What’s new in Spinsolve v1.3?
New 2D Carbon-Proton correlation protocols
Spinsolve v1.3 includes several protocols for advanced two-dimensional proton-carbon
correlation experiments, using direct as well as indirect carbon detection. The acquisition is
made very easy, the user only needs to select the number of scans and the repetition time
from a dropdown box.
The following protocols are available:

HETCOR (Heteronuclear Correlation): This protocol performs a two-dimensional
experiment to measure 1H and 13C nuclei that are that are connected directly to each
other. The two-dimensional spectrum displays the 13C spectrum along the direct (f2)
dimension and the 1H spectrum along the indirect (f1) dimension. Cross-peaks in the
spectrum reveal which 13C peak is correlated to which 1H peak via a one bond
coupling.

HMBC (Heteronuclear Multiple Bond Correlation): This protocol performs a twodimensional experiment to measure 1H and 13C nuclei that are that are correlated via
long-range coupling, usually over two or three bonds. The two-dimensional spectrum
displays the 1H spectrum along the direct (f2) dimension and the 13C spectrum along
the indirect (f1) dimension. Cross-peaks in the spectrum reveal which 13C peak is
correlated to which 1H peak via a multiple bond coupling.

HMQC (Heteronuclear Multiple Quantum Coherence): This protocol performs a twodimensional experiment to measure 1H and 13C nuclei that are that are connected
directly to each other. The two-dimensional spectrum displays the 1H spectrum along
the direct (f2) dimension and the 13C spectrum along the indirect (f1) dimension.
Cross-peaks in the spectrum reveal which 13C peak is correlated to which 1H peak via
a one bond coupling.

HSQC (Heteronuclear Single Quantum Coherence): This protocol performs a twodimensional experiment to measure 1H and 13C nuclei that are that are connected
directly to each other. The two-dimensional spectrum displays the 1H spectrum along
the direct (f2) dimension and the 13C spectrum along the indirect (f1) dimension.
Cross-peaks in the spectrum reveal which 13C peak is correlated to which 1H peak via
a one bond coupling.
New 2D Fluorine protocols
Spinsolve v1.3 includes several protocols for advanced two-dimensional fluorine
experiments. The acquisition is made very easy, the user only needs to select between Quick
(1 scan) and Power (4 scans) mode.
The following protocols are available:

F-COSY (Fluorine COSY): This protocol records a two-dimensional NMR spectrum
with the signals of a normal 1D spectrum correlated with each other. Cross-peaks
appear in the 2D spectrum if the corresponding fluorine nuclei couple with each other.
This provides information about chemical structure and connectivity of the molecule.
The pulse sequence is similar to the proton COSY and consists of two 90 degree rf
pulses separated by a variable delay.

F-JRES (Fluorine JRES): This protocol records a two-dimensional homonuclear jresolved fluorine spectrum. In a normal 1D spectrum, the peak splitting results from
the combination of chemical shift and j-coupling, and it may be difficult to assign
peaks in situations of overlapping multiplets. In the homonuclear 2D j-resolved
experiment, these two couplings are separated and displayed on different axes of the
2D spectrum. The pulse sequence is similar to the proton JRES and consists of a 90
degree excitation pulse followed by a 180 degree refocussing pulse. The echo signal is
acquired with the echo time being incremented.

FH-COSY (Fluorine-Proton COSY): This protocol records a heteronuclear twodimensional NMR spectrum with the signals of a normal 1D spectrum correlated with
each other. Cross-peaks appear in the 2D spectrum if the corresponding protons and
fluorine nuclei couple with each other. This provides information about chemical
structure and connectivity of the molecule.
New Phosphorus-31 capabilities
Spinsolve is now available as SpinsolvePhosphorus to record 31P spectra.

Phosphorus and Phosphorus+: These two protocols enable the acquisition of protondecoupled 31P spectra. The Phosphorus+ protocol offers more choice in adjustment of
experimental parameters compared to the simple to use Phosphorus protocol. Proton
spectra can be recorded of the same sample.
New or improved processing options

Resolution enhancement
The resolution enhancement feature is now available in the Proton+, T1, T2 and RM
protocols, if the acquisition time is set to 3.2 or 6.4 seconds.

Calibrate proton ppm scale
The proton ppm reference can be set manually, for example from a solvent peak.

Integration
This processing option allows the measurement of peak integrals.
Shimming
The Quickshim and Powershim have been improved.
Quickshim now uses a more intelligent algorithm to find the
optimal values. As a result, the number of iterations and total
duration may vary. It will usually take two minutes for a
Quickshim, but if extra iterations are needed, it may take up to five minutes.
Powershim also uses a more intelligent algorithm, but the total duration has not changed
significantly compared to v1.2.