Amino acids, cofactors and others
reagent
molar mass
L-Aspartic acid
CoA Na-salt
E. coli tRNA
Folinic acid (Ca-salt hydrate)
Glycine
L-Alanine
L-Arginine
L-Asparagin monohydrat
L-Cysteine
L-Glutamine
L-Glutamic acid
L-Histidine
L-Isoleucine
L-Leucine
L-Methionine
L-Threonine
L-Tryptophan
NAD
Na-Oxalate
L-Phenylalanine
Phosphoenolpyruvate (PEP)
L-Proline
Putrescine
Spermidine
L-Valine
K-Glutamate
Mg-Glutamate
133.10
767.54
–
473.44
75.07
89.09
174.20
150.14
121.16
146.14
147.13
155.15
131.18
131.18
149.21
119.12
204.23
663.43
134.00
165.19
168.04
115.13
88.15
145.25
117.15
203.23
388.61
final concentration in
the reaction in mM
mass in g for 1500
reactions à 15 µl
2.00
0.27
–
0.07
2.00
2.00
2.00
2.00
2.00
2.00
2.00
2.00
2.00
2.00
2.00
2.00
2.00
0.33
4.00
2.00
33.00
2.00
1.00
1.50
2.00
80.00
4.00
5.99E-03
4.66E-03
3.83E-03
7.67E-04
3.38E-03
4.01E-03
7.84E-03
6.76E-03
5.45E-03
6.58E-03
6.62E-03
6.98E-03
5.90E-03
5.90E-03
6.71E-03
5.36E-03
9.19E-03
4.93E-03
1.21E-02
7.43E-03
1.25E-01
5.18E-03
1.98E-03
4.90E-03
5.27E-03
3.66E-01
3.50E-02
dose size in g
100
0.01
0.1
0.25
500
1
100
25
2.5
100
100
5
1
25
5
1
1
0.25
100
100
0.25
0.01
0.1
1
1
100
250
cost in $ for dose size
(September 2015)
18.51
57.58
178.45
70.58
44.50
10.86
46.81
21.00
12.28
56.60
21.27
11.66
12.73
17.71
11.66
10.86
12.10
52.24
24.30
75.21
92.12
18.60
28.57
27.59
14.06
50.91
43.70
amount for 1500
reactions per
dose
5.99E-05
4.66E-01
3.83E-02
3.07E-03
6.76E-06
4.01E-03
7.84E-05
2.70E-04
2.18E-03
6.58E-05
6.62E-05
1.40E-03
5.90E-03
2.36E-04
1.34E-03
5.36E-03
9.19E-03
1.97E-02
1.21E-04
7.43E-05
4.99E-01
5.18E-01
1.98E-02
4.90E-03
5.27E-03
3.66E-03
1.40E-04
cost for 1500
reactions
1.11E-03
2.68E+01
6.83E+00
2.17E-01
3.01E-04
4.35E-02
3.67E-03
5.68E-03
2.68E-02
3.72E-03
1.41E-03
1.63E-02
7.51E-02
4.18E-03
1.57E-02
5.82E-02
1.11E-01
1.03E+00
2.93E-03
5.59E-03
4.60E+01
9.64E+00
5.67E-01
1.35E-01
7.41E-02
1.86E-01
6.11E-03
9.19E+01
cost for one reaction
7.39E-07
1.79E-02
4.55E-03
1.44E-04
2.00E-07
2.90E-05
2.45E-06
3.78E-06
1.79E-05
2.48E-06
9.39E-07
1.09E-05
5.01E-05
2.79E-06
1.04E-05
3.88E-05
7.42E-05
6.86E-04
1.95E-06
3.73E-06
3.06E-02
6.42E-03
3.78E-04
9.02E-05
4.94E-05
1.24E-04
4.08E-06
6.12E-02
NTPs
reagent
molar mass
ATP 100 mM
GTP 100 mM
UTP 100 mM
CTP 100 mM
573.10
523.18
550.10
549.10
final concentration in
the reaction in mM
Volume in mL for 1500
reactions à 15 µl
1.5
1.5
0.9
0.9
0.3375
0.3375
0.2025
0.2025
dose size in mL
0.25
0.25
0.25
0.25
cost in $ for dose size
(September 2015)
27.50
27.50
27.50
27.50
amount for 1500
reactions per
dose
1.35
1.35
0.81
0.81
cost for 1500
reactions
37.13
37.13
22.28
22.28
1.19E+02
cost for one reaction
2.48E-02
2.48E-02
1.49E-02
1.49E-02
7.92E-02
Extract for 1500 reactions à 15 µL
medium
reagent
cost in $ for 5 L of
2xYT+P medium
K2HPO4
3.94
KH2PO4
1.00
0.76
10.08
7.94
23.72
NaCl
Tryptone
Yeast extract
DTT
S30A washing buffer
cost for 1 L of
reagent
S30A washing
buffer
0.10
K-Glutamate
Mg-Glutamate
6.21
0.95
7.26
30.98
All together
Amino acids,
cofactors and
others
NTPs
Extract
TOTAL
Costs for 1500 cost in $ for a single 15
reactions
µL CFPS reaction
91.87
0.061
118.80
0.079
30.98
241.66
0.021
0.161
subtotals with green background