Reference Materials & LC Columns
1| Molecular Weight Reference Polymers
The PSS reference materials are characterized extensively
by modern analytical methods. They are available in various characterization levels: GPC, DIN Certified Standards,
MALDI validation standards, Light Scattering, Viscometry
and Certified Reference Materials. Their suitability for a
given application depends on the polydispersity, or the
level of characterization. Reference polymers come as
individual standards or assembled into kits. The kit components are carefully selected to cover given molecular
weight ranges to establish calibration curves.
1.1. Polydispersity
In principle the distinction of broad and narrow standards
is based on the PolyDispersityIndex, PDI = Mw/Mn.
Narrow Standards
Narrow polymer standards are made by living ionic
polymerization techniques or by fractionation of very
broad standards. All narrow standards have small polydispersities with sharp and slim chromatogram profile.
The Mp value is well defined and independent of the column resolution.
Tip: Mp (molar mass at the peak maximum) is the only
well defined point in the chromatogram and so far dedicated in order to construct a calibration curve.
Narrow standards are defined by average molar mass
values such as Mn (Number average molar mass) and Mw
(Weight average molar mass).
Mn and Mw are well established values to define the
molar mass distribution of a polymer.
The construction of the calibration curve in GPC is based
on the Mp values of the polymers in a calibration kit,
e.g., an ensemble of polymers of the same type but different molar masses.
Broad Standards
Broad reference materials are made by radical polymerization, polycondensation or coordinative polymerization
(polyolefins). The PDI of broad standards is usually >1.5.
For broad standards the Mp value is a function of the column resolution and therefore not defined.
Broad standards are characterized by Mw and Mn.
Modern GPC/SEC software packages allow the construction of a calibration curve by the use of Mw, Mn or the
intrinsic viscosity [η] of broad standards.
Up to 8 different broad standards can be used and
only one of the Mn, Mw, or [η] values is nescessary.
Broad standards are used:
• to validate a chromatographic system
•to determine physical constants such as the MarkHouwink constants K and a.
• to construct a calibration curve
• to proof a column missmatch.
1.2. M
ethodology for Characterization
Levels
PSS uses GPC/SEC to obtain molecular weight values,
which are subsequently confirmed by alternative
methods; e.g., Laser Light Scattering, Viscometry, MALDIToF, VPO and NMR. Each product comes with a Quality
Certificate, which provides the characterization information. The signed Quality Certificates include the testing
parameters, the molecular weight values (Mn, Mw, Mp,
PDI) and usually the chromatogram with the parameters
(conditions) at which the data was obtained.
DIN certified Standards
PSS is one of the world‘s largest manufacturers of reference polymers copolymers, and customer-designed
polymers. A great selection of polymer types in a wide
range of molar masses is produced regularly.
GPC/SEC Standards
4
1.1.1.Methodology for Characterization
Levels
PSS uses GPC/SEC to obtain molecular weight values,
which are subsequently confirmed by alternative methods; e.g., Laser Light Scattering, Viscometry, MALDI-ToF,
VPO and NMR. Each product comes with a Quality
Certificate, which provides the characterization information. The signed Quality
Certificates
include
thethat
testing
PSS Polymer standards
PSS supplies
certified
standards
parameters,
weight values
Mw, Mp,
in the stock are analyzed
via the molecular
meet the requirements
of (M
DINn,55672
GPC/SEC, yieldingPDI)
Mn, M
andchromatogram
ISO/EN 13885. Also
w, Musually
p
and
the
withthe
thePSS
parameters
and the polydispersity
(PDI = at which
Quality
all DIN and
(conditions)
theCertificates
data was fulfill
obtained.
Mw /Mn ).
ASTM requirements.
PSS documents the molecular weight
using GPC and additonal methods
such as Light Scattering, MALDI-Tof,
NMR, Viscometry or VPO.
5
1 Characterization of Polymer
Reference materials
Certified Reference
Materials CRM
Only one page available; The complete documentation will be delivered with the product.
Certified Reference Material
Classification of
Polymer Standards
BAM-P001
Standard
Poly(styrene)
(PS)
C
CH2
CH
H
CH3
n
Batch No.: ps2088
Kit
GPC organic
and aqueous
GPC
4
4
DIN certified
GPC + absolute method*
4
4
GPC + Light Scattering
+ Viscosity and physical
onstants (round robin test)
4
Polymer Standards Service USA, Inc
43 Jefferson Blvd. Suite 3
Warwick, RI 02888 USA
Phone: +1 401-780-8884
Fax: +1 401-780-8824
Email: [email protected]
www.pssgpcshop.com
Polymer Standards Service GmbH
In der Dalheimer Wiese 5
D-55120 Mainz
Tel.: +49 (0) 6131-96239-0
Fax: +49 (0) 6131-96239-11
Email: [email protected]
www.polymer.de
Certified Reference Materials (CRM) are highly characterized polymers
certified by German Federal Institute for Material Research and
Testing (BAM). Their reported molar mass result from round robin
experiments at certified laboratories. The CRM’s quality certificates
are full reports with the results of the different characterization
methods: GPC, Light Scattering and Viscometry. Further, the certificate
includes additional non-certified data, (MALDI-ToF; NMR; DSC and in
some instances Rheology data) to provide the most sophisticated set
of documentation for a particular polymer available worldwide.
Certificate of Analysis
Polymer type:
Part No:
Lot No:
Polymer type:
Part No:
Lot No:
Instrument:
Shots:
Matrix:
Monomer repeat units:
Polymer endgoups:
Side products:
3.0
2.5
PSS WinGPC Unity, Build 6727, LAB_PROD3, Instanz #1
W(log M)
1.0
0.5
2
1*10
3
1*10
GPC + MALDI + Absolute
method*
4
ReadyCal
GPC
4
Specialty polymers:
Deuterated Highly
tactic, etc.
GPC
4
Custom Request Polymerizations, Specialty Polymers
and Polymer Networks
4
Molar mass [Da]
Sample concentration
1,00 g/l
Solvent
Precolumn [8 x 50 mm]
THF
PSS SDV 5µm
Columns [analytical, each 8 x 300 mm] PSS SDV 5µm 10e3Å / 10e5Å / 10e6Å
Data Acquisition Software
PSS WinGPC
Inject volume
20 µl
Flow rate
Temperature
1,00 ml/min
23 °C
Operator
S. Fugmann
GPC/SEC - Results
Mw [Da]
1380
Mn [Da]
1320
Mp [Da]
1 370
PDI [Mw/Mn]
1,05
MALDI-ToF - Results
Mn [Da]
1 480
Calculated Mw, MALDI = Mn MALDI x (Mw/Mn) GPC
Comment:
Please note, that for some reasons (reslution, detection, matrix, counterion) the weight average molecular
weight and the number average molecular weight-values for synthetic polymers, measured by MALDI-ToF,
do not represent the true molecular weight distribution of the polymer.
Note:
Mw
= Weight average molecular weight
Mn = Number average molecular weight
Mp = Molar mass at the peak maximum
PDI = Polydispersity Index
Manufacture and control according to PSS method of analysis
Storage: Store the tightly recapped polymer standard in a dry, dark, cool area;
e.g. a refrigerator (4 °C).
Expiration date: 5 years after purchase date provided standard is stored as
described above.
Purchase date:
Polymer Standards Service GmbH
In der Dalheimer Wiese 5
D-55120 Mainz
Tel.: +49 (0) 6131-96239-0
Fax: +49 (0) 6131-96239-11
Email: [email protected]
www.polymer.de
Dr. T. Hofe
production director
Polymer Standards Service USA, Inc
43 Jefferson Blvd. Suite 3
Warwick, RI 02888 USA
Phone: +1 401-780-8884
Fax: +1 401-780-8824
Email: [email protected]
www.pssgpcshop.com
Page 2 of 2
Polymer Standards Service GmbH
In der Dalheimer Wiese 5
D-55120 Mainz
Tel.: +49 (0) 6131-96239-0
Fax: +49 (0) 6131-96239-11
Email: [email protected]
www.polymer.de
Polymer Standards Service USA, Inc
43 Jefferson Blvd. Suite 3
Warwick, RI 02888 USA
Phone: +1 401-780-8884
Fax: +1 401-780-8824
Email: [email protected]
www.pssgpcshop.com
PSS MALDI standards are suitable for the validation and comparison
of MALDI instruments. PSS provides MALDI spectra including Mw, Mn
and Mp values of selected narrow polymers, which differ in molar
mass and polymer type (polarity). Furthermore the quality certificate
comes with GPC data (Mw, Mn and Mp) and molar mass information
of one more absolute method such as NMR, Light Scattering,
Viscometry, VPO (depending on polymer type and molar mass).
