Data file 29-1151-92 AB
High- and super-resolution imaging
DeltaVision™ Elite Microscopy System
DeltaVision Elite is an integrated high resolution fluorescence
microscopy platform designed for general fluorescence
imaging applications. Multiple refinements of the optical
system in combination with advanced automation optimize
the system for low light imaging of live cells. The DeltaVision
Elite system supports several options for advanced imaging
modalities and is an excellent platform for a range of
fluorescent imaging needs.
Microscope and objectives
Microscope base
The DeltaVision Elite system is based on one of the most
stable and successful inverted microscope stands on the
market, the Olympus® IX-71. Together with our advanced
stage and optics modules, this provides the flexibility to
support a range of imaging modalities.
Table 1. Microscope base properties
Fig 1. The DeltaVision Elite Microscopy system.
Objective lenses
Microscope base
Customized Olympus IX-71 inverted microscope
Safety features
Additional safety interlock controls installed for
laser options
Objective
6 position objective nosepiece
PLAPON60×O, 1.42 NA, WD 0.15 mm included
The Olympus IX-71 microscope at the heart of the DeltaVision
Elite system supports a wide range of high-class microspcope
objectives. Table 2 lists a selection of objectives that are most
suited to the applications for which DeltaVision is optimized.
Eyepiece
10× eyepieces, with diopter adjustment
Eyepiece filter wheel added
Stages
Focus range
9 mm, manual
Standard Flexure stage
Light path
100% eyepiece or 100% camera selectable
Filters
6 position filter cube holder
Optional motorized cube holder available
(Code number: 53-852648-000)
Control
Custom circuitboard and electronics
Operating
conditions
Indoor use
Ambient temperature 18°C to 25°C (65°F to 77°F)
Incorporating GE’s proprietary Flexure design and
Nanomover™ technologies, the DeltaVision Elite stage is an
exceptionally accurate, reliable, and operationally reproducible
design. The stage can reliably return to the same field of view
with repeatability within the diffraction limit of the microscope
optics. This enables accurate continuous monitoring of
multiple fields of view over extended periods of time.
NA = numerical aperture. WD = working distance
gelifesciences.com
Table 2. Selection of objectives suitable for use with DeltaVision Elite system
Code number
Magnification
Optical
corrections
NA
WD
(mm)
Immersion
34-100642-000
4×
Super-Plan APO
34-100641-000
10×
Super-Plan APO
34-100640-000
20×
34-018019-121
20×
34-100639-000
DIC
Correction Notes
ring
0.16
13
n.a.
No
Microtiter plate or tissue imaging
0.40
3.1
n.a.

No
Microtiter plate or tissue imaging
Plan Fluor
0.45
6.6–7.8
n.a.

Yes
Microtiter plate or tissue imaging
Super-Plan APO
0.75
0.6
n.a.

No
Microtiter plate or tissue imaging
40×
Plan Fluor
0.60
2.7–4
n.a.

Yes
Microtiter plate or tissue imaging
53-850009-122
40×/Oil
Plan Fluor
1.30
0.2
Oil

No
PSF certified objective
General imaging
52-852175-116
60×/Oil
Plan APO
1.42
0.15
Oil

No
PSF certified objective
Primary objective lens supplied
with DeltaVision Elite system
52-852956-000
60×/Silicone
Plan APO
1.3
0.30
Silicone oil

Yes
Live cell imaging
53-850009-114
60×/Water
Super-Plan APO
1.2
0.28
Water

No
PSF certified objective
Live cell imaging
34-018019-120
60×/TIRF
APO
1.49
0.10
Oil

Yes
TIRF Imaging,
Localization Microscopy
53-850009-111
100×/Oil
Super-Plan APO
1.4
0.13
Oil

No
PSF certified objective
General imaging
34-018019-119
100×/TIRF
APO
1.49
0.1
Oil

Yes
TIRF Imaging,
Localization Microscopy
Plan = Flat field correction, DIC = differential interference contrast, APO = Apochromat, indicating color correction, Super-Plan = Flat field correction over larger field of view,
PSF = point spread function, Fluor = Fluorite, n.a. = not applicable
Microtiter plate stage
Table 3. Standard Flexure stage specifications
x- and y-axes
z-axis
Accuracy
< 10.0 µm per 25 mm
< 0.6 µm per 13 µm
Repeatability range
≤ 0.4 µm
≤ 0.2 µm
Step resolution
10 nm
10 nm
Maximum travel
25 mm (x) × 50 mm (y)
1 mm
Speed
xy-axes 2 mm/s, z-axis 1 mm/s
Automation control
3D multisite visiting within one sample or
multiple wells depending on sample holder
Supported sample
types
75 × 25 mm slides, multichambered
coverslips/slides, 35 mm and 40 mm dishes
Code number
52-851244-001
The microtiter plate stage incorporates advanced Nanomover
technology, and allows the use of standard multi-well
microtiter plates for higher throughput imaging applications.
