Kenneth Vincent Mills
College of the Holy Cross
Department of Chemistry
1 College Street
Worcester, MA 01610
Phone: 1-508-793-3380
FAX: 1-508-793-3530
E-mail: [email protected]
Employment
2001-present College of the Holy Cross, Department of Chemistry, Worcester, MA
•Professor, 2014•Department Chair, 2010 - 2014
•Associate Professor, 2007-2014
•Assistant Professor, 2001-2007
Education
1996-2001
Harvard University, Cambridge, MA
•Department of Chemistry and Chemical Biology
•A.M. 1998, Ph.D. 2001, Advisor: Prof. Henry Paulus
1992-1996
Loyola College in Maryland, Baltimore, MD
•B.S., summa cum laude, 1996, Chemistry major, philosophy minor
Research Interests
Protein splicing, mechanistic enzymology, protein chemistry, guided-inquiry
undergraduate lab development
Research Experience
2001-present College of the Holy Cross, Department of Chemistry, Worcester, MA
•Direct undergraduate researchers in the study of the mechanism of
hedgehog autoprocessing and protein splicing.
1996-2001
Harvard University, Department of Chemistry and Chemical Biology,
Cambridge, MA
•Studied mechanism of protein splicing and developed an in vitro transsplicing protein ligation system using inteins from RecA protein of
Mycobacterium tuberculosis and DnaE protein of Synechocystis species
PCC6803.
1995
Summer Research Institute for Applied Science, Lawrence Livermore
National Laboratory, Livermore, CA
•Studied peptide models of prion protein folding.
1
1991-1996
Medical Research Institute for Chemical Defense, Aberdeen Proving
Grounds, Edgewood Area, MD
•Studied and designed active site mutants of human butyrylcholinesterase
that demonstrated hydrolysis of organophosphate inhibitors.
Teaching Experience
2001-present College of the Holy Cross, Department of Chemistry
•Montserrat-Drug Design, Action and Abuse (Natural World cluster, Fall
2012 and 2013)
•Montserrat-Biotechnology and Ethics (Natural World cluster, Spring
2013 and 2014)
•Atoms and Molecules, Chemistry 101/181 Lecture and Lab (Fall 2001,
2002, 2004, 2005, 2006, 2008)
•Introduction to Equilibrium and Reactivity/Equilibrium and Reactivity,
Chemistry 231 Lecture and Lab (Spring 2002-2007, 2009-12)
•Biochemistry, Chemistry 301, Fall 2008, 2010-13
•Bioorganic Chemistry, Chemistry 310 (Spring 2003, 2006)
•Advanced Biochemistry, Chemistry 363 (Spring 2004)
•Biochemistry II Laboratory, Biology 304 (Spring 2002)
2000-2001
Harvard University, Division of Continuing Education
•Head teaching fellow for General Chemistry course.
1997-2000
Harvard University, Division of Continuing Education
•Teaching fellow for General Chemistry
1996-1997
Harvard University, Department of Chemistry and Chemical Biology
•Teaching fellow for Organic Chemistry
External Grants
2015-2018
National Science Foundation, RUI Grant, $748,268
RUI:TheCatalysisofProteinSplicingandtheStructureandStability
ofThermophilicInteins:AnIntegratedResearchandEducation
Program
(http://news.holycross.edu/blog/2015/10/15/with-1-5m-in-nsf-fundingholy-cross-professor-makes-student-research-opportunities-a-priority/)
2013-2016
National Science Foundation, RUI Grant, $567,115
RUI: Intein Structure and Catalysis of Protein Splicing and Hedgehog
Autoprocessing: An Integrated Research and Education Program
2012
National Science Foundation, REU supplement to RUI grant, $6,500
2010-2013
National Science Foundation, RUI Grant, $465,000
2
RUI: Catalysis and Coordination of the Steps of Protein Splicing by Noncanonical Inteins: An Integrated Research and Education Program
2009-2016
Henry Dreyfus Teacher Scholar Award, $60,000
2010
National Institutes of Health, Subaward, $25,000
Subaward to collaborator Chunyu Wang’s NIH ARRA supplement
(Rensselaer Polytechnic Institute)
2005-2010
National Science Foundation, CAREER grant, $794,482
“CAREER: Alternative Mechanisms of HINT domain autoprocessing: An
integrated undergraduate research and education program”
2009
American Society for Biochemistry and Molecular Biology,
Undergraduate Outreach Award, $250
2003-2005
National Science Foundation, Major Research Instrumentation, $255,502
“Acquisition of Protein Purification and Analysis Equipment to Enhance
Biochemical Research and Education at the College of the Holy Cross”
2003-2005
Research Corporation, Cottrell College Science Award, $58,028
“The Mechanism and Molecular Evolution of Hedgehog Proteins and
Related Auto-Processing Domains”
2002-2004
Petroleum Research Fund (American Chemical Society), $35,000
Type GB, “The Mechanism of Protein Splicing and the Molecular
Evolution of Inteins.”
1997-2001
Howard Hughes Medical Institute Predoctoral Fellowship
1995-1996
Barry M. Goldwater Fellowship
Internal Grants
2013
Hewlett Mellon Presidential Discretionary Fund
Summer Workshop on Emerging Challenges in STEM Education
2012
Hewlett Mellon Presidential Discretionary Fund, Chemistry
Chemistry Department Review: Reflection and Response
2009
Hewlett Mellon Presidential Discretionary Fund, Biochemistry
Concentration Assessment: Refinement and Implementation
2008
Hewlett Mellon Presidential Discretionary Fund, Biochemistry
Concentration Assessment: Mission, Goals and Student Learning
Outcomes
3
2003
Research and Publication Award
2002
Charles and Rosanna Batchelor (Ford) Foundation Summer Fellowship
(declined to accept external funding)
Awards/Fellowships
2015
2013
2009
2009
2002
1997-2001
1996, 1997
1996
1994-1996
1994-1996
1996
1996
1995
1992-1996
1992-1996
1992-1993
Cottrell Scholar, Research Corporation
Mary Louise Marfuggi Award – Outstanding Scholarly Accomplishments
Henry Dreyfus Teacher Scholar Award, Dreyfus Foundation
Undergraduate Outreach Award, American Society for Biochemistry and
Molecular Biology
Charles and Rosanna Batchelor (Ford) Foundation Faculty Fellowship
Howard Hughes Medical Institute Predoctoral Fellowship
National Science Foundation Fellowship honorable mention
American Institute of Chemists, D.C. chapter, chemistry award
Barry M. Goldwater Scholar
Datatel Scholar
Award for highest overall GPA, Loyola College in Maryland
Chemistry Medal, Loyola College in Maryland
Phi Beta Kappa, Alpha Sigma Nu (Jesuit Honor Society)
Dean's List, Loyola College in Maryland, all semesters
Marion Burk Knott Scholarship, Maryland Distinguished Scholar
National Merit Scholar, Byrd Scholar
Publications (Undergraduate co-authors indicated by asterisk)
Holy Cross publications:
30. Mills, K.V. (2015) Biochemistry in an undergraduate writing-intensive first-year
program: Seminar courses in drugs and bioethics. Biochemistry and Molecular Biology
Education 43, 263-272.