The Quality Certificate includes the GPC parameters as well as conditions and values of MALDI-ToF measurements.
PSS masters a wide variety of different polymerization
techniques, thus we can synthesize almost all kinds of
homopolymers, functionalized homopolymers, copolymers and polymer networks.
Polymerization bases include but are not limited to:
•
•
•
•
•
•
Acrylate, Methacrylate
Styrene
Diene
Vinyl
Isobutylene
Ethlyleneoxide
Synthesis techniques include but are not limited to:
• Ionic polymerization (anionic, cationic)
• Radical polymerization
• Suspension polymerization
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MALDI Validation
Hewlett-Packard G2030A
250
Dithranol
104 Da
C2H5-(CH)-CH3- / H
none
MALDI-ToF - Plot
GPC/SEC - Conditions
Mw [Da]
1 550
4
Poly(styrene)
PSS-mps1.5k
ps24040ma
2.0
1.5
Detector
Shodex RI 71
GPC + Light Scattering +
Viscosity
MALDI-ToF - Conditions
3.5
1*10
LS/Visco Validation
Certificate of Analysis
Poly(styrene)
PSS-mps1.5k
ps24040ma
Molar Mass Distribution
MALDI Standards
Available as
Individual
CH3
CH3
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Light Scattering/
Viscometry Standards
Characterization Methods
1.3. Polymer Part Numbers Deviation
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PSS Light Scattering/
Viscometry Standards are suitable for the easy, fast and exact validation and data comparison of Light Scattering and Viscometry instruments. PSS provides GPC, Light Scattering and Viscometry data for
various narrow and broad distributed polymers.
The Quality Certificate includes the molar mass averages of different
methods the GPC parameters as well as conditions of Light Scattering
(LS) and/or Viscometry (Visco) measurements.
Polymer Part Numbers reflect the nominal
“Weight Average Molecular Weight” of the polymer.
Unless otherwise specified, the actual molecular weight
of a polymer shipped may vary from the nominal
mostly within a range of ±10% Molecular Weight
deviation of the listed value;
e.g. part number PSS-ps700 will have an actual Mw that
is somewhere within the range of 630 - 770 Da.
Exact values appear in the label of the vial and Quality
Certificate.
6
Reference Materials & LC Columns
1.4. Permanent and Temporary
Standards Stock
1.6. Polymer Standards Handling
PSS offers permanent and temporary stock of reference
materials. The stock of permanent materials is renewed
when a particular lot sells out whereas the temporary
part numbers will be only avalable while supplies last.
Production of temporary part numbers will be discontinued.
Temporary Standards are listed in chapter 2, Permanent
Standards are listed in chapter 3 (starting from page 9)
and in chapter 4 (Kits starting from page 13).
Look up the Temporary Standards comprehensive list
at www.polymer.de. The listing is updated regularly.
Storage of Polymer Standards
Store the standards in a dry, dark, cool area; e.g. a
­refrigerator (4oC), and tightly recapped. This keeps
excessive moisture away and increases the standard
shelf life.
1.5. Specialty Polymers
Specialty Polymers are polymers, which are not poly­
merized as “classical reference materials” regarding to
molar mass variation. The term “Specialty Polymers”
includes all different kinds of copolymers and functionalized or deuterated polymers. They are also offered on a
temporary basis. Please visit our website
www.polymer.de for an updated list.
Tip: PSS will tailor Specialty Polymers exhibiting new
topologies or novel properties, in close co-operation with
our customers. Call us for a consultation and a personal
quotation.
Copolymers
PSS offers a multitude of different copolymers. The offer
contains various topologies, compositions, architectures
as well as deuterated and partly deuterated products.
For an extensive list of our actually available copolymers
please visit our website www.polymer.de. The list is
constantly extended by new products.
WARNING: Do not store standards exposed to direct sunlight (windowsill or shelf near windows), to prevent
sample degradation over time. This condition is critical to
Poly(isoprene), Poly(butadiene) and other temperature
and/or light sensitive polymers.
Tip: Use only freshly prepared solutions and use them up
within two days. Solutions may be stored in the refrigerator temporarily.
WARNING:
Do not freeze standards in solution; freezing can destroy
the sample.
Samples Preparation for GPC Analysis
1. Prepare fresh standard solutions to assure accurate
concentrations. Precise concentration values are
required when using Light Scattering, Viscometry or
Triple Detection systems.
2. Select the appropriate clean dissolution container
(autosampler vial, bottle, flask, etc.).
3. Add an appropriate quantity of sample using an
analytical balance.
Start with the concentrations provided in table 2 as
guideline.
Select your working concentration based on detection
type and number of columns in use.
The moisture content should be determined before
starting any measurements
Dry the polymer if necessary.
Its variability depends on several parameters
(e.g. surface, molecular weight, sample preparation,
storage conditions, etc.).
4. Adjust the desired sample concentration by an exact
weight. Add the solvent volume accurately.
5. Note: If you use an internal standard for flow rate
monitoring, mix it with a fraction of mobile phase in
a separate container. Use the marked solvent to prepare your standards. Do not add it into the general
solvent bottle.
Tip: The internal standard should be monodisperse, and
elute after the separation limit.
7
2 Recommended Sample Concentration
Molecular Weight Range [Da] Concentration [g/l] or [mg/ml]
100 - 10 000
1 to 2
10 000 - 100 000
1 to 2
100 000 - 500 000
1 to 2
500 000 - 1 000 000
1 to 2
1 000 000 and above
0,5 or less
broad standards
usally 4.0 - 5.0 mg/ml
6. Close or seal the dissolution container and let sit at
room temperature for 24 hours or overnight for complete dissolution.
Tip: Let polymers of Mw < 200 000 Da, sit for 3-4 hours.
Ultra High Molecular weight standards and samples
>2 000 000 Da may take from 1-3 days to dissolve
­completely.
7. Gently swirl the vials for a homogeneous solution.
WARNING: Do not use stir bars, ultrasonic baths, microwave heating or harsh shaking as this can cause sample
degradation.
8. Standard Mixes: It is possible to dissolve up to four
standards of the same type in a given dissolution
container. If you make your mixture, the individual
standards molecular weight must be separated by
orders of magnitude to prevent coelution; e.g.
Poly(styrene): 5 000 Da, 50 000 Da, 500 000 Da.
PSS offers pre-made mixtures or kits that prevent
coelution (see ReadyCal-Kits). Note: separation will
depend on the column(s) you use.
Tip: When using a viscometer or Light Scattering detector, your standard mixture peaks must reach the baseline
between each standard injected in order for the standard
to be used in a calibration curve.
9. Inject each solution separately.
Start with the guideline below (Table 3) to develop
your optimum injection volume.
3 Recommended Injection Volume
Number of columns
(length 30 cm)
Guideline injection volume
[µl]
4 - 5 and more
3
2
1
200 to 250
100
50
20
10.Ultra High Molecular Weight Samples
(Mw > 1 000 000 Da) elute best in low concentrations, through columns of large particle (10µm,
20µm) and pore sizes.
Work at reduced flow rates (0.5 – 0.3 ml/min) and if
necessary increased injection volume. This reduces
the probability of sample degradation. Example:
Inject 100 µl of a solution of 0.1 g/l instead of 20 µl
of a solution of 0.5 g/l.
11.Use the measured Mp values of the standards to
construct a calibration curve.
1.7.
Warranty
PSS Polymer Standards
Service offers the warranty for the standards according to
specifications given in the Quality Certificate under the
following circumstances:
1. Polymers shall be stored in a cool, dry and dark
environment.
2. Do not expose the standards to UV radiation.
3. Prevent undue stress including, but not limited to,
shaking, high-pressure filtration, ultrasound, and or
microwaves, during sample dissolution and/or actual
testing.
PSS will replace any sealed standard, which was stored
according to conditions mentioned above, within one
month of its purchase date. Consumable materials are
excluded from return and exchange when seal is broken.
8
Reference Materials & LC Columns
2|Polymer Standards Temporary Stock
and Specialty Polymers
4 Temporary Stock of Organic Standards
7 Polymers with Functional End group
Polymer
Polymer
Nylon 6 broad
Nylon 6.6 broad
Molar mass range [Da]
min.
max.
15 000
40 000
32 000
110 000
Poly(carbonate) broad
4 000
60 000
Poly(ethylene) broad
50 000
100 000
1 000
75 000
Poly(isopropyl methacrylate)
14 000
Poly(n-propyl methacrylate)
Molar mass range [Da]
min.
Poly(butadiene-1.4) OH-end group
max.
10 000
90
2 000
40 000
230 000
Poly(styrene) fluorescein labeled
600
5 000 000
Poly(styrene) sulfonated labeled
5 000
18 000
100 000
Poly(styrene) with deuterium end group
2 000
70 000
15 000
60 000
Poly(styrene) without initiator end group
2 000
60 000
Poly(ortho-chlorostyrene)
100 000
300 000
Poly(styrene sulfonate-d8) lithium salt
80 000
90 000
Poly(para-methylstyrene)
3 000
800 000
Poly(isoprene-3.4)
Poly(propylene)
700
3 500
6 000
350 000
Poly(vinyl acetate) broad
17 000
300 000
Poly(vinyl chloride)
42 000
300 000
Poly(vinyl pyrrolidone) broad
14 000
1 000 000
Poly(propylene) broad
Poly(ethylene glycol) dimethylether
Poly(styrene) brominated
8 Highly Tactic Polymers
Polymer
Poly(methyl methacrylate) isotactic
Poly(methyl methacrylate) syndiotactic
5 Temporary Stock of Aqueous Standards
9 Star Polymers
Polymer
Polymer
Hydroxypropyl cellulose
Hydroxypropyl cellulose broad
Poly(2-vinylpyridinium bromide)
Poly(acrylamide) broad
Molar mass range [Da]
min.
max.
12 000
60 000
Poly(isoprene-1,4) 3-star
8 000
Molar mass range [Da]
min.
max.
30 000
150 000
900 000
Poly(styrene) 3-star
45 000
300 000
3 000
3 300 000
Poly(styrene) 8-star
120 000
230 000
3 500
9 000 000
450 000
10 Copolymers
Poly(styrene sulfonate) sodium salt broad
100 000
500 000
Excerpt of the available copolymers:
Poly(styrene) without initiator end group
2 000
60 000
Poly(styrene-b-methyl methycrylate)
Poly(styrene sulfonate-d8) lithium salt
80 000
90 000
Poly(styrene-b-n-butyl methacrylate)
Poly(styrene-b-vinylpyridine)
Poly(styrene-b-butadiene)
Poly(styrene-b-isoprene)
2.1. Specialty Polymer
Poly(styrene-b-dimethyl siloxane)
Poly(styrene-b-alpha-methylstyrene)
6 Deuterated Polymers
Polymer
Poly(butadiene-1.4-d6)
Poly(styrene-b-acrylic acid)
Molar mass range [Da]
min.
max.
23 000
190 000
Poly(carbonate-d4) broad
Poly(methyl methacrylate-b-n-butyl methacrylate)
Poly(methyl methacrylate-b-t-butyl methacrylate)
Poly(butadien-b-methacrylic acid)
Poly(isoprene-b-butadiene)
5 000
25 000
Poly(methyl-methacrylate-d8)
4 000
580 000
Poly(para-methylstyrene-d10)
Poly(styrene-b-ethylene oxide)
50 000
Poly(dimethyl siloxane-d6)
100 000
Poly(styrene-d3)
2 500
160 000
Poly(styrene-d8)
900
830 000
80 000
90 000
Poly(styrene sulfonate-d8) lithium salt
400 000
30 000
45 000
Poly(DADMAC)
Molar mass range [Da]
min.
max.
5 000
60 000
9
3|Polymer Standards Permanent Stock
3.1. Organic Standards
11 Oligo(ethylene) monodisperse
15 Poly(butadiene-1.4)
Part Number
Part Number
Molar Mass [Da]*
PDI
Weight [mg]
PSS-bdf2.1k
2 100
<1.15
1000
PSS-bdf4.7k
4 700
<1.15
1000
1000
PSS-bdf10k
10 000
<1.15
1000
1.00
1000
PSS-oe500
500
1.00
1000
340
1.00
1000
PSS-bdf21k
21 000
<1.15
1000
PSS-oe450
450
1.00
1000
PSS-bdf47k
47 000
<1.15
1000
PSS-oe500
500
1.00
1000
PSS-bdf100k
100 000
<1.15
1000
PSS-bdf210k
210 000
<1.15
1000
PSS-bdf470k
470 000
<1.15
1000
1 000 000
<1.50
1000
Molar Mass [Da]*
PDI
Weight [mg]
PSS-oe72
72
1.00
1000
PSS-oe114
114
1.00
1000
PSS-oe200
200
1.00
PSS-oe254
254
PSS-oe340
12 Poly(2-vinylpyridine)
Part Number
Molar Mass [Da]*
PDI
Weight [mg]
PSS-pvp1k
1 000
<1.50
1000
PSS-pvp2.1k
2 100
<1.50
1000
PSS-pvp4.7k
4 700
<1.15
1000
Part Number
Molar Mass [Da]*
PDI
Weight [mg]
PSS-pvp10k
10 000
<1.15
1000
PSS-pdm162
162
1.00
500
PSS-pvp21k
21 000
<1.15
1000
PSS-pdm237
237
1.00
500
PSS-pvp45k
45 000
<1.15
1000
PSS-pdm311
311
1.00
500
PSS-pvp110k
110 000
<1.15
1000
PSS-pdm1.5k
1 500
<1.50
500
PSS-pvp265k
265 000
<1.15
1000
PSS-pdm3.5k
3 500
<1.25
500
PSS-pdm10k
10 000
<1.15
500
PSS-pdm30k
30 000
<1.15
500
PSS-pdm100k
100 000
<1.15
500
Molar Mass [Da]*
PDI
Weight [mg]
13 Poly(alpha-methylstyrene)
PSS-bdf1m
16 Poly(dimethyl siloxane)
Part Number
Molar Mass [Da]*
PDI
Weight [mg]
PSS-ams1.5k
1 500
<1.50
1000
PSS-ams4k
4 000
<1.15
1000
17 Poly(ethylene)
PSS-ams8k
8 000
<1.15
1000
Part Number
PSS-ams16k
16 000
<1.15
1000
PSS-pe1.2k
1 200
<2.00
250
PSS-ams29k
29 000
<1.15
1000
PSS-pe2k
2 000
<2.00
250
PSS-ams60k
60 000
<1.15
1000
PSS-pe17k
17 000
<2.00
250
PSS-ams110k
110 000
<1.15
1000
PSS-pe21k
21 000
<2.00
250
PSS-ams230k
230 000
<1.15
1000
PSS-pe37k
37 000
<2.00
250
PSS-ams430k
430 000
<1.15
1000
PSS-pe60k
60 000
<2.00
250
PSS-ams850k
850 000
<1.15
1000
PSS-pe77k
77 000
<2.00
250
PSS-pe85k
85 000
<2.00
250
PSS-pe92k
92 000
<2.00
250
14 Poly(butadiene-1.2)
Part Number
Molar Mass [Da]*
PDI
Weight [mg]
PSS-pe110k
110 000
<2.00
250
PSS-bdt1k
1 000
<1.50
1000
PSS-pe150k
150 000
<2.00
250
PSS-bdt1.8k
1 800
<1.50
1000
PSS-pe170k
170 000
<2.00
250
PSS-bdt3.2k
3 200
<1.15
1000
PSS-pe180k
180 000
<2.00
250
PSS-bdt5.6k
5 600
<1.15
1000
PSS-bdt12k
12 000
<1.15
1000
PSS-bdt20k
20 000
<1.15
1000
15 Poly(butadiene-1.4)
Part Number
18 Poly(ethylene terephthalate)
Molar Mass [Da]*
PDI
Weight [mg]
PSS-pet3.5k
Part Number
3 500
<2.10
250
PSS-pet10k
10 000
<2.10
250
Molar Mass [Da]*
PDI
Weight [mg]
PSS-pet18k
18 000
<2.10
250
PSS-bdf110
110
1.00
1000
PSS-pet25k
25 000
<2.10
250
PSS-bdf470
470
<1.50
1000
PSS-pet35k
35 000
<2.10
250
1 000
<1.50
1000
PSS-pet40k
40 000
<2.10
250
PSS-pet50k
50 000
<2.10
250
PSS-pet75k
75 000
<2.10
250
PSS-pet120k
120 000
<2.10
250
PSS-bdf1k
This catalog represents items available on a regular
basis and therefore, deliverable immediately.
10
Reference Materials & LC Columns
22 Poly(methyl methacrylate)
19 Poly(ethyl methacrylate)
Molar Mass [Da]*
PDI
Weight [mg]
Part Number
PSS-em1.8k
1 800
<1.50
1000
PSS-mm470k
PSS-em5k
5 000
<1.15
1000
PSS-mm1m
PSS-em8k
8 000
<1.15
1000
PSS-mm2m
PSS-mm3m
Part Number
Molar Mass [Da]*
PDI
Weight [mg]
470 000
<1.15
1000
1 000 000
<1.50
1000
2 000 000
<1.50
1000
3 000 000
<1.50
1000
PSS-em45k
45 000
<1.15
1000
PSS-em145k
145 000
<1.15
1000
PSS-em165k
165 000
<1.15
1000
23 Poly(methyl methacrylate) broad)
PSS-em280k
280 000
<1.15
1000
Part Number
Molar Mass [Da]*
PDI
Weight [mg]
PSS-em300k
300 000
<1.15
1000
PSS-mmb20k
20 000
>1.50
1000
1000
PSS-mmb60k
60 000
>1.50
1000
1000
PSS-mmb100k
100 000
>1.50
1000
PSS-mmb2.2m
2 200 000
>1.50
1000
PSS-em350k
PSS-em500k
350 000
500 000
<1.15
<1.15
20 Poly(isobutylene)
Part Number
PSS-pib112
Molar Mass [Da]*
PDI
Weight [mg]
112
1.00
250
24 DIN-Poly(methyl methacrylate)
Part Number
Molar Mass [Da]*
PDI
Weight [mg]
390
<1.30
250
PSS-dmm450
450
<1.30
1000
PSS-pib1k
1 000
<1.30
250
PSS-dmm3.5k
3 500
<1.10
1000
PSS-pib2.1k
2 100
<1.30
250
PSS-dmm5k
5 000
<1.10
1000
250
PSS-dmm14k
14 000
<1.05
1000
23 000
<1.05
1000
PSS-pib390
PSS-pib4.7k
4 700
<1.30
PSS-pib10k
10 000
<1.30
250
PSS-dmm23k
PSS-pib21k
21 000
<1.30
250
PSS-dmm45k
45 000
<1.05
1000
PSS-pib47k
47 000
<1.30
250
PSS-dmm65k
65 000
<1.05
1000
PSS-pib100k
100 000
<1.30
250
PSS-dmm90k
90 000
<1.05
1000
250
PSS-dmm170k
170 000
<1.05
1000
250
PSS-dmm350k
350 000
<1.05
1000
250
PSS-dmm600k
600 000
<1.05
1000
PSS-dmm850k
850 000
<1.10
1000
PSS-pib210k
PSS-pib470k
PSS-pib700k
210 000
470 000
700 000
<1.30
<1.30
<1.30
21 Poly(isoprene-1.4)
Part Number
Molar Mass [Da]*
PDI
Weight [mg]
25 CRM-Poly(methyl methacrylate)
800
<1.50
1000
Part Number
Molar Mass [Da]*
PDI
Weight [mg]
PSS-pio1k
1 000
<1.50
1000
PSS-cmm350k
365 900
1.25
1000
PSS-pio2.1k
2 100
<1.15
1000
PSS-pio4.7k
4 700
<1.15
1000
PSS-pio10k
10 000
<1.15
1000
PSS-pio21k
21 000
<1.15
1000
PSS-pio47k
47 000
<1.15
1000
PSS-pio100k
100 000
<1.15
1000
PSS-pio210k
210 000
<1.15
1000
PSS-pio470k
470 000
<1.15
1000
1 000 000
<1.50
1000
PSS-pio800
PSS-pio1m
22 Poly(methyl methacrylate)
Part Number
Molar Mass [Da]*
PDI
Weight [mg]
PSS-mm102
102
1.00
1000
PSS-mm202
202
1.00
1000
PSS-mm600
600
<1.50
1000
PSS-mm1k
1 000
<1.50
1000
PSS-mm2.1k
2 100
<1.15
1000
PSS-mm4.7k
4 700
<1.15
1000
PSS-mm10k
10 000
<1.15
1000
PSS-mm21k
21 000
<1.15
1000
PSS-mm47k
47 000
<1.15
1000
PSS-mm100k
100 000
<1.15
1000
PSS-mm210k
210 000
<1.15
1000
26 CRM-Poly(methyl methacrylate)broad
Part Number
Molar Mass [Da]*
PDI
Weight [mg]
PSS-cmmb100k
101 100
2.15
1000
PSS-cmmb350k
366 400
2.23
1000
Molar Mass [Da]*
PDI
Weight [mg]
PSS-cpla90k
92 400
1.68
1000
PSS-cpla230k
249 400
1.98
1000
27 CRM-Poly(lactide)
Part Number
28 Poly(n-butyl methacrylate)
Molar Mass [Da]*
PDI
Weight [mg]
PSS-nb1k
Part Number
1 000
<1.50
1000
PSS-nb2.8k
2 800
<1.15
1000
PSS-nb5.5k
5 500
<1.15
1000
PSS-nb12k
12 000
<1.15
1000
PSS-nb20k
20 000
<1.15
1000
PSS-nb47k
47 000
<1.15
1000
PSS-nb100k
100 000
<1.15
1000
PSS-nb210k
210 000
<1.15
1000
PSS-nb470k
470 000
<1.15
1000
PSS-nb700k
700 000
<1.15
1000
11
29 Poly(propylene glycol)
Part Number
32 DIN-Poly(styrene)
Molar Mass [Da]*
PDI
Weight [mg]
Molar Mass [Da]*
PDI
Weight [mg]
PSS-ppg76
76
1.00
1000
PSS-dps162
162
1.00
1000
PSS-ppg192
192
1.00
1000
PSS-dps700
700
<1.20
1000
PSS-ppg450
450
<1.25
1000
PSS-dps1.4k
1 400
<1.20
1000
PSS-ppg790
Part Number
790
<1.25
1000
PSS-dps3.2k
3 200
<1.05
1000
PSS-ppg1k
1 000
<1.25
1000
PSS-dps9k
9 000
<1.05
1000
PSS-ppg3k
3 000
<1.25
1000
PSS-dps18k
18 000
<1.05
1000
PSS-ppg5k
5 000
<1.25
1000
PSS-dps32k
32 000
<1.05
1000
PSS-dps100k
100 000
<1.05
1000
30 Poly(styrene)
PSS-dps250k
280 000
<1.05
1000
Molar Mass [Da]*
PDI
Weight [mg]
PSS-dps560k
560 000
<1.05
1000
PSS-ps162
162
1.00
1000
PSS-dps800k
800 000
<1.05
1000
PSS-ps266
266
1.00
1000
PSS-dps1.8m
1 800 000
<1.20
1000
PSS-ps370
370
1.00
1000
Molar Mass [Da]*
PDI
Weight [mg]
79 600
1.08
1000
Part Number
PSS-ps560
33 CRM-Poly(styrene)
560
<1.50
1000
PSS-ps1k
1 000
<1.50
1000
Part Number
PSS-ps1.8k
1 800
<1.50
1000
PSS-cps80k
PSS-ps3.2k
3 200
<1.15
1000
PSS-ps5.6k
5 600
<1.15
1000
34 CRM-Poly(styrene) broad
PSS-ps10k
10 000
<1.15
1000
Part Number
Molar Mass [Da]*
PDI
Weight [mg]
PSS-ps18k
18 000
<1.15
1000
PSS-cpsb180k
181 200
2.26
1000
PSS-ps33k
33 000
<1.15
1000
PSS-cpsb330k
311 800
2.25
1000
PSS-ps56k
56 000
<1.15
1000
PSS-ps100k
