AnnaKarnkowska,PhD
EDUCATION
2006-2011
PhDinMolecularEvolutionandTaxonomy
FacultyofBiology,UniversityofWarsaw,Poland
Thesis:Phylogenyandtaxonomyofautotrophiceuglenids(Euglenea)basedon
molecularandmorphologicaldata
Supervisor:Prof.BożenaZakryś
2004-2006
MSc(Hons)inBiologyofthecellandorganism
FacultyofBiology,UniversityofWarsaw,Poland
2001-2004
BSc(Hons)inBiologyofthecellandorganism
FacultyofBiology,UniversityofWarsaw,Poland
PROFESSIONALEXPERIENCE
AssistantProfessor
DepartmentofMolecularPhylogenetics&Evolution,UniversityofWarsaw,
Poland
2016
Postdoctoralresearchfellow
BotanyDepartment,UniversityofBritishColumbia,Vancouver,Canada
Supervisor:Prof.PatrickKeeling
2011-present
2013–2015
2014
Postdoctoralresearchfellow
ParasitologyDepartment,CharlesUniversityinPrague,CzechRepublic
Supervisor:Drhab.VladimirHampl
Visitingresearcher
DepartmentofCellBiology,UniversityofAlberta,Canada(6months)
Supervisor:Prof.JoelB.Dacks
2014
Visitingresearcher
Dept.ofBiochemistry&MolecularBiology,DalhousieUniversity,Canada(2
weeks)
Supervisor:Prof.AndrewJ.Roger
2009
Visitingresearcher
Ehrenbergcollection,MuseumfürNaturkunde,Germany(2weeks)
Visitingresearcher
Dept.ofPlantBiology,MichiganStateUniversity,USA(2months)
Supervisor:Prof.RichardE.Triemer
Studentvolunteer
LaboratoryofBioinformaticsandProteinEngineering,InternationalInstituteof
MolecularandCellBiology,Poland(3months)
Supervisor:Prof.JanuszM.Bujnicki
2006
2004
AWARDSANDFELLOWSHIPS
2016
UniversityofWarsawRectorTeamAwardforResearchAchievement
2016
2015
2015
PostdoctoralResearchFellowshipofCenterforMicrobialDiversityandEvolution
(CMDE)
PhylogeneticallyinformedcurationofEukaryotic18SrDNA(EukRef)workshop
fellowship(Vancouver,Canada).
Holz-ConnerTravelAwardfromISOPforISOPConference(Sevilla,Spain)
2015
FEMSYoungScientistsMeetingGrantforEuropeanPhycologicalCongress
(London,UnitedKingdom)
EukaryoticCellJournalOutstandingYoungInvestigatorAwardattheEMBO
ComparativeGenomicsofEukaryoticMicroorganismsconference(SanFeliude
Guixols,Spain)
2013
2013-2015
PostdoctoralResearchFellowshipfundedbyEuropeanSocialFundandthestate
budgetoftheCzechRepublic
2013-2014
YoungScientistAwardoftheFoundationforPolishScience
2012-2013
AwardforthebestyoungPhDsoftheUniversityofWarsaw
2012-2013
PolishPrimeMinisterAwardforthedoctoralthesis
2012
Holz-ConnerTravelAwardfromISOPforProtist2012Conference(Oslo,Norway)
2011
UniversityofWarsawRectorAwardforthedoctoralthesis
2011
FoundationforPolishScienceTravelAwardforVIEuropeanCongressof
Protistology(Berlin,Germany)
2009
UniversityofWarsawRectorAwardforResearchAchievement
2009
TravelAwardfrom9thInternationalPhycologicalCongressorganizers(Tokyo,
Japan)
PhDscholarshipfundedbyFacultyofBiology,UniversityofWarsaw
