfer methods, Particle bombardment; Transgene Integration; Evaluation of Transgenics; Management of Gene silencing; Genetic engineering of plants for novel traits; herbicide tolerance, enhancing pest resistance, disease resistance; resistance to plant viruses, enhanced product qualities; Marker aided selection and gene pyramiding; Biofarming and plant expression systems; Phytoremediation, Genetic engineering of biofuel crops; Genetically modified crops - ethical, social biosafety and environmental issues. The lab sessions covered under BIOL3XXX- Laboratory Skills in Biotechnology will complement and partially fulfil practical components related to this course. GOALS/AIMS This course aims to: Present students with the background to the problems faced in agriculture and food production and the technologies conceived through biotechnological means to address them. Expose to the scientific development and application of plant biotechnology and genetic engineering methods and understand their efficiencies and limitations. Expose and update students to the constantly evolving biotechnological procedures and techniques and motivate them to develop interest and for undertaking higher studies/research in plant biotechnology. LEARNING OUTCOMES At the end of the course students should be able to: Discuss the concepts, principles, key techniques and milestone achievements that have engendered the rise of plant biotechnology as solution to current food crisis and uncertainties in agricultural production. Analyse, interpret and critically evaluate the emerging technologies. Justify the importance of novel biotechnological approaches for improving food production in the near future. Appraise the environmental sustainability of genetically modified crops. Evaluate the biosafety of genetically modified crops. Explore fullest clarification for the commonest disbeliefs revolving around genetically modified crops. Analyse primary research papers, evaluate developed technologies and creatively use this information in the synthesis of their own presentations. Extrapolate and apply the learned new technologies for finding solutions for local, regional and global agricultural problems and real-life scenarios. Critique, analyze and discuss the relevant case studies, projects and jornal papers for relevance and applicability of methodology. COURSE ASSESSMENT 50% Course work 50% Final exam Course assessment components % split up details 175 In-course tests (2x10%) 20% Assignments/Presentations (9%) and 20% Journal club (8%) and Tutorial participation (3%) Lab Quizzes (2x3=6%) & Lab observation 10% reports (4%) Final examination 50% EVALUATION The elected Class Representative and/or Deputy will attend meetings with the course teacher(s) organized at every 4th week, and present feedback from the students attending that course or stream. This feedback is normally provided both orally and in written form for transmission to the lecturer. Apart from that the representatives will be attending the Liaison Committee meeting, and give their feedbacks to the committee. Students may comment on any aspect of the course or facilities. Students will be encouraged to submit their feedback (oral/written) during tutorials directly to the course teacher and appropriate actions will be taken by the teacher then and there. Results of in-course tests, quiz and other course assignments will be analyzed and presented in the class. This will help students to check their progress constantly and also helps the instructor to identify the weak areas and thereby could alert and advise students individually to alter their approach of study and completing the work. The final reflective feedback and comments about the entire course and teaching will be collected on the last day of the course. This will be saved for analysis and utilized as a base for improvement for the next offering in the following year. TEACHING STRATEGIES Credits: 3 computed as follows: Lectures+Tutorials: 24 h (2 cr) Lab sessions: 24 h (1 cr) The instruction for this course would comprise of 2 Lectures with inbuilt tutorials per week and 5 lab sessions+1 lab presentation. Additionally, instructional would be utilized to support the teaching. Formal lectures and tutorials will provide introduction to specific topics and allow for discussion in an interactive setting. Case studies, journal papers and data analyses will be adopted in small group settings. Course materials and readings will be available online in advance so that discussion and critiquing can take place within the assigned class time. This course is also myeLearning supported, and many other resources are readily available for the students. RESOURCES Recommended reading: 176 Slater, A., Scott, N.W., Fowler, M.R. 2008. Plant Biotechnology: The Genetic Manipulation of Plants, Oxford, 2008. Oksman-Caldentey, K. Barz, H.B. (Ed). 2002. Plant Biotechnology and Transgenic Plants, CRC Press. Primrose, S.B., Twyman, R.M., Old, R.W. 2001. Principles of gene manipulation. Wiley-Blackwell. Dodds, J.H. 2004. Plant Genetic Engineering, Cambridge University Press. Razdan, M.K. 2003. Introduction To Plant Tissue Culture, 2/E, Oxford & IBH Publishing Company Pvt. Ltd. Journals: Plant Biotechnology journal, Journal of Plant Biochemistry and Biotechnology, Transgenic research Journal articles and reviews - provided by the lecturer COURSE CALENDAR Week Wk1 Wk1 Wk2 Wk2 Wk3 Wk3 Wk4 Wk4 Wk5 Wk5 Wk6 Wk6 Wk7 Wk7 Wk8 Wk8 Wk9 Wk9 Wk10 Wk10 Wk11 Wk11 Wk12 Lecture + Tutorial Introduction to the course requirements Introduction to Plant tissue culture Applications of plant tissue culture in biotechnology Plant Transformation systems Promoters, selectable markers and reporter genes (Journal 1) Biology of Agrobacterium - mediated transformation Agrobacterium – mediated gene transformation – Methodology Particle bombardment & direct gene-transfer methods Transgene Integration and Evaluation of Transgenics (Journal 2) Management of Gene silencing Genetic engineering for herbicide tolerance Genetic engineering for pest resistance Genetic engineering for disease resistance (Journal 3) Lab Agro-transformation PIG-based transformation Incourse test, Protoplast transformation Genetic engineering for resistance to plant viruses Genetic engineering for enhanced product qualities Genetic engineering for tolerance to abiotic stresses Genetic engineering for improved photosynthetic Transient gene expression efficiency and novel traits (Journal 4) assays (Quiz 1) Biotechnology for biofuels Metabolic engineering Biofarming Plant-made pharmaceuticals (Journal 5) Transgene - analysis through PCR (Quiz 2) Genetically modified crops: ethical, social and environmental issues Genetically modified crops: prospects and constraints – up-to-date (Assignment) Marker aided selection 177 Week Wk12 Lecture + Tutorial Marker aided selection & gene pyramiding Wk13 Wk13 Review Tutorial Review Tutorial Lab Incourse test & Presentations ADDITIONAL INFORMATION: Refer to the Life Sciences Undergraduate Handbook for general guidelines for teaching, assessment, student conduct, essential skills, and support at http://sta.uwi.edu/fsa/lifesciences/documents/handbook.pdf . Refer to the General Information and General Regulations in the Faculty Booklet available from http://sta.uwi.edu/fsa/documents/UGFacultyBooklet.pdf. Students are reminded that they must attend a minimum of 75% of the practical sessions and tutorials. Failure to do so will result in debarment from the final examination. As a general principle, medicals or other excuses may only excuse a student’s presence at an assigned time. Students must still complete the assigned work (make-up lab report or make-up test) in order to obtain the marks for that item of coursework. The student is responsible for liaising with the Course Coordinator or Teaching Assistants to ensure the assigned make-up is completed. Students are hereby informed that plagiarism is forbidden and all unsupervised coursework items must be accompanied by a Coursework Accountability Statement in order to be assessed. Specific items may require submission through Turnitin on myeLearning. Refer to ‘University Regulations on Plagiarism’ at http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf HOW TO STUDY FOR THIS COURSE: Attendance is mandatory for lectures, tutorials and practicals. Prior preparation is strongly advised to able to fully participate in activities and obtain the full value of the sessions. Thorough use should be made of the resources provided and students are strongly advised to become familiar with them and start utilising them from the first week. Regular updates on course progress and materials are also highly recommended and you should be checking into myelearning on a frequent regular basis to review materials, assignments and activities. Students are encouraged to interact regularly with staff, even outside of the assigned tutorial times to ensure prompt, satisfactory solution of any problems and to monitor progress. Good group management is essential and requires good communication and interpersonal skills. Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 178 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 THE UNIVERSITY OF THE WEST INDIES, ST. AUGUSTINE FACULTY OF SCIENCE & TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE DOCUMENTATION 2015/16 COURSE CODE and TITLE BIOL3409 Caribbean Coral Reefs NO. OF CREDITS 3 LEVEL* 3 SEMESTER 2 PREREQUISITES BIOL3063 Marine Ecology and Oceanography LETTER TO THE STUDENT Dear students, Welcome to BIOL3409 Caribbean Coral Reefs. This advanced-level course on the coral reefs of the Caribbean places emphasis on the major ecological interactions within coral ecosystems, especially those of the Caribbean region. This course offers a mix of theoretical knowledge and practical skills development, which is valuable for those aiming for careers in marine biology, conservation, environmental consulting and marine management. The course will be taught through lecture sessions with the lecturers as well as special invited lecturers for certain topics. There will be individual and group assignments, the latter of which should help you to develop 179 communication and team skills that are important in the world of work. It is in your best interest to ask questions and ensure that you understand the concepts and techniques covered in this course. This course will be taught in blended mode comprising face-to-face lectures, tutorials and practical exercises, as well as online delivery of resource materials and diverse activities. You should engage with the course materials and participate fully to develop the essential skills of critical thinking (clarity, accuracy, relevance, logic, breadth, depth, precision, significance, completeness and fairness). Activities will be diverse, and you are strongly encouraged to ask questions, offer new ideas, problem-solve, and think innovatively to maximise the value of your learning experience. There will also be individual and group assignments, which should help you to develop communication and team skills that are important in the world of work. If sea conditions allow, there will be one field trip to visit a coral reef. The trip will be made if there are lowenough tides to allow safe access to the reef, and fairly clear water. At such times reef is too shallow for swimming so you will be able to explore it by walking in the shallow channels between the coral heads. You do not need to know how to swim, but you should be comfortable enough with water to be able to explore the reef using a snorkel and mask. To do that, you will need to put your face in the water. Please read the course outline carefully and keep the course schedule close to hand as it contains all the activities and assessments for the semester. In particular, take note of the policy on plagiarism and attendance requirements. We are here to ensure your success in this course and at UWI generally, and encourage you to come to us with any academic or other challenges you may face that could affect your attendance and performance. We look forward to meeting each of you and engaging in productive stimulating discussions over the coming semester. Dawn A. T. Phillip Course coordinator INSTRUCTOR INFORMATION This course will be taught by a team of lecturers, teaching assistants and demonstrators. Further logistical support will be provided by laboratory and field support staff. Office hours are posted on departmental notice boards and the main Life Sciences office A.T. 2nd floor, new wing, Natural Sciences building, ext. 82047 and 82208 [email protected] (preferred method of contact) Course coordinator Lecturer Dr. Dawn Phillip Lecturer Dr Stemann Instructor Mr Jahson Alemu I Peter Bacon Postgraduate Laboratory, 2nd floor, Natural Sciences building, ext. 33084 [email protected] (preferred method of contact) Teaching assistant Mr Guy Marley Teaching assistants’ office, 2nd floor, new wing, Natural Sciences building, ext. 82204 [email protected] (preferred method of contact) Thomas [email protected] (preferred method of contact) 180 WEEKLY SCHEDULE Lectures and tutorials Mondays noon–012:50 pm C1 (You are required to attend all Wednesdays 8:00–8:50 am C2 sessions) Practical classes Tuesdays2 2:00–06:00 pm LS3 (Weeks 3, 5, 9 and 11 – attend all sessions) COURSE DESCRIPTION This course develops students’ knowledge and understanding of the biology of reef-building corals, the diversity and ecology of coral reef communities, the importance of coral reefs, and the contemporary and historical challenges they face. In addition, students will be introduced to the ecosystem-based approach to reef management, and to the economic valuation of reefs. Though the course focusses primarily on Caribbean coral reefs, reference will be made to reefs in other parts of the world. COURSE RATIONALE Caribbean coral reefs are the foundation the Caribbean’s tourism and fishing industries, and thus account for a sixth of the region’s jobs, and a third of its income (or US$15 billion year-1), and 500 000 tonnes of its food. Caribbean reefs, however, are suffering so terribly from the effects of overexploitation, pollution and climate change that it is predicted that all the region’s corals may be dead within 40 years. Sustainably managing the region’s reef resources has proved difficult because most Caribbean nationals are ignorant of the devastation to their reefs, and most reef conservation efforts are either understaffed or highly dependent on costly imported personnel. This course aims to increase the number of graduates with applicable knowledge of coral biology, coral reef geology, ecology and conservation methods, thereby helping to provide the region with the skilled workforce it needs to achieve the long-term conservation of its reef resources, to conduct vital new research, and to sustain economically-important, reef-dependent industries, like tourism and fisheries. COURSE CONTENT An introduction to the reef geography of the wider Caribbean and history of reef resource use in the Caribbean. Coral biology, including taxonomy, anatomy and skeletal morphology, endosymbiosis with zooxanthellae, calcification and growth, nutrition, defensive behaviour, reproduction and recruitment. Environmental conditions required for coral reef formation, geological history of Caribbean reef formation and types of reefs; dynamics of reef structure formation and erosion. Reef community structure, zonation and dynamics. Major reef-associated organisms with attention to their ecological function. Uses, including reef fisheries, tourism and recreation, biodiversity and marine products, and ecosystem services. Valuation, including total economic value, use values, option values and non-use values. The threats and future challenges to Caribbean coral reefs, including natural disturbances and anthropogenic activities; hurricanes, tsunamis, and earthquakes; coral diseases and diseases of other reef organisms; overfishing, deterioration of water quality, physical destruction of reefs, climate change, invasive species. An introduction to monitoring methods and the ecosystem-based approach to reef management, including examples of mitigation actions appropriate to different geographic scales. 2 There is tentatively a field trip on Saturday 19th March 181 GOALS AND AIMS This course aims to Develop students’ competence in basic methods and approaches for identifying reef species and evaluating coral reef ecosystems Develop students’ awareness that resource users of coral reef ecosystems are complex and include social beings operating in various socio-cultural and socio-political contexts that must be understood by managers in order to effectively manage the resource base Introduce students to the range of options that have been used to manage coral reefs and reef resources GENERAL OBJECTIVES The general objectives of this course are to provide course materials, activities and assessments using both face-to-face and online modalities facilitate opportunities for students to engage with general concepts and specific examples to develop a broad understanding of the complex nature of human impacts on their environment guide students in crafting questions and critically analysing and exploring answers to these questions using investigative hands-on approaches facilitate discussion on the relationship between humans and their environment and evaluate potential solutions for ensuring sustainability and wellbeing of human populations LEARNING OUTCOMES Upon successful completion of the course, students should be able to: Identify some of the most important Caribbean coral species and describe their biology. Categorise the major ecological interactions within coral reef communities. Evaluate natural and anthropogenic threats to Caribbean coral reefs and select appropriate mitigation measures. Conduct a monitoring exercise to assess reef health and interpret the data generated. Construct a reef management plan using the ecosystem-based approach to management. Demonstrate basic skills for researching, analysing, synthesising, evaluating and communicating published and original environmental information; Evaluate specific human-environment interactions and identify resulting environmental issues; Develop solutions using a variety of tools and strategic approaches; manage group interactions and work effectively in teams. Students should also gain practical skills that will allow them to Describe a local coral reef ecosystem and the variety of species Accurately identify commercially important reef species using species identification keys Sample reef systems to collect community and habitat data COURSE ASSESSMENT The final mark for this course comprises the following Theory examination: one 2-hour written examination Course work 50% 50% Consisting of: – Four laboratory and/or field reports (practical) (10%) 182 – One research report (practical) (30%) – Short, weekly quizzes and in-class activities (theory) (10%) ASSIGNMENTS In-course assignments comprise 1. Practical reports, collectively worth 10% of the final course mark. The reports will be based on activities engaged in during four scheduled laboratory sessions as stipulated in the course schedule. Each report will utilise a different format: scientific drawings, traditional laboratory report, poster, PowerPoint presentation, video. 2. A single research project report worth 30% of the final course mark. This research project will be based on observations made, and data collected during the field trip, but analysed in the consecutive practical session. 3. A single literature-based essay worth 10% of the final course mark. The essay will be a reading-based assignment focussed on original literature and technical reports. EVALUATION Feedback on the course will be obtained informally from students on an on-going basis by regular interactions and meetings among students, demonstrators, teaching assistants and the course coordinator in lectures, tutorials and laboratory sessions. Formal feedback will be via election of class representatives who sit on the departmental Student-Staff Liaison Committee meetings held twice during the semester. Class representatives will channel both o er s a d o e datio s to the eeti g as guided the depart e t s sta dard operati g pro edures. Formal evaluation of the entire course will be accomplished via a UWI course evaluation questionnaire administered anonymously and confidentially at the end of the semester. All feedback will be considered on an on-going basis and corrective actions or adjustments made or discussed with students promptly or incorporated the following year. TEACHING STRATEGIES This course will be taught through a mixture of lectures, practical exercises and tutorials. There may also be guest lectures by experts. Lectures and tutorials in the first half of the semester will provide the conceptual and factual basis for specific topics and allow for discussion in an interactive setting. Case studies and selected topics may be presented by guest lecturers. Course materials and readings will be available online in advance to allow students to be better prepared to engage the lecturers in discussion during class time. Laboratory sessions will provide hands-on training at a coral reef. Lecturers and demonstrators will be present to provide guidance In addition, the online teaching tool, myeLearning, will be used extensively during this course for communication among students and staff (email, discussions) official posting of important notices (coursework assessment notices, instructions, and in-course results) provision of course details, lecture notes, practical guides, tutorial briefings provision of recommended resource materials and links to resources on specific websites discussion on selected topics Face-to-face contact time will be 36 credit hours, comprising: 183 Lectures 24 hours Tutorials 2 hours Practical exercises 20 hours Lectures will provide valuable synthesis and evaluation of the coral reef biology, values, threats and conservation measures related to these ecosystems. Lectures will also be used to update current issues and events, and prioritise content relevant to course assessment. Case studies and selected topics may be presented by guest lecturers. Lecture sessions will be interactive, with students encouraged to ask questions, share knowledge and experiences. Course materials and readings will be available online in advance to allow students to be better prepared to engage the lecturers in discussion during class time. Tutorials will cover course topics in an interactive format using a variety of collaborative active learning techniques. In addition to clarifying details of course content, specific transferrable skills may be addressed, e.g., essay and report writing, critiquing and oral presentation. Four 5-hr practical sessions3 will provide hands-on experience for students to gain skills required for conducting well-designed coral reef monitoring and research. Some time will also be assigned during these sessions for demonstrators and lecturers to guide students on their research projects. RESOURCES Most resources are available myeLearning including Lecture outlines – including learning objectives, summaries, recommended readings, some presentations Resources – links to papers, articles and websites Tutorials – guidelines for tutorial activities and specific supporting resources Practical exercises – guidelines for practical activities and specific supporting resources (texts, articles, websites) Group project – detailed instructions and supporting websites to guide students in navigating the challenges of working on a group project. These will include information on working in teams, as well as some literature on the research topic. Essential and recommended texts are in the Reserve Section, Alma Jordan Library, or are available via links in the course outline and myeLearning. Essential texts Sheppard, CRC, Davy SK, Pilling GM. 2009. The Biology of Coral Reefs. Oxford University Press. ISBN 978-0-19856636-6 Humaan P & DeLoach N (2002) The Reef Set: Reef Fishes, Reef Creatures and Reef Coral. 2nd Edition. New World Publications, 1250 p. ISBN:1878348337, ISBN-13:978-1878348333. Supplementary texts 3 Conditions permitting, there may be a weekend field trip, in which case two practical sessions will be combined into that single trip. 184 Rohwer F, Youle M, Vosten D (2010) Coral Reefs in the Microbial Seas. Plaid Press ISBN-10: 0982701209, ISBN13: 978-0982701201. Useful websites NOAA s Coral Health and Monitoring Program at http://www.coral.noaa.gov: this website, with its many links is a good entry point into online information on coral reefs. ADDITIONAL INFORMATION Refer to the Life Sciences Undergraduate Handbook for general guidelines for teaching, assessment, student conduct, essential skills, and support at http://sta.uwi.edu/fst/lifesciences/documents/handbook.pdf. Refer to the general information and general regulations in the Faculty of Science and Technology Undergraduate Booklet available from http://sta.uwi.edu/resources/documents/facultybooklets/ScienceTechUndergrad.pdf. Attendance: Students are reminded that they must attend and report satisfactorily on a minimum of 75% of the practical sessions and tutorials. Failure to do so will result in debarment from the final examination (Extracts from Examination Regulations for First Degrees, Associate Degree, Diplomas and Certificates, section II (18) available from http://sta.uwi.edu/resources/documents/Exam_and_GPA_regulations.pdf). Medical and other excuses: Any student who misses a class, test or assignment is advised to consult immediately in person or by email with the teaching assistant or course coordinator regarding reasons for their absence and recoup options. Absence must be explained in writing within 7 days of the missed session by either a written/emailed excuse sent to the TA or course coordinator, and/or a medical certificate submitted to the Health Service Unit and copied to the secretary, main office, Department of Life Sciences. The depart e t s op should ha e appe ded to it i for atio o courses affected, course coordinator or TA responsible for those courses, and course events involved. Make-up sessions: As a general principle, medical or other e uses a o l e use a stude t s prese e at an assigned time. Students must still complete the assigned work (make-up lab report or make-up test) in order to obtain the marks for that item of course work. The student is responsible for liaising with the course coordinator or teaching assistant to ensure the assigned make-up exercise is completed. Any student who was inexcusably absent from a practical or test, or who does not submit a practical report (initial or makeup) will receive 0% for that exercise. Note that a field trip cannot be repeated for a make-up opportunity, so you should make every effort to attend field trips. Late submissions: A penalty at 10% of mark per day late will be applied unless formal approval for late submission is given by the teaching assistant or course coordinator using the guidelines above for medical or other excuses. Assignment submission: Hard copy reports must be submitted into the labelled deposit box for your bench/demonstrator in the outer Zoology office, and the student list must be signed. Submissions anywhere else will not be assessed. Reports will be returned at a time arranged by the TA or demonstrator. We are not responsible for reports left uncollected or submitted inexcusably late. Soft copy reports or other assignments must be submitted through myeLearning as directed for each assignment. Academic dishonesty: Academic dishonesty, which includes plagiarism and cheating, is an extremely serious academic offense, and carries penalties varying from failure in an assignment to possible suspension from the university. Students are hereby informed that plagiarism is forbidden, and all unsupervised coursework 185 items must be accompanied by a completed plagiarism declaration form in order to be assessed. Specific items may require submission through Turnitin on myeLearning. Refer to the University Regulations on Plagiarism available from http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf. Safety in the laboratory and field: Medical data and emergency contact forms must be completed in the first laboratory session when safety briefings will be given. Standard laboratory protocols for conduct and dress code are in effect once you are present in a Life Sciences laboratory (Department of Life Sciences Undergraduate Student Handbook section 8). The dress code includes mandatory laboratory coats, adequate clothing to protect the body, and closed shoes. NO sandals, slippers or open-toed shoes will be allowed in the laboratory or in the field. Marks will be deducted for non-adherence to these protocols. Field work: Details are given in section 8 of the Department of Life Sciences Undergraduate Student Handbook http://sta.uwi.edu/fst/lifesciences/documents/handbook.pdf. Field trips are specified on the attached course calendar so you can be adequately prepared. You will not be allowed on the field trip if a medical data form has not been submitted. If you have a disability and have not disclosed the nature of your disability and the support you need, you are invited to contact the Academic Support/Disabilities Liaison Unit (ASDLU) of The University of the West Indies, St. Augustine Campus. Phone: 662-2002 extension 83866, 83921, 83923 and 84254; Fax: 662-2002 extension 83922; Email: [email protected] ; http://sta.uwi.edu/asdlu/forms.asp. HOW TO STUDY FOR THIS COURSE Firstly, this is not a course where you can rote-learn factual information at the last minute for a test, or prepare simplistic essays copied off the Internet for assignments. It requires familiarity with a wide range of topics, informed discussion of broad, complex issues and application of critical thinking skills. You should attend all lectures, tutorials and practical sessions since satisfactory attendance (>75%) is required by the UWI regulations. Attendance records will be taken at all sessions. Students who attend regularly perform better than those who do not. You should attend to obtain the full alue of the le turer s prese e. Le turers routi el s thesise a d su arise large a ou ts of i for atio , and prioritise, evaluate, discuss and explain this information. They provide the conceptual framework of the course, which you must then fill in with details and examples through independent study. You should prepare before classes so you can clarify any difficult points with the lecturer, or engage in meaningful discussion of any controversial topics. This will enhance and deepen your understanding as well as contribute to course assessment in tutorials and practical sessions. This is your opportunity for continuous assessment to monitor your progress and to accumulate coursework marks incrementally. You should review learning outcomes for each topic or activity, and make sure you understand and can actually achieve those outcomes. If you cannot, please clarify with the lecturer. You must make thorough use of the resources provided (e.g., textbooks, resouces on myeLearning) from the first week. You should check into myeLearning frequently to review updated materials, assignments, notices and activities. In addition to timetabled contact time, you are expected to devote additional hours of independent study: reviewing class materials and notes, reading/viewing/using resource materials, completing assignments, and preparing for assessments. You are strongly encouraged to make your own notes from lectures, textbooks and other source materials throughout the semester to improve your skills of comprehension, organisation, planning and writing. Working in study groups is strongly supported for those who learn best this way, as it shares workloads, builds camaraderie and social skills; however, please do not rote-learn prepared essays, a d al a s dou le he k the a ura of our olleagues ork. You are encouraged to interact regularly with staff, even outside of the assigned class times to ensure prompt, satisfactory resolution of any problems and to monitor progress. Attendance is mandatory for 186 lectures, tutorials and practical sessions. Prior preparation using online course materials is strongly advised to be able to fully participate in activities, and obtain the full value of the sessions. Thorough use should be made of the resources provided, and students are strongly advised to become familiar with them and start utilising them from the first week. Regular updates on course progress and materials are also highly recommended and you should be checking into myeLearning on a frequent, regular basis to review materials, assignments and activities. Some online activities will contribute to your final grade (see course assessment). Students are encouraged to interact regularly with staff on their projects, even outside of the assigned laboratory times, to ensure prompt, satisfactory resolution of any problems, and to monitor progress. Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 187 COURSE CALENDAR Wk Date Lecture topics Practical dates and topics 1 M18.1 Introduction – location and importance of coral reefs of the Caribbean T19.1 Due dates DP 2 W20.1 Coral reef geography – definition of coral reef, reef types and formation M25.1 Marginal coral reefs and other coral communities W27.1 Coral reef taxonomy – main reef-building organisms in the Caribbean DP 3 M1.2 T2.2 Coral reef taxonomy – other important coral-reef-associated taxa DP 4 Taxonomy of coral reef organisms W3.2 Coral biology 1 – anatomy and reproduction M8.2 Coral biology 2 – population ecology of corals (Carnival Monday, no lecture) W10.2 Geology of Caribbean coral reefs TS Practical 1 (2.5%) TS 5 M15.2 T16.2 Coral biology 3 – nutrition, calcification and growth DP 6 W17.2 Coral reef dynamics – reef-building processes, organisms JAI M22.2 Coral reef dynamics – carbonate budget W24.2 Coral reef dynamics – environmental controls on reefs (physical processes and zonation) JAI 7 M29.2 Coral reef dynamics – ecological relationships on the reef (competition, aggression, symbioses) W2.3 Coral reef dynamics – ecological succession M7.3 Coral reef dynamics – energy and materials; connectivity and reef resilience Coral reef health 1 (methods) Practical 2 (2.5%) T1.3 JAI 8 Practical 3 (2.5%) JAI W9.3 9 M14.3 Importance of coral reefs – tourism, recreation and ecosystem services Importance of coral reefs – biodiversity, fisheries and marine natural products JAI T15.3/S19.3 Coral reef health 2 (field) W16.3 10 M21.3 JAI W23.3 Valuation of coral reefs – use values and the total economic value; option values and non-use values Practical 4 (2.5%) Threats to coral reefs – natural disturbances 188 Threats to coral reefs – anthropogenic impacts (water pollution and climate change) 11 M28.3 Threats to coral reefs – reef responses (phase shifts, bleaching and disease) (Easter Monday, no lecture) JAI Coral reef health 3 (data) W30.3 12 T29.3 M4.4 Protecting coral reefs – assessment and monitoring of coral reef health and function Protecting coral reefs – mitigation and management (reef restoration) Research report (30%) JAI W6.4 Protecting coral reefs – mitigation and management (Caribbean reef management programmes, including MPAs) T12.4 13 T12.4 Tutorials THE UNIVERSITY OF THE WEST INDIES, ST. AUGUSTINE FACULTY OF SCIENCE & TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE DOCUMENTATION Course code: BIOL3462 Course Title: THE ECOLOGY OF FRESHWATERS Credits: 3 Level: 3 Semester: 2 Pre-requisites: Either BIOL1262 Living Organisms I and BIOL1263 Living Organisms II or BIOL1261 Diversity of Organisms OR (BIOL1065 Diversity of Plants and Animals and AGRI1012 Microbiology) AND (BIOL2XXX Fundamentals of Ecology OR BIOL1462 General Ecology and Biometry). Anti-requisite: BIOL2062 Freshwater Biology COURSE DESCRIPTION: This course provides an overview of characteristics, biodiversity and ecology of freshwater systems, e.g. rivers, lakes, wetlands, and other low salinity inland aquatic environments. The course will cover the characteristics and variety of freshwater systems; the diversity, biology and ecology of living organisms found associated with these systems; the structure and function of freshwater communities and 189 ecosystems; threats to freshwater systems and management strategies to provide sustainable benefits for ecosystems and human wellbeing. Students are expected to have a basic foundation in ecology and biodiversity. In addition to providing a foundation of theoretical knowledge, this course will emphasise practical skills and expose students to field and laboratory approaches for studying freshwater systems. It is an interactive ‘hands-on’ course where students are expected to prepare, participate and perform in an active way to engage with the content in a variety of ways. Assessment is designed to encourage students to work continuously with the course materials, explore and critically analyse research in this rapidly developing field. PURPOSE OF THE COURSE/RATIONALE: This is a core course for the Ecology & Environmental Biology specialisation in the Department of Life Sciences and is an essential area in environmental sciences, environmental biology and environmental management. It also broadens the scope of studies in biology, plant biology and zoology. Freshwater systems are diverse environments that support unique communities of organisms and provide invaluable ecosystem services for human survival and well-being. At the same time they are heavily impacted by humans since they integrate all activities on land, in the water and even the atmosphere. Understanding of these systems is necessary for their effective management. INSTRUCTOR INFORMATION: Name of course coordinator: Mary Alkins-Koo Office address and phone: Room 218, Zoology offices, Floor 2, New wing, Natural Sciences Building Email address: [email protected] Office hours: Mondays 10-11am, Fridays 11-12am Preferred method of contact: Email Communication policy: Students should use their UWI email account for communication and can expect a response within 48 hours. LETTER TO THE STUDENT: Welcome to ‘The Ecology of Freshwaters’. In this course you will be introduced to freshwater systems which provide essential water resources and food, they moderate the impact of natural disasters and provide many other ecosystem services essential for human survival and wellbeing. The course will be taught in blended mode comprising both face-to-face and online delivery. You are expected to engage with the materials provided online, in face-to-face lectures and tutorials as well as explore and interact with these to develop the essential skills of critical thinking (clarity, accuracy, relevance, logic, breadth, depth, precision, significance, completeness and fairness). Activities will be diverse and you are strongly encouraged to ask questions, offer new ideas, problem solve and think innovatively to maximise the value of your learning experience. Please read the Course Outline carefully and keep the Course Schedule close to hand as it contains all the activities and assessments during the semester. In particular take note of the policy on plagiarism and attendance requirements. 