HICHROM
Chromatography Columns and Supplies
LC COLUMNS
Shodex
Catalogue 9
Hichrom Limited
1 The Markham Centre, Station Road
Theale, Reading, Berks, RG7 4PE, UK
Tel: +44 (0)118 930 3660 Fax: +44 (0)118 932 3484
Email: [email protected] www.hichrom.co.uk
Please contact Hichrom for current prices. Tel: +44 (0)118 930 3660 Email: [email protected]
Shodex
LC Columns - Shodex
SHODEX®
• Silica- and polymer-based phases
• Columns for all chromatographic modes
• Wide application range
• Capillary, microbore, analytical and preparative dimensions
Showa Denko K.K., Japan manufactures the Shodex® line of HPLC columns for the pharmaceutical and biotechnology industries. A wide range of products
with both silica-based and polymer-based materials is available. An overview of the products is shown on the following pages: reversed-phase (page 210),
ligand exchange (page 211), ion-exchange and ion chromatography (page 212), size exclusion (page 213), HIC (page 214) and affinity (page 214). Please
enquire for additional details.
Shodex Reversed-Phase Columns
The Shodex product range contains both polymer-based and silica-based phases. Please enquire for ordering details where not listed.
Reversed-Phase (Polymer-based) Phases
The table below lists the polymer-based phases available.
Column Series
Base Material
Functional Group
Comments/Applications
Styrene-divinylbenzene
-
Analysis of proteins and peptides
DE, GOLF, CARB
Polymethacrylate
-
DE series columns have similar selectivity to C18 columns
DM-614
Polyhydroxymethacrylate
-
Suitable for analysis of amino acids and polypeptides
RSpak
NN
Polyhydroxymethacrylate
Sulpho
RSpak
JJ-50
Polyvinyl alcohol
Quaternary ammonium
Suitable for separation of mixtures of neutral and ionic
compounds
RSpak
RP18, DS
RSpak
RSpak
Asahipak
ODP
Polyvinyl alcohol
Octadecyl
Asahipak
ODP2 HP
Polyhydroxymethacrylate
Octadecyl
Octyl
Asahipak
C8P-50
Polyvinyl alcohol
Asahipak
C4P-50
Polyvinyl alcohol
Butyl
Asahipak
NH2P
Polyvinyl alcohol
Amino
Large pore size phases, suitable for high molecular weight
compounds eg. proteins, as well as low molecular weight
compounds. LC-MS applications
Analysis of saccharides (NP)
Shodex ODP2 HP is a macroporous polyhydroxymethacrylate reversed-phase material offering better efficiencies than many other resin-based phases
and comparable to that of silica-based C18 columns. It has a recommended pH range of 3 to 12. The high polarity and small pore size (40Å) of this phase
is designed to exclude proteins and can be used for the analysis of drugs in biological samples ie. as restricted access material (see Figure 1 for example).
Blank
switch valve
introduce to MS
UV, 210nm
with BSA
BSA
switch valve
introduce to MS
Barbital
0.0
Barbital
0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 Mins
0.0
SIM : m/z=183
Barbital
Peak Area : 100%
For free technical advice and support email [email protected]
210
0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 Mins
Barbital
Peak Area : 99%
0.0
Sample: 5μl
Barbital: 500μg/l
BSA: 7.0mg/ml
0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 Mins
SIM : m/z=183
0.0
UV, 210nm
Column: Shodex ODP2 HP-2B (50 x 2.0mm)
Eluent:10mM CH3COONH4 – CH3CN (70:30)
Flow rate: 0.2ml/min
Column temperature: 30°C
Detector: UV, 210nm and ESI-MS (-ve SIM)
0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 Mins
Figure 1. LC-MS of barbital in BSA
Ordering Information - Shodex ODP2 HP
Product
1
Particle Size (µm)
Column Dimensions (mm)
Catalogue No.
Price
ODP2 HP-4B
5
50 x 4.6
F7622001
£306
ODP2 HP-4D
5
150 x 4.6
F7622002
£340
ODP2 HP-4E
5
250 x 4.6
F7622003
£352
ODP2 HPG-4A
5
10 x 4.6 (guard column, 1/pk)
F6714010
£212
ODP2 HP-4GC
5
10 x 4.0 (guard cartridge, 3/pk)1
F6714012
£287
ODP2 HP-2B
5
50 x 2.0
F7622004
£306
ODP2 HP-2D
5
150 x 2.0
F7622005
£340
ODP2 HPG-2A
5
10 x 2.0 (guard column, 1/pk)
F6714011
£212
Use with guard cartridge holder HLD-4GC (Cat. No. F8700020, £210).
Reversed-Phase (Silica-based) Phases
Please enquire for details of reversed-phase silica-based columns, including Silica NPE (nitrophenylethyl) and Silica PYE (pyrenylethyl).
Please contact Hichrom for current prices. Tel: +44 (0)118 930 3660 Email: [email protected]
Shodex
Ligand Exchange Phases (Shodex SUGAR Series)
Column Series
SUGAR
SH
SUGAR
SC
SUGAR
SP
SUGAR
SZ
SUGAR
KS
USPpak
MN
Base Material
Styrene-divinylbenzene
Styrene-divinylbenzene
Styrene-divinylbenzene
Styrene-divinylbenzene
Styrene-divinylbenzene
Styrene-divinylbenzene
Functional Group
Sulpho (H+)
Sulpho (Ca2+)
Sulpho (Pb2+)
Sulpho (Zn2+)
Sulpho (Na+)
Sulpho (Ca2+)
Comments/Applications
Separation of saccharides by ligand exchange,
size exclusion, ion exclusion and normal-phase.
Suitable for separation of sugars and sugar alcohols
Separation of saccharides by size exclusion and ligand exchange
Analysis of mannitol by USP method
There are two series of Shodex® columns for saccharide analysis –
the SUGAR series for operation in ligand exchange mode and
Asahipak NH2P-50 polymeric amino columns for normal-phase and
HILIC modes.
1. Fructose
2. Glucose
3. Sucrose
4. Maltose
1
LC Columns - Shodex
Shodex® Columns for Saccharide Analysis
180 h
1
3
3
2
2
A. Shodex SUGAR Series
The SUGAR series columns are based on polystyrene-divinylbenzene
copolymer with strong cation-exchange resin incorporating functional
sulpho groups coupled with metal counter ions (H+, Ca2+, Pb2+, Zn2+, Na+).
4
4
B. Shodex Asahipak NH2P
Shodex Asahipak NH2P is an amino bonded column in which polyamine is
bonded to a hydrophilic polyvinyl alcohol polymer. The high separation
power of conventional silica-based columns is maintained, whilst
showing an increased usable pH range (2 – 13) and increased robustness.
Figure 2 illustrates the reproducibility over time obtained from the polymeric
Asahipak NH2P column.
0
10
20 Mins
0
10
20 Mins
Column: Shodex Asahipak NH2P-50 4E (250 x 4.6mm)
Eluent: CH3CN – H2O (75:25)
Flow rate: 1.0ml/min
Column temperature: 30°C
Detection: RI
Figure 2. Reproducibility with Asahipak NH2P-50
Ordering Information - Columns for Saccharide Analysis
Product
1
Separation
Mode
SEC + IEX
SEC + IEX
SEC + LEX
SEC + LEX
SEC + LEX
SEC + LEX
NP + LEX
NP + LEX
SEC + LEX
SEC + LEX
SEC
SEC
SEC
SEC
SEC
LEX
Exclusion Limit
(Da)
1,000
10,000
Guard column
1,000
1,000
10,000
Guard column
1,000
Guard column
Guard column
Guard column
1,000
10,000
50,000
400,000
5,000,000
50,000,000
Guard column
200,000,000
Guard column
-
Particle Size
(µm)
6
6
10
6
6
6
10
7
10
6
10
6
6
6
6
6
7
17
17
10
30
30
8
Column Dimensions1
(mm)
300 x 8.0
300 x 8.0
50 x 6.0
300 x 8.0
300 x 7.8
300 x 8.0
50 x 6.0
300 x 8.0
50 x 6.0
250 x 6.0
10 x 4.6
150 x 6.0
10 x 4.6
300 x 8.0
300 x 8.0
300 x 8.0
300 x 8.0
300 x 8.0
300 x 8.0
50 x 6.0
300 x 8.0
50 x 8.0
250 x 4.0
-
NP/HILIC
NP/HILIC
NP/HILIC
NP/HILIC
-
Guard column
Guard column
Guard column
Line filter
4
5
5
5
5
5
5
-
250 x 3.0
10 x 3.0
150 x 4.6
250 x 4.6
10 x 4.6
150 x 2.0
10 x 2.0
75 x 8.0
Catalogue No.
