Centrifuge
Operator’s Manual
Part of Thermo Fisher Scientific
582 Monroe Road, Suite 1424 Sanford, FL 32771
800.777.6668 ∙ 407.788.8791
www.separationtechnology.com
TABLE OF CONTENTS
SECTION 1
INTRODUCTION
1.1
Description and Intended Use
1.2
Testing Applications
1.3
Safety
SECTION 2
INSTALLATION
2.1
Unpacking
2.2
Power Requirements and Power Cord Installation
2.3
Rotor Installation and Removal
SECTION 3
OPERATING INSTRUCTIONS
3.1
Rotor Loading and Balancing
3.2
Use of Disposable Tube Holders
3.3
Start-Up
3.4
Stopping
3.5
Automatic Lid Lock - Manual Override
SECTION 4
MAINTENANCE
4.1
General Cleaning and Disinfecting
4.2
Rotor Cleaning and Disinfecting
4.3
Inspections
4.4
Speed Check
4.5
Timer Check
4.6
Service
SECTION 5
TROUBLESHOOTING
SECTION 6
ACCESSORIES
SECTION 7
WARRANTY
SECTION 8
SPECIFICATIONS
SECTION 9
GLOSSARY
1
SECTION 1
INTRODUCTION
1.1 DESCRIPTION AND INTENDED USE
The PlasmaPrep™ Centrifuge is a compact, sturdy device designed for the
rapid separation of blood cells from serum or plasma for a variety of laboratory
applications. The fixed-angle rotor will accommodate up to six specimen tubes
ranging in size from 2 to 10mL. With a speed of 7200±350 rpm (4227 RCF), it
has been designed with four precisely timed spin cycles:
• The PRP button provides platelet rich plasma in 30 seconds.
• The 2 MIN button provides a 2-minute intermediate spin.
• The 3 MIN button provides serum or plasma separation (Platelet Poor
Plasma) in 3 minutes.
• Pressing the 2 MIN and 3 MIN buttons simultaneously until the beep is
heard provides a 5-minute spin.
The centrifuge is not intended for use with materials other than blood.
To help ensure safety, the centrifuge is equipped with an automatic lid lock, which
prevents it from being opened while the rotor is in motion. A gasket seal is
mounted in the lid to minimize possible aerosol exposure. Disposable tube holders
provide another safety enhancement. In the event of tube breakage during
centrifugation, the operator can discard both the disposable tube holder and the
tube safely without making contact with the blood specimen and without cleaning
the centrifuge bowl or rotor.
To monitor proper operation, the centrifuge has LED indicator lights and an audible
alarm to signal both normal and abnormal operating conditions.
1.2 TESTING APPLICATIONS
A.
Platelet Poor Plasma (PPP)
When the 3 MIN button is pressed, the centrifuge will separate the red and
white cells and platelets from the plasma or serum.
• Coagulation
When an anticoagulated specimen is separated in the PPP mode, the
resulting high quality platelet poor plasma may be used for coagulation
studies such as prothrombin time activated partial prothrombin time,
thrombin and fibrinogen.
• Chemistry
Plasma or serum separated by the centrifuge may also be used for
chemistry analyte testing. The use of plasma or serum will depend upon
the chemistry procedure employed.
2
A 2-minute spin cycle is available for these or other applications. To
initiate, press the 2 MIN button.
A 5-minute spin cycle is available for these or other applications. To
initiate, press the 2 MIN and 3 MIN buttons simultaneously until the beep is
heard.
B.
Platelet Rich Plasma (PRP)
When the PRP button is pressed, the centrifuge is designed to remove the
red and white blood cells from the plasma leaving the platelets suspended
in the plasma. The resulting platelet rich plasma may be used for platelet
aggregation studies.
1.3 SAFETY
STI products are designed for convenient and reliable operation and to accepted
standards of safety. The use of the centrifuge does not entail any hazard if
operated in accordance with the instructions given in the manual. However,
incorrect actions by a user may damage the equipment or cause a hazard to
health. It is important to comply with the following safety precautions.