190 We are here to ensure your success in this course and at UWI generally and encourage you to come to us with any academic or other challenges you may face that could affect your attendance and performance. I look forward to meeting each of you and engaging in productive stimulating discussions over the coming semester. Dr Mary Alkins-Koo. Course Coordinator CONTENT: Topics will include: Origins and distribution of freshwaters Physicochemical characteristics of freshwaters Freshwater habitats and biodiversity Ecology of running waters Ecology of standing waters Ecology of freshwater wetlands Human interactions with freshwater systems Investigating freshwater systems GOALS/AIMS: This course aims to Introduce students to the characteristics and diversity of freshwater systems Develop a student’s competence in the methods and approaches for research in freshwater systems Expand a student's knowledge of the diversity and biology of living organisms by reference to freshwater organisms in particular Review the ecology of a range of freshwater systems stressing general concepts that allow for an understanding of their biodiversity, community structure and function Evaluate the range of benefits derived from these systems, how humans interact with them, and suggest approaches for managing and living sustainably with such ecosystems. GENERAL OBJECTIVES: The general objectives of this course are to provide course materials, activities and assessment using both face-to-face and online modalities facilitate opportunities for students to engage with general concepts and specific examples to develop a comprehensive understanding of the nature of freshwater systems and their biodiversity guide students in critically analysing and exploring questions in freshwater biology and ecology using investigative hands-on approaches facilitate discussion on the relationship between freshwater systems and human wellbeing and solutions for ensuring sustainable provision of such benefits LEARNING OUTCOMES: At the end of this course students should be able to 191 Give examples of the diversity of freshwater systems, compare them and discuss their distinguishing characteristics Describe the physicochemical characteristics of a range of standing and running waters and discuss and evaluate the factors which determine these physicochemical features Review and evaluate the methods used for measuring basic physicochemical parameters and sampling the biota in a range of freshwater systems in a variety of contexts Give examples of the major groups of freshwater flora and fauna and describe their biology (classification, distinguishing features) and ecology (habitats, adaptations, interactions) Discuss the factors that determine the distribution and abundance of freshwater biota in standing and running waters Discuss the physicochemical and biotic factors that influence the structure and function of freshwater communities Review concepts and hypotheses accounting for the structure of ecological communities in standing and running waters and their functioning in terms of transfer of energy and nutrients List the values of freshwater systems, describe the range of human uses of these systems, and discuss human impacts on freshwater environments, communities and ecosystems Propose general approaches for management of these systems based on a scientific understanding of these systems. COURSE ASSESSMENT: Assessment will be based on a student’s final mark from the coursework components below. Description Component % Final Grade Final exam Coursework 50% 50% 2-hour written exam with 5 essay questions Broken down as follows: 2 tests (each 10%) based on multiple choice, true/false, short response questions or essay Performance in face-to-face and online discussions and activities Performance and reporting of 4 practical exercises In-course tests Tutorials, online activities Practicals 20% 10% 20% ASSIGNMENTS: 2 coursework tests (20%): 2 tests (each 10%) each 50 minutes duration including multiple choice, true/false, short response questions Tutorials, online activities (10%): Continuous evaluation of participation and performance and contributions throughout the semester (discussions, wiki/ glossary, blogs) Practicals (20%): Practical performance and reporting of 4 practical exercises during the semester EVALUATION: 192 Feedback on the course will be obtained informally from students on an ongoing basis by regular interactions and meetings among students, demonstrators, teaching assistants and the Course Coordinator in practicals and tutorials. Formal feedback will be via election of Class Representatives who sit on the Departmental StudentStaff Liaison Committee meetings held twice during the semester. Class reps will channel both concerns and commendations to the meeting as guided by the Department’s Standard Operating Procedures. Formal evaluation of the entire course will be accomplished via a UWI Course Evaluation questionnaire administered anonymously and confidentially at the end of the semester. All feedback will be considered on an ongoing basis and corrective action or adjustments made or discussed with students promptly or incorporated the following year. TEACHING STRATEGIES: Contact hours: 36 h Lectures: 22 h Tutorials: 6 h Practicals: 8 h (4 x4 h = 16h practicals) Lectures: Lectures will provide valuable synthesis and evaluation of the growing body of available information, update current issues and events, and prioritise content relevant to course assessment. Practicals: Fortnightly practicals will provide hands on experience for students to gain skills required for conducting well designed field and laboratory sampling and experiments in a variety of situations; to problem solve and trouble shoot in real-life situations; and gain a better appreciation of the environment and the organisms of freshwater systems. Participation and performance in the practicals will be assessed as well as reports based on the practical activities. Tutorials: Tutorials will cover course topics in a highly interactive format using a variety of collaborative active learning techniques. In addition to clarifying details of course content, specific transferrable skills will be addressed e.g. essay and report writing, critiquing and oral presentation. myelearning: The online teaching tool, myeLearning, will be used extensively during this course for communication among students and staff (email, discussions) official posting of important notices (coursework assessment notices, instructions, and in-course results) provision of course details, lecture notes, practical guides, tutorial briefings provision of recommended resource materials and links to resources on specific websites self-test quizzes, questionnaires, surveys interactive activities such as discussions, wikis, glossaries, blogs RESOURCES: Most resources are available myeLearning including Lecture presentations including learning objectives, summaries, recommended readings and links to papers, articles and websites with interactive resources and videos for those who prefer to learn using these modalities ‘General Resources’ provides direct links to general websites, articles and publications relevant to the whole course Tutorials may have specific supporting resources particularly on skills development 193 Essential and Recommended texts are in the Reserve Section, UWI Main Library or via links in the course outline and myelearning Readings: Moss, B. 2010. Ecology of freshwaters: a view for the twenty-first century. Hoboken, NJ: WileyBlackwell. QH541.5.F7 M67 2010 Closs, G., B. Downes, & A. Boulton. 2004. Freshwater Ecology: A scientific introduction. Malden, MA: Blackwell Pub. QH541.5 F7 C66 2004 Lampert, W. & U. Sommer. 2007. Limnoecology: The ecology of lakes and streams. New York: Oxford University Press. QH96.57.A1 L3513 2007 Web links: Water on the Web http://www.waterontheweb.org/ COURSE CALENDAR: WEEK 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. PRACTICALS TUTORIALS LECTURES 50. Course Introduction 51. Origins and distribution of freshwaters 52. Physicochemical parameters 53. Physicochemical parameters 54. Freshwater habitats and biota 55. Diversity and scale in running waters Tutorial 1 Practical 1: Investigating freshwaters 56. 57. 58. 59. Conditions and microdistribution Longitudinal patterns in rivers River ecosystems River Continuum Concept Tutorial 2 60. 61. 62. 63. Floodplain Rivers Flood-Pulse Concept Diversity of standing waters Stratification and mixing Tutorial 3 64. 65. 66. 67. Lake communities Lake ecosystems Tropical lakes Freshwater wetlands Tutorial 4 68. Temporary waters 69. River regulation 70. Watershed processes & management 71. Pollution 72. Freshwater ecosystems and human well being Assignments Practical 2: River ecology In-course Test 1 Practical 3: Pond ecology Online activity Practical 4: Data analysis & report writing Tutorial 5 Tutorial 6 In-course Test 2 194 26. 73. Revision/review ADDITIONAL INFORMATION: Refer to the Life Sciences Undergraduate Handbook for general guidelines for teaching, assessment, student conduct, essential skills, and support at http://sta.uwi.edu/fst/lifesciences/documents/handbook.pdf . Refer to the General Information and General Regulations in the Faculty Booklet available from http://sta.uwi.edu/resources/documents/facultybooklets/ScienceTechUndergrad.pdf . Students are reminded that they must attend a minimum of 75% of the practical sessions and tutorials. Failure to do so will result in debarment from the final examination. As a general principle, medicals or other excuses may only excuse a student’s presence at an assigned time. Students must still complete the assigned work (make-up lab report or make-up test) in order to obtain the marks for that item of coursework. The student is responsible for liaising with the Course Coordinator or Teaching Assistants to ensure the assigned make-up is completed. Students are hereby informed that plagiarism is forbidden and all unsupervised coursework items must be accompanied by a Coursework Accountability Statement in order to be assessed. Specific items may require submission through Turnitin on myeLearning. Refer to ‘University Regulations on Plagiarism’ available from http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf HOW TO STUDY FOR THIS COURSE: Attendance is mandatory for lectures, tutorials and practicals. Prior preparation is strongly advised to able to fully participate in activities and obtain the full value of the sessions. Thorough use should be made of the resources provided and students are strongly advised to become familiar with them and start utilising them from the first week. Regular updates on course progress and materials are also highly recommended and you should be checking into myelearning on a frequent regular basis to review materials, assignments and activities. Students are encouraged to interact regularly with staff on their projects, even outside of the assigned tutorial times to ensure prompt, satisfactory solution of any problems and to monitor progress. Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 195 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 MAK: 18 February 2013 THE UNIVERSITY OF THE WEST INDIES, ST. AUGUSTINE FACULTY OF SCIENCE & TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE DOCUMENTATION Course code: BIOL3465 COURSE TITLE: Tropical Forest Ecology and Use Credits: 3 Level: 3 Semester: II Pre-requisites: Either BIOL2XXX Biostatistics and BIOL2XXX Fundamentals of Ecology or BIOL1462 General Ecology and Biometry Anti-requisite: BIOL3464 -Tropical Forestry Ecology and Management COURSE DESCRIPTION: This course is designed to expose students to the tropical forest ecology and how it influences the human use of tropical forests such as timber production and conservation. The course is organised into background 196 lectures and tutorials covering general and specific concepts in tropical forest ecology and management. In tutorials students are expected to prepare, participate and perform in an active way in order to engage with the content. Assessment will be based largely on in course tests and a final theory exam. PURPOSE OF THE COURSE/RATIONALE: This is a core course for the Environmental Biology minor in Life Sciences that will provide fundamental knowledge and concepts in tropical forest ecology that students can apply in most areas they may encounter in Environmental Biology. At the end of the course the students will be able to understand the issues in management of tropical forest ecosystems and how they respond to human manipulation. INSTRUCTOR INFORMATION: This course will be taught by a team of lecturers, Teaching Assistants and demonstrators. Office hours are posted on Departmental notice boards and the main Life Sciences office. Name of course coordinator: Mike Oatham Office address and phone: Rm 222, Old Wing, Natural Sciences Building (ext 83088) Email address: [email protected] Office hours: Preferred method of contact: Email Communication policy: Students should use their UWI email account for communication and can expect a response within 48 hours. LETTER TO THE STUDENT: Welcome to ‘Tropical Forest Ecology and Use’. In this course you will be challenged to develop the fundamental knowledge, skills and attitudes of a practising tropical forest ecologist or forester. This is a theory course that will require commitment and participation on your part to successfully engage in the content and to develop the essentials skills of critical thinking (clarity, accuracy, relevance, logic, breadth, depth, precision, significance, completeness and fairness). You are strongly encouraged to ask questions, offer new ideas, problem solve and think innovatively to maximise the value of your learning experience. In addition, teamwork, organisation and time management will be necessary personal attributes to bring to the course. CONTENT: Topics will include The characteristics of tropical biomes and tropical forests. Nutrients and tropical forests. Regulation of biodiversity in tropical forests. Succession and regeneration in tropical forests. Disturbance in tropical forests. History of forestry in the wet tropics. Abiotic and biotic problems of tropical forestry. Tropical forestry and the goal of sustainable yield. Plantation forestry in the wet tropics. The social dimension of tropical forestry. The future for tropical forest management. General transferable skills 197 Oral and written communication; manipulation, observation and record-keeping; working with technology, information and numbers; managing time, people and projects; problem identification and solving; effective thinking, learning and research; innovation, design and evaluation. Evaluating, managing and communicating information in tropical forest ecology Reporting ethics including plagiarism Managing, analysing and presenting data GOALS/AIMS: This course aims to build a range of competencies in transferrable skills as applied to Tropical Forest Ecology expose students to the general concepts and knowledge used for understanding Tropical Forest Ecology introduce specialist analytical skills needed to manage tropical forests GENERAL OBJECTIVES: The general objectives of this course are to provide opportunities to develop transferrable skills within a specific context provide fundamental preparation and training in general practical and research skills introduce specialist practical and research skills and approaches inculcate a culture of quality and responsibility in conducting research enhance students’ capacity to work effectively in real life situations LEARNING OUTCOMES: At the end of this course students should be able to Describe the major ecological processes shaping tropical forests. Describe the uses of tropical forests, the conflicts and synergies in these uses and will understand the ecological effects of the different uses and their conflicts. Describe how the formulation of forest management regimes can cater to different uses particularly timber production. Describe how local and international economic pressures are bought to bear on forest management in the tropics. Effectively find, evaluate and manage published information from a variety of sources. Use spreadsheet, word processing and presentation software to carry out tasks related to critically assessing and synthesizing reports from the scientific literature. Review basic ethical principles for appropriate scientific conduct Function effectively in real life situations by integrating knowledge, skills and attitudes with innovation and problem-solving approaches COURSE ASSESSMENT: Assessment will be based on a student’s final mark from the coursework components below. Component % Final Grade In-course tests/ quizzes Practical Reports Final Theory Exam 100% broken down as follows: 30% 30% 40% ASSIGNMENTS: 198 Topic Quizzes (30%) made up of: 10 Topic Quizzes (3% each) each week during the semester from week 2 to week 11 Practical Reports (30%) made up of: Skills Test (week 2) (5%); 5 Practical Reports (5% each) due in weeks 5, 7, 9, 11 & 13 EVALUATION: Feedback will be obtained informally from students on an ongoing basis by regular interactions and meetings among students, demonstrators, teaching assistants and the Course Coordinator in practicals and tutorials. Formal feedback will be via election of Class Representatives who sit on the Departmental StudentStaff Liaison Committee meetings held twice during the semester. Class reps will channel both concerns and commendations to the meeting as guided by the Department’s Standard Operating Procedures. Formal evaluation of the entire course will be accomplished via a UWI Course Evaluation questionnaire administered anonymously and confidentially at the end of the semester. All feedback will be considered on an ongoing basis and corrective action or adjustments made or discussed with students promptly or incorporated the following year. TEACHING STRATEGIES: Delivery: contact hours (39 credit hours): Lectures/Tutorials: 24 h (24 credit hours) Practical work: 30 h (15 credit hours) Lectures and tutorials will provide introduction to specific topics and allow for discussion in an interactive setting Practicals will provide hands-on training in general approaches and specific techniques in the form of laboratory sessions or field trips RESOURCES: Lectures and Tutorials Lecture room with internet connection and multi-media projector Practicals Transport to field sites for practical work Field equipment such as 50m tapes, 1m dress-maker’s tapes, compasses, binoculars and first aid kits COURSE CALENDAR: Week Topic 1 Lectures/Tutorials Global Distribution of Tropical Forests Climate and altitude Other Tropical Ecosystems 199 Practicals Assignments Week Topic Lectures/Tutorials 2 Local Distribution of Tropical Forests Soils Fire 3 Changes in Tropical Forest Distribution 4 Physiognomy and Floristics of Tropical Forests 5 Competition in Tropical Forest Ecosystems Tropical ecosystems through time Global climate change and tropical forests Structure of tropical forest ecosystems Floristics of tropical forest ecosystems Life history strategies of tropical rainforest plants Tropical Forest Dynamics Herbivory and Predation Mutualisms and Commensal Reasons for diversity in tropical forests Monodominance in tropical rainforests. A Trinidad example Loss and degradation of tropical forests Importance of tropical forests to human society History of tropical forest management Basic silviculture terms and practices Replacement and clearing systems of timber production Natural regeneration and restoration systems of timber production Biodiversity conservation in production forests Sustainable forest management in the Victoria-Mayaro Forest Reserve 6 Other Biotic Interactions in Tropical Forests 7 Biodiversity levels and Maintenance 8 Tropical Forests in the Anthropocene 9 History of Tropical Forest Management 10 Timber Systems Production 11 Timber Production and Biodiversity Conservation Practicals Assignments Field Skills for Practical Skills Tropical Forest Test Ecology Topic Quiz 1 200 Topic Quiz 2 Biotic and Abiotic Topic Quiz 3 Characteristics of Tropical Forest Topic Quiz 4 Practical Report 1 Structure of Topic Quiz 5 tropical forest ecosystems Topic Quiz 6 Practical Report 2 Loss of tropical Topic Quiz 7 forests? Topic Quiz 8 Practical Report 3 Plantation natural structure diversity and Topic Quiz 9 forest and Topic Quiz 10 Practical Report 4 Week Topic 12 Lectures/Tutorials Other Tropical Forest Services Practicals Assignments Carbon Sequestration Carbon and REDD sequestration in Watershed protection tropical forests 13 Practical Report 5 ADDITIONAL INFORMATION: Please refer to the Life Sciences Undergraduate Handbook for general guidelines for teaching, assessment, student conduct, essential skills, and support at http://sta.uwi.edu/fsa/lifesciences/documents/handbook.pdf . Refer to the General Information and General Regulations in the Faculty Booklet available from http://sta.uwi.edu/fsa/documents/UGFacultyBooklet.pdf . Students are reminded that they must attend a minimum of 75% of the practical sessions and tutorials. Failure to do so will result in debarment from the final examination. As a general principle, medicals or other excuses may only excuse a student’s presence at an assigned time. Students must still complete the assigned work (make-up lab report or make-up test) in order to obtain the marks for that item of coursework. The student is responsible for liaising with the Course Coordinator or Teaching Assistants to ensure the assigned make-up is completed. Students are hereby informed that plagiarism is forbidden and all unsupervised coursework items must be accompanied by a Coursework Accountability Statement in order to be assessed. Specific items may require submission through Turnitin on myeLearning. HOW TO STUDY FOR THIS COURSE: Attendance is mandatory for lectures, tutorials and practicals. Full use should be made of the resources provided and students are strongly advised to become familiar with them and start utilising them from the first week. Students are encouraged to interact regularly with their lecturer on their projects, even outside of the assigned tutorial times to ensure satisfactory solution of any problems and to monitor progress. Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 201 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 THE UNIVERSITY OF THE WEST INDIES ST. AUGUSTINE FACULTY OF SCIENCE AND TECHNOLOGY DEPARTMENT OF LIFE SCIENCES TITLE: BIOL 3466- Coastal Ecosystems and Resource Management CREDITS: 3 LEVEL: Undergraduate -Year III SEMESTER: II PRE-REQUISITES: BIOL 3XXX Marine Ecology & Oceanography ANTI-REQUISITE: BIOL3461 Coastal Ecosystem Management OFFERING DEPARTMENT: Department of Life Sciences, Faculty of Science and Agriculture, The University of the West Indies, St. Augustine. COURSE COVERAGE: This course will provide students with an understanding of the characteristics of the major coastal ecosystems of the Caribbean and adjacent regions. It emphasises the ecological processes that determine resource values and functions and highlights the reasons for habitat and resource degradation. The course examines the principles and practices of coastal ecosystem management and reviews the major coastal management initiatives in the region. The course also includes field surveys which cover many of the issues covered in the lectures. 202 PURPOSE OF THE COURSE /RATIONALE: This course will provide students with an understanding of the characteristics of the major coastal ecosystems of the Caribbean and adjacent regions. It emphasises the ecological processes that determine resource values and functions and highlights the reasons for habitat and resource degradation. The course examines the principles and practices of coastal ecosystem management and reviews the major coastal management initiatives in the region. The course also includes field surveys which cover many of the issues covered in the lectures. Students are introduced to ecosystems as resources and some basic management principles are also introduced. For each ecosystems- the goods, services and attributes are described. Students are exposed to a number of management tools and applications using relevant Caribbean examples. INSTRUCTOR’S INFORMATION Dr. Judith Gobin (Coordinator) Room 222, Natural Sciences Building, UWI- Life Sciences Phone: 868-662-2002 Extn. 82046; Fax: 868-663-5241 Email: [email protected] Preferred contact – By Email Office-Contact hours (Coordinator) Day Monday Tuesday Wednesday Thursday Friday Time 10.00 am to 4.00 pm 10.00 am to 4.00 pm 10 .00 am to 4.00 pm 10.00am to 12.00am LETTER TO THE STUDENT Dear Students, I would like to extent a very warm welcome to you as we get prepared for the start of BIOL 3461 – Coastal Ecosystems Management. I am Dr Judith Gobin, your Course Coordinator. This course is fully supported through myelearning, which we update regularly. Please feel free to contact me (course teacher)or your TA/demonstrator for any academic help and assistance. Please know that we are here to support your learning and success as a student in FSA. This course is designed to provide you with an understanding of a number of coastal resources and their significance in terms of their resource values. Another goal of the course is to teach students 203 how to critically assess environmental problems and how to devise appropriate management strategies. A few points to note: The Course Schedule (dates/activities) is available on the course page and should be downloaded for your reference and continued guidance, as soon as you have access. Please take the time to read the policy on plagiarism. Once again, I would like to take this opportunity to welcome you and look forward to some healthy interactions over the course of the next semester. So, let’s get your feet wet! Your Course Coordinator Dr Judith Gobin. CONTENT This course introduces students to a number of coastal ecosystems and their associated resources. We begin with Mangroves, Coral Reefs, the sea and sandy beaches. We then look at impacts of a variety of activities on the ecosystems and their resources. We also evaluate the best management strategy or option which may be applied to sustain the resource values. COURSE GOALS: This course aims to: Provide students with an understanding of the characteristics of the major coastal ecosystems of the Caribbean and adjacent regions. Re-emphasise the ecological processes that determine resource values and functions and highlights the reasons for habitat and resource degradation. Examine the principles and practices of coastal ecosystem management and reviews the major coastal management initiatives in the region. Include field surveys which cover many of the issues covered in the lectures. The emphasis throughout the course will be on Caribbean coastal ecosystems, especially those of Trinidad & Tobago. COURSE OBJECTIVES: Upon successful completion of this course, the student will be able to appreciate and understand as well as have gained practical experience under the following headings: Coastal Resources: - this by examination of the natural resources associated with beaches, wetlands, estuaries, harbours and other shoreline features. Development Impacts and Pollution Ecology: - this by examination of the kinds of development impacts and pollution affecting coastal ecosystems, especially organic, oil, pesticide, heavy-metal, physical and thermal pollution, their sources, effects and remedies. Coastal zone and Resource Management: - this by Coastal surveys, environmental monitoring, water quality criteria, zoning, legislation and enforcement. 204 Resource Use, Protection and Conservation: - this by Coastal zone use, protection and conservation. Their purposes, criteria, development and management. LEARNING OUTCOMES: At the end of this course students will be able to: Skill outcomes: Interpret coastal and marine/environmental data from the field. Analyze and interpret field data using appropriate coastal zone management tools eg. baseline surveys, environmental impact assessments, management plans etc.. Actively seek and assimilate information from texts, assigned articles and reference texts. Dispositional outcomes: Describe he relevance and importance of the need for management of coastal zones and associated ecosystems . Ask questions or seek help from the instructors when material is difficult or confusing. ASSIGNMENT Students will have to submit a written report for each of six practical exercises. These are designed to relate to all of the major topics that are covered in the lectures. Students will also have two in-course tests, each covering specific areas of the course. These are designed to determine if the students can correctly recall all that was covered in the previous weeks of the course. The 1st is an 1 Essay question and the 2nd is a multiple-choice question exam. COURSE ASSESSMENT Incourse assessments consist of both theory and practical components, and together make up the 40% incourse marks. A student missing an incourse examination because of an illness or legitimate emergency will be allowed to do a makeup examination the date of which will be advertised and shall be within 7 days of the first test. In such a circumstance, the student should make every reasonable attempt to contact the instructors as soon as possible. While make-up exams will cover the same content area as a missed exam, the exam format and specific questions may be different. There shall be no makeup labs, students missing a practical exercise must submit a valid medical within 7 days of the practical. Students must attend 75% of their practicals. Failure to do so may result in the student being debarred from the final examination (Examination regulations Section II part 19 http://sta.uwi.edu/resources/documents/Exam_and_GPA_regulations.pdf). If you do have a disability and have not disclosed the nature of your disability and the support you need, you are invited to contact the Academic Support/Disabilities Liaison Unit (ASDLU) of The University of the West Indies (UWI), St. Augustine Campus. Phone: 662-2002 extension 83866, 83921, 83923 and 84254 Fax: 662-2002 extension 83922 Email: [email protected] http://sta.uwi.edu/asdlu/forms.asp Academic Dishonesty: 205 Academic dishonesty, which includes plagiarism and cheating, is an extremely serious academic offense and carries penalties varying from failure in an assignment to possible suspension from the University. http://sta.uwi.edu/fsa/lifesciences/accountability.pdf (Department of Life Sciences Handbook) ASSESSMENT Students would be assessed on both their practical and theoretical knowledge of the topics. Component % Final Grade Description Final exam 50% 2-hour written exam to answer 2 essay questions In-course tests 20% 2 tests (each 10%) . The 1st- a Research Essay and the 2nd- a multiple choice, true/false, short response question paper. Tutorials, online 10% Performance in interactive discussions and activities face-to-face and online 20% Performance and reporting of 5 practical exercises activities Practicals ASSIGNMENTS: Students will also have two in-course tests, each covering specific areas of the course. These are designed to determine if the students can correctly recall all that was covered in the previous weeks of the course. The 1st is an 1 Essay question and the 2nd is a multiple-choice question exam. (together total 20%) Students will have to submit a written report for each of four practical exercises. These are designed to relate to all of the major topics that are covered in the lectures. The practical assessment will consist of four (5hr) Laboratory exercises plus 2 Journals associated with the Labs. Together these account for 20% of the final grade. Practical write-ups are handed in at the end of each lab. (6.00pm) Journals are handed in 1 week after the Field trip. (4.00pm) EVALUATION: At the onset- students are asked to pop in to see the Course Co-ordinator or to make an appointment- to address any problems they may be having. 206 Dr. Gobin has an open-door policy (once on-campus and available). Chosen Class Representatives will attend meetings with the staff/student Liaison Committee, and give their feedbacks/comments on the course to the committee. Class Reps. are advised to address issues to me directly as soon as possible since there are only 2 meetings of this Committee per semester. This means issues may get delayed in being addressed. The UWI/IDU course evaluation forms at the end of the semester will be used to address and improve on the specific comments/feedback as best as possibly. TEACHING STRATEGIES: Course objectives will be achieved through the aid of the following learning activities: 1. Assigned readings 9. Class lecture and videos 10. Web page resources including published scientific papers 11. Practical exercises The material presented in all modes of instruction will be sufficient in content to allow students to have a good understanding of the topics. Class lectures will help to focus the student to some of the more important details of each topic. Assigned readings will serve supplement lectures. These include lectures notes, web based materials and original research papers on topics covered during lectures. We would also request that students post links to interesting articles for discussion in the forums in the course page. Contact hours (39 credit hours): Lectures: 23 h Tutorials: 6 h Practicals: 10 h (4 x 5hr = 20h practicals) RESOURCES Text books: Internet resources (as recommended bu the Co-ordinator) Designated readings: Lectures, powerpoints and an e-text are on my-elearning) OTHER RESOURCES: My-elearning (through my.uwi.edu) will be the main online resource. Discussion topics will be placed online for class comments. There is also a Facebook page- UWI Coastal Ecosystems. READINGS Instructors will periodically upload in my-elearning important research articles, review papers, book chapters and other related study materials. Students are expected to consult prescribed text books for relevant chapters for complete details and for preparation for examinations Students are expected to download those files or visit the suggested websites or read relevant book chapters/journal articles before they come for the following class. These were considered and included as portions of the syllabus and covered fully during examinations. 207 ADDITIONAL INFORMATION Additional information posted in the Department of Life Science hand book may be consulted for additional details regarding, Role of Lectures, Tutorials and Practical’s, Attendance, Online Teaching Resources, General safety guidelines in Lab and General Safety Practices Accident and incident reporting forms. HOW TO STUDY FOR THIS COURSE Understanding biological science involves understanding many difficult concepts and vocabulary, not just knowing facts. The student should know that the details to these concepts are important. While the student will have lecture outlines, they can only succeed in this class by making careful lecture notes and reading the corresponding material in the textbook before and after the lecture. The student should carefully review these lecture notes as often as possible. In addition, the students’ study activities should include: reviewing all of the internet resource materials provided, and making flashcards for some topics. The student should use these card for self-testing as often as possible. Students are recommended to establish study groups and study together. The students in these groups may test each other's knowledge and understanding of the information. They may also take turns teaching each other. Within these groups, students should attempt past paper questions and can also submit these to the lecturers for comments. Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 208 0-39 F3 Poor 0 BIOL 3XXX Management of Coastal Ecosystems WEEK LECTURES 1 Introduction to Coasts and Caribbean Coastal Ecosystems Beach structure and processes Beaches, Strand Communities & Resources 2 Coral Reefs and Resources/ Mangrove Area Resources Mangrove-Coral ReefSeagrass interactions Tutorial Coastal Development and Impacts Impacts of development on Corals, Mangroves and Seagrasses. 3 4 5 6 7 8 PRACTICALS Assignments Field trip Location: Port of Spain, North- west peninsula and North coast (JGobin) Concept & Nature of Coastal Pollution. Causes & Impacts of Oil Pollution(AM) Environmental Field trip Standards & Toxicity Location: North east Coast (Saturday) Testing Oil Residue Control in Trinidad Waters Tutorial Environmental Impact Assessments Legal Provisions for Coastal Management Resource Management Laboratory excercises Options & Practices Resource Inventories and Mapping, Data Collection and Analyses Tutorial Management Responses to Climate Change and Sea Level Rise, Hurricanes etc. 209 Test 1 9 Management Responses to over-exploitation Coastal Zone Laboratory excercises management and conservation Tutorial 10 Coastal Protected Areas and Marine Parks 11 Public Participation and Coastal Zone Management 12 13 . Tutorial Tutorial Test 2 JGobin 2013-02-15 210 THE UNIVERSITY OF THE WEST INDIES, ST. AUGUSTINE FACULTY OF SCIENCE & TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE OUTLINE COURSE CODE: BIOL 3468 COURSE TITLE: Biodiversity and Conservation NO. OF CREDITS: 3 LEVEL: 3 PRE-REQUISITE(S): BIOL2XXX Fundamentals of Ecology and either BIOL2XXX Biostatistics OR ESST2XXX Environmental Statistics OR BIOL1462 General Ecology and Biometry. ANTI-REQUISITES: BIOL3062 Conservation Biology COURSE DESCRIPTION: This course introduces students to one of the most important issues facing biologists and society at large today and in the coming decades: the enormous loss of biological diversity that accompanies the expansion of human populations. The objectives of this course are to provide students with an understanding of biodiversity, the threats to it and methods for preventing its loss. The perspective will be primarily biological, but social and economic aspects will be covered also. Because of the complexity of the issues involved, the course tries to foster interdisciplinary thinking and problem solving. This interdisciplinary approach to biodiversity problems is called Conservation Biology. Teaching for he course will be approached in a “hybrid online” manner with lectures presented online with recorded video and audio and the lecture contact periods given over to a mix of exercises such as debates, quizzes, roleplaying and question and answer sessions. Practical exercises involving field work, literature review and synthesis work and computer simulation/exercises will also be applied to gain more practical skills. COURSE RATIONALE: This is a core course for the Environmental Biology minor in Life Sciences that will provide fundamental knowledge and concepts in conservation of biodiversity that students can apply in most areas they may encounter in Environmental Biology. At the end of the course the students will be able to understand the issues in management of tropical biodiversity and how they respond to human manipulation. INSTRUCTOR INFORMATION: This course will be taught by a team of lecturers, Teaching Assistants and demonstrators. Office hours are posted on Departmental notice boards and the main Life Sciences office. Name of instructor(s): Mike Oatham (Coordinator) Office address and phone: Rm 222, Old Wing, Natural Sciences Building (ext 83088) 211 Email address: [email protected] Office hours: Preferred method of contact: Email Communication policy: Students should use their UWI email account for communication and can expect a response within 48 hours. CONTENT Topics will include: Types and distribution of biodiversity; Loss of biodiversity and its consequences; Endangered species; Population viability analysis and monitoring. Conservation practices for protected areas, biosphere reserves, Restoration ecology; Ex situ conservation strategies and population genetic considerations; Establishing new populations by translocation and reintroduction; Legal and institutional aspects: Land tenure systems and species and habitat protection; National conservation legislation; Conservation authorities and organizations; International programmes; international conservation treaties and conventions; Conservation education. General transferable skills Oral and written communication; manipulation, observation and record-keeping; working with technology, information and numbers; managing time, people and projects; problem identification and solving; effective thinking, learning and research; innovation, design and evaluation. Evaluating, managing and communicating information in tropical forest ecology Reporting ethics including plagiarism Managing, analysing and presenting data GOALS/AIMS The goals of this course are to describe what biodiversity is and where biodiversity is located globally and locally examine the reasons why biodiversity needs to be conserved expose students to the currently accepted approaches to conserving biological diversity provide opportunities to develop transferrable skills within a specific context provide fundamental preparation and training in general practical and research skills introduce specialist practical and research skills and approaches inculcate a culture of quality and responsibility in conducting research enhance students’ capacity to work effectively in real life situations 212 LEARNING OUTCOMES At the end of this course students should be able to: Describe the different levels of biodiversity and its global distribution. Describe where Trinidad and Tobago fits into the global biodiversity context. Assess how biological diversity is being lost around the world, how we know this is happening and which species and ecosystems are most vulnerable to extinction. Explain how describing the population dynamics of a species is critical to understanding if a species is in danger of extinction and how this knowledge will allow effective conservation actions to be developed for the species. Explain how in-situ conservation through protected areas is planned and executed. Describe what restoration ecology is and what attempts at restoration ecology have been made in Trinidad and Tobago Explain the basic principles of conservation genetics and how they are important to conservation of biodiversity Evaluate the legal and institutional measures put in place in Trinidad and Tobago for the conservation of biodiversity and the effectiveness of these measures in the current social and economic context Effectively find, evaluate and manage published information from a variety of sources. Use spreadsheet, word processing and presentation software to carry out tasks related to critically assessing and synthesizing reports from the scientific literature. Review basic ethical principles for appropriate scientific conduct Function effectively in real life situations by integrating knowledge, skills and attitudes with innovation and problem-solving approaches ASSIGNMENTS Topic Quizzes (30%) made up of: 10 Topic Quizzes (3% each) each week during the semester from week 2 to week 11 Practical Reports (30%) made up of: 4 Practical Reports (16%- 4% each) due in weeks 2, 5, 10, & 12 Conservation Video Group Project (14%) made up of: Concept of Video Project and Seminar (4%) Final Conservation Video Production (10%) COURSE ASSESSMENT Assess e t ill e ased o a stude t s fi al ark from the coursework components below. Component % Final Grade 100% broken down as follows: In-course tests/ quizzes 30% Practical Reports 16% Conservation Video Production 14% 213 Final Theory Exam 40% EVALUATION Feedback will be obtained informally from students on an ongoing basis by regular interactions and meetings among students, demonstrators, teaching assistants and the Course Coordinator in practicals and tutorials. Formal feedback will be via election of Class Representatives who sit on the Departmental StudentStaff Liaison Committee meetings held twice during the semester. Class reps will channel both concerns and commendations to the meeting as guided by the Department’s Standard Operating Procedures. Formal evaluation of the entire course will be accomplished via a UWI Course Evaluation questionnaire administered anonymously and confidentially at the end of the semester. All feedback will be considered on an ongoing basis and corrective action or adjustments made or discussed with students promptly or incorporated the following year. TEACHING STRATEGIES Delivery: credits: 3 as follows Lectures/Tutorials: 24 h (2 credits) Practical work: 24 h (1 credit) Lectures and tutorials will provide introduction to specific topics and allow for discussion in an interactive setting Practicals will provide hands-on training in general approaches and specific techniques in the form of laboratory sessions or field trips RESOURCES* Lectures and Tutorials Lecture room with internet connection and multi-media projector myeLearning page Practicals Transport to field sites for practical work Field equipment such as 50m tapes, 1m dress-maker’s tapes, compasses, binoculars and first aid kits READINGS Required: Primack R.B. 2010. Essentials of conservation biology. 