Phytofuse Rejuvenate®
Wound Healing + Anti-Inflammatory + Film-Forming
Tomorrow’s Vision… Today!®
Phytofuse Rejuvenate®
Technical Information:
Product Code: 16882
INCI Name: Salvia Hispanica Seed Extract
INCI Status: Conforms
Suggested Use Level: 1.0 - 10.0%
Suggested Applications: Wound Healing, Anti-Inflammatory, Film Forming,
Moisturizing, Soothing, Antioxidant
Phytofuse Rejuvenate®
Background
•
Salvia hispanica, commonly known as chia, is a desert plant
native to central and southern Mexico and Guatemala
•
Produces several small white and black seeds with a remarkable
nutritional profile that surpasses that of any other superfood
•
Hydrophilic properties of the Salvia hispanica seed produces a
unique protective mucilage
• Allows the seed to retain enough water to germinate and
grow in semi-arid regions
•
The protective mucilage is rich in polysaccharides with the
potential for innovative cosmetic and personal care applications
Phytofuse Rejuvenate®
Background
•
“Chia” is the ancient Mayan word for “strength”
•
Skin loses strength and elasticity over time,
resulting in fine lines and visible signs of aging
•
Phytofuse Rejuvenate® harnesses the therapeutic
properties of Salvia hispanica seed mucilage to
naturally strengthen and revitalize the skin
•
The Mayans and Aztecs used this now wildly
popular superfood in regenerative medicine as a
poultice for gunshot wounds to promote healing
and reduce inflammation
•
Restorative qualities of the seeds are a result of
mucilaginous polysaccharide
Phytofuse Rejuvenate®
Polysaccharides
•
Polysaccharides are long chain carbohydrates capable of stabilizing biomolecules and
assemblages, such as cell wall membranes
•
Polysaccharides, such as hyaluronic acid, are sought after for their unparalleled film
forming and hydration capacities in cosmetics and personal care formulations
•
Additionally, they contribute to the skin’s natural ability for repair and renewal
•
High molecular weight polysaccharides, such as β-Glucans, have potent
immunomodulatory effects that regulate and improve the efficiency of the immune system
• Exhibit anti-inflammatory effects on the skin
Phytofuse Rejuvenate®
Skin Repair & Renewal
•
Skin is constantly exposed to micro-wounds and abrasion
• Exposure to the elements (cold weather, wind, UV radiation)
• Drying surfactants
• Shaving
•
This exposure results in a compromised barrier function
• Dry, inflamed skin
•
Phytofuse Rejuvenate®
• Isolated polysaccharides from the Salvia hispanica seed mucilage are biotransformed into high molecular weight polysaccharides via fermentation
• Capable of wound healing and anti-inflammatory benefits
Phytofuse Rejuvenate®
Benefits
 Anti-inflammatory
 Pro-Collagen Synthesis
 Wound Healing
 Improves Barrier Function
 Natural Film Former
 Moisturizing
 Intense Antioxidant
 Anti-aging
Phytofuse Rejuvenate®
Protocol
IL-6 ELISA Assay
•
Human dermal fibroblasts seeded
into 12-well tissue culture plates
•
Phytofuse Rejuvenate® added to
complete DMEM containing
1µg/mL LPS and incubated with
fibroblasts for 24 hours
•
Concentrations: 1%, 0.1%, 0.01%
•
Decrease in IL-6 production
indicates a reduced inflammatory
environment which could decrease
the signs of aging and reduce the
formation of fine lines and wrinkles
2000
1800
IL-6 Concentration (pg/mL)
1600
1400
1200
1000
800
600
400
200
0
Complete Media
1μg/Ml lps
1μg/mL LPS + 10μM
DEX
1.0% Phytofuse
Rejuvenate®
Figure 1. Phytofuse Rejuvenate® treated fibroblasts IL-6 concentrations
Phytofuse Rejuvenate®
Scratch Assay
T = 72
T = 72
•
Human dermal fibroblasts were
seeded into 6-well tissue culture
plates
•
0.1% & 0.2% concentration of
Phytofuse Rejuvenate® was added
to the serum-free DMEM and
incubated with fibroblasts
•
Scratch took place every 24 hours
and up to 72 hours
•
Phytofuse Rejuvenate® was able to
increase cell migration and close the
scratch at a rate comparable to the
positive control
•
Product has healing abilities and cell
proliferation properties
0.1% Phytofuse
Rejuvenate®
Negative Control
Positive Control
T=0
Protocol
Figure 2. Images right after scratch, after 72 hours and after 72 hours but
stained for enhanced microscopy
Phytofuse Rejuvenate®
Protocol
Comparative Moisturization
140
Percent (%) Difference
120
•
Equipment: DermaLab Combo
•
Principle of measurement:
Conductance, single frequency
•
Subjects: 10 (m/f)
•
Test area: Volar forearms
•
Concentration used: 2.0%
•
Frequency of application: Twice daily
100
80
60
40
20
0
T = 24 Hours
T = 1 Week
T = 2 Weeks
T = 3 Weeks
T = 4 Weeks
2.0% Phytofuse Rejuvenate® in Base Lotion vs. Untreated
2.0% Phytofuse Rejuvenate® in Base Lotion vs. Base Lotion
Figure 3. Improvements in moisturization
