HAEMATOLOGY AND BLOOD TRANSFUSION
USER HANDBOOK
Table of Contents
Table of Contents ..................................................................................................................................................................................................... 2
General Information .................................................................................................................................................................................................. 3
Location....................................................................................................................................................................................................................................... 3
Contact Numbers and Working Hours ......................................................................................................................................................................................... 3
Near Patient Testing facilities (Point of Care Laboratories) ......................................................................................................................................................... 4
Phlebotomy Services ................................................................................................................................................................................................................... 5
Urgent Requests ......................................................................................................................................................................................................................... 5
Out-of-Hours Service (after hours Monday - Saturday, Sunday and Public Holidays). ................................................................................................................. 5
Making a complaint to the Laboratory .......................................................................................................................................................................................... 5
Medical Staff ............................................................................................................................................................................................................................... 6
Completion of the request form ................................................................................................................................................................................................... 7
Sample Collection (phlebotomy) .................................................................................................................................................................................................. 7
Vacutainer™ Guide ..................................................................................................................................................................................................................... 8
Sample Labelling ......................................................................................................................................................................................................................... 9
Sample Transport to the Laboratory ............................................................................................................................................................................................ 9
Special Handling Requirements .................................................................................................................................................................................................. 9
Routine Haematology (page 1 of 3) ........................................................................................................................................................................ 10
Blood Transfusion (page 1 of 5) ............................................................................................................................................................................. 13
Routine Coagulation (page 1 of 2) .......................................................................................................................................................................... 19
Haemostasis (page 1 of 6) ...................................................................................................................................................................................... 21
Special Haematology (page 1 of 3) ........................................................................................................................................................................ 27
Haemoglobinopathy Genetics (page 1 of 1) .......................................................................................................................................................... 30
Flow Cytometry (HIV and Immunophenotyping) (page 1 of 5) ................................................................................................................................ 31
Specialist Integrated Haematological Malignancy Diagnostic (SIHMDS) (page 1 of 5) .......................................................................................... 36
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Page 2 of 40
Review Date: 10th October 2016
General Information
Location
The Haematology and Blood Transfusion laboratories are located at 60 Whitfield St London W1T 4EU. Samples are sent from the main hospital to Specimen
Reception via the ‘Pod’ system and sample deliveries made by courier or from other sites are made via a rear building entrance in Howland Mews. Near Patient
Testing facilities for Haematology/Oncology patients are located on the Lower Ground Floor of the MacMillan Cancer Centre on Huntley Street.
Haemoglobinopathy Genetics laboratories are located at Ground Floor, 307 Euston Road, London. NW1
Contact Numbers and Working Hours
General Manager
Debbie Mann
0203 447 2968 or x72968
9.00 – 17.15 Monday - Friday
Training and Development Manager
0203 447 9887 or x79887
9.00 – 17.15 Monday - Friday
0203 447 9887 or x79887
9.00 – 17.15 Monday - Friday
0203 447 8533 or x78533
9.00 – 17.15 Monday - Friday
Stefan Kunka
Quality Compliance Coordinator
Susan Crouch
Health & Safety Officer
Norma McQueen
9.00 – 19.15 Monday – Friday
9.00 – 13.30 Saturday
9.00 – 13.00 Sunday
Routine Haematology & Coagulation
Departmental Lead: Billy Janda
Routine Haematology & General Enquiries
Routine Coagulation
Haemostasis Laboratory (Specialised Coagulation)
0203 447 8961 or 78961
0203 447 8525 or x78525
0203 447 8547 or x78547
Out-of-hours Bleep 7060
0203 447 8549/8529 or x78549/78529
Departmental Lead: Pamela Carr
0203 447 8545 or x78545
Flow Cytometry (HIV and Immunophenotyping)
0203 447 9603 or 79603
9.00 – 17.15 Monday - Friday
9.00 – 17.15 Monday – Friday
9.00 – 17.15 Monday – Friday
Departmental Lead: Naina Chavda
0203 447 8541 or x 78541
A limited Leukaemia Immunophenotyping service is available out-of-hours (subject to authorisation by a Haematology Consultant).
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Page 3 of 40
Review Date: 10th October 2016
Blood Transfusion Laboratory
0203 447 8522/8523 or x78522/78523
Out-of-hours Bleep 7060
Service Manager: Jenny Berryman
Acting Laboratory Manager: Ian Longair
Blood Transfusion Quality Manager: Lubna Awas
9.00 – 19.15 Monday – Friday
9.00 – 13.30 Saturday
9.00 – 13.00 Sunday
0203 447 8522/8523 or x78522/78523
0203 447 8521 or ext 78521
0203 447 8521 or ext 78521
9.00 – 17.15 Monday - Friday
9.00 – 17.15 Monday – Friday
9.00 – 17.15 Monday – Friday
MAJOR HAEMORRHAGE
For a Major Haemorrhage at any time: dial telephone extension 2222 (or extension 32222 from the Heart Hospital) and state "Major Haemorrhage" to the
switchboard operator to activate the UCLH Trust’s Major Haemorrhage Protocol. In the event of a telephone network failure: dial the Analogue Direct
Telephone Line to Blood Transfusion *020 7 631 0267, or Emergency Mobile *0771 851 2912.
Note, these telephone numbers are for emergency use only: any use of these lines for routine communication will be reported as a clinical incident.
Special Haematology (Haemoglobinopathies)
0203 447 8533 or x78533
9.00 – 17.15 Monday – Friday
Departmental Lead: Norma McQueen
Haemoglobinopathy Genetics
Friday
9.00 – 17.00 Monday –
0203 447 9458 or x79458 or 0204 447 5565 or 75565
Scientific and Clinical Departmental Lead: Dr Mary Petrou
Operational Lead: Kristian Rennie
See separate User Handbook for Haemoglobinopathy test information - Haemoglobinopathy Genetics User Handbook (HAEM-PD-HGUserHbook)
;
SIHMDS Specimen Reception (for all Haematological Malignancy samples) 0203 447 8518 or 78518
9.00 – 17.15 Monday – Friday
Samples are forwarded from the SIHMDS specimen reception to Flow Cytometry, Histology and external reference laboratories.
Near Patient Testing facilities (Point of Care Laboratories)
Haematology Point of Care Laboratories provide Full Blood Count results exclusively for Haematology/Oncology outpatients receiving chemotherapy or
attending clinic appointments and for the Accident and Emergency Department.
MacMillan Cancer Centre (Huntley Street), Lower Ground Floor
0203 447 6855 or extension 76855
Accident and Emergency Laboratory – contact main laboratory 0203 447 8961 or extension 78961
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
08.00 – 17.00 Monday-Friday
08.00 – 20.00 Monday – Friday.
Page 4 of 40
Review Date: 10th October 2016
Phlebotomy Services
For phlebotomy opening times please refer to INSIGHT phlebotomy page via the following link:
http://insight/departments/MedicineBoard/Pathology/HaematologyPathology/Phlebotomy/Pages/default.aspx
Urgent Requests
Please contact the relevant laboratory during routine working hours, or Bleep 7060 outside routine working hours to alert laboratory staff of samples en route for
urgent processing.
Out-of-Hours Service (after hours Monday - Saturday, Sunday and Public Holidays).
The Haematology Laboratory provides a 7 day / 24 hour restricted service outside of routine hours: a Biomedical Scientist can be contacted via the relevant
Routine Haematology, Routine Coagulation or Blood Transfusion laboratory telephone extension number until 19.15 (see above), or via Bleep 7060 at any time.
The following services are available out of routine working hours:
 Full Blood Count (FBC), Erythrocyte Sedimentation Rate (ESR), Blood Film Examination.
 Clotting Screen / Fibrinogen Assay / D-Dimers
 Group and Antibody Screen for Pre-op/Crossmatch
 Crossmatch
 Pre-op sickle testing
Bleep 7060 to discuss any urgent requests for any services that are not usually available out of routine working hours.
Making a complaint to the Laboratory
If you would like to make a complaint relating to a specific test please contact the relevant section lead, Blood Transfusion Quality Manager or Haematology
Compliance Coordinator.
Alternatively, please complete the online Datix incident reporting procedure available on the intranet and we will investigate your complaint.
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Page 5 of 40
Review Date: 10th October 2016
Medical Staff
Consultants (Speciality)
Dr Marie Scully- Lead Clinician
(TTP, Haematology & Transfusion)
Dr Kirit Ardeshna (Lymphoma)
Dr Hannah Cohen- via secretary (Haemostasis)
Dr Bernard Davis (Joint red cell unit)
Dr Shirley D’Sa
(Myeloma &Waldenstroms macroglobulinaemia)
Dr Victoria Grandage
(Children & young peoples cancer services)
Dr Rajeev Gupta
Dr Jonathan Lambert (Lymphoma)
Dr Emma Morris (BMT & HSCT)
Dr Ri Liesner
Dr Karl Peggs (BMT & HSCT)
Dr Mary Petrou
(Womens health, Haemoglobinopathy Genetics)
Dr Martin Pule
Dr Neil Rabin (Myeloma)
Dr Farrukh Shah (Joint red cell unit)
Dr Kirsty Thomson (BMT & HSCT)
Dr Sara Trompeter
(Children & young peoples cancer services)
Dr Andres Virchis (Leukaemia)
Professor Anthony Glodstone
(Pathology, Haematological malignancies)
Professor Asim Khwaia (Leukaemia)
Professor David C Linch (Lymphoma)
Professor Stephen Mackinnon
(Bone marrow & Stem cell programme)
Professor John B Porter
(Red cell unit & haemaglobinopathies)
Prof Kwee L Yong (Myeloma & cancer services)
Dr. John Paul Westwood (Haemostasis)
020 7025 7970 or 020 3447 9884
020 3447 9443 or ext 79443
020 3447 8563 or ext 78563
020 3447 9638 or ext 78538
Fax: 020 3447 9911
020 3447 8028 or ext 78028
Fax: 020 3447 9911
020 3447 5239 or ext 75239
020 3447 8483 or ext 78483
020 3447 9443 or ext 79443
020 3447 9712 or ext 79712
020 3447 9884 or ext 79884
020 3447 9712 or ext 79712
Fax: 020 3447 5827
0203 447 9458 or ext 79458
020 3447 8483 or ext 78483
020 3447 8028 or ext 78028
020 3447 9638 or ext 79638
020 3447 9712 or ext 79712
Fax: 020 3447 9864
020 3447 9638 or ext 79638
020 3447 9712 or ext 79712
Fax: 020 3447 9911
020 3447 1528 or ext 71528
020 7679 6554 or ext 79456
020 3447 8483 or ext 78483
Fax: 020 3447 9571
Fax: 020 3447 9571
Fax: 020 3447 9571
Fax: 020 3447 9911
Fax: 020 3447 9911
Email: [email protected]
Email: [email protected]
Email: [email protected]
Email: [email protected]
Email: [email protected]
Email: [email protected]
Email: [email protected]
Email: [email protected]
Email: [email protected]
Email: [email protected]
Email: [email protected]
Email: [email protected]
Email: [email protected]
Email: [email protected]
Email: suzie.knapton’uclh.nhs.uk
Email: [email protected]
Email: [email protected]
Email: [email protected]
Email: [email protected]
Email: [email protected]
020 3447 9712 or ext 79884
Email: [email protected]
020 3447 9638 or ext 79638
020 3447 8028 or ext 78028
Fax: 020 3447 9911
0203 447 6854 or 020 3447 9884 or ext 79884
Email: [email protected]
Email: [email protected]
Email: [email protected]
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Page 6 of 40
Review Date: 10th October 2016
Specialist Registrars
(9.00 – 17.00 Monday – Friday)
Haematology Laboratory SpR
Blood Transfusion Laboratory SpR
Haemostasis Laboratory SpR
On-call Haematology Registrar/SpR (out-of-hours)
Bleep 7000
Bleep 7050
Bleep 7044
Contact via Switchboard
Clinical advice
During routine working hours (Monday – Friday 9.00 – 17.00), please contact the relevant laboratory Specialist Registrar via the bleep system.
A Haematology Registrar/SpR is on call outside routine working hours: contact via the switchboard.
Completion of the request form
Please use the appropriate request form for the tests required and ballpoint (indelible) ink pen to complete the form.

Please provide the patient’s full name, hospital number and date of birth.

Please provide the date and time of sample collection and relevant clinical information to facilitate sample processing and the interpretation of results.
The patient should be aware of the information collected and the purpose for which it is collected.

Please use a separate Blood Transfusion request form for Blood Transfusion requests; Group & Save, Crossmatch, Kleihauer, DAT.

Please ensure that the destination of the report, the name of the requesting/responsible clinician and a contact bleep/telephone number for the
responsible clinician is provided to enable laboratory staff to notify the clinician regarding unsuitable (urgent) samples and to relay and/or discuss urgent
or grossly abnormal results.

Please tick the box next to the test(s) requested; if a required test is not listed, write it clearly into the “Additional Tests” section.

Please tick the appropriate box when the patient is anticoagulated (on warfarin or heparin).

If possible please provide clinical details.
Please ensure that adequate samples are taken for the tests requested and that each sample is labelled correctly. Contact the relevant laboratory for
clarification of sample requirements if required.
Sample Collection (phlebotomy)
The sample collection policy for staff trained in phlebotomy [Haem-PD-BloodSampColl] and guidelines for sample labelling are available on the UCLH Intranet
and can be sent electronically to GP practices.
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Page 7 of 40
Review Date: 10th October 2016
Vacutainer™ Guide
Becton Dickinson
Vacutainer ™
Ordering
Information
Tests / Screens
EDTA
4.5 ml
FBC, ESR, Reticulocyte count, IPF, Blood film (note: ESRs cannot be performed on 7ml EDTA tubes),
Sickle screen.
Glandular Fever testing (note, Glandular Fever testing can be performed on an SST sample),
Lymphocyte subsets (including CD4, CD8 counts), Chronic and Acute Leukaemia Screens, Hereditary
Spherocytosis Dye binding assay,
Plasma Viscosity, PCR in Haemostasis, Haemoglobinopathy Genetics (DNA), Genetic Haemocromatosis
mutations
Flow Cytometry
BM in EDTA for Acute Leukaemia and lymphoma screens.
Kleihauer, DAT
Blood Transfusion
*Group & Save (including antibody screen), *Crossmatch, Kleihauer, DAT
*Sample identification for Group & Screen or Crossmatch MUST be hand written with 4 correct points of ID
Clotting screen / Fibrinogen / D-Dimers. These tubes must be filled exactly to the 4.5ml mark on the side
of the tube: underfilled & overfilled samples are unsuitable for testing.
For Paediatric Clotting Screens, see below.
Citrated Platelet Count (alert Coagulation laboratory staff that the sample is en route: it must not be
centrifuged).
Specialised Haemostasis tests must be authorised by a Haematology/Haemostasis SpR/Consultant
See the Haemostasis section below for the samples required for specialised Haemostasis tests.
Cardiolipin antibodies (Antiphospholipid antibodies), Erythropoietin, Soluble transferring receptor.
 2GPI antibodies
10mls for DNA
EDTA - Blood
Transfusion ONLY
Sodium citrate (citrated
tube)
4.5 ml or 2.7ml
Note : underfilled samples
are unsuitable for testing.
SST
5ml
Lithium Heparin
4.5 ml
Sodium citrate (citrate)
1.0 ml
Haemoglobinopathy screen, G6PD assay, Pyruvate Kinase assay, Red Cell Osmotic Fragility, Globin
Chain Biosynthesis, Oxygen Dissociation curve
Cytogenetic chromosomal analysis and FISH.
Paediatric Clotting Screen, Fibrinogen, D-Dimers (1ml tubes).
Contact the Haemostasis laboratory for other Haemostasis tests for paediatric patients.
These tubes must be filled exactly to the 1ml mark on the side of the tube: underfilled & overfilled samples
are unsuitable for testing.
These tubes are issued from the Haemostasis Laboratory to the Neonatal Unit, Paediatric A&E and
Phlebotomy Outpatient Departments and can be issued to other locations on request: contact x78549 or
x78545. Monday – Friday 09.00 – 17.15
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Page 8 of 40
Review Date: 10th October 2016
Sample Labelling

Please label samples clearly with hospital number, first and last name and date of birth using a ballpoint (permanent/indelible) ink pen. The NHS number
may be used in place of the hospital number for GP and non-UCLH Trust patients.

Samples for a Group and Save (including an antibody screen) and Crossmatching must be hand written with four correct points of ID. Sticky labels are
not permitted. Please include the time and date of collection and the signature of the person collecting the sample.

