PRODUCT DATA SHEET
Pierce HeLa Protein Digest Standard, 5 × 20µg
Product Number: 88329
Summary:
The Thermo Scientific™ Pierce™ HeLa Protein Digest
Standard is a lyophilized, tryptic peptide mixture for
LC/MS qualitative performance testing. The digest is
specifically formulated for LC/MS experiments and
does not contain salts or detergents.
Applications:
Qualitative LC
LC/MS standardization
LC/MS MS method development
TMT™ reagent method development
Lot Number: PC199008
Source:
HeLa S3 cells
Visual:
White powder
Formulation:
20µg of lyophilized peptide mixture
Related Thermo Scientific Products:
88328 Pierce HeLa Protein Digest Standard,
20µg
Storage:
Shipped on cold packs. Store at -20°C or below.
88321 Pierce Peptide Retention Time
Calibration Mixture, 200µL
Stability:
Lyophilized product is stable for > 2 yrs at -20°C or
below. Reconstituted product is stable for up to 3
weeks at 4°C. For long term stability of reconstituted
product, aliquot and freeze at -80°C.
84840 Mass Spec Sample Prep Kit for
Cultured Cells, 20-reaction kit
Material Preparation:
Reconstitute product by dissolving in 40µL of 0.1%
formic acid in LC/MS grade water to a final
concentration of 0.5µg/µL.
Addition of 5% acetonitrile to reconstitution buffer may
facilitate dissolving of peptides but is not required.
Additional Information:
For MSDS please visit:
www.thermoscientific.com/pierce
Technical Support:
For Hardware-related support call:
1-800-532-4752
For Reagent support call:
1-800-874-3723
Products are warranted to operate or perform substantially in conformance with published Product specifications in effect at the time of sale, as set forth in the Product documentation, specifications and/or accompanying
package inserts (“Documentation”). No claim of suitability for use in applications regulated by FDA is made. The warranty provided herein is valid only when used by properly trained individuals. Unless otherwise stated in the
Documentation, this warranty is limited to one year from date of shipment when the Product is subjected to normal, proper and intended usage. This warranty does not extend to anyone other than Buyer. Any model or sample
furnished to Buyer is merely illustrative of the general type and quality of goods and does not represent that any Product will conform to such model or sample.
NO OTHER WARRANTIES, EXPRESS OR IMPLIED, ARE GRANTED, INCLUDING WITHOUT LIMITATION, IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR ANY PARTICULAR PURPOSE, OR NON
INFRINGEMENT. BUYER’S EXCLUSIVE REMEDY FOR NON-CONFORMING PRODUCTS DURING THE WARRANTY PERIOD IS LIMITED TO REPAIR, REPLACEMENT OF OR REFUND FOR THE NON-CONFORMING
PRODUCT(S) AT SELLER’S SOLE OPTION. THERE IS NO OBLIGATION TO REPAIR, REPLACE OR REFUND FOR PRODUCTS AS THE RESULT OF (I) ACCIDENT, DISASTER OR EVENT OF FORCE MAJEURE, (II)
MISUSE, FAULT OR NEGLIGENCE OF OR BY BUYER, (III) USE OF THE PRODUCTS IN A MANNER FOR WHICH THEY WERE NOT DESIGNED, OR (IV) IMPROPER STORAGE AND HANDLING OF THE PRODUCTS.
Unless otherwise expressly stated on the Product or in the documentation accompanying the Product, the Product is intended for research only and is not to be used for any other purpose, including without limitation,
unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses, or any type of consumption by or application to humans or animals. Preview is a trademark of Protein Metrics. TMT is a trademark
of Proteome Sciences plc. All other trademarks are the property of Thermo Scientific Inc. and its subsidiaries.
.
Pierce Biotechnology
PO Box 117
(800) 874-3723
(815) 968-7316 fax
3747 N. Meridian Road
Rockford, IL 61105
USA
(815) 968-0747
www.thermo.com/pierce
PRODUCT DATA SHEET
Analysis
Specification
UV Absorbance
Results
A280 = 1.0 -/+ 0.1
Pass
LC-MS chromatogram conforms to
reference
Ratio of peptide area to reference =
0.75-1.25
LC-MS Chromatogram
Reference Peptide Area
Pass
Pass
*Peptide Missed Cleavage
Tryptic peptide missed cleavage < 10%
Pass
*Peptide Alkylation
Cysteine carbamidomethyl
modification > 98%
Pass
*Peptide Oxidation
Methionine oxidation < 10%
Pass
*Other Peptide Modification
Carbamylation < 10%
Pass
* Peptide missed cleavage, alkylation, oxidation and modification determined by Preview™ Software
(Protein Metrics) using a human protein Swiss-Prot database.