2006-2010
RESEARCHGRANTS
ASAPRINCIPALINVESTIGATOR
2017-2020
PlastidevolutionandfunctionsofcolourlessalgaewithintheEuglenophytesand
Dictyochophyceae,PolishNationalScienceCentergrant
2012-2013
Occurrenceanddistributionofconventionalandnon-conventionalintronsintubB
geneofheterotrophiceuglenids,UniversityofWarsawintramuralgrant
Taxonomyofgreeneuglenids-insightintotheEhrenbergCollection,UEgrantin
Synthesysprogram,MuseumfürNaturkunde,Berlin
OriginofthechloroplastofgreeneuglenidsbasedonthephylogenyofpsbOgene,
UniversityofWarsawintramuralgrant
EvolutionofgapCgeneinEuglenida,UniversityofWarsawintramuralgrant
2009
2009-2010
2008-2009
2007-2008
Analysisofvalueofcox1sequenceintaxonomyandphylogenyofeuglenids,
UniversityofWarsawintramuralgrant
ASATEAMMEMBER
2013-2015
GenomesequencingofoxymonadandTrimastix.PI:DrVladimirHampl,Charles
UniversityinPrague,CzechRepublic
2011-2014
Distributionofconventionalandnon-conventionalintronsinnucleargenesof
photosyntheticeuglenids,PolishNationalScienceCentergrant.
PI:DrRafałMilanowski,UniversityofWarsaw,Poland
2011-2014
2011-2013
2007-2010
Molecularidentificationofgreeneuglenidsandtaxonomicverificationofcryptic
species,MinistryofScienceandHigherEducationgrant.PI:Prof.BożenaZakryś,
UniversityofWarsaw,Poland
ThepotentialandrealizedtoxicityofcyanobacteriainthewatersoftheGreat
MazurianLakesonthebasisofmolecularmarkersanalysis,concentrationsof
toxinsandenvironmentalanalysis,PolishMinistryofScienceandHigher
Educationgrant.
PI:DrIwonaJasser,UniversityofWarsaw,Poland
Phylogenyandtaxonomyofeuglenids(Euglenaceae)basedonmolecularand
morphologicaldata,MinistryofScienceandHigherEducationgrant.
PI:Prof.BożenaZakryś,UniversityofWarsaw,Poland
LISTOFPUBLICATIONS
1. NovakL,ZubáčováZ,KarnkowskaA,KoliskoM,HroudováM,StairsCW,SimpsonAGB,Keeling
PJ,RogerAJ,ČepičkaI,HamplV(2016)Argininedeiminasepathwayenzymes:evolutionary
historyinmetamonadsandothereukaryotes.BMCEvolBiol16:197(IF:3,407;MNiSW:30)
2. KarnkowskaA,HamplV(2016)Thecuriouscaseofvanishingmitochondria.MicrobialCell
3(10):361-364.
3. Łukomska-KowalczykM*,KarnkowskaA*,KrupskaM,MilanowskiR,ZakryśB(2016)DNA
BarcodingInAutotrophicEuglenids:EvaluationofCOIand18SrDNA.JPhycol52:951-60.
*bothauthorscontributedequally
4. KarnkowskaA.,VacekV.,ZubacovaZ.,TreitliSC.,PetrzelkovaR.,EmeL.,NovakL.,ZarskyV.,
BarlowLD.,HermanEK.,SoukalP.,HroudovaM.,DolezalP.,StairsCW.,RogerAJ.,EliasM,
DacksJB.,VlcekC.andHamplV.(2016)AEukaryotewithoutaMitochondrialOrganelle.
CurrBiol26,1274–1284.
Dispatch:BurkiF(2016)MitochondrialEvolution:Going,Going,Gone.CurrBiol10,
R410-R412.
5. MilanowskiR,GumińskaN,KarnkowskaA,IshikawaT,ZakryśB(2016)Intermediateintrons
innucleargenesofeuglenids-aretheyadistincttype?BMCEvolBiol16:49.