A. Electrical Safety
• Operate the centrifuge only at the line voltage and frequency specified
for your area and matched by the voltage selector switch on the rear of
the centrifuge.
• Always use a grounded electrical outlet.
• Unplug the power cord before attempting to clean the centrifuge.
B. Operational Safety
• All users must read and understand the Operator’s Manual and only
operate the instrument in accordance with the instructions. If the
instructions are not followed, then the protection provided by the
instrument may be impaired.
• For smooth operation and long centrifuge life, always balance the rotor
before starting a spin cycle.
• Use the proper disposable tube holders.
• Replace balance tubes after 10 spin cycles to avoid the possibility of tube
breakage due to repeated centrifugation stress.
• Never attempt to override the automatic lid lock mechanism during
operation.
• Use only the recommended cleaning procedure.
• Do not lean on centrifuge.
• Do not use with materials other than blood, including but not limited to,
flammable or explosive materials, materials that are known to be toxic,
radioactive, or contaminated with pathogenic micro-organisms.
3
C. Infectious Disease Protection
• Observe approved laboratory procedures when handling blood. It is
important that normal standards of safety and good laboratory practices
are employed.
• Always use protective gloves and face protection approved for laboratory
use when working with body fluids.
• Inspect specimen tubes before centrifugation. To avoid tube breakage,
blood collection tubes should be centrifuged as few times as possible.
Cracked, chipped or scratched tubes should not be used.
• If a tube breaks in the centrifuge, carefully remove the disposable tube
holder including broken tube with a hemostat or other appropriate device.
• If hazardous material is spilled on or inside the PlasmaPrep, the user
should carry out the appropriate decontamination.
SECTION 2
INSTALLATION
2.1 UNPACKING
The shipping box contains:
• Centrifuge
• Rotor
• Power cord
• Four disposable tube holders (two for use with 13 x 75mm rubber stopper
tubes and two for use with 13 x 75mm plastic top tubes)
• Operator’s manual
• Latch tool
• Helpful hints and tube holder guide
Place the centrifuge on a level work surface along with the other items contained in
the box.
2.2 POWER REQUIREMENTS AND POWER CORD INSTALLATION
The centrifuge has a built-in voltage selector switch which can be set for use on
either 110V, 60Hz or 220V, 50Hz current. Locate this switch on the back of the
centrifuge next to the power cord connector plug and check to see that it is set to
the proper voltage for your electrical outlet. In the United States, the proper setting
is 110V.
TO AVOID DAMAGE TO THE SELECTOR SWITCH, DO NOT
ATTEMPT TO CHANGE THE VOLTAGE WHILE THE POWER
CORD IS PLUGGED INTO AN ELECTRICAL OUTLET.
4
If the voltage selector switch is at the improper setting, unplug the unit, insert a flat
head screwdriver into the slot of the switch and turn it to the proper setting.
Plug the centrifuge power cord into the power connector at the rear of the device,
then into a grounded power outlet rated at the appropriate voltage.
To avoid equipment damage and/or electrical shock hazards, connect power cord
only to a three-wire grounded receptacle. When only a two-wire receptacle is
available, have it replaced with a properly grounded three-wire receptacle by a
qualified service person in accordance with your local electrical code. Do not
remove grounding prong from power cord. If an extension cord is required, use
only a three-wire grounded cord having proper voltage and current rating.
2.3 ROTOR INSTALLATION AND REMOVAL
The centrifuge is shipped without the rotor installed.
To install the rotor:
A. Unscrew the rotor knob from the spindle.
B. Align the 2 mounting pegs on the spindle with 2 of the small mounting holes
on the underside of the rotor as shown below.
C. Ensure that the rotor "seats" properly with the pegs fitted into the holes. When
properly seated, there will be approximately 3/8" of the threaded spindle
visible above the top of the rotor.
5
D. Remove the black rotor tool located in the compartment on the underside of
the centrifuge. Secure the rotor by positioning the tool in the holes on the top
of the rotor knob and turning clockwise to tighten.
FAILURE TO PROPERLY INSTALL THE ROTOR WILL RESULT
IN DAMAGE TO THE DEVICE AND VOID THE WARRANTY.