5th Ed. Sinauer Associates, Sunderland, MA. 585pp. Supplementary: Meffe, G. K., and C. R. Carroll. 1997. Principles of Conservation Biology. 2nd ed. Sinauer Associates, Sunderland, MA. 673 pp. 214 COURSE CALENDAR* Topic Lectures Practicals Assignments Week 1. Why should we care about Conservation of Biodiversity? Introduction to Measurement and Representation Conservation Biology of Diversity: Ground flora of Conservation Values Tropical Forest. and Ethics Week 2. What is Biodiversity and Why do we have it? Biodiversity. What is Biodiversity? Biodiversity: Processes. Why so many Species? Practical Report 1. Topic Quiz 1 Week 3. Extinctions Biological Conservation Video Extinctions Productions. Presentation of Vulnerability to Extinction and the Evil Concepts Trinity Video Production Concept Seminars Topic Quiz 2 Week 4. Biodiversity Loss: Landscape scale processes Topic Quiz 3 Population Genetics Species Demography and Meta-populations Fragmentation and Biodiversity Conservation Conservation Genetics Introduction to worksheets. Population Genetics Genetics of Small Populations Problems of conserving small populations In situ and ex situ methods Week 5. Week 6. Endangered Species Conservation Week 7. Determining Conservation Actions Week 8. Restoration Ecology Week 9. Protected Areas for Conservation of Biodiversity Topic Quiz 4 Practical Report (Cons. Genetics) 2. Topic Quiz 5 Biological Conservation Video Topic Quiz 6 Conservation Triage: Defining conservation Productions. Work on Videos priorities Population Viability Analysis and Species Recovery Planning Topic Quiz 7 Restoration Ecology. Techniques Restoration Ecology. Objectives Protected Areas. Topic Quiz 8 Designing and Establishing Protected Areas Managing Protected Areas 215 Topic Lectures Week 10. Conservation of Biodiversity and Human Society Week 11. Conservation of Biodiversity at a National Level Week 12. National and Regional Conservation of Biodiversity Practicals Assignments Resource Use by Local Communities Economics of Biological Conservation Topic Quiz 9 Practical Report 3. Role of Institutions and Attitudes to Conservation: Campus Survey Policymaking in Conservation Environmental Law Topic Quiz 10 Biodiversity of Trinidad and Tobago and its Conservation Caribbean Conservation Practical Report 4 Week 13. Biological Conservation Video Productions ADDITIONAL INFORMATION Please refer to the Life Sciences Undergraduate Handbook for general guidelines for teaching, assessment, student conduct, essential skills, and support at http://sta.uwi.edu/fsa/lifesciences/documents/handbook.pdf . Refer to the General Information and General Regulations in the Faculty Booklet available from http://sta.uwi.edu/fsa/documents/UGFacultyBooklet.pdf . Students are reminded that they must attend a minimum of 75% of the practical sessions and tutorials. Failure to do so will result in debarment from the final examination. As a general principle, medicals or other excuses may only excuse a student’s presence at an assigned time. Students must still complete the assigned work (make-up lab report or make-up test) in order to obtain the marks for that item of coursework. The student is responsible for liaising with the Course Coordinator or Teaching Assistants to ensure the assigned make-up is completed. Students are hereby informed that plagiarism is forbidden and all unsupervised coursework items must be accompanied by a Coursework Accountability Statement in order to be assessed. Specific items may require submission through Turnitin on myeLearning. HOW TO STUDY FOR THIS COURSE Attendance is mandatory for lectures, tutorials and practicals. Full use should be made of the resources provided and students are strongly advised to become familiar with them and start utilising them from the first week. Students are encouraged to interact regularly with their lecturer on their projects, even outside of the assigned tutorial times to ensure satisfactory solution of any problems and to monitor progress. 216 Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 217 THE UNIVERSITY OF THE WEST INDIES, ST. AUGUSTINE FACULTY OF SCIENCE & TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE DOCUMENTATION Course code: BIOL3768 Course Title: Plant Diversity and Systematics Credits: 3 Level: 3 Semester: 2 Pre-requisites: BIOL1262 Living Organisms 1 or BIOL1261 Diversity of Organisms and either BIOL2XXX Physiology of Plants or BIOL2761 Plant Physiology COURSE DESCRIPTION: This course provides an overview of plant diversity and systematics and explores the origin and diversity of vascular land plants emphasizing flowering plants in the flora of Trinidad and Tobago. The course covers taxonomy (identification, nomenclature, and classification), diversity, morphology and evolution of vascular plant groups, as well as phylogenetics (phenetics, cladistics, morphology and molecules). Practicals focus on skills and activities necessary for indentifying vascular plants in Trinidad and Tobago and the tools necessary for the understanding of the study of systematics. The course would be taught using interactive lectures, tutorials and hands on practical sessions. Assessment would consist of a final written examination and in course, online and practical assignments. PURPOSE OF THE COURSE/RATIONALE: This is a core course for the Plant Biology option in the Department of Life Sciences and is an essential area in plant and, environmental biology. It also broadens the scope of studies in biology. INSTRUCTOR INFORMATION: Name of course coordinator: Judy Rouse-Miller Office address and phone: Room 322, Floor 3, Old wing, Natural Sciences Building Email address: [email protected] Office hours: Mondays 10-11am, Fridays 11 am -12 noon Preferred method of contact: Email Communication policy: Students should use their UWI email account for communication. 218 LETTER TO THE STUDENT Welcome to this course “Plant Diversity and Systematics” This course lays a foundation, improving your knowledge and skills for identifying plants; and then progresses to increase the range of plants you are able to identify. At the end of this course you will be able to look at plants with greater focus, critically observing the characteristics that differentiate a plant from others, and placing it into a group (family or genus) which exhibits those characteristics. This is a valuable skill for students interested in the environment and careers in ecology and the environment. CONTENT: Topics will include: Phylogenetics - phenetics, cladistics, morphology and molecules Classification, identification, nomenclature, emphasizing flowering plant families Diversity, morphology and evolution vascular plant groups: Lycophytes Pteridophytes Gymnosperms Early diverging angiosperm families Eudicots: Eurosids I evolution and morphology Eudicots: Asterids I morphology and evolution Petaloid monocots: morphology and evolution. Commelinoid monocots I morphology and evolution Extant ferns Incorporating phylogenies into conservation decisions The future of systematics GOALS/AIMS: This course aims to: Introduce students to the diversity and systematic of vascular plants with emphasis on flowering plants Expand a student's knowledge of the diversity and biology of living organisms by reference to vascular plants Link traditional and modern approaches in plant systematics GENERAL OBJECTIVES: The general objectives of this course are to: Provide course materials, activities and assessment using both face-to-face and online activities 219 Facilitate opportunities for students to engage with general concepts and specific examples to develop a comprehensive understanding of the plant diversity and systematic Guide students in critically analysing and exploring concepts in plant diversity and systematics using investigative hands-on approaches Facilitate discussion on the relationship between plants groups LEARNING OUTCOMES: At the end of this course students should be able to: Outline the methods used in plant systematics Evaluate the contribution of modern methods used in plant systematics Discuss key concepts in plant diversity Conduct a plant systematic investigation given the necessary tools Identify native plants from a range of natural environments using a range of techniques and resources, including the herbarium. Characterise plant species based on morphological characteristics Analyse systematic data in particular and scientific data in general Outlines the implications of systematic data for other biological disciplines COURSE ASSESSMENT: Assessment will be based on a student’s final mark from the coursework components below. Description Component % Final Grade Final exam 50% In-course tests 10 % 2-hour written exam to answer 2 out of 5 essay questions 1 test based on multiple choice, true/false, short response questions Performance in face-to-face and online discussions and activities Tutorials, online activities Plant Collection Systematics Project 10% 20% 10% Collect 20 plants representative of a range of local families: Systematics Project ASSIGNMENTS: 1 coursework test (10%): 50 minutes duration including multiple choice, true/false, short response questions Tutorials, online activities (10%): Continuous evaluation of participation and performance and contributions throughout the semester (discussions, wiki/ glossary, blogs) Plant Collection ( 20%): 25 plants representative of a range of families: pressed, dried and mounted 220 Systematics Project (10 %): Practical performance and reporting at 5 practical exercises during the semester EVALUATION: Feedback on the course will be obtained informally from students on an on-going basis by regular interactions and meetings among students, demonstrators, teaching assistants and the Course Coordinator in practicals and tutorials. Formal feedback will be via election of Class Representatives who sit on the Departmental Student-Staff Liaison Committee meetings held twice during the semester. Class reps will channel both concerns and commendations to the meeting as guided by the Department’s Standard Operating Procedures. Formal evaluation of the entire course will be accomplished via a UWI Course Evaluation questionnaire administered anonymously and confidentially at the end of the semester. All feedback will be considered on an on-going basis and corrective action or adjustments made or discussed with students promptly or incorporated the following year. TEACHING STRATEGIES: Contact hours :36 Lectures: 22 h Tutorials: 6 h Practicals: 8 h (4 x4 h = 16 h practicals) Lectures: Lectures will provide valuable synthesis and evaluation knowledge of diversity and systematics. Practicals: Fortnightly practicals will provide hands on experience for students to gain skills required for identification local flora. Participation and performance in the practicals will be assessed as well as reports based on the practical activities. Tutorials: Tutorials will cover course topics in an interactive format using collaborative learning techniques. myeLearning: The online teaching tool, myeLearning, will be used extensively during this course for communication among students and staff (email, discussions) official posting of important notices (coursework assessment notices, instructions, and incourse results) provision of course details, lecture notes, practical guides, tutorial briefings provision of recommended resource materials and links to resources on specific websites self-test quizzes, questionnaires, surveys interactive activities such as discussions, wikis, glossaries, blogs 221 RESOURCES: Most resources are available in myeLearning including Lecture presentations including learning objectives, summaries, recommended readings and links to papers, articles and websites with interactive resources and ‘General Resources’ provides direct links to general websites, articles and publications relevant to the whole course Tutorials may have specific supporting resources particularly on skills development Essential and Recommended texts are in the Reserve Section, UWI Main Library or via links in the course outline and myeLearning Essential texts Simpson, MG. 2006. Plant Systematics. Elsevier Inc, Burlington MA Harris, JG. and Harris-Woolf, M. Plant Identification Terminology: An Illustrated Glossary, ed. 2, Spring Lake Publishing Ennos and R and E. Sheffield 2000. Plant Life Blackwell Science, USA. Recommended text Carrington, S. 1998. Wild Plants of the Eastern Caribbean. London: MacMillan Education, Ltd. Comeau, P.L., Comeau, P. L. and Johnson, W. 2003. The palm book of Trinidad and Tobago including the Lesser Antilles. The International Palm Society. Quesnel, V. C. and Farrell, T.F. 2000. Native Trees of Trinidad and Tobago. Trinidad: The Trinidad and Tobago Field Naturalists’ Club. Woodland, Dennis W. 2009 Contempory Plant Systematics 4th edition Andrews University Press COURSE CALENDAR: WEEK 27. 28. PRACTICALS TUTORIALS LECTURES 74. Course Introduction 75. Phylogenetics I: What are phylogenetic trees? How to read them. 76. Phylogenetics I: cont’d – Choosing the best tree for your needs. 77. Phylogenetics II: Molecular characters Practical 1: Computer exercise -Learning to use phylogenetic software I Finding DNA sequences in GenBank, aligning the sequences, and making phylogenetic trees 222 Assignments Lab report due WEEK PRACTICALS TUTORIALS Tutorial 1Systematics project LECTURES Assignments 29. 78. Phylogenetics III: The power of modelling 79. Local flora and plant communities 30. 80. Lycophyte and Pteridophyte morphology and evolution 81. Gymnosperm morphology and evolution Practical 2: Lycophyte Pteridophyte and gymnosperm morphology and identification 31. 82. Classification and Nomenclature. 83. Origin of flowering plants: moncot/dicot evolution and morphology 84. Early diverging angiosperm families 85. Eudicots: Eurosids I evolution and morphology Tutorial 2 – Systematics project Practical 3: Tools in identifying angiosperm plant families Lab report due 86. Eudicots: Eurosids II evolution and morphology 87. Eudicots: Asterids I morphology and evolution 88. Eudicots: Asterids II morphology and evolution 89. Eudicots: Asteraceae morphology and evolution 90. Petaloid monocots: morphology and evolution. 91. Commelinoid monocots I morphology and evolution 92. Commelinoid monocots II: Poales 93. Extant ferns ancient or recent? 94. Incorporating phylogenies into conservation decisions 95. The future of systematics 96. Review 97. Review Tutorial 3 Systematics Project 32. 33. 34. 35. 36. 37. 38. Lab report due Practical 4: Field Trip – Plant Collections Tutorial 4 In Course Test Tutorial 5 Tutorial 6 223 Submit Plant Collection WEEK 39. PRACTICALS TUTORIALS LECTURES Assignments 98. Review ADDITIONAL INFORMATION: Students should access the Life Sciences Undergraduate Handbook for general guidelines for teaching, assessment, student conduct, essential skills, and support at http://sta.uwi.edu/fst/lifesciences/documents/handbook.pdf . Additionally, general information and regulations pertaining to the degree programme can be found in the Faculty Booklet available from http://sta.uwi.edu/resources/documents/facultybooklets/ScienceTechUndergrad.pdf . Students are reminded that tutorials and practicals are compulsory. An attendance register will be kept for these sessions. As a general rule, medicals or other excuses may only excuse a student’s presence at an assigned time. Students must complete the assigned tutorial/practical content. The student is responsible for liaising with the Course Coordinator or Teaching Assistant to ensure the assigned make-up is completed. Plagiarism is forbidden and all coursework items must be accompanied by a Coursework Accountability Statement in order to be assessed. Specific items must be submitted through Turnitin on myeLearning. Refer to ‘University Regulations on Plagiarism’ available from http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf HOW TO STUDY FOR THIS COURSE: Attendance is mandatory for lectures, tutorials and practicals. Prior preparation is necessary to be able to fully participate in activities and obtain the full value of the sessions. Use should be made of the resources provided and students should begin this from week. Myelearning should be checked on a regular and frequent basis. Students are expected to interact with Herbarium staff on their plant projects, to ensure prompt, satisfactory solution of any problems and to monitor progress. 224 Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 JRM: 24 January 2013 225 THE UNIVERSITY OF THE WEST INDIES ST AUGUSTINE FACULTY OF SCIENCE AND TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE OUTLINE Title: BIOL3769 – Plant Genetic Improvement Credits: 3 Level: Undergraduate Year 3 Semester: 1 Pre-requisites: BIOL2XXX Genetics II OR BIOL2162 Advanced Genetics OR AGCP2001 Principles of Crop Science and Production Anti-requisite: BIOL3763 Crop Improvement Offering Department: Department of Life Sciences, Faculty of Science and Technology, the University of the West Indies, St. Augustine. Course description: Plant Genetic Improvement is an elective for the Biology programme. The course deals with methods of plant breeding and the impact of biotechnology on plant breeding. The course addresses problems peculiar to the Caribbean so that students develop an appreciation of the problems, and the role of plant breeding in achieving a sustainable agricultural production system in the region. Students pursuing this course will acquire knowledge and skills to genetically manipulate autogamous, allogamous and vegetatively propagated plants using the plant breeding process towards developing sustainable agricultural production systems. Enrolment capacity: 50 students Course organization The course is divided into 10 major topics that progress naturally from each other. Students are introduced to the importance of plant breeding in fashioning variability that leads to the development of new, genetically superior varieties; the plant breeding process; the concept of evolution and domestication of plant species, man using plant breeding to design plants for his specific needs and desires; the factors that can and have contributed to genetic erosion (loss of germplasm) in several crop plants and the measures that can be taken to protect and conserve the variation present in domesticated plants; evolution of reproductive systems in plants; measures 226 used by plant breeders to augment plant genetic variability; manipulation of plant genetic variability; breeding methods in self-pollinated plants; breeding methods in cross-pollinated plants; and breeding methods in vegetatively propagated plants. Purpose of the course Plant Genetic Improvement (BIOL XXXX) has served as undergraduate course supporting M. Phil and PhD programmes in Life Sciences for many years. Students from the programme having completed graduate level programmes have found fruitful employment in the areas of teaching, research and agricultural policy in many countries in the Caribbean and worldwide. This course is earmarked to serve future MSc programmes in plant genetic resource management and utilization. Most countries in the Caribbean region have identified cultivation of low yielding and unadapted varieties of crop plants as amongst the most important deterrents to sustainable crop production. The high cost of production in the region is associated with lack of tolerance to biotic and abiotic stresses. With climate change looming, Plant Genetic Improvement is looked upon as the most viable option to create sustainable livelihoods. Much of the work for which the University of the West Indies and the Department of Life Sciences are known for comes from genetic improvement work done in a number of field and vegetable plant species (pigeon peas, bodi, blackeye peas, tomato, pepper), ornamental species (anthurium and orchids) and tree species (cocoa). Numerous high quality publications on Plant Genetic Improvement have also emanated from the Department of Life Sciences. Plant Genetic Improvement methods are being increasingly versatile by moving from phenotypic selection to molecular marker assisted breeding approaches, to genomics based selection approaches. Similarly traditional mutation breeding approaches have been replaced by targeted mutagenesis, to ‘tilling’ approaches. However, traditional breeding approaches still remain an essential part of stacking ‘transgenes’ and ‘cleaning up the genetic background’ in transgenics. Students without the basic skills of Plant Genetic Improvement may not be able to function in the new genomics driven world. Lecturer information Dr. Winston Elibox, Room 312, 3rd Floor Natural Science Building, Tel: 6622002 Ext. 83108; Email: [email protected] Office hours: Monday – Friday: 12:00 p.m. – 4:00 p.m. Communication Policy: I prefer communication via e-mail using your UWI email account. Letter to the students Dear Students, I wish you a warm welcome to Plant Genetic Improvement (BIOL3XXX). I am excited that you have decided to pursue this course which will provide you with the knowledge and skills of plant genetic improvement and the traditional and cutting edge tools used in this modern field. This is the primary advanced level course that generates most of the graduate research in the Department 227 of Life Sciences in the areas of Plant Breeding and Biotechnology. Furthermore, it’s the only course of its kind offered by the University; hence, you are part of the very prestigious group of students and future scholars. The course is myelearning supported and several resources including the course handbook and other suggested readings can be accessed at your convenience. Several thought provoking questions will be posted in the discussion forum of myelearning as we go through the course content and you are encouraged to participate. Please check your timetables and pay particular attention to lecture times, tutorials, assignment submission deadlines and incourse assessments. I wish you a fruitful semester and I look forward to working with all of you. Sincerely, Dr. Winston Elibox Lecturer Content Topic 1: Importance of plant breeding in plant improvement Role of plant genetic improvement in sustainable agriculture Breeding objectives to improve yield, adaptability and quality Topic 2: Plant breeding process Important elements of the plant breeding process Factors that determine the course of the plant breeding process to enhance economically important traits Topic 3: Evolution and domestication of plant species Changes that occurred with domestication of wild plants (crops) Factors that eventually led to differentiation in a crop species and in what form such variability presently exist Evolutionary history of some important crop plants Importance of centers of diversity of crop plants Topic 4: Genetic erosion and methods of conservation Factors that lead to genetic erosion The Green Revolution and its role in genetic erosion Effects of genetic erosion: inbreeding depression, bottleneck effect, loss of land races Curbing genetic erosion- crop germplasm conservation- in situ vs. genebanks Topic 5: Evolution of reproductive systems in plants Reproductive systems in plants- Vegetative vs. Sexual reproduction Self-pollinators vs. Cross-pollinators Apomixis and its use in Plant Breeding Male sterility and its use in Plant Breeding Incompatibility systems in plants 228 Topic 6: Creating genetic variability in crop plants Mutations Somaclonal variations (mutations in tissue culture) Hybridization: intraspecific, interspecific Polyploidy/ Chromosome manipulation methods Genetic Engineering Topic 7: Manipulation of genetic variability Selection: Natural selection vs. Artificial selection Principles of Selection, factors affecting genetic advance, heritability estimates, heritability and response to selection Molecular markers: Marker assisted selection; Advantages of marker assisted selection Topic 8: Breeding methods in self-pollinated crop plants Pedigree method Isolines, multilines and composites Bulk method Single seed descent Back cross method Double haploid breeding Topic 9: Breeding methods in cross-pollinated crop plants Mass selection Ear-to-row selection Progeny selection and line breeding Recurrent selection methods Hybrid breeding- single cross and double cross varieties Synthetic varieties Topic 10: Breeding methods in vegetatively propagated crops Clonal breeding- macropropagation vs. micropropagation Goals At the end of this course students should have: Acquired skills to genetically manipulate autogamous, allogamous and vegetatively propagated crop plants using the plant breeding process Understood the importance of the process in a sustainable agricultural production system General Objectives The course aims at providing students with the knowledge and skills required for Plant Genetic Improvement through lectures, discussions, a field trip and tutorials. The course is broken up into 229 10 topics in a sequential (logical) order and class topics are reinforced and extended during assignments, the field trip and class discussions. To assess learning, three incourse exams (weeks 4, 8 and 12) and three assignments (week 2, 5, 9) will be given and graded. For the assignments, students will be split into smaller groups and given two weeks to prepare the reports for each assignment. However, each student must submit an individual report for each exercise. Furthermore, students will get to see an actual case of plant genetic improvement in practice through a field exercise (week 11), and should be able to formulate breeding strategies to genetically improve any crop of interest. Students must submit a report on the field trip for grading. The course is myelearning supported and your manual contains specific course objectives as well as lecture outlines and more. Students’ answers to the course questions posted on myelearning will also be used to assess learning. Learning Outcomes: At the end of this course a student should be able to: Topic 1- Importance of plant breeding Describe the role of plant genetic improvement in attaining a sustainable agricultural production system that will meet the increasing demand for food, clothing and other industrial products derived from crop plants in the 21st century. Discuss the role of plant genetic improvement in the management of pests and diseases; specifically outline breeding objectives that would lead to increases in productivity and profitability, adaptability and quality. explain 'ideotype breeding'. Discuss the statement 'Good seed can substitute for inputs in crop management'. Topic 2 - The plant breeding process Define the important elements in the plant breeding process. Discuss the factors that determine genetic variability of a crop plant species and how genetic variability can be further enhanced in crop genetic improvement programmes. Discuss the factors that determine the course of plant breeding such as breeding system, heritability of trait, type of crop plant- tree/annual/ornamental, end variety desired and economic considerations. Topic 3 - Crop domestication and crop evolution Define the term domestication. Discuss the major changes that took place as a result of domestication. 230 Explain what factors led to differentiation in a crop plant species and in what form such variability presently exist. Topic 4 - Genetic erosion and methods of conservation Define the term 'genetic erosion' and describe the processes that have contributed to it. Discuss methods that can be used to minimize genetic erosion and justify the need for these methods in conservation programmes. Discuss the concept of center of diversity (Vavilov vs. Harlan) and its importance to a crop species. Discuss the roles of a gene bank and the major players in the conservation process. Differentiate between primary, secondary and tertiary germplasm and their importance in conservation efforts. Discuss the evolutionary history of some important food crop species- the grasses (corn, wheat, rye, barley, sugar cane), banana. Topic 5 - Evolution of reproductive systems in crop plants Asexual reproduction (vegetation) vs. sexual reproduction. Show that the objectives in breeding for any crop species, method of breeding and the types of varieties produced depend on the 'breeding system of that particular species. Sexual reproduction Distinguish between autogamous, allogamous and vegetatively propagated crops. Describe the mechanisms in crops that enforce/ promote autogamy and those that enforce/promote allogamy. Distinguish between sterility and incompatibility systems. Self-incompatibility Define self-incompatibility. Classify the various types of self- incompatibility. Give a comparative account on the types of self-incompatibility. Describe methods of overcoming the self –incompatibility systems. Male sterility Define male sterility. Explain the various types of male sterility. Describe the use of male sterility systems in hybrid seed production. describe how the various male-sterile lines are maintained. Apomixis Define apomixis. 231 Describe the types of apomixis producing diploids and those producing haploids and among those producing haploids the types leading to maternal, paternal or mixed haploids. Describe how the apomictic processes in some important crop plants can be manipulated in breeding. Manipulating breeding systems for man’s benefit Describe how plant-breeding systems can be manipulated to the advantage of the breeder based on genetic understanding of breeding systems. Topic 6- Creating genetic variability Outline methods used to generate variability in breeding programmes. Mutation breeding Explain the role of mutations in the evolution of crops, since domestication, with examples; should be able to discuss the various schools of thoughts for and against mutation breeding as a source of variability in breeding programmes. Discuss methods that are used in the induction of mutations - physical vs. chemicals; haplophase vs. diplophase; seeds vs. whole plants or vegetative parts etc. Discuss the advantages and disadvantages of mutation breeding programmes in plant genetic improvement. discuss the role mutation breeding programmes have played in plant genetic improvement in the past Discuss the use of mutation breeding in forward genetics, targeted mutagenesis and tilling. Somaclonal variations in plant breeding Define somaclonal variation and list factors that predispose somaclonal variation. Describe the cellular mechanisms (both genetic and epigenetic) that result in somaclonal variation. Discuss the role that somaclonal variants have played in the past in selected crop plants. Polyploidy in plant breeding Describe the role of ploidy in the evolution of crop plants. Discuss the factors that have led to the success of autopolyploids and allopolyploids. Explain the difficulties that arise when breeding higher ploidies. Discuss the role autopolyploids have played in plant genetic improvement in the past. Hybridization: intraspecific hybridization vs. interspecific hybridization Intraspecific hybridization in plant breeding Discuss the factors that contribute to variation in the F2 generation. Show the influence of parental choice and mating designs on variability. 232 Show that plant breeding is a numbers game as the probability of finding a progeny plant with superior economic traits increases with greater numbers of diverse crosses. Interspecific hybridization in plant breeding Describe the barriers to interspecific hybridization. Differentiate between different crossability barriers: prezygotic vs. postzygotic; hybrid sterility vs. genetic instability. Discuss the various methods used in plant genetic improvement to exploit interspecific hybrids with examples viz. vegetative propagation of F1 hybrids; allopolyploids; introgression; chromosome manipulation. Describe chromosome manipulation methods used in plant breeding demonstrate diagrammatically how chromosome addition lines and substitution lines are produced. Genetic engineering in plant breeding Discuss the importance of genetic engineering as a method of plant genetic improvement Describe the impact genetic engineering has had on plant genetic improvement. Topic 7- Manipulation of genetic variability Principles of selection Define selection; define the role of selection as an evolutionary force. Distinguish between stabilizing selection, directional selection and disruptive selection. Explain natural selection vs. artificial selection . Describe the response of various traits to selection. Explain the factors that determine response to selection (genetic gain). Describe the factors that affect selection intensity (cost, no. of characters; variability for future selection). Describe how a genetically variable population is created in the start of a breeding programme and how variability is maintained throughout the breeding programme to ensure continuous response to selection. Define heritability and be able to list problems encountered with phenotypic selection when heritability is low. Distinguish between heritability in the broad sense and narrow sense. Describe the methods used in the determination of heritability estimates and be able to work problems in the estimation of it. Explain how breeders deal with characteristics with low heritability. outline the following: reduce environmental influences; proper experimental designs; grid mass selection; late generation selection; progeny testing; indirect selection and marker assisted selection. discuss the rationale for late generation selection. 233 describe various methods of indirect selection methods used - selection of component traits; selection of an ideotype; marker assisted selection. Methods of selection Distinguish between tandem selection methods and simultaneous selection methods. Discuss the merits and demerits of independent culling and index selection. Self-pollinated crop plants (autogamous crops) pedigree selection bulk selection Cross-pollinated crop plants (allogamous crops) Define mass selection. Discuss the advantages and disadvantages of mass selection. Define progeny selection. Discuss the advantages and disadvantages of progeny selection. Define ear-to-row selection in corn. Discuss the advantages and disadvantages of ear-to-row selection. Define single plant / line selection. Discuss the advantages and disadvantages of single plant/ line selection. Define recurrent selection. Discuss the advantages and disadvantages of recurrent selection. Marker assisted selection for characteristics with low heritability Discuss the reasons for poor responses to selection for characteristics with low heritability, genetic linkage. Explain the rationale for marker assisted selection. Describe the use of morphological, biochemical and molecular markers that are linked to other important traits with low heritability. Describe how RFLP markers are generated. Explain the role of RFLP markers in plant genetic improvement. Explain the merits and demerits of using RAPD markers as opposed to RFLP markers. Topic 8 - Breeding methods in autogamous crop plants Outline the objectives in breeding self-pollinated crop plants. Pedigree breeding leads to pureline varieties outline the objectives of pedigree breeding. describe the steps in pedigree breeding. 234 discuss the advantages and disadvantages of pedigree breeding. Bulk population breeding method outline the objectives of bulk breeding. describe the steps in bulk breeding. discuss the advantages and disadvantages of bulk breeding. Single seed descent breeding method outline the objectives of single seed descent breeding method. describe the single seed descent method. discuss the advantages and disadvantages of the single seed descent method. Haploid breeding outline the objectives of haploid breeding. describe methods for creating haploids. discuss the advantages and disadvantages of haploid breeding. Double haploid breeding: describe methods for creating double haploids. discuss the advantages and disadvantages of double haploid breeding. Backcross breeding outline the objectives of backcross breeding. describe the backcross breeding method. discuss the advantages and disadvantages of backcross breeding. distinguish between purelines, isolines, multilines, composite varieties, bulk varieties. Topic 9 - Objectives of breeding in cross pollinated (allogamous) crop plants Breeding methods in allogamous crop plants Population improvement method of breeding outline the objectives of population improvement method of breeding. discuss the steps involved in the population improvement method of breeding. discuss that the population improvement method of breeding leads to the production of open-pollinated varieties (OPVs). Hybrid breeding in allogamous crop plants outline the objectives of hybrid breeding in allogamous crop plants. describe the steps involved in hybrid breeding. discuss the advantages and disadvantages of hybrid breeding in allogamous crop plants. discuss the factors that lead to hybrid vigor and how this is exploited in hybrid breeding. 235 describe how inbred lines, single crosses and double crosses are created and outline the reasons for creating each type of hybrid. Breeding of synthetic varieties define synthetic varieties. outline the objectives of breeding synthetic varieties. describe the steps involved in creating synthetic varieties. discuss the advantages and disadvantages of synthetic varieties. Discuss the advantages and disadvantages of hybrid varieties vs. open pollinated varieties (OPVs). Distinguish between population improvement methods and population enhancement methods. Topic 10 - Breeding methods in vegetatively propagated crop plants Clonal breeding describe clonal breeding and its advantages and disadvantages. describe the use of macropropagation and micropropagation techniques in clonal breeding. Assignments/ field trips Students to research the Green revolution (1940s to late 1970s) and the present day gene revolution (5%). differentiate between the green revolution which revolutionized crop genetic improvement and gene revolution. outline the successes and failures of the green revolution and gene revolution. explain why the green revolution was so wide spread and why the gene revolution has not made significant impact in developing and underdeveloped countries. Group exercise - Evolution and domestication of selected crop plant species (5%). Students will be placed into groups of five and asked to research the evolution and domestication of one crop species per group. Each group will make a 15 minute power point presentation to the class. At the end of this exercise, students will be able to: present information about the crop as it pertains to the uses of the crop, its evolution, center of origin and diversity, time since domestication, the movement of the crop around the world, the changes that have been brought about by domestication, the different varieties present in cultivation today. show that the greater the length of time that a crop has been domesticated, the greater its diversity, adaptability and uses by man. 236 Field trip to the International Cocoa Genebank Trinidad (5%). Students will be given a guided tour of the International Cocoa Genebank at Centeno, Trinidad to see how a genebank is managed. At the end of the field trip, students should be able to list the problems associated with cocoa germplasm conservation (lack of adequate skilled labour, financial constraints, lack of adequate equipment, problems with mislabeling of clones, managing diseases in the collection, problems with providing planting material to stake holders upon request, duplications in the gene bank); list the opportunities for having the international genebank (all the genetic variability in cocoa is available for use; genetic fingerprinting to determine unique clones and eliminate duplications; population enhancement methods; possibility of obtaining funding for research projects; hybridization efforts create exciting new variability, new textures and flavours). Worksheet: calculation of heritability in the broad and narrow sense and their use in breeding; realized heritability vs. heritability estimates (5%). At the end of this worksheet, students will be able to: Calculate heritability estimates from data obtained from different experimental designs. Use different statistical methods to calculate heritability estimates. Calculate true heritability- realized heritability. Show the importance of obtaining heritability estimates in breeding programmes in order to determine how much improvement can be made per breeding cycle. Course assessment In-course Incourse examinations will include multiple choice questions, structural and short answer type questions. These exams will assess knowledge, comprehension and application of course content. The assignments and field trip will allow students the opportunity to write reports using the scientific method and as well reinforce and extend what is learned during class time as well as allow students to analyze real life situations in Plant Genetic Improvement. (See the course calendar for the assessment schedule). Incourse exam-1 Incourse exam-2 Incourse exam-3 Field trip/ worksheet/ assignment reports 10% 10% 10% 20% 237 Total incourse marks 50% The final examination comprises multiple choice questions, short answer questions with four to five parts, structured questions and essay type questions. The final examination will also test students’ ability to recall, comprehend, apply and analyze course content in a critical and logical manner. Students are required to answer ALL questions. Overall assessment Incourse Final Theory Exam (2 hours) 50% 50% Evaluation Two student representatives will be elected from the class. Students’ feedback will be obtained officially from the staff-student liaison meetings held twice per semester. However, students are encouraged to give their feedback on the course via myelearning at any time. Comments will be assessed critically by the instructor, course coordinator and first examiner and weaknesses will be addressed to improve on the course in the future. Teaching Strategies The course consists of 32 lectures, ONE field trip, THREE assignments and THREE tutorials. The course is myelearning supported and your manual contains specific course objectives as well as lecture and lab outlines, past exam papers and model answers. Resources Text Category A Simmonds, N.W. 1979. Principles of Crop Improvement. Longman Inc, NY, USA. Simmonds, N, W., and Smartt, J. 1999. Principles of Crop Improvement, 2nd Ed. Blackwell Science, Oxford, UK. Category B Allard, R.W. 1999. Principles of Plant Breeding 2nd ed. John Wiley and Sons, NY, USA. Chrispeels, M. J., and Sadava, D. E. 2003. Plant, Genes and Crop Biotechnology. Jones & Barlett Learning, MA, USA. 238 Category C Poleman, J.M. 1979. Breeding field crops. AVI Publishing Company, Connecticut, U.S.A. Fehr, W.R. 1990. Principles of cultivar development Vol1 &Vol11. Macmillan Publishing Company, NY, USA. Stalker, H.T. and Murphy, J.P. 1992. Plant Breeding in the 1990s. CAB International, Wallingford, UK. Readings http://kkvinod.webs.com/share/cms.pdf http://theagricos.com/plant-breeding/self-incompatibility/sporophytic-selfincompatibility-ssi/ Self-incompatibility Deborah Charlesworth F1000 Biology Reports 2010, 2:68 (doi:10.3410/B2-68) http://f1000.com/reports/b/2/68/pdf Course Calendar Week Topic 1 Introduction- overview Objectives of plant genetic improvement Plant breeding process/ Structure and importance of genetic diversity 2 Evolution and domestication of crop plant species Genetic erosion and conservation of genetic resources Green revolution and its effects on crop plant diversity/ green evolution assignment given 3 Breeding Systems in Plants/ review of flower structure Mechanisms for enforcing breeding systems Self-incompatibility/ self-sterility 4 Male sterility Apomixis INCOURSE EXAMINATION-1 Manipulating breeding systems for man’s benefit/ collect assignment on 5 green revolution Mutation breeding-1 (conventional methods) Mutation breeding-2 (targeted mutagenesis/ tilling)/ Students are grouped and given assignment on the evolution and domestication of selected crop plant species 6 Exploitation of somaclonal variations 239 7 8 9 10 11 12 13 Induction and exploitation of polyploids Exploitation of intraspecific hybridization Crossability barriers to interspecific hybridization Exploitation of interspecific hybridization/ Collect assignment on evolution and domestication of selected crop plant species Bioengineering (genetic engineering) – GMO’s INCOURSE EXAMINATION-2 Principles of selection / Students are asked to read up on the functions of the International Cocoa Genebank in Trinidad. Types of selection/ Marker assisted selection Heritability and response to selection-1 Heritability and response to selection-2 Autogamous crop plants- Pedigree method of breeding/ Students are provided with worksheet on calculating heritability estimates for various traits Autogamous crop plants- bulk population method of breeding Autogamous crop plants- single seed descent method of breeding Autogamous crop plants- haploid method of breeding/ Collection of assignment on calculating heritability estimates Autogamous crop plants- backcross method of breeding Allogamous crop plants- Population improvement and enhancement methods Field trip to International Cocoa Genebank/ discussions Allogamous crop plants- Hybrid breeding/ synthetic varieties/ Collection of report pertaining to the fieldtrip to the International Cocoa Genebank Clonal breeding in vegetative crop plants/ INCOURSE EXAMINATION -3 Tutorial Tutorial Tutorial Additional information Attendance Students should attend all sessions in the course. Attendance for the field trip is mandatory. Any student who misses the field trip or an incourse exam is advised to consult immediately in person or by email with the course instructor regarding their make-up options. Absence must be accompanied by a written excuse or medical submitted to the Main office, Life Sciences within 7 days of the missed session. Any student who was inexcusably absent from the field trip or incourse exam, or who does not submit an assignment report will receive 0% for that exercise. 240 How to study for this course Students are encouraged to work together in small cohesive groups as much as possible to go through the course content. As we go through the various topics, students should attempt to answer all the sample questions placed on myelearning and discuss the answers amongst themselves. All comments, questions and concerns provided on a particular topic will be addressed during class time. Your course textbook on myelearning contains all the topics to be taught and its content is aligned similarly as the lectures; please read the textbook. Each new topic covered has its basis in previous topics. Link the parts of the course to see the big picture. Use the feedback for your reports and incourse examinations as a guide to answering questions and writing reports properly. There are several pass paper questions in the library and students are encouraged to attempt these questions. Finally, students are encouraged to design sample breeding programmes for various types of crops with different floral biology and for different uses. Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 241 THE UNIVERSITY OF THE WEST INDIES, ST. AUGUSTINE FACULTY OF SCIENCE & TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE DOCUMENTATION TITLE: BIOL3770- PLANT PATHOGENS CREDITS: 3 LEVEL: Undergraduate-Year III SEMESTER: I PRE-REQUISITES: BIOL2XXX Fundamentals of Microbiology ANTI-REQUISITE: BIOL3767 Biology of Plant Pathogens COURSE DESCRIPTION: This course introduces the learner to the field of plant pathology and provides basic information on the biology and epidemiology and disease cycle of important plant pathogens affecting plants of this region. This course provides comprehensive knowledge about plant pathogens and their interactions with the host. Topics include: Biology of plant pathogens; Classification of plant pathogens; their cellular organization, structure; Examples of pathogens; Pathogen-life cycles, disease cycle; Symptomology; epidemiology, spread, survival; Host-pathogen interactions, mechanism of infection, physiological and biochemical processes of infection; Host resistance and defense mechanisms; Principles of disease management; Molecular-based pathogen detection and disease diagnosis. This course will be taught through lectures/tutorials, lab sessions, field trips. Students are expected to complete a group project. COURSE RATIONALE: Plant pathology has grown into an important field of plant science and biology. Damage and yield loss of crops by plant diseases may result in hunger and starvation, especially in developing and underdeveloped countries. The estimated annual global loss ranges betweens of 30 to 50 percent for major crops. In some years, losses are much greater, producing catastrophic results for those who depend on the crop for food. Major disease outbreaks among food crops have led to famines and mass migrations throughout history. With the projected increase in population in the coming decades it becomes increasingly challenging to improve the yields beyond thresholds and one the constraints is management of plant diseases. Hence imparting knowledge about plant pathogens and diseases is important particularly to students majoring biology or agriculture. Knowledge about microorganisms is a must and an important component of any basic or applied life sciences program. This course is precisely compatible with the present and future missions of the University. UWI plays a major role in promoting and conducting basic and applied research towards improving the agricultural productivity of the Caribbean region towards self-sufficiency before the end of first quarter of this century. It would be made possible only by imparting knowledge and by training students on understanding the basic issues related to crop protection, 242 disease diagnosis and pathogen identification. In this context this course introduces the learner to the field of plant pathology and provides opportunities for further learning and for research. This course serves as a necessary component of microbiology concentration for students majoring B.Sc. Biology. INSTRUCTOR’S INFORMATION Dr. Jayaraj Jayaraman Room 221, Natural Sciences Building, UWI- Life Sciences Phone: 868-662-2002 Extn. 3092; Fax: 868-663-5241 http://sta.uwi.edu/fsa/lifesciences/jjayaraman.asp Email: Jayaraj [email protected] Preferred contact – By Email LETTER TO THE STUDENT Welcome to the course, “Plant Pathogens”. This course which provides an opportunity to learn more about the basics of biology of plant pathogenic organisms and their interactions with the host plants in detail. This course comprises both theory (2 hours/week) and practical sessions (6). The practical classes impart skills on isolation and inoculation of plant pathogenic microbes and molecular-based detection of pathogens in diseased plants and produce. Prior knowledge on microbes, as listed in the pre-requisites is essential for better understanding of the contents of this course. Students will be able to access the course material and work on the exercises through online through myelearning. Course materials are located and constantly updated in myeLearning. CONTENT The course covers, Biology of plant pathogens including, plant pathogens-types, their cellular organization, life cycle, propagation, primary and secondary spread and survival. Plant-microbe interaction including, pathogenic and non-pathogenic plant interactions, Mechanism of pathogenesis of plant pathogens. Symptomology, physiology of parasitism, plant defense mechanisms, Pathogen variability. Host-resistance, sources and inheritance of resistance, R-genes, resistance mechanisms, genetics and genomics of resistance. Relevance of knowledge on pathogens for developing integrated disease management methods. identification of plant diseases, isolation and identification of plant pathogens, inoculation methods and principles of molecular- based detection techniques. GOALS/AIMS The goals and aims of this course are to, Offer required knowledge on the nature and life cycle of plant pathogenic microorganisms. 243 Impart practical skills on identification of pathogens, diagnosis of disease symptoms, isolation of pathogens by pure culture methods, inoculation techniques and introduction to molecular-based diagnosis. Provide necessary knowledge and information on the phenomenon of pathogen infection and their physiological and biochemical mechanisms, host-pathogen interactions, host responses, host resistance mechanisms including R and avr genes. Teach the principles and develop competence for identification of plant pathogens and disease diagnostic techniques. Enhance the understanding of current scientific research advancements in plant pathology. . LEARNING OUTCOMES At the end of this course students will be able to, Investigate and identify a plant disease based on classical and molecular approaches. Ascertain the causal agents (biotic and abiotic). Distinguish the symptoms and causal pathogens. Summarize the principles involved in molecular-based detection techniques and demonstrate their utility in some practical situations. Illustrate the disease cycles of pathogens, mode of spread and survival. Discuss the mechanisms of infection, physiology of infected plants and host-pathogen interaction, virulence and pathogenicity. Analyze and summarize the mechanisms of resistance and justify the implications of resistance as a means of disease management. Illustrate the principles of disease management strategies and relate with the knowledge on plant pathogens. COURSE ASSESSMENT 50% Course work 50% Final examination Course assessment components % split up details Incourse tests (2x9%) 18% Assignment (4%) &/Online activities (6%) 10% Group Project 15% Lab quiz (2x2%) and lab observation report (3%) 7% Final examination 50% 244 EVALUATION The elected Class Representative and/or Deputy will attend meetings with the course teacher(s) organized at every 4th week, and present feedback from the students attending that course or stream. This feedback is normally provided both orally and in written form for transmission to the lecturer. Apart from that the representatives will be attending the Liaison Committee meeting, and give their feedbacks to the committee. Students may comment on any aspect of the course or facilities. Students will be encouraged to submit their feedback (oral/written) during tutorials directly to the course teacher and appropriate actions will be taken by the teacher then and there. Results of in-course tests, quiz and other course assignments will be analyzed and presented in the class. This will help students to check their progress constantly and also helps the instructor to identify the weak areas and thereby could alert and advise students individually to alter their approach of study and completing the work. The final reflective feedback and comments about the entire course and teaching will be collected on the last day of the course. This will be saved for analysis and utilized as a base for improvement for the next offering in the following year. TEACHING STRATEGIES* Contact hours: 36 h Lectures &Tutorials: 24 h Practical work: 12 h The instruction for this course would comprise of 2 Lectures with inbuilt tutorials (40 min lecture+10min tutorial) per week and 6 lab sessions. Additionally, instructional videos, tutorials and practical labs would be utilized to support the teaching. Formal lectures and tutorials will provide introduction to specific topics and allow for discussion in an interactive setting. Case studies, journal papers and data will be considered in small group and class settings. Course materials and readings will be available online in advance so that discussion and critiquing can take place within the assigned class time. This course is also my e-learning supported, and many other resources are readily available for the students. There will be a group project which is one of the components that fulfills the 15% of the marks. The project will be decided by the course teacher and it will be executed by the students in groups. The course teacher will supervise and help students in accomplishing the project. The project normally involves preparation of disease specimens, status report, pathogen isolation and preparation of cultures and microscopic slides of pathogens. The practical project may be carried out during the lab hours. RESOURCES Recommended reading: Agrios, G.N. 2005. Principles of plant pathology, Academic Press; 5 edition. Nicholas, R., Windham, M.K., Windham, A., 2004. Plant pathology: Concepts and laboratory exercises, CRC Press. Mehrotra, R.S., Aggarwal, A. 2003. Plant pathology, Tata McGraw Hill. Narayanasamy, P. 2001, Plant Pathogen-Detection & Disease Diagnosis II edn., CRC Press 245 Narayanasamy, P. 2008, Molecular Biology in Plant Pathogenesis and Disease Management: Vol. 1&2, Disease development, Springer. Rangaswami, G., Mahadevan, A. 2006. Diseases of Crop Plants in India, Prentise-Hall of India. Sharma, P.D. 2004. Plant Pathology, I edition, Rastogi Publications, India. Journals: Phytopathology (American Phytopathological Society) Plant Pathology (Wiley-Blackwell) Canadian Journal of Plant Pathology (Taylor & Francis) Annual Review of Phytopathology (Annual Reviews, Inc.) Other resources: The ourse tea her ill upload i my e-lear i g , periodi all at the egi i g a d duri g the ourse , important research articles, review papers, book chapters and other related study materials. Students are expected to read them as these were considered and included as portions of the syllabus and covered during examinations. Course Calendar Week Lecture &Tutorial Lab Wk1 Classification of plant pathogens and plant diseases Symptomology Wk1 Field Trip (within Trinidad) Viral pathogens (classification, life cycle, spread) Wk2 Viral pathogens (replication, movement) Wk2 Wk3 Viral pathogens – examples (Activity-1) MLO s a d ‘LOs a d X le -borne bacteria 246 Isolation of plant pathogens, Quiz1 Wk3 MLO s a d ‘LOs and Xylem-borne bacteria examples Wk4 Bacterial pathogens (classification., life cycle, spread) Wk4 Ba terial pathoge s, e a ples… Incourse I Wk5 Fungal pathogens (classifn., life cycle, spread) Wk5 Fu gal pathoge s, e a ples… A ti it -2) Wk6 Fu gal pathoge s, e a ples… Wk6 Nematodes as vectors, nematode-pathogen complexes Identification of Plant pathogens Inoculation of plant pathogens, Quiz 2 Wk7 Phanerogamic parasites Wk7 Disease cycles (Activity-3) Wk8 Survival of plant pathogens Wk8 Spread of pathogens Wk9 Factors affecting infection Wk9 Mechanism of infection (Activity-4) Wk10 Epidemiology Wk10 Host responses and resistance mechanisms Wk 11 Genetic resistance Molecular Plant Diagnosis Visit to Electron Microscope facility at Mount Hope, In-course 2 247 Wk11 Induced resistance Presentation and submission of Project report & Lab Obs. Report Wk12 Plant disease diagnosis Wk12 Principles of Plant disease management Wk13 Review Tutorial Wk13 Review Tutorial ADDITIONAL INFORMATION: Refer to the Life Sciences Undergraduate Handbook for general guidelines for teaching, assessment, student conduct, essential skills, and support, General safety guidelines in Lab and General Safety Practices at http://sta.uwi.edu/fsa/lifesciences/documents/handbook.pdf . Students are reminded that they must attend a minimum of 75% of the practical sessions and tutorials. Failure to do so will result in debarment from the final examination. As a general principle, medicals or other excuses may only excuse a student’s presence at an assigned time. Students must still complete the assigned work (make-up lab report or make-up test) in order to obtain the marks for that item of coursework. The student is responsible for liaising with the Course Coordinator or Teaching Assistants to ensure the assigned make-up is completed. Students are hereby informed that plagiarism is forbidden and all unsupervised coursework items must be accompanied by a Coursework Accountability Statement in order to be assessed. Specific items may require submission through Turnitin on myeLearning. Refer to ‘University Regulations on Plagiarism’ at http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf HOW TO STUDY FOR THIS COURSE: Attendance is mandatory for lectures, tutorials and practicals. Prior preparation is strongly advised to able to fully participate in activities and obtain the full value of the sessions. Thorough use should be made of the resources provided and students are strongly advised to become familiar with them and start utilising them from the first week. Regular updates on course progress and materials are also highly recommended and you should be checking into myelearning on a frequent regular basis to review materials, assignments and activities. Students are encouraged to interact regularly with staff, even outside of the assigned tutorial times to ensure prompt, satisfactory solution of any problems and to monitor progress. 248 Good group management is essential and requires good communication and interpersonal skills. Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 THE UNIVERSITY OF THE WEST INDIES, ST. AUGUSTINE FACULTY OF SCIENCE & TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE OUTLINE COURSE CODE: COURSE TITLE: SEMESTER: LEVEL: NO OF CREDITS: PRE-REQUISITES: BIOL 3771 Environmental Plant Physiology 2 3 3 BIOL2XXX Physiology of Plants or BIOL2761 Plant Physiology ANTI-REQUISITE: BIOL3766 Plant Ecophysiology COURSE DESCRIPTION The course consists of two parts with an in-course exam following each part. The first part introduces the essential concepts of Environmental Plant Physiology and looks in detail at three 249 important abiotic factors: light, water and temperature. Each topic is covered by lectures and supported by online materials and by recommended reading. The Practicals complement the lecture topics and provide an opportunity to assess the students’ comprehension of each topic, while at the same time providing the students with valuable practical skills in the life sciences. The second part examines the application of these concepts through a series of case studies looking at different habitats and applied scenarios. In addition to case studies developed by the instructor, students work in structured groups to develop and present their own case studies that explore the role of plant research in meeting the challenge of global climate change. Assessment will be based on a group presentation, practical reports, two in course exams and a final theory exam. PURPOSE OF THE COURSE/COURSE RATIONALE The course is designed to build on the concepts gained in the prerequisite course and illustrate how basic science can be applied in research and development activities. Environmental Plant Physiology complements other courses offered in the life science department, focusing on the abiotic factors that influence plant growth and survival. This course will equip students with the knowledge of how plants can be used to conserve land, restore ecosystem services, and provide sustainable food and energy. Within the Life Sciences department it will be a core module for the minor in Plant Biology and an elective for minors in Biotechnology, and Ecology &environmental Biology, as well as for the Biology major. INSTRUCTOR’S INFORMATION Name of course coordinator: Office address and phone: Email address: Office hours: Preferred method of contact: Communication policy: Aidan D. Farrell 220, Zoology Office, Natural Sciences Building, New Wing Natural Sciences Building [email protected] 9:00 - 5:00 daily (email for appointment) Email Students should use their UWI email account for communication and can expect a response within 48 hours COURSE CONTENT Environmental Plant Physiology focuses on the interaction between plants and their environment, exploring the diverse ways in which plants adapt to and manipulate their surroundings. Environmental Plant Physiology provides a framework for many applications of plant science in the management of natural and manmade ecosystems. The course begins with an overview of microclimate, community interactions and of the many factors that limit plant growth and survival. Subsequent lectures focus on three key abiotic factors and the way plants have adapted to them. The course includes a case study component (see below: Case Studies and Applications) that illustrates the importance of current research in supporting a range of sustainable ecosystems, from rainforest stands to cultivated fields. In addition to case studies developed by the instructor, students will have the opportunity to develop their own case studies that explore the role of plant research in meeting the challenge of global climate change. Essential Concepts in Environmental Plant Physiology Microclimate and community interactions 250 Water as an environmental factor Water stress (drought) Light as an environmental factor Light stress (shade and excess) Temperature as an environmental factor Temperature stress (chilling and heat) Case Studies and Applications Habitat case study (mangrove) Habitat case study (agricultural) Environmental Plant Physiology in action (habitat restoration) Environmental Plant Physiology in action (crop production) The challenge of climate change GOALS/AIMS This course aims to: Review the essential concepts in Environmental Plant Physiology Provide students with the knowledge needed to understand the role of physiological research in managing and restoring ecosystems Enable students to develop practical skills in assessing the performance of plants in various natural and manmade ecosystems Train students to research and present scientific arguments on relevant topical issues GENERAL OBJECTIVES To provide explanations of the key concepts in Environmental Plant Physiology To demonstrate a range of methods used in Environmental Plant Physiology To describe the application of plant research in the assessment and management of different ecosystems To utilise group work and case studies to facilitate the students in applying their understanding to different systems LEARNING OUTCOMES The lecture and practical schedule is designed so that at the end of this course students should be able to: Evaluate the suitability of particular environments for a given plant or habitat type Appraise the performance of living plants in terms of: Water uptake and utilisation Primary productivity and gas exchange Stress tolerance and response Formulate, research and present scientific arguments on topical issues, such as: Ecosystem services Habitat restoration Sustainable agriculture 251 ASSIGNMENT Five practical reports will serve to train the students in designing and reporting on experiments. They will also help to assess the extent to which material presented in the lectures is retained by the student. The course includes a case study component (Case Studies and Applications) that illustrates the importance of current research in supporting a range of sustainable ecosystems. In addition to case studies developed by the instructor, students will work in structured groups to develop their own case studies that explore the role of plant research in meeting the challenge of global climate change. The group presentations form a core part of the teaching strategy. They will allow the students to interact and to assess their own knowledge relative to that of their colleagues. As well as assessing their knowledge of Environmental Plant Physiology, this assignment will be used to evaluate the student’s ability to understand and communicate scientific arguments. COURSE ASSESSMENT Students are required to achieve a grade of 40% overall, irrespective of their performance in individual components. Component % Final Grade Final exam 50% In-course test 1 10% In-course test 2 10% Practicals 10% Case study 20% Description 2-hour written exam with 20 multiple choice questions, and a choice of 2 out of 4 essay questions Essential Concepts in Environmental Plant Physiology: multiple choice and short structured questions Case Studies and Applications: multiple choice and short structured questions Performance and reporting of practical exercises Students work in groups to prepare a presentation and handout on current topics in Environmental Plant Physiology TEACHING STRATEGIES Credits: 3 made up as follows: Lectures: 17 h Tutorials: 7h Total = 24 contact hours (2 credits) Practicals: 24 practical hours (1 credit) (5 x 5 hours less 10 minutes = 24 hours) Lectures: Lectures will provide valuable synthesis and evaluation of the growing body of available information, update current issues, and prioritise content relevant to course assessment. Practicals: Fortnightly practicals will provide hands on experience for students to gain skills required for conducting well designed laboratory sampling and experiments; to problem solve and trouble shoot in real-life situations; and to become familiar the design and 252 implementation of ecophysiological experiments. Participation and performance in the practicals will be assessed as well as reports based on the practical activities. Tutorials: Tutorials will cover course topics in a highly interactive format using a variety of collaborative active learning techniques. Case study: The case study component is designed to be fully interactive with students researching and presenting their own case studies to the class. In each case the material provided in class will be complemented by notes and further reading provided through the myeLearning system. Students will also be encouraged to use the myeLearning forum in carrying out group work. myeLearning: myeLearning, will be used extensively during this course for: communication among students and staff (email, discussions) official posting of important notices (coursework assessment notices, instructions, and incourse results) provision of course details, lecture notes, practical guides, tutorial briefings provision of recommended resource materials and links to resources on specific websites Resources Many resources are available myeLearning including : Lecture presentations (including learning objectives) ‘General Resources’- direct links to videos, web-tools and publications relevant to the course Discussion forums Essential texts: Plant Ecology. 2005. Schulze, et al. Springer [QK901 .S38 2002] (In the Reserve Section, UWI, St Augustine, Main Library) Physiological Ecology of Tropical Plants, Luttge U. 2008. (Springer) [QK936 .L88 2008] (available as ebook from UWI Library) Texts from prerequisites: Plant Physiology. Taiz and Zeiger. 2010 (Sinauer Associates) Ecology: Instant Notes 2nd ed. Mckenzi etal. 2001 (Taylor & Francis) Texts for further reading: Environmental Physiology of Plants, A. Fitter and R. Hay. 2002. (Academic Press) [QK717 .F54 2002] Plants in Action, Atwell etal. 2010. http://sci-wikibook.bacs.uq.edu.au/edition1/ Physiological Plant Ecology. Larcher. 2003 (Springer) Plant Physiological Ecology. Lambers et al. 2008. (Springer)(available as ebook from UWI Library) The Physiology of Crop Yield. Hay and Porter .2006. (Blackwell Publishing) Practical equipment: Thermocouple, Quantum sensor, Psychrometer, Data loggers Infrared Gas Analyser, Chlorophyll florescence Psychrometer, Porometer, Pressure chamber 253 Conductivity meter, Micro balance, Drying ovens, Microwave ovens All equipment will be provide from the Dept. Life Sciences, except the Infrared Gas Analyser which will be demonstrated by the Dept. of Food Production. Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 254 COURSE CALENDAR Week Lecture 1 Practical Deadlines Introduction Stress physiology The light environment 2 The light environment Light stress Practical report 3 The thermal environment Temperature stress Heat stress 4 Water in the environment Water deficit 5 Tutorial: Introduction to the case study Practical report Tutorial: review Water stress 6 In-course Test I Ecophysiology in action (ecosystem services) 7 Habitat case study: mangroves Habitat case study: restoration ecology 8 Habitat case study: class presentation Habitat case study: class presentation 9 Habitat case study: class presentation Habitat case study: class presentation 10 Climate change (impacts) Climate change (ecosystem response) 11 Tutorial In-course Test II In-course Test I Practical report Climate & microclimate Casestudy handouts Practical report Diagnosing stress Practical report 12 In-course Test II Tutorial (streamed groups) Tutorial (streamed groups) 255 ADDITIONAL INFORMATION Refer to the Life Sciences Undergraduate Handbook for general guidelines for teaching, assessment, student conduct, essential skills, and support at http://sta.uwi.edu/fst/lifesciences/documents/handbook.pdf . Refer to the General Information and General Regulations in the Faculty Booklet available from http://sta.uwi.edu/resources/documents/facultybooklets/ScienceTechUndergrad.pdf . Students are reminded that they must attend a minimum of 75% of the practical sessions and tutorials. Failure to do so will result in debarment from the final examination. As a general principle, medicals or other excuses may only excuse a student’s presence at an assigned time. Students must still complete the assigned work (make-up lab report or make-up test) in order to obtain the marks for that item of coursework. The student is responsible for liaising with the Course Coordinator or Teaching Assistants to ensure the assigned make-up is completed. Students are hereby informed that plagiarism is forbidden and all unsupervised coursework items must be accompanied by a Coursework Accountability Statement in order to be assessed. Specific items may require submission through Turnitin on myeLearning. Refer to ‘University Regulations on Plagiarism’ available from http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf HOW TO STUDY FOR THIS COURSE: Attendance is mandatory for lectures, tutorials and practicals. Prior preparation is strongly advised to able to fully participate in activities and obtain the full value of the sessions. Thorough use should be made of the resources provided and students are strongly advised to become familiar with them and start utilising them from the first week. Regular updates on course progress and materials are also highly recommended and you should check into your UWI email and myeLearning on a frequent regular basis to review materials, assignments and activities. Students are encouraged to interact regularly with staff on their projects, even outside of the assigned tutorial times to ensure prompt, satisfactory solution of any problems and to monitor progress. ADF: 28 FEB 2013 THE UNIVERSITY OF THE WEST INDIES, ST. AUGUSTINE FACULTY OF SCIENCE & TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE OUTLINE COURSE CODE: BIOL3772 COURSE TITLE: Plant Development NO. OF CREDITS: 3 LEVEL: 3 SEMESTER: 2 256 PREREQUISITE(S): BIOL 2XXX Cell and Developmental Biology and either BIOL 2XXX Physiology of Plants or BIOL2761 Plant Physiology COURSE CAPACITY ENROLLMENT: 48 students (maximum) COURSE DESCRIPTION This course provides an advanced level focus on the molecular genetic, biochemical and physiological bases of plant development. Concepts of signal perception and transduction are initially reviewed. Students will be introduced to important experiments that have led to understanding many basic principles of plant development. Of particular importance is the use of mutation genetics as a tool to study development. Students in dissecting these experiments would be required to perform planned experiments and present their results and analysis in a group presentation format. COURSE RATIONALE BIOL3xxx is included in the Plant Biology option of the Biology degree. This course builds upon aspects of plant developmental biology and plant physiology taught in Year II of the programme. The student, upon completion of this course, should have a comprehensive understanding of the role of phytohormones in development, and the various developmental processes underlying the nature of some of the major plant organs. INSTRUCTOR INFORMATION Name of course coordinator: Dr. Georgette Briggs Office address and phone: Biochemistry Office, Floor 2, Old wing, Natural Sciences Building Email address: [email protected] Office hours: Wednesdays 2:00-4:00 pm Preferred method of contact: Email Communication policy: Students should use their UWI email account for communication and can expect a response within 48 hours. CONTENT The hormonal control of plant development is first examined by discussing in significant detail the biosynthesis, signalling and developmental effects on growth of each of the major plant hormones (ABA, GA, Brassinosteroids, Ethylene and Auxin). The second portion of the course, seeks to examine in detail the genetic control of development of all the major plant organs, including the seed, shoot, root and flower. GOALS/AIMS: On completion of this course, students should have: An understanding of the hormonal pathways and interactions that underlie fundamental processes operating during plant development An appreciation of the role of developmental processes in plant disease An understanding of the theory that underlies the genetic basis of plant developmental pathways 257 Developed laboratory skills in working with the model plant Arabidopsis thaliana The ability to interpret the outcomes of experimental scenarios Acquired the knowledge and ability to aid in critically evaluating scientific experiments and their findings Acquired specific analytical skills with respect to problems in plant development Gained skills in presenting and defending a logical, scientifically supported argument LEARNING OUTCOMES Students completing this course should be able to: - Relate the importance of the study of plant development to the fields of plant anatomy and plant physiology. - Explain the discovery, biosynthesis, forms, conjugates, bioassays, physicochemical measurement and effects of applied phytohormones on plants of commercial significance - Explain the processes and genetic interactions involved in the development of the seed coat, the seed, the flower, the root and the shoot of the plant. - Dissect the role of hormonal cross-talk and the effect it can have on devlopmental responses - Critically evaluate scientific research papers, assessing the relevance and importance of the scientific findings COURSE ASSESSMENT In-course test : Group research project - Group seminar presentation (10%) - Written report (15%) Written analysis of a scientific article Participation in online forums/Seminars Final examination (2 hours) 10% 25% 5% 10% 50 % Group research project (25%): Students work in groups of four and are assigned a research topic (Week 2), regarding one aspect of plant development. Students must design an experiment, perform that experiment and record data. The experiment must utilize one of the provided developmental mutants in Arabidopsis thaliana. A few key references are provided by the lecturer. These topics are discussed over the three sessions of student group meetings, where experimental design and analysis/interpretation of relevant research papers are the main focus. Written report (15%): The written report should reflect the experimental design, methodology, results and interpretation of results, of the experiments performed by the group. The complete report is due in Week 9. Seminar presentation (10%): The seminar presesntation would be 15-20 minutes duration, and would be presented to the class. These presentations would begin in Week 10 and should be completed in Week 12. Each group will also produce a one page summary of their topic for fellow students. These should be brought to the presentation session and will contribute to the presentation assessment. 258 Participation in online forums/Seminars (10%): Marks are awarded for student participation during seminar presentations. Students participation in schedulded online discussion forums are also awarded here. Written analysis of a scientific article (5%): This assignment is due in Week 12 and is a 1000 word analysis of an approved scientific research article that addresses one of the key plant development themes discussed in the lectures. EVALUATION The elected Class Representative and/or Deputy will attend meetings with the course teacher(s) organized at every 4th week, and present feedback from the students attending that course or stream. This feedback is normally provided both orally and in written form for transmission to the lecturer. Apart from that the representatives will be attending the Liaison Committee meeting, and give their feedbacks to the committee. Students may comment on any aspect of the course or facilities. Students will be encouraged to submit their feedback (oral/written) during tutorials directly to the course teacher and appropriate actions will be taken by the teacher then and there. Results of in-course tests, and other course assignments will be analyzed and presented in the class. This will help students to check their progress constantly and also helps the instructor to identify the weak areas and thereby could alert and advise students individually to alter their approach of study and completing the work. The final reflective feedback and comments about the entire course and teaching will be collected on the last day of the course. This will be saved for analysis and utilized as a base for improvement for the next offering in the following year. COURSE DELIVERY & TEACHING STRATEGIES The instruction for this course would comprise 2 lectures per week (50 mins each), and one tutorial session. In total, 24 one-hour lectures will be delivered, supplemented with 4 tutorial sessions, and 3 student group meetings. The student group meetings facilitate project discussion and journal discussions. The lecturer would be present at these sessions to guide the meetings. Theses are in-class oppurtunities for the lecturer to observe and guide the student group interaction and interpretation of the scientific research papers. Students would use these sessions to have confusing concepts clarified and to also submit up-to-date planning on experimental design for the project. Online forum discussions, seminar presentations and written reports would also be used to encourage student participation and collaborative learning. In addition students will have the opportunity to gain skills in oral communication, and collaborative group research and presentation. This format therefore addresses a range of learning styles, as outlined in the DLS undergraduate handbook. This course is also my e-learning supported, and many other resources are readily available for the students. RESOURCES Required reading: 259 Plant Physiology Taiz, L & Zeigler, E (2002) (3rd edition) Biochemistry and Molecular Biology of Plants Buchanan, B, Gruissem, W, and Jones, R . Mechanisms in Plant Development Leyser, O., & Day, S. (2009).. Chichester: John Wiley & Sons. Molecular genetics of plant development Howell, S. H. (2000).. Cambridge [u.a.: Cambridge Univ. Press. Developmental biology of flowering plants Raghavan, V. (2000).. New York, NY [u.a.: Springer. Recommended reading: Seed Coat Development and Dormancy, in Annual Plant Reviews Volume 27: Seed Development, Dormancy and Germination Debeaujon, I., Lepiniec, L., Pourcel, L. and Routaboul, J.-M. (2007) (eds K. J. Bradford and H. Nonogaki), Blackwell Publishing Ltd, Oxford, UK. doi: 10.1002/9780470988848.ch2 Lateral Root Formation, in Annual Plant Reviews Volume 37: Root Development Malamy, J. E. (2009) (ed T. Beeckman), Wiley-Blackwell, Oxford, UK..ch4 Arabidopsis Root Development Luijten, M. and Heidstra, R. (2009), in Annual Plant Reviews Volume 37: Root Development (ed T. Beeckman), Wiley-Blackwell, Oxford, UK.ch1 Further Reading: Key review papers and research papers would be used in discussion of the relevant topics. An example of the phytohormone Auxin reading list is included: 1. Bauly, JM et al. (2000) Overexpression of auxin-binding protein enhances the sensitivity of guard cells to auxin. Plant Physiol. 124: 1229-1238. 