A minimum of three points of identification must be provided for all other samples.
NOTE: Samples received broken/leaking, inadequately labelled, aged, clotted, haemolysed or otherwise unsuitable for testing will not be processed; an
appropriate comment will be entered onto the computer system. If the requested tests are urgent, laboratory staff will attempt to notify the ward/clinician.
Sample Transport to the Laboratory
Refer to the Pathology Specimen Transport policy on the UCLH Intranet for hospital transport.
Samples from GP practices must be sent via UCLH Trust transport; via the next available collection during the working day. If a significant delay in sample
transportation to the laboratory is anticipated, please discuss with laboratory staff, as sample deterioration may limit the viability of results.
Special Handling Requirements
Please see the ‘Notes’ section in the test tables below, or contact the relevant laboratory for information regarding special handling requirements.
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Page 9 of 40
Review Date: 10th October 2016
Routine Haematology (page 1 of 3)
st
Autolab. 1 Floor, Whitfield St Laboratories, 60 Whitfield St London W1T 4EU

0203 447 8961 or x78961 (Haematology)
Bleep 7060 (out-of-hours)
0203 447 8547 or x78547 (Routine Coagulation)
Please contact the laboratory for reference range(s) if not listed below:
Test
Acanthocytes
Blood Film
Specimen
Type
Reference
ranges
4.5ml EDTA
(can be
performed on
same sample
as FBC)
0-3 %
4.5ml EDTA
(can be
performed on
same sample
as FBC)
Not applicable
Key Factors
affecting tests
Delay in receiving
sample
Sample not kept at
room temperature
Delay in receiving
sample
Sample not kept at
room temperature
Citrated Platelet
Count (platelet
clumping in EDTA)
4.5ml Sodium
Citrate
150-400 x109/l
Clot in sample
ESR
4.5ml EDTA
(can be
performed on
same sample
as FBC)
<40yrs:
1-7 mm/hr (F)
1-5 mm/hr (M)
≥40yrs:
1-20mm/hr
Clot in sample
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Insufficient sample
Delay in receiving
sample
Notes
Out of
hours
service
Send sample ASAP to the
laboratory as prolonged
storage affects cell
morphology
No
service
provided
Send sample ASAP to the
laboratory as prolonged
storage affects cell
morphology.
Service
for
clinically
urgent
requests
Within 24
hours
Service
for
clinically
urgent
requests
Within 24
hours
8 hrs
Service
for
clinically
urgent
requests
Within 24
hours
Urgent
Nonurgent
2 hrs
24 hrs
Please give clinical details
/ reason for request
Please indicate clearly on
the request form that
sample is for a citrated
platelet count. Notify the
Routine Coagulation
Laboratory that the
sample is en-route: do not
centrifuge the sample.
Can be performed on
same sample as FBC if
sample >3mls.
Cannot be performed on a
7ml EDTA tube.
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Time Limit
for
requesting
additional
tests from
time of
venesection
Within 24
hours
Turnaround time
from receipt of
sample
12 hrs
Urgent:
4hrs (not
Nonurgent
24 hrs
out of hours
unless the
laboratory is
contacted)
Page 10 of 40
Review Date: 10th October 2016
Routine Haematology (page 2 of 3)
Test
Specimen Type
Fluid Count
(e.g. Pleural,
Ascitic, Knee fluid)
Fluid to be
taken into EDTA
or 10 or 20ml
sterile container
4.5ml EDTA
Full Blood Count
(FBC)
Reference
ranges
Adult WBC:
9
Key Factors
affecting tests
Notes
Out of
hours
service
Delay in receiving
sample
Please use “Urine/other”
request form-specify type of
fluid
Clot in sample
> 1ml blood required
Sample must be received
within 12 hrs of collection
Service for
clinically
urgent
requests
Service for
clinically
urgent
requests
3-10 x10 l
Neutrophils:
9
2-7.5 x10 /l
Haemoglobin:
115-155g/l (F)
130-170 g/l (M)
MCV: 80-99 fl
Platelets:
Insufficient,
Lipaemic or
Icteric samples
Delayed sample
receipt
Lipaemic/icteric samples can
affect performance of test
and may delay results
Time Limit
for
requesting
additional
tests from
time of
venesection
Within 24
hours
Turnaround time
from receipt of
sample
4 hrs
Sample must
be received
in the
laboratory
within 12
hours of
collection
Urgent:
1 hr
Nonurgent:
8 hrs
Within 7 days
Urgent:
2 hrs
Nonurgent:
24 hrs
9
150-400 x10 /l
Glandular Fever
Screen
4.5ml EDTA
Immature Platelet
Fraction
4.5ml EDTA
Clot in sample
Test can be added on to
existing FBC sample (time
limit: 7 days)
No service
provided
Clot in sample
Test can be added on to
existing FBC sample
Service for
clinically
urgent
requests
Can be performed
on FBC sample
or a 4.5 / 7ml
clotted sample
Can be performed
on FBC sample
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Delayed sample
receipt
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
8 hrs
Page 11 of 40
Review Date: 10th October 2016
Routine Haematology (page 3 of 3)
Test
Reticulocytes
Specimen Type
4.5ml EDTA
Reference
ranges
9
20-100 x 10 /l
Can be performed
on FBC sample
Sickle Screen
4.5ml EDTA
(can be
performed on
same sample as
FBC)
Key Factors
affecting tests
Clot in sample
Delayed sample
receipt
Samples from
children < 6
months old can
give false negative
results
Notes
Out of
hours
service
Time Limit
for
requesting
additional
tests from
time of
venesection
Within 24
hours
Test can be added on to
existing FBC sample (time
limit: 24 hours)
No service
provided
All samples for sickle screens
are referred to Special
Haematology for confirmation
(if not previously confirmed)
Service for Within 7 days
clinically
urgent
requests
Turnaround time
from receipt of
sample
8 hrs
Urgent:
1hr
Anaemia
Clot in sample
Referral Laboratory (Malaria testing)
Parasitology Laboratory
Hospital for Tropical Diseases Building
Capper St
London WC1E 6JA
0845 155 5000 ext 75418
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Page 12 of 40
Review Date: 10th October 2016
Nonurgent:
24 hrs
Blood Transfusion (page 1 of 5)
st
1 Floor, 60 Whitfield St London W1T 4EU
0203 447 8522 / 8523 or x78522 / 78523 (Routine hours)
Bleep 7060 out-of-hours
MAJOR HAEMORRHAGE (see page 4 of this user manual)
 Samples and form MUST carry full and correct patient identification, i.e. handwritten forename, surname, date of birth and hospital number - requests
made without these details will not be accepted.
 Forms must be signed by both the requesting medical officer and the phlebotomist. The phlebotomist must sign and date the sample. Please provide a
bleep number for enquiries.
 Please refer to the Trust Blood Component Transfusion Policy and Procedure available on the UCLH Intranet.
Test
Specimen
Key Factors affecting
Notes
Out of
Time Limit for
Turnaround time from
type
tests
hours
requesting
receipt of sample
service
additional
tests from
time of
venesection
Crossmatch
7ml EDTA
Incorrectly labelled,
Perform a BloodTrack sample
Service for
Contact
Emergency: Group O
(Pink top)
Insufficient, clotted and
enquiry or call the laboratory to
clinically
Laboratory
blood on demand from
haemolysed samples will check whether there is a valid Group urgent
local blood fridges.
be rejected.
& Save available in laboratory before requests
On Demand: From local
sending a new sample.
HemoSafe or HemoNine
blood fridges for eligible
> 2ml required for adults
patients with current,
completed Group & Save
>1ml required for neonates (<4
sample who are suitable
months)
for remote issue.
Urgent/STAT from the
lab: 10 mins plus
transport time for groupspecific blood (only if valid
Group & Save sample
available in laboratory).
Otherwise 45mins for full
crossmatch or 10 mins
uncrossmatched from
receipt of sample in
laboratory plus transport
time.
*If antibodies are present,
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Page 13 of 40
Review Date: 10th October 2016
turnaround time to be
determined on a case by
case basis.
Non-urgent: same day
non-emergency
crossmatch: 2 hours plus
transport time.
*If antibodies are present,
turnaround time to be
determined on a case by
case basis.
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Page 14 of 40
Review Date: 10th October 2016
Blood Transfusion (page 2 of 5)
Test
Direct
Antiglobulin
Test (DAT)
Group & Save
Kleihauer
Specimen
type
Key Factors affecting
tests
4.5 /7ml
EDTA
4.5/7ml
EDTA
4.5ml EDTA
Can be added onto existing Group &
Save or Haematology FBC sample
Insufficient, clotted and
haemolysed samples will
be rejected.
Delay in receiving sample.
Sample not kept at room
temperature before
performing test.
Antenatal:
Anti D/c
quantitation
Anti-K titre
HLA and
disease
association
(e.g. HLAB27)
Notes
7-10ml
EDTA +
sample for
Group &
Save
7-10ml
EDTA
>2ml required for adults
>1ml required for neonates (<4
months)
Service for
clinically
urgent
requests
Service for
clinically
urgent
requests
Time Limit for
requesting
additional
tests from
time of
venesection
Within 5 days
Sample must
be received in
the laboratory
within 12 hours
of collection
Within 5 days
Turnaround time from
receipt of sample
Urgent: 1 hr from receipt
of sample in laboratory
Non-urgent:
24hrs
Urgent: 1 hr from receipt
of sample in laboratory
Non-urgent:
24hrs
24 hrs
Sample should be ideally collected
within 2 hours of delivery or
sensitising event and MUST be
tested within 24 hours (including
Saturdays and Sundays)
Service for
clinically
urgent
requests
Referred to Red Cell
Immunohaematology Department,
North London Blood Transfusion
Centre (NLBTC)
No service
provided
Additional
3 working days
EDTA samples
are required for
test, contact
the laboratory.
No service
provided
Additional
5-7 working days
EDTA samples
are required for
test, contact
the laboratory.
Samples sent on Friday will not be
collected by NLBTC until Monday
Referred to Histocompatibility and
Immunogenetics Department,
NLBTC
Samples sent on Friday will not be
collected by NLBTC until Monday
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Out of
hours
service
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Page 15 of 40
Review Date: 10th October 2016
Blood Transfusion (page 3 of 5)
Test
HLA typing
Specimen
type
Key Factors affecting
tests
7-10ml EDTA
(if white cell count
<2 x109/l send
double volume of
blood)
Referral laboratories:
Red Cell Immunohaematology
North London Blood Transfusion Centre
Charcot Road
London NW9 5BG
0208 957 2831
Notes
Referred to Histocompatibility and
Immunogenetics Department,
NLBTC
Samples sent on Friday will not be
collected by NLBTC until Monday
Out of
hours
service
No service
provided
Time Limit for
requesting
additional
tests from
time of
venesection
Additional
EDTA samples
are required
for test,
contact the
laboratory.
Turnaround time from
receipt of sample
5-7 working days
Histocompatibility and Immunogenetics
North London Blood Transfusion Centre
Charcot Road
London NW9 5BG
0208 957 2812
Requests for routine (scheduled) surgery



