Thermo Scientific Pierce
HPLC Reagents
Monitor small molecules from synthesis to metabolization
Limited Time
Offer
Buy both TFA and FA –
for the price of TFA alone.
That’s a $70 value.
Details inside!
HPLC Ion Pairing Reagents
Trifluoroacetic Acid (TFA)
O
F3C
Trifluoroacetic acid is the most commonly used
ion pairing agent for use in reverse-phase HPLC
peptide separations.
Physical Properties
OH
•High purity and exceptional clarity – allows sensitive,
nondestructive peptide detection at low UV wavelengths
•High-performance packaging – TFA packaged under nitrogen
in amber glass ampules or bottles with protective PTFE-lined
fluorocarbon caps to ensure TFA integrity
•Convenience – 1mL ampules can be used to prepare 1L of
fresh 0.1% v/v trifluoroacetic acid solution for the mobile
phase in reverse-phase chromatography
Molecular Formula
CF3COOH
Molecular Weight
114.03g/mole
Density
1.53g/mL 20°C
Melting/Boiling Point
-15°C / 72°C
Alternative Names
Perfluoroacetic acid
Trifluoroethanoic acid
Trifluoracetic acid
CAS Number
76-05-01
Quality Specifications
Purity
>99.5%
Water Content
≤0.1%
Chain Length
≥99.5% C2
Ninhydrin’s Positives
A570≤0.02 above blank
Tollens Test
Negative for aldehyde content
UV absorbance for 0.1% aqueous solution
A280≤0.002
A254≤0.005
A230≤0.090
Ordering Information
Product #
PI-28901
Description
Trifluoroacetic Acid (TFA), Sequanal Grade
Pkg. Size
PI-28902
Trifluoroacetic Acid (TFA), Sequanal Grade Ampules
10x1g
PI-28903
Trifluoroacetic Acid (TFA), Sequanal Grade
100g
PI-28904
Trifluoroacetic Acid (TFA), Sequanal Grade Ampules
10x1mL
PI-28904BNDL
TFA and FA Ampule Bundle Pack
Kit
500mL
For making 0.1% w/v TFA solutions.
For making 0.1% v/v TFA solutions.
Available Nov. 1, 2010-Feb. 28, 2011, when you order using the PI-28904BNDL product #. Offer valid through
Feb. 28, 2011, or until inventory of specially marked packages are depleted. Not valid on previous purchases.
Cannot be combined with other offers.
Includes: Trifluoroacetic Acid (TFA) Ampules
Formic Acid (FA) Ampules
Special
Offer
Triethylamine (TEA)
H3C
Triethylamine is an ion-pairing reagent that
N
alters selectivity in reverse-phase HPLC
CH3
separations. By pairing with peptides, it
effectively sharpens peaks, resulting in improved
peak resolution. Thermo Scientific Pierce Triethylamine comes
in two grades:
•Ionate grade is designed for use as an ion-pair reagent in HPLC
separations and has a low UV absorbance to provide you with
the most sensitive detection across all wavelengths.
•Sequanal grade is designed to meet the special requirements
for peptide sequencing and analysis.
Ordering Information
Product #
PI-25108
Description
Triethylamine, Sequanal Grade
Pkg. Size
PI-53101
Triethylamine, Ionate Grade
25g
100g
10x1mL
10x1mL
Physical Properties
CH3
Molecular Formula
C6H15N
Molecular Weight
101.19g/mole
Density
0.726 g/mL
Alternate Names
TEA, Diethylethanamine
CAS Number
121-44-8
Quality Specifications – Ionate Grade
Particulates
Free
Purity
>99.5%
UV Analysis (0.1% Aqueous)
A280<0.005
A254<0.010
Quality Specifications – Sequanal Grade
Particulates
Free
Purity
>99.5%
Water Content
<0.2%
Ninydrin Positives
A570<0.20 above blank
Formic Acid (FA)
O
Formic acid is a common component of
HO
reverse-phase mobile phases that provide
protons for LC/MS analysis. The presence of a
low concentration of formic acid in the mobile
phase is also known to improve the peak shapes of the
resulting separation. Unlike trifluoroacetic acid (TFA),
formic acid is not an ion-pairing agent and it does not
suppress MS ionization of polypeptides when used as a
mobile-phase component.
Physical Properties
H
Molecular Formula
HCOOH (CH2O2)
Molecular Weight
46.02g/mole
Density
1.22g/mL
Flash Point
69°C
Freezing Point:
8°C
CAS Number
64-18-6
Quality Specifications
Purity
≥99%
Refractive Index:
1.3701-1.3721(20°C)
IR Spectrum
Only FA peaks
Visual
Clear iquid
Ordering Information
Product #
PI-28905
Description
Formic Acid, 99+%
10x1mL
PI-28904BNDL
TFA and FA Ampule Bundle Pack
Kit
Special
Offer
Pkg. Size
Available Nov 1, 2010-Feb. 28, 2011, when you order using the PI-28904BNDL product #. Offer valid through
Feb. 28, 2011, or until inventory of specially marked packages are depleted. Not valid on previous purchases.
