Biomedical Sciences at CMCA
NEW FACILITIES AND APPLICATIONS
CMCA@Perkins – complete move from M Block in January 2015
400 sqm state-of-the-art purpose built PC2 core
laboratories featuring;
 Small animal bioimaging
• High field small animal in vivo MRI (9.4 Tesla)
• High resolution in vivo micro-CT (up to 9 µm
resolution)
• In vivo multispectral imaging (fluorescence,
luminescence)
 Cytometry
• High resolution, high throughput, polychromatic
flow cytometry analysers (up to 18 colours)
• High speed, aseptic cell sorting (4 way sorts, 18
colours, single cell sorts)
 Microscopy
• Confocal, multiphoton and epifluorescent
microscopes
• Immunohistochemistry & histology digital slide
scanners
The University of Western Australia
Third Floor
The University of Western Australia
Biomedical Applications Group
Academic-led
 A/Prof Kirk Feindel (Bioimaging)
([email protected])
 A/Prof Matt Linden (Cytometry)
([email protected])
 A/Prof Paul Rigby (Microscopy)
([email protected])
 Prof David Sampson (Biomed Engineering)
([email protected])
 A/Prof Andrew Mehnert (Data Viz / Informatics)
(coming soon!)
Collaborations
Internal review of NHMRC grant applications
The University of Western Australia
Installed 2014:
State-of-the-art electron microscopy facilities
 First of their kind in Australia
 FEI Titan ChemiSTEM
• Atomic resolution imaging
• Nanoscale element mapping
• Electron tomography
 Funded through ARC LIEF (Partners:
UWA, CUT, MU, ECU, CSIRO)
 FEI Verios XHR SEM
• Sub-nm resolution imaging
• Sub-μm resolution element mapping
 Funded through ARC LIEF (Partners:
UWA, MU, ECU, Kings Park)
Contact Prof Martin Saunders ([email protected])
The University of Western Australia
Coming in 2015
 Mass Cytometry (Successful ARC LIEF)
• Cytometry by atomic mass spectroscopy time of flight
(CyTOF)
• Highly multi-parametric cell-by-cell characterisation
• Simultaneous analysis of immune sub-phenotype,
function and signalling
The University of Western Australia
The University of Western Australia
Arriving 2015 Thermo Fusion Mass Spectrometer (Successful ARC LIEF Grant)
• Mass Analysis of intact Proteins with
ETD and top down protein analysis.
• Accurate Protein Quantitation
• Simple, reliable ETD enhances
characterization of complex PTMs
• Determine structures more quickly and
accurately with resolution of isobaric
interferences.
• MSn with up to 400,000 resolution
Contact Dr Anthony Reeder ([email protected])
The University of Western Australia
Automated digital fluorescence slide scanning
(BHP CCWA Grant application)
• Multiplexed with spectral unmixing
• Deep phenotyping of cancer and immune
cells in situ
• Objective / automated image analysis
The University of Western Australia
Grant planning 2015…
 Optical Super-resolution Microscopy
(LIEF Grant Application 2015)
• SIM – structured illumination
microscopy
– Approx 2x improvement in
resolution (100nm)
– 3D and live cells
• STORM – locates fluorophores
using “position probability”
– Approx 10x improvement in
resolution (10nm)
– Fixed cells only
Contact A/Prof Paul Rigby
([email protected])
Microtubules
&
Microtubules
Microtubules
Clathrin
(confocal)
(STORM)
5µm
5µm
200nm
The University of Western Australia