From www.bloodjournal.org by guest on June 17, 2017. For personal use only.
Tabular
and
Ultrastructural
of the Thoracic
By
Wit/i
the
F.
ALvIN
technical
WEBER
assistance
TUDIES
OF THE
calf were
undertaken
interpretations
of
munized
calves
concerns
an
cytes
attempt
to
discussion
and
OF
of the thoracic
duct
of the
information
to be used
in
in
of
cattle.
of the
effluent,
purposes,
experimentally
leukemic
ultrastructure
duct
Frommes
LITERATURE
and
the
Stephen
observed
preleukemic
thoracic
and
various
to provide
cell
these
types
im-
This
report
agranuloa provisional
according
to unique
characteristics.
There
is little
information
in
agranubocytes
in
lymph
of
any
agranubocytes
in
lymph
of
cattle.
hire
Smith
agranubocytes#{176}
in
JOEL
EFFLUENT
background
characterize
in the
for
structural
in
as
DARREL
REVIEW
CELLULAR
to provide
well
encountered
categorization,
AND
changes
as
AND
of Joyce
INTEoi)UcI’IoN
S
Studies
of Agranulocytes
Duct of Calves
of
cells
lymphocytes
of
structure
Uehershar
in human
thoracic
in
amid
hepatic
peripheral
literature
none
lymph,
cells
tissue
and
healthy
cows
of
the
ultrastruc-
et
al.2
compared
reported
and
of
knowledge
on
Arturson
Knocke,4
of normal
from
ultrastructure
writers’
reported
lymph.
blood
the
to the
Zucker-Frankli&
duct
lymphatic
concerning
and
intestinal
white
studied
the
species,
on
leukemic
and those
the
ultra-
cows,
and
with
tumor-
01_is leukosis.
MATERIALS
Twelve
pumrc1i.ise
from
method
of Joel
thoracic
2 per
f/me
of
National
of
was
Aug.
Laboratory,
Department
of
Minnesota,
St.
#{176}The
term
cell
for
a 3 day
light
for
collected
fixation.
either
Veterinary
Anatomy,
Medical
by
the
(GR-07009)
postoperative
Kg..
and
electron
Palade’s
fixative
with
with
Universiti,
Atonmic
and
of
Energy
of
stumdies.
sucrose7
111g. of
the
samples
For
each
4 ml. of
or
1 : 1 ratio
sucrose
St.
Upton,
per
of
of
ml.
Paul,
Minn.,
( 11-1
)-910),
N. Y.
Commission
Cancer
The
after
contained
Minnesota,
Center,
American
a series
which
45
used.
weeks
antigenic
environment.
surgically.
umsing
microscopic
tube
cacodylate
Research
were
3-4
period.
in a centrifuge
0. 1 NI sodium
supported
50-100
1iP1)roxim1111tel’
then1
with
respect
to their
flow connections
were
prepared
Laboratories,
Grant
26,
Pim.D.,
JOEL.
National
weighing
(AT
Society
In.stitutional
Grant,
it! in nesota.
submitted
DAnIIEI.
poietic
to
sexes.
environnient
following
was
For
METHODS
1)0th
collected
fixative.
Microscope
Universitt,
First
were
niaterial
tetroxi(le
investigation
Electron
and
Department
Brookhaven
This
)
osmium
of
constant
to stabilize
venous
return
effluent
C.
a
Sautter.”
of effluent
( 0-4
cent
From
and
and
(lumct
8 nil.
precoole(l
in
the abbatoir
fistimlas
with
(Iuict
sample.
calves
nmaintained
Thoracic
of
Holstein
iiormal
were
calves
AND
1.965;
Upton,
Veterinary
Paul,
accepted
D.V.M.:
for
Assistant
N.
F.
1. ALVIN
Anatomy,
publication
Scientist,
WEBER,
College
of
Nov.
5, 1.965.
Medical
Research
Center,
PH.D.,
D.V.M.:
Professor
Veterinary
Medicine,
Brookhaven
and
Head,
University
of
Minn.
“agranulocvtes”
is used
here
to exchmde
the
granumlocytic
forms
of the
hemo-
series.
266
Bi.oon,
Voi..
28,
No.
2 (AUGusT).
1966
From www.bloodjournal.org by guest on June 17, 2017. For personal use only.
A(;RANUL0CYTES
OF
THE
1.-Tabular
Table
Calf.
THORACIC
Studies
Types
DUCF
OF
of Agranulocytes
of Agranulocytes
and
Percentage
of Lymphocytes
267
CALVES
of the
Thoracic
Percentage
Showing
of Cells
Nuclear
Duct
Effluent
in each
Bodies
Category;
in the
III
Lymphocytes
Nuclear
I
Lymphocyte
Animal
No.
