BONE HISTOLOGY SLIDE PRESENTATION
PRESENTED BY: SKELETECH, INC.
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SkeleTech invites you to use these complimentary images for your own presentations or as
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Slide 1. Cortical Osteons (Harversian system)
Polarized Light (Magnification: 200x)
Slide 2. Cortical Osteons (Harversian system)
Bright Field (Magnification: 200x)
Description:
Slide 1 is an unstained ground section of monkey femur mid-shaft viewed under polarized light. This
slide shows mature BRU (bone remodeling units) when cortical bone remodeling is completed. Note
osteons with lamellar bone arranged in concentric layers and a central Harversian canal (open arrows).
Also note interstitial bone between osteons. (sections highlighted in red) Original magnification 200X.
Slide 2. Same bone section (Slide 1) when viewed under bright field. Note osteocytes arranged in
concentric layers and in parallel with lamellar bone.
Slide 3. Mineralized bone matrix, osteoid &. osteoclasts
Goldner’s Trichrome Stained (Magnification: 100x)
Slide 4. Mineralized bone matrix, osteoid & osteoclasts
Goldner’s Trichrome Stained (Magnification: 200x)
Description:
Slide 3. A section of monkey lumbar vertebral body showing bone matrix not yet mineralized, i.e.,
osteoid (stained red), mineralized bone matrix (stain green). The structure at the arrow is the bone
resorbing osteoclasts. Undecalcified, 5-µm Goldner’s trichrome stained.
Slide 4. Same bone section (Slide 3) at higher magnification.
SkeleTech – Bone Histology Slide Set
2001
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Slide 5. Cortical Bone Remodeling
Toluidine Blue Stained (200x)
Slide 6. Cortical Bone Remodeling
Toluidine Blue Stained (400x)
Description:
Slide 5. A section of monkey femoral neck cortical bone showing cortical bone remodeling in progress
(solid arrows) and several osteons that have completed a remodeling cycle (open arrows). Note the
space created by osteoclastic bone resorption, which is being filled by newly synthesized but not yet
mineralized bone matrix (i.e., osteoid, stained light blue) and osteoblasts on the osteoid surface.
Undecalcified, 7-µm toluidine blue stained.
Slide 6. Same bone section (Slide 5) at higher magnification showing a mature osteon that has
completed a remodeling cycle.
Slide 7. Cortical Bone Remodeling (different field)
Toluidine Blue Stained (200x)
Slide 8. Cortical Bone Remodeling (different field)
Toluidine Blue Stained (400x)
Description:
Slide 7. A section of monkey femoral neck cortical bone showing cortical bone remodeling in progress.
Undecalcified, 7-µm toluidine blue stained.
Slide 8. Same bone section (Slide 7) at higher magnification. The two remodeling sites are in a bone
formation phase with osteoid seams (solid arrow) and osteoblasts (open arrows) clearly visible.
SkeleTech – Bone Histology Slide Set
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Slide 9. Cancellous Osteon.
Toluidine Blue Stained (100x)
Slide 10. Cancellous Osteon.
Toluidine Blue Stained (200x)
Description:
Slide 9. A section of monkey femoral neck showing a cancellous osteon, which represents the end of a
remodeling cycle. Note a packet of lamellar bone deposited on the previous resorbing surface (cement
line-solid arrows). A new remodeling site within the cancellous bone is also shown. Undecalcified, 7-µm
toluidine blue stained.
Slide 10. Same bone section (Slide 9) at higher magnification.
Slide 11. Cancellous Bone Remodeling
Toluidine Blue Stained (100x)
Slide 12. Cancellous Bone Remodeling
Toluidine Blue Stained (200x)
Description:
Slide 11. A section of monkey lumber vertebral body showing active bone remodeling in cancellous
bone. Note osteoclastic bone resorption (resorption phase) at the lower left (solid arrows) and new bone
formation (formation phase) at the upper right (open arrows). Undecalcified, 5-µm toluidine blue stained.
Slide 12. Same bone section (Slide 11) at higher magnification.
SkeleTech – Bone Histology Slide Set
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Slide 13. Osteoclastic Bone Resorption
Goldner’s Trichrome Stained (Magnification: 200x)
Slide 14. Cancellous bone formation
Goldner’s Trichrome Stained (Magnification: 200x)
Description:
Slide 13. A section of monkey lumbar vertebral body showing two cancellous bone osteoclasts (solid
arrows) with less defined resorptive cavities and one larger osteoclast with a prominent resorptive cavity
(Howship’s lacunae- open arrow). Undecalcified, 5-µm Goldner’s trichrome stained. Original
magnification 200X.
Slide 14. A section of monkey lumbar vertebral body showing a layer of closely arranged osteoblasts
(solid arrows) and their newly deposited bone on the cancellous bone surface. A new remodeling site
within the cancellous bone is also shown (open arrow). Undecalcified, 5-µm Golder’s trichrome stained.
Original magnification 200X.
Slide 15. Longitudinal Section of a Monkey Lumbar
Vertebral Body. (Magnification: 10x)
Slide 16. Cross Section of a Monkey Lumbar Vertebral
Body. (Magnification: 10x)
Description:
Slide 15. A section of monkey lumbar vertebral body stained with the von Kossa’s method plus Macneal’s
tetrachrome counterstain. Mineralized bone is stained black and soft tissues are stained light blue.
(Original magnification 10x)
Slide 16 A section of monkey lumbar vertebra body stained with the von Kossa’s method to show
mineralized cortical bone and cancellous bone (black). (Original magnification 10x)
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Slide 17. Calcein labels in cortical bone
Fluorescent Microscopy
Calcein Labels (Magnification: 100x)
Slide 18. Calcein labels in cortical bone
Fluorescent Microscopy
Calcein Labels (Magnification: 200x)
Description:
Slide 17. A section of monkey femoral midshaft cortical bone showing periosteal and intracortical labels
as seen under fluorescence microscopy. Bone marrow is on the right. Unstained 50-µm ground section.
Slide 18. Same bone section (Slide 17) at higher magnification to show periosteal single (solid arrow)
and double labels (open arrow).
Slide 19. Endosteal and intracortical labels
Fluorescent Microscopy
Calcein Labels (Magnification: 100x)
Slide 20. Endosteal and intracortical labels
Fluorescent Microscopy
Calcein Labels (Magnification: 200x)
Description:
Slide 19. A section of monkey femoral midshaft cortical bone showing endosteal and intracortical bone
calcein labels as seen under fluorescence microscopy. Bone marrow is on the upper left. Unstained 50µm ground section. Original magnification 100X.
Slide 20. Same bone section (Slide 24) at higher magnification. Original magnification 200X
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Slide 21. Calcein Labels in Cancellous Bone
Fluorescent Microscopy Calcein Labels
(Magnification: 200x)
Description:
Slide 21. A section of monkey lumbar vertebral body showing single (solid arrow) and double labels
(open arrow). Unstained 8-µm section.
Slide 22. Calcein and Alizarin Red Labels in Cancellous
Bone. Fluorescent Microscopy Calcein and Alizarin Red
Labels (Magnification: 100x)
Description:
Slide 22. A section of monkey femoral neck showing alizarin red labels given first (red, single (yellow
solid arrow) and double (yellow open arrow)) followed by calcein labels (yellowish green, single (white
solid arrow) and double (white open arrow)) in cancellous bone as seen under fluorescent microscopy.
Unstained 10-µm section.
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