FES
validated with HTRF® KinEASE
TECHNICAL NOTE
Enzyme name : FES
Substrate : TK
Buffer additives : 5mM MgCl2, 1mM DTT, SEB
[FES] : 0,14 ng/µL
Incubation time at RT : 50 min
ATP Km : 17,9 µM
Staurosporine IC50 : 310 nM
Enzyme Titration & Time Course
1.000
0.333
0.111
0.074
0.025
0.008
0.003
0.000
15000
10000
5000
0
10
20
30
Time (min)
40
50
10000
Signal ratio (665/620)
Signal ratio (665/620)
20000
0
Staurosporine IC50
Enzyme (ng/ µl)
60
8000
6000
4000
2000
0
-12
-10
-8
-6
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FOR MORE INFORMATION
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Log Staurosporine (M)
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