Environmental Health and Safety Standard Operating Procedure Cleaning Nickel (Ni) Columns
Department: Chemistry & Chemical Biology
Date SOP was written: July 1, 2014
Date SOP was approved by PI/lab supervisor: Andy LiWang
Reviewed by: Andy LiWang
Principal Investigator: Andy LiWang
Internal Lab Safety Coordinator/Lab Manager:
Lab Phone: 209.228.4630
Office Phone: 209.228.4631
Emergency Contact: Karen Smith
209.205.8176
Location(s) covered by this SOP: Castle 1201
Suite 920
Type of SOP:
X Process
X Hazardous Chemical
☐
Hazardous Class
Purpose
This SOP covers the cleaning of Nickel (Ni) columns.
Table of Hazard Properties of Materials Used in This SOP:
Chemical Name
Sodium hydroxide (NaOH)
CAS #
1310-73-2
Health Hazards
Physical Hazards
- Corrosive
Not flammable or reactive
Guanidine hydrochloride
(GHCL)
50-01-1
- Irritant
Not flammable or reactive
Acetic acid
64-19-7
- Corrosive
Combustible liquid
Ethylenediaminetriacetic acid
(EDTA)
60-00-4
- Irritant
Not flammable or reactive
- Carcinogen
- Reproductive toxin
- Acutely Toxic
Not flammable or reactive
Nickel sulfate (NiSO4)
Cleaning Nickel (Ni) Columns
UCM- EH&S
7786-81-4
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Date: 07/01/2014
Written By/Reviewed By:
Environmental Health and Safety Summary of Significant Health and Physical Hazards
- Sodium hydroxide is a corrosive liquid, lab coat, gloves and eye goggles are required.
- Nickel sulfate is a carcinogen, reproductive toxin, and acutely toxic material. Lab coat, gloves and eye
goggles are required.
Personal Protective Equipment (PPE) Required to Handle Nickel (Ni) Column
- Gloves
- Safety Eye Goggles
- Lab Coats
- Close-toe shoes
Special Handling and Waste Storage/Disposal Requirements for Liquid That
Contain Nickel (Ni)
Nickel are considered ‘Particularly Hazardous Substances’ and need to be collected in a waste container
designated at the chemical fume hood in Andy LiWang’s lab.
Handling: Do not dump down the sink drain. Always pour liquid that contains Nickel (Ni) into a
designated waste container, then rinse with extra water and pour into waste container again to
make sure that all residual nickel (Ni) is collected in the waster collector container.
Waste Storage: Always collect in the designated waste container labeled Nickel (Ni) waste in the
chemical fume hood in Andy LiWang’s lab. Also put the Nickel (Ni) waste container in a
secondary containment.
Generate Waste container tag to put on waste container: using the Online Tag Program
http://otp.ucop.edu/ to generate waste container tag to put on waste container soon as
the first drop of waste is added to the container.
Waste Disposal: When the waste container for liquid that contains Nickel (Ni) is full, call EH&S at 2287864, or schedule online for pickup. Or EH&S will regularly pick chemical waste
automatically when waste tag expires at 90 days.
Risk Assessment
The overall health and safety risk for use of this material in accordance with the procedure and protocol in
the following section is considered LOW based on:
- The total quantity of any potentially hazardous material handled/transferred any one time is small.
Cleaning Nickel (Ni) Columns
UCM- EH&S
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Date: 07/01/2014
Written By/Reviewed By:
Environmental Health and Safety - Personnel wear protective clothing to prevent skin contact from splashes and for proper clean up
practices.
- Materials used in this SOP are not flammable or reactive.
Protocol/Procedure
Preparatory Steps:
- Review MSDS; special handling, decontamination, and waste disposal information in this SOP;
and the emergency information contained in this SOP.
- Visually verify that access to the emergency eyewash/shower unit and the fire extinguisher are not
blocked.
- Visually verify that access to the emergency exit door is free obstructions.
- Don the appropriate protective equipment.
Laboratory Procedure
Cleaning Ni Columns:
1. Let liquid drain out.
2. Fill column half full with 0.5 M Sodium hydroxide (NaOH), mix and soak for 30 mins, then drain.
3. Rinse full column with deionized water 3-5 times. Then Drain.
4. Rinse full column with 6M Guanidine Hydrochloride + 0.2M acetic acid solution 2 times. Drain.
5. Rinse full column with deionized water 3-5 times. Then Drain. Empty the liquid collector container
in the sink since no Nickel is present.
6. Fill column half full with 100mM Ethylenediaminetriacetic acid (EDTA) solution (pH 8), mix and
soak for 5 minutes. Drain. Now the liquid collector container has Nickel (Ni) and has to be
collected in the Nickel (Ni) waste.
7. Rinse full column with deionized water 3-5 times. Then Drain.
8. Fill column half full with 100mM Nickel sulfate (NiSO4) solution and store the column in this
solution until next use. Cleaning is done.
9. Pour all liquid that contain Nickel (Ni) into Nickel (Ni) waste container in the fume hood. Rinse the
column several times with water and collect the flowthrough and pour in to Nickel (Ni) waste
container to minimized Nickel residue.
In the event of an injury or chemical spill, perform first aid and call 911 and report
to Environmental Health and Safety at 209.228.4261.
Cleaning Nickel (Ni) Columns
UCM- EH&S
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Date: 07/01/2014
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Environmental Health and Safety Documentation of Training (signature of all users is required)
•
Prior to conducting any work with the chemicals listed above, designated personnel must provide
training to his/her laboratory personnel specific to the hazards involved in working with this substance,
work area decontamination, and emergency procedures.
•
The Principal Investigator must provide his/her laboratory personnel with a copy of this SOP and a
copy of the SDS provided by the manufacturer.
•
The Principal Investigator must ensure that his/her laboratory personnel have attended appropriate
laboratory safety training or refresher training within the last one year.
I have read and understand the content, requirements, and responsibilities of this SOP:
Name
Cleaning Nickel (Ni) Columns
UCM- EH&S
Signature
4
Date
Date: 07/01/2014
Written By/Reviewed By: