The 15-lipoxygenase-modified HDL3 fail to
inhibit the TNF- α-induced inflammatory
response in human endothelial cells
This information is current as
of June 17, 2017.
A. Pirillo, P. Uboldi, C. Bolego, H. Kuhn and A. L.
Catapano
J Immunol 2008; 181:8797; ;
doi: 10.4049/jimmunol.181.12.8797-a
http://www.jimmunol.org/content/181/12/8797.2
Permissions
Email Alerts
Information about subscribing to The Journal of Immunology is online at:
http://jimmunol.org/subscription
Submit copyright permission requests at:
http://www.aai.org/About/Publications/JI/copyright.html
Receive free email-alerts when new articles cite this article. Sign up at:
http://jimmunol.org/alerts
The Journal of Immunology is published twice each month by
The American Association of Immunologists, Inc.,
1451 Rockville Pike, Suite 650, Rockville, MD 20852
Copyright © 2008 by The American Association of
Immunologists All rights reserved.
Print ISSN: 0022-1767 Online ISSN: 1550-6606.
Downloaded from http://www.jimmunol.org/ by guest on June 17, 2017
Subscription
The Journal of Immunology
Corrections
Garg, A., P. F. Barnes, A. Porgador, S. Roy, S. Wu, J. S. Nanda, D. E. Griffith, W. M. Girard, N. Rawal, S. Shetty, and R. Vankayalapati.
2006. Vimentin expressed on Mycobacterium tuberculosis-infected human monocytes is involved in binding to the NKp46 receptor.
J. Immunol. 177: 6192– 6198.
Subsequent to publication of the above manuscript, an investigation by The University of Texas Health Science Center at Tyler found
that Dr. S. Roy committed scientific misconduct pertaining to this publication. Because Dr. Roy produced the data for Fig. 3 in the paper
cited above and was unable to provide the original data, the experiments were repeated and the results are shown in the new figure below.
As a result of the new figure, the following changes need to be made to the published article.
In Materials and Methods, in the paragraph titled Fluorescence microscopy, the last two sentences “Slides were examined with a Zeiss
LSM 510 confocal laser scanning microscope using a C-Apochromat ⫻63, 1.2 water immersion objective (Carl Zeiss), and the 488-nm
line of the argon laser for excitation of Oregon Green. The cells were scanned and images saved at 1024 ⫻ 1024-pixel/8-bit resolution
before importing into Adobe Photoshop (Adobe Systems) for compilation and direct printing.” should be replaced with the sentence
“Slides were examined with a fluorescence microscope (Olympus BX50).”
All panels in Fig. 3 are retracted. The correct Fig. 3 is shown below. The conclusions of the paper remain unchanged.
A
B
FIGURE 3. Surface expression of vimentin on M. tuberculosis-stimulated monocytes by fluorescence microscopy. Monocytes, either culutred alone (A)
or with gamma-irradiated M. tuberculosis (B), were fixed and incubated with FITC-conjugated anti-vimentin mAb, and slides were examined with a
fluorescence microscope.
Pirillo, A., P. Uboldi, C. Bolego, H. Kuhn, A. L. Catapano. 2008. The 15-lipoxygenase-modified HDL3 fail to inhibit the TNF-␣-induced
inflammatory response in human endothelial cells. J. Immunol. 181: 2821–2830.
The revised legend for Figure 12 should read as follows: FIGURE 12. Effect of human 15-LO-mediated modification on HDL3 functions.
A, Characterization of r15-LO- and h15-LO-modified HDL3. B, Effect of h15-LO-modified HDL3 on TNF-␣-induced adhesion molecule surface
expression. HUVEC were pretreated with HDL3 or h15-LO-HDL3 (100 ␮g/ml) for 18 h, then incubated with 10 ng/ml TNF-␣ for a further 6 h.
Cell surface expression of ICAM-1, VCAM-1, or E-selectin was evaluated by flow cytometry. Results are expressed as percentage of FITCpositive cells and are given as mean ⫾ SD from three independent experiments. C and D, HDL3 modified with h15-LO increase U937 monocyte
adhesion to endothelial cells. Cells were preincubated with HDL3 or h15-LO-HDL3 (100 ␮g/ml) for 18 h, then TNF-␣ (10 ng/ml) was added
for a further 6 h (C); alternatively, cells were incubated with HDL3 or h15-LO-HDL3 (100 ␮g/ml) for 18 h (D). Fluorescently labeled monocytes
were added to endothelial layers for 1 h, and the adhesion was measured by flow cytometry. Results are given as mean ⫾ SD from four
independent experiments performed in quadruplicate and expressed as percent vs control; ⴱ, p ⬍ 0.05; ⴱⴱ, p ⬍ 0.005; ⴱⴱⴱ, p ⬍ 0.0001.
Copyright © 2008 by The American Association of Immunologists, Inc. 0022-1767/08/$2.00
www.jimmunol.org
Downloaded from http://www.jimmunol.org/ by guest on June 17, 2017
In Results, in the paragraph titled Surface expression of vimentin on monocytes infected with M. tuberculosis H37Ra and Listeria, the
text “Infected monocytes were stained with murine anti-vimentin primary Ab and Oregon Green 488-conjugated goat anti-mouse IgG Ab
secondary Ab, and the stained cells was analyzed by fluorescence microscopy.” should read “Infected monocytes were stained with FITC
anti-vimentin Ab and the stained cells were analyzed by fluorescence microscopy.” The sentence “Cells incubated with blocking buffer
alone, or with isotype control mouse Abs, were used as controls” should be deleted.