3M™ Molecular Detection System
Listeria monocytogenes Protocol Reference Guide
Enrichment
(See back page for Enrichment Protocols)
Lysis
1. Transfer 20µL of Sample into individual Lysis (LS) tube.
LS
20µL NC
2. T ransfer 20µL of Negative Control (NC) into one LS tube.
3X
3. Cover rack of LS tubes with lid and invert 3 times to mix.
4. P lace rack with LS tubes into a heating block (100°C ± 1°C)
and heat for 15 minutes.
1. T ransfer 20µL of Sample
into individual LS tube.
2. Transfer 20µL of NC
into one LS tube.
3. Cover rack with lid.
Invert 3 times to mix.
5. Remove the rack of LS tubes from heating block and remove
lid. Place rack on Chill Block for 10 minutes.
6. R
emove the rack from Chill Block. Cover the rack and mix
3 times. Tap rack on bench 3 times.
99-101ºC
0-20ºC
7. Let set on bench top at room temperature for 5 minutes.
4. Heat LS tube rack at
100°C (±1°) for 15 minutes.
5. C
ool LS tube rack without
lid in Chill Block for 10 minutes.
Amplification
8. Transfer 20µL of each sample lysate into Reagent tubes. Mix by
gently pipetting up and down 5 times. Seal tubes with caps.
9. If needed, transfer 20µL of sample lysate into MC tubes. Mix by
gently pipetting up and down 5 times. Seal tubes with caps.
10. T ransfer 20µL NC lysate into one Reagent tube. Mix by gently
pipetting up and down 5 times. Seal tube with cap.
11. Transfer 20µL NC lysate into one Reagent Control (RC) tube. Mix
by gently pipetting up and down 5 times. Seal tube with cap.
3X
3X
6. C
over rack. Invert 3 times to mix.
Firmly tap rack on lab bench 3 times.
20-25ºC
7. L et set on bench top at room
temperature for 5 minutes.
20µL
12. Transfer closed tubes to Speed Loader Tray.
13. P lace Speed Loader Tray into instrument and close the lid to
start the assay.
8–11. Reagent hydration. Pipette
up and down 5 times to mix.
13. P lace Speed Loader Tray into
Instrument and close the lid.
12. T ransfer closed tubes
to Speed Loader Tray.
Results in 75 minutes.
3M™ Molecular Detection System
Listeria monocytogenes Protocol Reference Guide (cont.)
Enrichment
Environment
1 Sponge
225mL 3M™ Demi Fraser Broth Base
26–30 hr at 37°C (± 1°)
Processed Dairy
1 Swab
100mL 3M™ Demi Fraser Broth Base
26–30 hr at 37°C (± 1°)
10mL 3M™ Demi Fraser Broth Base
26–30 hr at 37°C (± 1°)
Other Foods*
25g
25g
225mL 3M™ Demi Fraser Broth Base
24–26 hr at 37°C (± 1°)
225mL 3M Demi Fraser Broth Base
24–28 hr at 37°C (± 1°)
™
Transfer 0.1mL into 10mL 3M™ Fraser Broth Base
24–26 hr at 37°C (± 1°)
*Other foods include meat, poultry, fish, seafood, produce, vegetables, vegetable based products and raw dairy.
NOTE: Do not use Demi Fraser Broth Base or Fraser Broth Base containing Ferric Ammonium Citrate (FAC), either already
in the broth base or as an added supplement, when using the 3M Moleculor Detection Assay Listeria Monocytogenes.
Food Safety
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www.3M.com/3MMolecularDetectionSystem
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