Cell Cycle and Cell Division by CHED on June 18, 2017 lesson duration of 10 minutes under General Biology 1 generated on June 18, 2017 at 02:26 am Tags: The Cell CHED.GOV.PH K-12 Teacher's Resource Community Generated: Jun 18,2017 10:26 AM Cell Cycle and Cell Division ( 1 hour and 10 mins ) Written By: CHED on July 21, 2016 Subjects: General Biology 1 Tags: The Cell Resources Biology 10th Ed Mader,Sylvia S.2011.Biology 10th Ed.Mac Graw Hill Education, USA The World of the Cell Becker, Wayne M, Lewis J. Kleinsmith, and Jeff hardin. 2000, The World of the Cell, Addison Wesley Longman,Inc. USA Campbell Biology, 10th Ed Reece, Jane B. et al, 2013. Campbell Biology, 10th Ed. Pearson Education, Inc. United States of America. Biology 6th Ed. Raven, Peter and George Johnson 2001. Biology 6th Ed.The McGraw Hill Company,USA Genes in Motion: Mitosis https://www.genome.gov/pages/education/dnaday/newsandfeatures/genesinmotionlessonguide.pdf Biological Significance of Mitosis & Meiosis in Sexual Reproduction http://education.seattlepi.com/biological-significance-mitosis-meiosis-sexual-reproduction-5259.html Gametogenesis http://csls-text.c.u-tokyo.ac.jp/active/12_05.html Meiosis: function/stages http://www.vcbio.science.ru.nl/en/virtuallessons/meiostage/ Meiosis with Crossing Over http://highered.mheducation.com/sites/9834092339/student_view0/chapter11/meiosis_with_crossing_over.html 1/9 CHED.GOV.PH K-12 Teacher's Resource Community Cell Division, Mitosis, and Meiosis http://bio100.class.uic.edu/lecturesf04am/lect16.htm Three Activities for Teaching Cell Cycles http://www.brighthubeducation.com/middle-school-science-lessons/94267-three-activities-for-teaching-cell-cycles/ Content Standard The learners demonstrate an understanding of: 1. Cell Theory 2. Cell Structure and Functions 3. Prokaryotic vs Eukaryotic Cells 4. Cell Types 5. Cell Modifications 6. Cell Cycle a. Mitosis b. Meiosis 7. Transport Mechanisms a. Simple Diffusion b. Facilitated Transport c. Active Transport d. Bulk/Vesicular Transport Performance Standard The learners shall be able to: 1. construct a 3D model of a plant/animal/ bacterial cell using recyclable materials 2. construct a cell membrane model from indigenous or recyclable materials Learning Competencies The learners characterize the phases of the cell cycle and their control points The learners describe the stages of mitosis/meiosis given 2n=6 2/9 CHED.GOV.PH K-12 Teacher's Resource Community The learners discuss crossing over and recombination in meiosis The learners explain the significance or applications of mitosis/meiosis The learners identify disorders and diseases that result from the malfunction of the cell during the cell cycle Introduction 5 mins 1. Introduce a simplified life cycle of a human being or a plant. Let the students identify the changes and how these organisms grew and developed. Courtesy of Dr. J. Derksen; drawings Lidwien van der Horst http://www.vcbio.science.ru.nl/en/virtuallessons/c... Motivation 5 mins a) Before discussion play the video entitled “Cell “Cell Cycle and Cell Division”. Division”. This video can be downloaded from you tube or open the hyperlink below if with ready internet connection. http:// www..youtube. http://www youtube.com/ com/watch? watch?v=Q6ucKWIIFmg or b) Show diagrams of cell division in multicellular/eukaryotic organisms Instruction/Delivery 30 mins Lecture-Discussion (using powerpoint presentation/diagram in a manila paper/video ) or demonstrate the process using model materials (like: beads, cords/yarn with varied thickness, coins etc or summary of the answers of the learners after asking questions related to the video. 3/9 CHED.GOV.PH K-12 Teacher's Resource Community Lesson 1 (STEM_BIO11/12-Id-f-6) – characterization of the phases of the cell cycle and their control points Lesson 2. (STEM_BIO11/12-Id-f-7) - description of the stages of mitosis and meiosis (given 2n=6 ) (A summary Table can be introduced after the discussion: http://courses.washington.edu/bot113/spring/WebReadings/PdfReadings/TABLE_COMPARING_MITOSIS_AND.pdf) http://courses.washington.edu/bot113/spring/WebReadings/PdfReadings/TABLE_COMPARING_MITOSIS_AND.pdf) Stages of plant and animal mitosis 4/9 CHED.GOV.PH K-12 Teacher's Resource Community Stages of plant and animal meiosis Mitosis Compared with Meiosis Meiosis Mitosis 1. Requires two nuclear divisions 1. Requires one nuclear division 2. Chromosomes synapse and cross 2. Chromosomes do not synapse nor cross over over 3. Centromeres survive Anaphase I 3. Centromeres dissolve in mitotic anaphase 4. Halves chromosome number 4. Preserves chromosome number 5. Produces four daughter nuclei 5. Produces two daughter nuclei 6. Produces daughter cells 6. Produces daughter cells genetically genetically different from parent and each other 7. Used only for sexual reproduction Meiosis I compared to Mitosis Meiosis I Mitosis Prophase I Prophase identical to parent and to each other 7. Used for asexual reproduction and growth Meiosis II compared to Mitosis Meiosis II Mitosis Prophase II Prophase 5/9 CHED.GOV.PH K-12 Teacher's Resource Community Pairing of homologous No pairing of chromosomes chromosomes Metaphase I Metaphase Bivalents at metaphase plate Duplicated chromosomes at metaphase plate No pairing of chromosomes No pairing of chromosomes