Cell Culture
LIVE
Volume 7
The Cell Culture Company
20 Years of Quality and Service
Leukemia Inhibitory Factor
Cell Counting
Cell Separation
Analysis of Protein Expression
by SILAC
Human Sera
P. 3
P. 4
P. 5
P. 6
P. 7
Cell Culture Live 7
20 Years of Quality and Service
Over the course of the last 20 years, PAA Laboratories has grown into
an international operation with manufacturing and sales on three continents. Our high quality cell culture products are manufactured in our
FDA registered and GMP certified production facilities, among the most
modern of its kind in the world. Worldwide presence provides PAA with
maximum flexibility to meet the requirements of our customers.
Our investment in the future
PAA’s cell culture media and buffers in powder form have been on the
market since 2009. We are not just content with having the most modern powder mill in operation; we are also prepared for future quality
standards by having implemented Process Analytical Technology (PAT).
Special product manufacturing and customised fillings are also part of
our service.
In 2008, we expanded our standard product range with disposables
such as plastic labware to give our customers the advantage of purchasing their laboratory supplies conveniently from one source.
With our new product line "ATMP-Ready", we are setting a new standard for raw materials in the manufacture of advanced therapy medical
products.
We are here for you
Customer orientation and reliability are not just phrases but good practice. Instead of anonymous call centres, our friendly customer services
team attends to you personally.
Our qualified technical service as well as your local sales representative
is always ready to assist you.
Enjoy your new Cell Culture Live.
Harry Brack
(Sales and Marketing Director)
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PAA – The Cell Culture Company | www.paa.com
Cell Culture Live 7
Leukemia Inhibitory Factor
Leukemia Inhibitory Factor (LIF) is a polyfunctional cytokine of the interleukin (IL)-6-family, which shows
pleiotropic activities in a variety of tissues and cell types. LIF is naturally produced by epithelial cells of the intestine, uterus, lung or kidney and has pro-and anti-inflammatory properties. Leukemia inhibitory factor binds to
the specific LIF receptor and forms a heterodimer with a specific subunit for gp130. By building a trimeric complex with it LIF activates a specific signaling pathway.
As a lymphoid growth factor LIF has an impact on the cell cycle, cell viability, proliferation and differentiation
of various cell types.
Convenient ready to use solution
Applications
PAA offers recombinant mouse LIF (mLIF) as a ready to use
solution in 100 µl and 1 ml pack sizes with highest
Murine cell research
Growth of different cell types
activity.
A 100 µl solution (equivalent to106 units) is sufficient to
supplement 1000 ml of the culture media. With a 1 ml
solution (equivalent to 107 units) 10 litres of culture media
can be supplemented.
Features
High purity (>95%)
Highest activity
Highest stability
Order Information
Very low endotoxin
Leukemia Inhibitory Factor (mLIF),
mouse, recombinant
Ready to use solution
F0001-008
100 µl
(106 units)
Source
Leukemia Inhibitory Factor (mLIF),
mouse, recombinant
Recombinant mouse LIF (mLIF) produced in E. coli is a
F001-008
1 ml
(107 units)
single, non-glycosylated, polypeptide chain containing
180 amino acids and has a molecular mass of 20 kDa.
After purification, the LIF is reconstituted in sterile PBS
containing 0.5 % BSA.
PAA – The Cell Culture Company | www.paa.com
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Cell Culture Live 7
Cell Counting
C-Chip – The New Way of Cell Counting!
Cell counting chambers are basic equipment for cell culture work in the laboratory. Traditional counting chambers
are made of glass, which require coverslips making sample
preparation a very time consuming process. The novel
C-Chip is made of robust plastic with two counting chambers fitted with a clear grid pattern for manual cell counting. It is easy to use and enables reproducible results
through precise volume control. Extensive purification
Features and Benefits
Easy to use
Precise and reproducible quantification
Clear grid pattern
No need for cover slips
No need for cleaning and re-use
Time and cost saving
steps are no longer necessary.
Applications
Cell Culture – counting and viability
Haematology – counting of erythrocytes and leukocytes
Microbiology – bacterial and fungal spore counting
IVF, IUS – sperm counting
Detection area
Sample injection
Order Information
C-Chip Neubauer Improved
PAA505050X
50 pieces
Brand-New:
C-Chip Malassez
Cat-No. PAA404040X
50 pieces
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PAA – The Cell Culture Company | www.paa.com
Cell Culture Live 7
Cell Separation
The isolation of cells or subcellular particles is the first step for investigations in diagnostics, cell-functionality
or gene expression. PAA offers two products for an optimal separation result.
LSM 1077 –
the well-proven classic
Blood Separation Tubes –
for precise and easy handling
LSM 1077 is a Ficoll based separation medium with a den-
With Blood Separation Tubes, separation of cells and cell
sity of 1.077 g/ml for the separation of blood cells.
organelles is significantly simplified. These special cen-
Optimal osmotic conditions and a physiological pH value
trifuge tubes contain a porous barrier, which separates the
ensure that the vitality and specific function of cells are not
sample material from the separation medium and prevents
affected. For precise and easy handling, we recommend
recontamination after centrifugation. The results are
the use of LSM 1077 in combination with Blood Sepa-
enriched cell fractions separated according to their densi-
ration Tubes.
ty which can be easily extracted for further experiments.
Features and Benefits
Features and Benefits
Optimal physiological parameters
Simplified filling
No diffusion through biological membranes
Precise and easy harvesting
High density with low viscosity
No recontamination with unwanted blood components
Sterile ready to use solution
Sterilised by gamma irradiation
Order Information
LSM 1077
Order Information
J11-004
100 ml
J15-004
500 ml
Blood Separation Tubes (50 ml) PAA535710
PAA535710X
25 pcs.