To prevent possible damage, it is important to remove the rotor whenever the
centrifuge is to be moved to another location. To remove the rotor:
A. Remove the black rotor tool located in the compartment on the underside of
the centrifuge.
B. Position the tool in the holes on the top of the rotor knob and turn
counterclockwise to loosen knob.
C. Continue to unscrew the knob by hand until it can be removed.
D. Lift the rotor vertically from the spindle.
SECTION 3
OPERATING INSTRUCTIONS
3.1 ROTOR LOADING AND BALANCING
THE ROTOR MUST BE BALANCED BEFORE THE SPIN CYCLE IS INITIATED.
To balance the rotor, tubes of equal weight with their respective disposable tube
holders must be placed opposite each other in the numbered rotor positions.
When centrifuging an odd number of tubes, place a balance tube of equal weight
with respective disposable tube holder opposite the odd tube. The balance tube
may be filled with water. Replace the balance tube after 10 spin cycles to avoid the
possibility of tube breakage due to repeated centrifugation stress.
If the centrifuge is being run severely out of balance, the spin cycle will be aborted,
the PRP and 2 MIN mode LEDs will flash simultaneously and an alarm will beep
until the on/off switch is cycled.
3.2 USE OF DISPOSABLE TUBE HOLDERS
The rotor will accommodate a variety of tube sizes routinely used for drawing 2
through 10mL specimens. Disposable tube holders provide support for the tubes to
help prevent breakage and contain specimen and tube fragments in the event of a
tube failure.
6
DISPOSABLE TUBE HOLDERS ARE REQUIRED FOR ALL
TUBES. THE SIZE AND TYPE OF THE TUBES USED MUST
MATCH THE DISPOSABLE TUBE HOLDER SIZE AND TYPE.
Additional disposable tube holders are available. As draw volume may vary,
refer to the physical tube size and closure type to select the proper tube holder.
Use recommended disposable tube holders only. Tube cushions and/or other
manufacturer’s tube adapters should not be used.
Disposable tube holders for the rotor:
Tube Size & Type
Tube Holder Required
10.25 x 47 mm
Rubber Stopper
Yellow -
Catalog #230-110
10.25 x 64 mm
Rubber Stopper
Red -
Catalog #230-111
13 x 75 mm
Rubber Stopper
Blue -
Catalog #230-112
13 x 75 mm
Plastic Top
Silver -
Catalog #230-122
13 x 100 mm
Rubber Stopper
Pink -
Catalog #230-114
13 x 100 mm
Plastic Top
Gold -
Catalog #230-115
16 x 100 mm
Rubber Stopper
Green -
Catalog #230-116
The rotor must be empty (no cushions, etc.) before inserting the disposable tube
holders. This can be verified by tapping the bottom of each rotor well with a pencil.
When the lid is closed verify that the tops of the tubes are not touching the lid.
Replace disposable tube holders monthly or more frequently as needed. Cleaning
the disposable tube holders is not recommended. Should a tube break during
centrifugation, simply remove the entire disposable tube holder and tube using a
hemostat or other appropriate device. Discard the affected tube and disposable
tube holder in accordance with proper laboratory procedures and replace with a
new disposable tube holder.
The centrifuge will not accommodate tubes with dimensions larger than 16 x
100mm. The centrifuge does not accept gel separator tubes.
ALWAYS INSERT THE PROPER DISPOSABLE TUBE
HOLDERS BEFORE LOADING THE SPECIMEN TUBES
7
ALWAYS INVERT ANTICOAGULATED TUBES TWO OR
THREE TIMES BEFORE INSERTING THEM INTO THE ROTOR
TO FACILITATE ACHIEVING LOW RESIDUAL PLATELET
COUNTS.
THE ROTOR MUST BE PROPERLY BALANCED BEFORE THE
SPIN CYCLE IS INITIATED. IMPROPER BALANCING MAY
CAUSE DAMAGE TO THE CENTRIFUGE.
3.3 START-UP
Make sure the centrifuge power switch located at the rear of the device is in the
ON position. There is a light (LED) located on the top right side of the centrifuge
and it will be GREEN. This light is called the status LED.