29. Kulevich, S.E., Herrick, R.S., and Mills, K.V. (2014) A Discovery Chemistry
Experiment on Buffers. J. Chem. Educ. 91, 1207-1211.
28. Mills, K.V., Johnson, M.A., and Perler, F.B. (2014) Protein splicing: How inteins
escape from precursor proteins. Journal of Biological Chemistry, 289, 14498-14505.
27. Topilina, N.I. and Mills, K.V. (2014) Recent Advances in in vivo applications of
intein-mediated protein splicing. Mobile DNA 5, 5.
4
26. *Nicastri, M.C., *Xega, K., Li, L., Xie, J., Wang, C., Linhardt, R.J., Reitter, J.N., and
Mills, K.V. (2013) Internal Disulfide Bond Acts as a Switch for Intein Activity.
Biochemistry 52, 5920-5927.
25. Mills, K.V. (2013) Self-Splicing Proteins. In: Handbook of Proteolytic Enzymes,
Third Edition, Vol. 1., ed. N. Rawlings and G. Salvesen, 315-321.
24. Chen, W., Li, L., Du, Z., Liu, J., Reitter, J.N., Mills, K.V., Linhardt, R.J. and Wang,
C. (2012) Intramolecular Disulfide Bond between Catalytic Cysteines in an Intein
Precursor. J. Am. Chem. Soc.,134, 2500-2503.
23. Du, Z., Liu, J., Albracht, C.D., Hsu, A., Chen, W., *Marieni, M.D., *Colelli, K.M.,
*Williams, J.E., Reitter, J.N., Mills, K.V. and Wang, C. (2011) Structural and Mutational
Studies of a Hyperthermophilic Intein from DNA Polymerase II of Pyrococcus abyssi. J.
Biol. Chem. 286, 38638-38648. (Selected for Nov. 4, 2011 cover.)
22. Liu, J., Du, Z., Albracht, C.D., Naidu, R.O., Mills, K.V. and Wang, C. (2011) 1H, 13C,
and 15N NMR assignments of the Pyrococcus abyssi DNA polymerase II intein. Biomol.
NMR Assign. 5, 233-235.
21. Reitter, J.N. and Mills, K.V. (2011) Canonical protein splicing of a class one intein
that has a class three non-canonical sequence motif. J. Bacteriol. 194, 994-997.
20. *O’Brien, K.M., *Schufreider, A.K., *McGill, M.A., *O’Brien, K.M., Reitter, J.N.
and Mills, K.V. (2010) Mechanism of protein splicing of the Pyrococcus abyssi lon
protease intein. Biochem. Biophys. Res. Commun. 403, 457-461.
19. *Kerrigan, A.M., *Powers, T.L., *Dorval, D.M., Reitter, J.N., and Mills, K.V. (2009)
Protein splicing of the three Pyrococcus abyssi ribonucleotide reductase inteins. Biochem.
Biophys. Res. Commun. 387, 153-157.
18. Mills, K.V., Herrick, R.S., Ditzler, M.A., Nestor, L.P., Shafer, H., and Guilmette,
L.W. (2008) An Introduction to Electrochemistry: A Two-week Discovery Chemistry
Experiment. J. Chem. Educ. 85, 1116-1119.
17. Herrick, R.S., Mills, K.V., and Nestor, L.P. (2008) Data Pooling in a Chemical
Kinetics Experiment: The Aquation of a Series of Cobalt (III) Complexes. J. Chem.
Educ. 85, 1120-1122.
16. Mills, K.V. and Guilmette, L.W. (2007) Thermochemical Analysis of Neutralization
Reactions: An Introductory Discovery Experiment. J. Chem. Educ. 84, 326-328.
15. Bellin, R.M. and Mills, K.V. (2007) The Holy Cross Biochemistry Concentration:
An Integrated Four-Year Program to Develop Undergraduate Research Scholars. In
Developing and Sustaining a Research-Supportive Curriculum, ed K.K. Karukstis and
T.E. Elgren, Council on Undergraduate Research.
5
14. Mills, K.V., Connor, K.R., *Dorval, D.M., and *Lewandowski, K.T. (2006) Protein
Purification via Temperature-Dependent, Intein-Mediated Cleavage from an Immobilized
Metal Affinity Resin. Anal. Biochem. 356, 86-93.
13. Mills, K.V., *Dorval, D.M., *Lewandowski, K.T. (2005) Kinetic analysis of the
individual steps of protein splicing for the Pyrococcus abyssi PolII intein. J. Biol. Chem
280, 2714-2720.
12. Mills, K.V., *Gareau, J.R., and *Garcia, A.M. (2005) Low-cost modification to the
Copacabana method for spreading transformation mixtures. BioTechniques 39, 188.
11. Mills, K.V., Perler, F.B. (2005) The Mechanism of Intein-Based Protein Splicing:
Variations on a Theme. Protein & Peptide Letters, 12, 751-755.
10. Mills, K.V. (2005) Protein Splicing. In Encyclopedia of Molecular Cell Biology and
Molecular Medicine, Second Edition, ed, RA Meyers, 241-265, Weinheim: Wiley-VCH.
9. Mills, K.V., Paulus, H. (2005) Biochemical Mechanisms of Intein-Mediated Protein
Splicing. In Nucleic Acids and Molecular Biology 16 (Homing Endonucleases and
Inteins), ed. M. Belfort, 231-255, Berlin: Springer Verlag.
8. Mills, K.V., *Manning, J.S., *Garcia, A.M., *Wuerdeman, L.A. (2004) Protein
splicing of a Pyrococcus abyssi intein with a C-terminal glutamine. J. Biol. Chem. 279,
20685-20691.
7. Mills, K.V. (2004) Self-Splicing Proteins. In: Handbook of Proteolytic Enzymes,
Second Edition, Vol. 2., ed. AJ Barrett, 2136-2140, London: Academic Press, Elsevier
Science.
6. Mills, KV. (2003) Post-Translational Control. In: Genetics, Vol. 3., ed. R Robinson,
178-182, New York: Macmillan Reference USA.
Pre-Holy Cross Publications:
5. Mills, K.V., Paulus, H. (2001) Reversible Inhibition of Protein Splicing by Zinc Ion.
J. Biol. Chem. 276, 10832-10838.
4. Lew, B.M., Mills, K.V., Paulus, H. (1999) Characteristics of Protein Splicing in trans
Mediated by a Semisynthetic Split Intein. Biopolymers (Peptide Science) 51, 355-362.
3. Lew, B.M., Mills, K.V., Paulus, H. (1998) Protein Splicing in Vitro with a
Semisynthetic Two-Component Minimal Intein. J. Biol. Chem.273, 15887-15890.
6
2. Mills, K.V., Lew, B.M., Jiang, Sq, Paulus, H. (1998) Protein splicing in trans by
purified N- and C- terminal fragments of the Mycobacterium tuberculosis RecA intein.
Proceedings of the National Academy of Sciences, USA 95, 3543-3548.
1. Broomfield, C.A., Mills, K.V., Meier, B.M., Lockridge, O.A., and Millard, C.B.
(1998) Reaction of human butyrylcholinesterase (BchE) H117 enzymes with carbamates.
In: Structure and Function of Cholinesterases and Related Proteins, 223-226.
Abstracts (Recent, first author presenter)
Jaramillo, M., Williams, J., Reitter, J. and Mills, K. (2015) Structure and Activity of
Inteins from Pyrococcus abyssi and Pyrococcus horikoshii. FASEB J. 29, 722.4.
Yakely, A., Bonano, A., Jaramillo, M., Williams, J., Reitter, J., and Mills, K. (2015)
Relating Intein Flexibility to the Temperature Dependence of Activity. FASEB J. 29,
722.3.
Siegart, N., Colelli, K., Reitter, J. and Mills, K. (2015) Protein Splicing of a TemperatureDependent Intein from an Extreme Thermophile. FASEB J. 29, 573.12.
(Best Undergraduate Poster – Proteins and Enzymes:
www.asbmb.org/News.aspx?id=63127)
Giaccone, Z., Reitter, J., Steeves, A., Kulik, H. and Mills, K. (2015) Peptide Bond
Cleavage through Asparagine Cyclization. FASEB J. 29, 722.9.
Koulopoulos, M., Giaccone, Z., Reitter, J., and Mills, K. (2015) A Biophysical Analaysis
of Asp Cyclization Coupled to Peptide Bond Cleavage. FASEB J. 29, 722.2
Mills, K. (2015) Integrating Biochemistry into a First-year Undergraduate RhetoricIntensive Seminar Program. FASEB J. 29, 559.1.
Mills, K., Reitter, J., Nicastri, M., Colleli, K., and Williams, J. (2015) Protein splicing:
Regulation by Temperature and Oxidation State of Non-Canonical Inteins, Biophysical
Journal 108 (2-Sup.1), 30a 144-Plat.
*Jaramillo, M.V., *Nicastri, M.C., Reitter, J.N. and Mills, K.V. (2014) Protein splicing of
an intein from the extreme halophile Halobacterium salinarum. FASEB J. 28, 768.9.
*Haley, D.S., *Siegart, N.M., Reitter, J.N. and Mills, K.V. (2014) Structural analysis of
human carbonic anhydrase II and a thermophilic intein. FASEB J. 28, 768.3.
*Cahn, S.A.,Reitter, J.N. and Mills, K.V. (2014) Directed evolution toward inteins with
alternative protein splicing mechanisms. FASEB J. 28, 566.2.
7
*Giaccone, Z.T., *Urbanski, L.M., *Siegart, N.M., Reitter, J.N. and Mills, K.V. (2014)
Peptide bond cleavage through cyclization of asparagine. FASEB J. 28, 768.4.
*Siegart, N.M., *Colelli, K.M., *Williams, J.E., Reitter, J.N. and Mills, K.V. (2014)
Protein splicing of a temperature-dependent intein from an extreme thermophile. FASEB
J. 28, 768.10.
Mills, K.V., Reitter, J.N., *Nicastri, M.C., *Williams, J.E., and *Colelli, K.M (2014) The
stops and starts of protein splicing: regulation and mechanism of non-canonical inteins.
FASEB J. 28, 584.1.
*Siegart, N.M., *Urbanski, L.M., *Karanja, K., Reitter, J.N. and Mills, K.V. (2013)
Protein Splicing of Non-canonical Inteins from Cyanobacteria Protein Science 22-Sup1,
234.
Mills, Kenneth V., Reitter, J.N., *Nicastri, M.C., *Williams, J.E., *Colelli, K.M., and
*Marieni, M.D. (2013) The Structure and Regulation of Non-Canonical Inteins. Protein
Science 22-Sup1, 93.
*Williams, J.E., Reitter, J.N. and Mills, K.V. (2013) Structural analysis of an intein from
an extreme thermophile. FASEB J. 27, 1005.1. (Honorable Mention – Best
Undergraduate Poster)
*Colelli, K.M., *Pusztay, J.M., Reitter, J.N. and Mills, K.V. (2013) The role of an
extended beta-sheet in stabilizing the structure of a thermophilic intein. FASEB J. 27,
1005.2. (Honorable Mention – Best Undergraduate Poster)
*Nicastri, M.C., *Xega, K., Reitter, J.N., and Mills, K.V. (2013) Conditional protein
splicing via disulfide bond formation. FASEB J. 27, 789.3.
