allelebiotech.com
Allele Biotechnology & Pharmaceuticals, Inc.
Alleleustrious pmWasabi-C
Recommended Use
(C mammalian expression)
mWasabi has been optimized for use with standard GFP/FITC filter
sets.
Features
Catalog Number:
ABP-FP-WCNCS10
Size: 10ug
Introduction
ele
All ustrious pmWasabi-C is a mammalian expression vector with a multiple
cloning site for fusions to the C terminus of mWasabi, also suitable for
ele
expression of mWasabi alone in mammalian cells. All ustrious mWasabi is
a monomeric green fluorescent protein that can be easily detected using
standard GFP filter sets. mWasabi may be used as a direct replacement for
EGFP or other GFPs for superior performance, and may be co-imaged with
blue and red fluorescent labels without substantial bleed-through.
• About 2-fold brighter than EGFP
•
•
•
•
•
•
Similar photostability to EGFP
Uses standard filter sets
Can be co-imaged with blue and red FPs or dyes
Mammalian expression vector ready to transfect your favorite cells
Low sensitivity to acidic pH (fluorescence pKa=4.3)
True monomer that will not aggregate or cause nonspecific
interactions
Reconstitution
10 µg provided in lyophilized powder form. Reconstitute with 10 µL of
nuclease-free water for a final concentration of 1 µg/µL.
Source
Storage
Engineered variant of mTFP1, originally derived from Clavularia sp. coral.
Store at -20°C or at -80°C for long-term preservation.
Human CMV Immediate-Early
Promoter (CMV Promoter)........1-595
mWasabi.....................................613-1320
PCMV
pUC Ori
Multiple Cloning Sites (MCS)....1321-1414
SV40 PolyA Signal.....................1560-1610
bla Promoter...............................2149-2253
pmWasabi-C
Ampicillin Resistance Gene......2238-3098
mWasabi
3971bp
pUC Origin..................................3247-3889
Upstream Sequencing Primer:
AMPR
Universal CMV Promoter Primer
Downstream Sequencing Primer:
BLA
MCS
SV40
Poly A
SV40 Primer:GCTTT ATTTG TGAAA TTTGT GATGC TATTG C
Human CMV Immediate Early Promoter
481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT
541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCTCTA
NheI
601 GACTGGATCC TCGAATTCGG TACCTAAAGC TTGCGGCCGC CTCGAGGCCA CCATGGTCAG
BamHI
EcoRI KpnI
HindIII NotI
XhoI Kozak Start
codon of mTFP1
661 CAAGGGCGAG GAGACCACAA TGGGCGTAAT CAAGCCCGAC ATGAAGATCA AGCTGAAGAT
1321 GGCCCGCAAC TCCACCGACG GCATGGACGA GCTGTACAAG TAAGCATGCA GATCCACCGG
Stop codon of mTFP1
References: Ai H, Olenych SG, Wong P, Davidson MW, Campbell RE. Hue-shifted monomeric variants Clavularia cyan fluorescent protein: identification
of the molecular determinants of color and applications in fluorescence imaging. BMC Biology. 2008 Mar; 6:13. Shaner NC, Patterson GH, Davidson MW.
Advances in fluorescent protein technology. J Cell Sci. 2007 Dec 15;120(Pt 24):4247-60. Ai HW, Hazelwood KL, Davidson MW, Campbell RE.
Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Nature Methods. 2008 5(5): 401-03. Ai HW, Henderson JN, Remington SJ,
Campbell RE. Directed evolution of a monomeric, bright, and photostable version of Clavularia cyan fluorescent protein: structural characterization and
applications in fluorescence imaging. Biochem J. 2006. Shaner NC, Steinbach PA, Tsien RY. A guide to choosing fluorescent proteins. Nat Methods.
