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Base your answers to questions 1 through 6 on the information below and on your
knowledge of biology.
An investigation was carried out to measure the rate of activity of catalase, an
enzyme that breaks down hydrogen peroxide. Five 40-mL solutions of the enzyme at
concentrations of 20%, 40%, 60%, 80%, and 100% were prepared. A filter paper disk
was placed in each enzyme solution. Each soaked disk from the different enzyme concentrations
was then added to different cups containing 30 mL of 1% hydrogen peroxide.
The rate of catalase activity was inferred from measurements of how fast the disks
rose from the bottom to the top of each cup.
The following data were obtained:
400/0-12.1 seconds, 800/0-5.8 seconds, 1000/0-4.1 seconds, 200/0-15.8 seconds, and
600/0- 9.9 seconds.
Directions (1-2): Organize the data by completing the data table, according to
the directions below.
1. Label the second column of the data table with an appropriate heading and record
that label on the y-axis of the graph. [Be sure to include units.] [1]
2
Complete the data table so that the percent enzyme increases from the top to the
bottom of the table. [1]
Enzyme Concentration
(percent)
8Species A
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Species B
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Day
D
D
D
'f. Based on the
daily counts, on which day did it first become evident that one species
was better adapted than the other species for survival in the environment provided? [1]
D
5- The difference in the population sizes on the fifth day most likely resulted from
(1) temperature changes
(2) variations in light intensity
(3) competition between species
(4) the buildup of nitrogen gas
D
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Base your answers to questions.'
and on your knowledge of biology,
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information and data table below
A biology student performed an experiment to determine which of two species of
single-celled organisms would survive best when cultured together in a certain environment. The student placed 10 organisms of each species into a large test tube,
Throughout the experiment, the test tube was maintained at 30°C. After the test tube
was set up, the population of each species was determined each day for 5 days, The data
collected are shown in the table below.
Data Table
Population
Day
Species A
Species B
1
10
10
2
16
16
3
32
32
4
48
12
5
60
4
Directions (I .•••5 J: Using the information in the data table, construct a line graph on
the grid on the next page, following the directions below.
l : Mark an appropriate scale on each labeled axis. [1]
d-.
Plot the data for species A on the grid. Surround each point with a small circle and connect the points. [1]
Example:
..3.
~
Plot the data for species B on the grid. Surround each point with a small triangle and
connect the points.
[t]
Example:
~
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For Teacher
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·
Directions (3-4): Using the information in the data table, construct a line graph on the
grid provided, following the directions below.
3.
Mark an appropriate scale on each axis. [1]
4
Plot the data from your data table. Surround each point with a small circle and
connect the points. [1]
r>.
Example:
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5. State one valid conclusion that relates enzyme concentration to reaction rate. [1]
6. State two factors which would cause a decrease in the activity of catalase. [1]
Base your answers to questions 1 through 6 on the information below and on your
knowledge of biology.
An investigation was carried out to measure the rate of activity of catalase, an
enzyme that breaks down hydrogen peroxide. Five 40-mL solutions of the enzyme at
concentrations of 20%, 40%, 60%, 80%, and 100% were prepared. A filter paper disk
was placed in each enzyme solution. Each soaked disk from the different enzyme concentrations
was then added to different cups containing 30 mL of 1% hydrogen peroxide.
The rate of catalase activity was inferred from measurements of how fast the disks
rose from the bottom to the top of each cup.
The following data were obtained:
400/0-12.1 seconds, 800/0-5.8 seconds, 1000/0-4.1 seconds, 200/0-15.8 seconds, and
600/0- 9.9 seconds.
Directions (1-2): Organize the data by completing the data table, according to
the directions below.
1. Label the second column of the data table with an appropriate heading and record
that label on the y-axis of the graph. [Be sure to include units.] [1]
2
Complete the data table so that the percent enzyme increases from the top to the
bottom of the table. [1]
Enzyme Concentration
(percent)
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100
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Directions (3-4): Using the information in the data table, construct a line graph on the
grid provided, following the directions below.
3.
Mark an appropriate scale on each axis. [1]
4
Plot the data from your data table. Surround each point with a small circle and
connect the points. [1]
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Example:
/10
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5. State one valid conclusion that relates enzyme concentration to reaction rate. [1]
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6. State two factors which would cause a decrease in the activity of catalase. [1]
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~. Based on the daily counts, on which day did it first become evident that one species
was better adapted than the other species for survival in the environment provided? [1]
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5. The
difference in the population
(1) temperature
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sizes on the fifth day most likely resulted from
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(2) variations in light intensity
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(4) the buildup of nitrogen
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