*Siegart, N.M., *Urbanski, L.M., *Karanja, K., *Colelli, K.M., Reitter, J.N. and Mills,
K.V. (2013) Protein splicing of inteins from Synechococcus sp. PCC7002 and
Trichodesmium erythraeum. FASEB J., 27, 789.7.
*Cahn, S.A., *Savidis, G., *Drago, M.J., Reitter, J.N. and Mills, K.V. (2013) Expression
and auto-processing of hedgehog-like proteins from Brugia malayi and Cryptosporidium.
FASEB J. 27, 789.4.
*Pusztay, J.M., *Drago, M.J., *Powers, T.L., *Schufreider, A.K., Connor, K.R., Reitter,
J.N., and Mills, K.V. (2013) Splicing of a non-canonical class three intein from
Clostridium thermocellum. FASEB J. 27, 789.5.
*Urbanski, L.M., *Chin, S.L., Reitter, J.N. and Mills, K.V. (2013) Peptide bond cleavage
adjacent to asparagine or glutamine. FASEB J. 27, 789.6.
8
Mills, K.V., Reitter, J.N., Connor, K.R., *Nicastri, M.C., *Williams, J.E., *Colelli, K.M.,
and *Marieni, M.D. (2013) The structure, regulation and activity of non-canonical
inteins. FASEB J. 27, 998.2.
*Savidis, G., *Drago, M.J., Reitter, J.N., Mills, K.V. (2012) Expression and autoprocessing of hedgehog-like domains from Brugia malayi. FASEB J. 26, 753.2.
*Colelli, K.M., Reitter, J.N. and Mills, K.V. (2012) Protein splicing of a temperaturedependent intein from an extreme thermophile. FASEB J. 26, 756.3. (Honorable
Mention – Best Undergraduate Poster)
*Williams, J.E., Reitter, J.N. and Mills, K.V. (2012) Protein splicing facilitated by highly
similar inteins from two extreme thermophiles. FASEB J. 26, 756.4.
*Karanja, K.K., *Urbanski, L.M., Reitter, J.N. and Mills, K.V. (2012) Protein splicing of
inteins from Synechococcus sp. PCC 7002 and Pyrococcus abyssi. FASEB J. 26, 756.5.
*Urbanski, L.M., *Chin, S.L., Reitter, J.N. and Mills, K.V. (2012) Side-chain cyclization
of Gln or Asn coupled to peptide bond cleavage in an intein and a model peptide. FASEB
J. 26, 756.6.
*Chin, S.L., *Nadelson, A.C., *Urbanski, L.M., Reitter, J.N. and Mills, K.V. (2012)
Intein-mediated peptide bond cleavage adjacent to asparagine or glutamine. FASEB J. 26,
963.6.
Mills, K.V., Reitter, J.N., Connor, K.R., *Nicastri, M.C., *Urbanski, L.M., *Williams,
J.E., *Colelli, K.M., *Savidis, G. and *Marieni, M.D. (2012) Non-canonical inteins:
Protein splicing by alternate mechanisms. FASEB J. 26, 959.2.
*Nicastri, M.C., *Xega, K., Reitter, J.N. and Mills, K.V. (2012) Conditional protein
splicing of inteins with a non-canonical C-terminal glutamine. FASEB J. 26, 959.1.
Mills, K., Reitter, J., *Dorval, D., *Lewandowski, K., *Marieni, M., *Duffee, L.,
*Donahue, C. (2011) Non-canonical inteins: Alternate mechanisms for protein splicing.
Protein Science 20-Sup 1, 180.
*Xega, K., Reitter, J.N. and Mills, K.V. (2011) Post-translational autoprocessing by an
Oryza sativa hedgehog-like domain. FASEB J. 25, 754.16.
*O’Brien, K.M., *Schufreider, A.K., *McGill, M.A., *O’Brien, K.M., Reitter, J.N. and
Mills, K.V. (2011) Post-translational autoprocessing of a Pyrococcus abyssi intein and a
Cryptosporidium hedgehog-like domain. FASEB J. 25, 754.10.
*Marieni, M.D., *Duffee, L.R., *York, D.J., *Dorval, D.M., Reitter, J.N. and Mills, K.V.
(2011) The influence of conserved catalytic residues on the mechanism of protein
splicing of the Pyrococcus abyssi PolII intein. FASEB J. 25, 520.10.
9
*Donahue, C.A., *York, D.J., *Dorval, D.M., Reitter, J.N. and Mills, K.V. (2011)
Determining the activation barrier and pH-dependence of each step of protein splicing.
FASEB J. 25, 520.6.
*Chin, S.L., *Nadelson, A.C., *York, D.J., Reitter, J.N. and Mills, K.V. (2011) Utilizing
a protein splicing side reaction to generate a fragment for expressed protein ligation.
FASEB J. 25, 520.3.
Mills, K.V., *Powers, T.L., *Drago, M.J., Connor, K.R., and Reitter, J.N. (2011) Noncanonical inteins: Alternate mechanisms for protein splicing. FASEB J. 25, 520.11.
*Duffee, L.R., *York, D.J., *Dorval, D.M., Reitter, J.N. and Mills, K.V. (2010) A kinetic
analysis of each step of protein splicing of the Pyrococcus abyssi PolII intein. FASEB J.
24, 463.5.
Mills, K.V., Reitter, J.N., *Duffee, L.R., *York, D.J., *Dorval, D.M., and
*Lewandowski, K.T. (2010) Non-canonical inteins: alternate mechanisms for protein
splicing. FASEB J. 24, 520.11.
*Nadelson, A.C., *York, D.J., *Dorval, D.M., *Lewandowski, K.T., Connor, K.R.,
Reitter, J.N. and Mills, K.V. (2010) Manipulation of protein splicing side reactions to
facilitate protein purification and expressed protein ligation. FASEB J. 24, 463.13.