V
Reticular
Lymphocyte
IV
Proplasmacyte
Mitotic
Forms
(‘/)
(%)
(%)
(%)
(%)
(%)
I)B-2
66.0
17.1
5.5
8.0
1.0
I)B-5
88.0
15.0
2.5
19.5
3.0
1)B-6
94.0
10.1
1.0
1.5
I)B-9
85.0
10.0
2.0
l)B-10
88.0
14.2
1.5
I)B-16
92.0
15.5
1)B-20
96.8
l)B-21
I)B-23
5.0
1.0
4.5
1.5
8.0
2.0
0.5
5.0
4.0
1.5
1.0
4.0
2.0
1.0
11.0
0.0
1.5
0.0
97.0
8.5
0.0
0.8
1.8
2.0
0.3
90.8
11.0
0.0
4.3
4.5
0.5
1)B-24
82.0
15.2
2.5
7.8
7.8
0.0
I)B-35
93.8
7.0
0.0
3.5
2.5
0.3
1)B-42
94.3
8.0
0.3
2.5
2.8
0.3
Range
66-97
Ave.
(Sx)
fixative
soft
were
placed
in
minutes
1-2
small
clumps
Blumil
and
1 hour
water
) . acetone.
of
cells
0.8-1.5
were
then
subsequently
into
1.5-8.0
of
tabular
studies
(1-2.()
and
of
imi a
reticular
made
1 ) . For
)
A
.
This
in previous
400
included
grids
hour.
A.9
The
or
lead
were
categor-
were
counted
cells
in
a count
Ultrastructural
ultrastructural
the
Porter-
microscope.
was
included
the cell
center.
vork1’
on
1/2_i
observed
studies.
cell
near
15
The
mesh
mixture
types
these
cut
300
for
electron
cell
alcohol.
acetone.
were
acetate
a
pellet
dehydrated
and
monoxide
EMU-3F
different
(liameters
at a position
since
lead
RCA
simimltaneommslv.
bodies,
discussion.
report
comiiplement
used
of
this
5600
then
on
form
the
absolute
mounted
uranyl
to
from
0.2
±
latter
as
were
only
well
noted
as
to have
in lymphocytes.
ptmrposes
in
an
the
to he cut
were
saturated
( Table
and
sections
knives.
0.7
2 minutes
were
cent,
Thin
glass
Karnovsky’s
with
studies
appeared
cells
with
survey.
( approximately
characteristics
For
Maraglas.8
with
examined
tabular
of nuclear
per
0.7
separated
aggregates
90
±
reducer-acetone.
with
stained
microscope
these
in
stained
minutes.
P
aninial
at tal)
when
its thin section
studies
initially
electron
for
80.
4.0
for
were
cellular
70.
1.4
slowly
material
The
60.
embedded
an
groups
centrifuged
cellular
minutes.
( 50.
4.8 ±
0.5
permuanganate-acetone.
1-5
five
±
were
of
ultramicrotomes
sections
for
1.3
cells
15
then
and
unique
for
were
support.
Following
1.0
alcohol
Fernandez-Moran
citrate14m
±
clumps
Pta55iu113
film
used
0-5.5
the
sized
of ethyl
without
ized
12
mnn.3
distilled
series
each
2.5
After
Sniall.
a graded
iii
89 ±
used.
Pellet.
an(l
7-17.1
&
SE.
is
II
Plasmacyte
with
Bodies
to
free
the
(lenote
ribosomes.
general
sense
comnplement.
“reticular
immature
and
little
to
rather
terni
the
endoplasmic
describe
than
lymphocyte,”
cells
described
at
defined
form
light
the
The
with
by
which
reticulum.
immature
as
as
agranulocytic
a
Sundberg.12
contained
term
large.
is
a
large
“proplasmacyte”
rough
endoplasmic
level
of magnifica-
microscopic
tion.1 :
Tabular
were
\ounger
than
also
and
made
in
cells
cross-sectional
of
all
agranulocxtes
those
ticall’
selected
oi)viously
illustrations
I)resent
small.1
at
have
in
mature
peripheral
of human
and
to
been
found
studied
sections
monocvtes
studies
aid
to
forms.2’4.15
for
were
and
in
this
of mitochondria.
cell
contain
while
of 20 calf
Mitochondria
random
area
categories
identification.
larger
mitochonciria
agranulocytes
purpose.
excluded
lymphocytes
and
Those
from
the
in the
planimetric
This
work
more
in
of each
using
numerous
mitochondrial
studies.
report
of
are
five
clone
since
mitochondria
monocytes
of the
methods.
was
cell
profiles
The
mitochondria
Low
and
characteris-
groups
were
which
were
Freeman
in
the
were
From www.bloodjournal.org by guest on June 17, 2017. For personal use only.