6. Łukomska-KowalczykM,KarnkowskaA,MilanowskiR,ŁachŁ,ZakryśB(2015).Delimiting
speciesinthePhacuslongicaudacomplex(Euglenida)throughmorphologicaland
molecularanalyses.JPhycol51:1147-57.
7. KarnkowskaA,BennettMS,WatzaD,KimJI,ZakryśB,Triemer,RE.(2015)Phylogenetic
relationshipsandmorphologicalcharacterevolutionofphotosyntheticeuglenids
(Excavata)inferredfromtaxon-richanalysesoffivegenes.JEukMicrobiol62:362-73.
8. BukowskaA,BielczyńskaA,KarnkowskaA,ChróstRJ,JasserI(2014)Calibrationofthe
analysesoftaxonomiccompositionofpotentiallytoxiccyanobacteriainfreshwaterlakes
9.
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11.
12.
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16.
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20.
usingamolecularapproachbasedonDGGEandclassicalmicroscopicobservations.Aquatic
Biosystems10:2.
MilanowskiR,KarnkowskaA,IshikawaT,ZakryśB(2014)Distributionofconventionaland
non-conventionalintronsintubAandtubBgenesofeuglenids.MolBiolEvol31:584-93.
ZakrysB,Karnkowska-IshikawaA,Łukomska-KowalczykM,MilanowskiR(2013)New
photosyntheticeuglenoidisolatedinPoland:Euglenariaclepsydroidessp.nova(Euglenea).
EurJPhycol48:260-7.
JasserI,Karnkowska-IshikawaA,ChróstRJ(2013)Doacid-tolerantpicocyanobacteriaexist?
AstudyoftwostrainsisolatedfromhumiclakesinPoland.Hydrobiologia707:209-18.
Karnkowska-IshikawaA,MilanowskiR,TriemerRE,ZakryśB.(2013)Arediscriptionof
morphologicallysimilarspeciesfromthegenusEuglena:E.laciniata,E.sanguinea.E.
sociabilisandE.splendens.JPhycol49:616-26.
Karnkowska-IshikawaA,MilanowskiR,TriemerRE&ZakryśB(2012)Taxonomicrevisionsof
morphologicallysimilarspeciesfromtwogenera:Euglena(E.granulataandE.velata)and
Euglenaria(Eu.anabaena,Eu.caudata,Eu.clavata).JPhycol48:729-39.
Karnkowska-IshikawaA,MilanowskiR,ZakryśB(2011)ThespeciesEuglenadeses
(Euglenaceae)revisited:newmorphologicalandmoleculardata.JPhycol47:653-61.
JasserI,KrólickaA,Karnkowska-IshikawaA(2011)Thegeneticdiversityandphylogenyof
picocyanobacteria:anewcladefromMazurianlakesreflectingearlyontogenicstagesof
glaciallakes.FEMSMicrobiologyEcology:75(1):89-98.
LintonEW,Karnkowska-IshikawaA,KimJI,CiuguleaI,ShinW,BennettM,KwiatowskiJ,Zakryś
B,TriemerRE(2010)Reconstructingeuglenoidevolutionaryrelationshipsusingthree
genes:nuclearSSUandLSU,andchloroplast16SrDNAsequencesandthedescriptionof
Euglenariagen.nov.(Euglenophyta).Protist161:603-19.
JasserI,Karnkowska-IshikawaA,KozłowskaE,KrólickaA,Łukomska-KowalczykM.(2010)
CompositionofpicocyanobacteriacommunityinTheGreatMazurianLakes:isolationof
phycoerythrin-richandphycocyanin-richecotypesfromthesystem-comparisonoftwo
methods.PolJMicrobiol59(1):21-31.
Karnkowska-IshikawaA,MilanowskiR,KwiatowskiJ,ZakryśB.(2010)Taxonomyofthe
Phacusoscillansanditscloserelatives-balancingmorphologicalandmolecularfeatures.J
Phycol46:172-82.
