User Manual
Dionex ERS 500 Suppressor
031956 Revision 11 • January 2017
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Manual
for
Dionex Anion Electrolytically Regenerated Suppressor 500
(Dionex AERS 500 (4 mm), Item # 082540)
(Dionex AERS 500 (2 mm), Item # 082541)
Dionex Cation Electrolytically Regenerated Suppressor 500
(Dionex CERS 500 (4 mm), Item # 082542)
(Dionex CERS 500 (2 mm), Item # 082543)
Dionex Anion Electrolytically Regenerated Suppressor 500e
(Dionex AERS 500e (4 mm), Item # 302661)
(Dionex AERS 500e (2 mm), Item # 302662)
Dionex Cation Electrolytically Regenerated Suppressor 500e
(Dionex CERS 500e (4 mm), Item # 302663)(Dionex CERS 500e (2 mm), Item # 302664)
Dionex Anion Electrolytically Regenerated Suppressor 500 for
Carbonate Eluents
(Dionex AERS 500 Carbonate (4 mm), Item # 085029)
(Dionex AERS 500 Carbonate (2 mm), Item # 085028)
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 2 of 84
© 2017 Thermo Fisher Scientific Inc. All rights reserved.
MASTERFLEX C/L is a registered trademark of Barnant Company. All other trademarks are the property of
Thermo Fisher Scientific Inc. and its subsidiaries.
Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the product
operation. This document is copyright protected and any reproduction of the whole or any part of this document is
strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.
The contents of this document are subject to change without notice. All technical information in this document is for
reference purposes only. System configurations and specifications in this document supersede all previous
information received by the purchaser.
Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error free and
assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might result from any
use of this document, even if the information in the document is followed properly.
This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This
document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of Sale
shall govern all conflicting information between the two documents.
Revision History:
Revision 08, April 24, 2013, Rebranded for Thermo Scientific. Product name changed from Dionex SRS 300 to
Dionex ERS 500.
Revision 09, November, 2013, Updated External Water Mode flow rate recommendations, Updated hydration
procedure.
Revision 10, August, 2015, Added support for the Neutralization mode. Added support for Carbonate Eluents.
Revision 11, January, 2017, Updated the recommended suppressor hydration procedure. Edited for length and
clarity. Added Dionex ERS 500e product.
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 3 of 84
Safety and Special Notices
Make sure you follow the precautionary statements presented in this guide. The safety and
other special notices appear in boxes.
Safety and special notices include the following:
!
Indicates a potentially hazardous situation which, if not avoided, could result in death or
serious injury.
SAFETY
!
WARNING
!
CAUTION
!
Indicates a potentially hazardous situation which, if not avoided, could result in damage to
equipment.
Indicates a potentially hazardous situation which, if not avoided, may result in minor or
moderate injury. Also used to identify a situation or practice that may seriously damage the
instrument, but will not cause injury.
Indicates information of general interest.
NOTE
IMPORTANT
Tip
Thermo Scientific
031956-11
Highlights information necessary to prevent damage to software, loss of data, or invalid test
results; or might contain information that is critical for optimal performance of the system.
Highlights helpful information that can make a task easier.
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 4 of 84
Contents
Contents
1.
Introduction .........................................................................................................................8
1.1
Electrolytically Regenerated Suppressor .................................................................................... 9
1.2
Overview of Suppression and Neutralization Modes ................................................................ 15
1.2.1
1.2.2
1.2.3
1.2.4
1.2.5
1.2.6
1.3
Mode of Operation Selection ......................................................................................................... 16
The AutoSuppression Recycle Mode ............................................................................................. 16
The AutoSuppression External Water Mode .................................................................................. 17
The Chemical Suppression Mode .................................................................................................. 17
The MPIC Suppression Mode........................................................................................................ 17
The Neutralization Mode ............................................................................................................... 18
Shipment and Storage .............................................................................................................. 19
1.3.1
1.3.2
2.
