Serine/threonine-protein kinase PAK 1 recombinant protein
Catalog Number: CB10-782-55078
Related Product Names:
- PAK1 protein; PAK1; Serine/threonine-protein kinase PAK 1
- EC 2.7.11.1; p21-activated kinase 1; PAK-1; P65-PAK; Alpha-PAK; P21-activated kinase 1
- Gene Information -
Gene Name: PAK1
Gene Name Synonym: N/A
Gi #: 42794769
NCBI Acc #: NP_002567.3
Swiss Prot Acc #: Q13153
Length (aa): 545
Mol. Weight (Da): 60647
Chrom Location: N/A
Application Note: Kinase assay
Source/Host: Insect Cells
Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation
and store at recommended temperature.
Stability: 1 year at -20 degree C.
Shipping: Products may be shipped on ice pack or dry ice.
PAK1 PROTEIN TARGET DESCRIPTION:
Synonym Names for PAK1 protein: PAK1; EC 2.7.11.1; p21-activated kinase 1; PAK-1; P65-PAK; Alpha-PAK;
P21-activated kinase 1
Recombinant full-length human PAK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Function: The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related
GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and
reorganization of focal complexes. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1
and CDC2L2.
Catalytic Activity: ATP + a protein = ADP + a phosphoprotein.
Cofactor: Magnesium.
Enzyme Regulation: Activated by binding small G proteins. Binding of GTP-bound CDC42 or RAC1 to the autoregulatory
region releases monomers from the autoinhibited dimer, enables phosphorylation of Thr-423 and allows the kinase domain
to adopt an active structure. Also activated by binding to GTP-bound CDC42, independent of the phosphorylation state of
Thr-423. Phosphorylation of Thr-84 by OXSR1 inhibits this activation (By similarity).
Subunit: Homodimer in its autoinhibited state. Active as monomer. Interacts tightly with GTP-bound but not GDP-bound
CDC42/P21 and RAC1. Binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length
proteins. Component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and GIT1.
Subcellular Location: Cytoplasm. Cell membrane; cell-matrix junction; focal adhesion. Recruited to focal adhesions upon
activation.
Ptm: Autophosphorylated when activated by CDC42/p21 and RAC1.
Similarity: Contains 1 CRIB domain.
Similarity: Contains 1 protein kinase domain.
Summary:
PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling.
PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins
serve as targets targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of
biological activities. PAK1 regulates cell motility and morphology. Alternative transcripts of this gene have been found,
but their full-length natures have not yet been determined.
OMIM:
602590; gene. [NCBI / EBI]
Pathways:
KEGG pathway:
KEGG pathway:
KEGG pathway:
KEGG pathway:
KEGG pathway:
KEGG pathway:
KEGG pathway:
Axon guidance 04360
Epithelial cell signaling in Helicobacter pylori infection 05120
Focal adhesion
04510
MAPK signaling pathway
04010
Natural killer cell mediated cytotoxicity 04650
Regulation of actin cytoskeleton 04810
T cell receptor signaling pathway 04660