Before the spin cycle is engaged, lock the lid by pressing down firmly on the lid
tab. The PRP, 2 MIN and 3 MIN buttons are used to start the spin cycle. There
is a GREEN light (LED) located above each button. These three lights are
called mode LEDs.
Press the PRP, 2 MIN or 3 MIN button to begin desired spin cycle:
• PRP provides platelet rich plasma in 30 seconds. The LED over the
PRP button will illuminate when the button is pressed.
• 2 MIN provides a 2 minute intermediate spin. The LED over the 2 MIN
button will illuminate when the button is pressed.
• 3 MIN provides serum or plasma separation (Platelet Poor Plasma) in
3 minutes. The LED over the 3 MIN button will illuminate when the
button is pressed.
• An additional spin cycle of 5 minutes can be accessed by pressing the
2 MIN and 3 MIN buttons simultaneously until the beep is heard. The
LEDs over the 2 MIN and 3 MIN will illuminate when both buttons are
pressed simultaneously.
The GREEN status LED will turn RED and the motor will start.
3.4 STOPPING
A.
Normal Operation
When the spin cycle is completed, the rotor will decelerate, the GREEN mode
LED will turn OFF and the rotor will stop within 60 seconds. After the rotor
has come to a complete stop, a series of three BEEPS will be heard, and the
status LED will turn from RED to GREEN to indicate that the spin cycle is
complete and the automatic lid lock has disengaged to allow the lid to be
opened.
B.
Spin Cycle Abort
The centrifuge spin cycle can be aborted at any time during operation by
8
simply pressing either the PRP or the 2 MIN button. If the cycle is aborted,
the GREEN mode LED over the PRP and the 2 MIN button will flash. When
the rotor comes to a complete stop, the RED status LED will turn GREEN and
the lid lock will automatically release.
ALWAYS WAIT FOR THE ROTOR TO STOP SPINNING, THE
THREE BEEPS TO BE HEARD AND FOR THE STATUS LED
TO TURN GREEN BEFORE TRYING TO OPEN THE LID.
NEVER ATTEMPT TO OPEN THE LID DURING OPERATION
OR WHEN THE STATUS LED IS RED.
3.5 AUTOMATIC LID LOCK - MANUAL OVERRIDE
You may manually override the automatic lid lock by turning the power switch off,
waiting 5 seconds and turning it back on, or by inserting the enclosed L - shaped
latch tool into the small hole on the right side of the centrifuge, pushing gently until
the lid opens.
DO NOT ATTEMPT TO OPEN THE CENTRIFUGE UNTIL THE
ROTOR HAS COME TO A COMPLETE STOP AND THE STATUS
LED HAS TURNED GREEN.
SECTION 4
MAINTENANCE
4.1 GENERAL CLEANING AND DISINFECTING
As with all electrical devices, make sure the centrifuge is unplugged before
cleaning. Always wear protective clothing when using any cleaning materials.
NEVER USE BLEACH, ABRASIVES OR CORROSIVE
SOLVENTS TO CLEAN.
DO NOT SPRAY OR ALLOW ANY LIQUID TO GET INSIDE
THE CENTRIFUGE. LIQUID WILL HARM THE ELECTRONICS.
SUBSEQUENT PROBLEMS WILL NOT BE COVERED UNDER
WARRANTY.
Use a cloth slightly dampened with a non-corrosive disinfectant solution to clean
9
the lid and other parts of the centrifuge housing. Dry all surfaces with a soft tissue
or cloth after cleaning.
4.2 ROTOR CLEANING AND DISINFECTING
Clean the rotor by immersing it in a non-corrosive disinfectant. To disinfect, soak
for 20 minutes. Rinse and dry the rotor thoroughly before re-installing it.
4.3 INSPECTIONS
Periodically inspect the rotor, lid and the lid gasket to ensure there are no cracks or
damage.