KosmalaS,Karnkowska-IshikawaA,MilanowskiR,KwiatowskiJ,ZakryśB(2009)Phylogeny
andsystematicsofEuglena(Euglenaceae)specieswithaxial,stellatechloroplastsbasedon
morphologicalandmoleculardata-newtaxa,emendeddiagnosesandepityfication.J
Phycol45:464-81.
KosmalaS,KarnkowskaA,MilanowskiR,KwiatowskiJ,ZakryśB(2005)Thephylogeneticand
taxonomicpositionofLepocinclisfuscacomb.nova(=Euglenafusca)(Euglenaceae).
Morphologicalandmolecularjustification.JPhycol41:1258-67.
BOOKCHAPTERS
Klinger CM, Karnkowska A, Herman EK, Hampl V, Dacks JB. Phylogeny and Evolution. Molecular
Parasitology–ProtozoanParasitesandtheirMolecules.JuliaWalochnikJandMichaelDuchêne(Eds).
Springer-Verlag,Wien.2016,383-408
CONFERENCESANDWORKSHOPSORGANIZER
2015
2014
Phylogenomics:newapproachestosolvingoldproblemsinalgalevolution
SymposiumOrganizer-6thEuropeanPhycologicalCongress,London,England
LocalOrganisingCommittee-Protist2014,Banff,Canada
2011
OrganizingCommitteeof54thWorkshop:EvolutionaryBiologyandRelatedTopics
2010
OrganizingCommitteeof16thEuropeanMeetingofPhDStudentsinEvolutionary
Biology(EMPSEB)
INVITEDPRESENTATIONS
11/2015
09/2014
Seminarspeaker
LifeScienceResearchCentre,UniversityofOstrava
Gainandlossofchloroplastsandmitochondriaineukaryotes
Seminarspeaker
DepartmentofCellBiologySeminar,UniversityofAlberta,Canada
Eukaryotewithnomitochondria:GenomicstudyofMonocercomonoides
demonstratesabonafideamitochondriate
09/2012
Conferencespeaker
53thmeetingoftheCzechPhycologicalSociety,Ostrava,CzechRepublic
Evolutionofintronsinnucleargenesofeuglenids
02/2012 09/2011 Kuželaseminarspeaker
ComeniusUniversityinBratislava,Slovakia
Molecularphylogenyandevolutionofautotrophiceuglenids
Conferencespeaker
52thmeetingoftheCzechPhycologicalSociety,Prague,CzechRepublic
Phylogeny,taxonomyandevolutionofautotrophiceuglenids
MANUSCRIPTREVIEWER
•
JournalofEukaryoticMicrobiology
•
JournalofPhycology
•
EuropeanJournalofProtistology
•
PolishJournalofMicrobiology
•
NovaHedwigia
•
FrontiersinEcologyandEvolution
•
CurrentGenetics
•
Protist
•
ActaSocietatisBotanicorumPoloniae
SELECTEDCONFERENCEPRESENTATIONS
1. KarnkowskaA,YubukiN,HamplV,LeanderBS(2015)Evolutionofplastid-targetedproteinsin
thesecondaryplastid-containingmixotrophiceuglenid-Rapazaviridis.6thEuropean
PhycologicalCongress,23-28August,London,England.
2.
KarnkowskaA,ZubáčováZ,VacekV,NovakL,TreitliS,BarlowL,DoležalP,RogerA,Hroudová
M,DacksJB,VlčekČ,HamplV(2014)GenomicstudyofMonocercomonoidesrevealsabonafide
amitochondriate.Protist2014,3–8August,Banff,Canada.
3.
KarnkowskaA,ZubáčováZ,VacekV,HroudováM,DacksJB,VlčekČ,HamplV(2013)Insight
intoevolutionofmetamonads:evidencefromdraftgenomeofMonocercomonoides.