Requests must be received in the laboratory by 1900hrs on the day before surgery. A positive antibody screen will delay completion of testing and
provision of compatible units. Patients with known antibodies must be followed up within an agreed time-frame prior to surgery according to their preadmission group and screen report.
Samples received after this time will be prioritised by order of the theatre list. Emergency cases will take priority.
Where Remote Issue is not available, requests for routine surgery should comply with the local Maximum Surgical Blood Ordering Schedule (MSBOS).
Refer to the Trust Blood Transfusion Manual on the intranet.
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Page 16 of 40
Review Date: 10th October 2016
Blood Transfusion (page 4 of 5)
Transport of Routine Blood and Blood Products
Remote issue of red cells has been implemented at UCLH. This allows blood to be issued directly from the Remote Issue blood fridge at the point of care,
rather than from the laboratory for most patients.
A small percentage of patients will still need blood crossmatched by the laboratory but this can also be stored in the remote issue blood fridge and dispensed
when required.
FFP for patients will be sent to remote issue fridges by the laboratory and dispensed in the same way.
Cross matched blood for patients with special requirements and those not eligible for remote issue and other blood products are dispatched as follows:





Non-urgent blood and blood products are dispatched from the laboratory at various times during the day.
Urgent blood and blood products are dispatched from the laboratory by courier (arranged by the laboratory). PLEASE CONTACT THE LABORATORY
(x78523/78522 during routine work hours or Bleep 7060 out of hours) IF BLOOD IS REQUIRED URGENTLY.
*FFP will be returned to the laboratory 24 hours after it has been thawed.
*Unused platelets/FFP/Cryoprecipitate are withdrawn the day after the original date required.
Cryoprecipitate should be used within 4 hours of thawing and any remaining units returned to the lab.
*If the product is no longer required, contact Blood Transfusion immediately. This can reduce blood component wastage if the units can be reissued to a different patient before the expiry time.
Always ring the laboratory before use of EMERGENCY UNCROSSMATCHED BLOOD*
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Page 17 of 40
Review Date: 10th October 2016
Blood Transfusion (page 5 of 5)
Guidelines for Clinical Situations
1. Life threatening haemorrhage (acute massive blood loss) Massive obstetric haemorrhage
Refer to the Major Haemorrhage Algorithm
http://insight/departments/MedicineBoard/Pathology/HaematologyPathology/BloodTransfusion/Documents/Major%20haemorrhage%20algorithm.pdf
For further Information refer to the Trust Major Haemorrhage Protocol
http://insight/guidelines/Clinical%20guidelines/Major%20Haemorrhage%20Protocol%202012.pdf
2. Non-acute bleeding and neonatal surgery
Obtain:
Coagulation screen and FBC
Contact:
Blood Transfusion Registrar
Discuss:
Monday →Friday 09:00-17:00:
All other times:
UCLH Bleep 7050/7000
General Haematology Registrar via UCLH switchboard
Appropriate treatment
(If a high risk procedure, or the patient has a known coagulation abnormality, contact the Haemostasis Registrar prior to
procedure and discuss the patient’s management)
Monday →Friday 09:00 – 17:00
All other times:
Haemostasis Registrar UCH Bleep 7044/7000
General Haematology Registrar via UCH Switchboard
3. Indications for the transfusion of red cells, FFP, cryoprecipitate and platelets
Please refer to the relevant chapter/appendix in the Trust Blood Component Transfusion Procedure Manual
http://insight/guidelines/Clinical%20guidelines/Blood%20Transfusion%20Manual%202012.pdf
4. Patients with Special Transfusion Requirements
For patients who may require CMV negative blood products, Irradiated blood products, Washed red cells and/or HLA matched platelets:
Please refer to the relevant appendix in the Blood Component Transfusion Procedure Manual. Ensure that the laboratory has received a Blood
Transfusion Status Form by emailing BT Status or by faxing to fax number 0203 447 9587 before requesting blood products (this form can be downloaded
from the Blood Transfusion site on the UCLH intranet).
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Page 18 of 40
Review Date: 10th October 2016
Routine Coagulation (page 1 of 2)
st
Autolab 1 Floor, Whitfield St Laboratories, 60 Whitfield St London W1T 4EU






Tel: 0203 447 8547 or x 78547
Citrated samples must be adequately filled (a 9:1 blood:citrate sample ratio is required for all citrated samples). Tests cannot be performed on
underfilled, overfilled, haemolysed or clotted samples.
Paediatric citrated tubes are issued from the Haemostasis Laboratory to the Neonatal Unit, Paediatric A&E and Phlebotomy Outpatient departments and
can be issued to other locations on request: contact x78549/78545 during routine hours.
Include clinical details on the request form, e.g. “patient receiving Warfarin/Heparin therapy” or “post op”; to facilitate appropriate testing and
interpretation of results.
Send the request form and sample to the laboratory as rapidly as possible; notify the laboratory regarding very urgent requests on ext 78547 (during
routine working hours) or Bleep 7060 (out of hours).
Grossly lipaemic samples may require manual testing, potentially delaying results.
Abnormal results that require further laboratory investigation and/or manual testing/confirmation may be delayed.
Test
Coagulation
screen
PT/INR, APTT,
TT, Fibrinogen
Specimen
type
2.7ml or
4.5ml Sodium
Citrate
Key Factors
affecting tests
Heparin &
Warfarin.
Delayed receipt of
sample.
May not be
possible on
grossly lipaemic
samples.
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Note
Reference Range
Out of hours
service
Heparin & warfarin
therapy affects results
and determines
appropriate testing
please indicate that the
patient is on heparin
and/or warfarin on the
request form.
PT: 10-12 secs
APTT: 25-37 secs
TT: 15.5-18.5 secs
Fib: 1.5 – 4.0 g/L
Service for
clinically urgent
requests
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Time Limit
Turnaround time
for
from receipt of
requesting
sample
additional
tests from
time of
venesection
5 hours
Urgent:
Non1hr
urgent:
4 hrs
Page 19 of 40
Review Date: 10th October 2016
Routine Coagulation (page 2 of 2)
Test
D-dimers
(Unfractionated)
Heparin
Monitoring: APTT
ratio
INR
Specimen
type
4.5ml or
2.7ml
Sodium
Citrate
4.5ml or
2.7ml Sodium
Citrate
4.5ml or
2.7ml Sodium
Citrate
Key Factors
affecting tests
Note
This test can be added
for an existing citrated
sample.
Delayed receipt of
sample.
Please indicate Heparin
therapy on the request
form (tick box or
handwrite).
Delayed receipt of
sample.
Please indicate Warfarin
therapy on the request
form (tick box or
handwrite).
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Out of hours
service
Time Limit
Turnaround time
for
from receipt of
requesting
sample
additional
tests from
time of
venesection
5 hours
Urgent:
Non1hr
urgent:
4 hrs
Normal cut off:
0 – 0.55 mg/L FEU
DVT/PE cut off:
<550 ←μg/L FEU
Therapeutic range:
2.0 – 3.0
Service for
clinically urgent
requests
Service for
clinically urgent
requests
5 hours
Urgent:
1 hour
Nonurgent:
4 hours
Overall range:
1.5 – 4.5
An individual
patient’s target
range is a clinical
decision: contact
the Anticoagulation
Clinic (ext
75222/x79139, 9-5
Mon-Fri) for target
ranges or seek
advice from the
Haemostasis SpR
on bleep 7044
(during routine
hours) or via the
switchboard (outof-hours).
Service for
clinically urgent
requests
5 hours
Urgent:
1 hour
Non
urgent:
4 hours
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Page 20 of 40
Review Date: 10th October 2016
Haemostasis (page 1 of 6)
nd
Hours: 09.00 – 17.15 Monday - Friday
2 Floor, 60 Whitfield St London W1T 4EU
Tel: 0203 447 8545 / 8549, x78545 / 78549
All Haemostasis tests must be requested/authorised by the Haemostasis SpR (Bleep 7044), Haematology SpR (Bleep 7050) or Haematology Consultant, with
the exception of the following:
 Cardiolipin Antibody testing
 Plasma Viscosity measurement
 Thrombophilia Screens from the Hyper Acute Stroke Unit (HASU) and the National Hospital for Neurology and Neurosurgery
 Antiphospholipid Antibody Screens from Rheumatology Outpatients (Professor Isenberg’s Clinic).
Please discuss requests with Haemostasis laboratory staff if necessary, to ensure adequate samples are collected.
 Citrated samples must be adequately filled (a 9:1 blood:citrate sample ratio is required for all citrated samples).
 Tests cannot be performed on underfilled, overfilled, haemolysed or clotted citrated samples.
 Paediatric citrated tubes are issued from the Haemostasis Laboratory to the Neonatal Unit, Paediatric A&E and Phlebotomy Outpatient departments and
can be issued to other locations on request: contact x78549/78545 during routine hours.
 Include clinical details on the request form to facilitate appropriate testing and interpretation of results.
 Send the request form and sample to the laboratory as rapidly as possible; plasma must be processed within 5 hours of venepuncture.
 For very urgent requests, notify the laboratory staff on ext 78549 / x78545 (during routine working hours) or Bleep 7060 (out of hours).
Test
Anti-Xa assay
For monitoring low
molecular weight
heparin –(LMWH)
therapy.
Specimen type
1 x 4.5ml Sodium
Citrate sample
Key Factors
affecting
tests
Delayed
receipt of
sample
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Notes
Reference
Range
Out of hours
service
Time Limit for
requesting
additional
tests from
time of
venesection
Turnaround
time from
receipt of
sample
Samples should be collected
4 hours post an injection of
Low Molecular Weight
Heparin.
Therapeutic
range: 0.5 –
1.0 IU/ml.
By
agreement
with the
Haematology
Consultant
on call and
subject to
staff
availability.
Contact the
Laboratory
Urgent:
12 hrs
This test requires
authorisation by clinical
Haematology staff.
Individual
patient’s
target range
is a clinical
decision.
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Page 21 of 40
Review Date: 10th October 2016
Non-urgent:
5 working
days
Haemostasis (page 2 of 6)
Test
Antiphospholipid
Antibody Screen
/ Lupus Screen
incorporates
Lupus
Anticoagulant
testing and
Cardiolipin
Antibody testing
Specimen type
2 x 4.5 ml Sodium
Citrate samples
and
1 x 5ml SST
sample
Key Factors
affecting tests
Notes
Reference
Range
Out of hours
service
Time Limit for
requesting
additional tests
from time of
venesection
Warfarin,
Heparin and
Rivaroxaban
therapy.
Lupus
Anticoagulant
testing is
inappropriate
when patients are
receiving heparin
therapy.
Contact the
laboratory
regarding Lupus
Anticoagulant
testing.
No out of hours
service
Contact the
Laboratory
IgG:  12
GPLU/ml
IgM:  12
MPLU/ml
Delayed receipt
of sample (Lupus
Anticoagulant
test).
Refer to AntiCardiolipin
Antibody testing.
Cardiolipin
Antibodies
(ACLA)
1 x 5ml SST
sample
This test does not
require
authorisation.
2 Glycoprotein
1 Antibodies
(B2GPI)
1 x 5ml SST
sample
Lupus
Anticoagulant
test
3 x 4.5ml Sodium
Citrated samples
A single SST
IgG: < 10 U/ml
sample is
IgM: < 6 U/ml
sufficient for
ACLA & B2GPI
Antibody testing.
Lupus
Anticoagulant test
is inappropriate
for patients on
heparin therapy.
Dilute Russell’s
Viper Venom
Time test
Warfarin, Heparin
and Rivaroxaban
therapy.
Delayed receipt
of sample
or Taipan Venom
Time test (on
warfarin).
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Turnaround time
from receipt of
sample (in
working days
for non urgent
requests)
3 working days
No out of hours
service
3 working days
No out of hours
service
Contact the
Laboratory
3 working days
No out of hours
service
Contact the
laboratory
3 working days
An SST sample is
required if
Cardiolipin
Antibody testing
is also requested.
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Page 22 of 40
Review Date: 10th October 2016
Haemostasis (page 3 of 6)
Test
Specimen type
Key Factors
affecting tests
Notes
Reference
Range
Out of hours
service
Time Limit for
requesting
additional
tests from
time of
venesection
Turnaround
time from
receipt of
sample (in
working
days for non
urgent
requests)
Thrombophilia
screen
4 x 4.5ml Sodium
Citrated samples
+
1 x 5ml SST sample
+
1 x 4.5ml EDTA
Delayed receipt
of sample.
These tests require
authorisation unless the
patient is a
Haematology, NHNN or
HASU patient: for all
other patients, please
contact the Haemostasis
or Haematology SpR
(bleep 7044 or 7050) for
authorisation before
taking samples.
Contact the
laboratory
Antithrombin,
Protein C
and Protein S
only, by
agreement
with the
Haematology
Consultant
on call and
subject to
staff
availability.
Contact the
Laboratory
3 working
days
Antithrombin activity,
Protein C activity,
Free Protein S,
Lupus Anticoagulant,
Cardiolipin Antibodies,
Factor V Leiden,
Prothrombin Gene
Mutation,
Also Factor VII and X
assays when patients
are on warfarin.
Plasma must be
processed within
5 hours of
venesection.
Warfarin and
Heparin
Acute illness may
affect results of
some tests
AT Ag, PS
activity, 10
working days
Samples for Factor V
Leiden, MTHFR and
Prothrombin Gene
Mutation are sent from
Haemostasis, UCLH to
TDL Genetics
Antithrombin Antigen,
Protein C Antigen and
Protein S activity may
be performed when
abnormal results are
obtained in above
assays
MTHFR mutation.
These tests can be
requested individually.
1 x 4.5ml EDTA
A single EDTA
sample is adequate
for FVL, PGM and
MTHFR mutations
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
This test requires
authorisation unless
requested by a
Haematologist.
PC Ag, 20
working days
No out of
hours service
Contact the
Laboratory
Referred to TDL.
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Page 23 of 40
Review Date: 10th October 2016
3 working
days
Haemostasis (page 4 of 6)
Test
Bleeding screen
1) von Willebrand
Factor Antigen
2) von Willebrand
Factor Activity
3)
Factor VIII assay
4) Platelet
Aggregation
Platelet Aggregation
studies
Specimen type
Key Factors
affecting
tests
7 x 4.5ml Sodium
Citrated samples
Heparin affects
FVIII result.
Recommendation:
also send an EDTA
sample for a FBC
and citrated
sample for
Coagulation
screen.
Delayed receipt of
sample.
7 x 4.5ml Sodium
Citrated samples
Aspirin affects
platelet
aggregation:
testing is not
recommended.
Delayed receipt of
sample.Samples
not kept at room
temperature.
Recommendation:
also send an EDTA
sample for a FBC)
Notes
Reference
Range
Out of hours
service
Time Limit for
requesting
additional
tests from
time of
venesection
Turnaround
time from
receipt of
sample (in
working
days for non
urgent
requests)
FVIII:
0.50 – 2.00
IU/ml
Contact the
Laboratory
3 working
days
VWFAct:
0.50 – 1.87
IU/ml
Factor VIII
only, by
agreement
with the
Haematology
Consultant
on call and
subject to
staff
availability.
Contact the
laboratory
No out of
hours service
Tests must be
performed
within 5
hours of
venepuncture.
1 working
day
VWFAg:
0.50 – 1.60
IU/ml
Samples must be kept at
room temperature and
received by the lab within
1.5 hours of phlebotomy
and before 1200 hrs.
Significant
thrombocytopaenia
: aggregation
studies only
possible with an
adequate platelet
count
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Page 24 of 40
Review Date: 10th October 2016
Haemostasis (page 5 of 6)
Test
PFA-100
(Platelet function
analysis)
Specimen type
Key Factors
affecting
tests
Notes
1 x 4.5ml Sodium
Citrated sample
Aspirin affects
results.
Recommendation:
also send an EDTA
sample for a FBC)
Delayed
receipt of
sample.
The sample must be kept at
room temperature & tested
within 5 hours.
Sample not
kept at room
temperature.
Reference
Range
Out of hours
service
Time Limit for
requesting
additional
tests from
time of
venesection
Turnaround
time from
receipt of
sample
Contact the
laboratory
No out of
hours service
Contact the
Laboratory
1 working
day
Contact the
laboratory
Factor VIII +
IX only, by
agreement
with the
Haematology
Consultant
on call and
subject to
staff
availability.
No out of
hours service
Contact the
Laboratory
3 working
days
Contact the
laboratory.
20 working
days
As for factor
assays
Contact the
laboratory.
20 working
days
Platelet Aggregation
Studies must be requested
separately if required.
Significant
thrombocytope
nia, anaemia,
leucopaenia.
4.5ml Sodium
Citrated samples –
Heparin and
Warfarin.
contact the
laboratory for the
number of samples
required (dependent
on tests requested).
Acute illness
(contact the
laboratory).
Fibrinogen Antigen
4.5 ml Sodium
Citrate sample
Delayed
sample receipt
Bethesda Inhibitor
Assay
2 x 4.5 ml Sodium
Citrate sample
Delayed
sample receipt
Factor Assays
FVIII, FIX, FXI, FXII
FII, FV, FVII, FX
FXIII
Required factor assays
must be specified on the
request form.
Delayed
receipt of
sample
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
1.5 – 4.0 g/L
Only appropriate where
factor levels extremely low.
Contact the
laboratory
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
For FXIII, 5
working days
Page 25 of 40
Review Date: 10th October 2016
Haemostasis (page 6 of 6)
Test
Heparin induced
thrombocytopaenia
(HIT) screen
Heparin:PF4 antibody
assay
Plasma viscosity
Specimen type
Key Factors
affecting
tests
Notes
Referenc
e Range
1 x 4.5ml
Sodium
Citrated
sample
(preferred)
Urgent test:
send the
sample to the
lab.
Immediately.
This test MUST be authorised by a
Haematology Consultant (i.e. not a
SpR): contact via the Haematology
SpR
(bleep 7044/7050) or
switchboard out of hours.
Contact
the
laboratory
1 x 4.5ml
EDTA sample
Variations in
temperature
Samples must be kept at room
temperature.
1.4 - 1.75
mPa
Delayed
receipt of
sample
Samples that have been refrigerated
are unsuitable for testing.
Samples should not be warmed, but
warming does not make them
unsuitable for testing
Out of
hours
service
Time Limit
for
requesting
additional
tests from
time of
venesection
Turnaround
time from
receipt of
sample (in
working
days for
non urgent
requests)
By agreement
with the
Haematology
Consultant
and subject
to staff
availability.
Contact the Urgent:
Laboratory 1 working
day
By
agreement
with the
Haematology
Consultant
on call and
subject to
staff
availability.
7 days
Non-urgent:
5 working
days
2 working
days
Referral Laboratories:
For Rivaroxaban assay, ADAMTS 13, PF1.2
and TAT (Thrombin antithrombin complex)
Haemostasis Research Unit,
Department of Haematology
University College London Hospitals
1st Floor, 51 Chenies Mews
London WC1E 6HX
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
For VWF Multimers and Nucleotides
Haemophilia Centre and Thrombosis Unit,
Ground Floor
Royal Free Hospital
Pond Street
London NW3 2QG
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
For Factor V Leiden, Prothrombin Gene
Mutation and MTHFR Mutation and FXIII
The Doctor’s Laboratory,
60, Whitfield St.
London W1T 4EU
Page 26 of 40
Review Date: 10th October 2016
Special Haematology (page 1 of 3)
2nd Floor, 60 Whitfield St London W1T 4EU


Hours: 09.00 -17.15 Monday – Friday
0203 447 8533 or x78533
Haemoglobinopathy & Haemolytic Anaemia investigations
Reference ranges are provided on the report and on the IT system.
Test
Haemoglobinopathy
screen
Specimen type
Key Factors
affecting tests
Notes
4.5ml EDTA/Lithium Age of sample
heparin + sample for
FBC
Can be
sample
performed
Out of
hours
service
on
FBC No service
currently
Time Limit for
requesting
additional
tests from
time of
venesection
Within 7 days
%Hb S levels
4.5ml EDTA/Lithium
heparin
Can be
sample
performed
on
FBC No service
currently
Within 7 days
Hb Electrophoresis
(CA, CE or acid gel
reflex tests)
G6PD levels
4.5ml EDTA/Lithium
heparin
Can be
sample
performed
on
FBC No service
currently
Within 7 days
4.5ml EDTA or
Lithium heparin
Sample not stored
at 2-6 °C
Pyruvate Kinase
levels
4.5ml Lithium
heparin
Sample not stored
at 2-6 °C
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Recent blood transfusion can
invalidate the result. Please
indicate if patient has been
transfused recently.
Recent blood transfusion can
invalidate the result. Please
indicate if patient has been
transfused recently.
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
No service
currently
Within 7 days
No service
currently
Within 48
hours
Turnaround
time from
receipt of
sample (in
working days)
Urgent:
2 hours if no
further testing
required
Non urgent:
72 hours if no
further testing
required
Urgent:
2 hours
Non urgent:
24 – 48 hours
5 days
Urgent:
2 – 4 hours
Non urgent:
48 hours
Urgent:
24 hours
Non urgent:
72 hours
Page 27 of 40
Review Date: 10th October 2016
Special Haematology (page 2 of 3)
Test
Specimen type
Key Factors
affecting tests
Notes
Haemoglobin H stain
4.5ml EDTA or Lithium
heparin
Age of sample
Samples greater than 48 hours
old are unsuitable for this test.
Heinz Body
Preparation
4.5ml EDTA or Lithium
heparin
Age of sample
Hereditary
Spherocytosis
Dye Binding Assay
4.5ml EDTA
Age of sample
Red Cell Osmotic
Fragility
Red Cell Oxygen
Dissociation
Methaemoglobin
screen
Globin Chain
Biosynthesis
4.5ml Lithium heparin
Please arrange with laboratory
4.5ml Lithium heparin
Please arrange with laboratory
4.5ml EDTA
N/A
3 x 4.5ml Lithium
heparin (10ml blood
required)
Please arrange with laboratory
Out of
hours
service
Time Limit for
requesting
additional tests
from time of
venesection
Turnaround
time from
receipt of
sample (in
working days)
No service
provided
24 hours
48 hours
Samples greater than 48 hours
old are unsuitable for this test.
No service
provided
48 hours
72 hours
Please arrange with laboratory
No service
provided
Within 8 hours
48 hours
No service
provided
No service
provided
No service
provided
No service
provided
Within 8 hours
48 hours
Within 8 hours
48 hours
Samples greater than 24 hours
old are unsuitable for this test.
48
Within 8 hours
Urgent
7 working days
Non urgent
14 days
Haemoglobinopathy
DNA and
Haemochromatosis
Erythropoietin
2 - 4 x 4.5ml EDTA
(10 - 20ml blood
required)
7ml clotted sample (no
anticoagulant)
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Referred to Haemoglobinopathy
Genetics, UCLH
No service
provided
No service
provided
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Additional
samples are
required for test.
Additional
samples are
required for test.
Page 28 of 40
Review Date: 10th October 2016
3-20 working
days
14 days
Special Haematology (page 3 of 3)
Test
Specimen type
Key Factors
affecting tests
Notes
Out of hours
service
Time Limit for
requesting
additional tests
from time of
venesection
Turnaround
time from
receipt of
sample
Soluble Transferrin
Receptor assay
7ml clotted sample
Via Haematology Consultant.
Referred to Kings College
Hospital
No service
provided
Additional
samples are
required for test.
4-8 weeks
Transcobalamin
7 ml clotted sample
Referred to Royal Free Hospital. No service
Sample must be separated within provided
2 hours. Referred to Royal Free
Hospital
Additional
samples are
required for test.
4-6 weeks
Liver Dry Iron
Estimation
Biopsy sample in
Formalin
Referred to Royal Free Hospital.
Additional
samples are
required for test.
2-6 weeks
No service
provided
Referral Laboratories:
Haemoglobinopathy Genetics
Haemoglobinopathy Genetics Centre
Molecular Genetics Laboratory
307 Euston Road
London NW1 3AD
0203 447 9458
Department of Clinical
Chemistry
Kings College Hospital
Denmark Hill
London SE5 9RF
0207 737 4000 ext 3501
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Special Haematology
Royal Free Hospital
Pond Street London NW3 2QG
0207 794 0500 ext 33413
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
For Paroxysmal Nocturnal
Haemoglobinuria (PNH):
Haematological Malignancy
Diagnostic Service
Level 3 Bexley Wing
St James University Hospital
Beckett Street
LEEDS LS9 7TF
Page 29 of 40
Review Date: 10th October 2016
Haemoglobinopathy Genetics (Page 1 of 1)
Haemoglobinopathy Genetics,
Molecular Genetics Laboratory,
307 Euston Road, London NW1 3AD
Hours 09.00-17.00
Tel: 0203 447 9458
For further information on the molecular testing provided by this laboratory please see separate document: Haemoglobinopathy Genetics User Handbook
(HAEM-PD-HGUserHbook)
,
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Page 30 of 40
Review Date: 10th October 2016
Flow Cytometry (HIV and Immunophenotyping) (page 1 of 5)
2nd Floor, 60 Whitfield St, London W1T 4EU

Hours: 09.00 – 17.15 Monday - Friday
Tel: 0203 447 9603 or x 79603
Please see report or telephone laboratory for reference ranges
Test
Acute Leukaemia
Screen. This
includes
Myeloperoxidase
stain, Esterase stain
Specimen Type
Bone Marrow in EDTA
(can be performed on
peripheral blood if
bone marrow is not
available)
No minimum volume.
Analysis can also be
carried out on CSF,
pleural and ascites
fluid.
Chronic Leukaemia
Screen
Key
Factors
affecting
tests
Notes
Out of hours service
Time Limit for
requesting
additional tests
from time of
venesection
Turnaround time
from receipt of
sample (in
working days)
Heavily clotted
sample
Please discuss with
Haematology SpR (Bleep
7000/7001) before sending.
The service at weekends
and Bank Holidays is for
new acute leukaemia
screens only and must be
backed by Consultant
Haematologist. Contact
Laboratory on-call bleep
7060. Clinical staff must
be available for
interpretation of results as
this will not be provided by
laboratory staff.
Within 24 hours
Urgent: If lab is
pre notified of
urgent samples
results can be
authorised in 8
hours.(reported by
SpR and
Consultant)
No service provided
Within 24 hours
Pleural,
Ascites and
CSF samples
not being
spun onto
slides within
12 hours of
being taken.
Please let Flow Cytometry
laboratory know to expect
sample.
Transport samples at room
temperature.
Peripheral Blood in
EDTA
Heavily clotted
sample
Can be performed on
BM, CSF, pleural fluid
and Ascites fluid.
Pleural, Ascites
and CSF
samples not
being spun onto
slides within 12
hours of being
taken
No minimum volume.
Please send samples
directly to SIHMDS
Specimen Reception
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Please send samples
directly to SIHMDS
Specimen Reception
Non-urgent:
48hrs (reported by
SpR and
Consultant)
Please let Flow Cytometry
laboratory know to expect
the samples.
Non-urgent: 48hrs
(reported by SpR
and Consultant)
Transport samples at room
temperature.
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Urgent: If lab is pre
notified of urgent
samples results can
be authorised in 8
hours (reported by
SpR and Consultant)
Page 31 of 40
Review Date: 10th October 2016
Flow Cytometry (HIV and Immunophenotyping) (page 2 of 5)
Test
Lymphoma Screen
Specimen Type
Bone Marrow or
Peripheral Blood
(Can be performed on
existing FBC sample)
Key
Factors
affecting
tests
Heavily
clotted
sample
No minimum volume.
Notes
Please send samples directly to
SIHMDS Specimen Reception.
Out of hours
service
No service provided
Time Limit
for
requesting
additional
tests from
time of
venesection
Within 24
hours
Please let flow cytometry
laboratory know to expect
sample.
Transport samples at room
temperature.
Bone Marrow Aspirate
8 x bone marrow
smears for morphology
(all slides MUST be
labeled with hospital
number, surname and
date of sample. (Please
note, DOB is not
required, date of sample
is crucial)
2 EDTA for
Immunophenotyping
and/or Molecular Testing
Slides not
dried
thoroughly.
Slides not
labeled
correctly
BM slides and aspirate must be
delivered to Specialist Integrated
Haematological Malignancy
Diagnostic Service (SIHMDS) via
courier. Courier pick up from
Cancer Centre and phlebotomy
already in place.
Heavily
clotted
sample.
Transport at room temperature
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Urgent: : If lab is
pre notified of
urgent samples
results can be
authorised in 8
hours (reported by
SpR and
Consultant)
Non-urgent:
48hrs.
BM received out of
hours which need to
be processed out of
hours must be
communicated to
haematology out of
hours bleep (7060).
They will be stained
and ready for
reporting by
registrar.
Within 24
hours
1 Heparin for CGN
and/or Molecular Testing
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Turnaround
time from
receipt of
sample (in
working days)
Page 32 of 40
Review Date: 10th October 2016
Urgent ( i.e.
Acute
Leukaemia):
Same day for
reporting by
Haem
SpR/consultant
Non-urgent:
48 hours
(reported by SpR
and Consultant)
Flow Cytometry (HIV and Immunophenotyping) (page 3 of 5)
Test
Specimen Type
Lymphocyte subsets for 1 x 4.5ml EDTA
HIV monitoring and for
(Adult)
haematology patients.
1 x 0.5ml (Paediatric)
Key
Factors
affecting
tests
Clotted
sample
4.5ml EDTA
(Can be performed on
existing FBC sample)
Please discuss all HIV negative
requests with Haem SpR (Bleep
7000/7001)
Out of hours
service
Time Limit
for
requesting
additional
tests from
time of
venesection
Turnaround
time from
receipt of
sample (in
working days)
No service provided
Within 24
hours
24hrs (reported
by registered
Laboratory staff)
No service provided
Within 24
hours
24hrs (reported
by registered
Laboratory staff)
Samples should be <24hours old.
(Can be performed on
existing FBC sample)
CD19
(for patients receiving
anti-CD20 Rituximab)
Notes
Samples received after 1600hrs
will not be processed till the next
working day
Clotted
sample
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Transport at room temperature
Transport at room temperature.
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Page 33 of 40
Review Date: 10th October 2016
Flow Cytometry (HIV and Immunophenotyping) (page 4 of 5)
Test
Cerebrospinal Fluid
(CSF)
Specimen Type
Key
Factors
affecting
tests
CSF in 10/20ml sterile
container
Sample not
delivered as
soon as
they have
been taken.
No minimum volume
Notes
Between 9.00am-4.30pm
samples need to be delivered by
hand to SIHMDS specimen
reception. After 4.30pm they
need to be hand delivered to
main Haematology Lab on 1st
floor at 60 Whitfield St.
If white cells are seen upon
microscopic examination of
cytospin slides, they are referred
to Haem SpR for verification and
may be immunophenotyped if
indicated.
Out of hours
service
Time Limit
for
requesting
additional
tests from
time of
venesection
Turnaround
time from
receipt of
sample (in
working days)
All CSF requests
received out of
hours will be partially
processed but not
reported. If they
need to be looked at
out of hours the oncall lab staff need to
be contacted (7060)
Within 24
hours
24hrs (reported
by registered
laboratory staff
and Consultant)
If sample needs
immunophenotyp
ing then TAT will
be increased.
No service provided
Within 24
hours
48 hours
(reported by SpR
and Consultant)
Transport at room temperature
Iron stain
8 x Bone Marrow
Slides
If there are
no granules
present on
slides.
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Slides must be labelled in pencil
with hospital number, surname
and date of sample. (DOB is not
required)
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Page 34 of 40
Review Date: 10th October 2016
Flow Cytometry (HIV and Immunophenotyping) (page 5 of 5)
Test
Specimen Type
Key
Factors
affecting
tests
Notes
Out of hours
service
Time Limit
for
requesting
additional
tests from
time of
venesection
Turnaround
time from
receipt of
sample (in
working days)
Urinary Haemosiderin
10mls urine
None
Haemosiderin slides are
prepared by lab staff and need
24 hours to dry before being
stained.
No service provided
Within 24
hours
72 hours
(reported by
SpR)
Pleural or Ascites fluid
1ml fluid in sterile
container
Sample not
delivered as
soon as
they have
been taken.
Between 9.00am-4.30pm
samples need to be delivered by
hand to SIHMDS specimen
reception. After 4.30pm they
need to be hand delivered to
st
main Haematology Lab on 1
floor at 60 Whitfield St.
No service provided
Within 24
hours
24hrs (reported
by registered
laboratory staff
and Consultant)
If sample needs
immunophenoty
ping then TAT
will be
increased.
If white cells are seen upon
microscopic examination of
cytospin slides, they are referred
to Haem SpR for verification and
may be immunophenotyped if
indicated.
Transport at room temperature
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Page 35 of 40
Review Date: 10th October 2016
Specialist Integrated Haematological Malignancy Diagnostic (SIHMDS) (page 1 of 5)
nd
2 Floor, 60 Whitfield St London W1T 4EU
Hours: 09.00 – 17.00 Monday - Friday
Tel: 0203 447 8518 or x 78518
All immunophenotyping, cytogenetics, molecular and histology samples will be received at SIHMDS and referred to destination laboratories.
Test
Cytogenetic (chromosomal analysis)
-Testing for suspected haematological
disease disorders
(at presentation, post therapy or bone
marrow transplant).
Aplastics/ general anaemia
Acute Myeloid Leukaemia –
M1,M2,M3,M4,M4Eo,M5
A.L.L
Burkitt’s Lymphoma
CLL
CML
Diffuse Large B cell Lymphoma
ET
Follicular Lymphoma
Mantle cell lymphoma
MDS (CMML/RA/RARS/RAEB)
Myelofibrosis
Multiple Myeloma
MPD (ET/Polycythaemia)
NHL
Pancytopenia
Plasma cell Leukaemia
PRV
Thrombocytopenia/Neutropenia
Specimen
type
Bone Marrow
Peripheral
Blood:
5-10ml
Samples
should be in
Lithium
Heparin or
Cytopots
(RPMI 1640 +
Heparin
medium).
Except for
CD138+
isolated
plasma cells
for FISH on
multiple
myeloma
cases.
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Key Factors
affecting tests
Poor sample
quality (clotted
or more than a
day old)
Sample
transported and
or stored above
or below room
temperature
Sample
Contaminated
by bacteria
Sample taken
into wrong
transport
medium
Notes
Out of
hours
service
Time Limit
for
requesting
additional
tests from
time of
venesection
Sent to: Great Ormond Street
Hospital, Paediatric
Malignancy Unit (PMU)
No
service
provided
N/A
Samples must be received
before 2pm (Monday-Friday)
for samples to get to PMU on
the same day.
The samples may be left
overnight at room temperature
and delivered to laboratory
immediately the following
morning.
Authorised by: Debbie Mann
Urgent
referrals:
10 working
days
Routine
requests:
15 working
days
Analysis will be carried as
appropriate or in consultation
with the referring Consultant
or Registrar.
Haematology and Blood Transfusion User Handbook
Turnaround
time from
receipt of
sample (in
working
days)
Page 36 of 40
Review Date: 10th October 2016
Specialist Integrated Haematological Malignancy Diagnostic (SIHMDS) (page 2 of 5)
Test
Cytogenetic FISH (Fluorescent
Insitu Hybridisation) Analysis
-Testing for suspected
haematological disease disorders
(at presentation, post therapy or
bone marrow transplant).
Aplastic anaemia
AML-M1,M2,M3,M4,M4Eo,M5
A.L.L
Burkitt’s Lymphoma
CLL
CML
Diffuse Large B cell Lymphoma
ET
Follicular Lymphoma
Hypereosinophilic Syndrome
Mantle cell lymphoma
MDS
Multiple Myeloma
MPD (ET/Polycythaemia)
NHL
Plasma cell Leukaemia
PRV
Specimen type
As above
Bone Marrow or
Peripheral Blood
in EDTA for FISH
only.
CD138+ isolated
plasma cells for
FISH on multiple
myeloma cases is
preferable
however bone
marrow samples
may also be
adequate.
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Key Factors
affecting tests
Poor sample
quality (clotted
or more than a
day old)
Sample
transported
and or stored
above or below
room
temperature
Sample
Contaminated
by bacteria
Sample taken
into wrong
transport
medium
Notes
Sent to: Great Ormond Street
Hospital, Paediatric Malignancy
Unit (PMU)
Out of
hours
service
Time Limit
for
requesting
additional
tests from
time of
venesection
Turnaround
time from
receipt of
sample (in
working
days)
No
service
provided
N/A
Rapid FISH:
3 working
days
Samples must be received before
2pm (Monday-Friday) for samples
to get to PMU on the same day.
Urgent
referrals:
10 working
days
Analysis will be carried as
appropriate or in consultation with
the referring Consultant or
Registrar.
Routine
requests:
15 working
days
The samples may be left
overnight at room temperature
and delivered to laboratory
immediately the following
morning.
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Page 37 of 40
Review Date: 10th October 2016
Specialist Integrated Haematological Malignancy Diagnostic (SIHMDS) (page 3 of 5)
Test
JAK-2 mutation analysis
Specimen type
Peripheral
Blood/Bone
Marrow in EDTA
Key Factors
affecting
tests
Notes
Sent to: Great Ormond Street
Hospital, Paediatric Malignancy
Unit (PMU)
Out of
hours
service
Time Limit
for
requesting
additional
tests from
time of
venesection
Turnaround
time from
receipt of
sample (in
working
days)
No
service
provided
N/A
10 working
days
No
service
provided
N/A
10 working
days
Samples must be received before
2pm (Monday-Friday) for samples
to get to PMU on the same day.
Analysis will be carried as
appropriate or in consultation with
the referring Consultant or
Registrar.
PCR