4.4 SPEED CHECK
The centrifuge utilizes microprocessor technology, which internally monitors the rpm
at 7200 ± 350. The centrifuge should reach its operating rpm in approximately 25
seconds. If the speed should fall outside of the specified range during normal
operation, the rotor will decelerate and stop, and the GREEN Mode LEDs over the
PRP, 2 MIN and 3 MIN buttons will flash alternately. The audible alarm will also
BEEP continuously for about 4 seconds. Cycle the power by turning the centrifuge to
the OFF and then ON position. If the spin cycle is engaged again and the rpm is still
out of range, the visual and audible alarms will be repeated. The centrifuge should
be turned OFF and removed from service.
4.5 TIMER CHECK
The spin time is acceptable if it is within ± 3 seconds of factory set spin time for
the mode.
4.6 SERVICE
For service, please contact Separation Technology Customer 1.800.777.6668.
All instruments or accessories must be cleaned and decontaminated prior to
shipment to the manufacturer for service. This decontamination is required by
Federal law and EPA regulations. Employees of Separation Technology cannot
perform this decontamination.
10
Symptom
Mode LED flashes when
MODE button is pressed and
the device will not spin.
Lid will not open.
PRP and 2 MIN LEDs flashing
simultaneously and alarm
BEEPS until unit is turned off.
PRP, 2 MIN and 3 MIN LEDs
flashing alternately and
alarm BEEPS for 4 seconds.
PRP and 2 MIN Mode LEDs
flashing simultaneously.
Tubes will not seat properly in
rotor tube well or disposable
tube holder.
Tubes breaking during
centrifugation.
Centrifuge vibrating
abnormally during spin cycle.
Centrifuge will not operate.
SECTION 5
TROUBLESHOOTING
Problem
Solution
Lid not locked.
Press down firmly on lid tab.
Lid lock is engaged.
Disengage lid lock.
(See Section 3.5)
Device is in the spin cycle.
Allow spin cycle to end.
(See Section 3.4)
Rotor out of balance
Balance rotor.
(See Section 3.1)
Loose rotor knob.
Tighten rotor knob.
Rpm’s are out of specified range.
Check rotor speed.
(See Section 4.4)
Spin cycle has been aborted and
lid lock is still engaged.
Lid was opened during the spin.
Disengage lid lock and resume
normal operation.
(See Section 3.5)
Improper disposable tube holder
used.
Use proper disposable tube
holder. (See Section 3.2)
Foreign objects in the rotor well
or tube holder
Remove foreign objects in the
rotor well or tube holder.
(See Section 3.2)
Improper disposable tube holder
used.
Use proper disposable tube
holder. (See Section 3.2)
Poor quality or overstressed
tubes.
Inspect tubes. (See Section
3.2)
Rotor out of balance.
Balance rotor. (See Section.
3.1)
Rotor not balanced.
Balance rotor. (See Section
3.1)
Centrifuge power switch is OFF.
Turn power ON.
Centrifuge not plugged into
electrical outlet.
Plug power cord into electrical
outlet.
Voltage selector switch not set
properly.
Select proper input voltage for
your area. (See Section 2.2)
Blown fuse.
Replace fuse with proper
replacement type. (See Sec. 8)
No power at outlet.
Call facility maintenance.
If the PlasmaPrep Centrifuge fails to operate correctly after following the above procedures, call Separation
Technology Customer Service at 800..777.6668.
11
SECTION 6
ACCESSORIES
Catalog No.
630-101
640-101
630-102
550-100
230-110
230-111
230-112
230-122
230-114
230-115
230-116
Description
Rotor
Rotor knob
Rotor tool
120V Power cord 3 prong NEMA 5-15P Plug
Yellow disposable tube holders, 25/pkg.
For use with 10.25 x 47mm rubber stopper tubes
Red disposable tube holders, 25/pkg.
For use with 10.25 x 64mm rubber stopper tubes
Blue disposable tube holders, 25/pkg.
For use with 13 x 75mm rubber stopper tubes
Silver disposable tube holders, 25/pkg.
For use with 13 x 75mm plastic top tubes
Pink disposable tube holders, 25/pkg.
For use with 13 x 100mm rubber stopper tubes
Gold disposable tube holders, 25 pkg.
For use with 13 x 100mm plastic top tubes
Green disposable tube holders, 25/pkg.