Price
F6378100
F6378101
F6700080
F6378102
F6379300
F6378103
F6700090
F6378105
F6700081
F7001400
F6700120
F7001300
F6700110
F6378010
F6378020
F6378025
F6378035
F6378050
F6378060
F6700020
F6378070
F6700021
F6379230
£859
£859
£307
£859
£696
£859
£307
£859
£307
£859
£307
£859
£307
£904
£904
£904
£904
£904
£904
£307
£1,226
£331
£853
F7630007
F6710030
F7630002
F7630001
F6710016
F7630006
F6713000
F6710100
£724
£318
£649
£704
£276
£683
£276
£338
Preparative columns also available
www.hichrom.co.uk • [email protected] • Tel: 0118 930 3660
Hichrom Limited
SUGAR SH1011
SUGAR SH1821
SUGAR SH-G
SUGAR SC1011
SUGAR EP SC1011-7F
SUGAR SC1821
SUGAR SC-LG
SUGAR SP0810
SUGAR SP-G
SUGAR SC1211
SUGAR SC-G
SUGAR SZ5532
SUGAR SZ-G
SUGAR KS-801
SUGAR KS-802
SUGAR KS-803
SUGAR KS-804
SUGAR KS-805
SUGAR KS-806
SUGAR KS-G
SUGAR KS-807
SUGAR KS-807G
USPpak MN-431
Asahipak Phases
NH2P-40 3E
NH2P-50G 3A
NH2P-50 4D
NH2P-50 4E
NH2P-50G 4A
NH2P-50 2D
NH2P-50G 2A
NH2P-LF
Counter
Ion
+
H
+
H
+
H
Ca2+
Ca2+
Ca2+
Ca2+
Pb2+
Pb2+
Ca2+
Ca2+
Zn2+
Zn2+
+
Na
+
Na
+
Na
+
Na
+
Na
+
Na
+
Na
+
Na
+
Na
2+
Ca
211
Please contact Hichrom for current prices. Tel: +44 (0)118 930 3660 Email: [email protected]
LC Columns - Shodex
Shodex
Shodex® Ion-Exchange Phases
Ion-Exchange Phases
Base Material
Polyhydroxymethacrylate
Functional Group
Quaternary ammonium
DEAE
Polyhydroxymethacrylate
Diethylaminoethyl
IEC
SP
Polyhydroxymethacrylate
Sulphopropyl
IEC
CM
Polyhydroxymethacrylate
Carboxymethyl
IEC
Column Series
QA
IEC
Comments/Applications
Strong anion-exchange
Suitable for analysis of
Weak anion-exchange
proteins, peptides, DNA,
Strong cation-exchange
RNA, oligonucleotides
Weak cation-exchange
Asahipak
ES-502N
Polyvinyl alcohol
Diethylaminoethyl
Weak anion-exchange
Asahipak
ES-502C
Polyvinyl alcohol
Carboxymethyl
Weak cation-exchange
AXpak
WA
Polyhydroxymethacrylate
Diethylaminoethyl
CXpak
P
Styrene-divinylbenzene
Sulpho (Na )
PIKESS
DEAE
Polyhydroxymethacrylate
Diethylaminoethyl
Weak anion-exchange
PIKESS
SP
Polyhydroxymethacrylate
Sulphopropyl
Strong cation-exchange
Weak anion-exchange, suitable for analysis of nucleic acids
Analysis of amino acids and amino sugars
+
UHPLC compatible
(2.5µm material)
The IEC series (QA and DEAE anion-exchange phases and SP and CM cation-exchange phases) is based on polyhydroxymethacrylate. Columns are
suitable for the analysis of relatively high molecular weight compounds, such as proteins. The newer PIKESS materials are non-porous 2.5µm particles,
suitable for rapid protein analyses using UHPLC systems (see Figure 3).
