IPTG, Brochure
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IPTG (ISOPROPYL SS-D-1-THIOGALACTOPYRANOSIDE)
The chemical IPTG mimics the natural process in single celled organisms where the presence of lactose
stimulates the cell to switch on certain genes that metabolize lactose. IPTG is usually used with a chemical
called X-gal which work together to be visible chemical markers in genetic experiments.
Isopropyl β-D-1-thiogalactopyranoside (IPTG) is a molecular biology reagent. This compound is a molecular
mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon. It therefore induces
protein expression where the gene is under the control of the lac operator.
IPTG, unlike allolactose, is not hydrolyzable by β-galactosidase. Its concentration therefore remains constant
in an experiment. For induction: a sterile, filtered 1 M solution of IPTG is typically added by 1:1000 dilution
into a logarithmically growing bacterial culture. However, a different final concentration of IPTG may also be
used.
Storage and Handling Application
The chemical must be placed in a sealed container
and protected from light. It will degrade in the
presence of moisture and light. IPTG should be
stored at -20°C. IPTG must be warmed to room
temperature prior to exposing to the air to reduce
the amount of water that the chemical will absorb
from the air.
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• IPTG is an effective inducer of protein
expression.
• IPTG helps in stimulating the production of
beta-galactosidase enzyme.
• IPTG is a lactose analog which binds and inhibits
the lac repressor.
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PRODUCT INFORMATION
Product Number
Size available
I-1312
Storage temperature +4°C, long term -20°C
1g
CAS #
367-93-1
5g
Synonym
Isopropyl-d-thiogalactopyranoside
25g
Appearance
White Powder
Molecular formula
C9H18O5S
Molecular weight
> 30 units/mg
Purity
98%
Solubility
Soluble in water and methanol
Melting Point
111°- 113°C
Boiling Point
438°C at 760 mmHg
MDL Number
MFCD00063273
ChemACX
X1007022-5
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