100 000
<1.15
1000
PSS-ps180k
180 000
<1.15
1000
PSS-ps320k
320 000
<1.15
1000
PSS-ps560k
560 000
<1.15
1000
PSS-ps1m
1 000 000
<1.50
1000
PSS-ps1.8
1 800 000
<1.50
1000
PSS-ps3.2m
3 200 000
<1.50
1000
PSS-ps5m
5 000 000
<1.50
1000
PSS-tba1m
PSS-ps10m
10 000 000
<1.50
1000
PSS-ps20m
20 000 000
<1.50
1000
36 Poly(t-butyl methacrylate)
31 Poly(styrene) broad
Part Number
Molar Mass [Da]*
PDI
Weight [mg]
PSS-psb45k
45 000
>1.50
1000
PSS-psb100k
100 000
>1.50
1000
PSS-psb250k
250 000
>1.50
1000
PSS-psb450k
450 000
>1.50
1000
35 Poly(t-butyl acrylate)
Molar Mass [Da]*
PDI
Weight [mg]
PSS-tba27k
Part Number
27 000
<1.25
1000
PSS-tba47k
47 000
<1.25
1000
PSS-tba100k
100 000
<1.25
1000
PSS-tba210k
210 000
<1.25
1000
PSS-tba470k
470 000
<1.25
1000
1 000 000
<1.50
1000
Part Number
Molar Mass [Da]*
PDI
Weight [mg]
PSS-tbma1.5k
1 500
<1.50
1000
PSS-tbma2.1k
2 100
<1.15
1000
PSS-tbma4.7k
4 700
<1.15
1000
PSS-tbma10k
10 000
<1.15
1000
PSS-tbma21k
21 000
<1.15
1000
PSS-tbma47k
47 000
<1.15
1000
PSS-tbma100k
100 000
<1.15
1000
PSS-tbma210k
210 000
<1.15
1000
470 000
<1.15
1000
1 000 000
<1.50
1000
PSS-tbma470k
PSS-tbma1m
*) For most part numbers with a PDI ≠ 1 the molar mass produced will be
within ±10% of the listed nominal molar mass (15-20% for some exceptions). e.g. part number PSS-ps700 will have an actual molar mass that is
somewhere within the range of 630 - 770 Da. Exact values appear in the
label of the vial and Quality Certificate.
12
Reference Materials & LC Columns
3.2. Aqueous Standards
37 Hydroxyethyl starch
Part Number
40 DIN-Dextran
Molar Mass [Da]*
PDI
Weight [mg]
Part Number
Molar Mass [Da]*
PDI
Weight [mg]
PSS-hes24k
24 000
<2.50
250
PSS-ddxt25k
25 000
<1.50
1000
PSS-hes50k
50 000
<2.50
250
PSS-ddxt50k
50 000
<1.50
1000
PSS-hes140k
140 000
<2.50
250
PSS-ddxt80k
80 000
<1.50
1000
PSS-hes350k
350 000
<2.50
250
PSS-ddxt150k
150 000
<1.50
1000
PSS-hes800k
800 000
<2.50
250
PSS-ddxt270k
270 000
<2.00
1000
PSS-hes1.4m
1 400 000
<2.50
250
PSS-ddxt410k
410 000
<2.00
1000
PSS-hes2m
2 000 000
<2.50
250
PSS-ddxt3m
3 000 000
>1.70
1000
Molar Mass [Da]*
PDI
Weight [mg]
PSS-dxt180
180
1.00
1000
PSS-dxt342
342
1.00
1000
PSS-dxt504
504
1.00
PSS-dxt1.3k
1 300
PSS-dxt5k
38 Dextran
Part Number
41Poly(acrylic acid) sodium salt
Molar Mass [Da]*
PDI
Weight [mg]
PSS-paa2k
2 000
<2.00
250
PSS-paa4k
4 000
<2.00
250
1000
PSS-paa8k
8 000
<2.00
250
<1.50
1000
PSS-paa18k
18 000
<2.00
250
5 000
<2.00
1000
PSS-paa35k
35 000
<2.00
250
PSS-dxt12k
12 000
<1.50
1000
PSS-paa130k
130 000
<2.00
250
PSS-dxt25k
25 000
<1.50
1000
PSS-paa245k
245 000
<2.00
250
PSS-dxt50k
50 000
<1.50
1000
PSS-paa585k
585 000
<2.00
250
PSS-dxt80k
80 000
<1.50
1000
PSS-paa1m
1 000 000
<2.00
250
PSS-dxt150k
150 000
<1.50
1000
PSS-paa1.3m
1 300 000
<2.00
250
PSS-dxt270k
270 000
<2.00
1000
PSS-dxt410k
410 000
<2.00
1000
42 Poly(ethylene glycol)
PSS-dxt670k
670 000
<2.50
1000
Part Number
Molar Mass [Da]*
PDI
Weight [mg]
PSS-peg106
106
1.00
1000
PSS-peg194
194
1.00
1000
39 Dextran broad/branched
Part Number
Part Number
Molar Mass [Da]*
PDI
Weight [mg]
PSS-peg232
232
1.00
1000
PSS-dxtb4k
4 000
>1.50
1000
PSS-peg330
330
<1.25
1000
PSS-dxtb10k
10 000
>1.50
1000
PSS-peg400
400
<1.25
1000
PSS-dxtb40k
40 000
>1.50
1000
PSS-peg600
600
<1.25
1000
PSS-dxtb70k
70 000
>1.50
1000
PSS-peg1k
1 000
<1.25
1000
PSS-dxtb500k
500 000**
>1.70
1000
PSS-peg1.5k
1 500
<1.25
1000
PSS-dxtb1.5m
1 500 000**
>1.70
1000
PSS-peg2k
2 000
<1.25
1000
PSS-dxtb3m
3 000 000**
>1.70
1000
PSS-peg3k
3 000
<1.25
1000
PSS-dxtb17m
17 000 000**
>1.70
1000
PSS-peg4k
4 000
<1.25
1000
PSS-peg6k
6 000
<1.25
1000
PSS-peg10k
10 000
<1.25
1000
40 DIN-Dextran
Part Number
Molar Mass [Da]*
PDI
Weight [mg]
PSS-peg12k
12 000
<1.25
1000
PSS-ddxt180
180
1.00
1000
PSS-peg18k
18 000
<1.25
1000
PSS-ddxt342
342
1.00
1000
PSS-peg26k
26 000
<1.25
1000
PSS-ddxt1.3k
1 300
<1.50
1000
PSS-peg42k
42 000
<1.30
1000
PSS-ddxt5k
5 000
<2.00
1000
PSS-ddxt12k
12 000
<1.50
1000
13
43 DIN-Poly(ethylene glycol)
47 Poly(styrene sulfonate) sodium salt
Part Number
Molar Mass [Da]*
PDI
Weight [mg]
Molar Mass [Da]*
PDI
Weight [mg]
PSS-dpeg106
106
1.00
1000
PSS-pss1k
1 000
<1.20
500
PSS-dpeg194
194
1.00
1000
PSS-pss3.4k
3 400
<1.20
500
PSS-dpeg400
Part Number
400
<1.25
1000
PSS-pss6k
6 000
<1.20
500
PSS-dpeg1k
1 000
<1.25
1000
PSS-pss15k
15 000
<1.20
500
PSS-dpeg2k
2 000
<1.25
1000
PSS-pss30k
30 000
<1.20
500
PSS-dpeg3k
3 000
<1.25
1000
PSS-pss67k
67 000
<1.20
500
PSS-dpeg6k
6 000
<1.25
