Problem:
What macromolecules are in my
food?
Objective: To use qualitative tests to identify
macromolecules
Do Now 12/18
Name 3 safety rules.
1.
Do Research: Fill in the table below:
Macromolecule
Monomer (subunit)
Function
Examples of food
Hypothesis: Present → +
Solutions
Milk
Orange Juice
Turkey (Baby
food)
Diet Soda
Egg Whites
Starch Test
(Gram’s Iodine)
Not Present → -
Simple Sugar Test
(Benedict’s Solution)
Protein Test
(Biuret Reagent)
Lipid Test
(Paper Bag)
Before
After
Safety Rules:
1. Wear __goggles__________,
___gloves__________, and an
____apron________.
2. Do not __eat________ /
_____drink______ any chemical
or solutions.
3. Do not ___point__________ the
heated test tube towards
your face.
4. Use a ___clamp______________
to hold the heated test tube.
Procedure:
(SIMPLE SUGAR - BENEDICT’S SOLUTION)
1.
Put _____1 dropper full________ of solution in each test tube.
2. Add __3 drops________ of Benedict’s solution to EACH test tube.
3. Put test tubes in “hot water” for _____2 minutes__________ .
4. Record color change.
Procedure: STARCH TEST - GRAM’S IODINE
1.
Put a _______________ of solution in
each test tube.
2. Add _______ of Gram’s
EACH solution
3. Record the color change.
Iodine to
Procedure: PROTEIN TEST - BIURET REAGENT
**corrosive and will burn your skin**
1.
Put a ___dropper full_______________ of solution in each
test tube.
2. Add ___3 drops_______ of Biuret Reagent to EACH
solution.
3. Add test tube to “hot” water for 2 minutes.
4. Record the color change.
Lipid Test: Brown Paper Bag Test
translucent
not translucent
1. Place 2 drops of each
solution on the paper bag.
2. Wait 15 minutes.
3. Check to see if the spot is
translucent.
Results: Present → +
Not Present → -
Title: ________________________________
Solutions
Milk
Orange Juice
Turkey (Baby
food)
Diet Soda
Egg Whites
Starch Test
(Gram’s Iodine)
Simple Sugar Test
(Benedict’s Solution)
Protein Test
(Biuret Reagent)
Lipid Test
(Paper Bag)
Conclusion
I accept/reject my hypothesis for milk because ……
I accept/reject my hypothesis for orange juice because..
I accept/reject my hypothesis for egg whites because..
I accept/reject my hypothesis for turkey and gravy because..
I accept/reject my hypothesis for diet soda because...
S
Conclusion Paragraph
In this lab we,..... (describe the lab)............
We followed the following safety rules: 1. _______________
2. _____________, and 3. _______________.
I learned that _______________________________________.
Protein is important because it ___________________________.
Carbohydrates are important because it _____________________.
Lipids are important because it ______________________.
I want to know __________________________________________.
Controlled Experiment Setup
Controlled Experiment Setup
Which part of the experiment
is it?
Control Group
Mrs. Bicksler’s test results on
our notes.
Experimental Group
Independent Variable
Dependent Variable
Why? Explanation of your
choice.