*York, D.J., *Duffee, L.R., *Dorval, D.M., Reitter, J.N. and Mills, K.V. (2010)
Estimating the activation barrier to each step of protein splicing for the non-canonical P.
abyssi PolII intein. FASEB J. 24, 463.18. (Honorable Mention – Best Undergraduate
Poster)
*Duffee, L.R., *Nadelson, A.C., *York, D.J., *Dorval, D.M., Reitter, J.N. and Mills,
K.V. (2009) Influence of conserved residues on individual steps of protein splicing of the
non-canonical Pyrococcus abyssi PolII intein. Protein Science 18-Sup1, 86. Selected for
an oral presentation.
*Nadelson, A.C., *York, D.J., *Lewandowski, K.T., *Dorval, D.M., Connor, K.R.,
Reitter, J.N. and Mills, K.V. (2009) Development of protein purification strategies using
the Pyrococcus abyssi PolII intein. Protein Science 18-Sup1, 86.
Mills, K.V., Reitter, J.N., Connor, K.R., *Kerrigan, A.M., *Dorval, D.M., *Drago, M.J.,
*Lewandowski, K.T. and *Powers, T.L. (2009) Non-canonical inteins: Alternate
mechanisms of protein splicing. Protein Science 18-Sup1, 171.
*Schufreider, A.K., *McGill, M.M., *O’Brien, K.M., Reitter, J.N, and Mills, K.V. (2009)
Protein Splicing of the Pyrococcus abyssi lon protease intein. FASEB J. 22, 502.2.
(Honorable Mention – Best Undergraduate Poster)
10
*Kerrigan, A.M., *Powers, T.L., *Dorval, D.M., Reitter, J.N. and Mills, K.V. (2009) The
influence of extein residues on the protein splicing of three Pyroccocus abyssi inteins.
FASEB J. 22, 502.3. (Honorable Mention – Best Undergraduate Poster)
*York, D.J., Nadelson, A.C., Reitter, J.N., and Mills, K.V. (2009) Alternate protein
splicing mechanisms: A directed evolution approach. FASEB J. 22, 502.2.
Reitter, J.N., *Schufreider, A.K., and Mills, K.V. (2009) Protein Splicing by a Noncanonical Mechanism. FASEB J. 22, 677.3.
*McGill, M.A., *O’Brien, K.M., and Mills, K.V. (2008) The P. abyssi lon-b protease:
Protein splicing activity and proteolysis by soluble fragments. Abstracts of the ASBMB
2008 National Meeting.
*Kerrigan, A.M., *Powers, T.L., *Dorval, D.M., Reitter, J.N. and Mills, K.V. (2008) The
dependence of three P. abyssi inteins on extein sequence for efficient protein splicing.
FASEB J. 20, 611.13.
Mills, K.V., Reitter, J.N., Connor, K.R., *Dorval, D.M., *Drago, M.J., *Lewandowski,
K.T., and *Powers, T.L. (2008) Alternate mechanisms of protein splicing by noncanonical inteins. Abstracts of the ASBMB 2008 National Meeting.
Mills, K.V., Reitter, J.N., Connor, K.R., *Powers, T.L. and *Drago, M.J. (2007) Protein
splicing of a non-canonical intein from C. thermocellum. Abstracts of Papers, 234th ACS
National Meeting, Boston, MA, BIOL-237.
*McGill, M.A., *O’Brien, K.M., and Mills, K.V. (2007) Protein splicing and extein
activity of the Pyrococcus abyssi lon protease intein. Protein Science 16-Sup 1, 162.
Mills, K.V., Reitter, J.N., Connor, K.R., *Powers, T.L., *Drago, M.J., *Lewandowski,
K.T., and *Dorval, D.M. (2007) Alternative protein splicing mechanisms of inteins from
C. thermocellum and P. abyssi. Protein Science 16-Sup 1, 158.
*Dorval, D.M., Connor, K.R., and Mills, K.V. (2006) Catalysis of individual steps of
protein splicing of the Pyrococcus abyssi PolII intein. FASEB J. 20, A40.
*Powers, T.L., *Drago, M.J., *Dorval, D.M., Connor, K.R., and Mills, K.V. (2006)
Protein splicing of a non-canonical Clostridium thermocellum intein with N-terminal Gln.
FASEB J. 20, A964.
Mills, K.V., *Lewandowski, K.T., and *Dorval, D.M. (2005) The Mechanism of Protein
Splicing of the Pyrococcus abyssi PolII intein. Protein Science 14-Sup 1, 87.
*Lewandowski, K.T., *Dorval, D.M., and Mills, K.V. (2005) A Kinetic study of the
protein splicing mechanism of the Pyrococcus abyssi PolII intein. FASEB J. 19, A1401.
11
Mills, K., *Manning, J., *Garcia, A., *Wuerdeman, L. (2004) In vitro protein splicing of
an intein with a C-terminal glutamine from Pyrococcus abyssi. FASEB J. 18, C276.
*Lewandowski, K., *Wuerdeman, L., *Twomey, P., and Mills, K. (2004) Murine Sonic
Hedgehog proteins: Sub-cloning, over-expression in E. coli and affinity purification.
FASEB J. 18, C277.