Table 4. Microtiter plate stage specifications
x- and y-axes
z-axis
Accuracy
< 10.0 µm per 25 mm
< 0.6 µm per 13 µm
Repeatability range
≤ 0.4 µm
≤ 0.2 µm
Step resolution
10 nm
10 nm
Maximum travel
106 mm (x) × 70 mm (y) 1 mm
Speed
xy-axes 3 mm/s, z-axis 1 mm/s
Automation control
3D multisite visiting within well(s)
Supported sample
types
SBS style microtiter plates (6, 12, 24, 96 and
384 wells). Using supplied sample holder
insert, 75 × 25 mm slides, 35 mm and
40 mm dishes
Code number
52-851879-001
Fig 2. Standard Flexure stage.
Fig 3. Microtiter plate stage.
2 29-1151-92 AB
Enclosure and isolation platforms
Table 6. Environmental chamber ordering information
Instrument enclosures
Code number
Description
Isolating the sample from the surrounding environment
considerably improves system stability. System enclosures
that isolate the instrument from fluctuations in room
temperature are highly recommended for long term
time-lapse imaging (over hours or days) or high accuracy
applications such as Total Internal Reflection Fluorescence
(TIRF) or Localization Microscopy imaging.
53-851569-012
Environmental Control Chamber, Transparent,
120 V for DeltaVision Elite with Flexure stage
53-851569-112
Environmental Control Chamber, Transparent,
240 V for DeltaVision Elite with Flexure stage
53-851569-013
Environmental Control Chamber, Opaque, 120 V
for DeltaVision Elite with Flexure stage
53-851569-113
Environmental Control Chamber, Opaque, 240 V
for DeltaVision Elite with Flexure stage
53-851861-012
Environmental Control Chamber, Transparent,
120 V for DeltaVision Elite with microtiter stage
53-851861-112
Environmental Control Chamber, Transparent,
240 V for DeltaVision Elite with microtiter stage
53-851861-013
Environmental Control Chamber, Opaque, 120 V
for DeltaVision Elite with microtiter stage
53-851861-113
Environmental Control Chamber, Opaque, 240 V
for DeltaVision Elite with microtiter stage
Enclosures for the DeltaVision Elite system are available
in clear or opaque configurations. Opaque enclosures are
particularly useful in environments where ambient light
conditions may interfere with imaging.
Isolation platforms and tables
The DeltaVision Elite system is available with a choice of
isolation systems to suit both space and application needs.
• The benchtop configuration utilizes a high performance
isolator platform with minimal footprint to support the
microscope. The CPU and IC/MIC modules can be placed
where convenient for the user.
Fig 4. Enclosures for DeltaVision Elite system may be either clear or opaque.
This composite illustration shows a clear enclosure as the left half and an
opaque as the right.
Table 5. DeltaVision Elite enclosure specifications
• The standard table features the same high performance
isolators from the isolation platform integrated into
a heavy duty table for additional stability. Electronic
components are mounted in a matching cabinet.
• The advanced isolation system utilizes the highest
performance bearings giving maximum isolation for
applications such as TIRF or localization microscopy.
Electronic components are mounted in a matching cabinet.
Options
Transparent or Opaque
Air flow
User selectable speed
Temperature control
ambient + 7°C to 45°C ± 1°C
Temperature stability
± 0.2°C
Ambient temperature
18°C to 25°C
Table 7. Isolation platform ordering information
Chamber dimensions
89 × 51 × 33 cm (35 × 20 × 13 in)
Code number
Description
Supply voltage
100–120 V or 220–240 V
52-851263-001
Benchtop isolation platform with isolators
for vibration control. No additional cabinet is
provided for electronic components
53-853262-100
Benchtop isolation platform isolators
integrated into a high density table support
and accompanying rack-mount cabinet for
electronics.
53-853262-200
High density table support with advanced
isolation and accompanying rack-mount cabinet
for electronics.
29-1151-92 AB 3
Focus systems
Illumination
UltimateFocus hardware autofocus module
Brightfield illumination system
The DeltaVision UltimateFocus system incorporates an
exclusive patented design to automatically maintain the
sample z-position regardless of mechanical or thermal
changes. The system provides real-time compensation of
stage drift and focus control within 50 nm. UltimateFocus
also enables Focus Assist, which allows the user to bring the
objective into the area of focus without using the eyepieces
or camera, by utilizing the autofocus system’s sensor. This
minimizes unnecessary illumination of the sample and
reduces photobleaching.
Brightfield imaging with DeltaVision Elite uses a specially
designed transilluminator arm assembly, incorporating a
long-life white light LED. An optional brightfield condenser
allows differential interference contrast imaging and other
transmitted light modes.
A)
A)
B)
B)
Fig 6. (A) Specially designed transilluminator arm. (B) Optional brightfield
condenser.
Table 9. Brightfield illumination system specifications
Fig 5. UltimateFocus hardware autofocus module. (A) Part of the larger
Trulight assembly and (B) close up view.
Table 8. UltimateFocus hardware autofocus model specifications
Focus precision
50 nm (with 60× 1.42 NA oil lens)
Response time
200 ms
Laser wavelength
785 nm
Cover glass
No. 1.5, (nominal thickness 170 µm)
Item
Details
GE-designed
transillumination
arm
White light LED, rated 100 Lumens at 350 mA
Estimated lifetime 50 000 hours at 700 mA
Condenser mounting bracket
Brightfield
condenser unit
(optional)
Universal Long Working Distance (LWD)
condenser
Numerical aperture 0.55 NA
Working distance 27 mm
5 position wheel, (3 × 30 mm, 2 × 38 mm)
DIC components
(optional)
Shift type DIC slider
Lens specific DIC prisms 20× 40×, 60×, 100×
available
Condenser and filter wheel polarizers
Note: Focus precision is dependent on objective lens used.
Note: DIC prisms can be purchased in conjunction with a brightfield condenser or added
to an existing condenser.
Table 10. Brightfield illumination system ordering information
4 29-1151-92 AB
Code number
Description
53-850989-000
Universal long working distance (LWD) condenser
assembly with DIC prism hold ring. Includes
Condenser, DIC Prism Shift Slider and Polarizer.
DIC prisms for specific objective lenses need to
be purchased separately.
34-018019-135
DIC Nomarski Prism for LWD condenser. Matched
for 20×/0.75 objective lens (34-018019-121)
34-018019-132
DIC Nomarski Prism for LWD condenser. Matched
for 40×/1.3 objective lens (53-850009-122)
34-018019-133
DIC Nomarski Prism for LWD condenser. Matched
for 60×/1.42 objective lens (52-852175-116)
34-018019-134
DIC Nomarski Prism for LWD condenser. Matched
for 100×/1.4 objective lens (53-850009-111)
Trulight fluorescent illumination module
Table 11. InsightSSI general specifications
The TruLight illumination system delivers more light to the
sample with outstanding signal-to-noise performance,
enabling detection of small, dim objects such as organelles
and microbial particles. Seamless automation handles
switching between sample viewing (Köhler illumination)
and image acquisition (optimized critical illumination). The
TruLight module also supports a range of optional hardware
to extend system performance, including UltimateFocus,
Multi-line TIRF, PhotoKinetic (PK) and DLM modules.
On time 0% to 100%
< 150 µs
Off time 100% to 0%
< 8 µs
Wavelength switching time
< 1 ms
Optical fiber
1 mm diameter, 0.39 NA
Operational lifetime
> 5000 h
Table 12. Four-color standard set InsightSSI module and filter configurations
(Code number: 52-852113-003)
Channel
Band (nm)
Center
wavelength
(nm)
Bandwidth
(nm)
Single band pass excitation filters
DAPI
381–399
390
18
FITC/GFP
461–489
475
28
528.5–555.5
542
27
621–643
632
22
TRITC/Cy™3
Fig 7. TruLight fluorescent illumination module.
Cy5/Alexa Fluor™ 647
Single band pass emission filters
Fluorescent illumination sources
DAPI
411–459
435
48
DeltaVision Elite uses a proprietary solid state illumination
system, the InsightSSI, that provides extremely stable and
long-lasting illumination with electronic control for rapid on/
off operation. Switching between wavelengths takes less than
1 ms, making this an ideal illumination source for multicolor
imaging, particularly for fast dynamics. Light is delivered
to the sample through a 1 mm fiber that gives uniform
illumination across the specimen. The InsightSSI illumination
system does not generate wavelengths of light below 350 nm
which can be damaging to live cells.
FITC/GFP
501–549
525
48
TRITC/Cy3
574.5–619.5
597
45
662–696
679
34
A)
B)
Cy5/Alexa Fluor 647
Polychroic beam splitter (suitable for DAPI, FITC, TRITC, Cy5)
DAPI EX
381–412
396.5
31
DAPI EM
420–456
438
36
FITC EX
464–492
478
28
FITC EM
500–523
511.5
23
TRITC EX
531–565
548
34
TRITC EM
573–611
592
38
Cy5 EX
619–646
632.5
27
Cy5 EM
654–700
677
46
EM = emission, EX = excitation
Fig 8. InSightSSI fluorescence illumination module. (A) Front view and
(B) rear view.
29-1151-92 AB 5
Table 13. Seven-color combined set InsightSSI module and filter
configurations (Code number: 52-852113-024)
Channel
Band (nm)
Table 14. Four-color fluorescent protein (Live Cell) InsightSSI Module
(Code number: 52-852113-013)
Center
wavelength Bandwidth
(nm)
(nm)
Single band pass excitation filters
DAPI
CFP
FITC/GFP
YFP
TRITC/Cy3
Alexa Fluor 594/mCherry
Cy5/Alexa Fluor 647
381–399
426–450
461–489
504.5–521.5
528.5–555.5
562.5–587.5
621–643
411–459
463–487
501–549
537–559
574.5–619.5
602.5–647.5
662–696
18
24
28
17
27
25
22
435
475
525
548
597
625
679
48
24
48
22
45
45
34
Polychroic beam splitter suitable for DAPI, FITC, TRITC, Cy5
DAPI EX
DAPI EM
FITC EX
FITC EM
TRITC EX
TRITC EM
Cy5 EX
Cy5 EM
381–412
420–456
464–492
500–523
531–565
573–611
619–646
654–700
396.5
438
478
511.5
548
592
632.5
677
31
36
28
23
34
38
27
46
Polychroic beam splitter suitable for DAPI, FITC/GFP,
mCherry/Alexa Fluor 594, Cy5
DAPI EX
DAPI EM
FITC EX
FITC EM
mCherry EX
mCherry EM
Cy5 EX
Cy5 EM
381–412
420–456
464–492
500–549
557–590
598–617
625–646
654–700
396.5
438
478
524.5
573.5
607.5
635.5
677
31
36
28
49
33
19
21
46
Polychroic beam splitter suitable for CFP, YFP, mCherry
CFP EX
CFP EM
YFP EX
YFP EM
mCherry EX
mCherry EM
6 29-1151-92 AB
400–453
463–487
497–527
537–549
557–592
602–662
Band (nm)
Single band pass excitation filters
390
438
475
513
542
575
632
Single band pass emission filters
DAPI
CFP
FITC/GFP
YFP
TRITC
Alexa Fluor 594/mCherry
Cy5/Alexa Fluor 647
Channel
Center
wavelength Bandwidth
(nm)
(nm)
426.5
475
512
543
574.5
632
53
24
30
12
35
60
CFP
FITC/GFP
YFP
Alexa Fluor 594/mCherry
426–450
461–489
504.5–521.5
562.5–587.5
438
475
513
575
24
28
17
25
475
525
548
625
24
48
22
45
Single band pass emission filters
CFP
FITC/GFP
YFP
Alexa Fluor 594/mCherry
463–487
501–549
537–559
602.5–647.5
Polychroic beam splitter suitable for CFP, YFP, mCherry
CFP EX
CFP EM
YFP EX
YFP EM
mCherry EX
mCherry EM
400–453
463–487
497–527
537–549
557–592
602–662
426.5
475
512
543
574.5
632
53
24
30
12
35
60
Polychroic beam splitter suitable for DAPI, FITC/GFP,
mCherry/Alexa Fluor 594, Cy5
DAPI EX
DAPI EM
FITC/GFP EX
FITC/GFP EM
mCherry EX
mCherry EM
Cy5 EX
Cy5 EM
381–412
420–456
464–492
500–549
557–590
598–617
625–646
654–700
396.5
438
478
524.5
573.5
607.5
635.5
677
31
36
28
49
33
19
21
46
Table 15. Power ratings for InsightSSI fluorescent illumination sources
Power (mW)
Channel
DAPI
CFP
FITC/GFP
YFP
TRITC
Alexa Fluor 594/mCherry
Cy5/Alexa Fluor 647
Min.
Max.
55
90
50
16
90
70
50
95
120
75
28
125
100
80
Filter wheels
High performance filter wheels give flexibility for a wide variety
of applications. With standard size filters that are simple
to change, the system can easily be adapted for custom
fluorophores and new dye choices. Switching time between
adjacent positions on the wheels is 100 ms for 10 position filter
wheels and 250 ms for 6 position filter wheels.
Table 16. Filter wheel configurations. Actual filter positions may vary depending on system configuration
and user preference
Item
Details
4 color standard dye SSI
filter wheel
6-position emission filter wheel including four EM filters,
1 polarizer, 1 open position
Position
Example dye
1
DAPI 2
FITC/GFP 3
TRITC 4
Cy5/Alexa Fluor 647
5
Polarizer for DIC
0Open
4 color fluorescent protein
SSI filter wheel
6-position emission filter wheel including four EM filters,
1 polarizer, 1 open position
Position
Example dye 1
CFP
2
FITC/GFP
3
YFP
4
mCherry/Alexa Fluor 594
5
Polarizer for DIC
0Open
7 color SSI filter wheel
Wavelengths
475/24 nm
525/48 nm
548/22 nm
625/45 nm
10-position emission filter wheel including 7 emission filters,
1 polarizer and 2 open positions
Position
Example dye
1Open
2
CFP 3
YFP 4
Alexa Fluor 594/mCherry
5
FITC/GFP
6
DAPI
7
TRITC
8
Cy5/Alexa Fluor 647
9
Polarizer for DIC
0Open
Eyepiece filter wheel
Wavelengths
435/48 nm
525/48 nm
597/45 nm
679/34 nm
Wavelengths
475/24 nm
548/22 nm
625/45 nm
525/48 nm
435/48 nm
597/45 nm
679/34 nm
7-position eyepiece filter wheel including 6 EM filters,
excluding Cy5 emission filter and 1 polarizer
Position
1
2
3
4
5
6
0
Example dye
DAPI TRITC
FITC/GFP mCherry/Alexa Fluor 594
CFP
YFP
Polarizer for DIC
Wavelengths
435/48 nm
597/45 nm
525/48 nm
625/45 nm
475/24 nm
548/22 nm
Note: Filters are positioned to group the live cell set together and the fixed cell set together, with the shared FITC/GFP
filter between them. This minimizes the distance moved during typical experiments, which is important since filter
switching speed can be a limiting factor in performance.
29-1151-92 AB 7
DeltaVision Elite TIRF/PK Module
DeltaVision Localization Microscopy option
TIRF is a specialized imaging modality, particularly useful
for cell surface microscopy, single molecule localization,
and membrane research. The modality is critical to superresolution techniques such as DeltaVision Localization
Microscopy, where the high-contrast, low-noise illumination
configuration is ideal for detection of single molecules.
DeltaVision Localization Microscopy System is an advanced
imaging option for the DeltaVision Elite and DeltaVision OMX
platforms. It employs total internal reflection fluorescence
(TIRF) imaging in combination with laser activation control
to acquire frames of fluorophore images. These images are
analyzed with our exclusive Dense Stochastic Sampling
Imaging (DSSI) algorithm, which uses multiple-Gaussian
fitting to find the locations of fluorophores within a sample;
the fluorophore locations are used to reconstruct a superresolution image.
The DeltaVision TIRF module also supports PK applications
through the addition of optional PK optics. This allows noninvasive manipulation of live cell samples by either bleaching
or activating selected critical molecules with a high intensity,
focused light pulse. The PK spot size is approximately
1.5 µm with a 60× objective and 1 µm with 100× objective.
Precise spot size depends on the wavelength and the optical
characteristics of the sample.
PK capability forms the basis of such techniques
such as Photoactivation (PA), Fluorescent Recovery
After Photobleaching (FRAP), and Fluorescent Loss in
Photobleaching (FLIP).
Flexible design enables PK applications (PK-GFP) to be
combined with TIRF imaging.
• Provides 2-D resolution between 20 and 50 nm (subject
to appropriate sample preparation, labeling density, and
localization precision)
• Compatible with photoactivation, photoconversion, and
photoswitching fluorophore systems
• DSSI algorithm can resolve overlapping signals from
fluorophores spaced closer than the diffraction limit
in dense fields, thereby enabling localization at higher
densities
Table 18. DeltaVision Localization Microscopy specifications
Fig 9. DeltaVision Elite TIRF/PK module.
Code number
29089302
(For newly configured systems only)
2D localization precision
~ 20 nm. Localization precision is
dependent on the number of photons
collected
2D lateral resolution
< 50 nm. Requires appropriate sample
preparation and labeling density
Achievable under ideal conditions
Fluorophore system
Photoactivation, photoconversion,
photoswitching
Lasers supported
405 nm, 100 mW
488 nm, 100 mW
568 nm, 150 mW
640 nm, 100 mW
Table 17. DeltaVision Elite TIRF/PK module ordering information
Code number
Description
Laser intensity control
Direct laser power control
52-852305-001
TIRF optics module only
Laser shuttering
~ 5 ms fast electronic shutters
Activation laser control
Pulse duration and laser intensity
adjustable during acquisition
Cameras supported
CoolSNAP™ HQ2 CCD,
Evolve™ EMCCD or pco.edge sCMOS
Chromatically corrected TIRF module
automatically adjusts each laser line to the same
TIRF penetration depth, eliminating the need to
adjust each laser line independently
Flexible design enables the combination of
photokinetic applications (PA-GFP) with TIRF
imaging
52-852306-001
Photokinetics optics module only
PK Spot size:
~ 1.5 µm with 60× objective
~ 1 µm with 100× objective
Actual bleach spot size is dependent on
wavelength and optical characteristics of
sample.
52-852307-001
8 29-1151-92 AB
Combined TIRF and PK optics module
Objective lenses
CoolSNAP HQ2
pco.edge sCMOS
Evolve EMCCD
60× objective
60× objective
60×, 100× objectives
Acquisition pixel size
CoolSNAP HQ2
pco.edge sCMOS
Evolve EMCCD
Evolve EMCCD
108 nm at 60× objective
108 nm at 60× objective
133 nm at 60× + 2× Optivar
80 nm at 100× + 2× Optivar
Standard X4 module lasers
The X4 laser module is an integrated unit supporting up to
4 lasers for a variety of applications including TIRF, PK and
Localization microscopy imaging.
• A choice of eight lasers, six wavelengths, for TIRF and
PK applications allows greater flexibility in the use of
fluorescent dyes and proteins
• Higher power laser options for Localization Microscopy
applications at 488 nm and 568 nm wavelengths
• Rapid laser switching enables combinations of multiple
lasers to photoactivate and photobleach the sample
within the same experiment
Table 19. X4 laser chassis ordering information
Code number
Description
53-852719-101
X4 laser chassis, no lasers
Table 20. X4 laser module specifications
Laser
405 nm
445 nm
488 nm
514 nm
568 nm
640 nm
Code number
29092657
53-852473-001
53-852720-001
(50 mW)
53-852105-001
29128881
(50 mW)
53-852471-001
29092659
(150 mW)
29092658
(100 mW)
Type
Diode
Diode
OPSL
OPSL
OPSL
Diode
Wavelength (nm)
403 ± 2
445 ± 5
488 ± 2
514 ± 2
568 ± 2
640 ± 5
Rated power output (mW)
100 mW
40 mW
50 or 100 mW
50 mW
50 or 150 mW
100 mW
Nominal power out from X4
module
~ 22 mW
~ 8 mW
~ 23 mW
for 50 mW
~ 18 mW
~ 18 mW
for 50 mW
~ 39 mW
~ 54 mW
for 150 mW
~ 45 mW
for 100 mW
Spatial mode
TEM∞
TEM∞
TEM∞
TEM∞
TEM∞
TEM∞
Beam diameter (mm, 1/e )
0.8–0.9 mm
1.1 mm
0.7 ± 0.05 mm
0.7 ± 0.05 mm
0.7 ± 0.05 mm
1.0 mm
Beam divergence (mrad)
~ 0.5
~ 0.5
< 1.2
< 1.3
< 1.3
< 1.2
M² (typical)
< 1.25
< 1.3
< 1.1
< 1.1
< 1.1
< 1.2
Beam circularity
1
0.9–1.1
0.9–1.1
0.9–1.1
0.9–1.1
–
Pointing stability (μrad/⁰C)
<5
<6
< 10
< 10
< 10
<6
Power stability (over 24 hours)
< 2%
< 2%
< 0.6%
< 0.6%
< 0.6%
< 2%
Polarization orientation
Vertical ± 2⁰
Vertical
Vertical
Vertical
Vertical
Vertical ± 5⁰
Polarization extinction ratio
> 100:1
> 100:1
> 100:1
> 100:1
> 100:1
> 100:1
RMS noise (10 Hz to 10 MHz)
0.1%
0.1%
< 0.25%
< 0.2%
< 0.2%
0.1%
Input power (head)
12 VDC
4.8–6.5 VDC
10.8–15 VDC
10.8–15 VDC
10.8–15 VDC
4.8–6.5 VDC
CDRH class
Class IIIb
Class IIIb
Class IIIb
Class IIIb
Class IIIb
Class IIIb
ESD protection
Class 4
Class 4
Class 4
Class 4
Class 4
Class 4
EU compliance
CE Mark Certified CE Mark Certified CE Mark Certified CE Mark Certified CE Mark Certified CE Mark Certified
with control box
with control box
RoHS compliance
EU and China
2
EU and China
EU and China
EU and China
EU and China
EU and China
29-1151-92 AB 9
Cameras
Camera systems
Part of the flexibility of the DeltaVision Elite microscope
platform is the range of supported cameras, addressing the
needs of different applications.