1
2
5
3
2
6
4
0.0
Column: Shodex PIKESS SP-2B (50 x 2.0mm)
Eluent: A: 20mM Na phosphate, pH 7.0
B: A + 0.5M NaCl
Linear gradient, 100% A to 50% A in 2.5min
Flow rate: 1.2ml/min
Detection: UV, 280nm
1.0
2.0
3.0
4.0 Mins
Column: Shodex IC YS-50 (125 x 4.6mm)
Eluent: 4mM methanesulphonic acid
Flow rate: 1.0ml/min
Column temperature: 40°C
Detector: Non-suppressed conductivity
5
0.0
Figure 3. Rapid analysis of proteins using UHPLC and ion-exchange
4
1
5µ S/cm
1. Myoglobin
2. Trypsinogen
3. Ribonuclease A
4. a-Chymotrypsinogen A
5. Cytochrome C
6. Lysozyme
3
5
10
6
15
1. Li+​ 2ppm
2. Na+​ 10ppm
3. NH4+​ 10ppm
4. K+​ 20ppm
5. Mg2+​ 10ppm
6. Ca2+​ 20ppm
20 Mins
Figure 4. Separation of cations using ion chromatography
Please enquire for ordering details of ion-exchange columns.
Shodex Ion Chromatography Phases
Ion Chromatography Phases
For free technical advice and support email [email protected]
Column Series
IC
NI, I
IC
SI
212
Base Material
Polyhydroxymethacrylate
Polyvinyl alcohol
Functional Group
Quaternary ammonium
Quaternary ammonium
IC
Y, T, R
Styrene-divinylbenzene
Sulpho
IC
IC
YK
YS
Silica
Silica
Carboxyl
Carboxyl
Comments/Applications
Anion analysis with non-suppressed detection
Anion analysis with suppressed detection
Cations by non-suppressed detection. Y for alkylamines.
T for transition metals and R for rare earth metals by
post-column reaction
Monovalent and divalent cations by non-suppressed detection
Higher performance version of YK
A range of Shodex columns are available for both non-suppressed and suppressed ion chromatography. Columns are also supplied for analysing transition
metal ions and rare earth metal ions using post-column reactions. Figure 4 shows the separation of six common cations on a Shodex IC YS-50 column
with non-suppressed conductivity detection.
Ordering Information - Columns for Ion Chromatography
1
Column
Particle Size (µm)
Column Dimensions (mm)
Catalogue No.
Price
Guard Column
Catalogue No.