ComparativeGenomicsofEukaryoticMicroorganisms:ComplexityPatternsinEukaryotic
Genomes,19–24October,SantFeliudeGuixols,Spain.
4.
KarnkowskaA,ZubáčováZ,VacekV,HroudováM,DacksJB,VlčekČ,HamplV(2013)Draft
genomeofMonocercomonoidessp.PA203(Oxymonadida).XIVInternationalCongressof
Protistology,28July-2August,Vancouver,Canada.
5.
MilanowskiR,Karnkowska-IshikawaA,BoulahdjelA,IshikawaT,ZakryśB(2012)Distribution
ofconventionalandnon-conventionalintronsintubAandtubBgenesofeuglenoids.Protist
2012,29July–3August,Oslo,Norway.
6.
Karnkowska-IshikawaA,WatzaD,BennettM,TriemerRE,ZakryśB(2011).Evolutionof
morphologyandmolecularphylogenyofgreeneuglenoids.13thCongressoftheEuropean
SocietyforEvolutionaryBiology,20-25August,Tübingen,Germany.
7.
Karnkowska-IshikawaA,WatzaD,BennettM,ZakryśB,TriemerRE(2011).Evolutionary
relationshipsofgreeneuglenoidsinferredfromtaxon-richanalysesof5genes.VIEuropean
CongressofProtistology,25-29July,Berlin,Germany.
8.
Karnkowska-IshikawaA,MilanowskiR,ZakryśB(2010)ThespeciesEuglenadeses
(Euglenaeceae)revisited:newmorphologicalandmoleculardata.AnnualMeetingofthe
PhycologicalSocietyofAmerica,10-13July,EastLansing,USA.
9.
Karnkowska-IshikawaA,LintonE,KimJ-W,ShinW,BennettM,KwiatowskiJ,ZakrysB,
TriemerRE(2009).ReconstructingeuglenoidevolutionaryrelationshipsusingnuclearSSUand
LSU,andchloroplast16SrDNAsequences.9thInternationalPhycologicalCongress.2-8August.
Tokyo,Japan.
10.
KarnkowskaA,MilanowskiR,ZakryśB(2008).Speciesconceptproblemforasexualgreen
euglenoids:conclusionsfromstudiesonthegenusPhacus.Systematics2008.7-11April.
Göttingen,Germany.
TEACHINGEXPERIENCE
2017
TeachingassistantattheWorkshoponGenomics,CeskyKrumlov,Chechia
04/2014
GuestlectureronMoleculartaxonomycourse,CharlesUniversityinPrague
2013-present
2006-2012
TeacherandcoordinatorofBiologyofmicrobialeukaryoteslectureforthefirst
yearofMscinMicrobiology,UniversityofWarsaw
TeacherofBotanycourseforfirstyearofBsc,UniversityofWarsaw
2006-2012
TeacherofFloraofPolandfieldcourseforfirstyearofBsc,UniversityofWarsaw
SUPERVISIONOFSTUDENTS
2011-2012
Bachelorstudent,MagdalenaJabłońska
Cyanotoxins-effectsonorganismsandtheiroccurrenceinPolishsurfacewaters
2012-2013
Bachelorstudent,ŁukaszŁach
Molecularandmorphologicalanalysisofnewstrainsfromthegenus
TrachelomonasisolatedfromNorth-EasternPoland
2011-2013
Masterstudent,MałgorzataKorzeniecka
Assessmentoftwomolecularmarkers(cox1and18SrDNA)aspotentialDNA
barcodesforautotrophiceuglenids(Euglenea)
WorkshopsforbiologyteachersincooperationwithWarsawCentreforSocioEducationalInnovationandTraining,Warsaw,Poland
2010-2011
Workshopsforchildren,UniversityofChildren,Warsaw,Poland
2009-present
MemberofGeneralCommitteeofPolishBiologicalOlympiad
2006-2012
Lecturesandworkshops,ScienceFestival,Warsaw,Poland
2006–2008
ExpertininternetcourseforbiologyteachersorganizedbyInternetAcademyof