1000
PSS-pss140k
140 000
<1.20
500
PSS-dpeg12k
12 000
<1.25
1000
PSS-pss280k
280 000
<1.20
500
PSS-dpeg26k
26 000
<1.25
1000
PSS-pss600k
600 000
<1.20
500
PSS-dpeg42k
42 000
<1.30
1000
PSS-pss1m
1 000 000
<1.20
500
PSS-pss2m
2 000 000
<1.20
500
44 Poly(ethylene oxide)
Part Number
48 Poly(vinyl alcohol) broad
Molar Mass [Da]*
PDI
Weight [mg]
PSS-peo42k
42 000
<1.30
1000
Part Number
Molar Mass [Da]*
PDI
Weight [mg]
PSS-peo110k
110 000
<1.35
1000
PSS-pvo5k
5 000
<2.50
1000
12 000
<3.50
1000
PSS-peo220k
PSS-peo500k
PSS-peo1m
220 000
<1.35
1000
PSS-pvo12k
500 000
<1.50
1000
PSS-pvo30k
30 000
<2.50
1000
1 000 000
<1.35
1000
PSS-pvo40k
40 000
<2.50
1000
PSS-pvo75k
75 000
<2.50
1000
PSS-pvo100k
100 000
<2.50
1000
45 CRM-Poly(ethylene oxide)
Part Number
Molar Mass [Da]*
PDI
Weight [mg]
PSS-pvo120k
120 000
<2.50
1000
PSS-cpeo6k
6 200
1.06
1000
PSS-pvo160k
160 000
<2.50
1000
PSS-cpeo11k
11 350
1.11
1000
46 Poly(methacrylic acid) sodium salt
49 DIN-Pullulan
Part Number
Molar Mass [Da]*
PDI
Weight [mg]
Part Number
Molar Mass [Da]*
PDI
Weight [mg]
PSS-dpul342
342
1.00
100
PSS-pma1.2k
1 200
<1.20
500
PSS-dpul1.3k
1 300
<1.30
100
PSS-pma3.5k
3 500
<1.20
500
PSS-dpul6k
6 000
<1.20
100
PSS-pma7.6k
7 600
<1.20
500
PSS-dpul12k
12 000
<1.20
100
PSS-pma18k
18 000
<1.20
500
PSS-dpul22k
22 000
<1.20
100
PSS-pma36k
36 000
<1.20
500
PSS-dpul50k
50 000
<1.20
100
PSS-pma76k
76 000
<1.20
500
PSS-dpul110k
110 000
<1.20
100
PSS-pma160k
160 000
<1.20
500
PSS-dpul200k
200 000
<1.20
100
PSS-pma340k
340 000
<1.20
500
PSS-dpul400k
400 000
<1.20
100
PSS-pma500k
500 000
<1.20
500
PSS-dpul800k
800 000
<1.20
100
PSS-dpul1.6m
1600 000
<1.20
100
PSS-dpul2.5m
2 500 000
<1.50
100
*)For most part numbers with a PDI ≠ 1 the molar mass produced will be within ±10% of the listed nominal molar mass (15-20% for some exceptions). e.g. part number PSS-ps700 will have an actual molar mass that is somewhere within the range of 630 - 770 Da. Exact values appear in
the label of the vial and Quality Certificate.
**) Mw based on light scattering
14
Reference Materials & LC Columns
4|Assembled Kits of Molecular Weight Standards
4.1. GPC/SEC Calibration Kits
51 Kit Poly(2-vinylpyridine)
PSS-pvpkit
8 x 1000 mg
Mw Part [Da]*
PDI
1 000
< 1.50
2 100
< 1.50
4 700
< 1.15
10 000
< 1.15
21 000
< 1.15
45 000
< 1.15
110 000
< 1.15
265 000
< 1.15
52 Kit Poly(alpha-methylstyrene)
PSS-amskit
10 x 1000 mg
A calibration kit consists of 8 to 12 well-characterized
standards of one polymer type. The kits include a calibration report using the individual standards, as well as the
analytical conditions to create the calibration curve.
A Quality Certificate accompanies each standard with all
pertinent analytical parameters and molar mass distribution via GPC.
The composition of a kit may change.
Please visit www.polymer.de for an updated list.
4.2. Organic Standards
50 Kit Oligo(ethylene) monodisperse
PSS-oekit
8 x 500 mg
Mw Part [Da]*
PDI
72
1.00
86
1.00
114
1.00
170
1.00
225
1.00
280
1.00
390
1.00
500
1.00
Mw Part [Da]*
PDI
1 500
< 1.50
4 000
< 1.15
8 000
< 1.15
16 000
< 1.15
29 000
< 1.15
52 Kit Poly(alpha-methylstyrene)
PSS-amskit
10 x 1000 mg Mw Part [Da]*
PDI
60 000
< 1.15
110 000
< 1.15
230 000
< 1.15
430 000
< 1.15
850 000
< 1.15
53 Kit Poly(butadiene-1.4)
PSS-bdfkit
10 x 1000 mg
Mw Part [Da]*
PDI
1 000
< 1.50
2 100
< 1.50
4 700
< 1.15
10 000
< 1.15
18 000
< 1.15
35 000
< 1.15
100 000
< 1.15
170 000
< 1.15
500 000
< 1.15
1 000 000
< 1.50
15
54 Kit Poly(dimethyl siloxane)
PSS-pdmkit
8 x 500 mg
Mw Part [Da]*
PDI
58 Kit Poly(L-lactide) low
PSS-plakit
8 x 200 mg
Mw Part [Da]*
PDI
311
1.00
150
1.00
2 000
< 1.50
700
< 1.15
6 000
< 1.25
1 500
< 1.15
15 000
< 1.15
2 500
< 1.15
30 000
< 1.15
10 000
< 1.15
60 000
< 1.15
20 000
< 1.15
150 000
< 1.15
28 000
< 1.20
200 000
< 1.15
40 000
< 1,40
55 Kit Poly(ethylene)
PSS-pekit
Mw Part [Da]*
59 Kit Poly(L-lactide) high
10 x 250 mg
PDI
340
1.00
500
1.00
1 200
< 2.00
2 000
< 2.00
17 000
< 2.00
37 000
< 2.00
60 000
< 2.00
85 000
< 2.00
110 000
< 2.00
180 000
< 2.00
56 Kit Poly(isobutylene)
PSS-pibkit
10 x 250 mg
PSS-plakith
8 x 200 mg + 1
(broad) x 500 mg
Mw Part [Da]*
PDI
150
1.00
700
< 1.15
1 500
< 1.15
2 500
< 1.15
10 000
< 1.15
20 000
< 1.15
28 000
< 1.20
40 000
< 1,40
200 000
> 1.50
60 Kit Poly(methyl methacrylate) high
PSS-mmkith
12 x 500 mg
Mw Part [Da]*
PDI
Mw Part [Da]*
350
< 1.30
102
1.00
1 000
< 1.30
800
< 1.50
2 100
< 1.30
2 500
< 1.15
4 700
< 1.30
6 500
< 1.15
10 000