Shipment ...................................................................................................................................... 19
Storage ......................................................................................................................................... 19
Installation ......................................................................................................................... 20
2.1
System Requirements .............................................................................................................. 20
2.2
Electrolytically Regenerated Suppressor Control ...................................................................... 22
2.2.1
Dionex Reagent-Free Controller (Dionex RFC) ............................................................................. 23
2.3
Dionex SCC-10 Suppressor current controller (Item # 074053) ................................................ 23
2.4
Back Pressure Coils for the Dionex ERS 500, Dionex ERS 500e and
Dionex AERS 500 Carbonate................................................................................................... 24
2.4.1
2.5
Assembly ...................................................................................................................................... 24
Gas Separator Waste Tube for the Dionex ERS 500 ................................................................. 25
2.5.1
2.6
Assembly ...................................................................................................................................... 25
Electrolytically Regenerated Suppressor Current Selection....................................................... 25
2.6.1
3.
Calculating the Optimum Current Setting ...................................................................................... 25
Operation ........................................................................................................................... 28
3.1
Chemical Purity Requirements ................................................................................................. 28
3.1.1
3.1.2
3.1.3
3.2
Inorganic Chemicals...................................................................................................................... 28
Solvents ........................................................................................................................................ 28
Deionized Water ........................................................................................................................... 28
Start-up.................................................................................................................................... 28
3.2.1
3.2.2
3.2.3
3.3
Hydration...................................................................................................................................... 29
Back Pressure Coil Pressure Test ................................................................................................... 30
Quick Back Pressure Check........................................................................................................... 30
Plumbing for the AutoSuppression Recycle Mode Operation ................................................... 31
3.3.1
3.3.2
3.3.3
3.4
Eluent Flow Path Connections in the AutoSuppression Recycle Mode ........................................... 32
Regenerant Flow Path Connections in the AutoSuppression Recycle Mode .................................... 32
Installation in Thermal Chamber ................................................................................................... 32
Plumbing for the AutoSuppression External Water Mode Operation ........................................ 34
3.4.1
3.4.2
Thermo Scientific
031956-11
Eluent Flow Path Connections for the AutoSuppression External Water Mode ............................... 35
Regenerant Flow Path Connections in the AutoSuppression External Water Mode ......................... 35
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 5 of 84
Contents
3.4.3
Regenerant Flow Path Connections in the AutoSuppression External Water Mode
with Peristaltic Pump .................................................................................................................... 36
Installation in Thermal Chamber ................................................................................................... 37
Dionex SRD-10 Suppressor Regenerant Detector........................................................................... 37
3.4.4
3.4.5
3.5
Plumbing for Chemical Suppression Mode Operation .............................................................. 37
3.6
Plumbing for MPIC Suppression Mode of Operation ............................................................... 37
3.6.1
3.6.2
Eluent Flow Path Connections in MPIC Suppression Mode ........................................................... 38
Regenerant Flow Path Connections in MPIC Suppression Mode
Using Pressurized Water Delivery System ..................................................................................... 38
MPIC Suppression Mode Operation .............................................................................................. 38
3.6.3
3.7
Plumbing for Neutralization Mode Operation ........................................................................... 40
3.7.1
3.7.2
3.7.3
3.7.4
3.8
Sample Liquid Line Connections for Dionex ERS 500e in Neutralization Mode ............................. 41
Regenerant Liquid Line Connections for Dionex ERS 500e in Neutralization Mode ....................... 41
Pump Trap Column Installation and Regeneration ......................................................................... 41
Neutralization Mode Operation ..................................................................................................... 42
Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500 Carbonate Storage.......................... 43
3.8.1
3.8.2
4.
