OVOIL
™
• 100% paraffin oil of highest quality.
• Extensively tested.
Embryo development
under different oil types
• Confirmed excellent embryo development.
Paraffin oil and mineral oil are chemically slightly
different. The polycarbon lipid tail of mineral oil
contains more unsaturated bonds than the more
saturated paraffin oil. This makes mineral oil more
unstable and prone to attack by free oxygen
radicals and photo-oxidation than stable paraffin
oil.
In a study by Tae et al. published in 2006, bovine
embryo development was studied in cultures
overlaid with either washed mineral or sterile
paraffin oil, Fig 1.
Percent
Most clinics today use oil to cover embryo culture
dishes in order to maintain stable temperature,
osmolality and pH. The quality of such oil plays a
very important role for IVF success. The oil types
commonly used are mineral oil, paraffin oil or a
mixture of both these oil types.
Embryo development
under different oil types
50
40
30
20
10
0
Morula
Blastocyst
Washed mineral oil
Sterile paraffin oil
Comparison Mineral oil and paraffin oil
Sterile filtered paraffin oil overlay resulted in
significantly higher (p < 0.05) development rate
to morula (44.8% versus 30.6%) and blastocyst
(32.8% versus 21.7%) than washed mineral oil.
REF: Tae, J.C. et al. J. Assist Reprod & Gen. 2006
Fig 1. Embryo development
under different oil types
Percent
The importance of oil
for IVF outcome
50
Washed mineral oil
Sterile paraffin oil
40
30
20
10
0
Morula
Washed mineral oil
Sterile paraffin oil
Blastocyst
Embryo development, per
60
ife
40
Superior embryo viability
with OVOIL™
40
20
20
0
Cook Medicult Sage
0
Cook Medicult Sage
Day 3
Day 4
Sigma
Vitrolife
Day 5
Day 5 hatching
Oil is not just oil
80
p<0.1
Fig 2. Embryo development
40 60
20 40
Sigma
Irvine Vitrolife
12.1
11.2
14.3
11.7
12.6
19.9
25.3
22.6
32.8
13.6
31.2
44.0
54.7
47.1
67.4
40.2
61.8
78.7
22.4
8.2
11.6
10.2
12.0
35.7
N=99 better
N=97 development
N=98
N=96(p<0.01)
N=97
N=97all
significantly
for
embryo viability parameters studied, number of
Day
Day 4
Dayand
5 in Day
5 hatching
cells
in3the trophectoderm
the inner
cell
mass, and total number of cells in the blastocysts.
Fig 3. Embryo viability
Cell number
60 80
Embryo development, percent
Cell number
Oils from 6 different manufacturers were tested
and embryo develop­ment and viability was
and Fig 2. OVOIL™ showed
Figevaluated.
3. EmbryoFig 1
viability
p<0.1
80
60
40
0 20
Cook Medicult Sage
Sigma
0 Trophectoderm
Total
Cook Medicult Sage Sigma
ife
Vitrolife
20
0
ICM
Irvine Vitrolife
Cook Medicult Sage
Sigma
Irvine Vitrolife
12.1
11.2
14.3
11.7
12.6
19.9
54
53
57
42
57
61
25.3
22.6
32.8
13.6
31.2
44.0
65
65
67
52
68
76
54.7
47.1
67.4
40.2
61.8
78.7
11
12
11
9
12
15
22.4
8.2
11.6
10.2
12.0
35.7
17%
18%
16%
18%
17%
20%
N=99
N=97
N=98
N=96
N=97
N=97
N=99
N=97
N=98
N=96
N=97
N=97
Day 3
Day 4
Day 5
Day 5 hatching
REF: D. Linck, SIRT, Australia. 2008.
%ICM
Trophectoderm
Total
ICM
REF: D. Linck, SIRT, Australia. 2008.
Fig 3. Embryo viability
Extensive quality testing
Cell number
1
60
80
p<0.1
OVOIL™ is an extensively quality tested product:
60
• Sperm
sensitivity testing
• 1-cell mouse embryo assays
40
• Sensitive CF1 mouse strain on raw material
• 1 cell, F1 hybrid mouse on finished product
• Mouse embryo assays with multiple
20
endpoints for grading development
• Blastocyst differentiation staining and cell
0counts
Cook Medicult Sage
%ICM
Sigma
P To learn more
about OVOIL™,
please check our
website at
www.vitrolife.com
Irvine Vitrolife
54
53
57
42
57
61
65
65
67
52
68
76
11
12
11
9
12
15
17%
18%
16%
18%
17%
20%
N=99
N=97
N=98
N=96
N=97
N=97
Vitrolife . Box 9080 . SE-400 92 Göteborg . Sweden . Tel +46 31 721 80 00 . Fax +46 31 721 80 90 . [email protected] . www.vitrolife.com
.
.
.
.
.
3601
South Inca Street . Englewood
Trophectoderm
Total Colorado
ICM 80110 USA Tel +1-303-762-1933 Fax +1-303-781-5615 [email protected]
© Vitrolife 1.130507.1
Embryo development, percent
80