Invited Research Seminars
Loyola University, Department of Chemistry, Baltimore, MD, February 10, 2015
Biophysical Society 59th Annual Meeting, Baltimore, MD, February 8, 2015
FASEB/ASBMB National Meeting 2014, San Diego, CA, April 27, 2014
Union College, Department of Chemistry, Schenectady, NY, October 11, 2012
Wellesley College, Biochemistry, Wellesley, MA, September 19, 2012
FASEB/ASBMB National Meeting 2010, Anaheim, CA, April 28, 2010
Pomona College/Harvey Mudd College, Claremont, CA, April 27, 2010
University of Massachusetts, Department of Chemistry, Amherst, MA, March 4, 2010
University of Vermont, School of Medicine, Burlington, VT, April 17, 2009
Trinity College, Hartford, CT, May 30, 2008
Worcester Polytechnic Institute, Worcester, MA October 12, 2005
Clark University, Worcester, MA April 1, 2002
New England Biolabs, Beverly, MA October 11, 2001
Gordon Research Conference, Biological Regulation Mechanisms
Holderness, NH, July 30-August 4, 2000
Other Selected Professional Presentations, Poster Presentations
FASEB/ASBMB 2015, Boston, MA, March 29, 2015
Winter Enzyme Mechanism Conference, Galveston, TX, January 5, 2015
28th Symposium of the Protein Society, San Diego, CA, July 29, 2014
12
FASEB/ASBMB 2014, San Diego, CA, April 26, 2014
27th Symposium of the Protein Society, Boston, MA July 20, 2013
FASEB/ASBMB 2013, Boston, MA, April 23, 2013
25th Anniversary Symposium of the Protein Society, Boston, MA July 26, 2011
FASEB/ASBMB 2011, Washington, DC, April 10, 2011
Dreyfus Foundation, New York, NY, October 30, 2010
FASEB/ASBMB 2010, Anaheim, CA, April 25, 2010
23rd Symposium of the Protein Society, Boston, MA, July 25-29, 2009
Gordon Research Conference, Enzymes, Coenzymes and Metabolic Pathways
University of New England, Biddeford, ME, July 2008
IUBMB/ASBMB 2008, San Diego, CA April 8, 2008
The 234th American Chemical Society National Meeting, Boston, MA, August 21, 2007
Twenty-First Symposium of the Protein Society, Boston, MA July 21-July 25, 2007
Nineteenth Symposium of the Protein Society, Boston, MA July 30-August 3, 2005
IUBMB/ASBMB 2004, Boston, MA June 15, 2004
Gordon Research Conference, Biological Regulation Mechanisms
Holderness, NH, July 30-August 4, 2000
Thirteenth Symposium of the Protein Society, Boston, MA, July 24-28, 1999
Student Research Mentoring
Summer Research Students
Summer 2015
Alexander Bonano (Chemistry ’17)
Christopher Cousin (Chemistry ’16)
Joshua Long (Biology ’17)
Christopher Minteer (Chemistry/Biochemistry ’17)
Isabelle Pierre (UMass-Dartmouth, ’15)
Christian Ramsoomair (Chemistry ’18)
13
Summer 2014
Alexander Bonano (Chemistry ’17, NSF)
Dawilmer Castillo (’15, Bridgewater State NSF)
Michael Koulopoulos (Chemistry/Biochemistry ’16, Holy Cross)
Zachary Giaccone (Chemistry/Biochemistry ’15, NSF)
Mario Jaramillo (Biology ’15, NSF)
Anthony Yakely (Chemistry/Biochemistry ’16, Dreyfus)
Summer 2013
Julliard del Rosario, ’16 Cornell University (NSF)
Zachary Giaccone (Chemistry/Biochemistry ’15)
Daniel Haley (Chemistry ‘14)
Mario Jaramillo (Biology ’15)
Nicolle Siegart (Biology/Biochemistry ’15)
Summer 2012
Sarah Cahn (Chemistry ’14, Becton Dickinson)
Kathryn Colelli (Biology/Biochemistry ’13, Beckman Foundation)
Nick Gulbrandsen (Chemistry ’13, Fitchburg State)
Jennifer Pusztay (Chemistry/Biochemistry ’13, NSF)
Nicolle Siegart (Biology ’15, NSF)
Summer 2011
Kathryn Colelli (Biology/Biochemistry ’13, Beckman Foundation)
Kevin Karanja (Worcester State Chemistry, ’12, NSF)
Michael Nicastri (Chemistry/Biochemistry ’13, Holy Cross)
Laura Urbanski (Chemistry/Biochemistry ’13, Holy Cross)
Jennie Williams (Chemistry ‘13, NSF)
Summer 2010
Stacy Chin (Chemistry ’12, NSF)
Colleen Donahue (Biology ’11, NIH)
Michelle Marieni (Biology ’11, NSF)
George Savidis (Biology/Biochemistry ’12, Dreyfus)
Kristina Xega (Chemistry/Biochemistry ’11, NSF)
Summer 2009
Lauren Duffee (Biology/Biochemistry ’10, NSF)
Adam Nadelson (Biology ’10, NSF)
Kevin O’Brien (Biology/Biochemistry ’11, NSF)
Daniel York (Biology/Biochemistry ’10, NSF)
Summer 2008
Adam Nadelson (Biology ’10, NSF)
Daniel York (Biology/Biochemistry ’10, NSF)
14
Summer 2007
Adam Kerrigan (Biology ’09, NSF)
Melissa McGill (Biology/Biochemistry ’08, NSF)
Ann Schufreider (Chemistry ’09, NSF)
Jennifer Winslow (Biology ’09, NSF)
Summer 2006
Deirdre M. Dorval (Biology ’06, NSF)
Melissa McGill (Biology/Biochemistry ’08, NSF)
Kathryn O’Brien (Chemistry/Biochemistry ’08, NSF)
Summer 2005
Matthew Drago (Biology/Biochemistry ’07, NSF)
Deirdre M. Dorval (Biology ’06, Research Corporation)
Taryn Powers (Biology ’07, NSF)
Summer 2004
Deirdre M. Dorval (Biology ’06, Research Corporation)
Katherine Lewandowski (Biology/Biochemistry ’05, Simeon J. Fortin Charitable Trust)
Summer 2003
Jaclyn Gareau (Chemistry/Biochemistry ’04, Simeon J. Fortin Charitable Trust)
Katherine Lewandowski (Biology/Biochemistry ’05, Simeon J. Fortin Charitable Trust)
Summer 2002
Alicia M. Garcia (Biology ’03, Simeon J. Fortin Charitable Trust)
Jennifer S. Manning (Chemistry ’03, Simeon J. Fortin Charitable Trust)
Timothy D. Quinn (Chemistry/Biochemistry ’04, Pfizer, PREPARE)
Lisa A. Wuerdeman (Chemistry ’03, Simeon J. Fortin Charitable Trust)
Academic Year Research Students
2015-2016
Christopher Cousin (Chemistry ’16)
Chemistry Research I