For use with 16 x 100mm rubber stopper tubes
12
SECTION 7
WARRANTY
SEPARATION TECHNOLOGY, INC. (STI) warrants each new PlasmaPrep™
Centrifuge (the Product) against defects in materials or workmanship for a period
of two years from the date of purchase and agrees to repair or replace any
defective Product without charge. This warranty does not cover damage resulting
from accident, misuse, lack of reasonable care, improper cleaning, improper
maintenance or improper packaging for return shipment to STI. This warranty
shall be void if the Product is repaired by anyone other than STI. This warranty
does not extend to anyone other than the original purchaser or to accessories
manufactured by other vendors.
Except as provided herein, STI makes no warranties of any kind, either express or
implied and specifically excluding any warranty of merchantability or warranty of
fitness for a particular purpose.
STI will not be liable for any special, consequential or incidental damages arising
out of the use or inability to use the Product. In no event shall STI's liability
hereunder exceed the purchase price of the Product. This warranty shall be void
and of no force and effect with respect to any Product which is damaged as a
result of a) neglect, alteration, electric current fluctuation or accident, b) improper
use, including failure to follow proper operation and maintenance, and to provide
proper environmental conditions prescribed in STI's Product operator’s manuals,
c) repair by other than STI or authorized service agents appointed by STI and
acting in accordance with STI's service announcements or d) use of supplies or
parts which do not meet STI specifications.
To obtain warranty service, call Separation Technology, Inc. at 800.777.6668.
13
SECTION 8
SPECIFICATIONS
Model no.:
Recommended use:
Rotor:
Maximum rotor capacity:
100-129
Centrifugation of blood
Six-place fixed
Six 10mL (16 x 100mm) specimen tubes filled
(including tube holders)
Tube capacity:
Six specimen tubes will accommodate
10.25 x 47mm through 16 x 100mm with proper
disposable tube holders
Motor:
Sealed, DC
Timer:
Platelet Rich Plasma (PRP) mode – 30 seconds
Intermediate Spin (2 MIN) – 2 minutes
Platelet Poor Plasma (3 MIN) – 3 minutes
Hard Spin – 5 minutes
ON/OFF switch:
Located at the rear of the device
Status LED:
GREEN light signals power ON condition and lid
can be opened. RED light signals run cycle in
progress and lid lock mechanism is engaged
Mode LEDs:
LED indicator for each operational mode (PRP, 2
MIN and 3 MIN); LEDs act in sequence as error
alerts as well
Audible alarm:
Alerts operator to end of run, run abort or low/high
rpm condition
Rpm and RCF:
7200 ± 350 rpm, 4227 RCF
Ramp-up time:
25 seconds
Electrical supply voltage: 120V 60Hz ± 10% 150W
120V Fuse, 1.25 amp (5 x 20mm) Type 218 Slo Blo
230V 50Hz ± 10% 150W
230V Fuse, .63 amp (5 x 20mm) Type 218 Slo Blo
Power cord:
Grounded cord with 3-prong NEMA 5-15p plug
Dimensions:
10"W x 15"L x 10"H
Bench area requirements: 15"W x 18"L x 20"H
Weight:
15.80 lb; shipping weight 22 lb
Temperature Range:
Operating 10°C to 40°C (50°F to 104°F)
Storage -10°C to 60°C (14°F to140°F)
Conditions:
For indoor use only.
Maximum relative humidity 95% non-condensing
Approvals:
ETL/CETL Listing for North America:
UL 61010-1
CAN/CSAC22.2#61010-1
IEC 61010-1
CENELEC EN 61010-1
IEC 61010-2-020
14
SECTION 9
GLOSSARY
SYMBOL
USED FOR
SYMBOL
USED FOR
There may be a
biohazard associated
with the instrument
Temperature
Limitation
Caution. Instructions
must be followed for
safe and correct
operation.
Serial Number
Consult Instructions
For Use
Catalog Number
Manufacturer
15
910-107G
03/2011
Manufactured by:
Separation Technology, Inc.
582 Monroe Road, Suite 1424
Sanford, FL 32771
www.separationtechnology.com
Made in USA