Cannot be combined with other offers.
Includes: Trifluoroacetic Acid (TFA), Sequanal Grade for making 0.1% v/v TFA solutions
Formic Acid
Heptafluorobutyric Acid (HFBA)
Heptafluorobutyric acid is an ion pair
reagent for the reverse-phase HPLC
separation of proteins and peptides.
F F
CF3
Physical Properties
O
OH
F F
•Purity >99.5% HFBA purity and exceptional clarity
•High-performance packaging – HFBA packaged under
nitrogen in amber glass ampules or bottles
•Economical convenience – 1mL ampules can be used
to prepare 1 liter of fresh 0.1% heptafluorobutyric
acid solution for the mobile phase in reverse-phase
chromatography
10x1mL
10x1mL
Molecular Formula
C4HF7O2
Molecular Weight
214.04g/mole
Density
1.64g/mL
CAS Number
375-22-4
Quality Specifications
Purity
>99.5%
Water Content
<0.1%
Chain Length
>99.5% C4
UV (Neat)
A280<0.004 A254<0.006
A230<0.150
Sulfate
Negative
Ordering Information
Product # Description
PI-25003 Heptafluorobutyric Acid, Sequanal Grade
Pkg. Size
PI-53104 Heptafluorobutyric Acid, HPLC Grade
10x1mL
100mL
HPLC Solvents
Acetonitrile, LC/MS Grade
H
H C
Thermo Scientific Pierce LC/MS Grade Acetonitrile is
specially purified by a proprietary method and tested to
H
ensure lot-to-lot consistency with a low UV absorbance
to provide the most sensitive detection across all
wavelengths. Pierce Acetonitrile is 0.2 micron-filtered,
packaged in solvent-rinsed amber glass bottles and sealed under
a nitrogen atmosphere with PTFE-lined fluorocarbon caps for
ultimate protection.
Physical Properties
C
•We perform 37 quality tests to ensure low, stable baselines
and lot-to-lot consistency
•Low UV absorbance yields low baselines and high
detection sensitivity
•Low impurities protects columns and simplifies analysis
by eliminating extraneous peaks
N
Molecular Formula
CH3CN
Molecular Weight
41.05g/mole
Density
0.780g/mL
Alternative Names
Methyl cyanide, Cyanomethane,
Ethanenitrile
CAS Number
75-05-8
Quality Specifications
Purity (By GC)
99.9%
Water Content
≤0.01%
Residue after Evaporation
Titratable Acid, mEQ/g
Description
Acetonitrile, LC/MS Grade
≤0.0006
Trace Ionic Impurities
≤25ppb
Optical Absorbance
A190≤1
A200≤0.05
A215≤0.025
A225≤0.015
A254≤0.005
Ordering Information
Product #
PI-51101
≤0.8ppm
A195≤0.15
A210≤0.03
A220≤0.015
A230≤0.01
A280≤0.005
Pkg. Size
1L
Water, LC/MS Grade
Thermo Scientific Pierce LC/MS Grade Water is specially purified
by a proprietary method and tested to ensure lot-to-lot consistency
with a low UV absorbance to provide you with the most sensitive
detection across all wavelengths. Pierce Water is 0.2 micronfiltered, packaged in solvent-rinsed amber glass bottles and sealed
under a nitrogen atmosphere with Teflon® TFE-lined fluorocarbon
caps for ultimate protection.
Ordering Information
BN1004103 10/10 Printed in the U.S.
Product #
PI-51140
Description
Water, LC/MS Grade
Pkg. Size
1L
© 2010 Thermo Fisher Scientific Inc. All rights reserved. These products are supplied for
laboratory or manufacturing applications only. Prices listed herein were accurate at the time
of printing. Visit our website for up-to-date prices. Teflon is a trademark of E.I. du Pont de
Nemours and Company. All other trademarks are the property of Thermo Fisher Scientific Inc.
and its subsidiaries.
Physical Properties
Molecular Formula
H2O
Molecular Weight
18.01g/mole
CAS Number
7732-18-5
Quality Specifications
Protease
Not detected
Halogen Content
Not detected
Residue after Evaporation
≤1ppm
Trace Ionic Impurities
≤20ppb
Optical Absorbance
A210≤0.01
A230≤0.01
A254≤0.005
A280≤0.005
A220≤0.01
A240≤0.01
A260≤0.005