< 1.30
12 500
< 1.15
29 000
< 1.30
23 000
< 1.15
47 000
< 1.30
40 000
< 1.15
150 000
< 1.30
85 000
< 1.15
300 000
< 1.30
210 000
< 1.15
700 000
< 1.30
380 000
< 1.15
615 000
< 1.15
1 000 000
< 1.50
57 Kit Poly(isoprene-1.4)
PSS-piokit
10 x 1000 mg
Mw Part [Da]*
PDI
1 000
< 1.50
2 100
< 1.15
4 700
< 1.15
10 000
< 1.15
21 000
< 1.15
47 000
< 1.15
100 000
< 1.15
210 000
< 1.15
470 000
< 1.15
1 000 000
< 1.15
PDI
61 Kit Poly(methyl methacrylate) low
PSS-mmkitl
8 x 500 mg
Mw Part [Da]*
PDI
102
1.00
800
< 1.50
2 100
< 1.15
3 000
< 1.15
4 700
< 1.15
6 500
< 1.15
8 000
< 1.15
17 000
< 1.15
16
Reference Materials & LC Columns
62 Kit Poly(n-butyl methacrylate)
PSS-nbkit
9 x 1000 mg
66 Kit Poly(t-butyl methacrylate)
PSS-tbmakit
10 x 1000 mg
Mw Part [Da]*
PDI
Mw Part [Da]*
PDI
1 500
< 1.50
1 500
< 1.50
3 500
< 1.15
2 100
< 1.15
7 500
< 1.15
4 700
< 1.15
20 000
< 1.15
10 000
< 1.15
47 000
< 1.15
16 000
< 1.15
100 000
< 1.15
40 000
< 1.15
210 000
< 1.15
100 000
< 1.15
470 000
< 1.15
300 000
< 1.15
700 000
< 1.15
600 000
< 1.15
1 000 000
< 1.50
63 Kit Poly(propylene glycol)
PSS-ppgkit
7 x 1000 mg
Mw Part [Da]*
76
PDI
1.00
4.3. Aqueous Standards
192
1.00
450
< 1.25
790
< 1.25
1 000
< 1.25
3 000
< 1.25
180
1.00
5 000
< 1.25
342
1.00
64 Kit Poly(styrene) high
PSS-pskith
12 x 1000 mg
67 Kit Dextran
PSS-dxtkit
Mw Part [Da]*
10 x 500 mg
PDI
1 300
< 2.00
5 000
< 2.00
12 000
< 1.50
Mw Part [Da]*
PDI
25 000
< 1.50
560
< 1.50
50 000
< 1.50
1 200
< 1.50
150 000
< 1.50
3 200
< 1.15
270 000
< 2.00
8 000
< 1.15
410 000
< 2.00
18 000
< 1.15
33 000
< 1.15
62 000
< 1.15
120 000
< 1.15
280 000
< 1.15
560 000
< 1.15
1 200 000
< 1.15
2 500 000
< 1.50
65 Kit Poly(styrene) low
PSS-pskitl
8 x 1000 mg
Mw Part [Da]*
PDI
162
1.00
560
< 1.50
1 200
< 1.50
2 500
< 1.15
3 200
< 1.15
4 500
< 1.15
8 000
< 1.15
18 000
< 1.15
68 Kit Hydroxyethyl starch
PSS-heskit
7 x 250 mg
Mw Part [Da]*
PDI
24 000
< 2.50
50 000
< 2.50
140 000
< 2.50
350 000
< 2.50
800 000
< 2.50
1 400 000
< 2.50
2 000 000
< 2.50
17
69 Kit Poly(acrylic acid) sodium salt
PSS-paakit
10 x 250 mg
72 Kit Poly(methacrylic acid) sodium salt
PSS-pmakit
8 x 500 mg
Mw Part [Da]*
PDI
Mw Part [Da]*
PDI
2 000
< 2.00
1 200
< 1.20
4 000
< 2.00
3 500
< 1.20
8 000
< 2.00
7 600
< 1.20
18 000
< 2.00
36 000
< 1.20
35 000
< 2.00
76 000
< 1.20
130 000
< 2.00
160 000
< 1.20
245 000
< 2.00
340 000
< 1.20
500 000
< 1.20
585 000
< 2.00
1 000 000
< 2.00
1 300 000
< 2.00
70 Kit Poly(ethylene glycol)
PSS-pegkit
10 x 500 mg
Mw Part [Da]*
PDI
73 Kit Poly(styrene sulfonate) sodium salt
PSS-psskit
10 x 500 mg
Mw Part [Da]*
PDI
1 000
< 1.20
3 400
< 1.20
106
1.00
6 000
< 1.20
194
1.00
15 000
< 1.20
400
< 1.25
30 000
< 1.20
1 000
< 1.25
67 000
< 1.20
2 000
< 1.25
140 000
< 1.20
3 000
< 1.25
280 000
< 1.20
6 000
< 1.25
600 000
< 1.20
12 000
< 1.25
1 000 000
< 1.20
26 000
< 1.25
42 000
< 1.30
74 Kit Pullulan
PSS-pulkit
10 x 100 mg
71 Kit Poly(ethylene oxide)
PSS-peokit
8 x 500 mg Mw Part [Da]*
PDI
Mw Part [Da]*
PDI
342
1.00
1 300
< 1.30
25 000
< 1.35
6 000
< 1.20
42 000
< 1.35
10 000
< 1.20
60 000
< 1.35
22 000
< 1.20
110 000
< 1.35
50 000
< 1.20
180 000
< 1.35
110 000
< 1.20
270 000
< 1.35
200 000
< 1.20
500 000
< 1.50
400 000
< 1.20
1 000 000
< 1.50
800 000
< 1.30
*)For all parts with a PDI ≠ 1 the molar mass produced will be within ±10% of the listed nominal molar mass
(15-20% for broad standards). e.g. part number PSS-ps700 will have an actual molar mass that is somewhere within the range of 630 - 770 Da.
Exact values appear in the label of the vial and Quality Certificate.
18
Reference Materials & LC Columns
4.4. ReadyCal Kits
76 ReadyCal-Kit Poly(styrene) high
PSS-pskitr4h
3 x 10 Vials-4.0ml
PSS-pskitr1h
3 x 10 Vials-1.5ml
PSS ReadyCal Standards are polymer cocktails pre
weighted into auto-sampler vials. Each vial has three or
four polymers of the same type with different molar
masses. Carefully selected molar masses are placed into
each vial so that the polymer traces are baseline separated (if a proper column combination is used)
A ReadyCal Kit allows you to prepare fast and repro­
ducible a 8 - 12 point calibration curve without the mess
of weighing samples. Just add solvent directly into the
auto sampler vial, let it stand for two hours, shake
gently and load into your auto sampler for analysis.
Each kit contains 30 autosampler vials (for at least 10
calibration curves) that are color coded for your
convenience.
Available in 1.5ml and 4.0ml vials.
75 ReadyCal-Kit Poly(methyl methacrylate)
PSS-mmkitr4
3 x 10 Vials-4.0ml
PSS-mmkitr1
3 x 10 Vials-1.5ml
Mw Part [Da]*
PDI
1 500
< 1.50
3 200
< 1.15
8 000
< 1.15
18 000
< 1.15
33 000
< 1.15
62 000
< 1.15
120 000
< 1.15
238 000
< 1.15
560 000
< 1.15
1 200 000
< 1.50
2 500 000
< 1.50
6 500 000
< 1.50
77 ReadyCal-Kit Poly(styrene) low
PSS-pskitr4l
3 x 10 Vials-4.0ml
PSS-pskitr1l
3 x 10 Vials-1.5ml
Mw Part [Da]*
PDI
266
1.00
560
< 1.50
1 200
< 1.50
2 500
< 1.50
3 200
< 1.15
4 500
< 1.15
8 000
< 1.15
18 000
< 1.15
33 000
< 1.15
62 000
< 1.15
78 ReadyCal-Kit Poly(styrene)
PSS-pskitr4
3 x 10 Vials-4.0ml
PSS-pskitr1
3 x 10 Vials-1.5ml
Mw Part [Da]*
PDI
560
< 1.50
1 200
< 1.50
3 200
< 1.15
< 1.50
8 000
< 1.15
< 1.15
18 000
< 1.15
< 1.15
33 000
< 1.15
12 500
< 1.15
62 000
< 1.15
23 000
< 1.15
120 000
< 1.15
40 000
< 1.15
280 000
< 1.15
< 1.15
560 000
< 1.15
< 1.15
1 200 000
< 1.50
380 000
< 1.15
2 500 000
< 1.50
Mw Part [Da]*
PDI
800
2 500
6 500
85 000
210 000
615 000
< 1.15
1 000 000
< 1.15
1 500 000
< 1.50
19
79 ReadyCal-Kit PEO/PEG
PSS-peokitr4
3 x 10 Vials-4.0ml
PSS-peokitr1
3 x 10 Vials-1.5ml
Mw Part [Da]*
PDI
232
1.00
600
< 1.25
2 000
< 1.25
6 000
< 1.25
18 000
< 1.25
42 000
< 1.30
110 000
< 1.35
270 000
< 1.35
500 000
< 1.50
1 000 000
< 1.50
80 ReadyCal-Kit Poly(ethylene glycol)
PSS-pegkitr1
3 x 10 Vials-1.5ml
Mw Part [Da]*
PDI
232
1.00
330
< 1.25
600
< 1.25
1 000
< 1.25
1 500
< 1.25
3 000
< 1.25
4 000
< 1.25
6 000
< 1.25
12 000
< 1.25
18 000
< 1.25
26 000
< 1.25
42 000
< 1.30
82 DIN-Kit Poly(styrene)
PSS-pskitd
12 x 1000 mg
Mw Part [Da]*
1.00
700
< 1.20
1 400
< 1.20
3 200
< 1.05
9 000
< 1.05
18 000
< 1.20
32 000
< 1.05
100 000
< 1.05
250 000
< 1.05
560 000
< 1.05
800 000
< 1.05
1 800 000
< 1.20
83 DIN-Kit Dextran
PSS-dxtkitd
10 x 500 mg
Mw Part [Da]*
PDI
180
1.00
342
1.00
1 300
< 1.50
5 000
< 2.00
12 000
< 1.50
25 000
< 1.50
50 000
< 1.50
150 000
< 1.50
270 000
< 2.00
410 000
< 2.00
84 DIN-Kit Poly(ethylene glycol)
PSS-pegkitd
10 x 500 mg
Mw Part [Da]*
4.5. DIN Kits
106
81 DIN-Kit Poly(methyl methacrylate)
PSS-mmkitd
12 x 500 mg
PDI
162
PDI
1.00
194
1.00
400
< 1.25
1 000
< 1.25
2 000
< 1.25
Mw Part [Da]*
PDI
3 000
< 1.25
450
< 1.30
6 000
< 1.25
3 500
< 1.10
12 000
< 1.25
5 000
< 1.10
26 000
< 1.25
14 000
< 1.05
23 000
< 1.05
42 000
< 1.30
45 000
< 1.05
65 000
< 1.05
90 000
< 1.05
170 000
< 1.05
350 000
< 1.05
600 000
< 1.05
850 000
< 1.10
20
Reference Materials & LC Columns
4.6. MALDI Validation Kits
4.7. Light Scattering/Viscometry
Validation Kit
PSS provides Polymer Standard Kits that will help you to
check, calibrate and validate a Matrix-Assisted-LaserDesorption-Ionization-Time-of-Flight measurement (MALDIToF) instrument. Standards with different molecular
weight ranges and different polarities are included. The
different molecular weights allow you to determine the
resolution of the instrument as a function of the molecular
weight, whereas different polymer polarities help you
determine the compatibility of your Matrix and polymer.
The kit is useful to check the instrument calibration and
the delay volume between the concentration detector
and the molar mass detector.
85 MALDI-Kit Poly(methyl methacrylate)
PSS-mmkitm
6 x 500 mg
This kit includes a mixture of well defined Light
Scattering (LS) and/or Viscometry (Visco) reference
materials (narrow and broad distributed) with the
relevant Light Scattering and/or Viscometry data. The
validation of your Light Scattering or Viscometry instrument is simple, fast and reliable.
89 LS-Visco-Kit Poly(methyl methacrylate)
PSS-mmkitv
4 x 500 mg
Mw Part [Da]*
PDI
Mw Part [Da]*
PDI
450
< 1.50
8 000
< 1.15
2 500
< 1.15
100 000
> 1.50
5 000
< 1.15
160 000
< 1.05
10 000
< 1.15
850 000
< 1.15
23 000
< 1.15
60 000
< 1.15
86 MALDI-Kit Poly(styrene)
PSS-pskitm
6 x 500 mg
90 LS-Visco-Kit Poly(styrene)
PSS-pskitv
4 x 500 mg
Mw Part [Da]*
PDI
9 000
< 1.05
Mw Part [Da]*
PDI
120 000
< 1.05
700
< 1.50
250 000
> 2.50
1 200
< 1.50
560 000
< 1.05
5 000
< 1.15
9 000
< 1.15
34 000
< 1.15
65 000
< 1.15
91 LS-Visco-Kit Dextran
PSS-dxtkitv
4 x 500 mg
Mw Part [Da]*
87 MALDI-Kit Poly(ethylene glycol)
PSS-pegkitm
6 x 500 mg
Mw Part [Da]*
PDI
400
< 1.25
1 000
< 1.25
3 000
< 1.25
6 000
< 1.25
12 000
< 1.25
26 000
< 1.25
PDI
12 000
< 1.50
50 000
< 1.50
70 000
> 1.50
410 000
< 2.00
88 MALDI mixed-Kit PS, PMMA, PDMS, PEG, PSS
PSS-mixkitm
5 x 500 mg
Mw Part [Da]*
PDI
Poly(dimethyl siloxane)
4 500
< 1.25
Poly(ethylene glycol)
6 000
< 1.25
Poly(methyl methacrylate)
5 000
< 1.15
Poly(styrene)
5 000
< 1.15
Poly(styrene sulfonate) sodium salt
5 000
< 1.20
*) F or all parts with a PDI ≠ 1 the molar mass produced will be within ±10% of the listed nominal molar mass (15-20% for broad standards). e.g. part
number PSS-ps700 will have an actual molar mass that is somewhere within the range of 630 - 770 Da. Exact values appear in the label of the vial
and Quality Certificate.
21
5|Standards for Membrane Characterization
The GPC/SEC membrane characterization is accomplished
by filtering the sample through the membrane.
Depending on the membrane type and quality some
smaller molecules can pass the pores of the membrane
while others will be retained. The filtered and the
un­filtered stock solution are then measured on a GPC/
SEC system. The average pore size distribution and the
cut-off the membrane is determined automatically by
comparing the elution profiles of the unfiltered sample
to the filtered fraction.
Following membrane standards are available:
•Linear Polysaccharide (Pullulan):
Part Nummber: PSS-mepulb300k (Mw: 300 000 Da)
•Branched Polysaccharide (Dextran):
P/N PSS-medxtb70k (Mw: 70 000 Da)
P/N PSS-medxtb2m (Mw: 2 000 000 Da)
•Polystyrene:
P/N PSS-mepsb200k (Mw: 200 000 Da)
Packing sizes: 50 g,100 g, 250 g, 500 g.
These new PSS membrane standards are featured by:
•
a broad molar mass distribution
•
the molar mass average Mw and Mn
•
the integral molar mass information Mmin, Mmax
•the corresponding radii of gyration Rgmin
and Rgmax.
Membrane Standards
GPC/SEC is a fast, robust and significant method for the
characterization of membranes, especially in their native
environment (swelled state). PSS offers the ability to
perform an automated, fast and simple membrane
characterization including cut-off and pore size distribution determination.
Reference Materials & LC Columns
6|GPC/SEC System Suitability Test with
PSS EasyValid Validation Kit
PSS has developed a dedicated GPC/SEC system
suitability test that evaluates the integral system:
equipment, electronics, and analytical operations, using
the PSS EasyValid Validation Kit. This ensures that the
system will yield validated GPC/SEC results.
The Validation Kit EasyValid is ideal:
The PSS EasyValid Validation Kit is designed for the
validation of GPC/SEC instrumentation with concentration
detectors independently of brand.
It consists of:
•
a validation column
•
calibration standards
•
certified reference materials
•
WinGPC Unity report layouts
•
WinGPC Unity import files
•
a comprehensive user documentation.
•
for checking the system performance
after installation
•
as part of the OQ/PV (Operational
Qualification/Performance Verification)
•
for performance review after maintenance
•
for inter laboratory consistence checks
•
for identifying systematic errors
•
for training new employees
For additional validation of molar mass sensitive
­detectors use Light Scattering / Viscometry Valdiation
Kits (see page 20)
EasyValid for organic systems
P/N PSS-pskitval
EasyValid for aqueous systems
P/N PSS-dxtkitval
Validation Report
22