Price
IC NI-424
5
100 x 4.6
F6995243
£845
IC NI-G
F6709616
£283
IC I-524A
12
100 x 4.6
F6995240
£767
IC IA-G
F6700400
£283
IC Y-521
12
150 x 4.6
F6995210
£806
IC Y-G
F6700230
£283
IC YK-421
5
125 x 4.6
F7120012
£708
IC YK-G
F6709608
£252
IC YS-50
5
125 x 4.6
F7122000
£1,061
IC YS-G
F6700530
£276
1
IC SI-90 4E
9
250 x 4.0
F6995244
£782
1
IC SI-90G
F6709620
£283
IC SI-50 4E1
5
250 x 4.0
F6995245
£971
IC SI-50G1
F6709625
£283
IC T-5211
12
150 x 4.6
F6995250
£979
IC T-G1
F6700412
£331
IC R-621
5
50 x 6.0
F6998000
£806
IC R-G
F6709090
£283
PEEK hardware
Please contact Hichrom for current prices. Tel: +44 (0)118 930 3660 Email: [email protected]
Shodex
Aqueous SEC (GFC) Phases
Column Series
Base Material
Functional Group
PROTEIN
KW
Silica
Hydrophilic polymer
OHpak
SB
Polyhydroxymethacrylate
-
Comments/Applications
Analysis of high MW biological fluids. Suitable for separating
proteins of MW few thousand to few million Da
Analysis of water-soluble samples and for molecular weight
distribution
PROTEIN KW-800 and KW400 are silica based series with high protein recovery rates. Whereas the KW-800 columns have the highest sample loading,
the reduced particle size of KW400 columns enable 3-4 times greater sensitivity. Figure 5 illustrates the enhanced sensitivity achieved for a range of
proteins with a KW400 column.
5
1. Blue dextran 2000​ 0.2mg/ml
2. g-Globulin​
0.8mg/ml
3. Ovalbumin​
0.8mg/ml
4. Myoglobin​
0.56mg/ml
5. Uridine​0.04mg/ml
3
1
Columns: (A) Shodex KW402.5-4F (300 x 4.6mm)
(B) Shodex PROTEIN KW-802.5 (300 x 8mm)
Eluent: 50mM Na phosphate buffer, pH 7.0 + 0.3M NaCl
Flow rate: (A) 0.33ml/min (B) 1.0ml/min
Detector: UV, 280nm
Sample volume: 10µl
5
4
2
(A) KW402.5-4F
0
LC Columns - Shodex
Shodex® GFC Phases
1
(B) KW-802.5
5
10
Mins
15
0
5
2
3
4
10
Mins
15
Figure 5. Comparison between KW400 and KW-800 columns
The OHpak SB-800 and SB400 series are based on polyhydroxymethacrylate and are used for the analysis of a wide range of molecular weight compounds.
Ordering Information – GFC Columns
Pore Size (Å)
Exclusion Limit (Da)
PROTEIN
Product
Particle Size
(µm)
Average
Pullulan
Protein
Column Dimensions
(mm)
Catalogue
No.
Price
KW-802.5
5
150
KW-803
5
300
400
60,000
150,000
300 x 8.0
F6989000
£877
1,000
170,000
700,000
300 x 8.0
F6989103
KW-804
7
500
1,500
£877
500,000
1,000,000
300 x 8.0
F6989104
£877
150
400
60,000
50 x 6.0
F6700131
£307
150,000
300 x 4.6
F6989201
£983
150,000
F6989202
£983
Maximum
KW-G
7
KW402.5-4F
3
KW403-4F
3
250
800
600,000
300 x 4.6
KW404-4F
5
500
1,500
500,000
1,000,000
300 x 4.6
F6989203
£983
KW405-4F
5
700
2,000
1,300,000
20,000,000
300 x 4.6
F6989204
£983
KW400G-4A
5
10 x 4.6
F6700132
£236
Column Dimensions
(mm)
Catalogue
No.
