牛乳中のアフラトキシンM1の分析
Analysis of aflatoxin M1 in milk
D150721B
Procedure
O
OH
Filtration
Purification
Measurement
O
O
O
H O
O
CH3
Aflatoxin M1
YMC-Triart C18 (5 µm, 250 X 4.6 mmI.D.)
Injection : 100 µL
peak area
Standard
40 ng/mL
2500000
20 ng/mL
400000
200000
10 ng/mL
2000000
0
0
10
4 ng/mL
20
30
40
50
concentration (ng/mL)
2 ng/mL
1500000
AFM1
R² = 0.9999
600000
Matrix standard (10 ng/mL)
600000
1000000
400000
500000
200000
0
0
5
10
15
20
25
min
30
YMC-Triart C18 (5 µm, 250 X 4.6 mmI.D.)
0
5
10
15
20
25
min
30
YMC-Triart C18 (3 µm, 100 X 3.0 mmI.D.)
Standard
Matrix standard
140000
0
Standard
Matrix standard
350000
100000
250000
60000
150000
20000
50000
0
5
-20000
10
15
20
25
30
min
-50000
00
5
10
Courtesy of Japan Frozen Foods Inspection Corporation
Column
Eluent
Flow rate
Temperature
Detection
Injection
: YMC-Triart C18 (12 nm)
: acetonitrile/water (25/75)
: 1.0 mL/min for 4.6 mmI.D., 0.8 mL/min for 3.0 mmI.D.
: 40℃
: FLS at Ex 365 nm, Em 435 nm
: 20 µL
min