RT: 0 .0 0 - 1 2 0 .0 0
NL:
1 .5 9 E 8
B a se P e a k m /z=
1 0 0 .0 0 -2 0 0 0 .0 0
F: ms MS
H e L a _ OJ 1 9 4 0 5 6
_ te s t2 _ 1 0 1 8 2 0 1 3
5 8 .6 0
100
90
5 5 .1 1
80
2 9 .9 5
Relative Abundance
70
60
3 0 .6 7 4 7 .6 1 4 9 .6 0
2 4 .3 9
7 4 .5 0
3 7 .3 7
50
6 1 .9 6
1 8 .9 3
4 3 .8 4
40
6 .0 2
1 8 .1 7
8 7 .8 5
6 4 .5 6
30
6 .5 7
1 7 .5 9
20
7 8 .8 0
9 4 .6 8
8 2 .7 4
9 7 .8 6
9 8 .7 3
10
1 0 4 .2 3
0
0
10
20
30
40
50
60
Tim e (m i n)
70
80
90
10 0
110
120
Base peak chromatogram of 200ng Pierce HeLa Protein Digest Standard separated using a
Thermo Scientific™ Acclaim™ PepMap100 3µm x 75µm x 15cm column (Product No. 160321) with a
2-35% gradient (A:0.1% FA in water, B: 0.1% FA in 100% acetonitrile) at 300nL/min for 120 minutes and
detected on a Thermo Scientific™ LTQ Orbitrap™ XL Mass Spectrometer.
Publish Date: 03/11/2014
Mike Stanaitis, Quality Chemist II
Products are warranted to operate or perform substantially in conformance with published Product specifications in effect at the time of sale, as set forth in the Product documentation, specifications and/or accompanying
package inserts (“Documentation”). No claim of suitability for use in applications regulated by FDA is made. The warranty provided herein is valid only when used by properly trained individuals. Unless otherwise stated in the
Documentation, this warranty is limited to one year from date of shipment when the Product is subjected to normal, proper and intended usage. This warranty does not extend to anyone other than Buyer. Any model or sample
furnished to Buyer is merely illustrative of the general type and quality of goods and does not represent that any Product will conform to such model or sample.
NO OTHER WARRANTIES, EXPRESS OR IMPLIED, ARE GRANTED, INCLUDING WITHOUT LIMITATION, IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR ANY PARTICULAR PURPOSE, OR NON
INFRINGEMENT. BUYER’S EXCLUSIVE REMEDY FOR NON-CONFORMING PRODUCTS DURING THE WARRANTY PERIOD IS LIMITED TO REPAIR, REPLACEMENT OF OR REFUND FOR THE NON-CONFORMING
PRODUCT(S) AT SELLER’S SOLE OPTION. THERE IS NO OBLIGATION TO REPAIR, REPLACE OR REFUND FOR PRODUCTS AS THE RESULT OF (I) ACCIDENT, DISASTER OR EVENT OF FORCE MAJEURE, (II)
MISUSE, FAULT OR NEGLIGENCE OF OR BY BUYER, (III) USE OF THE PRODUCTS IN A MANNER FOR WHICH THEY WERE NOT DESIGNED, OR (IV) IMPROPER STORAGE AND HANDLING OF THE PRODUCTS.
Unless otherwise expressly stated on the Product or in the documentation accompanying the Product, the Product is intended for research only and is not to be used for any other purpose, including without limitation,
unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses, or any type of consumption by or application to humans or animals. Preview is a trademark of Protein Metrics. TMT is a trademark
of Proteome Sciences plc. All other trademarks are the property of Thermo Scientific Inc. and its subsidiaries.
.
Pierce Biotechnology
PO Box 117
(800) 874-3723
(815) 968-7316 fax
3747 N. Meridian Road
Rockford, IL 61105
USA
(815) 968-0747
www.thermo.com/pierce