2005 2(12):905-09
Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645
Fax: (858) 587-6692 [email protected] Copyright ©2008-2010
Allele Biotechnology & Pharmaceuticals, Inc.
allelebiotech.com
SEQUENCE
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
TAGTTATTAA
CGTTACATAA
GACGTCAATA
ATGGGTGGAG
AAGTACGCCC
CATGACCTTA
CATGGTGATG
ATTTCCAAGT
GGACTTTCCA
ACGGTGGGAG
CCGGTCGCCA
ATGAAGATCA
GAGGGCGAGG
GCCCCCCTGC
TTCACCAAGT
TCTTGGGAGC
TCCATGGAGG
AACGGCCCCG
GTGCGCGACG
CACCACCGCG
TATCACTTTG
ACCGTTTACG
TCCGGACTCA
CGCTAACTAG
CCACATTTGT
AACATAAAAT
AATAAAGCAA
GTGGTTTGTC
GCGGGTGTGG
CCTTTCGCTT
AATCGGGGGC
CTTGATTAGG
TTGACGTTGG
AACCCTATCT
TTAAAAAATG
ACAATTTAGG
AAATACATTC
ATTGAAAAAG
CGGCATTTTG
AAGATCAGTT
TTGAGAGTTT
GTGGCGCGGT
ATTCTCAGAA
TGACAGTAAG
TACTTCTGAC
ATCATGTAAC
AGCGTGACAC
AACTACTTAC
CAGGACCACT
CCGGTGAGCG
GTATCGTAGT
TCGCTGAGAT
ATATACTTTA
TTTTTGATAA
ACCCCGTAGA
GCTTGCAAAC
CAACTCTTTT
TAGTGTAGCC
CTCTGCTAAT
TGGACTCAAG
GCACACAGCC
TATGAGAAAG
GGGTCGGAAC
GTCCTGTCGG
GGCGGAGCCT
GGCCTTTTGC
CCGCCATGCA
TAGTAATCAA
CTTACGGTAA
ATGACGTATG
TATTTACGGT
CCTATTGACG
TGGGACTTTC
CGGTTTTGGC
CTCCACCCCA
AAATGTCGTA
GTCTATATAA
CCATGGTGAG
AGCTGAAGAT
GCAAGCCCTA
CCTTCTCCTA
ACCCCGACGA
GCACCATGAC
AGGACTCCTT
TGATGCAGAA
GCGTGCTGAA
TTGACTTCAA
TGGACCACCG
AGATCGCCGT
GATCTCGAGT
CTGAGCATGC
AGAGGTTTTA
GAATGCAATT
TAGCATCACA
CAAACTCATC
TGGTTACGCG
TCTTCCCTTC
TCCCTTTAGG
GTGATGGTTC
AGTCCACGTT
CGGTCTATTC
AGCTGATTTA
TGGCACTTTT
AAATATGTAT
GAAGAGTATG
CCTTCCTGTT
GGGTGCACGA
TCGCCCCGAA
ATTATCCCGT
TGACTTGGTT
AGAATTATGC
AACGATCGGA
TCGCCTTGAT
CACGATGCCT
TCTAGCTTCC
TCTGCGCTCG
TGGGTCTCGC
TATCTACACG
AGGTGCCTCA
GATTGATTTA
TCTCATGACC
AAAGATCAAA
AAAAAAACCA
TCCGAAGGTA
GTAGTTAGGC
CCTGTTACCA
ACGATAGTTA
CAGCTTGGAG
CGCCACGCTT
AGGAGAGCGC
GTTTCGCCAC
ATGGAAAAAC
TCACATGTTC
T
TTACGGGGTC
ATGGCCCGCC
TTCCCATAGT
AAACTGCCCA
TCAATGACGG
CTACTTGGCA
AGTACATCAA
TTGACGTCAA
ACAACTCCGC
GCAGAGCTGG
CAAGGGCGAG
GGAGGGCAAC
CGACGGCACC
CGACATTCTG
CATCCCCAAC
CTTCGAGGAC
CATCTACGAG
GGAGACCACC
GGGCGACGTC
GACCATCTAC
CATCGAGATC
GGCCCGCAAC
CGGATCCTCG
CGATCCACCG
CTTGCTTTAA
GTTGTTGTTA
AATTTCACAA
AATGTATCTT
CAGCGTGACC
CTTTCTCGCC
GTTCCGATTT
ACGTAGTGGG
CTTTAATAGT
