Geo-Mar Lett (2015) 35:69–75 DOI 10.1007/s00367-014-0391-1 SHORT COMMUNICATION The new CutSprof sampling tool and method for micromorphological and microfacies analyses of subsurface salt marsh sediments, Algarve, Portugal João Araújo-Gomes & Ana Ramos-Pereira Received: 25 September 2014 / Accepted: 3 November 2014 / Published online: 12 November 2014 # Springer-Verlag Berlin Heidelberg 2014 Abstract A new tool and method for collecting undisturbed subsurface samples in estuarine environments by means of trenching, timbering and sectioning is presented. Smoothing of sidewalls is achieved by a so-called cutting sediment profiler (CutSprof), while water draining into the trench is cleared by pumping. From smoothed sidewall sections, undisturbed thin sediment slices can then be collected for micromorphological and microfacies analyses. Results demonstrating the successful application of this procedure are presented for salt marshes of the Bensafrim River estuary (Lagos, Algarve, Portugal). In addition to palaeo-reconstructions in salt marsh settings, the CutSprof would be highly suitable in various other research domains as well as for environmental management purposes, particularly where sampling below the groundwater table is desirable to explore, for example, animal–sediment relationships in tidal-flat and mangrove ecosystems as well as the dynamics of coastal wetlands today threatened by ever-increasing anthropogenic influence. Introduction Important palaeoenviromental information critical in reconstructing environmental changes over time is commonly preserved in estuarine and salt marsh deposits (e.g. Hindson and Andrade 1999; Allen 2000; Boski et al. 2002; Heap et al. 2004; Vis et al. 2008; Kolditz et al. 2012; Kim et al. 2013). In addition, micro- and macrofossils may contribute to reconstruct relative sea-level histories at high precision (e.g. J. Araújo-Gomes (*) : A. Ramos-Pereira IGOT, Centre of Geographical Studies, University of Lisbon, 1600214 Lisbon, Portugal e-mail: [email protected] Franceschini et al. 2005; Woodroffe and Long 2009). Intertidal and supratidal estuarine sedimentary successions are particularly useful in this context because they record both sea-level fluctuations and changes which may have occurred in upstream terrestrial drainage basins (e.g. Ramos-Pereira et al. 2011). Traditional sampling methods in estuarine environments usually make use of augering and other coring techniques which also allow sampling below the water table (Lanesky et al. 1979; Rapp and Hill 1998). Although these procedures enable the extraction of long cores from water-saturated sandy and muddy sediments, cores from muddy substrates such as salt marsh deposits do not generally allow a full analysis of sedimentary and post-depositional alteration processes or undisturbed synsedimentary structures (Bullock et al. 1985), even if coring is performed with an Eijkelkamp piston sampler. This is because it is notoriously difficult to prepare epoxy resin peels from muddy substrates. This short paper focuses on presenting a new trenching procedure and cutting device particularly useful in the recovery of undisturbed subsurface samples for micromorphological and microfacies analyses of salt marsh deposits. The method was applied in the Bensafrim River estuary near Lagos, Algarve, Portugal, within the framework of the multidisciplinary estuarine research program FMI 5000 (RamosPereira et al. 2011), one main objective being the search for tsunami deposits and associated deformations. Such seismically deformed sedimentary records have previously been identified and analysed in a similar estuarine environment near Lagos (Hindson and Andrade 1999; Kortekaas and Dawson 2007; Cunha et al. 2009), but without the additional benefits of the technique described in this paper. An ultimate goal is to validate thin section analyses for the purpose of designing a diagnostic tool for the identification of local tsunami deposits sensu Kilfeather et al. (2007) and Bruins et al. (2008). 70 Brief outline of micromorphological approach Micromorphological investigations are rooted in micropedology (Kubiëna 1938; Stoops 2009). They are today a standard technique in soil studies and have received particularly strong attention in geoarchaeology over the last few decades (Cremaschi 2004). This geoscientific approach is an independent research field for the study of undisturbed soils, stratifications and regoliths (Bullock et al. 1985; Stoops 2003). In some cases, it has even been applied to obtain data on soil formation in space and time, factors essential in Fig. 1 a Map of Portugal and location of the study area. b Elevation model of the Bensafrim River basin showing the lithological boundary between the upper and lower catchment, as well as the coring and Geo-Mar Lett (2015) 35:69–75 understanding palaeoenvironmental conditions (cf. Goldberg 1979; McCarthy et al. 1998; Goldberg et al. 2003; Macphail et al. 2013). Micromorphology is a widely used approach in many types of research and in different environmental contexts (e.g. van der Meer 1993, 1997; Harris 1998; Kemp 1999; Goldberg and Byrd 1999; Carr 2001; Theler 2004; Stephens et al. 2005; Stolt and Lindbo 2010; Kilfeather et al. 2010; van der