Price
OHpak Product
Particle Size
(µm)
Guard column
Guard column
Pore Size (Å)
(Maximum)
Exclusion Limit (Da)
Pullulan
SB-802 HQ
8
100
4,000
300 x 8.0
F6429100
£960
SB-802.5 HQ
6
200
10,000
300 x 8.0
F6429101
£960
SB-803 HQ
6
800
100,000
300 x 8.0
F6429102
£960
SB-804 HQ
10
2,000
1,000,000
300 x 8.0
F6429103
£960
SB-805 HQ
13
7,000
4,000,000
300 x 8.0
F6429104
£960
SB-806 HQ
13
15,000
20,000,000
300 x 8.0
F6429105
£960
SB-806M HQ
13
15,000
20,000,000
300 x 8.0
F6429106
£960
50 x 6.0
F6709430
£307
500,000,000
300 x 8.0
F6429108
£1,179
50 x 8.0
F6709431
£307
1,000
250 x 4.6
F6429111
£960
SB-G
10
SB-807 HQ
35
SB-807G
35
SB401-4E
10
Guard column
30,000
Guard column
40
6
200
10,000
250 x 4.6
F6429112
£960
6
800
100,000
250 x 4.6
F6429113
£960
SB404-4E
7
2,000
1,000,000
250 x 4.6
F6429114
£960
SB400G-4A
7
10 x 4.6
F6709432
£307
Guard column
www.hichrom.co.uk • [email protected] • Tel: 0118 930 3660
Hichrom Limited
SB402.5-4E
SB403-4E
213
LC Columns - Shodex
Shodex
Other Shodex® Phases
Aqueous/Organic SEC Phases
Column Series
Asahipak
GF
Base Material
Functional Group
Polyvinyl alcohol
None
Comments/Applications
Both water and organic solvents can be used. Suitable for
samples with both hydrophilic and hydrophobic moieties.
The Asahipak GF Series columns are based on polyvinyl alcohol and can be used with both aqueous and organic solvents.
Please enquire for further details and ordering information for aqueous/organic SEC phases.
Organic SEC (GPC) Phases
Column Series
KF, K, KD,
HFIP, LF, HT,
UT, AT
GPC
Base Material
Functional Group
Styrene-divinylbenzene
None
Comments/Applications
KF-800 series shipped in THF. K-800 series shipped in CHCl3.
KD-800 series shipped in DMF – suitable for assay of polar
polymers. HFIP suitable for engineering plastics eg. PET,
polyamides. HT, UT and AT series for higher temperature GPC.
Please enquire for further details and ordering information for organic SEC (GPC) phases.
Hydrophobic Interaction Phase
HIC
Column Series
PH
Base Material
Polyhydroxymethacrylate
Functional Group
Phenyl
Comments/Applications
Analysis of proteins
The HIC PH-814 column separates proteins without denaturation. It is applicable to samples after treatment of ammonium sulphate fractions.
Organic Acids Phase
Column Series
RSpak
KC
Base Material
Styrene-divinylbenzene
Functional Group
Comments/Applications
Sulpho
Analysis of organic acids by ion exclusion and
reversed-phase modes
Shodex® RSpak KC columns are suitable for the analysis of organic acids by ion exclusion and reversed-phase modes.
Affinity Phases
Column Series
AFpak
6 kinds
Base Material
Functional Group
Polyhydroxymethacrylate
6 kinds of ligand
Comments/Applications
Purification of biological molecules depending on
biochemical affinity
The Shodex AFpak range of affinity columns consists of 6 different ligand materials, designed for specific applications.
For free technical advice and support email [email protected]
Chiral Separation Phases
214
Column Series
CDA, CDB,
ORpak
CDC
ORpak
CDBS
Base Material
Functional Group
Polyhydroxymethacrylate
Cyclodextrin derivative
Silica
Cyclodextrin derivative
Comments/Applications
Suitable for separation of optical isomers and structural
isomers. CDA is a-CD, CDB is b-CD , CDC is g-CD
CDBS is b-CD
ORpak
Polyhydroxymethacrylate
L-amino acid derivative
Separation of amino acids by ligand exchange
CRX
Shodex ORpak CD phases consist of a-, b- or g-cyclodextrin bonded to polyhydroxymethacrylate base material or silica (a-cyclodextrin). Shodex ORpak
CRX columns have an L-amino acid derivative bonded to polyhydroxymethacrylate and are suitable for the separation of optical isomers of amino acids
and hydroxy acids.
Please contact Hichrom for further information on any Shodex column and for ordering information for columns not listed.