Teachers(projectcoordinatedbyCenterforCitizenshipEducation),Poland
ProjectcoordinatorBiologyinXXIcentury–newlessonsscenariosandclassesin
smalltownhighschools,Poland
ProjectofficerScienceofmodernbiology–exploratoryresourcesforbiology
teachersandstudents,Poland
Laboratoryworkshopsforhighschoolstudentsandteachersincollaborationwith
CentreforInnovativeBioscienceEducation,Warsaw,Poland
OUTREACH
2011-2013
2005–2006
2005–2006
2004–2010
COURSESATTENDANCE
2016
2016 2015 2015
2013
2013
2009
2007
IntrodcutiontoR,UniversityofBritishColumbia,Canada(3days)
Programming in Python, The Unix Shell, Version Control with Git, University of
BritishColumbia,Canada(2days)
ProgrammingforBiology,ColdSpringHarbor,USA(3weeks)
WorkshoponMolecularEvolution,CeskyKrumlov,CzechRepublic(2weeks)
ProgramminginEvolutionaryBiology,Leipzig,Germany(3weeks)
WorkshoponGenomics,CeskyKrumlov,CzechRepublic(2weeks)
AppliedPhylogeneticworkshop,UCDavis,USA(10days)
PoznańSummerSchoolofBioinformatics,Poland(10days)
UNIVERSITYSERVICE
2008-2013
MemberoftheFacultyofBiologyBoard,UniversityofWarsaw
2007-2011
MemberoftheSenateCommitteeforstudentandteachingaffairs,Universityof
Warsaw
2008-2011
PhDstudentmemberoftheInstituteofBotanyboard,FacultyofSciences,
UniversityofWarsaw
2008-2011
HeadofPhDStudentsCouncilatFacultyofBiology,UniversityofWarsaw
2008-2011
Vice-presidentofPhDStudentsCouncilatUniversityofWarsaw
SCIENTIFICSOCIETYMEMBERSHIPS
•
InternationalSocietyforEvolutionaryProtistology(ISEP);2014-2016EuropeanCouncellor
•
InternationlSocietyforProtistology(ISOP),2015NominatingCommitteemember
•
BritishPhycologicalSociety(BPS)
MEDIACOVERAGE
ScienceMagazine
http://www.sciencemag.org/news/2016/05/first-eukaryotes-found-without-normal-cellular-powersupply?utm_source=newsfromscience&utm_medium=twitter&utm_campaign=noeukaryote-4261
TheScientist
http://www.the-scientist.com/?articles.view/articleNo/46077/title/Mysterious-Eukaryote-MissingMitochondria/
ScientificAmericanpodcast
http://www.scientificamerican.com/podcast/episode/microbe-breaks-the-powerhouse-rules/
npr
http://www.npr.org/sections/health-shots/2016/05/12/477691018/look-ma-no-mitochondria
WashingtonPost
https://www.washingtonpost.com/news/speaking-of-science/wp/2016/05/12/its-a-whole-new-world-ofweird-stuff-this-strange-cell-could-challenge-a-basic-rule-of-biology/
PNASblog
http://blog.pnas.org/2016/05/journal-club-rule-breaking-microbe-lacks-mitochondria/
CBCnews
http://www.cbc.ca/news/technology/mitochondria-eukaryote-1.3579238
ScienceMonitor
http://www.csmonitor.com/Science/2016/0512/Missing-mitochondria-Weird-eukaryotic-cell-lackspowerhouse?cmpid=push013s
Scitable
http://www.nature.com/scitable/blog/microbe-matters/mitochondria_an_essential_cellular_organelle
LosAngelesTimes
http://www.latimes.com/science/sciencenow/la-sci-sn-eukaryote-microbe-missing-mitochondria-20160513story.html