AniGen AIV Realtime PCR Kits & Machine
AniGen AIV Realtime PCR Kits
Product
Packing
Material
Available PCR
machine
Anigen AIV
Realtime PCR Kit
48T/Kit, 96T/Kit
Taqman probe
SLAN (Anigen)
ABI
Anigen AIV H5
Realtime PCR Kit
48T/Kit, 96T/Kit
Taqman probe
SLAN (Anigen)
ABI
Anigen AIV H7
Realtime PCR Kit
48T/Kit, 96T/Kit
Taqman probe
SLAN (Anigen)
ABI
Anigen AIV H9
Realtime PCR Kit
48T/Kit, 96T/Kit
Taqman probe
SLAN (Anigen)
ABI
Anigen NDV
Realtime PCR Kit
48T/Kit, 96T/Kit
Taqman probe
SLAN (Anigen)
ABI
AniGen AIV Realtime PCR Kits Components
No.
Material
Q’ty
No.
Material
Q’ty
1
Negative
control
1vial
6
Standard
1X107
1vial
2
Positive
control
1vial
7
2X RTmaster mix
1vial
3
Standard
1X104
1vial
8
Detection
solution
1vial
4
Standard
1X105
1vial
9
RT mix
1vial
5
Standard
1X106
1vial
10
MgCl2
1vial
Charateristics of AniGen AIV Realtime PCR Kits
1. Excellent reproductive rate (Standard CV value : less 15%)
2. Quantitative Kit
3. Fast test result within 2 hours
4. Easy Test Procedures
Easy Test procedures
1. Sample preparation
1) Collect the swba smples form Fresh feces, Trachea, Intenstine
2) Dilute the swab into 600ul of RNAse free water
2) Extract the RNA using by RNeasy kit (Qiagen) or Trisol solution
(Invitrogen)
2. Mix the components in a tube and Running the Realtime PCR
2X RT-master mix
Detection solution
RNA sample
RT mix
PCR running
--- 10ul
--- 5ul
--- 4ul
--- 1ul
Evaluation 1. Reproductive Test
•Sample: :4 kinds of standard in the kit
•RT-PCR Kit : Anigen AIV realtime RT PCR Kit
•Machine : SLAN Realtime PCR machine
•Test method :It was performed by 3 times repeatedly.
•Result
Sample
SD
CV%
Std
1x104
0.16
0.4
Std
1x105
0.15
0.5
Std
1x106
0.23
0.8
Std
1x107
0.22
1.0
Evaluation 2. Comparison of detection limit with Immunoassay
•Sample: : AIV type A positive samples in allantoic fluid of chicken
embryo eggs
•Test Kit : Anigen AIV realtime RT PCR Kit , Anigen AIV Rapid Kit
•Machine : SLAN Realtime PCR machine
•Test method :It was performed by serial dilution of the AIV + samples
•Result
Samples
Rapid
Samples
RRT PCR
25 dilution
+
101 dilution
+
26 dilution
+
102 dilution
+
27 dilution
+
103 dilution
+
28 dilution
+
104 dilution
+
29 dilution
-
105 dilution
-
Evaluation 3 Comparison of sensitivity with Immunoassay
•Sample: : AIV negative normal chicken samples
•Test Kit : Anigen AIV realtime RT PCR Kit , Anigen AIV Rapid Kit
•Machine : SLAN Realtime PCR machine
•Test result
No. of
Samples
Rapid
RRT PCR
Sample1
-
-
Sample2
-
-
Sample3
-
-
Sample4
-
-
Sample5
-
-
Sample6
-
-
Sample7
-
-
Sample8
-
-
Sample9
-
-
Sample10
-
-
Sample11
-
-
Sample12
-
-
Sample13
-
-
Sample14
-
-
Result of Anigen AIV Rapid Kit
Result of realtime RT PCR Kit
Evaluation 3:
Comparison of sensitivity with Immunoassay in organs samples
•Sample: : Kidney,Trachea, Intestinal swab in 3 AIV positive samples
•Test Kit : Anigen AIV realtime RT PCR Kit , Anigen AIV Rapid Kit
•Machine : SLAN Realtime PCR machine
•Test result
Result of Anigen AIV Rapid Kit
Samples
Organ
Rapid
RRT PCR
Sample 1
Intestine
+
+
Kidney
-
+
Trachea
+/-
+
Intestine
+
+
Kidney
-
+
Trachea
+
+
Intestine
+-
+
Kidney
-
+
Trachea
+/-
+
Sample 2
Sample 3
Result of realtime RT PCR Kit
SLAN Realtime PCR machine
System overview
Total system
PCR machine
Computer system
Operation
Real-Time Fluorescence Detection System
For DNA Quantitative analysis, Genotyping
The Main Parameters of the system
Model
SLAN
Sample capacity
48 X 0.2ml
Channel
3
Exciting source
470nm
Detection filter
530nm
Fluorophores detected
Sybr Green, Fam, Tet, Joe, Rox, Tamra,
Cy3, Cy5, Tex red
Temp. range
4℃ ~ 99℃
Heating rate
3℃ /sec
Cooling rate
2℃ /sec
Temp. accuracy
± 0.5℃
Temp. Uniformity
± 0.5℃
Operating system
Win 2000
Input power
AC220V/60Hz
Energy Consumption
500VA
Dimensions(LxWxD)
500X380X260
Weight
20kg
Charateristics I of SLAN Realtime PCR Machine
Part
ANIGEN SLAN™
Advantage
Old Machine
Light source
LED
Stable light
Strong Intensity
Light concentration
Easy control
Semi permanent
Low temperature: no affect
result
Halogen lamp
Xenon lamp
Laser
Detector
PMT
(Photo Multiplier Tube)
High sensitive
High speed
Stable
CCD
Temperature
Control
system
Semiconductor
cooler/heater
High speed
Accurate Temp.
control(±0.1°C)
(4°C~ 99 °C)
No noise
No pollution
Long shelf life
Air convention
heating/cooling
system
Charateristics II of SLAN Realtime PCR Machine
1. Excellent reproductive rate
2. Easy Operation
3. Accurate result output
4. Universal supplies can be usable. Cost saving
Examples of Operation result
Examples of Operation result
Examples of Operation result
Comparison with ABI 7500 RT PCR Machine : Reproductive rate
SLAN
Sample
SD
ABI 7500
CV%
SD
CV%
Std 1x104
0.16
0.4
0.18
0.5
Std 1x105
0.15
0.5
0.21
0.6
Std 1x106
0.23
0.8
0.3
1.0
Std 1x107
0.21
0.9
0.1
0.4
Comparison with ABI 7500 RT PCR Machine : Detection limit
SLAN
ABI 7500
Dilution
CT value
Result
CT value
Result
10-1
17.92
POS
19.98
POS
10-2
22.23
POS
23.64
POS
10-3
25.59
POS
26.73
POS
10-4
28.89
POS
29.26
POS
10-5
32.62
POS
30.2
POS
10-6
35.53
NEG
34.2
NEG
ANIGEN, AIV Diagnosis Specialist
Thank You for Your Attention
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Real-Time PCR Machine SLAN