TTTTGATTTA
ACAAAAATTT
CGGGGAAATG
CCGCTCATGA
AGTATTCAAC
TTTGCTCACC
GTGGGTTACA
GAACGTTTTC
ATTGACGCCG
GAGTACTCAC
AGTGCTGCCA
GGACCGAAGG
CGTTGGGAAC
GTAGCAATGG
CGGCAACAAT
GCCCTTCCGG
GGTATCATTG
ACGGGGAGTC
CTGATTAAGC
AAACTTCATT
AAAATCCCTT
GGATCTTCTT
CCGCTACCAG
ACTGGCTTCA
CACCACTTCA
GTGGCTGCTG
CCGGATAAGG
CGAACGACCT
CCCGAAGGGA
ACGAGGGAGC
CTCTGACTTG
GCCAGCAACG
TTTCCTGCGT
ATTAGTTCAT
TGGCTGACCG
AACGCCAATA
CTTGGCAGTA
TAAATGGCCC
GTACATCTAC
TGGGCGTGGA
TGGGAGTTTG
CCCATTGACG
TTTAGTGAAC
GAGACCACAA
GTGAATGGCC
AACACCATCA
ACCACCGCGT
TACTTCAAGC
AAGGGCATCG
ATACACCTCA
GGCTGGGACG
AAGATGAAGC
AGGGCCAAGA
CTGAACCACG
TCCACCGACG
AATTCGGTAC
GATCTAGATA
AAAACCTCCC
ACTTGTTTAT
ATAAAGCATT
AACGCGTCCT
GCTACACTTG
ACGTTCGCCG
AGTGCTTTAC
CCATCGCCCT
GGACTCTTGT
TAAGGGATTT
AACGCGAATT
TGCGCGGAAC
GACAATAACC
ATTTCCGTGT
CAGAAACGCT
TCGAACTGGA
CAATGATGAG
GGCAAGAGCA
CAGTCACAGA
TAACCATGAG
AGCTAACCGC
CGGAGCTGAA
CAACAACGTT
TAATAGACTG
CTGGCTGGTT
CAGCACTGGG
AGGCAACTAT
ATTGGTAACT
TTTAATTTAA
AACGTGAGTT
GAGATCCTTT
CGGTGGTTTG
GCAGAGCGCA
AGAACTCTGT
CCAGTGGCGA
CGCAGCGGTC
ACACCGAACT
GAAAGGCGGA
TTCCAGGGGG
AGCGTCGATT
CGGCCTTTTT
TATCCCCTGA
AGCCCATATA
CCCAACGACC
GGGACTTTCC
CATCAAGTGT
GCCTGGCATT
GTATTAGTCA
TAGCGGTTTG
TTTTGGCACC
CAAATGGGCG
CGTCAGATCC
TGGGCGTAAT
ACGCCTTCGT
ACCTGGAGGT
TCAGTTACGG
AGTCCTTCCC
TGAAGGTGAA
AGGGCGAGAA
CCTCCACCGA
TGCTGCTGGA
AGGCGGTGAA
ACAAGGACTA
GCATGGACGA
CCTCTAGACA
ACTGATCATA
ACACCTCCCC
TGCAGCTTAT
TTTTTCACTG
GTAGCGGCGC
CCAGCGCCCT
GCTTTCCCCG
GGCACCTCGA
GATAGACGGT
TCCAAACTGG
TGCCGATTTC
TTAACAAAAT
CCCTATTTGT
CTGATAAATG
CGCCCTTATT
GGTGAAAGTA
TCTCAACAGC
CACTTTTAAA
ACTCGGTCGC
AAAGCATCTT
TGATAACACT
TTTTTTGCAC
TGAAGCCATA
GCGCAAACTA
GATGGAGGCG
TATTGCTGAT
GCCAGATGGT
GGATGAACGA
GTCAGACCAA
AAGGATCTAG
TTCGTTCCAC
TTTTCTGCGC
TTTGCCGGAT
GATACCAAAT
AGCACCGCCT
TAAGTCGTGT
GGGCTGAACG
GAGATACCTA
CAGGTATCCG
AAACGCCTGG
TTTGTGATGC
ACGGTTCCTG
TTCTGTGGAT
TGGAGTTCCG
CCCGCCCATT
ATTGACGTCA
ATCATATGCC
ATGCCCAGTA
TCGCTATTAC
ACTCACGGGG
AAAATCAACG
GTAGGCGTGT
GCTAGCGCTA
CAAGCCCGAC
GATCGAGGGC
GAAGGAGGGA
CAACAGGGCC
CGAGGGCTAC
GTCCGACATC
CTTCCCCCCC
GAGGATGTAC
GGGCGGCGGC
GCTGCCCGAC
CAACAAGGTG
GCTGTACAAG
AGCTTGCGGC
ATCAGCCATA
CTGAACCTGA