Peripheral Blood
in 10-20ml EDTA
BCR/ABL
Ph+ve ALL
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
The samples may be left overnight
at room temperature and delivered
to laboratory immediately the
following morning.
Sent to: Imperial College Molecular
Pathology Laboratory
Hammersmith Hospital
Samples will be sent the following
day if received after 2pm. Samples
need to arrive before midday on
Fridays and prior to bank holidays
which span a weekend
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Page 38 of 40
Review Date: 10th October 2016
Specialist Integrated Haematological Malignancy Diagnostic (SIHMDS) (page 4 of 5)
Test
Notes
Out of
hours
service
Time Limit
for
requesting
additional
tests from
time of
venesection
Peripheral
Blood/Bone
Marrow in EDTA
Sent to Wessex Regional Genetics
Laboratory, Salisbury District Hospital
No
service
provided
N/A
10 working
days
Chimerism Studies
T-cell receptor
rearrangement
Peripheral
Blood/Bone
Marrow in EDTA
Sent to: Department of Haematology,
Royal Free Hospital
No
service
provided
N/A
10 working
days
Sent to HMDS,
St James University Hospital
No
service
provided
N/A
MRD
IgVH
Peripheral
Blood/Bone
Marrow in EDTA
10 working
days
No
service
provided
N/A
5 working
day
PDGFR receptor


CLL


PNH testing
Specimen type
PB sample
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Key
Factors
affecting
tests
Samples will be sent the following day
if received after 3pm. Samples need
to arrive before midday on Fridays
and prior to bank holidays which span
a weekend
Samples will be sent the following day
if received after 2pm. Samples need
to arrive before midday on Fridays
and prior to bank holidays which span
a weekend
Samples will be sent the following day
if received after 3pm. Samples need
to arrive before midday on Fridays
and prior to bank holidays which span
a weekend
Sample
needs to
be under
24 hours
All PNH screens are referred to
Leeds Royal Infirmary. SIHMDS
needs to be phoned the day before
the sample arrives to the lab in order
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Page 39 of 40
Review Date: 10th October 2016
Turnaround
time from
receipt of
sample (in
working
days)
old
to book a courier to Leeds. The
sample needs to be in the lab at
12.00pm at the latest in order to be
sent the same night. Samples which
are not expected by the lab and/or
arrive after 12.00pm will not get to
Leeds in time for processing.
Samples must not be taken on
Friday. Thursday 12.00pm is the very
latest for any given week.
Specialist Integrated Haematological Malignancy Diagnostic (SIHMDS) (page 5 of 5)
Referral laboratories:
Paediatric Malignancy Unit
Level 2 Camelia Botnar Laboratories
Great Ormond Street Hospital
Great Ormond Street
London WC1N 3JH
Haematological Malignancy Diagnostic Service
(HMDS)
St James Institute of Oncology
Level 3 Bexley Wing
St James University Hospital
Breckett Street
Leeds. LS9 7TF
HAEM-PD-UserHbook V9
Authors: Karen Orfinada (Senior Biomedical Scientist)
Sally McDonald (Senior Biomedical Scientist), Yurdum Mohammad (BMS)
Imperial College Molecular Pathology
Laboratory
G-block 2nd floor, D2.04
Hammersmith Hospital
Du Cane Road
London W12 0HS
Wessex Regional Genetics Laboratory
Salisbury District Hospital
Odstock Road
Salisbury
Wiltshire SP2 8BJ
Haematology and Blood Transfusion User Handbook
Authorised by: Debbie Mann
Department of Haematology
Royal Free Hospital
Pond Street
Hampstead
London NW32QG
Page 40 of 40
Review Date: 10th October 2016