Figure S4
A)
mpn347a
HP?
mpn348a (DOC Toxin)
log2 (Coverage)
ncMPN322
10
5
0
415200
415400
415600
415800
Genome Position (bp)
B)
ClustalW Aligment DOC protein of M. pneumoniae FH
and MPN348a of M.pneumoniae M129
DOC M.pneumoniae FH
MPN348a
MREINVYYLSNDSKSDKDDIDYLIEELLVEGSRFKNSLVTFLEKEKDCQS
VREINVYYLSNDSKSDKDDIDYLIEELLVEGSRFKNSLVTFLEKEKDCQS
:*************************************************
CICLFKSKLSSSFEIRVKFNELNLDASDEKTKEFLRYIVSKSINNAKFLS
CICLFKSKLSSSF-IRVKFNELNLDASDEKTKEFLRYIVSKSINNAKFLS
************* ************************************
SEDFDDKKEDEILSIIENTFNSYCYKSDLLSPSLYCKEGRLNIIDLINDI
SEDFDDKKEDEILSIIENTFNSYCYKSDLLSPSLYCKEGRLNIIDLINDI
**************************************************
FCKVLTFHKLSNGNKRLATALLVNFLYTFGYYFKWTIPLKKEEYFKYAHM
FCKVLTFHKLSNGNKRLATALLVNFLYTFGYYFKWTIPLKKEEYFKYAHM
**************************************************
IKNFVVSFEEHKRENRDEFLAKQTKKFIMDNILLALNFWETKLN 244
IKNFVVSFEEHKRENRDEFLAKQTKKFIMDNILLALNFWETKLN 243
********************************************
DOC M.pneumoniae FH
MPN348a
DOC M.pneumoniae FH
MPN348a
DOC M.pneumoniae FH
MPN348a
DOC M.pneumoniae FH
MPN348a
50
50
100
99
150
149
200
199
Multiple sequence alignment of fic motif in Doc family members
MPN
Pyrolobus fumarii
Pseudomonas stutzeri
2EVO
3EQX
VopS
C)
DIFCKVLTFHKLSNGNKRLATALLVNFLYTF
ALFYEAITRHPLTDGNKRFAVVLLRAFLKAN
HLLYFVIKNHPFSDGNKRSAAFLFVDFLYRN
AKYVEMNIAHPFLEGNGRS-TRIWLDLVLKK
XAHYQFEAIHPFIDGNGRT-GR-VLNILYLI
HLFAGVIGYHGFTDGNGRM-GR-MLYAIAEL
Conservation of genomic context mpn155a
nusA
mpn155a
infB
ClustalW alignment between
mpn155a and YLxR S. pneumoniae
CLUSTAL 2.1 multiple sequence alignment
mpn155a
YlxR
MQS----WRQCLVTRKSFAKPLLLRLVK-LEHQLRVDLAQTLPGRGYYLSVAALKLERKN 55
MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANGRGAYIKLDNAEALEAK 60
*::
*:.:*:.: : * ***:** * *: :* : . *** *:.:
: . :
mpn155a
YlxR
FKALLQKRLKVSCSDAELDHIIACLKE--RESDVKA- 89
KKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE 97
* :::: :.:. .:: *.:** :.. :. ::