Technical Note
TD026EN Ver1.2
Cellufine PB
--
100
Cellufine PB can be used
at high flow rates.
Bed height [ % ]
Linear flow rate [ cm/hr ] - ○ -
1800
The flow/pressure curve for a Cellufine PB column
confirms operating flow rates above 1600 cm/h can
be obtained. The compressibility of Cellufine PB is
approximately 7%.
1600
90
1400
1200
80
1000
800
70
600
400
60
200
0
0.000
0.020
0.040 0.060 0.080
Pressure [ MPa ]
50
0.120
0.100
Relative capacity,Conalbumin [ % ]
Fig1. Pressure-flow rate curve for Cellufine PB.
Column: I.D. 9 cm, bed height: 38 cm
Mobile phase: water
Media particle size: 125-210 µm.
100
Cellufine PB is alkali stable
80
When stored in 0.5M NaOH for 600 hours at 30 ℃, the
adsorption capacity of Conalbumin remained stable.
When stored 1.0M NaOH for 600 hours at 30 ℃,
adsorption of Conalbumin only decreased slightly.
60
40
0.5M NaOH at 30ºC
20
1.0M NaOH　at 30ºC
0
0
100
200
300
400
time ( hours )
500
600
Fig2. Stability of Cellufine PB under alkaline
conditions at 30 ºC.
Adsorption capacity of virgin media = 100 %
Reletive Capacity,Conalbumon [%]
100
Cellufine PB is acid stable.
When stored in 0.2M HCl for 3 months at 40 ℃, the
adsorption capacity of Conalbumin remained stable.
80
60
40
20
0
0
1
2
3
time [ month ]
Fig3. Stability of Cellufine PB under acidic
conditions (0.2M HCl) at 40 ºC.
Data: an average of 3 lots.
TD026EN Ver1.2 (April 1, 2011)
1
Technical Note
TD026EN Ver1.2
Relative capacity,Ovalbumin [ % ]
100
Cellufine PB performance
remains constant over at
least 200 operating cycles.
90
80
70
60
50
0
50
100
cycle
150
200
Fig 4. Stability of Cellufine PB at CIP.
Flow rate: 1.5 CV/h at 25 ℃
Equilibrium buffer: 0.01M sodium phosphate,
pH 7.0 + 0.15 M NaCl
Regeneration solution: 0.5 M NaOH
CIP: 1) equilibrium buffer 3 CV
2) regeneration solution 3 CV
Relative capacity ,conalbumin [%]
100
Cellufine PB can be
Autoclaved repeatedly.
80
60
Autoclaving is recommended in pure water .
40
20
0
0
2
4
6
Autoclave cycle
8
10
Fig 5. Change of adsorption capacity in Cellufine PB
during autoclaving cycle.
Conditions: pure water ,20 minutes at 121℃
Data: an average of 3 lots
Cellufine PB conforms to USP28, Plastic Class V.
2-1, Otemachi 2-chome, Chiyoda-ku
Tokyo 100-8105 Japan
[email protected]
TD026EN Ver1.2 (April 1, 2011)
2