Metaphase II Metaphase Haploid number of duplicated Diploid number of duplicated chromosomes at metaphase chromosomes at metaphase plate plate Anaphase I Anaphase Anaphase II Anaphase Homologues of each bivalent Sister chromatids separate, Sister chromatids separate, Sister chromatids separate separate and duplicated becoming daughter becoming daughter becoming daughter chromosomes move to poles chromosomes that move to chromosomes that move to chromosomes that move to the poles the poles the poles Telophase I Telophase Telophase II Telophase Two haploid daughter cells Two diploid daughter cells, Four haploid daughter cells Two diploid daughter cells, not identical to the parent cell identical to the parent cell not genetically identical identical to the parent cell Lesson 3. (STEM_BIO11/12-Id-f-8) - discussion of crossing over and recombination in meiosis https://www.google.com.ph/search?q=crossing+over+a... Lesson 4. (STEM_BIO11/12-Id-f-9) - explanation of the significance or applications of mitosis/meiosis Lesson 5. (STEM_BIO11/12-Id-f-10) - identification of disorders and diseases that result from the malfunction of the cell during the cell cycle 6/9 CHED.GOV.PH K-12 Teacher's Resource Community Practice 10 mins 1. Provide the class with games like Amazing Race or Interphase/Mitosis/Meiosis Puzzle This activity follows a series of stations (stages/phases of events with challenges they have to accomplish. Divide the class into (depending on the number of stages/phases in the activity, like for interphase/mitosis/meiosis) groups after the discussion and introduce the process. These groups will race to accomplish the following tasks in five stations. Each station will assemble given materials to illustrate stages or phases of events. (depending on the lesson/topic like for example interphase/mitosis/meiosis. 2. Provide the students a Crossword Puzzle 7/9 CHED.GOV.PH K-12 Teacher's Resource Community Enrichment 20 mins Students may be directed to watch some more you tube videos on cell division. A number of good videos have the stages/phases made into a rap or a song. One such example is the video entitled Cell Division Song Spongebob that could be viewed in the link below: http:// www..youtube. http://www youtube.com/ com/watch? watch?v=9nsRufogdoI =9nsRufogdoI Introduce animal and plant gametogenesis to further appreciate the significance of cell division. (http://csls-text.c.u-tokyo.ac.jp/active/12_05.html) http://csls-text.c.u-tokyo.ac.jp/active/12_05.html) Microscopic examination of onion root tip. (http://w3.marietta.edu/~biol/introlab/Onion (http://w3.marietta.edu/~biol/introlab/Onion root...f) root...f) Procedure for preparing root tip squashes. 1.Using scissors, cut 2 roots tips about 1 cm long, and transfer them into a plastic micro-tube. (One of the rots will be an extra one.) 2. Fill the tube about 2/3 full with 1N HCl from a dropper bottle. *** Caution: Work with the HCl carefully, it is a strong acid. *** 3. Place the tube in a 60 C water bath, and allow the roots to incubate for 12 minutes. 4. After the 12 minute incubation period, remove the tube from the water bath. 5. Rinse the roots in H2O. 6. Using forceps, carefully transfer the root tips to a small petri plate. 7. Using a plastic ‘squeeze’ pipet, carefully remove the HCl from the micro-tube and transfer it to the “discard flask”. 8. Rinse the root tips 3 times with water from the dropper bottle, disposing of the rinses in the discard flask. 9. After removing the water from the third rinse, cover the root with the Feulgen stain. *** Caution: Although the Feulgen stain does not appear colored, it will strongly stain skin and clothing. *** 10. Incubate the roots in the stain for 12 minutes. During this time the very tip of the root will begin to turn red as the DNA stains the numerous small actively dividing cells at the tip. 11. Using a plastic ‘squeeze’ pipet, carefully remove the Feulgen stain and discard it in the discard flask. 12. Again, rinse the root tips 3 times with water. 8/9 CHED.GOV.PH K-12 Teacher's Resource Community 13. Transfer a root to the center of a clean microscope slide and add a drop of water. 14. Using a razor blade cut off most of the unstained part of the root, and discard it. 15. Cover the root tip with a cover slip, and then carefully push down on the cover slide with the wooden end of a dissecting probe. Push hard, but do not twist or push the cover slide sideways 16. The root tip should spread out to a diameter about 0.5 – 1 cm. 17. Examine under the microscope and record your observations. 5 0 mins Dance Performance using the concepts involved in cell Division. e.g Each group will perform their version of mitosis/meiosis with their fellow classmates. Each performance should take no longer than 5-7 minutes and can be accompanied by music. Students should see several different ways in which mitosis/meiosis can be depicted with each carrying the same steps through out. The performances should help clarify misconceptions that students may have and help bring their understanding of mitosis/meiosis to a higher level. Rubrics will be used to assess the performance. Download Teaching Guide Book 0 mins 9/9 Powered Poweredby byTCPDF TCPDF(www.tcpdf.org) (www.tcpdf.org)
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