MATERIAL SAFETY DATA SHEET
Cat# K812-1000 BCA Protein Quantification Kit
MSDS DATE: 9/3/2010
SECTION 1: PRODUCT AND COMPANY IDENTIFICATION
PRODUCT NAME:
BCA Protein Quantification Kit
PRODUCT CODES:
Cat# K812-1000
MANUFACTURER:
DIVISION:
ADDRESS:
BioVision, Inc.
980 Linda Vista Avenue, Mountain View, CA 94043
EMERGENCY PHONE: 858-373-8066
CHEMTREC PHONE:
OTHER CALLS:
650-428-0236
FAX PHONE:
650-428-0336
SECTION 2: COMPOSITION/INFORMATION ON INGREDIENTS
Component
CAS
Number
Concentration
MW
Chemical Formula
BCA Reagent contains:
Bicinchoninic Acid (Na salt)
Sodium Carbonate
Copper Sulfate
BSA Standard
N/A
5968-11-6
7758-99-8
9048-46-8
1-3 %
1-3%
4%
0.5%
388.3
124.0
249.7
66 kDa
C20H10N2Na2O4 •xH2O
Na2CO3•H2O
CuSO4•5H2O
N/A
Sodium Azide
26628-22-8
0.01%
65.01
NaN3
Safety Information
No hazardous or toxic properties
known
See below
The BSA (Bovine Serum Albumin)
originates from USA where BSE has
not been reported.
See Below
NOTE: The safety data shown below is based on pure ingredients: The amount in this kit is far less and comprises only 110 µl of
0.01% Sodium azide; 25 ml containing 4% Copper sulfate.
SECTION 3: HAZARDS IDENTIFICATION
Component
Copper Sulfate
Emergency Overview
Redness and irritation of the eyes and skin can occur with acute exposure
Sodium Azide, NaN3
Highly toxic by ingestion and skin adsorption; Harmful if inhaled; May cause skin irritation can be fatal if
adsorbed through skin; Maycause eye irritation; May be fatal if swallowed
SECTION 4: FIRST AID MEASURES
•
•
•
•
•
After inhalation
Supply fresh air. If required, provide artificial respiration. If breathing is difficult, give oxygen.
After skin contact
Remove contaminated clothing, wash with water and soap and rinse thoroughly. May cause dermatitis.
After eye contact
Immediately rinse opened eye for several minutes under running water. Get medical attention.
After swallowing
Wash mouth out with plenty of water. Seek immediate medical advice.
Note to Physician:
Not available.
SECTION 5: FIRE-FIGHTING MEASURES
•
•
Suitable extinguishing agents
Carbon dioxide, water spray, extinguishing powder or appropriate foam.
Special fire fighting procedure
None needed.
PAGE 1 OF 5
MATERIAL SAFETY DATA SHEET
Cat# K812-1000 BCA Protein Quantification Kit
•
MSDS DATE: 9/3/2010
Protective equipment
Wear self-contained respirator.
SECTION 6: ACCIDENTAL RELEASE MEASURES
•
•
•
Personal precautions
Wear protective equipment. Keep unprotected persons away. Ensure adequate ventilation.
Small spill and leak
Use appropriate tools to collect the spilled solid in a waste disposal container.
Clean with water on the contaminated surface.
Dispose of according to local and regional authority requirements.
Large spill and leak
Stop leak if without risk. Do not touch damaged container or spilled item.
Inform supervisor or the head of safety department. Cover spilled item with disinfectant.
Clean with water on the contaminated surface.
SECTION 7: HANDLING AND STORAGE
•
Handling
Keep container tightly sealed.
Store in at 4 °C, short term; -20 °C Long term
•
Storage
Keep container tightly closed. Store as directed on product insert.
SECTION 8: EXPOSURE CONTROLS/PERSONAL PROTECTION
•
Hygienic measures
Follow the usual precautionary measures for handling chemicals.
Wear suitable protective equipment and clothing.
Keep away from food and beverages.
Remove all soiled and contaminated clothing immediately.
Avoid contact with skin and eyes.
Wash hands thoroughly after handling.
•
Personal protective equipment
Use suitable respirator when high concentration are present.
Wear lab coat, gloves and splash goggles.
SECTION 9: PHYSICAL AND CHEMICAL PROPERTIES
Property
Physical state:
Color:
Molecular Weight:
Solubility:
Flash point:
Copper Sulfate
Crystalline
blue
249.7 g/mol
soluble
Not applicable
Sodium Azide
Crystalline
white
65.01 g/mol
Partially soluble in cold water
Not applicable
SECTION 10: STABILITY AND REACTIVITY
Property
Stability
Avoid
Hazardous
decomposition
Copper Sulfate
Stable under normal storage
Powdered metals; reacts violently
with hydroxylamine, magnesium
Sodium Azide
Stable under normal storage
Halogenated hydrocarbon,
metals, acids, acid chlorides
Copper oxides, sulphur oxides
Sodium oxides
PAGE 2 OF 5
MATERIAL SAFETY DATA SHEET
Cat# K812-1000 BCA Protein Quantification Kit
MSDS DATE: 9/3/2010
SECTION 11: TOXICOLOGICAL INFORMATION
Copper Sulfate:
RTECS: GL8900000
Acute toxicity
LD50 Oral - rat - 300 mg/kg
Remarks: anhydrous
LD50 Dermal - rat - > 2,000 mg/kg
Remarks: anhydrous
Skin corrosion/irritation: no data available
Serious eye damage/eye irritation: no data available
Respiratory or skin sensitization: Prolonged or repeated exposure may cause allergic reactions in certain sensitive individuals.
Germ cell mutagenicity: no data available
Carcinogenicity
IARC: No component of this product present at levels greater than or equal to 0.1% is identified as probable,
possible or confirmed human carcinogen by IARC.
ACGIH: No component of this product present at levels greater than or equal to 0.1% is identified as a
carcinogen or potential carcinogen by ACGIH.
NTP: No component of this product present at levels greater than or equal to 0.1% is identified as a known or
anticipated carcinogen by NTP.
OSHA: No component of this product present at levels greater than or equal to 0.1% is identified as a
carcinogen or potential carcinogen by OSHA.
Reproductive toxicity: no data available
Specific target organ toxicity - single exposure (GHS): no data available
Specific target organ toxicity - repeated exposure (GHS): no data available
Aspiration hazard: no data available
Sodium Azide:
RTECS: VY8050000
Acute toxicity
LD50 Oral - rat - 27 mg/kg
LC50 Inhalation - rat - 37 mg/m3
Remarks: Sense Organs and Special Senses (Nose, Eye, Ear, and Taste):Eye:Other. Behavioral:Convulsions or effect
on seizure threshold. Lungs, Thorax, or Respiration:Structural or functional change in trachea or bronchi.
LD50 Dermal - rabbit - 20 mg/kg
Irritation and corrosion
no data available
Sensitisation
no data available
SECTION 12: ECOLOGICAL INFORMATION
Copper Sulfate:
Toxicity
Toxicity to daphnia and other aquatic invertebrates.
EC50 - Daphnia magna (Water flea) - 0.024 mg/l - 48 h
Persistence and degradability: no data available
Bioaccumulative potential: no data available
Mobility in soil: no data available
PBT and vPvB assessment: no data available
Other adverse effects: An environmental hazard cannot be excluded in the event of unprofessional handling or disposal.
Very toxic to aquatic life.
Sodium Azide:
Elimination information (persistence and degradability): no data available
Ecotoxicity effects
Toxicity to fish LC50 - Lepomis macrochirus - 0.68 mg/l - 96 h
Toxicity to daphnia and other aquatic invertebrates.
EC50 - Daphnia pulex (Water flea) - 4.2 mg/l - 48 h
Further information on ecology: An environmental hazard cannot be excluded in the event of unprofessional handling or disposal.
