Nephrology Dialysis Transplantation 31 (Supplement 1): i30–i31, 2016
doi:10.1093/ndt/gfw130.5
HYPERTENSION EXPERIMENTAL
MO010
MATERNAL BLOOD PRESSURE, RENAL FUNCTION AND
PLACENTAL DEVELOPMENT ARE AFFECTED IN
NORMOTENSIVE FEMALE RATS PREGNANT FROM
GENETICALLY HYPERTENSIVE MALES
pregnant from hypertensive males showed signs of placental insufficiency and FGR on
GD18 and 20 (Fig. 1C-D). At mid pregnancy (GD14), morphological alterations were
evident in the placenta, including an expansion of the labyrinth layer and thickened
walls of the uterine spiral arteries.
Conclusions: In conclusion, hypertension as a paternal factor influences the kinetics of
maternal blood pressure during pregnancy and compromises fetal growth, as a result of
placental dysfunction. Identification of placental factors contributed by the paternal
genotype may improve the understanding of disease mechanisms in hypertensive
disorders of pregnancy.
Gabriela Barrientos1, Ana Uceda1, Alejandra Sgariglia1, Graciela Giardina1,
Sandra M. Blois2 and Jorge E. Toblli1
1
Hospital Aleman School of Medicine, University of Buenos Aires, Laboratory of
Experimental Medicine, Ciudad Autonoma de Buenos Aires, ARGENTINA,
2
Charité University of Medicine, Department of Internal Medicine, Berlin,
GERMANY
Introduction and Aims: Severe forms of hypertensive disorders of pregnancy, like
early-onset preeclampsia (PE), are often linked with abnormal placentation. Genetic
factors contributed by the father may be important for placental development, and
previous studies support the existence of a paternal component for PE and fetal growth
restriction. Thus, the present study was conducted to evaluate the influence of arterial
hypertension as a paternal factor on maternal physiology, placental development and
fetal growth in normal female rats.
Methods: To this aim, female Wistar Kyoto rats (10-12 weeks old, N= 5animals/day
analysed) were mated to congenic (WKYxWKY, control pregnancy) or Stroke-Prone
Spontaneously Hypertensive Rat males (WKYxSHRSP, paternal effect model) and
checked daily for vaginal plugs, denoted as gestation day (GD) 1. Maternal blood
pressure (BP) was determined by the tail-cuff method pre-mating and on the morning
of GD1, 7, 9, 13, 17 and 19. On the selected dates, females were housed in metabolic
cages for collection of 24 h urine samples. Following collection of blood, animals were
euthanized and implantation sites isolated for morphological analyses. Fetal and
placental weights were recorded on GD 18 and GD 20
Results: As shown in Fig. 1A, reproductive performance and maternal adaptations to
pregnancy were impaired in females mated to hypertensive males. While BP in control
dams showed slight variations, SBP rose suddenly in females pregnant from SHRSP
males, peaking on GD9 and returning to pre-pregnancy levels at term (GD19, Fig 1B).
WKYxSHRSP dams displayed a significant up-regulation of basal albumin excretion
on GD14 (Fig 1C) respect to controls, consistent with defective renal function. Females
© The Author 2016. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved.