A case of subfertile cow with structural abnormalities of
the X-chromosome
H. Hanada, S. Muramatsu
To cite this version:
H. Hanada, S. Muramatsu. A case of subfertile cow with structural abnormalities of the Xchromosome. Annales de génétique et de sélection animale, INRA Editions, 1980, 12 (2),
pp.209-213. <hal-00893216>
HAL Id: hal-00893216
https://hal.archives-ouvertes.fr/hal-00893216
Submitted on 1 Jan 1980
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Note
A
case
of subfertile cow with structural abnormalities
of the X-chromosome
H. HANADA
S. MURAMATSU
Animal Breeding and Genetics,
National Institute of Animal Industry,
Tsukuba Norindanchi, P.O. Box 5
, Ibaraki-ken PC 305
Department of
(Japan)
Summary
Cytogenetic examination was performed on a three years old Japanese Black, cow, which
had been slaughtered due to subfertility. Structural chromosome abnormalities were found
in qi (
20 p. 100
) out of 200 mitotic cells analysed.
The aberrations including deletions affected more specifically the X-chromosome. The
frequency was significantly higher than that observed normally in cultures from a cow population of the same breed.
cattle with either sub- or infertility have recently
In general, these animals showed a significantly high
incidence of structural abnormalities of the autosome and /or the X-chromosome
,et
I
F,
-N al., I
I, 1972
A
(H
,
NAN
g7q.; BONGSO and BASRUR, 1976). And,
; AHASS
the
found
that
Er.-Nnxess et al. 6)
frequency of chromosome abnormalities
197
(
was lower for A..r. bulls and breeding cows than for slaughter Cattle.
Similar
results were obtained in Swine population (Mlcx!!MANN et al., 1977
). In the
course of a chromosome survey of the Japanese Black Cattle, a case of low fertile
cow with an unusually high frequency of structural abnormalities of the X-chromosome was detected.
The animal for which the chromosome examination was performed was a
three years old Japanese Black cow, which had been culled owing to repeated
abortions. Blood sample was taken from the femoral vein before slaughter, and
cultured in vitro for 4
8 hrs accordira to the procedure described previously
Cytogenetic studies of
appeared in the literature.
A and MuRanzn2su, 19
D
(HarrA
). Two hundred well-spread metaphases were
0
8
selected under the microscope and studied on microphotographs.
No phenotypic abnormalities were detected in the animal. The results of
cytogenetic analysis are given in Table i. Most cells .5
) showed normal
79 p. 100
(
chromosome constitution without any structural abnormality. The rest (
41 cells;
.5 p. 100
20
) were abnormal cells with one or two structural abnormalities of the
autosome and
n =
2
lor
the
X-chromosome, but with
a
normal female
complement,
,XX.
0
6
The details of abnormalities are shown in Table 2
.
The autosomal abnormalities were found in 20 cells (ro.o p. 100
6 chromosomes, whereas
) involving 2
those on the X-chromosomes were observed in 22 cells .
) involving 2! X11 p. 100
(
chromosomes. The frequency and the nature of autosomal abnormalities were
similar to those occurring spontaneously in cultures from a cow population of the
same breed.
On the other hand, the incidence of abnormalities of the X-chromosomes was about 6 times higher than that observed spontaneously.
Furthermore, chromosome deletions, which were extremely rare in normal cattle, were
observed with a high incidence (
, P < ).
9
.
001 In 5 cells, as shown in
0
Z2= 47
figure i, aberrations were recognized on both X-chromosomes. In the present case
subfertility in a cow was associated with a significant number of abnormalities of
the X-chromosom es.
Similar results have been reported in a two years old infertile Black and White
heifer (!r,-NAHess et al., 1974
) and a three months old Holstein calf affected with
chronic tympanism of the rumen (G
URY 197
G
ST and ,
E
EN
8).
On the other hand, autosomal abnormalities have also been detected in a high
percentage of mitotic cells in cultures from sub- or infertile cattle (HAr,n.!N, 2;
97
r
Bowso and
ASRUR 197
B
,
6). These results suggest that there is a definite association between low fertility and a high incidence of structural abnormalities of
the X-chromosome and /or the autosome.
Received
for publication
in
August ig8o.
Résumé
Subfertilité
chez la vache
Une étude
accom!agnée
d’anomalies structurales d-u chromosome X
a été réalisée chez une vache subfertile appartenant à la race
avait été abattue à l’âge de trois ans. Des anomalies chromosomiques structuraux ont été révélées dans 41 parmi les 200 cellules mitotiques comptées ,
20 p. 100
(
5
).
Les anomalies, y compris des délétions, affectaient plus particulièrement le chromosome X.
La fréquence des anomalies était statistiquement plus élevée que celle normalement observée
chez les vaches de cette race.
cytogénétique
Noire Japonaise qui
Références
BorTGSO
ASRUR P. K., 197
A., B
6. Chromosome anomalies in Canadian Guernsey bulls. Cornell
Vet., 66, 47
88.
4
61!!,-N:!xnss )!., S
VRJALLA A., M1CHELMANN H. W., PAUFLER S., 1974
. Mosaik einer X-Chro-
mosomen-Aberration als wahrscheinliche LTrsache der Sterilitât bei einem Rind. Dtsch. tierârztl.
Wschv., 81, 39!-398.
h., b2icxEW tArr!r
Ei<-NAHASS
H. W., Peuxr,EU S., 197
6. Chromosomale Untersuchungen von
Zucht- und Schlachtrindern. Züchtungsk., 48, .
277
4
6
2
EST P., G
N
GE
UAV P., 197
8. Structural abnormalities of the X-chromosome in a heifer. Canad.
J. Com
. M!., 43,
p
iio-ni.
Hnr,rrnN C.R.E., 1972
. Autosomal deletion and infertility in cattle. Vet. Rec., 91, .
72
5
Hnr,rraN C.R.E., 6.
197 A cytogenetic survey of 1101 Australian cattle of 25 différent breeds.
Avcn. Gévcét. Sél. anim., 8, 131
.
9
-13
ADA H., TS
N
A
H
URAMA S., 19
M
U
. Spontaneous chvomosome aberration rate in the pevipheval
0
8
lymphocytes of the Japanese Black Cattle (to be submitted).
1
M
L
E
MANN
CH H. W., El.-N
AHASS lâ. M., P!uxr.ER S., 1977
. Vergleichende Chromosomenuntersuchung bei Zucht- und Mastschweinen mit Hilfe der Giemsa-Piirbung und der B!,nderungstechnik. Züchtmagsk., 49, 294
-300.