Illumina HiSeq4000/2500 and MiSEQ Sequencing Sample Submission Form
University of Colorado Anschutz Medical Campus
1. Contact Information
PI Name
Post Doc/Student/Tech
Institution
Address
City
PI Email
Email
Phone
State
Zip Code
2. Experiment and Sample Information
2.1 If you are submitting DNA/RNA samples for sequencing library preparation, please indicate sequencing type.
The optimal quantity of starting material is listed below. If sufficient RNA/DNA quantity and concentration is not
available, please consult the Core personnel before submitting.
Note: additional 100 to 200 ng of samples are required for sample QC/QA.
Genomic DNA Seq
100ng Genomic DNA in 50µl or less water/TE buffer
Small RNA Seq
1µg Total RNA (1µg to 5ug) in 5µl or less water/TE buffer
Exome Seq
1-3µg or 200ng Genomic DNA in 50µl water/TE buffer
ChIP Seq
1ng-10ng ChIP DNA in 10µl or less water/TE buffer
Directional mRNA Seq 1µg (500ng minimum) Total RNA in 50µl or less water/TE buffer
mRNA Seq
10 to 100ng in 10ul water/TE buffer (Ribo Removal/NoPolyA/Not Directional)
Directional Total RNA Seq Ribo Zero
1µg Total RNA (1µg to 4µg) in 10µl or less water/TE buffer
Other / Custom
Please attach / list below sample QA/QC data if any (Nano Drop, Pico Green, QPCR, Qubit, Bioanalyzer, etc)
2.2 If you are submitting ready-to-run sequencing libraries, please indicate the type of library.
Genomic DNA Seq
Small RNA Seq
mRNA Seq
Directional mRNA Seq
Directional Total RNA Seq Ribo Zero
ChIP Seq
Exome-Enrichment DNA Seq
Other
Please Indicate Length and Location of Tag:
Tag Sequence Length: (example 6 base pairs) _____________________
Dual Index: __________Yes
_______________ NO
Location of Tag: __________ Read 1 ___________ Index Read ____________Read 2
Other Tag Information we need to know: _________________________________________________________
___________________________________________________________________________________________
Are Custom Primers Required: ___________YES (Explain how to use them to us below) ___________ NO
___________________________________________________________________________________________
___________________________________________________________________________________________
___________________________________________________________________________________________
Please indicate what kit you used for sequencing library preparation:
________________________________________________________________________________________
Please attach library QA/QC data if any (Nano Drop, Pico Green, QPCR, Qubit, Bioanalyzer, etc).
PLEASE NOTE: It is customer’s responsibility to ensure your library is Illumina sequencing compatible. We will
do our best to get you the highest number of reads per lane, however there is no read number guarantee for
customer made sequencing libraries.
2.3 Sample information
1
2
Species of samples submitting
Sample names and concentration (if available) in the box below.(Add more rows if needed)
Sample Name
Concentration
Tag Sequence (if any)
2.4 Choose a sequencing run option below.
Addition of PhiX
____ YES
_____NO
Percent PhiX Spiked __________
HiSEQ 2500 HT Mode V4 Chemistry
_____ Single Read 50 cycles (1x50)
_____ Single Read 125 cycles (1x125)
_____ Paired End Read 125 cycles (2x125)
HiSEQ 4000 HT Mode
______ Single Read 50 (1x50)
_____ Single Read 150 cycles (1x150)
_____ Paired End Read 150- cycles (2x150)
HiSEQ 2500 HT Mode V3 Chemistry (only used when need CIF files)
_____ Single Read 50 cycles (1x50)
_____ Single Read 100 cycles (1x100)
_____ Paired End Read 100 cycles (2x100)
MiSEQ
_____ Single Read 50 cycles (1x50) V2 Chemistry
_____ Single Read 150 cycles (1x150) V3 Chemistry
_____ Paired End Read 150 cycles (2x150) V2 Chemistry
_____ Paired End Read 250 cycles (2x250) V2 Chemistry
_____Paired End Read 300 Cycles (2x300) V3 Chemistry
CUSTOM RUN: OTHER RUN OPTION State Machine HiSEQ 2000, HiSEQ 2500 or MiSEQ and Run Length Desired
________________________________________________________________________
2.5 How many flow cell lanes do you plan on using?
If multiplexing samples in a lane, please group your samples.
Lane
Samples
Lane 1
Lane 2
Lane 3
Lane 4
Lane 5
Lane 6
Lane 7
Lane 8
2.6 Data analysis/bioinformatics options
On our own (Email of person who will receive data) ______________________________________
By a designated bioinformatician
Bioinformatician Name:
By UCD Bioinformatics Core
Email:
3. Payment Information
3.1 University of Colorado Faculty
Speed Type
CCTSI or Cancer Center Member
___Yes ___No
3.2 Not Affiliated with the University of Colorado
An additional fee of 7.2% of total bill will be added to your invoice to cover University Associated Billing Fees
_______ Payment by Check
_______ Payment by Credit Card
_______ Payment by Wire Transfer
4. Sample Drop off or by Shipping
Email or call to schedule a time for sample drop off.
Contact
Contact
Hours of operation
for sample drop off
Location/ Shipping
Address
Katrina Diener
[email protected]; [email protected]
(303) 724- 6050
Todd
[email protected]
Woessner
(303) 724-6047
9:00am to 3:00pm
Monday – Friday, excluding university holidays.
Genomics and Microarray Core
University of Colorado Anschutz Medical Campus
12700 E. 19th Ave
Bldg: RC-2, Room 9400
Aurora, CO 80045
5. Required Signatures
Please have both the Principle Investigator (PI) that will be paying for the Sequencing Services and the
Research/Technician/Student/ Post-Doc preparing the samples sign below acknowledging that all of the information
provided on the form is correct. Signature of this form acknowledges that the PI and Technician/Student/Post-Doc have
agreed to all sample submission, quality, quantity, project scheduling, and researcher financial responsibility
requirements. Signature of this form authorizes the UC Genomics and Microarray Core to order all consumables
necessary for the researcher’s sequencing project and confirms that the PI is financially responsible for items ordered for
their project and all labor cost associated with the project.
Principle Investigator Signature
Date
Technician/Student/Post-Doc Signature
Date
6. Data and Sample Retrieval
All data from sequencing run will be deleted from our servers 31 days after you receive notification that your data is
ready to be downloaded from our server via email.
Please pick up original DNA / RNA submitted along with constructed libraries no later than 60 days after data has been
downloaded from our server. If you do not pick up your samples and libraries they will be destroyed.