2. Chen, J-G, Shimomura, S, Sitbon, F, Sandberg, G & Jones, AM (2001) The role of auxinbinding protein 1 in the expansion of tobacco leaf cells. Plant J. 28: 607-617. 3. Chen, J-G, Ullah,H, Young, JC, Sussman, MR & Jones, AM (2001)ABP1 is required for organized cell elongation and division in Arabidopsis embryogenesis Genes Dev. 15: 902-911. 4. Dharmasiri, N & Estelle, M (2004) Auxin signaling and regulated protein degradation" TIBS 9: 302-308. 5. Ellis, B.E., Miles, G.P. (2001) "One for all?" Science 292: 2022-2023. 6. Galweiler, L, et al. (1998) Regulation of polar auxin transport by AtPIN1 in Arabidopsis vascular tissue. Science 282 2226-2230. 7. Hagen, G, Guilfoyle (2002) Auxin-responsive gene expression: genes, promoters and regulatory factors. Pl. Mol. Biol. 49: 373-385. 8. Hager, A., Debus, G., Edel, H.G., Stransky, H. & Serrano, R. (1991) Auxin induces exocytosis and the rapid synthesis of a high-turnover pool of plasma-membrane H+ ATPase. Planta 185 527-537. 9. Hertel, R (1995) Auxin binding protein 1 is a red herring. J Exp. Bot. 46: 461-462. 10. Jones, AL et al. (1998) Auxin-dependant cell expansion mediated by overexpressed auxinbinding protein 1. Science 282: 1114-1117. 11. Kepsinski, S, Leyser, O (2003) An axis of auxin. Nature 426: 132-135. 12. Kutschera, U. & Schopfer, P. (1985) Evidence against the acid-growth theory of auxin action Planta 163 483-493. 13. Leyser, O (2001) Auxin signalling: the beginning, the middle and the end. Curr. Opinion in Plant Biology 4: 382-386. 14. Napier, RM, David, KM, Perrot-Rechenmann, C (2002) A short history of auxin binding proteins. Pl. Mol. Biol. 49: 339-348. 260 15. Schindler, T., Bergfield, R., Hohl, M. & Schopfer, P. (1994) Inhibition of Golgi-apparatus function by brefeldin A in maize coleoptiles and its consequences on auxin-mediated growth, cell-wall extensibility and secretion of cell wall proteins. Planta 192 404-413. 16. Steinmann, T et al. (1999) Coordinated polar localization of auxin efflux carrier PIN1 by GNOM ARF GER. Science 286: 316-318. 17. Trewavas, A.J. & Cleland, R.E. (1983). AIs plant development regulated by changes in the concentration of growth substances or by changes in the sensitivity to growth substances?@ TIBS, 354-357. 18. Woo, E-J, Marshall, J, Bauly, J, Chen, JG, Venis, M, Napier, RM, Pickersgill, RW (2002) "Crystal structure of auxin-binding protein 1 in complex with auxin." EMBO Journal 21: 2877-2885. COURSE CALENDAR WEEK 1 2 3 4 5 6 LECTURE Lecture 1: Course introduction and Overview of plant development Lecture 2: Review of Signal Perception and Transduction Pathways. The classical hormone concept. Review of plant embryogenesis Lecture 3-4: Auxins – discovery, biosynthesis, natural vs synthetic auxins, conjugates, bioassays and physicochemical measurement, effects of applied auxins and commercial uses. Lecture 5-6: Gibberellins – discovery, biosynthesis, range of compounds, conjugates, bioassays and physicochemical measurement, effects of applied gibberellins. Commercial uses of gibberellins, gibberellin biosynthetic inhibitors and genetic engineering of gibberellin biosyntheis. Lecture 7-8: Cytokinins – discovery, biosynthesis, range of compounds, bioassays and physicochemical measurement. Effects of applied cytokinins and commercial uses. Lecture 9-10: Ethylene – discovery, biosynthesis, physicochemical measurement, effects of applied ethylene. Commercial uses of ethylene, ethylene biosynthetic inhibitors and genetic engineering of ethylene biosyntheis. Lecture 11-12: Asbscisic acid - discovery, biosynthesis, bioassay, physicochemical measurement, effects of applied abscisic acid. ACTIVITY Student group and topic assignments determined TUTORIAL STUDENT GROUP MEETING TUTORIAL Online discussion forum STUDENT GROUP MEETING TUTORIAL Online Exam 261 WEEK 7 8 9 10 11 12 LECTURE Lecture 13-14: Brassinosteroids- discovery, biosynthesis, bioassay, physicochemical measurement. Effects and uses of applied brassinosteroids. Lecture 15-16: Seed coat development, seed development & dormancy Lecture 17-18: The molecular analysis of Flower Development (the ABC model) Lecture 19-20: Roots – The initiation, growth and differentiation of roots Lecture 21-22: Shoots – stem, leaf & bud development Lecture 23-24: Cross-talk: Combinatoral and Conflicting effects of hormones on specific developmental processes. (e.g. Auxin and Ethylene; Auxin and Brassinosteriods) Abnormal plant growth (deregulation of hormonal processes). ACTIVITY STUDENT GROUP MEETING TUTORIAL Online discussion forum SUBMISSION OF WRITTEN REPORT GROUP PRESENTATIONS (X3) GROUP PRESENTATIONS (X3) GROUP PRESENTATIONS (X3) Written Analysis of Scientific Paper due. ADDITIONAL INFORMATION Student Attendance Attendance in the in-course exams and participation in the online discussions. Any student who misses any of the mandatory activities, is advised to consult immediately in person or by email with the course instructor regarding their make-up options. Absence must be accompanied by a written excuse or medical submitted to the Main office, Life Sciences within 7 days of the missed session. Any student who was inexcusably absent or who does not write an in-course exam or a quiz will receive 0% for that exercise. Students are strongly advised to atten the student group meetings and to participte in the seminars since these are marked activities. HOW TO STUDY FOR THIS COURSE Students are encouraged to work together in small cohesive groups as much as possible to go through the course content. As we go through the various topics, students should attend the tutorials which are on a weekly basis, and should ensure that they prepare by reading the relevant journals assigned per project group. All comments, questions and concerns provided on a particular topic will be addressed during the discussion segments tutorials, and during student group meetings. To support the material presented in class, several texts have been recommended. These texts, would also be supplemented by various scientific journal articles available to you via the myeLearning platform. Use the responses and comments from the online forum discussions, to clarify misconceptions on any topic, and to aid in your understanding of the topics. 262 Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 GB: 24 Jan 2013 THE UNIVERSITY OF THE WEST INDIES, ST. AUGUSTINE FACULTY OF SCIENCE & TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE OUTLINE COURSE CODE: COURSE TITLE: SEMESTER: LEVEL: NO OF CREDITS: BIOL 3773 Plant Anatomy 1 3 3 PRE-REQUISITES: Either BIOL1262 Living Organisms 1 or BIOL1261 Diversity of Organisms or (BIOL1065 Diversity of Plants and Animals and AGRI1012 Microbiology); and either BIOL 2XXX Physiology of Plants or BIOL2761 Plant Physiology Course Description 263 The course integrates developmental and functional aspects to explain the internal structure and external form of seed plants. The cells, tissues and organs, as well as their modifications, of representative plants are described. The roles of meristematic activity in primary and secondary growth and in determinate and indeterminate growth patterns are explained. Practical exercises are integrated with lectures as much as possible and emphasis is placed on hands-on specimen preparation and on effective use of the light microscope. The course will be assessed by means of in course theory and practical tests, practical reports, and a final theory exam. Purpose of the Course/Course Rationale Knowledge of plant anatomy is fundamental to the study of botany and of plant science in general, and the subject is no longer covered in other courses offered by the Department of Life Sciences Instructor’s Information Instructor: E-mail: Office location: Office hours: Dr. G. F. Barclay [email protected] Rm 324, Old Wing, Natural Sciences Building 9:00 - 5:00 daily (email for appointment) Course Content 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 1. 2. 3. 4. 5. Topics Meristems and growth patterns: primary and secondary growth Plant architecture and phyllotaxy. Transport tissues: phloem and xylem. Protective tissue layers: epidermis, exodermis, endodermis, and periderm. Support tissues: parenchyma, collenchyma, sclerenchyma, and xylem. Leaves. Functional design and modifications of structure. Root and stem structure and modifications. Organs of perennation. Wood anatomy. Grasses. Cane and corn plant structure. Flowers and their modifications. Pollination mechanisms. Fruit types, seed modification, and dispersal. Practical Exercises Cell types Anatomy of herbaceous stems and roots Leaves Woody stems and anomalous secondary growth Flowers and fruit Goals/Aims The aim of this course is to give students a working knowledge of the structure of seed plants. Learning Outcomes At the end of this course students ought to be able to: 1. Compare the anatomy of eudicot and monocot plants and the concepts of primary and secondary growth. 264 2. Distinguish between the different types of phyllotaxy and stem branching 3. Relate the anatomical and morphological features of leaves and their modifications to their functional characteristics. 4. Differentiate the external morphological root zones, root modifications, organs of perennation, pericycle, endodermis, casparian band. 5. Compare secondary growth in storage organs with typical secondary growth. 6. Correlate differences in floral structure with fruit structure, and pollination and seed dispersal strategies 7. Classify the organs, tissues, cell layers, and cell types observed in the practical sessions. 8. Create large well labeled illustrations of plant specimens and classify their tissues based on features observed. Course Assessment Component % Final Grade Final exam 50% In-course test 1 15% In-course test 2 15% Practical reports 10% Practical Quiz 10% Description 2-hour written exam with 20 multiple choice questions, and a choice of 2 out of 4 essay questions 10% theory and 5% based on Practicals 10% theory and 5% based on Practicals Performance and reporting of practical exercises Quizzes based on identification of plant tissues during practical Teaching Strategies Credits: 3 Lectures: 24 lectures Tutorials: 5 x 1 hour tutorials Practicals: (5x 3 hr practicals) Lectures: Lectures, which incorporate some video presentations, will provide valuable synthesis and evaluation of the growing body of available information, update current issues, and prioritize content relevant to course assessment. Practicals: Fortnightly practicals will provide hands on experience for students to gain skills required for conducting well designed laboratory sampling and experiments; to problem solve and trouble shoot in reallife situations; and to become familiar with Plant Anatomy. Tutorials: Tutorials will cover course topics in a highly interactive format using a variety of collaborative active learning techniques. myeLearning: myeLearning, will be used extensively during this course for: communication among students and staff (email, discussions) official posting of important notices (coursework assessment notices, instructions, and in-course results) 265 provision of course details, lecture notes, practical guides, tutorial briefings provision of recommended resource materials and links to resources on specific websites Resources Many resources are available including: Lecture presentations (including learning objectives) ‘General Resources’- direct links to videos, web-tools and publications relevant to the course Discussion forums Essential texts: Esau, K. 1977. Anatomy of Seed Plants. 2nd ed. New Jersey and London: John Wiley & Sons, Inc. Class Text: G. F. Barclay. Plants Alive! Online course resource: http://sta.uwi.edu/fst/lifesciences/bl11f/ Texts for further reading: Evert, R.F. 2006. Esau's Plant Anatomy. Meristems, Cells, and Tissues of the Plant Body: their Structure, Function, and Development. 3rd ed. New Jersey and London: John Wiley & Sons, Inc. Dickison WC. 2000. Integrative Plant Anatomy. New York: Academic Press. Bell, A.D., 2008. Plant Form: An Illustrated Guide to Flowering Plant Morphology. Portland and London: Timber Press Beck C B (2010) An Introduction to Plant Structure and Development: Plant Anatomy for the Twenty-First Century. 2nd ed. Cambridge: Cambridge University Press Mauseth J D (1988) Plant Anatomy. Menlo Park, CA: Benjamin/Cummings Peterson, R. L. Peterson, C. A. Melville L. H. 2008. Teaching Plant Anatomy Through Creative Laboratory Exercises. Ottawa, Ontario: NRC Press COURSE CALENDAR Week 1 Lecture Course Introduction Structure of a typical plant Lab Tutorial 2 Vascular tissues Support tissues Cell types Tutorial 1 3 Protective tissues Root and stem structure 4 Plant Architecture Phyllotaxy 5 Leaves 1 Leaves 2 6 Review Deadlines Practical report Stems and roots Tutorial 2 Practical quiz Practical report Leaves Tutorial 3 266 Theory and practical test 1 In-course test 1 7 Modifications of roots, stems, and leaves 1 Modifications of roots, stems, and leaves 2 Practical report 8 Secondary growth Trees and wood anatomy 9 Grasses Crop plant anatomy and morphology 10 Flowers and pollination Fruit structure and seed dispersal 11 Review Theory and practical test 2 12 Review Review Secondary growth Tutorial 4 Practical quiz Practical report Flowers and fruit Tutorial 5 Practical report In-course test 2 ADDITIONAL INFORMATION: Refer to the Life Sciences Undergraduate Handbook for general guidelines for teaching, assessment, student conduct, essential skills, and support at http://sta.uwi.edu/fst/lifesciences/documents/handbook.pdf . Refer to the General Information and General Regulations in the Faculty Booklet available from http://sta.uwi.edu/resources/documents/facultybooklets/ScienceTechUndergrad.pdf . Students are reminded that they must attend a minimum of 75% of the practical sessions and tutorials. Failure to do so will result in debarment from the final examination. As a general principle, medicals or other excuses may only excuse a student’s presence at an assigned time. Students must still complete the assigned work (make-up lab report or make-up test) in order to obtain the marks for that item of coursework. The student is responsible for liaising with the Course Coordinator or Teaching Assistants to ensure the assigned make-up is completed. Students are hereby informed that plagiarism is forbidden and all unsupervised coursework items must be accompanied by a Coursework Accountability Statement in order to be assessed. Specific items may require submission through Turnitin on myeLearning. Refer to ‘University Regulations on Plagiarism’ available from http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf HOW TO STUDY FOR THIS COURSE: Attendance is mandatory for lectures, tutorials and practicals. Prior preparation is strongly advised to able to fully participate in activities and obtain the full value of the sessions. Thorough use should be made of the resources provided and students are strongly advised to become familiar with them and start using them from the first week. Regular updates on course progress and materials are also highly recommended and you should be checking into myelearning on a frequent regular basis to review materials, assignments and activities. Students are encouraged to interact regularly with staff on their projects, even outside of the assigned tutorial times to ensure prompt, satisfactory solution of any problems and to monitor progress. Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 267 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 GFB: 20 Jan 2013 rev 28 Feb 2013 THE UNIVERSITY OF THE WEST INDIES, ST. AUGUSTINE FACULTY OF SCIENCE & TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE DOCUMENTATION COURSE CODE: BIOL 3866 COURSE TITLE: Parasite Biology NO. OF CREDITS: 3 LEVEL: III PREREQUISITES: BIOL1263 Living Organisms II or BIOL1261 Diversity of Organisms or (BIOL1065 Diversity of Plants and Animals and AGRI1012 Microbiology) and either BIOL 2XXX Physiology of Animals or BIOL2862 Animal Physiology ANTI-REQUISITE: BIOL2864 - Parasitism 268 COURSE DESCRIPTION The course Parasite Biology is divided as follows: The study of individual parasites: It is only through the study of a parasite’s biology and functions that steps can be taken to fight it. The study of host-parasite relationships: Disciplines which investigate how the host and parasite(s) interact include Physiology, Biochemistry, Cell Biology, and Pharmacology. Immunology: This deals with the immunological response that is triggered in the host and the ways in which the parasite attempts to evade it. Disciplines include Cellular and Molecular Immunology. Chemotherapy: This area investigates the effect of drugs on both the parasite and the host, as well effective treatments to ensure the death of the parasite and the recuperation of the host. Disciplines include Organic Chemistry, Pharmacology, Biochemistry and Medicine. Epidemiology: This field looks at the spread of parasitic diseases through study of the host, parasite and vectors. Disciplines include Tropical Hygiene, Entomology and Geographical distribution. This course will be taught using a mixture of lectures, seminars and projects, team oral presentations, individual essays, reading materials and seminar-style classes, laboratory session to reinforce lectures and for hands on experience identifying, understanding form and function, and evolutionary processes. Course assessment will be based on a student seminar and an essay on current topics in parasitism together with lab exercises on form and function, and evolutionary processes. A final examination will be used to ensure student learning objectives are achieved. COURSE RATIONALE Parasitism is a very successful way of life. All of the major groups of animals have parasitic members and 50% of all known animal species are parasitic at some stage of their life cycle. One of the fascinations of parasites is the complexity of their life cycles, involving one, two, three or even four consecutive hosts and alternating between vertebrate hosts and invertebrate hosts and between terrestrial and aquatic environments. Therefore parasitic adaptations are responses to features in the parasite environment and this environment is the body of another organism, the host.In addition, approximately 15 million people still die each year from infectious diseases; most living in developing countries. Six diseases are considered most detrimental to human health by the World Health Organization, of which five are caused by parasites and are vector-borne. Consequently, control of parasites should be integrated with the life cycle of the parasite, the host and the environment. INSTRUCTOR INFORMATION Prof. Dave D. Chadee Natural Sciences Bldg New Wing Room 216, phone 662-2002 ext 83740 [email protected] Office hours: Monday and Tuesday from 9.00-1200 Noon LETTER TO THE STUDENT Dear Student, 269 Welcome to BIOL 3…. The journey from the first day of class to your final day will be filled with awe and wonder of how such small organisms have adapted and survived for millennia. In addition, we will study of the relationship between two specific types of organisms, i.e. the parasite and the host. We will explore the evolutionary aspects of all these interactions between organisms, that is, when there is an exchange of food and shelter. I will therefore guide you through this process and the understanding of parasitism as a way of life. Sincerely, Dave D. Chadee Professor of Environmental Biology CONTENT: (1) Fundamental concepts of Parasitology: morphology, lifecycle, transmission, pathology and control of selected protest, helminth and arthropod parasites of humans and domesticated animals: laboratory diagnostic techniques, parasite ecology and evolution, parasite immunology; epidemiology of ectoparasite infections in the Caribbean region. These concepts will be taught through lectures on the following topics: Introduction to Protozoan parasites including Malaria, African and American Trypanosomiasis and Leishmaniasis; Parasites of the Intestines including Nematodes, Cestodes and Trematodes; Life Cycles of Parasites; Host Specificity, Parasite Immunity, Parasite Diagnosis and Control; Adaptation/ Evolution of Parasitism, Ectoparasites; Environmental Parasitology; Evidence Based Parasitology. Practical classes take place in both laboratory and field. Reading in Parasitism and the development of evolutionary adaptation hypotheses, a laboratory manual will be used to facilitate the learning process. (2) Laboratory-based exercises will include identification/diagnosis of a range of parasite infections of humans and domesticated animals GOALS/AIMS The course aims to provide a solid grounding in both theory and practice of parasite biology. The goals of the Lectures and laboratory sessions are to: 1. study individual parasites, their life cycles, characteristic features as they relate to function 2. study host-parasite relationships and the role physiology, biochemistry, cell biology and pharmacology plays in establishing these relationships 3. study the immune response that is triggered in the host and the way in which these parasites attempt to evade it 4. investigate the effects of chemotherapy on both parasites and host 5. determine the susceptible stages of these parasites for developing control/intervention strategies 6. examine the spread of parasitic diseases through the study of host-parasite-vector relationships 7. examine the evolutionary processes which have lead to the success of parasites and parasitism as a way of life 8. review parasites found in the Caribbean and Latin American region. 270 LEARNING OUTCOMES At the end of this course, students will be able to: 33. Characterize the global diversity of parasites and discuss putative reasons for their diversity. 34. Define and describe parasitism, mutualism, symbiosis and endosymbiosis to convey a basic understanding of these topics. 35. Describe, analyze and discuss the main features of parasite-host relationships. 36. Analyze and access the reasons for parasites success and survival. 37. Outline, analyze and evaluate the different parasites life cycle strategies. 38. Characterize the major ectoparasites found in the Caribbean region and compare them with respect to gross structure and way of life. 39. Characterize, analyze and access the immunological response that is triggered in response to parasites, the use of diagnostic methods and development of control strategies. ASSIGNMENTS The theory coursework, accounting for 10% of total marks, will be a Seminar and an Essay on current topics of parasitism and will be allotted in mid February. Essay submission by each student will be at the end of the semester. COURSE ASSESSMENT Practical coursework Lab book ... 25% Theory coursework One in-course test ... 5% Assigned Seminar/essay account ... 10% Final theory exam ... 60% EVALUATION The Department of Life Sciences takes the quality control of its courses very seriously. Students who have comments on one or another aspect of a course are encouraged to communicate these to the instructors. More formal feedback is sought through the Staff-Student Liaison Committee, which meets twice per semester. This departmental committee comprises elected student representatives from each course in the given semester. In addition, the university conducts a confidential written evaluation of most courses and their instructors toward the end of each semester. TEACHING STRATEGIES Teaching methods Lectures, Projects using the Team Approach, Team Oral presentations, Individual Essays, Reading materials and Seminar Style Class, Laboratory session to reinforce lectures and for hands on experience identifying, understanding form and function, and evolutionary processes. A combination of teaching strategies will be use including 271 Case studies Interactive Lectures Seminars Projects Group Discussions Learning Strategies Interviews Internet Library literature search myeLearning, The course comprises 18 one-hour lectures, six one-hour tutorials and eight three-hour practical sessions. Tutorials are about collective problem solving with minimal lectures-review by the instructor. However, lectures always have a certain tutorial aspect, so that the instructor will often pose questions to members of the class and will expect answers. Where a given topic arises both in a practical exercise and in lecture, every effort is made to provide answers. Students are expected to read widely especially the materials available in the university library, on the internet and on myeLearning, as an extension of the lecture notes. RESOURCES Suitable reading matter can be found in the university library, on the internet and on myeLearning. The library has several relevant textbooks of general Parasitology and a good set of books on the taxonomy, ecology and behaviour of parasites. Practical exercises in the lab will utilize a wide range of standard equipment and materials. The course manual and laboratory manual are placed on MyElearning READINGS 1. Prescribed: Roberts LS and Janovy J (2009). Gerald D. Schmidt and Larry S. Roberts’ Foundations of Parasitology. 8th Edition. McGraw-Hill Science/Engineering/Math. ISBN-10: 0073028274 2. Highly recommended :Marquardt WC, Demaree RS and Grieve RB (2000). Parasitology and vector biology 2nd Edition. Academic Press. ISBN 0124732755, 9780124732759 3. 3. Online resources http://www.med.sc.edu:85/book/parasit-sta.htm 272 COURSE CALENDAR Week Practical 1 2 3 4 Lectures/tutorial Assignments Introduction to the course, Course Lab report due overview and course requirements The nature of parasites and their success Tutorial: Estimating the global species richness of parasites Morphology of the Introduction to Protozoan parasites Lab report due different groups of including Malaria, African and parasites American Trypanosomiasis and Leishmaniasis Parasites of the Intestines including Nematodes, Cestodes and Trematodes Tutorial: The impact of these parasites on health and wellbeing Geographical Life Cycles of Parasites Lab report due distribution parasites and host 5 of their Host Specificity Tutorial: Parasite Adaptation to Hosts the Parasite Immunity Lab report due 6 Investigate different stages of the life cycle of parasites 7 Parasite Diagnosis and Control; Tutorial: What is evidence based Parasitology Determine the various Ectoparasites - Ticks and Mites 8 In-course test Lab report due mechanisms of infections and special features 9 10 Ectoparasites-Fleas and Tungiasis Tutorial: Ectoparasitic insects and Environmental Parasitology the Compare contrast different morphological features and their adaptation to different environments Lab report due 273 11 12 13 Evolutionary survey of major parasite orders Tutorial: Developing control strategies Investigate the Seminar/Lecture - Selected Topic Assigned various diagnostic account methods available for identification of parasites Review of the lectures, clarifying course issues and reviewing holistically the course content. Seminar/essay ADDITIONAL INFORMATION Attendance is not recorded at lectures and tutorials, but missing them is not a good idea. Attendance at practical exercises is mandatory, and the student should report any excusable absence promptly (in advance, if this is feasible). HOW TO STUDY FOR THIS COURSE Lectures provide a necessary focus for studying, but they do not by any means provide the entirety of what should be learned. Rather, the student should take lecture material as a point of departure in extensive outside reading. This supplemental material deepens one’s understanding of principles treated rather summarily in lecture and provides illustrations of these principles. It is a good idea to bring your copy of the course manual not only to lab but to lecture as a matter of course. It includes summaries of all lectures and a number of illustrations and appendices to which reference will be made in lecture. ADDITIONAL INFORMATION Refer to the Life Sciences Undergraduate Handbook for general guidelines for teaching, assessment, student conduct, essential skills, and support at http://sta.uwi.edu/fst/lifesciences/documents/handbook.pdf . Refer to the General Information and General Regulations in the Faculty Booklet: http://sta.uwi.edu/resources/documents/facultybooklets/ScienceTechUndergrad.pdf. Attendance is compulsory and an attendance register will be kept for these sessions. Failure to attend may result in a 10% deduction of marks from the assessment assignments. Monitoring time spent on the project. Students are advised to arrange exact dates with their supervisors and to keep a record of key discussion points and decisions. Time spent on the project activities should be recorded. Students are hereby informed that plagiarism is forbidden and all unsupervised coursework items must be accompanied by a Coursework Accountability Statement in order to be assessed. Specific items may require submission through Turnitin on myeLearning. Refer to ‘University Regulations on Plagiarism’ available from http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf 274 Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 THE UNIVERSITY OF THE WEST INDIES, ST. AUGUSTINE FACULTY OF SCIENCE AND TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE OUTLINE COURSE CODE: BIOL3867 COURSE TITLE: Animal Behaviour NO. OF CREDITS: 3 LEVEL: Undergraduate – Level 3 PRE-REQUISITES: BIOL1263 Living Organisms II or BIOL1261 Diversity of Organisms or (BIOL1065 Diversity of Plants and Animals and AGRI1012 Microbiology) and either BIOL 2XXX Physiology of Animals or BIOL2862 Animal Physiology 275 ANTI-REQUISITE: BIOL3861 Animal Behaviour ENROLMENT: Maximum 70 students COURSE DESCRIPTION: This terminal course examines the several complementary approaches (ontogeny, phylogeny, function, and causation) that have been used in the study of animal behaviour and the major ideas that have been developed, many of which have spread to other areas of biology. This course builds on previous offerings of the Department in ecology and zoology, to complete the study of animal biology for students taking the Zoology option at level III. An introduction is given to social behaviour, which is expanded on in other level III courses in the zoology option, on humans and insects. A solid background in the structure and function of the nervous, sensory, and endocrine systems in vertebrates and invertebrates is required to understand the mechanisms of animal behaviour. In addition, students should have a basic understanding of animal ecology, for example from the introductory course BIOL1462 General Ecology and Biometry or BIOL2xxx Fundamentals of Ecology, particularly in evolution, energetics, and population ecology. The course is organised into lectures and tutorials covering general and specific concepts in animal behaviour. In tutorials students are expected to prepare, participate and perform in an active way in order to engage with the content. Assessment will be based on a research essay, practical reports, and a final theory exam. COURSE RATIONALE: This is a core course for the Zoology option in the Department of Life Sciences and is an essential area in the study of animal biology. Animals are the most complex objects in the known universe, and behaviour is their major emergent feature. At the end of the course, students are expected to have an understanding of the principles sufficient to pursue research in animal behaviour or associated subjects such as biodiversity, conservation, or animal welfare. INSTRUCTOR INFORMATION: Name of instructor: Dr A. Hailey Office address and phone: Room 226, Zoology Section, New Wing, Natural Sciences Building. Phone ext 82206 [email protected] Email address: Office hours: Monday, Wednesday, Thursday, 11-12 a.m., other times by appointment Preferred methods of contact: Email or myelearning Academic Forum Communication policy: Matters concerning the individual student should be raised by email, from the student’s UWI email account. Matters of interest to the class should be raised as Academic Forum postings on myelearning. In either case students can expect a response within 48 hours. DO NOT use myelearning messaging, which is only supported via email and after some delay. CONTENT: General aspects of animal behaviour Approaches to animal behaviour History of the study of animal behaviour 276 Methods of studying animal behaviour Evolution of behaviour Physiological behaviour Perception Processing Organization of behaviour Ecological behaviour Feeding Defence Territory and social behaviour Learning and ontogeny Instinct and learning Types of learning Learning and the development of behaviour Communication Communication and signals Evolution of signals Complex communication - language Reproductive behaviour Reproductive strategies Mating systems Parental care GOALS/AIMS: This course aims to Introduce students to the major subject and growth areas of animal behaviour Relate animal behaviour to other aspects of animal biology, especially ecology, physiology, and diversity Give students a sound grasp of the scientific method, sufficient to begin to develop and test hypotheses in animal behaviour LEARNING OUTCOMES: Lectures are grouped into six major subject areas of animal behaviour, at the end of which the student should be able to 1. Plan and carry out a study of animal behaviour, as stand-alone work or as part of a broader biological enquiry (General) 2. Describe how physiological mechanisms produce behaviour in the individual (Physiological behaviour) 3. Use the principles of animal behaviour to investigate the function of animals in their environment, including an introduction to social behaviour (Ecological behaviour) 277 4. Evaluate the complementary approaches of instinct and different types of learning to the acquisition of behaviour by the individual (Learning and ontogeny) 5. Describe the principles of communication between individuals of the same and different species (Communication) 6. Evaluate behavioural strategies as components of reproduction and associated activities of animals (Reproduction) ASSIGNMENTS: Practicals 11. Readings in animal behaviour, and formulation of hypotheses (video/library) 12. Chemoreception, vision and orientation (laboratory) 13. Foraging behaviour in ants (outdoor, UWI) 14. Describing behaviour: Captive land vertebrates (zoo) 15. Social facilitation in chicks (laboratory) Research Essay A species behaviour account for the Online Guide to the Animals of Trinidad and Tobago (OGATT) (http://sta.uwi.edu/fst/lifesciences/ogatt.asp). Submitted through myelearning, with a limit of 3% similarity as determined by Turnitin (excluding references). Students may submit drafts to Turnitin from week 3 up to the deadline (end of week 11) and view their Turnitin reports to correct any similarity in excess of this value. The final version remaining at the submission deadline will be marked. COURSE ASSESSMENT: Coursework practical: Coursework theory: Final examination: 25%, based on the best 4 of the 5 practical marks from the written reports and marks for participation. Due 7 days after the class 25%, from the research essay. Instructions given in weeks 2 and 3, final essay due at the end of week 11 through Turnitin on myelearning. 50%. The examination is of 2 hours duration. It is based on essay questions, with any two (2) questions to be completed from a choice of five (5). EVALUATION: Feedback on the course will be obtained informally from students on an ongoing basis by regular interactions and meetings among students, demonstrators, teaching assistants and the Course Coordinator in practicals and tutorials. Formal feedback will be via election of Class Representatives who sit on the Departmental StudentStaff Liaison Committee meetings held twice during the semester. Class reps will channel both concerns and commendations to the meeting as guided by the Department’s Standard Operating Procedures. Formal evaluation of the entire course will be accomplished via a UWI Course Evaluation questionnaire administered anonymously and confidentially at the end of the semester. All feedback will be considered on an ongoing basis and corrective action or adjustments made or discussed with students promptly or incorporated the following year. TEACHING STRATEGIES: Credits: 3 Lectures: 18 hours Tutorials: 6 hours Practicals: 24 hours 278 Lectures and tutorials are given to the whole class, and practicals are usually delivered to the students in two (2) streams. The course outline, objectives, readings, practical schedules, and instructions for the research essay are available on myelearning, together with the opportunity for feedback through the Academic Forum. Lecture materials are reduced to promote understanding of principles rather than transmission of facts, in particular with diagrams simplified to the essentials. Practicals build in sequence from initial observation and hypothesis formulation, laboratory experiments in physiological behaviour, through observational studies in a zoo setting and in the field, to the design of group experiments to test original hypotheses on campus. Practical schedules are available before the class to maximise usefulness of the class time. RESOURCES: Texts: Alcock, J. (2005). Animal Behavior: An Evolutionary Approach. 8th edition (6th-9th editions are useful). Sunderland, MA: Sinauer. Manning, A. & Dawkins, M.S. (2012). An Introduction to Animal Behaviour. 6th edition. Cambridge: Cambridge University Press. [Available in the campus bookshop] Ryan, M .J. & Wilczynski, W. (2011). An Introduction to Animal Behaviour: an Integrative Approach. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press. [Available in the campus bookshop] Search engine: http://scholar.google.com/ is given as the preferred source of references for the research essay, as it accesses academic papers rather than web sites. READINGS: Notice to the student: These readings have been selected as background material for the course. Each corresponds to a numbered lecture, and should be read before the relevant lecture for greatest usefulness. Where possible, primary sources (i.e. research papers) have been given, to show the type of material that you should be using as references in your own work (lab reports, and particularly the research essay). You should also use the same format for referencing (without giving notes). The notes also show which readings are used by one of the recommended texts, so you can compare the form in which information reaches the textbooks with the original sources: Alcock (A) or Manning & Dawkins (MD). 1. Tinbergen, N. (1963). On aims and methods of ethology. Z. Tierpsychol. 20: 410-433. The classic paper on approaches to animal behaviour. A, MD 2. Lehner, P. N. (1987). Design and execution of animal behavior research: an overview. J. Anim. Sci. 65: 1213-1219. A short summary review, focusing on domesticated animals, of a subject on which the author (Lehner, 1979, in literature cited) and others (Martin and Bateson, 1986) have written books. 3. Maynard Smith, J. & Price, G. R. (1973). The logic of animal conflict. Nature 246: 15-18. A short but difficult theoretical paper, the first use of game theory in animal behavior. MD 4. McComb, K., Reby, D., Baker, L., Moss, C. & Sayialel, S. (2003). Long-distance communication of acoustic cues to social identity in African elephants. Anim. Behav. 65: 317-329. A recent paper on perception that relies on good experimental design rather than on advanced techniques or statistics. 279 5. Ewert, J.-P. (1980). Neurobiological basis for the recognition and localization of environmental signals: how does a toad brain recognize prey and enemy? Chapter 4 in Neuroethology. Berlin: Springer-Verlag. The primary literature on neural processing in behaviour is specialized, so a secondary source is used: part of a chapter in Ewert’s monograph, describing his own work on toads. MD 6. Page, T. L., Caldarola, P. C. & Pittendrigh, C. S. (1977). Mutual entrainment of bilaterally distributed circadian pacemakers. Proc. Natl. Acad. Sci. USA 74: 1277-1281. A fairly accessible paper on the effects of optic lobe lesions on the circadian rhythm of a cockroach. 7. Cristol, D. A. & Switzer, P. V. (1999). Avian prey-dropping behavior. II. American crows and walnuts. Behav. Ecol. 10: 220-226. Testing the predictions of an optimal foraging model. A 8. Burger, J. & Gochfeld, M. (2001). Smooth-billed ani (Crotophaga ani) predation on butterflies in Mato Grosso, Brazil: risk decreases with increased group size. Behav. Ecol. Sociobiol. 49: 482492. Group-living may also function as a defence against predation. A 9. Wilkinson, G. S. (1984). Reciprocal food sharing in the vampire bat. Nature 308: 181-184. The classic study of reciprocal altruism. A, MD 10. Jacobs, L. F., Gaulin, S. J., Sherry, D. F. & Hoffman, G. E. (1990). Evolution of spatial cognition: sex-specific patterns of spatial behavior predict hippocampal size. Proc. Natl. Acad. Sci. USA 87: 6349-6352. Sexual differences in (spatial) learning ability in small mammals are associated with differences in brain anatomy; the size of the hippocampus. A 11. Povinelli, D. J. & Dunphy-Lelii, S. (2001). Do chimpanzees seek explanations? Preliminary comparative investigations. Can. J. Exp. Psychol. 55: 187-195. A paper from comparative psychology directly comparing chimpanzees and children. Chimpanzees show insight learning, but do not go the further step of theorizing, which is restricted to man. 12. Marler, P. & Tamura, M. (1964). Culturally transmitted patterns of vocal behavior in sparrows. Science 146: 1483-1486. Song development in birds as a specialized form of learning. Note the small sample sizes and lack of statistics in this old but classic paper. A, MD 13. Andersson, M. (1982). Female choice selects for extreme tail length in a widowbird. Nature 299: 818-820. Experiments on the function of long tails as signals in male birds. A, MD 14. Briskie, J. V., Martin, P. R. & Martin, T. E. (1999). Nest predation and the evolution of nestling begging calls. Proc. R. Soc. Lond. B 266: 2153-2159. Nestling begging calls are related to the risk of predation among 24 species of forest birds. A 15. Seyfarth, R. M., Cheney, D. L. & Marler, P. (1980). Monkey responses to three different alarm calls: evidence of predator classification and semantic communication. Science 210: 801-803. Vervet monkey alarm calls as a form of language. MD 16. Clutton-Brock, T. H. & Vincent, A. C. J. (1991). Sexual selection and the potential reproductive rates of males and females. Nature 351: 58-60. Competition among females for males in species with sex role reversal. A 17. Westcott, D. & Smith, J. N. M. (1997). Lek size variation and its consequences in the ochrebellied flycatcher, Mionectes oleaginous. Behav. Ecol. 8: 396-403. Both hotshot and hot-spot models of lek formation may operate simultaneously. A 18. Emlen, S. T., Demong, N. J. & Emlen, D. J. (1989). Experimental induction of infanticide in female wattled jacanas. Auk 106: 1-7. 