University of Windsor Great Lakes Institute for Environmental Research Comparison of Traditional Taxonomy and Molecular Techniques for Identifying Rare Species in Hamilton Harbour Keara Stanislawczyk MSc Candidate GLIER, University of Windsor Friday, February 28, 2014 at 3:00 pm, GLIER Conference Center, Room 250 Non-native species (NIS) including zebra mussel, round goby, and fishhook waterflea, among others, have been changing the dynamics of the Great Lakes since their introductions. These NIS have negative environmental and economic impacts on their new environment, and are difficult to eliminate. Many of these NIS were introduced and moved throughout the Great Lakes via ballast water discharged from both domestic and international ships. When a species is first introduced, the population size will generally be very small. This is the best time to eliminate the NIS; however, it is difficult to detect rare species in aquatic systems. Two techniques for detecting species, traditional taxonomy, and environmental genomics (454 pyrosequencing), were compared for their effectiveness in detecting Cladocera zooplankton in Hamilton Harbour, Ontario. My thesis will utilize traditional taxonomy to validate rare species previously detected using genetic methodologies. This study will provide insight regarding the use of each of these two techniques, and will help scientists choose the most appropriate method for their studies.
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