Microalgae
culturing
and
maintenance
BioPlant
workshop
/
advanced
course
9–13
September
2013
Department
of
Biology
–
University
of
Aveiro
ANNOUNCEMENT
Microalgae
are
a
highly
diverse
assemblage
of
phylogenetic
lineages
that
have
acquired
the
photosynthetic
machinery
invented
by
cyanobacteria.
They
are
the
focus
of
intense
research,
not
least
because
they
hold
the
key
to
understanding
major
evolutionary
steps
in
eukaryotes.
The
establishment
of
cultures
of
microalgae
is
an
important
basis
for
studies
of
these
organisms
and
offers
the
opportunity
for
the
exploration
of
a
huge
diversity
of
biocompounds
and
of
the
biomass
they
produce.
This
post‐graduate
advanced
workshop,
organized
in
the
framework
of
the
BioPlant
MAP
joint
doctoral
programme,
introduces
the
fundamental
steps
for
establishing
cultures
of
(mainly)
photosynthetic
protists,
from
cell
or
organism
isolation
out
of
field
samples
to
culture
maintenance
in
the
laboratory.
It
addresses
isolation,
culture
media
both
for
marine
and
freshwater
protists,
maintenance
conditions,
and
the
management
of
a
culture
collection.
Some
aspects
of
culture
studies,
particularly
large
cell
number
screening
by
Flow
Cytometry,
will
be
included.
Culture
cryopreservation
techniques
will
be
explained
and
demonstrated.
The
course
will
combine
lectures
with
extensive
practical
sessions
mainly
covering
preparation
of
culture
media
and
isolation
methods
of
cells,
colonies
or
filamentous
microalgae
of
marine,
estuarine
and
freshwater
origin.
Methods
involving
the
direct
handling
of
single
cells
or
organisms
with
the
help
of
stereo‐
or
inverted
microscopes
will
be
given
particular
attention.
Flow
cytometry
techniques
will
be
demonstrated
with
pre‐established
cultures.
Cryopreservation
of
algal
cultures
will
be
discussed
and
demonstrated.
Contents
•
Introduction
to
the
groups
of
microscopic
protists,
especially
those
with
photosynthetic
capacity
•
Culture
media
for
freshwater,
marine
and
estuarine
species
•
Isolation
methods
•
Growth
conditions
and
culture
maintenance
Microalgae
culturing
and
maintenance
BioPlant
workshop
/
advanced
course
9–13
September
2013
Department
of
Biology
–
University
of
Aveiro
•
Management
and
commercial
viability
of
a
culture
collection
•
Flow
cytometry
as
a
tool
for
culture
studies
•
Cryopreservation
theory
and
practice
Language,
Participants
and
Registration
Fees
The
course
will
be
conducted
in
English.
Course
attendance
is
free
for
BioPlant
students
and
for
students
enrolled
in
doctoral
programmes
offered
by
Dept.
Biology,
UA.
Registration
fee
for
other
participants
is
100€.
The
maximum
number
of
participants
is
15.
Credits
Participants
will
be
awarded
3
ECTS
credits,
of
which
2
correspond
to
the
attendance
of
the
five
days
of
activities
and
1
to
the
delivery
of
an
individual
report,
in
the
format
of
a
scientific
article,
describing
the
procedure
for
establishing
a
uni‐algal
culture
of
a
species
at
the
student’s
choice.
Course
coordinators
António
José
Calado,
Dept.
Biology
and
GeoBioTec
Research
Unit,
University
of
Aveiro,
Portugal
Sandra
Carla
Craveiro,
Dept.
Biology
and
GeoBioTec
Research
Unit,
University
of
Aveiro,
Portugal
Invited
lecturers
Gert
Hansen,
head
(curator)
of
the
Scandinavian
Culture
Collection
of
Algae
and
Protozoa
(SCCAP)
at
the
University
of
Copenhagen,
Denmark
–
main
lecturer
Lília
Santos,
professor
in
the
Dept.
of
Life
Sciences,
University
of
Coimbra,
head
of
the
Culture
Collection
of
Algae
at
Coimbra
(ACOI),
Portugal
–
cryopreservation
With
the
collaboration
of
Helena
C.C.
Oliveira,
Dept.
Biology
and
CESAM
Research
Unit,
University
of
Aveiro,
Portugal
–
flow
cytometry
Maria
Conceição
Santos,
Dept.
Biology
and
CESAM
Research
Unit,
University
of
Aveiro,
Portugal
–
flow
cytometry
Registration:
Please
send
filled
in
registration
form
(see
next
page)
to
[email protected]
Registration
deadline:
4
September
2013.
Microalgae
culturing
and
maintenance
BioPlant
workshop
/
advanced
course
9–13
September
2013
Department
of
Biology
–
University
of
Aveiro
Registration
form
Fill
in
and
send
to:
[email protected]
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/
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/
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