Phytofuse Rejuvenate®
Protocol
Transepidermal Water Loss
45%
40%
Percent (%) Decrease
35%
•
Equipment: DermaLab Combo
•
Principle of measurement: Open
chamber, vapor diffusion gradient
•
Subjects: 10 (m/f)
•
Test area: Volar forearms
•
Concentration used: 2.0%
•
Frequency of application: Twice daily
•
Results indicate continuous
improvements in the barrier of the skin
throughout the 4 week test period
30%
25%
20%
15%
10%
5%
0%
T = 24 Hours
T = 1 Week
T = 2 Weeks
T = 3 Weeks
2.0% Phytofuse Rejuvenate® in Base Lotion vs. Untreated
2.0% Phytofuse Rejuvenate® in Base Lotion vs. Base Lotion
Figure 4. Improvements in barrier function
T = 4 Weeks
Phytofuse Rejuvenate®
Percent (%) Difference
Comparative Difference in Skin Density
Protocol
30%
•
Equipment: DermaLab Combo
25%
•
Subjects: 10 (m/f)
20%
•
Test area: Volar forearms
15%
•
Concentration used: 2.0%
•
Frequency of application: Twice daily
•
Phytofuse Rejuvenate® is capable of
improving skin density when compared
to both the untreated control as well
as the base lotion
10%
5%
0%
T = 24 Hours
T = 1 Week
T = 2 Weeks
T = 3 Weeks
T = 4 Weeks
2.0% Phytofuse Rejuvenate® in Base Lotion vs. Untreated
2.0% Phytofuse Rejuvenate® in Base Lotion vs. Base Lotion
Figure 5. Improvements in skin density
Phytofuse Rejuvenate®
Protocol
ORAC Assay
Antioxidant Capacity (μMTE)
250
•
Trolox® was used as the positive control
•
Solutions were prepared at three
concentrations, as a reference
•
Fluorescent measurements were taken
every 2 minutes for 2 hours
•
Phytofuse Rejuvenate® showed
antioxidant activity
200
150
100
50
0
200μM Trolox
12.5μM Trolox
5% Phytofuse
Rejuvenate®
2.5% Phytofuse 1.25% Phytofuse
Rejuvenate®
Rejuvenate®
Figure 6. Antioxidant capacity of Phytofuse Rejuvenate®
Phytofuse Rejuvenate®
Cellular Viability
Protocol
Relative Fluorescence Units (RFU)
90000
•
Human dermal fibroblasts seeded
into 96-well tissue culture plates
•
Ten microliters of viability reagent
was added to 90µL of cell culture
media in culture wells
•
Concentrations: 1%, 0.10%, 0.01%
•
Phytofuse Rejuvenate® at all
concentrations does not inhibit
cell viability and is not cytotoxic
85000
80000
75000
70000
65000
Complete Media
1% Phytofuse
Rejuvenate®
0.1% Phytofuse
Rejuvenate®
0.01% Phytofuse
Rejuvenate®
Figure 7. Cellular metabolism of Phytofuse Rejuvenate® treated
fibroblasts in terms of relative fluorescence units (RFU)
Phytofuse Rejuvenate®
Protocol
Sirius Red Fast Green Assay
70
•
AA2G and IGF-1 were used as
positive controls
•
Test Quantity: 1%, 0.1%, and
0.01%
•
200µL of the Sirius Red/Fast
Green dye solution added and
incubated at room temperature
for 30 minutes
•
Phytofuse Rejuvenate® elicited
positive effects on collagen
synthesis and may lead to
improvement in the dermalepidermal junction integrity and
improved scaffolding matrix
Protein Concentration (μg)
60
50
40
30
20
10
0
100μg/mL 50ng/mL IGF-1
Ascorbic Acid
Complete
Media
Collagen Proteins
1% Phytofuse
Rejuvenate®
0.1%
Phytofuse
Rejuvenate®
Non-Collagen Proteins
Figure 8. Collagen & non-collagen protein concentrations
0.01%
Phytofuse
Rejuvenate®
Phytofuse Rejuvenate®
Protocol
Sirius Red Fast Green Assay
% Collagen/Non-Collagen Proteins
6
•
AA2G and IGF-1 were used as
positive controls
•
Test Quantity: 1%, 0.1%, and
0.01%
•
200µL of the Sirius Red/Fast
Green dye solution added and
incubated at room temperature
for 30 minutes
•
Phytofuse Rejuvenate® elicited
positive effects on collagen
synthesis and may lead to
improvement in the dermalepidermal junction integrity and
improved scaffolding matrix
5
4
3
2
1
0
100μg/mL 50ng/mL IGF-1
Ascorbic Acid
Complete
Media
1% Phytofuse 0.1% Phytofuse
Rejuvenate® Rejuvenate®
Figure 9. Percent collagen compared to non-collagen proteins
0.01%
Phytofuse
Rejuvenate®
Phytofuse Rejuvenate®
Product Recap
•
Phytofuse Rejuvenate® capitalizes on the isolated polysaccharides extracted
from the Salvia hispanica seed
• Able to aid in cell proliferation
• Imparts wound healing and anti-inflammatory properties to the skin
•
Phytofuse Rejuvenate® soothes the skin and provides antioxidant and
moisturization benefits
• A functional, active ingredient
• Improves the slip and cushion in finished formulations
•
Phytofuse Rejuvenate® is ideal for skin and hair care formulations
• Helps to increase moisture levels and provide protection from
environmental stressors
Phytofuse Rejuvenate®
Technical Information:
Product Code: 16882
INCI Name: Salvia Hispanica Seed Extract
INCI Status: Conforms
Suggested Use Level: 1.0 - 10.0%
Suggested Applications: Wound Healing, Anti-Inflammatory, Film Forming,
Moisturizing, Soothing, Antioxidant
Active Concepts LLC
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