268
WEBER
Table
2.-Cross-Sectional
Area
Values
for
in the Human
Agranulocyte
and Ox
Mitochondrial
Average
No.
of Cells
Studied
Type
Cell
JOEL
Profiles
No.
Profiles
Cell
AND
per
Mitohondrial
Section
ProfIle
Size
()
on1
1.
3
lymphocyte
2.
3
illOnocyte
7.6
21.3
0.152
±
0.013
0.052
±
0.003
Calf
I.
20
lymphocyte
6.2
0.209
±
0.012
2.
:3.
20
20
I)lasillacYte
reticular
6.7
8.2
0.239
0. 154
±
±
0.014
0.012
4.
20
ProPlasniacYte
7.8
0.201
±
0.011
5.
20
mitotic
7.1
0.150
±
0.008
1\Ieastmrenwmits
nia(le
Microscopic
Co.,
Atlas
forms
lymphocytcs
froill
of
lymphocyte
Normal
and
and
Leukemic
monocytes
Hunian
in
Bloxl.
Low
New
and
Freeman:
York.
Electron
McGraw-Hill
Book
Inc.. 1958.
0 Standard
also
error.
studied
to
negatives
and
(liamneters
ail(l
values
compare
primits
were
between
man
projeCted
in(asurelntmts
at
were
made
a
and
the
final
fronl
ox.
For
magnification
tracings.
These
the
planimetric
of
approximately
data
are
studies
all
150,0()0
l)resentecl
in Table
2.
REsULTS
1 A Description
of Cell
.
Tabular
vith
of amiimals
scopic
into
as
mostly
as
vhich
into
large,
localized.
coarse,
of
endoplasmic
observed
chondria
illustrated
appeared
in the
mitochoncirial
One
instance
an
the
were
other
tribohed.
usually
Iii
are
the
cell
electron
types
opaque
1
).
No
micro-
presence
of
spectrum
of
present
with
near
peripherally
as
or
of free
the
ribosomes.
A small,
was
reniform
commonly
type
unclear
The
smooth-surfaced
nearby,
the
a relatively
background.
rough
centrioles
lymphocytes
mostly
chromatin
numbers
two
(Fig.
1). In
was arranged
appeared
little
very
or
were
and
opaque
sparse
one
descril)ed
multivesicular
in a
total,
since
size-range
which
present
contained
usually
disclosed
of
nucleus
indentation.
to he of similar
size and number
as those
work
of Low
and
Freeman.
The
results
studies
to several
and
electron
sectiomis.
made,
to reniform
nucleus
The
chromatin
a round
masses
with
that
of the
( Table
cent
was
a continuous
or
in
and
was
lymphocytes
cent
in morphology.
was
apparatus,
90 per
observations
nucleolus
area
apparatus
average
and
irregular-shaped
one
in thin
Golgi
of 89 per
bibbed
were
lymphocytes
Golgi
an
large
contained
reticulum,
discrete
and
and
similar
nuclei
translucent
cytoplasm
over
microscopic
essentially
Usually
electron
no
electron
a lymphocyte
instances
some
cent
indicated
encountered
constituted
lymphocytes,
‘ere
Typically
cytes
per
medium,
medium-sized
cells
agranubocytes
lymphocyte
small,
‘ell
various
the
of 66-97
a range
breakdown
the
of the
studies
majority
Types
of
of
Mito-
lymphocytes
quantitative
below.
bodies
discussed
bodies
were
observed
below,
observed
including
in the
per
section
the
mitotic
cytoplasm
in lymphoforms.
of lympho-
In
From www.bloodjournal.org by guest on June 17, 2017. For personal use only.
ACRANULOCYTES
OF
TI-IO1IACIC
TIlE
DUCT
OF
269
CALVES
-
.
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.
.
..
.
Fig.
1.-Lymphocyte.
tus and
centriole.
One
which
cvtes
i ii
cytoplasmic
not
In’ other
duct
peripherally
and
size,
plasmic
and
their
structures
cellular
an
cavities.
cumscribed
omi the
nuclear
( Fig.
in a few
Cobgi
appara-
forms
granular
lymphocytes1
encountered
in the
cvtoplasmic
protrusions
average
2
chromatin
)
.
were
apparatus
1.3
coiii1)rised
by
characterized
masses,
cvtoplasmic
instances
Nucleoli
Gobgi
:.
-
Note
seen
The
microscopic
in normal
cells
effluent.
extensive
cisternae
except
observed
‘
I)ocl\.
azurophilic
light
with
.;‘.