Michael Koulopoulos (Chemistry/Biochemistry ’16)
Chemistry Research II/Biochemistry Thesis
Joshua Long (Biology ’17)
Chemistry Research I
Christopher Minteer (Chemistry/Biochemistry ’17)
Chemistry Research I
Christian Ramsoomair (Chemistry ’18)
Introduction to Research
Anthony Yakely (Chemistry/Biochemistry ’16)
15
Chemistry Research II/Biochemistry Thesis
2014-2015
Alexander Bonano (Chemistry ‘17)
ALANA Mentoring Program
Vanessa Costa (UNDL ’18)
First year Research Advancement Program
Christopher Cousin (Chemistry ’16)
Introduction to Research
Zachary Giaccone (Chemistry/Biochemistry ’15)
Chemistry Research II/Biochemistry Thesis
Michael Koulopoulos (Chemistry/Biochemistry ’16)
Chemistry Research I
Mario Jaramillo (Biology ’15)
Chemistry Research II
Christian Ramsoomair (UNDL ’18)
First year Research Advancement Program
Nicolle Siegart (Biology/Biochemistry ’15)
Chemistry Research II/Biochemistry Thesis
Anthony Yakely (Chemistry/Biochemistry ’16)
Chemistry Research I
2013-2014
Alexander Bonano (Chemistry ‘17)
ALANA Mentoring Program
Sarah Cahn (Chemistry ’14)
Chemistry Research II
Zachary Giaccone (Chemistry/Biochemistry ’15)
Chemistry Research I
Daniel Haley (Chemistry ‘14)
Chemistry Research I
Mario Jaramillo (Biology ’15)
Chemistry Research I
Nicolle Siegart (Biology/Biochemistry ’15)
Chemistry Research I
2012-2013
Sarah Cahn (Chemistry ’14)
Chemistry Research I
Kathryn Colelli (Biology/Biochemistry ’13)
Chemistry Research II/Biochemistry Thesis
Michael Nicastri (Chemistry/Biochemistry ’13)
Chemistry Research II/Biochemistry Thesis
Jennifer Pusztay (Chemistry/Biochemistry ’13)
Chemistry Research II/Biochemistry Thesis
Nicolle Siegart (Biology ’15)
16
Introduction to Research
Laura Urbanski (Chemistry/Biochemistry ’13)
Chemistry Research II/Biochemistry Thesis/Honors Thesis
Jennie Williams (Chemistry ’13)
Chemistry Research II
2011-2012
Stacy Chin (Chemistry ’12)
Chemistry Research II
Kathryn Colelli (Biology/Biochemistry ’13)
Chemistry Research I
Kevin Karanja (Chemistry-Worcester State ’12)
Michael Nicastri (Chemistry/Biochemistry ’13)
Chemistry Research I
Jennifer Pusztay (Chemistry/Biochemistry ’13)
Introduction to Research
George Savidis (Biology/Biochemistry ’12)
Chemistry Research II/Biochemistry Thesis
Laura Urbanski (Chemistry/Biochemistry ’13)
Chemistry Research I
Jennie Williams (Biology ’13)
Introduction to Research
2010-2011
Stacy Chin (Chemistry ’12)
Chemistry Research I
Colleen Donahue (Biology ’11)
Chemistry Research I
Michelle Marieni (Biology ’11)
Chemistry Research I
Kevin O’Brien (Biology/Biochemistry ’11)
Chemistry Research II
George Savidis (Biology/Biochemistry ’12)
Chemistry Research I
Jennie Williams (Biology ’13)
Introduction to Research
Kristina Xega (Chemistry/Biochemistry ’11)
Chemistry Research I
2009-2010
Lauren Duffee (Biology/Biochemistry ’10)
Chemistry Research II/Biochemistry Thesis
Adam Nadelson (Biology ’10)
Chemistry Research II
Kevin O’Brien (Biology/Biochemistry ’11)
Chemistry Research I
17
Kristina Xega (Chemistry/Biochemistry ’11)
Introduction to Research
Daniel York (Biology/Biochemistry ’10)
Chemistry Research II/Biochemistry Thesis
2008-2009
Lauren Duffee (Biology/Biochemistry ’10)
Introduction to Research
Adam Kerrigan (Biology ’09)
Chemistry Research II
Adam Nadelson (Biology ’10)
Chemistry Research I
Ann Schufreider (Chemistry ’09)
College Honors
Jennifer Winslow (Biology ’09)
Chemistry Research II
Daniel York (Biology/Biochemistry ’10)
Chemistry Research I
2007-2008
Adam Kerrigan (Biology ’09)
Chemistry Research I
Melissa McGill (Biology/Biochemistry ’08)
Chemistry Research II/Biochemistry Thesis
Kathryn O’Brien (Chemistry/Biochemistry ’08)
Chemistry Research II/Biochemistry Thesis
Ann Schufreider (Chemistry ’09)
Chemistry Research I
Jennifer Winslow (Biology ’09)
Chemistry Research I
2006-2007
Matthew Drago (Biology/Biochemistry ’07)
College Honors/Biochemistry Thesis
Melissa McGill (Biology/Biochemistry ’08)
Chemistry Research I
Kathryn O’Brien (Chemistry/Biochemistry ’08)
Chemistry Research I
Taryn Powers (Biology ’07)
Chemistry Research II
2005-2006
Deirdre Dorval (Biology ’06)
College Honors
Matthew Drago (Biology/Biochemistry ’07)
Chemistry Research I
18
Taryn Powers (Biology ’07)
Chemistry Research I
2004-2005
Deirdre Dorval (Biology ’06)
Chemistry Research I
Katherine Lewandowski (Biology/Biochemistry ’05)
Chemistry Research II/Biochemistry Thesis
2003-2004
Jaclyn Gareau (Chemistry/Biochemistry ’04)
Chemistry Research II/Biochemistry Thesis/Departmental Honors
Kathleen Leahy (Mathematics ’04)
College Honors (on-campus advisor)
Katherine Lewandowski (Biology/Biochemistry ’05)
Chemistry Research I
Timothy Quinn (Chemistry/Biochemistry ’04)
Chemistry Research II/Biochemistry Thesis/Departmental Honors
2002-2003
Alicia Garcia (Biology ’03)
College Honors
Jaclyn Gareau (Chemistry/Biochemistry ’04)
Chemistry Research I
Timothy Quinn (Chemistry/Biochemistry ’04)
Chemistry Research I
Jennifer S. Manning (Chemistry ’03)
Chemistry Research I
Ralph M. Millilo (Biology ’03) with Prof. R. Bertin
Biology Research/Biochemistry Thesis
Lisa Wuerdeman (Chemistry ’03)
Chemistry Research I
2001-2002
Beth Ambrose (Chemistry ’02)
Chemistry Research I
Patrick Twomey (Chemistry/Biochemistry ’02)
Chemistry Research I/Biochemistry Thesis