Table 21. Supported camera specifications
CoolSNAP HQ2
Standard
CoolSNAP ES2
Budget conscious
pco.edge sCMOS
High performance
Evolve EMCCD
High sensitivity (optional
secondary camera)
Image sensor
CCD
CCD
Scientific CMOS
EMCCD
Chip size
1392 × 1040 pi×els
1392 × 1040 pi×els
2560 × 2160 pi×els
512 × 512 pi×els
Sensor size (imaging area)
8.98 × 6.71 mm
8.98 × 6.71 mm
16.6 × 14 mm
8.2 × 8.2 mm
Pi×el size
6.45 × 6.45 µm
6.45 × 6.45 µm
6.5 × 6.5 µm
16 × 16 µm
Read out speeds
10 and 20 MHz
10 and 20 MHz
95 and 286 MHz
1 MHz, 5 MHz, 10 MHz
Max. operational speed*
13.4 fps at 512 × 512
8.2 fps at 1024 × 1024
13.4 fps at 512 × 512
8.2 fps at 1024 × 1024
400 fps at 512 × 512
200 fps at 1024 × 1024
20.4 fps at 512 × 512 (EMCCD
Mode only at 10 MHz readout)
Cooling
-30°C air-cooled Peltier
0°C air-cooled
+5°C stabilized Peltier cooling -80°C air cooled Peltier
Dynamic range
12 bit (0–4095 counts)
12 bit (0–4095 counts)
15 bit (0–32767 counts)
16 bit (0–65355 counts)
Spectral range
320–1020 nm
320–1020 nm
370–1100 nm
300–1050 nm
Quantum efficiency
~ 60% at peak (540 nm)
~ 60% at peak (540 nm)
~ 54% at peak (540 nm)
87% at peak (540 nm)
Full well capacity
16 000 e-
13 500 e-
30 000 e-
200 000 e-
Read noise
4.5 e- at 10 MHz,
5.5 e- at 20 MHz
8 e- at 20 MHz
1.4 e-
10 MHz EM Port: 45 e5 MHz EM Port: 32 e5 MHz non-EM Port: 12 e1 MHz non-EM Port: 6 e-
Operating environment
0°C to 30°C ambient, 0%
to 80% relative humidity
non-condensing
15°C to 30°C ambient, 0%
to 80% relative humidity
non-condensing
10°C to 40°C ambient, 10%
to 80% relative humidity
non-condensing
0°C to 30°C ambient, 0%
to 80% relative humidity
non-condensing
* The operational speed of the camera system is dependent on the electronics control unit of the system. Values may vary depending on overall system configuration.
Control and data acquisition
DeltaVision Elite Workstations
The DeltaVision Elite is controlled by an optimized workstation
system in conjunction with the IC/MIC integrated controller.
Two different configurations are available depending on
the camera type chosen. The system runs on the CentOS
operating system, a variant of Linux. This is a highly stable
platform that is particularly suited for long term operation of
the computer and ideal for computationally demanding tasks
such as image reconstruction and deconvolution operations.
Additional workstations can be purchased for offline data
processing.
Note: Computer hardware specifications evolve rapidly so workstation system
specifications may change without notice.
10 29-1151-92 AB
Fig 10. DeltaVision system workstation.
Table 22. Standard workstation for CCD and EMCCD-equipped systems
DeltaVision SoftWoRx™ software
CPU
Single processor, Intel™ Core™ i7
CPU speed
3.4 GHz, 8 MB cache
RAM
16 GB, 1333 MHz
Primary hard disk
1 TB SATA
RAID array
3 × 1 TB RAID-5 Data Array on dedicated
controller
The DeltaVision Elite system is controlled using the SoftWoRx
software package. Acquisition and experimental control
operates through the Resolve3D module that provides highly
flexible experimental design and control, with excellent
stability for long term imaging.