280 Female infanticide in a bird with sex role reversal. MD COURSE CALENDAR: Week 1 Lectures and Tutorials 2 3 4 5 6 7 8 9 10 11 12 13 Introduction to course (Tutorial) Approaches to animal behaviour Methods of studying animal behaviour History of the study of animal behaviour (Tutorial) Evolution of behaviour Discussion of research essay (Tutorial) Perception Processing Organization of behaviour Tutorial Feeding Defence Territory and social behaviour Instinct and learning Types of learning Learning and the development of behaviour Tutorial Communication and signals Evolution of signals Complex communication language Reproductive strategies Mating systems Parental care Tutorial Course review Practicals Assignments due Readings in animal behaviour Practical 1 Chemoreception Practical 2 Foraging behaviour Practical 3 Captive land vertebrates Practical 4 Social facilitation Research Essay Practical 5 281 ADDITIONAL INFORMATION: Refer to the Life Sciences Undergraduate Handbook for general guidelines for teaching, assessment, student conduct, essential skills, and support at http://sta.uwi.edu/fst/lifesciences/documents/handbook.pdf . Refer to the General Information and General Regulations in the Faculty Booklet: http://sta.uwi.edu/resources/documents/facultybooklets/ScienceTechUndergrad.pdf. Students are reminded that they must attend a minimum of 75% of the practical sessions and tutorials. Failure to do so will result in debarment from the final examination. As a general principle, medicals or other excuses may only excuse a student’s presence at an assigned time. Students must still complete the assigned work (make-up lab report or make-up test) in order to obtain the marks for that item of coursework. The student is responsible for liaising with the Course Coordinator or Teaching Assistants to ensure the assigned make-up is completed. Students are hereby informed that plagiarism is forbidden and all unsupervised coursework items must be accompanied by a Coursework Accountability Statement in order to be assessed. Specific items may require submission through Turnitin on myeLearning. Refer to ‘University Regulations on Plagiarism’ available from http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf HOW TO STUDY FOR THIS COURSE: Attendance is mandatory for lectures, tutorials and practicals. Prior preparation is strongly advised to able to fully participate in activities and obtain the full value of the sessions. Thorough use should be made of the resources provided and students are strongly advised to become familiar with them and start utilising them from the first week. Regular updates on course progress and materials are also highly recommended and you should be checking into myelearning on a frequent regular basis to review materials, assignments and activities. Students are advised to read more than one of the many texts in animal behaviour available in the Alma Jordan Library and the campus bookshop. The approaches of authors to the subject vary widely, from the mechanistic (Ryan and Wilczynski is the latest of several) to the ecological (e.g. Alcock) and the zoological/ethological (e.g. Manning and Dawkins). Students will learn best by comparing these approaches and learning the important ideas in animal behaviour by reinforcement, while seeing a range of examples and viewpoints, rather than cramming from a single source. Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 282 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 AH 28 February 2013 THE UNIVERSITY OF THE WEST INDIES, ST. AUGUSTINE FACULTY OF SCIENCE & TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE DOCUMENTATION Course code: BIOL3868 Course Title: THE ECOLOGY OF HUMANS Credits: 3 Level: 3 Semester: 1 Pre-requisites: Either BIOL 1263 Living Organisms II or BIOL1261 Diversity of Organisms or (BIOL1065 Diversity of Plants and Animals and AGRI1012 Microbiology) and either BIOL 2XXX Fundamentals of Ecology or BIOL1462 General Ecology and Biometry. Anti-requisite: BIOL2461 Humans & the Environment COURSE DESCRIPTION: This course focuses on one of the most important animals on Earth today, Homo sapiens, considering the species from a broad biological and ecological perspective. The course introduces the evolution and origin of modern humans, the extent of their uniqueness in comparison with other animals and Primates, and the characteristics that contribute to their unprecedented success and dominance of their environment. We also explore selected 283 aspects of human biology and ecology including genetic and cultural diversity and adaptation; technological and lifestyle changes and their relationship with health and disease patterns; human populations, resources and wellbeing; resource depletion, environmental degradation and global climate change. In conclusion we discuss the future of the human animal. Students are expected to have a basic foundation in ecology and biodiversity. In addition to providing a foundation of theoretical knowledge, this course take a ‘hands-on’ approach where students are expected to prepare, participate and perform in an active way in order to engage with the content in a variety of ways. Assessment is designed to encourage students to work continuously with the course materials, explore and critically analyse research in this complex and rapidly developing field. Students are expected to have a basic foundation in animal biology. PURPOSE OF THE COURSE/RATIONALE: This is a core course for the Zoology specialisation in the Department of Life Sciences and is essential background for environmental sciences, environmental biology and environmental management. It also broadens the scope of studies in biology, plant biology and zoology. The course is an elective in the Department of Life Sciences and augments the Biology major and Ecology & Environmental Biology specialisation. Human actions threaten the very ability of the planet to support themselves and other organisms. It allows students to look critically at the past and present circumstances of the species to which they belong and craft solutions to face the future. INSTRUCTOR INFORMATION: Name of course coordinator: Mary Alkins-Koo Office address and phone: Room 218, Zoology offices, Floor 2, New wing, Natural Sciences Building Email address: [email protected] Office hours: Mondays 10-11am, Fridays 11-12am Preferred method of contact: Email Communication policy: Students should use their UWI email account for communication and can expect a response within 48 hours. LETTER TO THE STUDENT: Welcome to ‘The Ecology of Humans’. In this course you will be introduced to the species to which you belong. You will investigate it using a broad biological and ecological perspective to better understand our role on Earth as a dominant species, our relationships with our environment and other species, and scenarios for the future. The course will be taught in blended mode comprising both face-to-face and online delivery. You are expected to engage with the materials provided online, in face-to-face lectures and tutorials as well as explore and interact with these to develop the essential skills of critical thinking (clarity, accuracy, relevance, logic, breadth, depth, precision, significance, completeness and fairness). Activities will be diverse and you are strongly encouraged to ask questions, offer new ideas, problem solve and think innovatively to maximise the value of your learning experience. Please read the Course Outline carefully and keep the Course Schedule close to hand as it contains all the activities and assessments during the semester. In particular take note of the policy on plagiarism and attendance requirements. We are here to ensure your success in this course and at UWI generally and encourage you to come to us with any academic or other challenges you may face that could affect your attendance and performance. 284 I look forward to meeting each of you and engaging in productive stimulating discussions over the coming semester. Dr Mary Alkins-Koo. Course Coordinator CONTENT: Topics to be covered in this course include: What makes us human? The human Primate The evolution and origin of modern humans Anatomy and bipedalism Culture, technology and lifestyles Genetic and cultural diversity and adaptation The brain, language and intelligence Sex, reproduction and life history Social behaviour and organisation Human populations Human well-being and inequity Human health and disease Resources for the future Environmental degradation Global climate change The future of the human animal GOALS/AIMS: This course aims to Study the human animal from a broad biological and ecological perspective Evaluate the features that contribute to our unprecedented success and dominance as a species Explore the major issues facing the species for future survival and consider potential broad approaches to deal with some of these issues GENERAL OBJECTIVES The general objectives of this course are to: engage students in a critical analysis of current knowledge and understanding of the evolution, biology and ecology of the modern human species facilitate discussions on biological and other features of modern humans that have given rise to their success and dominance explore the many dimensions of the complex issues facing humans with particular reference to health and disease, availability of resources, environmental degradation and global climate change lead students in the creative process of crafting solutions for the major issues facing humans in the future LEARNING OUTCOMES: At the end of this course, students should be able to Evaluate the proposed unique characteristics of the human animal and assess the biological classification of the human species 285 Give a chronological account of the proposed biological evolution of modern humans from anthropoid ancestors and the associated stages of cultural and technological development Define biological success and dominance and analyse how selected characteristics have contributed to human success and dominance, e.g. anatomy, diversity, adaptability, the brain, language, intelligence, reproductive patterns, life history, social behaviour, social organisation, culture, technology and lifestyles. Describe the historical trends in human population growth and regional variation in demographic parameters, assess the influential factors contributing to such trends and outline projections and consequences for the future. Define the components of human well-being and discuss the status and consequences of inequity within populations and among nations. Assess the impacts of human populations, technology and lifestyles on ecosystems, food, water and material resources, and the effects of such impacts on human well-being including global climate change. Discuss and evaluate the proposed scenarios for the future of human populations, resources and the environment. Demonstrate skills in analysis, synthesis, evaluation of complex information; good oral and written communication; craft creative solutions using a variety of tools and strategic approaches; manage group interactions and work effectively in teams. COURSE ASSESSMENT: Assessment will be based on a student’s final mark from the components below. Component Final exam Coursework % Final Grade 50% 50% In-course tests 20% Tutorials, online activities 15% Practicals 15% Description 2-hour written exam with essay questions Broken down as follows: 2 tests (each 10%) based on multiple choice, true/false, short response questions or essay Performance in face-to-face and online discussions and activities Performance and reporting of 3 practical exercises ASSIGNMENTS: In-course tests (20%): 2 tests (each 10%) each 50 minutes duration including multiple choice, true/false, short response questions Tutorials, online activities (15%): Participation, performance and contributions in face to face tutorials and online discussions/activities Practicals (15%): Practical performance and reporting of 3 practical exercises. Please note guidelines and policies for attendance and plagiarism under ‘Additional Information’ and below. EVALUATION: Feedback on the course will be obtained informally from students on an ongoing basis by regular interactions and meetings among students, demonstrators, teaching assistants and the Course Coordinator in practicals and tutorials. 286 Formal feedback will be via election of Class Representatives who sit on the Departmental Student-Staff Liaison Committee meetings held twice during the semester. Class reps will channel both concerns and commendations to the meeting as guided by the Department’s Standard Operating Procedures. Formal evaluation of the entire course will be accomplished via a UWI Course Evaluation questionnaire administered anonymously and confidentially at the end of the semester. All feedback will be considered on an ongoing basis and corrective action or adjustments made or discussed with students promptly or incorporated the following year. TEACHING STRATEGIES: Contact hours 36 hours: Lectures: 24 h Tutorials: 6 h Practicals: 6 h (3x4 h = 12h practicals) Lectures: Lectures will provide valuable synthesis and evaluation of the growing body of available information, update current issues and events, and prioritise content relevant to course assessment. Lectures will generally be ‘flipped’ i.e. students are expected to use online resources to read and prepare in advance of the lecture so that the session can focus on questions, clarification, expansion and discussion. Practicals: Practicals will provide detailed exposure to selected topics and experiences and involve interactive activities and discussion to analyse, synthesise and evaluate complex issues and concepts. Participation and performance in the practicals will be assessed as well as reports based on the practical activities. Tutorials: Tutorials will cover course topics in a highly interactive format using a variety of collaborative active learning techniques. In addition to clarifying details of course content, specific transferrable skills will be addressed e.g. essay and report writing, critiquing and oral presentation. myelearning: The online teaching tool, myeLearning, will be used extensively during this course for communication among students and staff (email, discussions) official posting of important notices (coursework assessment notices, instructions, and in-course results) provision of course details, lecture notes, practical guides, tutorial briefings provision of recommended resource materials and links to resources on specific websites self-test quizzes, questionnaires, surveys interactive activities such as discussions, wikis, glossaries, blogs RESOURCES: Most resources are available myeLearning including Lecture presentations - include learning objectives, summaries, recommended readings Resources - links to papers, articles and websites with interactive resources and videos for those who prefer to learn using these modalities Tutorials - guidelines for tutorial activities and specific supporting resources Practicals - guidelines for practical activities and specific supporting resources Essential and Recommended texts are in the Reserve Section, UWI Main Library or via links in the course outline and myelearning Readings: Diamond, J. 1992. The Third Chimpanzee. Harper. Jones, S., R. Martin & D. Pilbeam. 1992. The Cambridge Encyclopaedia of Human Evolution. Cambridge UP. 287 Kormondy, E.J. & D.E. Brown. 1998. Fundamentals of Human Ecology. Prentice-Hall. Web links: Smithsonian Institution, National Museum of Natural History, Human Origins Programme. http://humanorigins.si.edu/ Human Biology. Wayne State University Press. http://www.bioone.org/loi/hbio UNEP. 2012. Global Environment Outlook 5 (GEO-5). UNEP. http://www.unep.org/geo/geo5.asp COURSE CALENDAR: WEEK 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 52. LECTURES 99. Course Introduction 100. What makes us human? 101. The human Primate 102. The evolution and origin of modern humans 103. The evolution and origin of modern humans 104. Anatomy and bipedalism 105. Culture, technology and lifestyles 106. Culture, technology and lifestyles 107. Genetic and cultural diversity and adaptation 108. The brain, language and intelligence 109. Sex, reproduction and life history 110. Social behaviour and organisation 111. Human populations 112. Human populations 113. Human well-being and inequity 114. Human health and disease 115. Human health and disease 116. Resources for the future 117. Resources for the future 118. Environmental degradation 119. Environmental degradation 120. Global climate change 121. Global climate change 122. The future of the human animal Course review PRACTICALS TUTORIALS Assignments Tutorial 1 Practical 1: Primate behaviour (Zoo) Tutorial 2 Practical 2: Human biological evolution Online activity 1 Tutorial 3 In-course Test 1 Practical 3: Lifestyles and health Tutorial 4 Tutorial 5 Tutorial 6 Online activity 2 In-course Test 2 ADDITIONAL INFORMATION: Refer to the Life Sciences Undergraduate Handbook for general guidelines for teaching, assessment, student conduct, essential skills, and support at http://sta.uwi.edu/fst/lifesciences/documents/handbook.pdf . Refer to the General Information and General Regulations in the Faculty Booklet available from http://sta.uwi.edu/resources/documents/facultybooklets/ScienceTechUndergrad.pdf . 288 Students are reminded that they must attend a minimum of 75% of the practical sessions and tutorials. Failure to do so will result in debarment from the final examination. As a general principle, medicals or other excuses may only excuse a student’s presence at an assigned time. Students must still complete the assigned work (make-up lab report or make-up test) in order to obtain the marks for that item of coursework. The student is responsible for liaising with the Course Coordinator or Teaching Assistants to ensure the assigned make-up is completed. Students are hereby informed that plagiarism is forbidden and all unsupervised coursework items must be accompanied by a Coursework Accountability Statement in order to be assessed. Specific items may require submission through Turnitin on myeLearning. Refer to ‘University Regulations on Plagiarism’ available from http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf HOW TO STUDY FOR THIS COURSE: Attendance is mandatory for lectures, tutorials and practicals. Prior preparation is strongly advised to able to fully participate in activities and obtain the full value of the sessions. Thorough use should be made of the resources provided and students are strongly advised to become familiar with them and start utilising them from the first week. Regular updates on course progress and materials are also highly recommended and you should be checking into myelearning on a frequent regular basis to review materials, assignments and activities. Students are encouraged to interact regularly with staff on their projects, even outside of the assigned tutorial times to ensure prompt, satisfactory solution of any problems and to monitor progress. Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 289 MAK: 18 February 2013 THE UNIVERSITY OF THE WEST INDIES, ST. AUGUSTINE FACULTY OF SCIENCE & TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE DOCUMENTATION COURSE CODE: BIOL3870 COURSE TITLE: Insect Biology NO. OF CREDITS: 3 LEVEL III PRE-REQUISITE: BIOL 2867 Physiology of Animals or BIOL2862 Animal Physiology ANTI-REQUISITE: BIOL2866 Entomology COURSE DESCRIPTION The first half of the course treats the unity of insects, i.e. those features that are common to all or many orders. The second half is an evolutionary survey of the insects, with some attention to arachnids, treating major orders and some families or superfamilies. In addition, one lecture is devoted to a more in-depth treatment of a selected group of insects or arachnids or a particular theme in arthropod biology. The basic teaching/learning approach is a traditional one of practical exercises followed by lectures and reading. Assessment is by means of reports on practical exercises, tests and an individualized species account. COURSE RATIONALE Insects are an extremely speciose and abundant group of organisms on land and in fresh water, accounting for about half of all known species of organisms. As such, they have enormous ecological impact, apart from the economic and medical importance of some groups. For a biologist with special attention to the whole-organism and population levels or organization, then, knowledge of the biology and diversity of insects is a key part of being educated. INSTRUCTOR INFORMATION Christopher K. Starr 290 Natural Sciences Bldg new wing Rm 225, phone ext 3096 or 303-9919 [email protected] Office hours: Most days from about 07:30 to about 18:30 LETTER TO THE STUDENT Dear Scholar, It is my distinct pleasure to welcome you to BIOL 3xxx – Insect Biology. I will not pretend to regard this subject with cool detachment, as it has been my unshakeable conviction for almost 60 years that these creatures are, quite simply, the most interesting feature of the known universe. Insects are ubiquitous and strikingly abundant in land and freshwater environments throughout the inhabitable world, where they have enormous ecological impact. It is my business to aid you in learning who they are, what they are like, and their major roles in ecosystems. By doing well in this course, you will arm yourself with an understanding of a critically important part of the biota. Sincerely, Christopher K. Starr Professor of Entomology CONTENT: As outlined in the course calendar GOALS/AIMS The course aims to provide a solid grounding in both the unity and diversity of the class Insecta, with some attention to the class Arachnida. Students who do well will have a good understanding of how insects are built and what distinguishes them from other animals. In addition, they will be familiar with major orders of insects and their ways of life. LEARNING OUTCOMES At the end of this course, students will be able to: 1. Characterize the global diversity of insects and discuss putative reasons for this diversity. 2. Outline the structure and nature of the insect cuticle and its importance in the success of insects. 3. Take a generalized adult insect apart and put it back together again. 4. Define and describe “metamerism” and “tagmosis” to any literate person in such a way as to convey a satisfying basic understanding. 5. Describe the main features of major organ systems in insects and their functioning, with emphasis on how these differ from our own organs. 6. Describe the basic structure of wings and how flight works. 7. Explain the concept of the “sensory world” and explain cogently how this differs between ourselves and a generalized adult diurnal insect. 8. Describe the three major forms of metamorphosis. 9. Hold forth on the developmental, ecological and evolutionary significance of the pupa. 10. Name a dozen orders of insects and outline the distinguishing physical and ecological features of each. 291 11. Name three groups of ectoparastic insects and compare them with respect to gross structure and way of life. 12. Name three orders of arachnids and outline the distinguishing physical and ecological features of each.. ASSIGNMENT The theory coursework, accounting for 10% of total marks, will be a species account in the style of those in D.H. Janzen’s (1983) Costa Rican Natural History. Species will be individually assigned to students by lot early in the semester, with the finished species account due four weeks later. COURSE ASSESSMENT Practical coursework Lab book ... 15% Two in-course lab tests ... 20% Theory coursework Assigned species account ... 15% Final theory exam ... 50% EVALUATION The Department of Life Sciences takes the quality control of its courses very seriously. Students who have comments on one or another aspect of a course are encouraged to communicate these to the instructors. More formal feedback is sought through the Staff-Student Liaison Committee, which meets twice per semester. This departmental committee comprises elected student representatives from each course in the given semester. In addition, the university conducts a confidential written evaluation of most courses and their instructors toward the end of each semester. TEACHING STRATEGIES The course comprises 18 lectures one-hour lectures, six one-hour tutorials and eight three-hour practical sessions. Tutorials are about collective problem solving with minimal lecture-style talking by the instructor. However, lectures always have a certain tutorial aspect, so that the instructor will often pose questions to members of the class and will expect answers. Students who prefer to sit passively in class without active involvement should not register for this course. Where a given topic arises both in a practical exercise and in lecture, every effort is made to treat it in lab first. That is, it is much preferred in lecture to draw attention to prior practical observations and try to make sense of them than to say what the student can expect to observe later in the lab or field. Students are expected to read widely in materials available in the university library, on the internet and on MyeLearning, as an extension of topics summarized in lecture. RESOURCES There is no dearth of suitable reading matter in the university library, on the internet and on MyeLearning. The library has several relevant textbooks of general entomology (QL461) and a good set of books on the ecology and behaviour of insects (mostly around QL495), as well as on particular groups (in the QL500- and QL600-ranges). In some cases, these are placed on reserve. 292 Practical exercises in the field and lab will make use of standard equipment and materials. In addition, we will have access to the department’s significant library of natural-history movies on DVD. The course manual is the 2006 edition of the in-house Neotropical Entomology: Study Guide for BIOL 2866. READINGS Ambrose, D.P. 2004. The Insects: Structure, Function and Biodiversity. Ludhiana: Kalyani 821 pp.[Sections 1 and 3 good for weeks 2-6, but disregard for later weeks.] Chapman, R.F. 1998. The Insects: Structure and Function. Cambridge: Cambridge Univ. Press 770 pp.[Very good for weeks 2-6, although treatment of many topics more detailed than you want.] Davies, R.G. 1988. Outlines of Entomology. London: Chapman &Hall 408 pp. [Good on all topics.] Elzinga, R.J. 2004.Fundamentals of Entomology. 6th ed. Upper Saddle River, New Jersey: Pearson-Prentice Hall 512 pp. [Good for all topics. Illustrations especially good.Earlier editions also useful.] Gillott, C. 2005. Entomology. 3rd ed. Dordrecht: Springer 831 pp.[Very good for first half of course, okay for second half.Some earlier editions on shelf.] Ross, H.H., C.A. Ross & J.R.P. Ross 1991.A Textbook of Entomology. 4th ed. Malabar, Florida: Krieger 666 pp. [Good for second half of course, but disregard for first-half topics. Earlier editions on shelf.] See also non-circulating encyclopedias of entomology on shelf at QL462.3. COURSE CALENDAR Note that Saturday is regarded as a school day at UWI, with class exercises sometimes scheduled for that day. Students with scruples against Saturday schoolwork should not register for this course. Week 1 2 3 Practical Morphology of a generalized adult insect Field trip to secondary lowland forest 4 5 Metamorphosis and larval growth rates Lecture/tutorial Introduction to the scope and limits of entomology The nature of insects and their success Tutorial: Estimating the global species richness of insects Structure and functions of the cuticle Metamerism, segmentation and tagmosis Assignment Lab report due Internal morphology and physiology Circulation and respiration Tutorial: The limits of growth Internal morphology and physiology Feeding, excretion and reproduction Flight Internal morphology and physiology Central nervous system and perception Tutorial: Sensory world of the honey bee Lab report due 293 Week 6 Practical Evolutionary survey: Primitively wingless orders, Odonata, orthopteroid orders 7 8 9 10 11 12 Evolutionary survey: hemipteroid orders Evolutionary survey: Coleoptera Evolutionary survey: Lepidoptera, Diptera, Siphonaptera Evolutionary survey: Hymenoptera, Arachnids Lecture/tutorial Growth and allometry Metamorphosis and the nature of the pupa Assignment Lab exam Collembola and Odonata Tutorial: Reading the cladogram of insect orders Orthopteroid orders Lab report due In-depth lecture: Our dear friend the termite (Isoptera) Hemiptera Tutorial: Ectoparasitic insects Assigned species account due Coleoptera Lepidoptera (moths and butterflies) Lab report due Diptera Tutorial: Shifting balances in metamorphosis Hymenoptera Arachnida Lab exam ADDITIONAL INFORMATION Attendance is not recorded at lectures and tutorials, but missing them is not a good idea. Attendance at practical exercises is mandatory, and the student should report any excusable absence promptly (in advance, if this is feasible). HOW TO STUDY FOR THIS COURSE It is a regrettable necessity that students must work in isolation during final exams and other tests. At all other times, collaborative work is warmly encouraged. There is much to be gained from discussion of topics and problems among classmates. 294 Lectures provide a necessary focus for studying, but they do not by any means provide the entirety of what should be learned. Rather, the student should take lecture material as a point of departure in extensive outside reading. This supplemental material deepens one’s understanding of principles treated rather summarily in lecture and provides illustrations of these principles. It is a good idea to bring your copy of the course manual not only to lab but to lecture as a matter of course. It includes summaries of all lectures and a number of illustrations and appendices to which reference will be made in lecture. Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 THE UNIVERSITY OF THE WEST INDIES ST. AUGUSTINE FACULTY OF SCIENCE AND TECHNOLOGY Proposal for introducing new course in the Department of Life Sciences Course code: BIOL 3960 Course Title: Environmental Microbiology 295 Number of Credits: 3 . Level: Undergraduate –Year III Pre-requisites: BIOL 2265 – Fundamentals of Microbiology (at least B-) BIOL 2164 - Principles of Molecular Biology BIOL 2165 - Genetics II BIOL 2360 - Biochemistry IIA Semester: 1 Offering Department: Department of Life Sciences, Faculty of Science and Technology, The University of the West Indies, St. Augustine Enrolment capacity: 45 Course description This course explores the diversity and function of microorganisms in the environment. Emphasis is placed on metabolic processes employed by microbes to transform organic and inorganic substances as part of bio-geochemical cycles. The role of microorganisms in pollution of water, soil and air is considered in addition to microbial processes used in environmental remediation and conservation. Conventional and molecular-based tools used for detecting, characterizing and monitoring microbes in the environment are also covered. The teaching and learning methods include lectures/tutorials, discussion sessions and labs. Assessment would be via coursework (50% comprising incourse tests, graded practical reports, class participation) and final semester exam (50%). Purpose of course/rationale BIOL3960 forms part of the proposed microbiology specialization in the biology degree in the Department of Life Sciences and it would serve as an elective in the ESST programme in the Faculty of Science and Technology, UWI, St. Augustine. It will be highly beneficial to students with specific interest in environmental microbiology and environmental science. The course is designed to introduce students to fundamental theoretical principles and develop key practical skills in environmental microbiology. Environmental microbiology is an important sub-discipline of microbiology and is critical to environmental management and sustainability. It also contributes significantly to environmental health and microbial biotechnology. Instructor Information BIOL 3960 will be managed by a course coordinator and will be taught by a team of experienced lecturers, supported by a Teaching Assistant/Instructor and laboratory demonstrators. Office hours for the coordinator and lecturers are posted on the faculty bulletin boards and are available from the Department of Life Sciences’ general office. Team members can also be contacted via e-mail. Name: Adesh Ramsubhag (Co-ordinator) 296 E-mail: Office location: Office hours: Phone: Name: E-mail: Office location: Office hours: Phone: Letter to students Welcome to BIOL 3960- Environmental Microbiology. This course provides an exciting opportunity to be introduced into the exciting field of environmental microbiology. It would provide a platform for developing a fundamental understanding of theoretical concepts and practical skills in exploring microbes in the environment and applying them in management of the environment. A major emphasis would be to develop critical thinking skills. Interactive participation in lectures, tutorials and practical sessions is essential. Please feel free to ask questions or offer alternative perspectives. You would also need to focus on personal attributes of team work and inter-personal skills required for group work, in addition to organizational skill and time management. I wish you the most interesting and intellectually stimulating experience in this course. Content: BIOL3960 covers the following content: 1. Overview of diversity and distribution of microorganisms in aquatic and terrestrial environments. a. Surface b. Shallow subsurface c. Deep sub-surface 2. Carbon and energy metabolism of environmental microorganisms in aerobic and anaerobic environments. a. Chemoheterotrophy b. Chemoautotrophy c. Photoheterotrophy d. Photoautotrophy 3. 4. 5. 6. 7. 8. Role of microbial metabolism on bio-geochemical cycles Survival mechanisms of microbes in extreme environments Microbial communication and interactions with higher organisms Pollution microbiology- water, soil and air. Traditional and new microbial indicators of pollution Detecting, enumerating and monitoring microorganisms in the environment a. Culture based methods b. Direct microscopic counts 297 c. Physiological and immunological methods d. Conventional nucleic acid and PCR based methods e. “OMICS” based approaches 9. Water, air and soil bioremediation 10. Microbial processes in water and wastewater treatment systems Course goal: The goal of the course is to expose students to general theoretical concepts and develop fundamental practical skills in environmental microbiology. General Objectives: Students completing the course will have: 1. A critical review of microbiological processes in the environment; 2. Fundamental practical skills in detecting, enumerating and monitoring environmental microorganisms. Learning objectives: Upon completing this course, students will be able to: - Summarize the diversity and distribution of major groups of microorganisms in the environments; - Explain the carbon and energy metabolic processes of environmental microorganisms; - Explain the role of microbial metabolism on bio-geochemical cycles; - Compare the survival mechanisms of microorganisms surviving in extreme environments; - Explain microbial communication and interactions strategies; - Assess the role of microorganisms in pollution of water, soil and air; - Critique the traditional and new microbial indicator-based methods of assessing faecal pollution; - Evaluate the various ways of detecting, enumerating and monitoring microorganisms in the environment a. Culture based methods; b. Direct microscopic counts; c. Physiological and immunological methods; d. Conventional nucleic acid and PCR based methods; e. “OMICS” based approaches; - Devise bioremediation strategies for contaminated water, air and soil; - Explain the microbial processes in water and wastewater treatment systems. Mode of Delivery: Lectures -18 hours: Tutorials -12 hours: Practical exercises: Didactic; interactive Interactive; mind maps; problem-solving Five 3-hr sessions 298 ASSIGNMENTS: Coursework for BIOL 3960 will be assigned as follows: 1. In-course Test-20% Two in-course tests worth 10% each in week 5 and 10. 2. Practical reports-20% Students are required to complete five laboratory reports based on practical exercises worth 4% each. 3. Journal paper discussion and reports - 5% Students will be given one journal article on relevant environmental microbiology research to study. The paper will be discussed in class and students will be required to submit summary reports on each paper. 4. Class participation- 5% Student participation in tutorial sessions will be monitored by the tutor and a grade assigned at the end of the semester. Participation shall include more than just showing up at tutorial – some evidence of an active role in the tutorial discussion would be required to obtain the full 5% for participation. At the beginning of the semester, the students in each tutorial would engage in developing the exact assessment criteria for this assignment, and the student-developed criteria will be used to determine each student’s participation grade. Cheating, including plagiarism, would not be tolerated. Incidents of cheating would be dealt with according to UWI’s rules and regulations including: - Examination Regulations for First Degrees, Associate Degrees, Diplomas and Certificates. Section (B) Cheating. Regulations 96–102; http://sta.uwi.edu/resources/documents/Exam_and_GPA_regulations.pdf. - University Regulations on Plagiarism (First Degrees, Diplomas and Certificates). http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf. Assessment: Course Work: 50% - Incourse tests (Two written tests X 10%) - Lab reports - Journal paper discussion and reports - Tutorial Attendance and participation 20% 20% 5% 5% Final Semester Examination: 50% - One 2-hour comprehensive written paper comprising of o 4 short answer questions worth 10% each 40% 299 TOTAL o 2 essay-type question worth 30% each All questions in this paper shall be compulsory 60% 100% EVALUATION: BIOL3960 will be evaluated in two ways – (a) through the offices of the Class Representative and the Life Sciences Student-Staff Liaison Committee, and (b) an end of semester course evaluation survey. The class will elect four class representatives (one per lab stream), whose role is to act as a mediator between the Life Sciences academic staff and the students in the class. The representatives will attend Liaison Committee meetings (held at least twice per semester), where they will present feedback on the course to the Department for action. The UWI performs a course evaluation survey at the end of every semester, and this information will also be used for overall assessment of the course and guide possible actions for improvement in subsequent semesters. TEACHING STRATEGIES: A combination of teaching strategies will be adopted in BIOL3960. The primary teaching strategy will be based on the face to face classroom lectures and discussions. Videos of lecture presentations would be made available to students prior to the lecture session via podcast media. Students are advised to view these videos before the lecture so that more emphasis can be placed on discussions and answering questions, thereby facilitating deep learning. This would also afford the time to have assessment exercises on “writing across the curriculum”. MyeLearning will be utilized throughout the course as a means to provide access to course materials such as Powerpoint presentation files, animations, weblinks, lessons and quizzes. This medium would also be used as a portal for student-lecturer communications and the dissemination of coursework feedback. A total of 12 tutorial sessions would be given for the semester. These would be small group sessions which would be conducted by instructors or tutors, who would normally by senior postgraduate students in Life Sciences. Students must attend tutorial sessions. Tutorials sheets will be assigned prior to each session via myeLearning, and students are expected to attempt the solutions before coming to tutorial. The goal of the tutorial session is to give students a more hands-on experience with the course material and easier access to course instructors. Students will be expected to ask and answer questions on material that is unclear, propose solutions to questions on the tutorial sheet and to generally participate fully in the tutorial activities. Tutors will not merely be going through the answers to the tutorial questions in these sessions. The practical component of the course would comprise of 5 laboratory exercises that include laboratory management systems, general & specific methodologies applied to environmental microbiology, and applications to water, soil and air. The exercises would be based on conventional microbiological techniques and molecular methods such as singleplex and multiplex conventional PCR and qPCR; metagenomics applications for investigating microbial communities; and molecular-based biosensors. 