Short Term Storage (3 to 7 days) ................................................................................................... 43
Long Term Storage (More than 7 days) ......................................................................................... 43
Example Neutralizer Mode Applications.......................................................................... 44
4.1
System Blank........................................................................................................................... 44
4.1.1
4.1.2
Anion System Blank ..................................................................................................................... 44
Cation System Blank ..................................................................................................................... 44
4.2
Dionex CERS 500e Conditioning ............................................................................................. 45
4.3
System Calibration .................................................................................................................. 45
4.3.1
Analysis of Acid Samples Containing High Concentrations of Transition Metals............................ 45
4.4
Recovery Data for Trace Anions in 25% Sodium Hydroxide .................................................... 46
4.5
Recovery Data for Trace Cations in 24% Sulfuric Acid ............................................................ 46
4.6
Anion Example Applications ................................................................................................... 47
Dionex IonPac® AS11 Gradient ..................................................................................................... 47
Dionex IonPac AS11 Gradient Analysis of 10% Sodium Hydroxide............................................... 48
Dionex IonPac AS12A Isocratic Analysis of 25% Sodium Hydroxide ............................................ 50
Dionex IonPac AS12A Isocratic Analysis of 20% Ammonium Hydroxide...................................... 52
Dionex IonPac AS12A Isocratic Analysis of 25% Tetramethylammonium Hydroxide .................... 53
IonPac AS12A Isocratic Analysis of 25% Tetrabutylammonium Hydroxide ................................... 54
4.6.1
4.6.2
4.6.3
4.6.4
4.6.5
4.6.6
4.7
Cation Example Applications ................................................................................................... 55
4.7.1
4.7.2
4.7.3
4.7.4
4.7.5
5.
Dionex IonPac CS12A Blank and Standard Analysis ..................................................................... 55
Dionex IonPac CS12A Analysis of 24% Sulfuric Acid................................................................... 56
Dionex IonPac CS12A Analysis of 25% Acetic Acid ..................................................................... 57
Dionex IonPac CS12A Analysis of 10% Hydrofluoric Acid ........................................................... 58
Dionex IonPac CS12A Analysis of 22% Phosphoric Acid .............................................................. 59
Troubleshooting Guide ...................................................................................................... 60
5.1
Electrolytically Regenerated Suppressor Operational Status Displays Alarm State.................... 60
5.2
Small or Increasing Analyte Peak Areas ................................................................................... 61
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 6 of 84
Contents
5.2.1
5.2.2
5.2.3
6.
Suppressor Chemical Regeneration Steps ...................................................................................... 61
Suppressor Electrolytic Regeneration Steps ................................................................................... 62
Full or Extended Regeneration: ..................................................................................................... 62
5.3
High Background Conductivity ................................................................................................ 64
5.4
Drifting Baseline ..................................................................................................................... 64
5.5
Noisy Baseline ......................................................................................................................... 64
5.6
Decreased Sensitivity ............................................................................................................... 66
5.7
System Back Pressure Increases Over Time ............................................................................. 66
5.8
Liquid Leaks ............................................................................................................................ 67
5.9
Poor or unstable recovery of certain peaks. .............................................................................. 67
5.10
Peaks and spikes in the absence of an injection ........................................................................ 67
5.11
Low Sample Response (Neutralization Mode Only) ................................................................. 68
5.12
Low Neutralization Capacity (Neutralization Mode Only) ........................................................ 69
Dionex ERS 500, Dionex ERS 500e and
Dionex AERS 500 Carbonate Suppressor Cleanup.......................................................... 70
6.1
Metal Contaminants or Precipitates .......................................................................................... 70
6.2
Organic Contaminants ............................................................................................................. 71
Appendix A – Current Settings ................................................................................................ 72
A.1.
Optimum Current Settings for Common Eluents; Dionex AERS 500 (4 and 2 mm)
and Dionex AERS 500e (4 and 2 mm) ..................................................................................... 72
A.2.
Optimum Current Settings for Common Eluents;
Dionex AERS 500 Carbonate (4 and 2 mm) ............................................................................. 75
A.3.
