ELISA Kit for Environmental Pollutants
Microcystins/Nodularins
Microcystins-DM ELISA Kit
◊ Patented technology. The monoclonal antibody binds with microcystins and
nodularins, allowing the determination of these toxins and many of its
congeners, and does not cross-react with other non-related toxins or
compounds.
◊ The assay range is between 0.15 ppb and 5 ppb. A sensitive assay to determine
microcystins/nodularins concentration in environmental samples.
◊ Direct assay, no pre-incubation steps necessary. Total time for measurement is
less than 2 hours.
◊ The kit, a 96-well microplate format with ready to use color coded reagents,
enables simultaneous measurement of multiple samples at a reasonable cost.
Chemical Structure
Microcystins/Nodularins are toxins produced by
cyanobacteria (blue-green algae).
Acute poison in
humans and animals can be caused by these toxins,
and in several cases has led to death.
These toxins
inhibit liver function and might act as tumor promoters.
To protect consumers from adverse health effects
caused by these types of toxins, the WHO has proposed
a provisional upper limit for mycrocystins-LR of 1000
ppt in drinking water.
Many different structural
variants or congeners of mycrocystins and nodularins
are found, the most common variant is mycrocystin-LR.
HPLC or the PPA, are generally employed methods for
quantitative microcystin analysis, however, they require
expensive instrumentation as well as complex
procedures. This ELISA test kit allows for the detection
of congener-independent microcystins/nodularins toxins
in environment samples at the ppt levels.
Microcystins Standard Curve
1.0
Samples containing mycrocystins/nodularins
within the dynamic range (0.15 – 5 ppb) can be
directly tested in the assay after filtration.
B/Bo (%)
0.8
0.6
0.4
0.2
0.0
0.15
0.4
1
Concentration (ng/m L)
2
5
Cross-reactivity Pattern
Cross-reactivity against microcystins and nodularin congeners.
Specificity
The cross-reactivity of the Abraxis Microcystins-DM ELISA for various
Microcystins congeners can be expressed as the least detectable dose (LDD)
which is estimated at 90% B/Bo, or as the dose required for 50% absorbance
inhibition (50% B/Bo).
LDD
50% B/Bo
X-reactivity
Compound
(ppb)
(ppb)
(%)___
Microcystins LR
0.093
0.066
100
Microcystins YR
0.120
1.03
64
Microcystins RR
0.193
1.24
53
Microcystins LA
0.210
1.39
48
Nodularins
0.05
0.87
76
N-hemi-ADDA
0.105
1.80
38
ADDA
0.62
4.85
15
D-Phenylalanine
NR
NR
NR
L-Phenylalanine
NR
NR
NR
DL-Phenylalanine
NR
NR
NR___
NR = no reactivity up to 1000 ppb
References
•
•
•
U.S. Patent 6,967,240
Worldwide Patent PCT WO 01/18059 A2
M. G. Weller, A. Zeck, A. Eikenberg, S. Nagata, Y. Ueno, and R. Niessner,
Development of a Direct Competitive Microcystins Immunoassay of Broad
Specificity. Analytical Sciences. 17, 2001, 1445-1448
Kit Format
Microplate (96T) and reagents PN 522015
Manufactured by
Abraxis LLC
54 Steamwhistle Drive
Warminster, PA 18974
Phone: (215) 357-3911
FAX:
(215) 357-5232
Email: [email protected]