( arrows
peculiar
finger-like
which
located
and
ergastoplasmli
the
in any
..
nuclear
=
and
stimdies
in mono-
reported
similar
here.
to
those
authors
plasmnacyte,
tbioracic
to
NB
h’mphocvtes
comitaine(l
inemitioned
of
corrcspomxl
observed
Lymphocytes
nmmcleobus;
=
sparsit\
i)ovine
fibrillar
were
The
Note
oulcl
occasionally
cytes’5
Nim
The
cisternae
electron
not
was
which
component
opaque
observed
usually
were
material
in plasmacyte
observed.
cemit
( ()-5.5
presence
were
of
usually
p
the
)
of
umform
of the
coarse,
in
shape
rough-surfaced
ergasto-
not
dilated,
was
extensively
miot obvious
miuclei.
Cytoplasmic
within
A small,
protrusions
cir-
From www.bloodjournal.org by guest on June 17, 2017. For personal use only.
WEBER
270
AND
JOEL
,,g.’..
IZ
Er
:::
4s
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i
;.IP
#{231}5q
‘.
.s
#{149}1
5’ ..i
.,
..
.
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)‘
ft.
‘F
..
Fig.
A
2.-Plasmacyte.
few
‘ere
small
(olgi
cell
iii
type,
purposes,
varied
other
round,
more
clumps
( Fig.
which
that
3
)
lymph
which
occasionally
cytoplasm
were
similar
( 0.8-19.5
cell
‘as
appeared
Golgi
per
in
observed
)
cent
apparatus
usually
descriptive
animals
the
than
and
one
The
rough
amount.
was
present
of
chromatin
to
several
abundant,
in
any
presence
irregular-shaped
form,
sparse
for
among
by
contained
very
in lymphocytes.
hemocytoblast
described
by
recipients.
The
numbers
of
cent
of total
cells
counted,
configuration.
were
border
lymphocyte’2
small,
strand
I)ocly’.
of the nmmcleus.
multivesicular
=
left
characterized
with
characteristically
in polyribosomal
inconspicuous
to those
a reticular
nucleus
componemits
the upper
similarities
to the
nodes
of homograft
averaged
4.8 per
1 ) . This
reniform
arrow
cisterna;
near
called
in occurremice
or
reticular
and
is herein
( Table
The
often
plasmic
small
.
in
videly
cell type
oblong,
ergastoplasmic
remarkable
Andr#{233}-Schwartz1”
reticular
lymphocytes,
.c
4.
. ,
fimiger-like,
and
size
which
showed
=
.
can be ohsemvecl
vesicles
irregular
The
Er
.
nucleoli
free
and
ribosomes,
smooth
A discrete
near
the
endothough
nuclear
a
From www.bloodjournal.org by guest on June 17, 2017. For personal use only.
AGIIANULOCYTES
OF
THE
TIIORACIC
DUCT
OF
CALVES
271
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.
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Fig.
The
3.-Reticular
large
Note
central
the
(G )
i\-l
.
abundance
imidentation.
I)lunt
The
l)y
those
the
presemice
several
miucleoli
scattered,
absence
was
was
of free
-.-
scattered
inconspicuous
usually
mnuch
approximately
( Table
cent
reniform
an
Golgi
shorter
4 per
cent
cell
was
1 ). This
nucleus
electron
with
translucent
apparatus
and
more
sparse,
omi the average
were
present
within
by
A prominent,
indentatiomi.
the
presence
reticular
circumscribed
Cytoplasmic
of cells studcharacterized
small,
irregular-
background,
were
endoplasmic
nuclear
masses.
characteristics.
body.
were
characterized
ribosomes.
in the
to
chromatin
nucleolar
types.
per
iii
nuclear
definite
multivesicular
4 ) . The latter
they
frequently
rough-surfaced,
present
small
constituted
a round
clumps
since
cytoplasm
cell
form
( Fig.
mcomispicuous,
The
other
of 1.5-8
of
chromatin
shows
protrusions
of the
a variatiomi
shaped
the
.,
,.
,
proplasmacyte
with
Note
arrow
Cytoplasmic
than
ied,
lymphocyte.
.
electron
opaque
mass ( Nim ) shows
of free ribosomnes
(H ) the rather
rnitochondria;
=
‘:‘
-
of
cisternae,
and
snialb,
and
chromatin
one
a variable
amount
and usually
also
Golgi
protrusions
apparatus
were
to
also were
masses.
of
the
usually
shorter
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WEBER
272
Fig.
4.-Proplasmacyte.
two
nucleus
apparatus
the
probably
and
are
section
and
more
cells
)
undergoing
of all
acteristics
was
in
cells
some
while
numerous
chromatin
masses
and
lower
edge of the
a nucleolus.
Note the promiiinemit
Colgi
(Er)
It is fortuitous
and unusual
that
7.8 miiitochonclrial
profiles
were commuted
of
mass
the
near
.
of
text).
thus
mitosis,
studied
( Table
they
belonged
indicated
5 and
6
)
.
No
studies
most
represemited
bum,
and
edge
average
an
nmmcbear
JOEL
‘ere
less
conspicuous
than
those
present
in
or plasmactes.
The
( Figs.
cut
(see
type
scattered
opaque
cisterna
since
attemitmated,
lymphocytes
( 0-2.0
the
absent
smiiabl,
electron
ergastoplasmiiic
this cell
iii
the
) . Another
represents
(C)
mmtochonclria
per
Note
( Nim
nucleoli
AND
imistances,
in
free
were
ribosomes.
1
it
)
comprised
,
to all
of the
determine
the
cytoplasm
of
rough-surfaced
devoid
cells
undergoing
average
of
these
four
cells
organelle
mitosis
cent
chardescribed
of the
where
groups
contained,
endoplasmic
this
per
cytoplasmic
previously
which
of
of 0.7
whose
types
Thus,
essentially
Imi those
four
an
cells
to
complement
was
contained
made
frequently.
a barge
others
which
reticuor
contained
cytoplasmic
From www.bloodjournal.org by guest on June 17, 2017. For personal use only.
AGIIANULOCVTES
OF
FIlE
TIIOIIACIC
OF
l)UC1
.U
273
CALVES
S
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Fig.
5.-1itotic
late
miiatter
free
is
not
ril)OSOIlieS.
\y(q(
sptrst’,
chromiiosornal
BOG1!,
Nuclear
liiid
to
usualbv
an
type.
lvmphocvtic
opaque
it appears
iiiconiplete
sas
eii(IOI)lasifl
crc
( Fig.
imi detail,tm
imi other
a smaller
of the
electron
include
=
1)articu-
substantial
numbers
agranulam
of
Cur
cvtoplasniic
of
reticular
niein-
( Fig.
bod’
electron
(0.2-0.3
ic reticulum
profile
elongate
bocated
at
the
or
two
of
of
periphery
5).
Studies
l)oclies
scril)ed
cattle
there
COfli1)IeIiiellt
lVfli1)bioc\’tcs
than
although
h’ smooth-surface(l
2. Nuclear
small,
peri1)heral
the
to be
appears
The
)
COITIpOiiemlts
essential
the
idemstifiable
Note
( End
bramies
form.
This cell
niitochoimclria;
SI
chromiiosoine;
-
observed
1, Table
exclusively
1
).
These
ol)serVedl
m.:u
cells
of the
opjmme
vs. 0.4-4).6
ox
cemitrab
/.L dia.).
to
in
cells
of
structures,
which
l)(i slightly
smaller
(0.6-0.8
vs.
0.8-1.2
portion
than
In some
instances
j.t.).
group
in
also
contained
other
cells
portion
the
de-
diameter
in
central
is,
were
overall
They
observed
the
1-that
previously
of
of the
From www.bloodjournal.org by guest on June 17, 2017. For personal use only.
WEBEII
274
Fig.
6.-Mitotic
form.
M
chromosome;
This
cell
appears
miiitochonclriomi;
=
to
Er
be
of
the
plasmacytic
ergastoplasmic
=
AND
type.
cisterna;
C
=
JOEL
Chr
=
Gobgi
apparatmms.
body
nuclear
part
was
all
instances
for
of
studies
that
showed
cent
of
cells
Quantitative
Visual
files
even
of
though
lymphocytes.
associated
cattle.
They
the
Nuclear
with
corresponded
per
of
usually
usually
peripheral,
bodies
nucleoli,
as
were
approximately
occurrence
was
section
fibamentous
were
was
in
essentially
commonly
located
observed
within
to two-thirds
(Table
ier
the
nuclear
nucleus,
( Table
them
occurrence
of the
of
present
an
of the
inner-
diameter
1
).
i)Ody
and
There
that
were
in
lymphocytes
approximately
no
12
apparent
sex
or structure.
Mitochondrial
inspection
cell
the
one
contained
in their
differences
3.
for
nucleus.
Tabular
per
cells
which
lacking,
size
closely
l)ody
area
of the
essentially
usual
not
other
nuclear
was
the
Studies
data
2)
concerning
indicated
the
that
number
values
of
for
mitochondrial
agranulocytes
proof
the
From www.bloodjournal.org by guest on June 17, 2017. For personal use only.
ACIiANULOCYTES
OF
THE
THOIIACIC
DUCt’
OF
275
CALVES
and calf are of similar
magnitude
(7.6 vs.
The number
of mitochondrial
profiles
per cell section
( 21.3 per cell, av. ) was found
to be much
larger
than
6.2 per cell, respectively).
for the human
monocyte
human’6
five
groups
studied
tively
) . The latter
to be
since
calf
in the
average
of a smgle
population.
differences
in
Statistical
cell
cytes,
3
tively
analyses
sizes-between
agranulocytes-and
the
inadequate
)
monocytes
for
any
calf (6.2, 6.7, 8.2, 7.8, and 7.1
values
for the five cell groups
would
appear
to
of these
calf
human
and
data
cells
were
human,
agranulocyte
render
of the
not
and
statistical
made,
among
(3
numbers
any
in the
per cell, av., respecin the calf appeared
the
lympho-
treatment
rela-
meaningless.
Analyses
of data
the
various
classes
well
as
human
exist
profile
among
sizes
the
average
from
planimetric
between
of
were
highly
significantly
Low
cytic
cells
that
these
cells
those
of the
no
(Table
human
smaller
(0.05
value
was
)
cells
observed
than
of
those
the
size
below
0.08
in
calf
as
differences
all mitochondriab
measured
on
the
studied
from
the
profiles
which
of all other
calf
sizes
of the
significant
which
were
mitochondrial
av.
.c2,
profile
effluent
2). Thus,
monocyte,
monocytes,
Freeman,1#{176}
contained
In all agranulocytic
profile
duct
The
j.2.
and
observed.
mitochondrial
thoracic
indicate
in
0.15-0.25
illustrations
of
in the
lymphocyte16
mitochondria
studied,
except
rnitochondrial
studies
of agranulocytes
studied,
agranulo-
no
individual
j..
DIscussioN
As the
result
is assumed
that
light
thoracic
duct
microscopic
studies
effluent
consists
on animal
of 90-95
The ultrastructural
studies
of Zucker-Framikbinm
duct effluent
amid those
of Arturson
et al.2 on
cytes.1
racic
intestinal
cyte
lymph
is not
for
of many
this
in
readily
is that
flattened
as
smear
dogs,
studies
in
work,
material
that
thoracic
as vell
duct
lymph
here
are
of
results
the
of
the
control,
light
in
calf
are
lympho-
One
the
of others
and
hepatic
small
preparations.
encountered
lympho-
of human-tho-
technics,
From
as the
cells
that
microscopic
cells
on
it generally
small
lymphocytes
thin-section
those
cent
indicate
preparations.
smear
assume
of
our
microscopic
of our
to
PreI)arations
case
as
in electron
identifiable
in ebectromi
is the
reasonable
well
as
lymph,
per
for
reason
cells
are
not
microscopic
sheep,21’22
electron
it is
microscopic
primarily
medium-sized
lymphocytes.
If the
cytes
studies
are
reported
present
in
the
that bovine
monocytes
human.
A complicating
era!
blood
called
contain
factor
contains
duct
small
is that
The
monocyte
only
question
of the
mitochondria
it commonly
atypical
occasionally
of the effluent
of lymph
iii
the thoracic
duct
cytes
observed
to whether
as
calf,
it must
of the size
is accepted
monocytes,
up
to 20 per
cent
has
vessels.2
effluent
amid frequent
our
studies
it
originally
was
been
reported
Winqvist2
of one of
monocytes
popliteal
nodes,
presumably
none in the efferent
flow from
In
the
effluent
mono-
be
assumed
described
that the
forms
for the
periph-
which
are
monocytoid-lymphocytes.
uent
the
but
of cattle
to answer
thoracic
and
after
these
macrophages
stimulation
nodes.
believed
as a usual
observed
six calves.
with
that
cellular
in afferent
human
monocytes
constit-
0.1 per cent monoHall
and
Morris22
vessels
serum
and
globulin,
monocytoid-
to
From www.bloodjournal.org by guest on June 17, 2017. For personal use only.
276
WEBE1I
lymphocytes
were
forms
evident,
however,
phocytes.
present.24
which
granular
and
Studies
sections
of
lymphocytes
it
of
the
cells
in
are
mitochondrial
are
logical
the
cells
these
areas
values
of
(0.052
s)
are
made,
the
the
that
human.
to
Until
quantitative
as
calf
lym-
similar
and
question
of
as
showed
the
level
effluent
sufficiently
cells
a size
of
ultrastructural
duct
thoracic
not
classify
mitochondria
monocytes
at
these
in
to
JOEL
membrane-bound
were
profile
colitailied
thali
and
monocytes2
average
rather
of
presemit
reticulum
of
appeared
identified
studies
monocytes
thus
question
)
momiocytes
endoplasmic
characteristic
mitochondriab
(0. 152
bovine
The
are
ANI)
to
must
whether
remain
umi-
amiswered.
Generally
ported
speakimig,
as
cytes
imi the
cells
presemit
Our
imi this
finding
Immaturc-
fri
the
calf
a given
two
were
1)reselit
the
The
regional
peared
injection
in the
hours
the
efferemit
of
liumami
agaimi
of
the
iiotecl
characteristics
the
of
graft
reaction
clear
characteristics
siolially
such
in
to assigmiing
or
two
cells
to attemiif)t
level
thami
cyte”
was
imseci
miuclei
of
these
which
indicated
proper
in
a nondescript
was
to use
at
P055il)le
rather
cells
a fairly
designations
blood
than
the
either
light
juvenile
were
small
form.
selected.
and
homeostatic
hours
of
agranulocytes
this
area.
ap-
By
120-150
lymphocytes.
It
occur
in
of lymph,24
to
ctoplasmnic
adult
cattle.2
is
calves,
may
The
lymphocyte
“immature
cells.”
terrniiiobogy
at
level.
or “immature
chromatimi
primary
or
It
the
The
question
amid
nu-
amid
occa-
of
terms
preferable
pbasmacyte
would
seem
ultrastructural
term
plasmacyte”
clumps
homo-
are
use
to be
of
many
set
types
appeared
the
have
a first
basopb#{236}ilia
plasmacytic
microscopic
The
to
observed
and
to
as
definitive
“plasmacyte”
contained
of
such
mnore
one
lymphocytes
30-50
occasionally
composition
“proplasmacyte”
question
response
in
provide
a response
vith
category
cellular
within
from
was
as
bymphocytic
and
adult.122
additionally,
that
medium-sized
appeared
peripheral
the
be
lymph
cells.
Agranubocytes
imi the
of
immature
nodes
may
of
and,
to
than
proplas-
lymph,
reticular
that
lymiipliocyte
immature
these
effluent
studies
respect
taken
which
may
the
cellular
which
lymphocyte”
the
to
appropriate
the
of
“reticular
groups,
cells
this
cells
of
globulin,
of these
in
a greater
this
showed
mainly
reticimlar
rabbits.1’
ol)servedl
as
the
of
and
animals,
numbers
popliteal
emiteritis,
to alter
re-
plasma-
cent
duct
with
forth
iii
were
contained
production
previommslv,
deal
call
Morris22
the
yet
young
ili
gamma
l)een
no
per
microscopic
they
may
not
lymphocyte
as
noteworthy
amid
lymph
effluemit
stimulated
As
and
prevalent
0.2
in thoracic
electron
precautioiis
hall
found
reticular
greater
of
possil)le
that
a subclinical
and
vbiich
has l)een
Doted
have
is
though
work
but
described
stimulus
It
have
reported
goats.
of
few
substantially
evemi
conclitiomis.
been
re1)Orted,
antigenic
calves,
and
more
animals.
calves,
of three
Very
be
precursors
Winqvist2m
of plasmacytes
not
heeii
may
six
studies,
factors.
have
mature
possil)ly
numbers
have
forms
in
one
several
effluent
2:1
of
from
which
to
vascular
lymph
simnilar
their
lymph.1222
duct
in
forms,
amid
normal
effluent
of larger
show’n
attril)utal)le
than
of
thoracic
previously
macyte
pbasmacytes
constituents
“proplasmabecause
the
several
nucleoli
is whether
or
not
From www.bloodjournal.org by guest on June 17, 2017. For personal use only.
AGRANULOCYTES
The
OF
presence
of
disassociation
why
is
reVious
work
the
small
nucleus
calf
is
l)Odies,
for
sphere
whose
omi
the
al)out
1/6,
or
would
appear
is
of
per
that
imicluded
on
of
inner
some
of
this
in
basis
of
diameter
The
l)Odies
of
in
observed
may
gone
of
are
in
occur-
a 4 ji
sphere
of
12
per
especially
cent
when
unobserved,
have
a
lymphocytes
figure
may
nuclear
within
singular
value,
have
the
located
sections
theoretical
lymphocytes
corresponded
he
and
thin
effluent
of
nuclear
Thus
bodies
in
to the
which
to
iiuclear
time.
close
category
the
body,2#{176} should
duct
sections,
nucleus.
estimated
observed
the
nuclear
imi the
be
microns
be
area
of the
Since
0.7
cent
nuclear
can
reasonal)ly
their
unanswered
which
constituent
diameter
calculation,
theoretically
17
considered
an
is 4 microns.
to l)e
and
Another
in lymphocytes.
in
approximately
should
lymphocytes,
portion,
active
of the
diameter
they
cells
located
piirpos-s
average
rence,
in
at present.
opaque
more
absent
two-thirds
to
exclusively
average-sized
lymphocyte
of the thoracic
6 microns
in diameter.