Optimum Current Settings for Common Eluents; Dionex CERS 500 (4 mm and 2 mm)
and Dionex CERS 500e (4 mm and 2 mm) ............................................................................... 76
A.2
Current Settings for Older Detectors ........................................................................................ 79
Appendix B – Suppressor Controller ....................................................................................... 80
B.1
Dionex ERS 500 and Dionex ERS 500e Control for the CD20/CD25/CD25A
and ED40/ED50/ED50A (Dionex DX-500 instruments) ........................................................... 80
B.1.1
B.1.2
B.1.3
B.1.4
Dionex ERS 500 Control for the Dionex DX-120 .......................................................................... 81
ERS Control for the CDM-3/PED-2 (Dionex DX-300 Instruments) ................................................ 81
ERS Control for the Dionex DX-100 (Model DX 1-03) ................................................................. 81
SRC Controller ............................................................................................................................. 81
Appendix C – Alarm States for Older Instruments ................................................................ 84
C.1
Alarm States for the CD20/ED40 (Dionex DX-500 Instruments) .............................................. 84
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 7 of 84
1 – Introduction
1. Introduction
Suppressor: The role of a suppressor in Ion Chromatography is to remove the eluent and sample
counterions and replace this with regenerant ions thereby converting the eluent to a weakly
dissociated form prior to detection. Detection of analyte ions particularly with conductivity
detection is therefore feasible against a low background. The suppressor not only reduces the
background signal but also the noise associated with the signal. Furthermore, the analytes are
converted to the more conductive acid or base form, which enhances the signal, particularly for
fully dissociated species. Thus overall improvement in detection limits, as observed from the
signal to noise ratio, is achieved. When compared to, applications that do not use a suppressor,
i.e., single column ion chromatography, the improvement in noise with suppressed ion
chromatography far exceeds the noise performance of single column chromatography
applications. Hence the suppressor has become an integral part of the ion chromatography
instrument.
The suppressors from Thermo Fisher Scientific are designed for continuous operation and do
not require any switching or offline regeneration. Furthermore, the standards and the samples
are always exposed to the same suppressor device when pursuing ion analysis, thus ensuring
that the analytical parameters are consistent between calibration and analysis. From a simplistic
perspective there are two types of suppressors offered for continuous operation; electrolytically
regenerated suppressors and chemically regenerated suppressors. The electrolytic suppressors
operate continuously with a water source as a regenerant. In the recycle mode of operation the
water source is derived from the suppressed eluent, thereby making the suppressor operation
facile. The chemical suppressors operate continuously with an external regenerant source.
The electrolytic suppressor device also permits recycle of the eluent when installed in a system
with Eluent Recycle (ER) system.
Additionally, an electrolytic suppressor can also act as an electrolytically regenerated neutralizer
for the neutralization of basic or acidic matrices prior to injection. By neutralizing the matrix
ions, trace anionic or cationic species can be detected in strong acid or base samples.
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 8 of 84
1 – Introduction
1.1 Electrolytically Regenerated Suppressor
The Thermo Scientific™ Dionex™ Electrolytically Regenerated Suppressor (Dionex ERS™
500 Suppressor) replaces the Thermo Scientific Dionex Self-Regenerating Suppressor (Dionex
SRS™ 300 Suppressor) product line. The Dionex ERS 500 is an electrolytic suppressor with a
new hardware design that allows the suppressor to be more pressure tolerant than previous
generation suppressor devices. The suppressor flow pathway has been redesigned to optimize
band dispersion and improve the flow and sealing properties. The Dionex ERS 500 continues to
use the same cleaned ion exchange components (screens and membranes) as the Dionex SRS
300 suppressor devices; however the Dionex ERS 500 eluent channel uses an ion exchange
resin bed as opposed to a gasketed screen.
The Dionex Electrolytically Regenerated Suppressor (Dionex ERS 500) is available in two
versions: the Anion Electrolytically Regenerated Suppressor (Dionex AERS™ 500) and the
Cation Electrolytically Regenerated Suppressor (Dionex CERS™ 500) to support anion and
cation analysis applications. The Dionex ERS 500 system consists of an Electrolytically
Regenerated Suppressor, the Suppressor Control, back pressure coils, and the Gas Separator
Waste Tube, see Figure 1. This high performance, low maintenance AutoSuppression system
provides a reliable solution for Ion Chromatography.
