Quarterly of the Horizon of Medical Sciences
Vol. 18, No. 4, Win 2013
Pages: 149-153
Effect of Angelica archangelica root hydro-alcoholic extract
on the blood cells of small laboratory mice
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Modaresi M.* PhD
‫٭‬Department of Animal Sciences, Faculty of Agriculture, Khorasgan Branch, Islamic Azad University, Isfahan, Iran
Abstract
Aims: Angelica archangelica is one of the medicinal herbs in traditional medicine which is
effective in hematopoiesis. In the present study, the effect of interperitoneal injection of
Angelica archangelica root’s hydroalcoholic extract in different doses on the count of red
and white blood cells, change in hemoglobin and hematocrit, mean globular volume, the
average size of hemoglobin and the hemoglobin average weight ratio in the Balb/c mouse,
were studied.
Methods: In this experimental study, 40 mature Balb/c male mice were divided into
control, placebo (with saline injection) and 3 treatment groups (injected with 50, 100 and
200mg/kg doses of Angelica archangelica root’s hydroalcoholic extract every other day for
20 days). Indices of red and white blood cells’ count, hematocrit and hemoglobin mean,
mean corpuscular volume, hemoglobin average volume and hemoglobin average weight
ratio were measured for each group separately. The results obtained were evaluated using
SPSS 11.5 software and Duncan's one-way ANOVA test.
Results: The average number of white blood cells in the first, second and third treatment
groups was significantly higher than control group (p<0.05). The average number of white
blood cells in the third group (200mg/kg) was significantly higher than the other groups
(p<0.05). There was no significant difference between the mean of hematocrit, hemoglobin,
MCV, MCH and MCHC of the groups (p>0.05).
Conclusion: The extract of Angelica archangelica is effective in hematopoiesis process
with the increase in the number of red blood cells and also with the increase in the number
of white blood cells in the immune system support.
Keywords: Angelica archangelica, Blood, Mice, Hemoglobins
*
Correspanding Author: All requests Should be sent to [email protected]
Received: 23 Dec 2010
Accepted: 9 Jul 2012
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10- Modaresi M, Gholchobian H. Effect of hydroalcoholic
extract of nettle on immune system in mice. Asian J
Chemistry. 2012;24(5):2339-41.
11- Modaresi M. A comparative analysis of the effects of
garlic, elderberry and black seed extract on the immune
system in mice. J Ani Vet Adv. 2012;11(4):458-61.
12- Marco K, Hui William K, Vivian Y, Wallace H.
Polysaccharide from the root of Angelica sinensis protects
bone marrow and gastrointestinal tissues against the
cytotoxicity of cyclophosphamide in mice. Int J Med Sci.
2006;3(1):1-6.
13- Cheng X, Chen J, Zhang P. Angelica stimulates
proliferation of murine bone marrow mononuclear cells by
the MAPK pathway. Blood Cells Mol Dis. 2006;36(3):4025.
14- Sarker SD, Nahar L. Natural medicine: The genus
Angelica. Curr Med Chem. 2004;11(11):1479-500.
15- Wang Y, Zhu B. The effect of angelica polysaccharide
on proliferation and differentiation of hematopoietic
progenitor cell. Zhonghua Yi Xue Za Zhi. 1996;76(5):363-6.
16- Hatano R, Takano F, Fushia S, Michimata M, Tanaka T,
Kazama I, et al. Water-soluble extracts from Angelica
acutiloba Kitagawa enhance hematopoiesis by activating
immature erythroid cells in mice with 5-flurouracil-induced
anemia. Exp Hematol. 2004;32(10):918-24.
17- Wang PP, Zhang Y, Dai LQ, Wang KP. Effects of
Angelica sinensis polysaccharide-iron complex on iron
deficiency anemia in rats. Chin J Integr Med.
2007;13(4):297-300.
18- Chang MS, Kim do R, Ko EB, Choi BJ, Park SY, Kang
SA, et al. Treatment with Astragali radix and Angelicae
radix enhances erythropoietin gene expression in the
cyclophosphamide-induced anemic rat. J Med Food.
2009;12(3):637-42.
19- Maliakal PP, Wanwimolruk S. Effect of herbal teas on
hepatic drug metabolizing enzymes in rats. J Pharm
Pharmacol. 2001;53(10):1323-9.
Q Horizon Med Sci
Vol. 18, No. 4, Win 2013
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1- Braun L, Cohen M. Herb and supplement: An evidencebased guide. 4th ed. New York: Elsevier Mosby Publishers;
2005.
2- Mir Haidar H. Plants used in the prevention and treatment
of disease. 6th ed. Tehran: Islamic Culture Publication; 2005.
[Persian]
3- Flok H. Medicinal plants. 5th ed. Tehran: Golshan
Publication; 2000. [Persian]
4- Zargari A. Medicinal plants. Tehran: Tehran University
Publication; 1985. [Persian]
5- Yang M, Chan GC, Deng R, Cheng SW, Lau CP, Wang
L, et al. An herbal decoction of Radix astragalia and Radix
angelica
Sinensies
promotes
hematopoiesis
and
thrombopoiesis. J Ethnopharmacology. 2009;124(1):87-97.
6- Lee S, Shin DS, Kim JS, Kang SS. Antibacterial cumarins
from Angelica gigas roots. Arch Pharmacol Res Seol.
2003;26(6):449-52.
7- Jaroslaw W, Milena P, Konstantia G, Kazimierz G,
Loanna Ch. Coumarins from Angelica Lucida Antibacterial
activities. Molecules. 2009;14(8):2729-34.
8- Thomson Ml. Immunomodulatory effects of traditional
Chinese herbal formulation, ginseng and dong qui ten
combination (PS10). Swinbure; University of Technology;
2006.
9- Lopes D, Strobl H, Kolodziejczyk P. 14Methylpentadecano-15-lactone
(muscolide):
A
new
macrocyclic lactone from the oil of Angelica archangelica.
Chemist Biodivers. 2004;1(12):1880-7.
Downloaded from hms.gmu.ac.ir at 6:56 +0430 on Sunday June 18th 2017
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