Meer and Menzies 2011). Excellent results have been achieved in coastal research (e.g. Macphail et al. 2010), hydropedology (e.g. Kutílek and Nielsen 2007) and archaeology (e.g. trenching sites (M marina). c Simplified log of the core recovered in 2013 (extracted from Araújo-Gomes 2013) Geo-Mar Lett (2015) 35:69–75 Macphail 2012). In spite of this, there are very few published papers specifically dealing with sampling methods and strategies for micromorphological research in general (cf. Goldberg and Macphail 2003), let alone for salt marsh environments. In practice, micromorphological investigations always begin with the extraction of blocks of undisturbed sediment along a vertical stratigraphic profile, which are then impregnated with an epoxy resin, polished and ground into thin sections for microscopic inspection (e.g. Bouma 1968; Courty et al. 1989). This procedure preserves sedimentary structures and is thus fundamental to environmental interpretation. Being undisturbed and precisely oriented in space, the epoxy peels and thin sections allow compositional and orientational analyses of the sample material (van der Meer and Menzies 2011). Physical setting The study was carried out in the estuarine section of the Bensafrim River along the southwest coast of Portugal (Fig. 1). At present, this estuary is a restricted mouth-bar estuary (cf. Cameron and Pritchard 1963; Dyer 1997) which has recently been transformed into a marina. The western margin is higher than the eastern one, being a Miocene limestone spur which forms the Ponta da Piedade. The eastern margin, located in a more rearward position, is formed by a fossil cliff carved into a sedimentary formation of probable Pliocene age, which is essentially composed of incoherent red sandstone (Ramos-Pereira 1990; Ramos-Pereira et al. 1994). Previous coring performed in the salt marsh upstream of the marina (Araújo-Gomes 2013) indicates that this estuary had once been an open estuary. 71 The drainage basin of the Bensafrim River can be subdivided into two distinct geological formations: (1) Palaeozoic turbidites, essentially composed of schists and greywackes, which occupy the upper half of the basin; and (2) carbonate and detrital formations, mainly composed of dolomitic limestones, sandstones (“Grés de Silves”; RamosPereira 1990) and marls forming the lower half of the basin (Fig. 1b). Earlier core analyses by Araújo-Gomes (2013) showed different sedimentary successions related to variable energy conditions, the upper units being the less energetic (Fig. 1c). From bottom to top the sedimentary succession comprises: (1) a >63-cm-thick silty sand bed; (2) a 47-cm-thick sandy clay bed; (3) a 51-cm-thick clayey silt bed with thin layers of fine sand intercalated; and (4) a 30-cm-thick surficial silty sand bed (sediment classification after Flemming 2000). In general terms it was concluded that (1) the silts and clays were probably derived from upstream turbidite deposits, whereas (2) the “Grés de Silves” (Silves Sandstone) is the main source of the sandy terrigenous sediments in the estuary; (3) based on AMS dating, the change from an open estuary to a bar-built one occurred at about 3 ka (Fig. 1c). Materials and methods As trenching was to be carried out to a depth below the water table, the application of a particular technique and procedure was required to avoid the collapse of trench sidewalls while allowing the recovery of undisturbed sediment samples (cf. Rapp and Hill 1998, p. 192). In the present case the study was confronted with a number of major issues: (1) insufficient information on the micromorphological and microfacies evolution of the estuarine infill; (2) the known existence of Fig. 2 Schematic diagrams of a trench (a), the design of the CutSprof cutting tool (b), and the sampling procedure (c) 72 seismically deformed sediments elsewhere in the Algarve (Schneider et al. 2009); and (3) the lack of a technical solution to overcome the problem of unstable trench sidewalls in water-logged sediments, which is probably the reason for the dearth of micromorphological and microfacies information in the first place. On the other hand, for simply describing the vertical stratigraphic sequence of the salt marsh deposits, exhaustive profile sampling was not required (e.g. Bullock et al. 1985; Courty et al. 1989). With these issues in mind, a suitable technique for the recovery of undisturbed subsurface samples in water-logged sediments was developed. The procedure and technique is schematically illustrated in Fig. 2. At first, a trench of suitable dimensions (length, width, depth) is excavated (Fig. 2a). To avoid the trench from flooding, it is drained by means of a submersible dirt-water pump powered by a portable generator. Fig. 3 a Trenching and timbering procedure before water pumping: strict safety measures are advised—always wear a helmet, firm shoes, and a rope around the waist in case of partial bogging. Adjustment and smoothing of trench sidewalls by means of CutSprof: b before cutting, c during cutting, d after cutting. Note the smooth vertical cut revealing undisturbed sediments Geo-Mar Lett (2015) 35:69–75 The collapse of the trench is prevented by supporting the sidewalls with wooden (or metal) boards