5.0 PCR Tubes
General Information
Free of human DNA, certified free of
DNase, RNase and PCR inhibitors
AHN Biotechnologie PCR tubes
AHN PCR-Micro Test Tubes basically withstand the thermal and mechanical strain of
being heated in a thermoblock or being subjected to high-speed centrifugation. It is
possible to open and close them using one hand only, without any risk of the sample being
contaminated. If the PCR-Tube fail to fulfill these requirements, several errors may occur
which may threaten the success or the reproducibility of the experiment. It is therefore
essential to select the correct Micro Test Tube like AHN PCR-Tubes. They are availabe in
2 volumes, 0.2 ml and 0.5 ml, with optimized clear transparency. These tubes are
available in different colours and are manufactured in clean rooms under DNA-free,
RNase-free, ATP-free and pyrogene-free conditions. Optimized for all thermocyclers AHN
customer can choose between 2 designs with flat caps or domed caps. Autoclavable when
open (121° C, 20 min).
AHN Biotechnologie PCR tubes offer excellent quality
Top quality test tubes, like AHN PCR-Tubes, can only be manufactured by using high
precision injection molds and the best available raw materials, high quality polymers. AHN
Biotechnologie PCR-Tubes are continually developed and modified to meet exact
customer demands. They withstand extreme mechanical strain and demonstrate excellent
chemical resistance and standards of purity. The demands placed on plastic disposables
have increased. In fact, the success, and indeed the very existence of many methodes
currently used in the fields of molecular biology and biochemistry would not have been
possible without high quality plastic consumables such as AHN Biotechnologie PCR-tubes.
AHN Biotechnologie PCR tubes thin-walled design
Reduces cycle times and improves product specifity by proving accurate, rapid and
uniform heat transfer.
AHN Biotechnologie PCR tubes sealing, oil free
Providing 100 % sealing without the need for an oil overlay, when used in conjunction with
a thermal cycler fitted with a heated lid.
AHN Biotechnologie PCR tubes cap design
Flat cap designed products are ideal for those concerned with contamination and provides
handling convenience for low throughput usage. Domed cap products are designed to
facilitate efficient heat transfer with “hot-lid” thermal cyclers.
AHN Biotechnologie PCR tubes and qPCR-capillaries packaging configurations
AHN PCR tubes are packed loose in safety welded polyethylene bags. They can be used for nonsterile or sterile procedures or for refilling any rack system. For your budget, AHN bulk packs are
the most economical way to purchase the best laboratory plastic products available. Each bag is
labelled with a sticker. 10 bags are packed in a carton. qPCR capillaries are packed also in
rackboxes (8x12 matrix) or in bags with 100 capillaries.
AHN PCR tubes demonstrate excellent chemistry resistance and standards of purity
and quality !!!
AHN Biotechnologie Catalogue 2009/2010
Page 25
5.1 PCR Tubes
Thin walled volume 0.2 ml
Free of human DNA, certified free of
DNase, RNase and PCR inhibitors
AHN PCR tubes are available in different colours and
are manufactured in clean rooms under DNA-free,
RNase-free, ATP-free and pyrogene-free conditions.
Optimized for all thermocyclers AHN customer can
choose between 2 designs with flat caps or domed
caps. Autoclavable when open (121° C, 20 min).
AHN thin-walled PCR-Tubes basically
withstand the thermal and mechanical
strain of being heated in a
thermoblock or being subjected to
high-speed
centrifugation.
It
is
possible to open and close them using
one hand only, without any risk of the
sample being contaminated. If the
PCR-Tube fail to fulfill these
requirements, several errors may
occur which may threaten the success
or
the
reproducibility
of
the
experiment. It is therefore essential to
select PCR tubes like AHN PCR
tubes. They are availabe in 2 volumes,
0.2 ml and 0.5 ml with optimized clear
transparency.
The thin-walled design reduces
cycle times and improves product
specifity by proving accurate, rapid
and uniform heat transfer !
AHN PCR tubes demonstrate excellent chemistry resistance and standards of purity!
Cat.no.
3-010-50-0
3-011-50-0
3-010-50-1
3-010-50-2
3-010-50-8
3-010-80-0
3-010-80-1
3-010-80-2
3-010-80-8
Description *
AHN PCR tubes 0.2 ml flat cap
AHN PCR tubes 0.2 ml flat cap
AHN PCR tubes 0.2 ml flat cap
AHN PCR tubes 0.2 ml flat cap
AHN PCR tubes 0.2 ml flat cap
AHN PCR tubes 0.2 ml flat cap
AHN PCR tubes 0.2 ml flat cap
AHN PCR tubes 0.2 ml flat cap
AHN PCR tubes 0.2 ml flat cap
3-020-50-0
3-021-50-0
3-020-50-1
3-020-50-2
3-020-50-8
3-020-80-0
3-020-80-1
3-020-80-2
3-020-80-8
AHN PCR tubes 0.2 ml domed cap
AHN PCR tubes 0.2 ml domed cap
AHN PCR tubes 0.2 ml domed cap
AHN PCR tubes 0.2 ml domed cap
AHN PCR tubes 0.2 ml domed cap
AHN PCR tubes 0.2 ml domed cap
AHN PCR tubes 0.2 ml domed cap
AHN PCR tubes 0.2 ml domed cap
AHN PCR tubes 0.2 ml domed cap
Sterile
non
yes
non
non
non
non
non
non
non
Colour
natural
natural
yellow
blue
magenta
natural
Yellow
blue
magenta
Pack type
bag
bag
bag
bag
bag
bag
bag
bag
bag
non
yes
non
non
non
non
non
non
non
natural
natural
yellow
blue
magenta
natural
yellow
blue
magenta
bag
bag
bag
bag
bag
bag
bag
bag
bag
Pack size Sales unit
1000
1 x 1000
1000
1 x 1000
1000
1 x 1000
1000
1 x 1000
1000
1 x 1000
1000
10 x 1000
1000
10 x 1000
1000
10 x 1000
1000
10 x 1000
1000
1000
1000
1000
1000
1000
1000
1000
1000
1 x 1000
1 x 1000
1 x 1000
1 x 1000
1 x 1000
10 x 1000
10 x 1000
10 x 1000
10 x 1000
* Suitable racks for AHN PCR-tubes you will find on page 35 !
AHN Biotechnologie Catalogue 2009/2010
Page 26
5.2 PCR Tubes
Thin walled volume 0.5 ml
Free of human DNA, certified free of
DNase, RNase and PCR inhibitors
AHN PCR tubes are available in different colours and
are manufactured in clean rooms under DNA-free,
RNase-free, ATP-free and pyrogene-free conditions.
Optimized for all thermocyclers AHN customer can
choose between 2 designs with flat caps or domed
caps. Autoclavable when open (121° C, 20 min).
Top quality PCR tubes, like AHN PCR
tubes, can only be manufactured by
using high precision injection molds
and the best available raw materials,
high
quality
polymers.
AHN
Biotechnologie
PCR-Tubes
are
continually developed and modified to
meet exact customer demands. They
withstand extreme mechanical strain
and demonstrate excellent chemical
resistance and standards of purity.
Providing 100 % sealing without the
need for an oil overlay, when used in
conjunction with a thermal cycler fitted
with a heated lid.
Flat cap designed products are ideal
for
those
concerned
with
contamination and provides handling
convenience for low throughput usage.
Domed cap designed tubes offers a
domed cap to facilitate efficient heat
transfer with “hot-lid” thermal cyclers.
AHN PCR tubes demonstrate excellent chemistry resistance and
standards of purity !
Cat.no.
3-110-50-0
3-111-50-0
3-110-50-1
3-110-50-2
3-110-50-8
3-110-80-0
3-110-80-1
3-110-80-2
3-110-80-8
Description *
AHN PCR tubes 0.5 ml flat cap
AHN PCR tubes 0.5 ml flat cap
AHN PCR tubes 0.5 ml flat cap
AHN PCR tubes 0.5 ml flat cap
AHN PCR tubes 0.5 ml flat cap
AHN PCR tubes 0.5 ml flat cap
AHN PCR tubes 0.5 ml flat cap
AHN PCR tubes 0.5 ml flat cap
AHN PCR tubes 0.5 ml flat cap
3-120-50-0
3-121-50-0
3-120-50-1
3-120-50-2
3-120-50-8
3-120-80-0
3-120-80-1
3-120-80-2
3-120-80-8
AHN PCR tubes 0.5 ml domed cap
AHN PCR tubes 0.5 ml domed cap
AHN PCR tubes 0.5 ml domed cap
AHN PCR tubes 0.5 ml domed cap
AHN PCR tubes 0.5 ml domed cap
AHN PCR tubes 0.5 ml domed cap
AHN PCR tubes 0.5 ml domed cap
AHN PCR tubes 0.5 ml domed cap
AHN PCR tubes 0.5 ml domed cap
Sterile
non
yes
non
non
non
non
non
non
non
Colour
natural
natural
yellow
blue
magenta
natural
Yellow
blue
magenta
Pack type
bag
bag
bag
bag
bag
bag
bag
bag
bag
non
yes
non
non
non
non
non
non
non
natural
natural
yellow
blue
magenta
natural
yellow
blue
magenta
bag
bag
bag
bag
bag
bag
bag
bag
bag
Pack size Sales unit
1000
1 x 1000
1000
1 x 1000
1000
1 x 1000
1000
1 x 1000
1000
1 x 1000
1000
10 x 1000
1000
10 x 1000
1000
10 x 1000
1000
10 x 1000
1000
1000
1000
1000
1000
1000
1000
1000
1000
1 x 1000
1 x 1000
1 x 1000
1 x 1000
1 x 1000
10 x 1000
10 x 1000
10 x 1000
10 x 1000
* Suitable racks for AHN PCR-tubes you will find on page 35 !
AHN Biotechnologie Catalogue 2009/2010
Page 27
5.3 PCR-Stripes
Thin walled volume 0.2 ml
Free of human DNA, certified free of
DNase, RNase and PCR inhibitors
The efficiency and flexibility of AHN
Biotechnologie PCR tube-strips is
ensured by the fact that they all are
manufactured from Polypropylene.
The recovery of sample products is
increased
in
comparison
to
experiments
carried
out
using
polycarbonate equipment, and the risk
of biological contamination is reduced.
Safety and flexibility are also
increased as polypropylene equipment
can be autoclaved and sealed using
many different methods.
AHN PCR tube-stripes are manufactured in
clean rooms under DNA-free, RNase-free, ATPfree and pyrogene-free conditions. Optimized
for all thermocyclers they are autoclavable
when open (121° C, 20 min).
Specification
CE-marking certificate
Sterility certificate
RoHS Compliance certificate
Environmental Management certificate
USP-Plastic Test, Class VI, certificate
GMP/GLP certificate
Cat.no.
3-030-40-0
3-031-40-0
3-040-40-0
3-041-40-0
The stripes are available each with 8or -12 tubes. The separate domed
caps are also delivered as 8- or 12caps stripes and seal safety the tubes.
.
Domed cap designed tubes offers a
domed cap to facilitate efficient
heat transfer with “hot-lid”
thermocyclers.
According to
EU-Directive 98/79/EG, competent authority code DE/CA90
EN 552/ISO 11137 and DIN EN 556
EU Directive 2002/95/EC
ISO Standard 14000 and ISO Standard 14001
ISO Standard 10993
WHO Technical Report Series 908, 37. Report, Geneva 2003
Description *
Sterile
AHN PCR 8-tubes-stripes 0.2 ml non
and 8-cap stripes (domed caps)
AHN PCR 8-tubes-stripes 0.2 ml
yes
and 8-cap stripes (domed caps)
AHN PCR 12-tubes-stripes 0.2 ml non
and 12-cap stripes (domed caps)
AHN PCR 12-tubes-stripes 0.2 ml
yes
and 12-cap stripes (domed caps)
Colour
Pack type Pack size
Sales unit
natural
2 bags
400/400
3200 tubes
natural
2 bags
400/400
3200 tubes
natural
2 bags
400/400
4800 tubes
natural
2 bags
400/400
4800 tubes
* Suitable racks for AHN PCR-stripes you will find on page 35 !
AHN Biotechnologie Catalogue 2009/2010
Page 28
5.4 qPCR-Capillaries
Safer, easier, cheaper qPCR
Free of human DNA, certified free of DNase,
RNase and PCR inhibitors
No precipitates
Plastic capillaries from AHN Biotechnologie
do not require the use of BSA in the reaction
mix. In this way precipitates, that can disturb
the optical detection, are avoided.
More optimal reaction
The reaction characteristics are more
optimal as PCR components like TAQ
polymerase, MGCl2, primers, probes and
target DNA do not adhere to the optical
plastic in the way they adhere to glass. The
result is more active components in solution
and therefore a more efficient PCR, using
less target DNA.
Non breaking plastic capillaries 50 l
AHN Biotechnologie has developed a proprietary
solution to frequently breaking Real-Time PCR glass
capillaries. When using these optical plastic capillaries
you avoid the risk of breaking, contamination and injury.
Higher reaction volume
Because of the special design of the tubes,
they can hold up to 50 l reaction mix, which
allows you to add more sample DNA, and
therefore allows you to increase the
sensitivity. This is especially very useful
when detecting low copy numbers of DNA.
Post reaction sample recovery
The special design of the innovative AHN
qPCR-capillaries allows you also to recover
sample after Real-Time PCR with a 10 l
pipette.
Rotor-Adapter for best alignment
Move from glass to plastic
capillaries !!
AHN qPCR capillaries demonstrate excellent chemistry resistance and standards of purity!
Cat.no.
3-130-10-0
3-130-96-0
3-130-SK-0
3-130-SK-1
3-130-RO-0
3-130-RO-0
Description
qPCR-capillaries, caps, transferpin
qPCR-capillaries, caps, transferpin
qPCR-capillaries with rotor adapter 1
qPCR-capillaries with rotor adapter 2
Rotor adapter for light cycler I
Rotor adapter for light cycler II
AHN Biotechnologie Catalogue 2009/2010
Sterile
non
non
non
non
non
non
Colour
natural
natural
natural
natural
natural
natural
Pack type P. size
bag
100
rack-96
1000
rack-96
start kit
rack-96
start kit
carton
1
carton
1
Sales unit
1 x 100
10 x rack-96
7xrack/1 rotor
7xrack/1 rotor
1 rotor
10 x 1000
Page 29
5.5 qPCR-Capillaries
Users guides for qPCRcapillaries
AHN qPCR-Capillaries ® User Guide
Sample preparation / PCR mixture preparation
Samples for PCR can be handled and prepared according to the existing protocol at customer site.
No changes have to be made using AHN polycarbonate capillaries instead of the original glass
capillaries. No changes have to be made preparing the PCR Mix.
It is possible to use AHN polycarbonate capillaries for PCR volumes from 10 µl to 50 µl without
significant changes in the PCR modes (for details see point PCR mode).
Handling capillaries and caps
For better/easier handling of our capillaries and caps we provide a “Transfer-Pin” for taking the
capillaries and the caps out of the rack-box. The Transfer-Pin is part of each sales unit. If you are
used to work without a “Transfer-Pin”, you should use protective gloves to protect you PCRreaction from contamination like DNAse.
Avoid wetting the rim when pipetting the sample into the capillaries. If the rim of the
capillaries is wetted the closure of the caps could be a problem. Therefore pipet direct and
straight in the capillary. For sealing the capillaries with the caps, please use the transfer pin, to
transfer caps from the rack-box to the polycarbonate capillary. Ensure that each capillary is closed
tightly by checking it visually.
Performing PCR / PCR mode (protocol)
Our AHN polycarbonate capillaries have a bigger diameter, compared to the 20 µl glass capillaries.
To guarantee effective heat transfer from the outside of the capillaries to the middle of the PCR
reaction, despite the bigger diameter, it might be necessary to increase the “hold-times” at
denaturing and annealing. For this reason we recommend to program your Cycler mode with
longer hold times for the denaturing and annealing steps. We suggest to program at least 7
seconds at 95°C for the denaturing step and 5 seconds for the annealing step. To short denaturing
and annealing times will lead to a complete failure of your PCR, or at least to much higher CTtimes.
If you are already using a mode with both times higher than 7 seconds, no changes have to be
made normally. Sometimes it might be better to increase the existing times by 2 seconds each.
As the effectiveness of denaturing is also depending on the GC-content of your DNA, it might be
even better to prolong denaturing up to 10 seconds (note: only in case of no PCR signal with a
denaturing time of 5 seconds).
Sample recovery after PCR
Some customer wants to analyse the PCR reaction on a gel. For that purpose the cap can be
taken from the capillary. The recovery of the sample is principally possible using pipettes with a 10
µl tip. In some cases the pipet tips are too broad. In this case you can open the capillaries and spin
the solution into a clean reaction tube.
AHN Biotechnologie Catalogue 2009/2010
Page 30