Very toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environment.
no data available
PAGE 3 OF 5
MATERIAL SAFETY DATA SHEET
Cat# K812-1000 BCA Protein Quantification Kit
MSDS DATE: 9/3/2010
SECTION 13: DISPOSAL CONSIDERATIONS
Recommendation
Disposal must be made in accordance with federal, state and local environmental regulations.
SECTION 14: TRANSPORT INFORMATION
Copper Sulfate:
DOT (US)
UN-Number: 3077 Class: 9 Packing group: III
Proper shipping name: Environmentally hazardous substances, solid, n.o.s. (Copper sulphate pentahydrate)
Reportable Quantity (RQ): 10 lbs
Marine pollutant: Marine pollutant
Poison Inhalation Hazard: No
IMDG
UN-Number: 3077 Class: 9 Packing group: III EMS-No: F-A, S-F
Proper shipping name: ENVIRONMENTALLY HAZARDOUS SUBSTANCE, SOLID, N.O.S. (Copper sulphate
pentahydrate)
Marine pollutant: Marine pollutant
IATA
UN-Number: 3077 Class: 9 Packing group: III
Proper shipping name: Environmentally hazardous substance, solid, n.o.s. (Copper sulphate pentahydrate)
Further information
EHS-Mark required (ADR 2.2.9.1.10, IMDG code 2.10.3) for single packagings and combination packagings containing
inner packagings with Dangerous Goods > 5L for liquids or > 5kg for solids.
Sodium Azide:
DOT (US)
UN-Number: 1687 Class: 6.1
Proper shipping name: Sodium azide
Reportable Quantity (RQ): 1000 lbs
Marine pollutant: No
Poison Inhalation Hazard: No
IMDG
UN-Number: 1687 Class: 6.1
Proper shipping name: SODIUM AZIDE
Marine pollutant: No
IATA
UN-Number: 1687 Class: 6.1
Packing group: II
Packing group: II
EMS-No: F-A, S-A
Packing group: I
SECTION 15: REGULATORY INFORMATION
Copper Sulfate:
OSHA Hazards: Target Organ Effect, Toxic by ingestion, Irritant
DSL Status: All components of this product are on the Canadian DSL list.
SARA 302 Components
SARA 302: No chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302.
SARA 313 Components
Copper sulphate pentahydrate
SARA 311/312 Hazards
Acute Health Hazard, Chronic Health Hazard
California Prop. 65 Components
This product does not contain any chemicals known to State of California to cause cancer, birth, or any other reproductive defects.
Sodium Azide
OSHA Hazards: Target Organ Effect, Highly toxic by ingestion, Highly toxic by skin absorption
DSL Status: All components of this product are on the Canadian DSL list.
SARA 302 Components
Sodium azide
CAS-No.26628-22-8
Revision Date 2007-07-01
SARA 313 Components
Sodium azide
CAS-No.26628-22-8
Revision Date 2007-07-01
SARA 311/312 Hazards
Acute Health Hazard, Chronic Health Hazard
California Prop. 65 Components
This product does not contain any chemicals known to the State of California to cause cancer, birth, or any other
reproductive defects.
PAGE 4 OF 5
MATERIAL SAFETY DATA SHEET
Cat# K812-1000 BCA Protein Quantification Kit
EU regulations
Component
Copper sulfate
Sodium azide
MSDS DATE: 9/3/2010
Risk Phrases
R52/53
R22/31
Safety Phrases
S61
S28/36/45
SECTION 16: OTHER INFORMATION
OTHER INFORMATION:
PREPARATION INFORMATION:
DISCLAIMER:
The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a
guide. The information in this document is based on the present state of our knowledge and is applicable to the
product with regard to appropriate safety precautions. It does not represent any guarantee of the properties of the
product. BioVision, Inc., shall not be held liable for any damage resulting from handling or from contact with the
above product. See reverse side of invoice or packing slip for additional terms and conditions of sale.
PAGE 5 OF 5