AATGGTTACA
CATTCTAGTT
ATTAAGCGCG
AGCGCCCGCT
TCAAGCTCTA
CCCCAAAAAA
TTTTCGCCCT
AACAACACTC
GGCCTATTGG
ATTAACGCTT
TTATTTTTCT
CTTCAATAAT
CCCTTTTTTG
AAAGATGCTG
GGTAAGATCC
GTTCTGCTAT
CGCATACACT
ACGGATGGCA
GCGGCCAACT
AACATGGGGG
CCAAACGACG
TTAACTGGCG
GATAAAGTTG
AAATCTGGAG
AAGCCCTCCC
AATAGACAGA
GTTTACTCAT
GTGAAGATCC
TGAGCGTCAG
GTAATCTGCT
CAAGAGCTAC
ACTGTTCTTC
ACATACCTCG
CTTACCGGGT
GGGGGTTCGT
CAGCGTGAGC
GTAAGCGGCA
TATCTTTATA
TCGTCAGGGG
GCCTTTTGCT
AACCGTATTA
Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645
Fax: (858) 587-6692 [email protected] Copyright ©2008-2010
Allele Biotechnology & Pharmaceuticals, Inc.
Enzyme
AatII
Acc113I
Acc16I
Acc65I
AccB1I
AccB7I
AccBSI
AccIII
AcsI
AcyI
AfeI
AflIII
AgeI
AhdI
Alw21I
Alw44I
AlwNI
Ama87I
Aor51HI
ApaLI
ApoI
AseI
AsnI
Asp700I
Asp718I
AspEI
AspHI
AvaI
AviII
BalI
BamHI
BanI
BanII
BbiII
BbuI
Bbv12I
BcgI
BclI
BcoI
BglI
BglII
BlpI
BpmI
Bpu1102I
BsaAI
BsaBI
BsaHI
BsaI
BsaMI
BsaOI
BsaWI
Bse118I
Bse8I
BseAI
BseRI
Bsh1285I
Bsh1365I
BshNI
BsiEI
BsiHKAI
BsiI
BsiMI
BsmI
BsoBI
Bsp13I
Bsp1407I
Bsp143II
Bsp1720I
Bsp19I
BspCI
BspEI
BspHI
BspLU11I
BsrBI
BsrBRI
BsrDI
BsrFI
BsrGI
BssAI
BssSI
BssT1I
BstD102I
BstDSI
BstEII
BstH2I
BstI
BstMCI
BstPI
BstSFI
BstSNI
BstX2I
Cuts
5
1
1
1
5
1
2
1
4
7
1
2
1
1
3
2
1
2
1
2
4
2
2
1
1
1
3
2
1
1
1
5
2
7
1
3
1
1
2
5
1
1
3
1
3
1
7
3
2
5
6
4
1
1
2
5
1
5
5
3
2
1
2
2
1
1
4
1
3
1
1
2
1
2
1
2
4
1
4
2
3
2
4
1
4
1
5
1
5
1
11
BstXI
BstYI
1
11
BstZI
CciNI
CelII
Cfr10I
CfrI
DraI
1
1
1
4
4
4
Positions
125 178 261 447 1109
2544
2802
1356
465 745 1356 1841 3072
1052
1741 2186
1321
1350 1580 2065 2076
122 175 258 444 1058 1106 2485
596
1652 3913
600
3025
2357 2442 3603
2353 3599
3504
889 1334
596
2353 3599
1350 1580 2065 2076
7 2850
7 2850
2425
1356
3025
2357 2442 3603
889 1334
2802
698
1342
465 745 1356 1841 3072
783 1811
122 175 258 444 1058 1106 2485
1399
2357 2442 3603
2509
1423
889 1334
90 212 283 1180 2907
1330
1391
768 1131 2940
1391
340 1080 1882
1428
122 175 258 444 1058 1106 2485
636 1047 2958
1516 1615
605 1380 2507 2656 3579
600 1321 1407 2729 3560 3707
600 1048 1777 2940
1428
1321
633 972
605 1380 2507 2656 3579