In
thin
were
approximately
the
277
CALVES
unexplainable
electron
I)e
or nearly
approximately
usually
b)Odies
to
OF
body
are
central
of the
DUCT
nuclear
nucleoli,
appears
l)e relatively
the
THO1IACIC
the
from
(fuestiomi
The
TIlE
and
been
it
some
immature
or
misclassified.
SUMMARY
I
Ultrastructural
.
thoracic
studies
duct
2.
Tabular
electron
that
demonstrated
per
edit
niitotic
of the
profile
aIld
five
designated
Monocyte
sizes
cell
)
,2
than
types
in
monocytes
of
the
agranulocytes
per
cent
the
present
plasmacytes,
proplasmacytes,
were
and
the
that
similar
and
the
human
profile
among
0.7
1.3
per
cent
sections
of
highly
These
in the
( 6.2-
numbers
cell
lymphocyte
were
cells studied.
are not present
probably
from
enumerated.
calf
of
other
each
sexes.
the
4.8
demonstrated
profiles
of
of both
cent
ji2)
groups
those
per
studies
(0.15-0.25
mitochondrial
Icr (0.05
proof
that
cell
tabular
agranubocytes,
lymphocytes,
4
various
400
calves
evaluation
were
lymphocytes,
Mitochondrial
8.2)
cent
of
Holstein
microscopic
89 per
reticular
forms
3.
made
were
of 12 normal
effluent
of
the
significantly
studies
thoracic
the
human.
smal-
provided
effluent
added
of the
calf.
4.
1)r(’semit
were
to be present
foumid
associated
with
the
practically
nucleolus,
were
mio electron
opaque
SUMMAIIIO
1.
de
tromiic,
iliter1)rendite
Esseva
ab
amiihe
2.
Le
in lymphocytes.
They
were
the average
in 12 per cent of thin sections
of cells in this class.
In
to nuclear
bodies
of other
nonblood
cells,20
in lymphocytes
they
were
contained
veniente
only
011
comitrast
not
bodies
Ntmcbear
he
IN
stiilios
del
effluente
smaller
in overall
central
portion.
and
often
INTERLINCUA
ultrastructural
ducto
diameter,
de
thoracic
de
400
12
agranimbocytos,
normal
omnes
vitelbos
Holstein
sexos.
evaluation
deinonstrava
dcl
tabular
que
89
pro
agranubocytos,
cento
a base
esseva
del
lymphocytos,
microscopia
4,8
deepro
cento
From www.bloodjournal.org by guest on June 17, 2017. For personal use only.
278
WEBER
plasmocytos,
0,7
C
cento
Le
evalutation
3.
de
1,3
pro
profibo
inter
pro
tabular
e Ic
altere
4.
Ibbos
plus
contrasto
in
nuclear
12 pro
p1acticamente
in
nubbe
Ic
br
non
diametros
portion
in
que
esseva
he
illos
que
additional
solmente
sectiones
que
del
vitelbo.
in bymphocytos.
tenue
del cellulas
altere
de
e ilbos
vitelbo
ab
.t2)
thoracic
associate
simile
he
monocytos
prova
nuclear
esseva
numeros
in
(0,05
un
es presente
cento
de
general,
central
de
le efflimente
corpores
illos
cellular
signification
provideva
le
esseva
2)
mitochondriab
studios
con
que
(0,15-0,25
gruppos
de
presente
he bmphocytos
parve
profibos
non
proplasmocytos,
demonstrava
profilo
nivelbo
corpores
cento
JOEL
enumerate.
enumerate
Le
Iste
4 pro
cellular
de
alte
a! media
Per
in
esseva
que
typos
cinque
a
studiate.
troate
classe.
iste
mitochondrios
del
micre
presente
sanguinee,
del
probabilemente
CS
Esseva
esseva
vane
homine.
plus
cellulas
mnomiocytos
in
del
esseva
reticular,
del
niagnitudes
cellular
e be lymphocyto
homine
lymphocytos
mitotic
(6,2-8,2)
be sectiones
dc
cento
formas
AND
con
cellulas
non-
be nucleolo,
illos
frequentemente
contineva
electronicamente
opac.
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1966 28: 266-279
Tabular and Ultrastructural Studies of Agranulocytes of the Thoracic Duct
of Calves
ALVIN F. WEBER, DARREL JOEL, Joyce Smith and Stephen Frommes
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