Video card
NVIDIA™ Quadro™ NVS300 512 MB PCIE
Network interface
Dual Gigabit Ethernet
Optical drive
Integrated DVD-RW drive
Operating system
CentOS v6 or higher
Also included in SoftWoRx are a variety of visualization
and routine analysis tools for viewing acquired data and
performing quantitative analysis.
Available as options are SoftWoRx Explorer, a Windows
based file viewer and SoftWoRx Suite, a more advanced
visualization and analysis package which includes our
exclusive deconvolution algorithm for image reconstruction.
Table 23. High performance workstation for sCMOS-equipped systems
CPU
Single processor, Intel Core i7
CPU speed
3.4 GHz, 8 MB cache
RAM
16 GB, 1333 MHz
Primary hard disk
1 TB SATA
RAID array
3 × 1 TB RAID-5 Data Array on dedicated
controller
Fast cache array
2 × 120 GB SATA III Solid State Drive RAID-0
Fast Write
Video card
NVIDIA Quadro NVS300 512 MB PCIE
Network interface
Dual Gigabit Ethernet
High speed camera
network interface
40 Gigabit Infiniband
Optical drive
Integrated DVD-RW drive
Operating system
CentOS v6 ir higher
Fig 11. SoftWoRx explorer screenshot.
Table 24. Standard monitor supplied with the system
Model
Dell™ U2413 Widescreen Flat Panel
Display or equivalent
Panel size (diagonal)
24 in (61 cm)
Aspect ratio
Widescreen (16:10)
Panel type
IPS (In-plane switching)
Optimal resolution
1920 × 1200 at 60 Hz
Contrast ratio (typical)
1000 to 1
Dynamic contrast ratio
(maximum)
80 000:1
Brightness (typical)
350 cd/m2
Response time
(gray to gray)
6 ms
Maximum viewing angle 178° vertical / 178° horizontal
Pixel pitch
0.27 mm (94 pixels/in)
29-1151-92 AB 11
Table 25. SoftWoRx functionality
Item
Details
Software system
SoftWoRx v6 (or higher). Requires CentOS v5 (or higher)
Image acquisition and
operational control
Resolve3D (integrated with SoftWoRx on acquisition workstations)
Deconvolution
Deconvolution improves image contrast and resolution without sacrificing data integrity.
Exclusive deconvolution algorithm results in true quantitative data.
• Quantitative iterative constrained algorithm
• Increased contrast and resolution in x, y and z axes
• Deconvolve images on-the-fly to visualize data faster
Cell tracking
Cell tracking algorithms automatically reposition the stage to accurately follow cells as
they move during time-lapse experiments.
• Defines region of interest to track object within field of view
• Automatically tracks the stage to keep target cell(s) in the center of the field-of-view
• Uses collected data to calculate rates and direction of motion
Point visiting
Visit multiple points within a single or time-lapse experiment. This technology ensures
that the stage will return to the same location in x, y and z.
• Provides outstanding point-to-point accuracy and precision motion control
• Map and record hundreds of points to maximize data collection within an experiment
• Streams collected data to image processing tasks to improve workflow
Optical axis integration
OAI is an approach for rapidly acquiring data in the z axis based on user defined ranges. It
quickly captures and visualizes data that otherwise may be missed.
• Images moving diffraction-limited objects that may otherwise be lost between frames
• Lowers total light exposure, increasing cell viability
• Rapidly captures the total number of objects within a cell (e.g., centrosomes, viral particles)
General functionality
Exported image formats: JPG, TIFF
Exported video formats: AVI, Quicktime
Volume Rendering and 3D Visualization
Measuring tools: Line Profile, Polygon Analysis, Intensity Plots
Data Analysis and Export to spreadsheet via CSV file format
For local office contact information, visit
www.gelifesciences.com/contact
Products are for research purposes only. They are not approved for diagnosis of disease in humans or animals.
GE and GE monogram are trademarks of General Electric Company.
Cy, DeltaVision, Nanomover, and SoftWoRx are trademarks of General Electric Company or one of its subsidiaries.
www.gelifesciences.com/deltavision
Alexa Fluor is a trademark of Life Technologies Corporation. CoolSNAP and Evolve are trademarks of
Photometrics. Dell is a trademark of Dell Inc. Intel and Core are trademarks of Intel Corporation. NVIDIA and
Quadro are trademarks of NVIDIA Corporation. Olympus is a registered trademark of Olympus Corporation.
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© 2014 General Electric Company—All rights reserved.
First published Aug. 2014
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29-1151-92 AB 09/2014