300 In order to pass the course, you must gain an overall passing mark of 50%. Any student who misses more than 25% of practical or tutorial sessions without a medical or other valid excuse can be debarred from writing the final exam. RESOURCES: Text books: Essential: Ian L. Pepper, Charles P. Gerba, Terry J. Gentry (Eds) Environmental Microbiology, Third Edition Hardcover, 2014, Academic Press, San Diego, USA Recommended: Eugene L. Madsen, Environmental Microbiology: From Genomes to Biogeochemistry, 2015, Wiley-Blackwell, USA Ian L. Pepper (Author), Charles P. Gerba (Author), Jeffrey W. Brendecke, Environmental Microbiology, Second Edition: A Laboratory Manual, 2004, Academic Press, San Diego, USA Lab coat: - Each student should have suitable Lab coat to use for practical exercises. Students not having lab coats will not be permitted in the lab. Standard microbiology laboratory, equipment, reagents and kits Microbiology technician and demonstrators Course calendar: Week Lecture/Tutorial/lab 1 Introduction to course Diversity and distribution of microorganisms in aquatic and terrestrial environments 2 Tutorial Microorganisms in the environment: - Surface - Shallow subsurface - Deep sub-surface Photoheterotrophy vs photoautotrophy 3 Tutorial Chemoheterotrophy vs chemoautotrophy Microorganisms and bio-geochemical cycles Tutorial 301 Week Lecture/Tutorial/lab Lab: Microbiology Lab, Management of systems (Data management, Lab safety, Certification, Quality control and standards) 4 Microorganisms and bio-geochemical cycles Survival mechanisms of microbes in extreme environments Tutorial 5 Microbial communication and interactions with higher organisms Tutorial Lab: General environmental microbiology laboratory methods Advanced quantitative plating methods; Isolation and quantitation of phages; Incourse test- 5% (online) 6 Pollution microbiology- water, soil and air. Tutorial 7 Pollution microbiology- water, soil and air. Traditional and new microbial indicators of pollution Tutorial Lab: Water microbiology 8 Detecting, enumerating and monitoring microorganisms in the environment Tutorial 9 10 11 Journal report due Detecting, enumerating and monitoring microorganisms in the environment Tutorial Lab: Soil Microbiology Water, air and soil bioremediation Tutorial Incourse test 2 (10%) Water, air and soil bioremediation 302 Week Lecture/Tutorial/lab Tutorial Lab: Air microbiology Microbial processes in water and wastewater treatment systems Tutorial 12 31 Review Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 303 THE UNIVERSITY OF THE WEST INDIES, ST. AUGUSTINE FACULTY OF SCIENCE & TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE DESCRIPTION Course Code: BIOL3961 Course Title: PRINCIPLES OF MEDICAL MICROBIOLOGY No. of credits: 3 Level: III, Sem I Prerequisites: BIOL2265-Fundamentals of Microbiology (minimum grade B-) BIOL 2164 - Principles of Molecular Biology BIOL 2165 - Genetics II BIOL 2360 - Biochemistry IIA Enrollment Capacity: 45 maximum: Course Description: BIOL3XXX- Principles Medical Microbiology gives students a detailed insight into the principles and techniques of microbiology applied to human medicine. It covers medically important bacteria, viruses, fungi and parasites. Emphasis is placed on classification, detection and diagnosis of microbial pathogens and parasites in addition to their mechanisms and clinical manifestation. Students would also gain an understanding of epidemiological factors that contribute to human infectious disease and be introduced to the uses and challenges of antimicrobial and anti-parasitic agents for managing microbial diseases. The teaching and learning methods include lectures/tutorials and laboratory sessions. Assessment would be via coursework (50% comprising incourse tests, graded practical reports, class participation) and final semester exam (50%). Course Rationale BIOLXXXX forms part of the proposed microbiology specialization in the biology degree in the Department of Life Sciences. It will be highly beneficial to students with specific interest in medical microbiology. The course is designed to introduce students to fundamental theoretical principles and develop key practical skills in medical microbiology. There is a current necessity for skill development in this field at the undergraduate level which will provide students with opportunities for employment in medical laboratories. The curse will also enable students to prepare for higher studies in Microbiology, Epidemiology, Biotechnology and Biochemistry. Instructor Information BIOL XXXX will be managed by a course coordinator and would be taught be a team of experienced lecturers, supported by a Teaching Assistant/Instructor and laboratory 304 demonstrators. Office hours for the coordinator and lecturers are posted on the faculty bulletin boards and are available from the Department of Life Sciences’ general office. Team members can also be contacted via e-mail. Name of instructor(s): Office address and phone: Email address: Office hours: XXXXXX XXXXXX XXXXXX XXXXXX Letter to Students Welcome to BIOL XXXX- Principles of Medical Microbiology. This course provides the basic knowledge about microbial pathogens and parasites as well as information and skills for applications to human health and disease management. The course is taught through a series of lectures/tutorials, and laboratory sessions. It is quite extensive and students must make a sincere effort to do well by full participation and involvement in course activities. It is important to attend lectures. Please make use of supplementary information provided to strengthen foundation and enhance understanding of material covered. Content An introduction to medical microbiology including general properties, nomenclature, classification and pathogenicity of bacteria, fungi, viruses and parasites. Systematic study of medically important bacteria, fungi and parasites. An overview of medically important viruses. Principles of Clinical Bacteriology, Public Health Bacteriology, Mycobacteriology, Hospital Acquired Infections; Microbial toxins and enzymes in disease; Innate and adaptive immunity and microbial infections; Laboratory diagnosis of bacterial, viral and fungal diseases and parasitic infestations. Antimicrobial agents and antibiotic resistance. Principles of epidemiology of human infectious diseases. Goals/Aims This course aims to: present students with the background on human disease problems caused by microbial pathogens and parasites. introduce students to scientific developments and information on human microbial pathogens and parasites, and their management. expose and update students to the constantly evolving procedures and techniques of medical microbiology Learning Outcomes At the end of this course students will be able to: summarize the characteristic features including morphology, physiology, genetics, modes of transmission and diseases of medically important bacteria, fungi, viruses and parasites; 305 explain the mechanisms of pathogenesis of microbial pathogens; explain the role of toxins and enzymes in infectious disease; discuss host responses and related epidemiology to pathogenic infections and parasitic infestations; distinguish the different antimicrobials and antiparasitic agents and explain the development and spread of resistance to these agents; compare and contrast the different approaches for diagnosing infectious diseases and parasites; apply microscopic, biochemical, serological and molecular based tools to diagnose common infectious diseases and parasites; demonstrate skills in critical thinking, laboratory experimentation, teamwork and communication; Assignments: Coursework for BIOL 3XXX will be assigned as follows: 1. In-course Test-20% Two in-course tests worth 10% each in weeks 6 and 11. 2. Practical reports-20% Students are required to complete five laboratory reports based on practical exercises worth 4% each. 3. Journal paper discussion and reports - 5% Students will be given one journal article on relevant medical microbiology research to study. The paper will be discussed in class and students will be required to submit summary reports on each paper. 4. Class participation- 5% Student participation in tutorial sessions will be monitored by the tutor and a grade assigned at the end of the semester. Participation shall include more than just showing up at tutorial – some evidence of an active role in the tutorial discussion would be required to obtain the full 5% for participation. At the beginning of the semester, the students in each tutorial would engage in developing the exact assessment criteria for this assignment, and the student-developed criteria will be used to determine each student’s participation grade. Course Assessment Coursework: 50% - Two incourse tests (2 X 10%) - Lab reports - Journal paper discussion and reports Attendance and participation 20% 20% 5% Tutorial 5% Final exam – 50% Evaluation 306 The elected Class Representative and/or Deputy will attend meetings with the course teacher(s) organized at every 4th week, and present feedback from the students attending that course or stream. This feedback is normally provided both orally and in written form for transmission to the lecturer. Apart from that the representatives will be attending the Liaison Committee meeting, and give their feedbacks to the committee. Students may comment on any aspect of the course or facilities. Students will be encouraged to submit their feedback (oral/written) during tutorials directly to the course teacher and appropriate actions will be taken by the teacher then and there. Results of in-course tests, quiz and other course assignments will be analyzed and presented in the class. This will help students to check their progress constantly and also helps the instructor to identify the weak areas and thereby could alert and advise students individually to alter their approach of study and completing the work. The final reflective feedback and comments about the entire course and teaching will be collected on the last day of the course. This will be saved for analysis and utilized as a base for improvement for the next offering in the following year. Teaching Strategies Contact hours (36 hours): Lectures+Tutorials: 26 h Practicals: 20 h Students are expected to learn through both directed and self-directed study. Formal instruction for this course would comprise of 2 Lectures with inbuilt tutorials per week and 5 practicals involving laboratory exercises. Additionally, instructional videos, would be utilized to support the teaching. Formal lectures and tutorials will provide introduction to specific topics and allow for discussion in an interactive setting. Case studies/journal papers discussion will be carried out in small group settings. Course materials and readings will be available online in advance so that discussion and critiquing can take place within the assigned class time. This course is fully mye-learning supported, and many other resources would be readily available for the students. Resources Recommended reading: - Medical Microbiology, 7th Ed. (2012) Murray, Rosenthal, and Pfaller, Elsevier-Mosby, ISBN: 978-0-323-08692-9 - Medical Microbiology, 4th Ed. (2004) Ryan and Ray (Eds.), McGraw-Hill, ISBN: 0-83858529-9. 307 - Medical Microbiology, A Guide to Microbial Infections: Pathogenesis, Immunity, Laboratory Diagnosis, and Control, 17th Ed. (2007) Greenwood, Slack, and Peutherer (Eds.), Churchill Livingstone; ISBN: 978-0443102097 - Medical Microbiology, Anjali Aggarwal (2010) International Scientific Publishing Co., New Delhi, India. ISBN: 81-8293-034-0. Lab coat: Each student should have suitable Lab coat to use for practical exercises. Students not having lab coats will not be permitted in the lab. Standard microbiology laboratory, equipment, reagents and kits Microbiology technician and demonstrators COURSE CALENDAR Week Wk1 Introduction Lecture + Tutorial Lab Bacterial infection, pathogenesis and host responses Wk2 Gram positive bacteria Wk3 Gram negative bacteria- Enterobacteriaceae Culturing and biochemical identification of bacteria pathogens Antibiotic sensitivity testing Wk4 Actinobacteria-Mycobacterium and Corynebacterium Spirochetes, Rickettsiae, Chlamydia Wk5 Mycoplasmas Nosocomial infections Wk6 Molecular and serological identification of bacterial pathogens Public health microbiology Incourse test Wk7 Principles of epidemiology of infectious human Laboratory diagnosis of diseases viral diseases Antimicrobial chemotherapy, drug resistance Wk8 Overview of important human viral agents 308 Wk9 Mycology- Yeasts and Moulds Laboratory diagnosis of fungal diseases Journal report due Wk10 Mycology- Yeasts and Moulds Wk11 Parasitology- Protozoa and Helminths Identification of parasites Incourse test Wk12 Parasitology- Protozoa and Helminths Wk13 Review Additional information: Refer to the Life Sciences Undergraduate Handbook for general guidelines for teaching, assessment, student conduct, essential skills, and support at http://sta.uwi.edu/fsa/lifesciences/documents/handbook.pdf . Refer to the General Information and General Regulations in the Faculty Booklet available from http://sta.uwi.edu/fsa/documents/UGFacultyBooklet.pdf . Students are reminded that they must attend a minimum of 75% of the practical sessions and tutorials. Failure to do so will result in debarment from the final examination. As a general principle, medicals or other excuses may only excuse a student’s presence at an assigned time. Students must still complete the assigned work (make-up lab report or make-up test) in order to obtain the marks for that item of coursework. The student is responsible for liaising with the Course Coordinator or Teaching Assistants to ensure the assigned make-up is completed. Students are hereby informed that plagiarism is forbidden and all unsupervised coursework items must be accompanied by a Coursework Accountability Statement in order to be assessed. Specific items may require submission through Turnitin on myeLearning. Refer to ‘University Regulations on Plagiarism’ available from http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf How to study for this course: Attendance is mandatory for lectures, tutorials and practicals. Prior preparation is strongly advised to able to fully participate in activities and obtain the full value of the sessions. Thorough use should be made of the resources provided and students are strongly advised to become familiar with them and start utilising them from the first week. Regular updates on course progress and materials are also highly recommended and you should be checking into myelearning on a frequent regular basis to review materials, assignments and activities. Students are encouraged to interact regularly with staff, even outside of the assigned tutorial times to ensure prompt, satisfactory solution of any problems and to monitor progress. Good group management is essential and requires good communication and interpersonal skills. 309 Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 THE UNIVERSITY OF THE WEST INDIES ST AUGUSTINE CAMPUS FACULTY OF SCIENCE & TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE DOCUMENTATION 2015/16 TITLE: Aquaculture COURSE CODE: BIOL3970 LEVEL: 3 SEMESTER: 1 CREDITS: 3 PRE-REQUISITES: BIOL1262 (Living Organisms 1) and BIOL1263 (Living Organisms 2) and BIOL2464 (Fundamentals of Ecology) 310 COURSE DESCRIPTION In Aquaculture you will be exposed to basic knowledge in the related fields of culturing fish in both the marine and brackish-water environments. You will learn about the various techniques and the exciting field of aquaculture – which is currently the fastest growing food-production system in the world. The course covers major trends in aquaculture practices, human and environmental influences on productivity and sustainability and traditional and modern strategies for managing aquaculture. Emphasis will be placed on tropical culture species. Students are expected to have a basic foundation in biology. In addition to providing a foundation of theoretical knowledge, this course will emphasise practical skills and expose students to field and laboratory approaches for studying aquaculture systems. It is an interactive ‘hands-on’ course where students are expected to prepare, participate and perform in an active way to engage with the content in a variety of ways. Assessment is designed to encourage students to work continuously with the course materials, explore and critically analyse research in this rapidly developing field. COURSE RATIONALE This is a core course for the Marine Biology specialisation, for the BSc. Biology degree in the Department of Life Sciences, and is an essential area in environmental sciences, environmental biology and environmental management. It also broadens the scope of studies in biology, plant biology and zoology. Aquaculture is a critical discipline in today’s world, given the importance of the fish and the seafood industry as a source of animal protein in the human diet. It is also the basis of a multi-billion dollar industry. 311 INSTRUCTOR INFORMATION This course will be taught by two lecturers assisted by a teaching assistant and a few demonstrators. Further logistical support will be provided by laboratory and field support staff. Office hours are posted on departmental notice boards and the main Life Sciences office. First examiner and course co-ordinator Dr Dawn A. T. Phillip Second examiner and lecturer Prof. Indar W. Ramnarine Principal lecturer Ms Rhea KingJulien Rm 223, Department of Life Sciences 662-2002, ext. 82208 [email protected] (preferred method of contact) Dean’s Office, 2nd floor, Chemistry 662-2002, ext. 84484 [email protected] (preferred method of contact) [email protected] (preferred method of contact) LETTER TO THE STUDENT Welcome to this level 3 course in aquaculture. This course forms part of the Specialisation in Marine Biology. The aim of this handout is to provide you with an outline of the course as well as related information with respect to course objectives and methods of assessment. Having completed BIOL1262 (Living Organisms 1) and BIOL1263 (Living Organisms 2) which are pre-requisite for this course, you should now be familiar with the diversity and biology of aquatic reptiles, crustaceans, echinoderms, molluscs and fishes. In this course (BIOL3970), students will be exposed to the broad and exciting field of aquaculture. In the first five weeks of lectures the general principles of aquaculture will be covered. This is followed by the biology and culture of several important species of tropical fishes, crustaceans, molluscs, plants and reptiles. An extensive reading list and important web sites are provided, and candidates are expected to READ around the topics covered in lectures. CONTENT Topics will include: a review of the history, status and future of global aquaculture; physical and chemical properties of water; water budgets and the general principles of aquaculture: choice of site, choice of species; choice of system. Lectures will also cover the different system designs for aquaculture, as well as the ornamental fish trade. Applications to local production in Trinidad and Tobago and the Caribbean will be discussed. Finally, the environmental aspects and impacts of aquaculture will be described. GOALS/AIMS This course aims to Use aquaculture resources as the focus to expand students’ knowledge of the range of aquatic animals that may be used as culture species. Develop students’ competence in basic methods and approaches for aquaculture farm and hatchery designs. Develop students’ awareness through lectures and practical sessions on how to develop and effectively manage an aquaculture farm or project. Introduce the range of aquaculture options highlighting their advantages and disadvantages. COURSE OBJECTIVES The general objectives of the course are to: provide course materials, activities and assessments using both face-to-face and online modalities 312 facilitate opportunities for students to engage with general concepts and specific examples to develop a comprehensive understanding of aquaculture allow students to observe, analyse and recognise a variety of aquaculture methods and practices allow students to analyse and evaluate the environmental impacts of aquaculture practices guide students in critically analysing and exploring questions in aquaculture facilitate discussions on the management of aquaculture in Trinidad and Tobago LEARNING OUTCOMES Upon successful completion of this course, the student will be able to: 1. describe the history and status of aquaculture, including the culture species and countries 2. identify the important water quality parameters and water budget for aquaculture production 3. explain the various criteria that are used in choosing a site for aquaculture, and the characteristics of extensive, semi-intensive, intensive and hyper-intensive aquaculture systems, and differentiate between static and re-circulating systems 4. explain the factors used in determining site selection and hatchery design, spawning and incubation techniques, water quality management and general hatchery management techniques and record keeping 5. describe the methods applied in pond design and construction, including irrigation and drainage structures; the pond-conditioning process; lime and fertiliser application rates; and pest and water quality management 6. analyse the importance of proteins, lipids, carbohydrates, vitamins and minerals in fish nutrition, and determine the nutrient requirements of fishes, fish feed formulation and manufacture 7. describe the major infectious and non-infectious diseases of fishes, including the important parasites that infect fishes and the subsequent losses that are incurred 8. explain polyculture systems, including their advantages and disadvantages 9. design and explain the operations of aquaponic systems, including their advantages and disadvantages 10. differentiate local areas (in Trinidad and Tobago) and their suitability for aquaculture 11. explain and evaluate the environmental impacts of aquaculture; both positive and negative 12. accurately identify species using species identification keys 13. sample aquaculture farms to collect biological data, particularly length, weight, sex and stage of maturity 14. progress to advanced studies in aquaculture or related professions. 313 TEACHING METHODOLOGY ASSIGNMENTS Laboratory exercises in weeks 4, 6, 8 and 10 (10%): assessment of practical skills, student performance during laboratory sessions, and written practical reports In-course theory quiz (10%): a single quiz is scheduled for week 6 Group project due in week 11 (30%): individual contribution to the project report (5%); individual participation during discussion sessions (5%); final project report (20%) Final theory examination (50%) COURSE ASSESSMENT The final mark for this course comprises the following Theory examination: one 2-hour written examination (50%) In-course theory exam (10%) In-course practical exercises and group project (40%) An in-course theory exercise worth 10% is due as in the course schedule. It may include a variety of types of questions: multiple choice, true/false statements and short paragraph questions. Any material covered in lectures, tutorials or practical sessions may be tested. Absence from any scheduled assessment must be accompanied by either a medical submitted to the Health Service Unit and copied to the main Life Sciences office, or a written excuse or explanation submitted to Dr Dawn A. T. Phillip or the TA within 7 days of the test. Students must complete a make-up exercise otherwise they will receive 0% for their in-course theory. Students are reminded that they are required to attend at least 75% of the lectures, practical exercises and tutorials. Failure to do so will result in the candidate being debarred from the final examinations. Any absences must be supported by a written excuse or medical certificate and submitted within 7 days of the missed session. In order to pass this course, students must gain a composite mark of at least 50%. Any make-up coursework assessment may be in the form of an oral examination or essay. EVALUATION Feedback on the course will be obtained informally from students on an on-going basis by regular interactions and meetings among students, demonstrators, teaching assistants and the course coordinator in lectures, tutorials and laboratory sessions. Formal feedback will be via elected class representatives who sit on the departmental Student-Staff Liaison Committee meetings held twice during the semester. Class representatives will channel both concerns and commendations to the meeting, as guided by the department’s standard operating procedures. Formal evaluation of the entire course will be accomplished via a UWI course evaluation questionnaire administered anonymously and confidentially at the end of the semester. All feedback will be considered on an on-going basis, and corrective actions or adjustments made or discussed with students promptly or incorporated the following year. TEACHING STRATEGIES Contact time will be 36 credit hours, comprising Lectures: 24 credit hours Tutorials: 4 credit hours 314 Practical exercises: 8 credit hours This course will be taught through a mixture of lectures, practical exercises and tutorials. There will also be guest lectures (on listed topics) by experts from the government and private sector. Lectures and tutorials will provide the conceptual and factual basis for specific topics and allow for discussions in an interactive setting. Course materials and readings will be available online in advance to allow students to be better prepared to engage the lecturers in discussion during class time. RESOURCES READING LIST Topic 1 Avault (1996) Fundamentals of Aquaculture Chapter 1. Getting started Chapter 2. Aquatic food production and people FAO (2010) The State of World Fisheries and Aquaculture (www.fao.org) Part 1: World review of fisheries and aquaculture Topic 2 Stickney (1976) Principles of warmwater aquaculture Chapter 2. Water systems Avault (1996) Fundamentals of Aquaculture Chapter 6. Water requirements Boyd (1988) Water quality management for pond fish culture Chapter 2. Water quality. Chapter 10. Hydrology of ponds. Topic 3 Avault (1996) Fundamentals of Aquaculture Chapter 3. Which species to culture Chapter 5. Site selection and culture systems. Stickney (1976) Principles of warmwater aquaculture Chapter 1. Introduction Topic 4 Piper et al. (1989) Fish Hatchery Management Avault (1996) Fundamentals of Aquaculture Chapter 13. Seed production and stock improvement. Stickney (1976) Chapter 6. Reproduction, selective breeding and genetics. Topic 5 Stickney (1976) Chapter 2. Water systems. Bardach et al. (1972) Aquaculture Appendix: Pond siting and construction Avault (1996) Chapter 5. Site selection and culture systems Boyd (1988) Chapter 2. Water quality Chapter 3. Fertilisation Chapter 4. Liming Chapter 5. Dynamics of dissolved oxygen 315 Avault (1996) Chapter 7. Water management Chapter 8. Fertilisation, liming and pond muds Stickney (1976) Chapter 3. Non-conservative aspects of water quality Chapter 4. Conservative parameters of water quality Topic 6 Lovell (1989) Nutrition and feeding of fishes. Avault (1996) Chapter 9. Feeding and nutrition Stickney (1976) Chapter 5. Feeds, nutrition and growth. Topic 7 Kabata (1985) Parasites and diseases of fish cultures in the tropics. Noga (1996) Fish disease: Diagnosis and treatment. Stickney (1976) Chapter 7. Disease and parasitism Avault (1996) Chapter 10. Maintaining health of a culture species. Topic 8 Bardach xi. (1972) Chapter 18. Culture of tilapias. Muir et al. (1982) Recent advances in Aquaculture Chapter 5. Intensive farming of tilapia Beveridge et al. (2000) Tilapias: Biology and exploitation. Costa-Pierce and Rakocy (2000) Tilapia farming in the Americas, vols 1 & 2 Rakocy and Ramnarine (1995) Tilapia farming methods Ramnarine and Ramnarine (1999) Recent developments in tilapia culture with applications for the Caribbean Bardach et al. (1972) Chapter 32. Shrimp culture. Nash (1991) Production of aquatic animals Chapter 2. Marine Penaeid Shrimp McVey (1983) Handbook of Mariculture Section II Nash (1991) Production of aquatic animals Chapter 3. Freshwater prawns New (1988) Freshwater prawn culture: a review Lum Kong and Ramnarine (1988) Biology of the river conch. Nash (1991) Chapter 7. Oyster culture. Chapter 8. Production of mussels. Bardach (1972) Chapter 36. Oyster culture. Ramnarine (1989) Cascadu farming – A manual for the culture of Hoplosternum littorale Ramnarine (1990) The biology of the cascadu, Hoplosternum littorale Ramnarine (1994) Larval development and growth of the cascadu Hoplosternum littorale Ramnarine (1994) A field trial of the semi-intensive culture of the cascadu Hoplosternum littorale Ramnarine (1995) Induction of nest building and spawning in the cascadu 316 Ramnarine (2001) Hatching trials with eggs of Hoplosternum littorale Topic 9 Bardach (1972) Chapter 3. Chinese carp culture. Little and Muir (1987) Integrated warm water aquaculture Topic 10 Rakocy (1997) Integrating tilapia culture with vegetable hydroponics in recirculating systems. Topic 11 The potential for an aquaculture industry in Trinidad and Tobago: Kenny (1981) Availability of water and land resources. Bruce (1981) Local species with potential for aquaculture in T&T Ramnarine (1996) A brief history of aquaculture in Trinidad and Tobago and the prospects for the development of an aquaculture industry Ramnarine and Ramnarine (1999) Aquaculture prospects in Trinidad and Tobago. 317 COURSE SCHEDULE Week Lectures Date Tuesdays, 10 am–noon, TLC TR5 1. 1Sep 2 8Sep 1. Review of the history, status and future of global aquaculture 2. Trends in global aquaculture production NO TEACHING 3 3. Physical and chemical properties of water; water 15Sep budgets 4. General principles of aquaculture: site, species, systems 4 5. Hatchery design. 22Sep 6. Hatchery operation and management 7. Farm design and pond construction 5 8. Pond and water quality management 29Sep 9. Fish nutrition 6 10. Fish-feed technology and fish energetics. 6Oct 11. Major fish diseases, control methods and vaccines 7 12. Tutorial 13Oct 13. Biology and culture of shrimp 14. Biology and culture of lobsters. 8 15. Biology and culture of prawns 20Oct 16. Biology and culture of oysters and mussels 9 27Oct 10 3Nov Practical sessions (Fridays 1–6pm, LS 1 &2) and assessment due dates 17. Biology and culture of snappers and groupers 18. Polyculture and integrated aquaculture 19. Principles of aquaponics. 20. Aquaponics systems 21. Biology and culture of turtles 11 22. Biology and culture of crocodilians 10Nov 23. The ornamental fish trade 12 24. Aquaculture prospects in Trinidad and Tobago. 17Nov 25. Environmental aspects of aquaculturure 13 26. Tutorial 24Nov 27. Tutorial 28. Tutorial Practical Practical In-course quiz Practical Practical Group project due ADDITIONAL INFORMATION Refer to the Department of Life Sciences’ Undergraduate Handbook for general guidelines for teaching, assessment, student conduct, essential skills, and support at http://sta.uwi.edu/fst/lifesciences/documents/handbook.pdf . Refer to the general information and general regulations in the Faculty Booklet available from http://sta.uwi.edu/resources/documents/facultybooklets/ScienceTechUndergrad.pdf . Attendance: Students are reminded that they must attend and report satisfactorily on a minimum of 75% of the practical sessions and tutorials. Failure to do so will result in debarment from the final 318 examination (examination regulations, section II (18) http://sta.uwi.edu/resources/documents/Exam_and_GPA_regulations.pdf). Medicals, excuses: Any student who misses a class, test or assignment is advised to consult immediately in person or by email with the TA or course coordinator regarding reasons for their absence and make-up options. Absence must be explained in writing within 7 days of the missed session by either a written/emailed excuse sent to the TA or course coordinator, and/or a medical certificate submitted to the Health Service Unit and copied to the Departmental Secretary, Life Sciences. Makeups: As a general principle, medicals or other excuses may only excuse a student’s presence at an assigned time. Students must still complete the assigned work (make-up lab report or make-up test) in order to obtain the marks for that item of coursework. The student is responsible for liaising with the course coordinator or teaching assistant to ensure the assigned make-up is completed. Any student who was inexcusably absent from a practical or test or does not submit a practical report (initial or make-up) will receive 0% for that exercise. Note that a field trip cannot be repeated for a make-up so you should make every effort to attend field trips. Late submission: A penalty at 10% of mark per day late will be applied unless formal approval for late submission is given by the TA or course coordinator using the guidelines above for medicals, excuses. Assignment submission: Hard copy reports must be submitted into the labelled deposit box for your bench/demonstrator in the outer Zoology Office and the student list must be signed. Submissions anywhere else will not be assessed. Reports will be returned at a time arranged by the TA or demonstrator. We are not responsible for reports left uncollected or submitted inexcusably late. Soft copy reports or other assignments must be submitted through myeLearning as directed for each assignment. Academic Dishonesty: Academic dishonesty, which includes plagiarism and cheating, is an extremely serious academic offense and carries penalties varying from failure in an assignment to possible suspension from the university. Students are hereby informed that plagiarism is forbidden and all unsupervised coursework items must be accompanied by a completed plagiarism declaration form in order to be assessed. Specific items may require submission through Turnitin on myeLearning. Refer to the ‘University Regulations on Plagiarism’ available from http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf Safety in the lab and field: Medical data and emergency contact forms must be completed in the first lab session when safety briefings will be given. Standard laboratory protocols for conduct and dress code are in effect once you are present in a Department of Life Sciences laboratory (Undergraduate Student Handbook, section 8). Dress code includes mandatory lab coats, adequate clothing to protect the body, and closed shoes. NO sandals, slippers or open-toed shoes will be allowed in the lab or field. Marks will be deducted for non-adherence to these protocols. Field work: Details are given in section 8 of the Undergraduate Student Handbook http://sta.uwi.edu/fst/lifesciences/documents/handbook.pdf . Field trips are specified on the attached course calendar so you can be adequately prepared. You will not be allowed on the field trip if a medical data form has not been submitted. If you have a disability and have not disclosed the nature of your disability and the support you need, you are invited to contact the Academic Support/Disabilities Liaison Unit (ASDLU) of The University of the West Indies (UWI), St. Augustine Campus. Phone: 662-2002 extension 83866, 83921, 83923 and 84254; Fax: 662-2002 extension 83922; Email: [email protected] ; http://sta.uwi.edu/asdlu/forms.asp 319 HOW TO STUDY FOR THIS COURSE Attendance is mandatory for lectures, tutorials and practical sessions. Prior preparation is strongly advised to enable you to fully participate in activities, and obtain the full value of the sessions. Thorough use should be made of the resources provided, and students are strongly advised to become familiar with them and start utilising them from the first week. Regular updates on course progress and materials are also highly recommended, and you should be checking into myeLearning on a frequent and regular basis to review materials, assignments and activities. Students are encouraged to interact regularly with staff on lecture materials and their assignments, even outside of the assigned lecture, tutorial and laboratory times, to ensure prompt, satisfactory solution of any problems and to monitor progress. Good group management is essential and requires good communication and interpersonal skills. GRADING SYSTEM The UWI grading scheme is as follows: http://sta.uwi.edu/resources/documents/Exam_and_GPA_regulations.pdf). Grade Quality point % range Grade definition A+ 4.30 90–100 Exceptional A 4.00 80–89 Outstanding A– 3.70 75–79 Excellent B+ 3.30 70–74 Very good B 3.00 65–69 Good B– 2.70 60–64 Satisfactory C+ 2.30 55–59 Fair C 2.00 50–54 Acceptable F1 1.70 45–49 Unsatisfactory F2 1.30 40–44 Weak F3 0.00 00–39 Poor 320 THE UNIVERSITY OF THE WEST INDIES, ST. AUGUSTINE FACULTY OF SCIENCE & TECHNOLOGY DEPARTMENT OF LIFE SCIENCES COURSE DOCUMENTATION 2015/16 COURSE CODE and TITLE NO. OF CREDITS LEVEL PREREQUISITES SEMESTER BIOL3971 Fisheries Management 3 3 BIOL3063 Marine Ecology and Oceanography 2 WELCOME LETTER TO STUDENTS Dear student, Welcome to BIOL3971 Fisheries Management. In this advanced-level course on the sustainable management of fishery resources, emphasis will be placed on the special issues related to the management of tropical commercial fisheries, especially those of the Caribbean region. This course is intended for those students who are interested in careers in the fields of fisheries biology and management. It offers a mix of theoretical knowledge and practical skills development, which are also valuable for those aiming for careers in marine biology, conservation, environmental consulting and management You will be introduced to several fisheries management models, and, as such, you are therefore expected to be able to do basic calculus and simple arithmetic, algebra and numerical analysis. Students must be comfortable working with relatively simple mathematical and statistical models. This course will be taught in blended mode comprising face-to-face lectures, tutorials and practical exercises, as well as online delivery of resource materials and diverse activities. You should engage with the course materials and participate fully to develop the essential skills of critical thinking (clarity, accuracy, relevance, logic, breadth, depth, precision, significance, completeness and fairness). Activities will be diverse, and you are strongly encouraged to ask questions, offer new ideas, problem-solve, and think innovatively to maximise the value of your learning experience. There will also be individual and group assignments, which should help you to develop communication and team skills that are important in the world of work. Please read the course outline carefully and keep the course schedule close to hand as it contains all the activities and assessments for the semester. In particular, take note of the policy on plagiarism and attendance requirements. We are here to ensure your success in this course and at UWI generally, and encourage you to come to us with any academic or other challenges you may face that could affect your attendance and performance. We look forward to meeting each of you and engaging in productive, stimulating discussions over the coming semester. Dawn A. T. Phillip Course coordinator INSTRUCTOR INFORMATION This course will be taught by a team of lecturers, teaching assistants and demonstrators. Further logistical support will be provided by administrative, laboratory and field support staff. Office hours are posted on departmental notice boards and the main Life Sciences office Course coordinator Lecturer Dr Dawn A.T. Phillip Lecturer Prof. Indar W. Ramnarine Teaching assistant Zaheer Hosein 2nd floor, new wing, Natural Sciences building 662-2002, ext. 82047 and 82208 [email protected] (preferred method of contact) Dean’s Office, 2nd floor, Chemistry 662-2002, ext. 84484 [email protected] (preferred method of contact) Teaching Assistant’s office, 2nd floor, new wing, Natural Sciences building, ext. 82204 [email protected] (preferred method of 321 contact) WEEKLY SCHEDULE Lectures and tutorials (You are required to attend all sessions) Practical classes (Weeks 3, 5, 7 and 9 – attend all sessions) Tuesdays Wednesdays Mondays 10:00–10:50 pm 10:00–10:50 am 2:00–06:00 pm C2 114 212A COURSE DESCRIPTION In BIOL3971 Fisheries Management, you will be introduced to concepts in the related fields of fisheries biology, stock assessment and fisheries management. The course covers major trends in global and regional fisheries and fishing patterns; human and environmental influences on productivity and sustainability; traditional fish stock assessment models; and traditional, modern and emerging strategies for managing fisheries. Emphasis will be placed on tropical fisheries, which are among the most difficult fisheries to manage. COURSE RATIONALE This course forms part of the specialisation in marine biology. Having completed BIOL3063 Marine Ecology and Oceanography, which is a pre-requisite for this course, you should now be familiar with basic oceanography, oceanic and atmospheric circulation, primary and secondary productivity, plankton cycles and distribution, all of which are key concepts in understanding the ecology of fisheries resources. Fisheries biology and management are critical disciplines in today’s world, given the importance of the fishing industry as a source of animal protein in the human diet; the basis of a multimillion-dollar industry; and the threatened status of many of the major species on which we depend. COURSE CONTENT Topics will include Diversity of fishery resources exploited by marine fisheries world wide and in the region The Law of the Sea and its implications for management of fishery resources Types of fishing gears and techniques used in commercial fisheries Fishers and fishery socioeconomics Issues and challenges facing commercial fish stocks, including direct and indirect effects of destructive fishing practices, and other anthropogenic factors such as climate change and habitat alteration Key topics in fisheries biology, such as fish reproduction; recruitment of juveniles to the fishery; diet and feeding; age and growth; fishing and natural mortality; stock structure; and other special topics Aims and objectives of fisheries management Fisheries management strategies and approaches, including traditional fishery management targets and indices, and more recent approaches such as participatory and ecosystem-based management Fisheries models as standard tools used by fishery managers, and the data requirements for fisheries management GOALS/AIMS This course aims to Using fishery resources as the focus, expand students’ knowledge of the range of aquatic animals exploited by humans for their use, and the consequences of over-exploitation of these biological resources Develop students’ competence in basic methods and approaches for fisheries stock assessment Develop students’ awareness that resource users are complex, social beings operating in various socio-cultural and socio-political contexts that must be understood by fisheries managers in order to effectively manage the exploitation of the resource base Introduce students to the range of options that have been used, and are being developed, to manage fishery resources GENERAL OBJECTIVES The general objectives of this course are to provide course materials, activities and assessments using both face-to-face and online modalities facilitate opportunities for students to engage with general concepts and specific examples to develop a broad understanding of the complex nature of human impacts on fishery resources guide students in crafting questions and critically analysing and exploring answers to these questions using investigative, hands-on approaches 322 facilitate discussion on the relationships between the impacts of humans on fishery resources, including impacts on the marine ecosystems that support these organisms, and evaluate potential solutions for ensuring sustainability of these exploited resources LEARNING OUTCOMES By the end of this course students should be able to: List a number of species that form the basis of fisheries at the global, regional and local scales, and discuss the trends and status of the fishery for specific named ones Outline the history and present status of global fisheries, and predict plausible future trends in these Discuss some of the factors that influence the productivity and sustainability of global, regional and local fisheries Describe the main factors that affect the dynamics of fish populations, and those that influence recruitment Discuss various fisheries management strategies and their limitations Describe some of the more important relationships between fish stocks, humans and the environment Explain why tropical fisheries are considered complex, and suggest options for their effective management Demonstrate basic skills for researching, analysing, synthesising, evaluating and communicating published and original fisheries information; evaluate specific human-environment interactions relevant to fisheries, and identify resulting sustainability issues; develop solutions using a variety of strategic management approaches; manage group interactions, and work effectively in teams. Students should also gain practical skills that will allow them to Describe a fishery based on either the target species, or fishing methods, or landing site/home port used by fishers Accurately identify commercially-important fishery species using species identification keys Sample fishery landings to collect fisheries data, particularly length, weight, sex and stage of maturity Dissect fin fish to collect stomach contents, otoliths and gonads, and process these for determination of diet, age, sex and gonad maturity Use fisheries data and models to determine reproductive cycles, mortality, growth, stock structure and various other fishery indices COURSE ASSESSMENT The final mark for this course comprises the following Theory examination: one 2-hour written examination (50%) In-course theory examination (10%) In-course practical exercises (10%) and group project (30%) ASSIGNMENTS In-course assignments comprise 4. A theory examination worth 10% is due as in the course schedule. It may include a variety of types of multiple-choice questions, true/false statements and short-paragraph questions. Any material covered in lectures, tutorials or practical sessions may be tested. 