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Instructions for Use
Oligonucleotide Calibration Standard (#206200)
Oligonucleotide mixture for calibration of matrix-assisted laser desorption
and ionization time-of-flight mass spectrometers (MALDI-TOF MS)
Product description
The oligonucleotide mixture allows calibrations and testing of MALDI-TOF mass spectrometers.
The mixture contains three standard oligonucleotides. The compounds are listed in the following
table together with their molecular weights. The standard oligonucleotides are supplied in five
tubes per package. Each tube containing 25 pmoles Oligo 12, 125 pmoles Oligo 20 and 500
pmoles Oligo 30. The quantity of substance allows 5x100 calibration points.
Oligonucleotide
Oligo 12 (12mer)
ACG TAC GTA CGT
Oligo 20 (20mer)
ACG TAC GTA CGT ACG TAC GT
Oligo 30 (30mer)
ACG TAC GTA CGT ACG TAC GTA CGT ACG TAC
[M+H]+
Average
[M]
Average
3646.4
3645.4
6118.0
6117.0
9192.0
9191.0
Storage and stability
The oligonucleotide tube box is shipped at ambient temperatures. We recommend storing the
oligonucleotides on arrival at less than 0 °C. Dissolved samples should be aliquoted and frozen.
We do not recommend refreezing samples after thawing.
Risk and safety information
This kit is not subject to classification according to the calculation methods of the “General
Classification Guideline for Preparations of the EU” as issued in the latest valid version. The
product is not subject to identification regulations under EU Directives and the Ordinance on
Hazardous Materials (GefStoffV). When used and handled according to specifications, the product
does not have any harmful effects according to our experience. All materials may present unknown
hazards and should be used with caution. Observe the general safety regulations when handling
chemicals.
Sample preparation procedure (recommendation)
Preliminary remarks
Poor sample preparation will degrade sensitivity, yield low resolution and poor reproducibility. The
generation of ions through MALDI depends on the production of a suitable composite material,
consisting of the matrix substance and the analyte. For best results use only chemicals of highest
available purity.
Chemicals and materials required
3-HPA
ACN
AHC
3-Hydroxypicolinic acid (1 g: #201224; 5 g: #203070)
Acetonitrile
Diammonium hydrogen citrate (100 mg/ml) in ultra pure water
Equipment and tools required: centrifuge, shaker, ultra sonic device, pipettes, pipette tips
1. Preparation of standard solution
Dissolve a sample of oligonucleotide calibration standard (contents of a tube) in 100 µl ultrapure
water and shake / whirl mix for several seconds (COligo 1 = 0.25 pmol/µl, COligo 2 = 1.25 pmol/µl,
COligo 3 = 5 pmol/µl).
2. Preparation of matrix solution
a) for standard steel targets:
- produce a solution of 3-HPA in ultrapure water / ACN (1:1 v/v) with a concentration of 50
mg/ml (if necessary, assist solution process by help of ultrasonic device)
- mix solution of 3-HPA with AHC (10:1 v/v)
b) for AnchorChipTM targets:
- mix 10 mg 3-HPA and 10 µl of AHC solution in 990 µl ultrapure water (if necessary, assist
solution process by help of ultrasonic device)
3. Preparation onto MALDI TOF-Target
-
Apply 1 µl matrix solution onto the target and allow drying at room temperature.
Add 1 µl standard solution and allow drying at room temperature once more.
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Results of measurement of oligonucleotide calibration standard
The oligonucleotide mixture is tested on a Bruker Daltonics autoflex® MALDI-TOF mass
spectrometer. Fig. 1 shows a typical spectrum obtained from the standard.
3646.4
6118.0
9192.0
4000
6000
8000
10000
Fig. 1: MALDI mass spectrum of oligonucleotide mixture.
Target preparation with 3-HPA matrix.
Ordering Information
Product
Oligonucleotide Calibration Standard LMW
Oligonucleotide Calibration Standard
Primer Extension Calibration Standard
Part No.
217028
206200
205930
Support
Email: [email protected]
Phone: +49 (421) 2205-0
Web: www.care-bdal.de
For research use only. Not for use in diagnostic procedures.
Revision 1, January 2012
Copyright 2012 Bruker Daltonik GmbH
Descriptions and specifications supersede all previous information and are subject to change without notice.
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