University of Groningen
Crystal structure of Agaricus bisporus tyrosinase
Ismaya, Wangsa Tirta
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2011
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Ismaya, W. T. (2011). Crystal structure of Agaricus bisporus tyrosinase Groningen: s.n.
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Download date: 18-06-2017
Crystal Structure of Agaricus bisporus
Tyrosinase
Cover: wall on the grass
By: Wangsa Tirta Ismaya (2009)
The research was carried out at the Protein X-ray crystallography group, Laboratory of
Biophysical Chemistry, Groningen Biomolecular Science and Biotechnology Institute,
Faculty of Mathematics and Natural Sciences, University of Groningen, The Netherlands
The research project was in collaboration with Agriculture and Food Innovation BV,
Wageningen University and Research Center, and funded by Senter IOP (Industrial
Onderzoek Project, IEE0002).
RIJKSUNIVERSITEIT GRONINGEN
Crystal structure of
Agaricus bisporus tyrosinase
Proefschrift
ter verkrijging van het doctoraat in de
Wiskunde en Natuurwetenschappen
aan de Rijksuniversiteit Groningen
op gezag van de
Rector Magnificus, dr. E. Sterken,
in het openbaar te verdedigen op
vrijdag 24 juni 2011
om 14.45 uur
door
Wangsa Tirta Ismaya
geboren op 12 januari 1975
te Jakarta, Indonesië
Promotores
: Prof. dr. B. W. Dijkstra
Prof. dr. H. J. Wichers
Beoordelingcommissie : Prof. dr. ir. M. W. Fraaije
Prof. dr. E. Boekema
Prof. dr. L. Dijkhuizen
ISBN : 978-90-367-4904-6 (electronic version)
ISBN : 978-90-367-4905-3 (printed version)
For papi, mami, Lia, and Hery
and all my teachers
and all my friends
.
.
.
.
Contents
Chapter 1
An introduction to mushroom Agaricus bisporus tyrosinase
Page 1 – 23
Chapter 2
Overproduction, purification, and
mushroom tyrosinase
Page 25 – 40
refolding
of
recombinant
Chapter 3
Crystallization and preliminary X-ray crystallographic analysis of
mushroom Agaricus bisporus tyrosinase
Page 41 – 52
Chapter 4
Crystal structure of mushroom Agaricus bisporus tyrosinase –
identity of the tetramer and interaction with tropolone
Page 53 – 74
References
Page 75 – 81
Summary (English – Dutch – Indonesian)
Page 83 – 99
Acknowledgement
Page 101 – 102
.
.
.
.