5. Practical reports, collectively worth 10% of the final course mark. The reports will be based on activities engaged in during four scheduled laboratory sessions as stipulated in the course schedule. 6. A single group project worth 30% of the final course mark [individual contribution to the project report (5%); individual participation during discussion sessions (5%); final project report (20%)]. The project will be a reading research project based on original literature and technical reports, and may incorporate results from the practical exercises EVALUATION Feedback on the course will be obtained informally from students on an on-going basis by regular interactions and meetings among students, demonstrators, teaching assistants and the course coordinator in lectures, tutorials and laboratory sessions. Formal feedback will be via election of class representatives who sit on the departmental Student-Staff Liaison Committee meetings held twice during the semester. Class representatives will channel both concerns and commendations to the meeting, as guided by the department’s standard operating procedures. Formal evaluation of the entire course will be accomplished via a UWI course evaluation questionnaire administered anonymously and confidentially at the end of the semester. All feedback will be considered on an on-going basis and corrective actions or adjustments made or discussed with students promptly, or incorporated the following year. TEACHING STRATEGIES 323 This course will be taught through a mixture of lectures, practical exercises and tutorials. There may also be guest lectures by experts from the government and private sector. In addition, the online teaching tool, myeLearning, will be used extensively during this course for communication among students and staff (email, discussions) official posting of important notices (coursework assessment notices, instructions, and in-course results) provision of course details, lecture notes, practical guides, tutorial briefings provision of recommended resource materials, and links to resources on specific web sites Face-to-face contact time will be 36 credit hours, comprising: Lectures 24 hours Lectures will provide valuable synthesis and evaluation of available information on fisheries biology, stock assessment and management, update current issues and management approaches, prioritise content relevant to course assessment. Case studies and selected topics may be presented by guest lecturers. Lecture sessions will be interactive, with students encouraged to ask questions, share knowledge and experiences. Course materials and readings will be available online in advance to allow students to be better prepared to engage the lecturers in discussion during class time Tutorials 4 hours Tutorials will cover course topics in an interactive format using a variety of collaborative active learning techniques. In addition to clarifying details of course content, specific transferrable skills may be addressed, e.g., essay and report writing, critiquing and oral presentation Practical exercises 16 hours Four 4-hr practical sessions will provide hands-on experience for students to gain skills required for conducting well-designed fisheries research. Some time will also be assigned for conducting discussions and brainstorming sessions for group projects, under the supervision of their demonstrators and lecturers. RESOURCES Most resources are available myeLearning, including Lecture outlines – including learning objectives, summaries, recommended readings, some presentations Resources – links to papers, articles and websites Tutorials – guidelines for tutorial activities, and specific supporting resources Practical exercises – guidelines for practical activities, and specific supporting resources (texts, articles, websites) Group project – detailed instructions and supporting websites to guide students in navigating the challenges of working on a group project. These will include information on working in teams, as well as some literature on the research topic. Essential and recommended texts are in the Reserve Section, Alma Jordan Library, or are available via links in the course outline and myeLearning. Essential readings Text books Berkes, F.; Mahon, R.; McConney, P.; Pollnac, R.; Pomeroy, R. 2001. Managing Small-Scale Fisheries: Alternative Directions and Methods. International Development Research Centre, Ottawa, Canada. [SH329.S53 M36 2001] Food and Agriculture Organisation (FAO) (ed.). 1997. The Law and the Sea: Essays in Memory of Jean Carroz. FAO, Rome. King, M. 2010. Fisheries Biology, Assessment and Management, 2nd edition. Blackwell Publishing, Oxford. Gabriel, O.; Lange, K.; Dahm, E.; Wendt, T.; von Brandt, A. (eds). 2005. Fish Catching Methods of the World, 4th edn. Blackwell Publishers, Ames, Iowa. [SH344.B69 2005] Technical reports and conference papers Ramnarine, I.W. 1998. Towards sustainable fisheries in Trinidad & Tobago. Agriculture in the Caribbean: Issues and Challenges, Vol. III, pp. 142–148. Continuing Education Programme in Agricultural Technology (CEPAT), 19–21 Aug 1998, The University of the West Indies, St. Augustine, Faculty of Agriculture and Natural Sciences. Caribbean Regional Fisheries Mechanism (CRFM). 2009. Report of 5th Annual Scientific Meeting – Kingstown, St. Vincent & the Grenadines, 9–18 June 2009 – National Reports. CRFM Fishery Report 2009. Volume 1, Supplement 1. CRFM Secretariat, Belize. CRFM 2011. Report of the 7th Annual Scientific Meeting, Kingstown, St Vincent and the Grenadines, 16–24th June 2011 – National Reports. CRFM Fishery Report 2011, Volume 1, Supplement 1, CRFM Secretariat, Belize. [For latest reports see CRFM website http://www.caricom-fisheries.com/Default.aspx] 324 Additional readings Books Ellis, R. 2004. The Empty Ocean. Island Press, Washington DC. [QL121.E5794 2003] Food and Agriculture Organisation (FAO). 2007. The State of World Fisheries and Aquaculture, 2006. FAO, Rome. Haddon, M. 2010. Modelling and Quantitative Methods in Fisheries, 2nd edn. CRC Press, Boca Raton. [SH331.5.M48 H34] Jennings, S., Kaiser, M., & Reynolds, J. 2001. Marine Fisheries Ecology. [SH328.J46 2001] Pitcher, T.; Hart, P.J.B. 1982. Fisheries Ecology. Kluwer Academic Publishers, Dordrecht, Netherlands. [SH328.P57 1983] Pitcher, T.J; Hart, P.; Pauly, D. (eds). 1998. Reinventing Fisheries Management. Fish and Fisheries Series, Vol. 23. Kluwer Academic Publishers, Dordrecht, Netherlands. [SH328.R45 1998] Journal articles Pauly, D. and Christensen (1995) Primary production required to sustain global fisheries. Letters to Nature, 374, 255–257. Pauly, D. Chirstensen, V.; Froese, R.; Palomares, M.L.D. (2000) Fishing down aquatic food webs. American Scientist, 88, 46–51. Pauly, D.; Christensen, V.; Guénette, S.; Pitcher, T.J.; Sumaila, U.R.; Walters, C.J.; Watson, R.; Zeller, D. (2002) Towards sustainability in world fisheries. Nature, 418, 689–695. Watson, R. and Pauly, D. (2001) Systematic distortions in world fisheries catch trends. Letters to Nature, 414, 534–536. Magazine and newspaper articles Law, R. (1991) Fishing in evolutionary waters: the way we exploit a fishery influences the way a population of fish evolves. Can we manage this evolution? New Scientist, 1758. Lemonick, M.D.; Aunapu, G.; Dorfman, A.; McDowell, J. (1997) Under attack. Time Magazine. Nash, J.M. (1997) The fish crisis. Time Magazine. ADDITIONAL INFORMATION Refer to the Life Sciences’ Undergraduate Handbook for general guidelines for teaching, assessment, student conduct, essential skills, and support at http://sta.uwi.edu/fst/lifesciences/documents/handbook.pdf. Refer to the general information and general regulations in the Faculty of Science and Technology’ Undergraduate Booklet available from http://sta.uwi.edu/resources/documents/facultybooklets/ScienceTechUndergrad.pdf. Attendance: Students are reminded that they must attend and report satisfactorily on a minimum of 75% of the practical sessions, lectures and tutorials. Failure to do so will result in debarment from the final examination (Extracts from Examination Regulations for First Degrees, Associate Degree, Diplomas and Certificates, section II (18) available from http://sta.uwi.edu/resources/documents/Exam_and_GPA_regulations.pdf). Medical and other excuses: Any student who misses a practical or lecture class, test or assignment is advised to consult immediately in person or by email with the teaching assistant or course coordinator regarding reasons for their absence and recoup options. Absence must be explained in writing within 7 days of the missed session by either a written/emailed excuse sent to the TA or course coordinator, and/or a medical certificate submitted to the Health Service Unit and copied to the secretary, main office, Department of Life Sciences. The department’s copy should have appended to it information on courses affected, course coordinator or TA responsible for those courses, and course events involved. Make-up sessions: As a general principle, medical or other reasons may only excuse a student’s presence at an assigned time. Students must still complete the assigned work (make-up lab report or make-up test) in order to obtain the marks for that item of course work. The student is responsible for liaising with the course coordinator or teaching assistant to ensure the assigned make-up exercise is completed. Any student who was inexcusably absent from a practical or test, or who does not submit a practical report (initial or make-up) will receive 0% for that exercise. Note that a field trip cannot be repeated for a make-up opportunity, so you should make every effort to attend field trips. Late submissions: A penalty at 10% of mark per day late will be applied unless formal approval for late submission is given by the teaching assistant or course coordinator using the guidelines above for medical or other excuses. Assignment submission: Hard copy reports must be submitted into the labelled deposit box for your bench/demonstrator in the outer Zoology office, and the student list must be signed. Submissions anywhere else will not be assessed. Reports will be returned at a time arranged by the TA or demonstrator. We are not responsible for reports left uncollected or submitted inexcusably late. Soft copy reports or other assignments must be submitted through myeLearning as directed for each assignment. Academic dishonesty: Academic dishonesty, which includes plagiarism and cheating, is an extremely serious academic offense, and carries penalties varying from failure in an assignment to possible suspension from the university. Students are hereby informed that plagiarism is forbidden, and all unsupervised coursework items must be accompanied by a completed plagiarism declaration form in order to be assessed. Specific items may require submission through Turnitin 325 on myeLearning. Refer to the University Regulations on Plagiarism available from http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf Safety in the laboratory and field: Medical data and emergency contact forms must be completed in the first laboratory session when safety briefings will be given. Standard laboratory protocols for conduct and dress code are in effect once you are present in a Life Sciences laboratory (Department of Life Sciences Undergraduate Student Handbook section 8). The dress code includes mandatory laboratory coats, adequate clothing to protect the body, and closed shoes. NO sandals, slippers or open-toed shoes will be allowed in the laboratory or in the field. Marks will be deducted for non-adherence to these protocols. Field work: Details are given in section 8 of the Department of Life Sciences Undergraduate Student Handbook http://sta.uwi.edu/fst/lifesciences/documents/handbook.pdf. Field trips are specified on the attached course calendar so you can be adequately prepared. You will not be allowed on the field trip if a medical data form has not been submitted. If you have a disability and have not disclosed the nature of your disability and the support you need, you are invited to contact the Academic Support/Disabilities Liaison Unit (ASDLU) of The University of the West Indies, St. Augustine Campus. Phone: 662-2002 extension 83866, 83921, 83923 and 84254; Fax: 662-2002 extension 83922; Email: [email protected] ; http://sta.uwi.edu/asdlu/forms.asp. HOW TO STUDY FOR THIS COURSE Firstly, this is not a course where you can rote-learn factual information at the last minute for a test, or prepare simplistic essays copied off the Internet for assignments. It requires familiarity with a wide range of topics, informed discussion of broad, complex issues and application of critical thinking skills. You should attend all lectures, tutorials and practical sessions since satisfactory attendance (>75%) is required by the UWI regulations. Attendance records will be taken at all sessions. Students who attend regularly perform better than those who do not. You should attend to obtain the full value of the lecturer’s presence. Lecturers routinely synthesise and summarise large amounts of information, and prioritise, evaluate, discuss and explain this information. They provide the conceptual framework of the course, which you must then fill in with details and examples through independent study. You should prepare before classes so you can clarify any difficult points with the lecturer, or engage in meaningful discussion of any controversial topics. This will enhance and deepen your understanding as well as contribute to course assessment in tutorials and practical sessions. This is your opportunity for continuous assessment to monitor your progress and to accumulate coursework marks incrementally. You should review learning outcomes for each topic or activity, and make sure you understand and can actually achieve those outcomes. If you cannot, please clarify with the lecturer. You must make thorough use of the resources provided (e.g., textbooks, resouces on myeLearning) from the first week. You should check into myeLearning frequently to review updated materials, assignments, notices and activities. In addition to timetabled contact time, you are expected to devote additional hours of independent study: reviewing class materials and notes, reading/viewing/using resource materials, completing assignments, and preparing for assessments. You are strongly encouraged to make your own notes from lectures, textbooks and other source materials throughout the semester to improve your skills of comprehension, organisation, planning and writing. Working in study groups is strongly supported for those who learn best this way, as it shares workloads, builds camaraderie and social skills; however, please do not rote-learn prepared essays, and always double check the accuracy of your colleagues’ work. You are encouraged to interact regularly with staff, even outside of the assigned class times to ensure prompt, satisfactory resolution of any problems and to monitor progress. Attendance is mandatory for lectures, tutorials and practical sessions. Prior preparation using online course materials is strongly advised to be able to fully participate in activities, and obtain the full value of the sessions. Thorough use should be made of the resources provided, and students are strongly advised to become familiar with them and start utilising them from the first week. Regular updates on course progress and materials are also highly recommended and you should be checking into myeLearning on a frequent, regular basis to review materials, assignments and activities. Some online activities will contribute to your final grade (see course assessment). Students are encouraged to interact regularly with staff on their projects, even outside of the assigned laboratory times, to ensure prompt, satisfactory resolution of any problems, and to monitor progress. 326 Grading scheme: Grading Scheme used in the Faculty of Science & Technology is as follows: Mark Grade Grade Descriptor Quality Points 90-100 A+ Exceptional 4.3 80-89 A Outstanding 4.0 75-79 A- Excellent 3.7 70-74 B+ Very Good 3.3 65-69 B Good 3.0 60-64 B- Satisfactory 2.7 55-59 C+ Fair 2.3 50-54 C Acceptable 2.0 45-49 F1 Unsatisfactory 1.7 40-44 F2 Weak 1.3 0-39 F3 Poor 0 327 COURSE CALENDAR W k 1 Dat e T19 Lecture topics 1. Introduction to this course (DATP) 2. Diversity of fishery resources (DATP) 3. Fishing gear and commercial fishing methods (IR) 4. World fisheries, history, status and future (IR) 5. Law of the Sea – implications for fisheries (DATP) 6. Destructive fishing methods – e.g., incidental catches and ghost fishing (ZH) Consequences for stocks – e.g., overfishing, fishing down the food chain (no lecture) Other anthropogenic effects – e.g., climate change, habitat destruction (DATP) Practical exercises Assignments . 1 W2 0 . 1 2 T26 . 1 W2 7 . 1 3 T2. 2 4 W3 .2 T9. 7. 2 W1 8. 0 . 2 5 6 T16 .2 W1 7.2 T23 9. 10. Fisheries biology – food and feeding energetics (DATP) W2 4 . 2 T1. 3 8 Practical report due (2.5%) M15.2 Diet and feeding 11. Fisheries biology – reproduction and recruitment (DATP) . 2 7 Effects of fishing on the ecosystem (DATP) M1.2 Fish identification, bycatch statistics W2 .3 T8. In-course theory quiz (10%) 12. Fisheries biology – age and growth (RK) 13. Fisheries biology – mortality, abundance and stock structure (DATP) 14. Fisheries biology – critical habitats, e.g., spawning and nursery grounds (GM) 15. Aims of fisheries management (IR) M14.3 Reproduction and mortality Practical report due (2.5%) 3 W9 16. Data requirements for fisheries management (IR) . 3 9 T15 . 3 W1 17. Fishery models – surplus-yield models (IR) M28.3 Age and growth 18. Fishery models – dynamic-pool models (IR) 6 . 3 1 0 T22 19. Fisheries management strategies and approaches (IR) . 3 W2 3 20. Special issues related to tropical fisheries management (IR) Practical report due (2.5%) . 3 1 1 T29 21. Fishers and fisheries socioeconomics (DATP) W3 22. Community-based fisheries management case study (DATP) 0 . 3 1 2 Group project due (30%) . 3 T5. 23. Caribbean fisheries management project (DATP) 4 W6 Practical report due (2.5%) 24. Fisheries management – future directions4 (DATP) . 4 1 3 M4 DATP GM IR RK ZH Tutorials, including exam preparations . 4 DAWN A T PHILLIP GUY MARLEY INDAR RAMNARINE (PROF) ROSEMARIE KISHORE ZAHEER HOSEIN The University of the West Indies, St. Augustine Faculty of Science & Technology Department of Life Sciences COURSE CODE & TITLE: BIOL XXXX CARIBBEAN ISLAND ECOLOGY AND BIOGEOGRAPHY NO. OF CREDITS: 3 LEVEL: 3 SEMESTER: 1 PREREQUISITES: EITHER (BIOL 2464 Fundamentals of Ecology AND BIOL 2163 Biostatistics) OR BIOL1462 General Ecology and Biometry 4 For example FISHE (http://fishe.edf.org/); new and upcoming alternative fishing techniques, such as electrotrawling (Soetaert et al. 2013 and errata) 329 Lectures (27 credit hours); Labs/Field trips (10 Credit hours- 0.5 credit hours per contact hour). Total Credit Hours: 37 This course has been reviewed by the Board of Faculty of Science and Technology. COURSE DESCRIPTION: This advanced course treats the islands of the Caribbean within a global perspective. Its subject is the special nature of island environments and their biotas, and its aim is an understanding of the distributions and ecological relationships of island plants and animals through an analysis of their origins, evolutionary past, population ecology and community composition. The course is expected to integrate much of the knowledge that advanced undergraduates have amassed. Teaching for the course will be approached in a blended/hybrid replacement classroom manner with asynchronous lectures presented online with recorded video and audio and select, classroom sessions. Practical exercises involving field work, literature review and synthesis work and exercises will also be applied to gain more practical skills. Assessment will be based on coursework (50%) and a final theory exam (50%). Course work will be in the form of written reports of practical exercises and literature assignments. COURSE RATIONALE: This is an elective course for the BSc (Biology Major) students and in the future, for the Ecology and Environmental Biology Specialization in the BSc (Biology with Specialisations) in Life Sciences that will provide fundamental knowledge and concepts of Caribbean biodiversity that students can apply in most areas they may encounter in Environmental Biology. At the end of the course the students will be able to discuss the complex origins, characteristics and values of Caribbean biodiversity; the nature of threats due to human activities including global climate change; and evaluate realistic responses to conserve Caribbean island biodiversity and how it responds to human manipulation. INSTRUCTOR INFORMATION: This course will be taught by a team of lecturers, Teaching Assistants and demonstrators. Office hours are posted on Departmental notice boards and the main Life Sciences office. Name of instructor(s): Mike Oatham (Coordinator); Linton Arneaud (Teaching Assistant) Office address and phone: Rm 222, Old Wing, Natural Sciences Building (ext 83088) Email address: [email protected] Office hours: Monday 9am-11am; Tues 10am-12pm Preferred method of contact: Email 330 Communication policy: Students should use their UWI email account for communication and can expect a response within 48 hours. LETTER TO THE STUDENT: Welcome to ‘Caribbean Island Ecology and Biogeography’. In this course you will be challenged to develop the fundamental knowledge, skills and attitudes of a practising Caribbean ecologist. This is a course with theory and practical components that will require commitment and participation on your part to successfully engage in the content and to develop the essentials skills of critical thinking (clarity, accuracy, relevance, logic, breadth, depth, precision, significance, completeness and fairness). You are strongly encouraged to ask questions, offer new ideas, problem solve and think innovatively to maximise the value of your learning experience. In addition, teamwork, organisation and time management will be necessary personal attributes to bring to the course. CONTENT Topics will include: 1. General features of islands. History of island formation, the distinction between continental and oceanic islands. 2. Climate and seasonality in the Caribbean including extreme events such as hurricanes. 3. Development and composition of island biotic communities. Modes of colonisation, speciation and adaptive radiation, extinction. Species-area relationships. 4. Case histories of selected islands and archipelagos. 5. Island-like situations on continents. Consequences of ecosystem fragmentation. 6. Human impacts on island ecosystems plus management and adaptation to the effects of global climate change. GOALS/AIMS The goals of this course are to Describe the characteristics of Caribbean biodiversity and distribution of biodiversity in the Caribbean Examine the reasons why biodiversity occurs with these characteristics at these levels in different parts of the Caribbean Expose students to the currently accepted approaches to investigating island ecology 331 Provide opportunities to develop transferrable skills within a specific context Provide fundamental preparation and training in general practical and research skills Introduce specialist practical and research skills and approaches Inculcate a culture of quality and responsibility in conducting research E ha e stude ts apa it to ork effe ti el i real life situatio s LEARNING OUTCOMES At the end of this course students should be able to 1. Describe the different levels and characteristics of biodiversity in the Caribbean. 2. Describe the mechanisms for biodiversity patterns in the Caribbean and discuss the reason for them with specific references to Trinidad and Tobago. 3. Describe the biogeographical processes at work in the Caribbean. 4. Explain how characteristics of species and taxa and their dispersal mechanisms influence their patterns of distribution in the Caribbean. 5. Describe the physical characteristics of Caribbean islands and discuss how they influence the species and taxa distribution. 6. Describe the human impacts on island ecosystems and discuss how these are likely to influence Caribbean island biodiversity into the future. 7. Effectively find, evaluate and manage published information from a variety of sources including books, journal articles and web resources. 8. Use spreadsheet, word processing and presentation software to carry out tasks related to critically assessing and synthesizing reports from the scientific literature. 9. Review basic ethical principles for appropriate scientific conduct, for example citing references and writing to avoid plagiarism 10. Function effectively in real life situations by integrating knowledge, skills and attitudes with innovation and problem-solving approaches 11. Demonstrate effective oral and written communication 12. Demonstrate effective observation and record-keeping skills 13. Demonstrate effective skills in managing time, people and projects 14. Demonstrate skills in evaluating, managing and communicating information in ecology 332 COURSE ASSESSMENT Assessment will be based on a student’s final mark from the coursework components below. Component % Final Grade Learning Outcomes Addressed 100% broken down as follows: 20%. Paper 3, 4, 5, 6, 7, 8, 9, 11, 13, 14 10% Poster 3, 4, 5, 6, 7, 8, 9, 11, 13, 14 5%. Affinity Patterns Among Caribbean Islands 1, 2, 3, 4, 7, 10,11, 14 5% Long Distance Dispersal of Propagules 1, 2, 3, 4, 7, 8, 9, 10, 11, 12, 13, 14 5% Video Lab for Island Biogeography 1, 2, 3, 4, 7, 8, 9, 10, 11, 12, 13, 14 5% Cloud Forests or Sky Islands 1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 13, 14 50% 1, 2, 3, 4, 5, 6, 11, 14 Theory coursework Practical coursework Final Theory Exam ASSIGNMENTS Theory coursework Theory coursework (30%) will be a paper on an individualized topic (e.g. a particular island or a taxon with special relevance to islands) and a poster presentation. Topics will be assigned early in the course, with further guidance from lectures. Each student will submit a written paper by week 6 and do the poster presentation of his/her topic during week 8. The written paper will be worth 20% of the theory coursework and the poster presentation 10%. The contrasting methods of communicating the information will allow the students to understand the value in different contexts. Practical coursework 333 4 Practical Reports (5% each for a total of 20%). Writing up reports of practical exercises is of paramount importance. You must hand in your report or question sheet in the time specified or you will lose 10% of your mark for the assignment per day the report is late (this includes weekends). If the student is absent from a practical due to a medical condition they must still submit a report on the same date as all other students unless specifically excused by the course coordinator. It is the responsibility of the student to obtain any data set required to write up the report. EVALUATION: Feedback on the course will be obtained informally from students on an ongoing basis by regular interactions and meetings among students, demonstrators, instructors, teaching assistants and the Course Coordinator in practicals and tutorials. Formal feedback will be via election of Class Representatives who sit on the Departmental Student-Staff Liaison Committee meetings held twice during the semester. Class reps will channel both concerns and commendations to the meeting as guided by the Department’s Standard Operating Procedures. Formal evaluation of the entire course will be accomplished via a UWI Course Evaluation questionnaire administered anonymously and confidentially at the end of the semester. All feedback will be considered on an ongoing basis and corrective action or adjustments made or discussed with students promptly or incorporated the following year. TEACHING STRATEGIES The course will be taught in a blended/hybrid replacement classroom manner as a series of face to face tutorials reinforcing the concepts introduced in recorded lectures posted on myelearning. Prompt questions will be provided for each online lecture to give the student and lecturer an idea of how the student is progressing through the course. The face to face tutorials will take the form of: group completion of a model exam question on the topic; a MCQ or fill in the blank session on the topic; role playing assignment on the topic or an informal question and answer session. Coursework theory assignments are designed to make the students search the literature for relevant information and report it in a structured way. READINGS The following Readings will be required in the first instance and more recent and other relevant readings will be added and will replace other readings in future years. 334 Lomolino, M.V., Riddle,B.R., and Brown, J.H. 2005. Biogeography. 3rd Ed. Sinauer Associates, Sunderland Carlquist, S. 1974. Island Biology. New York: Columbia Univ. Press 660 pp. Cooper, St.G.C. & P.R. Bacon 1981. The Natural Resources of Trinidad and Tobago. London: Edward Arnold 222 pp. Gillespie, R.G. & D.A. Clague (eds.) 2009. Encyclopedia of Islands. Berkeley: Univ. California Press 1074 pp. Whittaker, R.J. 1998. Island Biogeography. Oxford: Oxford Univ. Press 285 pp. Williamson, M. 1981. Island Populations. Oxford: Oxford Univ. Press 286 pp. COURSE CALENDAR Week Topic Lectures 1 Islands. What and Where are They? Introduction Overview of main islands and archipelagos 2 The Physical Setting of Caribbean Islands Processes in island formation; Geological history of the Caribbean Caribbean climate and seasons Tutorial: Assignment of coursework topics 3 Island Environments and Ecosystems 4 Getting to Remote Islands and Biogeographic Processes on Continental Islands 5 The Fate of Island Species Island environments: Land, freshwater, coastal Remote or Oceanic Islands. Physical realities and biotic distinctiveness Over-water dispersal: Challenges and vagaries Vicariance and relaxation Tutorial: Species Area Relationship and the MacArthurWilson Dynamic Equilibrium Theory The fate of island species. Speciation The fate of island species. Extinction Tutorial: Adaptive Radiation Practicals Affinity patterns among Caribbean islands (Lab Exercise). Due after the lab Long-distance dispersal of propagules (field trip. Manzanilla Beach) Due one week after the Field Trip 335 Week Topic Lectures 6 Selected Islands Outside of the Caribbean Video Lab for Island Selected islands outside the Biogeography (Question sheet Caribbean: Madagascar, completed in the Lab) Due after Galápagos Islands the lab Selected islands outside the Caribbean: New Guinea, Hawaiian Islands Social insects Habitat "islands" in continental settings 7 Island Taxa Groups 8 Poster Presentations Poster Presentations 9 Island Taxa Groups Sedentary coastal faunas Land snails 10 Island Taxa Groups Land vertebrates: Amphibians and reptiles Land vertebrates: Birds and mammals Freshwater faunas Vascular plants 11 Island Taxa Groups 12 Humans on Islands 13 Course Review Practicals Cloud Forests or Sky Islands (Half-day field trip, Arima Valley). Due one week after the Field Trip. Human impacts Future of island ecosystems HOW TO STUDY FOR THIS COURSE Attendance is mandatory for lectures, tutorials and practicals. Full use should be made of the resources provided and students are strongly advised to become familiar with them and start utilising them from the first week. 336 Students are encouraged to interact regularly with their lecturer on their projects, even outside of the assigned tutorial times to ensure satisfactory solution of any problems and to monitor progress. ADDITIONAL INFORMATION: Refer to the Life Sciences Undergraduate Handbook for general guidelines for teaching, assessment, student conduct, essential skills, and support at http://sta.uwi.edu/fst/lifesciences/documents/handbook.pdf . Refer to the General Information and General Regulations in the Faculty Booklet available from http://sta.uwi.edu/resources/documents/facultybooklets/ScienceTechUndergrad.pdf . Attendance: Students are reminded that they must attend and report satisfactorily on a minimum of 75% of the practical sessions and tutorials. Failure to do so will result in debarment from the final examination. Medicals, excuses: Any student who misses a class, test or assignment is advised to consult immediately in person or by email with the TA or Course Coordinator regarding reasons for their absence and make-up options. Absence must be explained in writing within 7 days of the missed session by either a written/emailed excuse sent to the TA or Course Coordinator, and/or a medical certificate submitted to the Health Service Unit and copied to the Departmental Secretary, Life Sciences and the Course Coordinator. Makeups: As a ge eral pri iple, edi als or other e uses a o l e use a stude t s presence at an assigned time. Students must still complete the assigned work (make-up lab report or make-up test) in order to obtain the marks for that item of coursework. The student is responsible for liaising with the Course Coordinator or Teaching Assistant to ensure the assigned make-up is completed. Normally only ONE make-up session will be held. Any student who was inexcusably absent from a practical or test or does not submit a practical report (initial or make-up) will receive 0% for that exercise. Note that a field trip cannot be repeated for a make-up so you should make every effort to attend field trips. Late submission: A penalty at 10% of mark per day late will be applied unless formal approval for late submission is given by the TA or Course Coordinator using the guidelines above for medicals, excuses. Assignment submission: You will be informed whether reports should be in HARD or SOFT copy format. HARD COPY reports should be submitted in the practical session or afterwards into a labelled deposit box for your bench/demonstrator in the outer Zoology office and the student list must be signed. Submissions anywhere else will not be assessed. These will be returned at a time arranged by the TA or demonstrator. We are not responsible for reports 337 left uncollected or submitted inexcusably late. SOFT COPY reports should be submitted as instructed through the relevant assignment link on the BIOLXXXX myelearning page. Plagiarism: Students are hereby informed that plagiarism is forbidden and all unsupervised coursework items must be accompanied by a completed Plagiarism Declaration form in order to be assessed. Specific items may require submission through Turnitin on eLear i g. ‘efer to U i ersit ‘egulatio s o Plagiaris a aila le fro http://sta.uwi.edu/resources/documents/Exam_Regulations_Plagiarism.pdf Safety in the lab and field: Medical Data and Emergency Contact forms must be completed in the first lab session when safety briefings will be given. Standard laboratory protocols for conduct and dress code are in effect once you are present in a Life Sciences laboratory (Undergraduate Student Handbook section 8). Dress code includes mandatory lab coats, adequate clothing to protect the body, and closed shoes. NO sandals, slippers or open-toed shoes will be allowed in the lab or field. Marks will be deducted for non-adherence to these protocols. Field work: Details are given in section 8 of the Undergraduate Student Handbook http://sta.uwi.edu/fst/lifesciences/documents/handbook.pdf . Field trips are specified on the attached Course Calendar so you can be adequately prepared. You will not be allowed on the field trip if a Medical Data form has not been submitted. Accessibility: If you have a disability and have not disclosed the nature of your disability and the support you need, you are invited to contact the Academic Support/Disabilities Liaison Unit (ASDLU) of The University of the West Indies (UWI), St. Augustine Campus. Phone: 6622002 extension 83866, 83921, 83923 and 84254; Fax: 662-2002 extension 83922; Email: [email protected] ; http://sta.uwi.edu/asdlu/forms.asp 338 GRADING SYSTEM: The NEW UWI grading scheme effective 2014/15 is given below. See http://www.uwi.edu/gradingpolicy/ for details. GRADE QUALITY POINT % RANGE Grade Definition A+ 4.30 90-100 Exceptional A 4.00 80-89 Outstanding A- 3.70 75-79 Excellent B+ 3.30 70-74 Very good B 3.00 65-69 Good B- 2.70 60-64 Satisfactory C+ 2.30 55-59 Fair C 2.00 50-54 Acceptable F1 1.70 45-49 Unsatisfactory F2 1.30 40-44 Weak F3 0 0-39 Poor 339 THE UNIVERSITY OF THE WEST INDIES ST. AUGUSTINE, TRINIDAD AND TOBAGO, WEST INDIES THE CENTRE FOR EXCELLENCE IN TEACHING AND LEARNING OFFICE OF THE CAMPUS PRINCIPAL Telephone: (868) 662-2002 Ext. 82214, 82611 or 224-3736 Fax: (868) 662-0558 e-mail: [email protected] MEMORANDUM TO: Dr. G. Briggs Life Sciences-Biology FROM: Dr Margo Burns Director, CETL DATE: 16/04/2016 SUBJECT: Revision to Minor in Biology This letter confirms that the courses associated with the proposed revision to the Minor in Biology were approved by the Centre for Excellence in Teaching and Learning during the 1052016 Academic Year. And as such, the proposed revision to the Minor in Biology has been reviewed and approved. As agreed upon, all courses associated with the Minor in Biology will have the revised GPA structured inserted into the course outlines for the Academic Year 2016-2017. Congratulations on completing the revision to the Minor in Biology. Kind regards, Margo P. Burns, EdD., MPH, MSc(A), RRT(A) Cc: Secretary, Secretariat, Office of the Campus Registrar MPB/ Comments from Mona (24 February 2016): - the proposal was circulated among staff. - overall, there were no major queries/concerns. It is a coherent programme of study and training in the biological sciences. - the programme provides exposure to the fundamental principles of biology, including studies of the structure, organization, and diversity of life. - it was noted that the layout of the credits on page 2 could be more clearly explained, as initially it seems that the total advanced credits for the minor is 24. Comments from Cave Hill (25 February 2016) - the proposal is straightforward and well-justified in your document. You have our full support.
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