1428
465 745 1356 1841 3072
605 1380 2507 2656 3579
2357 2442 3603
2361 3745
1321
1516 1615
889 1334
1321
1313
598 1727 1735 3673
1391
360 611 962
2656
1321
2185 3193
3913
1741 2186
1428
2789 2971
600 1048 1777 2940
1313
600 1048 1777 2940
2361 3745
360 611 962
1741 2186
360 611 962 1277
1257
598 1727 1735 3673
1342
605 1380 2507 2656 3579
1257
1167 1659 2779 3457 3648
340
585 1226 1330 1342 1410 2378
2395 3163 3175 3261 3272
762
585 1226 1330 1342 1410 2378
2395 3163 3175 3261 3272
1377
1377
1391
600 1048 1777 2940
696 1137 1377 2632
1468 2447 3139 3158
Sequence
gacgt/c
agt/act
tgc/gca
g/gtacc
g/gyrcc
ccannnn/ntgg
gagcgg
t/ccgga
r/aatty
gr/cgyc
agc/gct
a/crygt
a/ccggt
gacnnn/nngtc
gwgcw/c
g/tgcac
cagnnn/ctg
c/ycgrg
agc/gct
g/tgcac
r/aatty
at/taat
at/taat
gaann/nnttc
g/gtacc
gacnnn/nngtc
gwgcw/c
c/ycgrg
tgc/gca
tgg/cca
g/gatcc
g/gyrcc
grgcy/c
gr/cgyc
gcatg/c
gwgcw/c
cgannnnnntgc
t/gatca
c/ycgrg
gccnnnn/nggc
a/gatct
gc/tnagc
ctggag
gc/tnagc
yac/gtr
gatnn/nnatc
gr/cgyc
ggtctc
gaatgc
cgry/cg
w/ccggw
r/ccggy
gatnn/nnatc
t/ccgga
gaggag
cgry/cg
gatnn/nnatc
g/gyrcc
cgry/cg
gwgcw/c
ctcgtg
t/ccgga
gaatgc
c/ycgrg
t/ccgga
t/gtaca
rgcgc/y
gc/tnagc
c/catgg
cgat/cg
t/ccgga
t/catga
a/catgt
gagcgg
gatnn/nnatc
gcaatg
r/ccggy
t/gtaca
r/ccggy
ctcgtg
c/cwwgg
gagcgg
c/crygg
g/gtnacc
rgcgc/y
g/gatcc
cgry/cg
g/gtnacc
c/tryag
tac/gta
r/gatcy
ccannnnn/ntgg
r/gatcy
c/ggccg
gc/ggccgc
gc/tnagc
r/ccggy
y/ggccr
ttt/aaa
DraII
DraIII
DrdI
DsaI
EaeI
EagI
Eam1104I
Eam1105I
EarI
EclHKI
EclXI
Eco105I
Eco130I
Eco24I
Eco255I
Eco31I
Eco47III
Eco52I
Eco57I
Eco64I
Eco88I
Eco91I
EcoO109I
EcoO65I
EcoRI
EcoT14I
EcoT22I
ErhI
Esp1396I
FauNDI
FbaI
FriOI
FspI
GsuI
HaeII
Hin1I
HincII
HindII
HindIII
HpaI
Hsp92I
Kpn2I
KpnI
Ksp22I
Ksp632I
MamI
MfeI
MflI
1
2
2
4
4
1
1
1
1
1
1
1
3
2
1
3
1
1
4
5
2
1
1
1
1
3
1
3
1
1
1
2
1
3
4
7
2
2
1
1
7
1
1
1
1
1
1
11
MluI
MluNI
Mph1103I
MroI
MroNI
MscI
MslI
Msp17I
MspA1I
MunI
Mva1269I
NaeI
NcoI
NdeI
NgoAIV
NgoMI
NheI
NotI
NsiI
NspBII
NspI
PaeI
PaeR7I
PflMI
PinAI
Ple19I
Ppu10I
PshBI
Psp1406I
PspEI
PstNHI
PvuI
RcaI
ScaI
SfcI
Sfr274I
SnaBI
SphI
SspBI
SspI
StyI
Van91I
VneI