Additionally, the Dionex ERS 500 offers high capacity suppression while adding minimal delay
volume to the analytical system. The Dionex AERS 500 provides continuous suppression of
traditional eluents, and more concentrated eluents up to 200 mM NaOH. The Dionex CERS 500
offers continuous suppression of concentrated eluents up to 100 mN H2SO4 or MSA. This high
capacity significantly expands the capabilities and simplifies the operation of Ion
Chromatography.
The Dionex ERS 500, Dionex ERS 500e and Dionex AERS 500 Carbonate suppressors are
available in 2 mm and 4 mm formats for use with 2, 3, 4, or 5 mm Ion Chromatography columns
and systems. The 2 mm Dionex ERS 500, Dionex ERS 500e and Dionex AERS 500 Carbonate
are specially designed with reduced internal volume to ensure optimum performance with 2 mm
columns and systems.
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 9 of 84
1 – Introduction
Figure 1
The Electrolytically Regenerated Suppressor and Accessories
Dionex ERS 500 Suppressor
1/8 in. o.d. tubing
Gas Separator Tube
Backpressure Coils
Waste Container
Tip
For assistance, contact Technical Support for Dionex Products. In the U.S., call 1-800346-6390. Outside the U.S., call the nearest Thermo Fisher Scientific office.
The Dionex ERS 500 design comprises of three channels defined by two ion exchange
membranes. The central channel is the eluent channel and the two side channels are regenerant
channels. Two PEEK plates form the outer wall of the regenerant channels and have ¼-24 ports
for bringing in the regenerant liquid into and out of the device. The eluent channel is physically
defined by a PEEK plate that seals against the ion exchange membrane and a thin elastomeric
O-ring installed in the regenerant channel. The eluent in and out ports are independent ports that
define the fluidic pathway, similar to a column. The regenerant flow is arranged to be countercurrent to the eluent flow. This orientation ensures complete regeneration of the device
Electrodes are placed along the length of the regenerant channels to completely cover the eluent
channel. In operation, when a DC voltage is applied across the electrodes and the voltage
exceeds the standard potential for the electrolysis of water (approximately 1.5 V), water is
electrolytically split to form electrolysis ions.
At the anode
H2O  2H+ + 1/2O2 + 2e-
At the cathode
H2O + 2e-  2OH- + H2
The electrolysis ions are then available for the suppression reactions. The Dionex ERS 500
suppressor design allows facile transport of cations or anions depending on which type of
suppressor is used for the application. For example, when pursuing anion analysis with a Dionex
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 10 of 84
1 – Introduction
AERS 500, cation exchange functionality extends across the electrodes. The function of this is
to lower the resistance and aid in the transport of ions in and out of the eluent channel. In the
Dionex ERS 500 the eluent channel is filled with ion exchange resin and provides a static
capacity which is particularly useful when eluent is pumped into the device with the power off.
In operation the electrolytically generated hydronium ions in the Dionex AERS 500 are driven
towards the cathode along with eluent cations by the applied voltage. The membrane allows
hydronium ions to pass into the eluent chamber resulting in the conversion of the electrolyte of
the eluent to a weakly ionized form. For each hydronium ion entering the eluent channel one
hydronium or a cation exits the device and is driven towards the cathode. At the cathode the
cations combine with the electrolytically generated hydroxide ions to form water or base.
Overall the current dictates the concentration of hydronium and hydroxide ions.
The eluent suppression process is illustrated for Anion Suppressor in Figure 2 and for Cation
Suppressor in
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 11 of 84
1 – Introduction
Figure 3.