Jennifer S. Manning (Chemistry ’03)
Unofficial Chemistry Research
Lisa Wuerdeman (Chemistry ’03)
Unofficial Chemistry Research
19
College Honors Program Thesis Reader
Katherine Beckwith (Biology ’03), 2003
Cristiana Pineda (Chemistry ’12), 2012
Selected College Service/Professional Development
20132013
2011-2013
2013
2013-2014
2013
2013
2011-2012
2008-2010
2008-2009
2008-2010
2008-2009
2008-2010
2010
2007
2004-2006
2004-2006
2001-present
2002-2003, 2004-2007
2002-2003
2006-07, 10-12
2002
2002-2004, 2007
2002-present
2004
2004-2006
2005
2003-2006, 2008
2002-2007
STEM-E working group, co-founder
Search Committee, Director of Sponsored Research
Finance and Planning Council
Independent Colleges Office Liaison Meeting participant
Colleges of Worcester Consortium Leadership Fellows
Program
Ad hoc Montserrat Writing Assessment Committee
Ad hoc AAC subcommittee on faculty research
Presidential Colloquium on Jesuits and the Liberal Arts
Committee on Faculty Affairs, Chair, 2009-10
Subcommittee Chair, Handbook on Tenure and Promotion
Subcommittee on Appeals
Science Ambassadors Faculty Advisor
Faculty mentorship program
NEASC Review Subcommittee on Governance
Faculty Mentorship Program
Beckman Scholars selection committee
“Being a Holy Cross Student” Gateways Session Leader
Academic Affairs Council, Priorities Committee
Curricular Review Steering Committee
Biochemistry Concentration, Co-Chair
Committee for Graduate Studies and Fellowships
Curricular Goals Committee: Acquisition, integration and
application of knowledge
Presidential Service Award Selection Committee
Undergraduate Summer Research Symposium, co-chair
Summer Gateways Advisor
Founder/organizer of summer biomolecular
summer student research student journal club
Presented talk on “Mad Cows and Cannibals” for
summer research program
PAL host for Atoms and Molecules
Ad hoc Classroom of the Future committee
Organized student group trip to MIT Center for Cancer
Research Annual Symposium
Founded/organized annual summer student/faculty softball
game series (w/ G. Roberts)
20
Selected Department Service
2009
2004-2010
2005-2007
Revised and lead department seminar program as teaching overload
General Chemistry laboratory faculty director
New Chemistry building planning, committees for Material Management,
General Chemistry lab, Biochemistry research and Non-synthetic
Research
2001-2002
Cross and Crucibles faculty advisor
2001,02,05.07 Participated in Fall Parent’s Weekend Open House
2004, 12
Hosted prospective student department session
2003,04,07,12 Hosted departmental Gateways browsing sessions
2001-present Hiring committees for ten faculty and four lab coordinators
2003
Co-wrote Departmental Academic Honesty Policy
2001
Graduate School advising session panelist
2001-present Biochemistry coordinator
2001-present Hosted prospective student summer visits to the department
Selected Community Service
2015
Panelist, Massachusetts STEM summit, Proven Strategies in Fostering and
Retaining STEM Students
2014
NSF Review Panel, Division of Undergraduate Education (Apr 2014)
2012-16
Wachusett Regional School Committee member
2012-2013: Subcommittees on Education and Community Outreach
2012-: Davis Hill Elementary School Improvement Council
2013: Subcommittees on Management and Legal Affairs (Chair)
2012
Colleges of Worcester Consortium, Faculty panelist, “Planning Successful
Scholarship: Obtaining Fellowships and Grants for Sabbaticals and
Ongoing Research” (11/2/12)
2011
PKAL Learning Spaces Collaboratory Regional Workshop Panelist
2011
NSF Review Panel, Molecular and Cellular Biosciences (Apr 2011)
2010
NSF Review Panel, Molecular and Cellular Biosciences (Apr 2010)
2010
Chemistry demonstration to 50 4-6 year olds, Goddard School, Auburn,
MA
2010,11,14 Poster judge at undergraduate poster session, ASBMB national meeting
2009
NSF Review Panel MRI-PUI, Chemistry (Oct 2009)
2007Ad hoc reviewer for Protein Science, Biochemistry, National Science
Foundation MCB, Chembiochem, Journal of Chemical Education, IUBMB
Life, BMC Bioinformatics, Journal of Bacteriology, PLoS One, Acta
Biochimica et Biophysica Sinica, Nature Biotechnology, Nature Chemical
Biology, Frontiers in Microbiology
2009
Poster session judge at Protein Society meeting
2008-present Founder and Advisor, Holy Cross Undergraduate Affiliate Network of
American Society for Biochemistry and Molecular Biology
2006
Seminar to post-doc association at UMass Medical School on career
options in liberal arts colleges
21
2005
2005
2005
2001-2002
2002
Participated as panelist in Science faculty forum by Consortium on High
Achievement and Success, Mt. Holyoke College
Presented talk to Johns Hopkins Center for Talented Youth Odyssey
Series
Panelist for HHMI-Boston area alumni group career options discussion
United Way Fund Drive Department Coordinator
Relay for Disaster Relief participant
Professional Membership
2008-present
2000-present
2000-present
2005-present
2001-present
1995-present
1995-present
2010-present
Project Kaleidoscope, F21
American Chemical Society, Biological Chemistry Division
American Society for Biochemistry and Molecular Biology
Protein Society
Council on Undergraduate Research
Phi Beta Kappa
Alpha Sigma Nu (National Jesuit Honor Society)
American Society for Microbiology
22