500 pcs.
Blood Separation Tube
before (left) and after centrifugation (right)
Blood Plasma Phase
Blood
Interphase (Lymphocytes)
Porous Barrier
LSM 1077
Porous Barrier
LSM Phase
Pellet (Erythrocytes)
PAA – The Cell Culture Company | www.paa.com
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Cell Culture Live 7
Analysis of Protein Expression by SILAC
SILAC Media
SILAC (Stable Isotope Labelling by Amino Acids in Cell
Culture) proved to be a very powerful mass spectrometry
technique for the investigation of protein-protein interactions for example. The method is based on the in vivo
Applications
Proteomics
Quantitative analysis of relative changes in
protein expression
incorporation of isotope-labelled amino acids into new syn-
Quantitative analysis of proteins for which
thesised proteins. It was developed to identify differences in
no antibodies are available
protein abundance between two (or more) samples. Two
populations of cells are cultivated either with amino acids
consisting of natural "light" isotopes or those containing
Protein expression of normal cells compared
to abnormal cells
corresponding "heavy" ones. Proteins from both cell pop-
Identification and quantification of a high number
ulations can then be combined and analysed by mass
of proteins in a single experiment
spectrometry.
PAA offers two cell culture media, SILAC DMEM and SILAC
Analysis of signal pathways
RPMI 1640, which have been developed for this particular
application. They contain neither L-Lysine nor L-Arginine.
The labelled amino acids must be added before use.
Features and Benefits
100% label incorporation into proteins of living cells
Dialysed FBS
For non-radioactive as well as for radioactive incorporation
studies, dialysed FBS is particularly suitable as a growth
No intra-experimental variability caused by different
sample preparations
Media deficient in both L-Lysine and L-Arginine
supplement. This serum is dialysed with a 10 kDa cut-off
against 0,15 M salt solution to reach a significant reduction of amino acids.
Order Information
SILAC DMEM, without L-Glutamine
E15-086 500 ml
SILAC RPMI 1640, without L-Glutamine E15-087 500 ml
Dialysed FBS (EU approved)
A11-107 100 ml
A15-107 500 ml
Dialysed FBS (USA origin)
A11-207 100 ml
A15-207 500 ml
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PAA – The Cell Culture Company | www.paa.com
Cell Culture Live 7
Human Sera
PAA offers a wide range of blood products used in
research and diagnostics. Our raw materials come exclu-
Your benefits at a glance
Autologous system: surface molecules and expression
sively from accredited blood centres to guarantee the
patterns are not falsified by growth factors foreign to
highest safety and quality possible. All blood donations are
the species
tested for possible infection with HIV1 and 2, hepatitis B
and C and syphilis in accordance with current regulations.
More extensive analyses are made in our in-house Quality
Control procedures.
Various applications: sensitive human cell lines but
also primary cells such as lymphocytes or macrophages benefit from the use of human serum
Maximum safety: human serum is only obtained from
PAA is able to offer the best product for your individual
applications because of different treatments such as defibrination, dialysis, delipidation and charcoal stripping.
accredited blood centres and strictly tested
Off The Clot serum has run through the natural
coagulation process and contains no fibrinogen
Type AB serum does not contain A or B antibodies
and is therefore suitable for sensitive test systems
Order Information
We offer sterile products especially for use in cell culture
characterised by the words "Cell Culture Grade". Our customers from the diagnostic industry may choose from the
Human Serum, Off the Clot,
C11-020
100 ml
Cell Culture Grade
C15-020
500 ml
Human Serum, Off the Clot,
C11-021
100 ml
Type AB, Cell Culture Grade
C15-021
500 ml
group of "Diagnostic Grade” products. For more information please go to:
www.paa.com/products/humanbloodproducts.html
Optimal culture conditions
Human Serum, Converted,
C11-001
100 ml
Cell Culture Grade
C15-001
500 ml
Human Serum, Converted,
C11-002
100 ml
Type AB, Cell Culture Grade
C15-002
500 ml
Human serum in cell culture medium provides human cell
lines with an environment more closely matching their
physiological conditions than media containing animal
sera. By using human serum instead of fetal bovine serum
(FBS), human cells are naturally stimulated. The use of FBS
can affect the expression patterns of surface molecules.
Human Serum Plus, Off the Clot,
C11-011
100 ml
Type AB, Cell Culture Grade
C15-011
500 ml
Complex inter- and intracellular processes may also vary
depending on the use of autologous serum or FBS because
Note: PAA’s Human Sera should only be used for in vitro diagnostic and research
purposes and are not intended for therapeutic use.
of other growth factors. In particular, primary cells such as
lymphocytes are preferentially cultured in human serum to
eliminate potential modifications of the cells.
PAA – The Cell Culture Company | www.paa.com
7
Austria
Germany / The Netherlands
United Kingdom
France
PAA Laboratories GmbH
PAA-Strasse 1
4061 Pasching
PAA Laboratories GmbH
Unterm Bornrain 2
D-35091 Cölbe
PAA Laboratories Ltd
Termare Close
Houndstone Business Park
Yeovil, Somerset BA22 8YG
PAA Laboratories
67 rue Aristide Briand
78130 Les Mureaux
Tel.: (+43) 7229 64865
Fax: (+43) 7229 64866
[email protected]
Tel: (+49) 6421-17 53 90
Fax: (+49) 6421-17 53 918
[email protected]
Tel: (+44) 1935 411 418
Fax: (+44) 1935 411 480
[email protected]
Tel: (+33) 1 300 415 03
Fax: (+33) 1 300 415 06
[email protected]
www.paa.com