held in place by adjustable steel struts (timbering procedure), but leaving narrow wall sections non-timbered for the purpose of sampling (Fig. 2a). Once the sidewalls have been stabilized and percolating water is under control, the trench can be safely entered. In a next step, the non-timbered sidewalls are vertically smoothed by means of a so-called CutSprof tool, a cutting sediment profiler specially designed for this purpose (Fig. 2b). It comprises a thin, spade-like, concave-shaped steel cutting edge supported by two or three vertical steel arms ending in a horizontal round steel or wooden handle. This cutting tool proved to be essential for producing perfectly smooth vertical surfaces required for the extraction of undeformed sediment samples. Spades and trowels were found to be inferior for this Geo-Mar Lett (2015) 35:69–75 purpose. After smoothing, thin soil slices can be removed by means of suitably sized plastic boxes and a normal spatula (Fig. 2c). After recovery, the boxes are sealed to avoid desiccation. In the laboratory, the samples can be X-rayed, impregnated with epoxy resin and ground into thin sections for microscopic analysis. Results In the case of the Bensafrim River estuary, the sidewalls of trenches excavated into the salt marsh are prone to collapse due to groundwater seepage from water-logged sediment layers. Figure 3a shows an excavated trench supported by steel boards held in place by an adjustable steel strut. The non-timbered sidewalls were then cut into vertical smooth surfaces by means of the CutSprof cutting tool as illustrated in Fig. 3b–d. For the recovery of undisturbed samples, simple plastic food boxes with clamp lids were used as sampling devices (Fig. 4a). The plastic is hard enough to endure extraction and transportation but soft enough to be pierced for later drying procedures. Although this type of container is rarely used because it is less resistant than other plastic boxes, they are relatively cheap, easily obtained in any supermarket, and their clamp lids provide perfect seals optimal for the preservation of sedimentary structures and water content. As shown in Fig. 4b, the thin section produced from such a box sample is well suited for micromorphological and microfacies analyses under a microscope. Fig. 4 a Sampling with a plastic food container box; b corresponding thin section 73 Discussion and conclusions A new procedure and particularly a tool were developed for this study because the poor stability of excavated trenches in water-saturated salt marsh deposits of the Bensafrim River estuary prohibited the use of standard techniques. Indeed, van der Meer and Menzies (2011) encouraged the adaptation of the micromorphological approach and techniques to suit the needs of a wider range of environments, providing specific examples where this had already been successfully achieved (e.g. Borchert 1968; Kooistra 1978; Johnson 1982; Kilfeather et al. 2007). In the present case, the sidewalls of the trenches were stabilized by adapting a form of timbering commonly used in street excavations. This involved the lining of the trench sidewalls with steel boards which were then held in position by an adjustable steel strut wedged between them. Seepage water collecting in the trench was drained by means of a submersible dirt-water pump. To obtain smooth vertical trench sections, a specially designed cutting tool, the CutSprof, was constructed. This new tool makes use of a concave cutting blade which is especially effective in ductile and watersaturated soils. It allows the adjustment and smoothing of vertical trench profiles, the pendulum shape and concave base facilitating lateral tilting motions to penetrate resistant or compacted soils. In addition to palaeo-reconstructions of, for example, tsunamis in salt marsh settings, the CutSprof would be highly suitable in various other research domains as well as for environmental management purposes. These include animal–sediment relationships in tidal-flat and mangrove ecosystems and the dynamics of coastal wetlands in general, 74 particularly where sampling below the groundwater table is desirable and also where anthropogenic impacts today pose ever-increasing challenges in coastal restoration (e.g. Perillo et al. 2005; Araújo et al. 2012; Lo et al. 2014). In conclusion, the findings convincingly demonstrate that the tools and the procedure developed for this work enable the recovery of undisturbed subsurface samples from excavated trench sidewalls even in water-saturated salt marsh environments below the groundwater table. The mechanical trenching, water pumping and profile timbering applied in the present study greatly reduce the risk of injury commonly associated with trenching. Acknowledgements The authors wish to thank the Portuguese Science and Technology Foundation (FCT-MCTES) for the financial support of this ongoing investigation within the research project PTDC/CTEGIX/ 104035/2008 - FMI 5000: Environmental changes: Fluvio-marine interactions over the last 5000 yrs. The assistance of the SLIF (Littoral and Fluvial Systems) team of the Centre for Geographical Studies, IGOT, University of Lisbon is also gratefully acknowledged. 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