VspI
XbaI
XcmI
XhoI
XhoII
1
1
1
1
1
1
6
7
3
1
2
1
3
1
1
1
1
1
1
3
2
1
1
1
1
1
1
2
2
1
1
1
2
1
5
1
1
1
1
2
3
1
2
2
2
1
1
11
XmaIII
XmnI
Zsp2I
1
1
1
allelebiotech.com
836
1209 1885
1929 3811
360 611 962 1277
696 1137 1377 2632
1377
2236
3025
2236
3025
1377
340
360 611 962
783 1811
2544
636 1047 2958
596
1377
678 1101 2343 3391
465 745 1356 1841 3072
889 1334
1257
836
1257
1350
360 611 962
3970
360 611 962
1052
234
1423
783 1811
2802
768 1131 2940
598 1727 1735 3673
122 175 258 444 1058 1106 2485
1152 1529
1152 1529
1370
1529
122 175 258 444 1058 1106 2485
1321
1360
1423
2236
1428
1516
585 1226 1330 1342 1410 2378
2395 3163 3175 3261 3272
1652
698
3970
1321
1777
698
365 613 961 2254 2613 2772
122 175 258 444 1058 1106 2485
2389 3330 3575
1516
1516 1615
1779
360 611 962
234
1777
1777
591
1377
3970
2389 3330 3575
1399 3917
1399
1334
1052
600
2656
3966
7 2850
2423 2796
1257
591
2656
2185 3193
2544
1167 1659 2779 3457 3648
1334
340
1399
1313
2090 2220
360 611 962
1052
2353 3599
7 2850
1363 1413
1299
1334
585 1226 1330 1342 1410 2378
2395 3163 3175 3261 3272
1377
2425
3970
rg/gnccy
cacnnn/gtg
gacnnnn/nngtc
c/crygg
y/ggccr
c/ggccg
ctcttc
gacnnn/nngtc
ctcttc
gacnnn/nngtc
c/ggccg
tac/gta
c/cwwgg
grgcy/c
agt/act
ggtctc
agc/gct
c/ggccg
ctgaag
g/gyrcc
c/ycgrg
g/gtnacc
rg/gnccy
g/gtnacc
g/aattc
c/cwwgg
atgca/t
c/cwwgg
ccannnn/ntgg
ca/tatg
t/gatca
grgcy/c
tgc/gca
ctggag
rgcgc/y
gr/cgyc
gty/rac
gty/rac
a/agctt
gtt/aac
gr/cgyc
t/ccgga
ggtac/c
t/gatca
ctcttc
gatnn/nnatc
c/aattg
r/gatcy
a/cgcgt
tgg/cca
atgca/t
t/ccgga
g/ccggc
tgg/cca
caynn/nnrtg
gr/cgyc
cmg/ckg
c/aattg
gaatgc
gcc/ggc
c/catgg
ca/tatg
g/ccggc
g/ccggc
g/ctagc
gc/ggccgc
atgca/t
cmg/ckg
rcatg/y
gcatg/c
c/tcgag
ccannnn/ntgg
a/ccggt
cgat/cg
a/tgcat
at/taat
aa/cgtt
g/gtnacc
g/ctagc
cgat/cg
t/catga
agt/act
c/tryag
c/tcgag
tac/gta
gcatg/c
t/gtaca
aat/att
c/cwwgg
ccannnn/ntgg
g/tgcac
at/taat
t/ctaga
ccannnnn/nnnntgg
c/tcgag
r/gatcy
c/ggccg
gaann/nnttc
atgca/t
Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645
Fax: (858) 587-6692 [email protected] Copyright ©2008-2010
Allele Biotechnology & Pharmaceuticals, Inc.
allelebiotech.com
The following enzymes do not cut:
AatI, AccI, AclNI, AflII, AocI, ApaI, AscI, AspI, AtsI, AvrII, BanIII, BbeI, BbrPI, BbsI, Bbv16II, BfrI, BlnI, BpiI, Bpu14I, BpuAI, Bsa29I, BscI, Bse21I, BseCI, BsePI, BsgI, BsiWI, BsmBI, Bsp106I, Bsp119I,
Bsp120I, Bsp68I, BspDI, BspMI, BspTI, BspXI, BssHII, Bst1107I, Bst98I, BstBI, Bsu15I, Bsu36I, Cfr42I, Cfr9I, ClaI, CpoI, Csp45I, CspI, CvnI, Ecl136II, Eco147I, Eco32I, Eco72I, Eco81I, EcoICRI, EcoNI,
EcoRV, EheI, Esp3I, FseI, KasI, KspI, LspI, MspCI, NarI, NruI, NspV, PacI, Pfl23II, PmaCI, Pme55I, PmeI, PmlI, PpuMI, PshAI, Psp124BI, Psp5II, PspAI, PspALI, PspLI, PspOMI, PstI, PvuII, RsrII, SacI,
SacII, SalI, SapI, SbfI, SexAI, SfiI, Sfr303I, SfuI, SgfI, SgrAI, SmaI, SmiI, SpeI, SplI, SrfI, Sse8387I, SseBI, SstI, SstII, StuI, SunI, SwaI, Tth111I, Vha464I, XmaI
Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645
Fax: (858) 587-6692 [email protected] Copyright ©2008-2010
Allele Biotechnology & Pharmaceuticals, Inc.
allelebiotech.com
Related products:
ele
Current All ustious Fluorescent Protein Family Members:
The founding member is mTFP1.
mTFPG3 is a green FP with 3 amino difference from mTFP1. It has a
slightly red-shifted emission spectrum and is 1.5 fold brighter compared to
EGFP. While being very bright, mTFPG3 can be photobleached within ~5
sec, about 30 times faster than EGFP, suitable for certain cell-based
assays that require a bright FP with very short half-life.
mTFP0.7 is a precursor during the evolution of mTFP1. It has
photo-switchable properties like Dronpa that cycles between fluorescent
and nonfluorescent states. It may be developed into components in
PALM/SIM applications.
Basic Vectors
Three vectors are available: pNCS-mWasabi, pmWasabi-N and
pmWasabi-C.
Subcellular Marker Vectors
Twenty six vectors are available.
Vectors in Viral Vectors
All plasmid format vectors in Allele's Phoenix Retroviral vector or HiTiter
Lentiviral Vectors.
IMPORTANT NOTICE TO PURCHASER: This product may be subjects of pending U.S. and foreign patents. Allele Biotech grants academic/government
research institutions a worldwide, non-exclusive, royalty-free, limited license to use this product for non-commercial life science research use only. Such
license specifically prohibits the right to sell or otherwise transfer this product or its derivatives to a third party.
Industry For-Profit Institutions: Institutions that wish to use this product are required to obtain a sub-licensing agreement from Allele Biotech. For more
information please visit Fluorescent Protein Sublicense, or contact us via phone or email.
Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645
Fax: (858) 587-6692 [email protected] Copyright ©2008-2010