As shown in Figure 2, the water regenerant undergoes electrolysis to form hydroxide ions on the
cathode surface along with hydrogen gas while hydronium ions are formed in the anode surface
along with oxygen gas. In the Anion Suppressor, cation exchange materials such as screens,
membranes and resins allow hydronium ions to move from the anode chamber into the eluent
chamber to neutralize the hydroxide eluent. Sodium ions or eluent or sample counter-ions in the
eluent are driven by the applied electric potential towards the cathode and combine with the
hydroxide ions generated at the cathode to form sodium hydroxide waste. Hydronium ions can
also travel all the way to the cathode to form water, thus effecting suppression of the eluent and
conversion of the analyte to typically a more conductive acid form.
Figure 2
AutoSuppression with the Dionex AERS 500
Dionex AERS 500 in AutoSuppression Mode
The Dionex Electrolytically Regenerated Suppressor for External Water Mode (Dionex ERS
500e) is optimized for use in the external water mode. The Dionex ERS 500e hardware design is
similar to the Dionex ERS 500 hardware design from a sealing perspective and is optimized for
minimal band dispersion and improved flow performance. The Dionex ERS 500e uses the same
ion exchange membranes, screens, and resin materials as the standard Dionex ERS 500
suppressor. The Dionex ERS 500e is configured with a parallel regenerant flow to facilitate easy
supply and removal of regenerant and waste from the regenerant compartments making it
suitable for external water applications, as well as applications where organic modifiers (up to
40%) are present in the eluent or sample. The Dionex ERS 500e is required for applications
where borate is used as an eluent.
The Dionex ERS 500e (2 mm) suppressor is compatible with MS detection; the suppressor has
been designed to have minimal interference for MS applications and is designed for operation in
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 12 of 84
1 – Introduction
external water mode. The 2 mm suppressor is recommended for applications that use MS
detection due to the improved efficiency, and since less eluent enters the MS at the lower
operational flow rate.
The Dionex Anion Electrolytically Regenerated Suppressor for Carbonate Eluents (Dionex
AERS 500 Carbonate) is optimized for use with carbonate or carbonate/bicarbonate based
eluents and is available only as an anion version. The Dionex AERS 500 Carbonate hardware
design is similar to the Dionex AERS 500 hardware design from a sealing perspective and is
optimized for minimal band dispersion and improved flow performance. The Dionex AERS 500
Carbonate uses the same ion exchange membranes, screens, and resin materials as the standard
Dionex AERS 500 suppressor. The Dionex AERS 500 Carbonate is configured with a parallel
regenerant flow to facilitate easy supply and removal of regenerant and waste from the
regenerant compartments. The Dionex AERS 500 Carbonate operates similar to the Dionex
AERS 500 suppressor using electrolysis derived regenerant ions; however, it uses a threeelectrode design for the electrolysis function. The anode electrode is segmented into two
portions with an electrical gap. The anode electrodes are connected via a resistor thus resulting
in a lower applied current across the outlet section of the suppressor relative to the inlet section
of the suppressor. This design results in decreased gas production at the outlet, and as a
consequence, results in less variation in the suppressed background and is able to achieve low
noise.
As shown in
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 13 of 84
1 – Introduction
Figure 3, the water regenerant undergoes electrolysis to form hydroxide ions in the cathode
surface along with hydrogen gas while hydronium ions are formed in the anode surface along
with oxygen gas. In the cation suppressor, anion exchange materials such as screens,
membranes and resins allow hydroxide ions to move from the cathode chamber into the eluent
chamber to neutralize the acid eluent. MSA ions or eluent or sample counter-ions in the eluent
are driven by the applied electric potential towards the anode and combine with the hydronium
ions generated at the anode to form methane sulfonic acid waste. Hydroxide ions can also travel
all the way from the cathode and combine with hydronium ions at the anode to form water, thus
effecting suppression of the eluent and conversion of the analyte to a typically more conductive
base form.
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 14 of 84
1 – Introduction
Figure 3
AutoSuppression with the Dionex CERS 500
Dionex
500
in AutoSuppression
Mode
CSRSCERS
300 in
AutoSuppression
Mode
ANALYTE IN H+ MSA-
ANODE
+
Waste/Vent
ELUENT
(MSA)
MSA + O2
CATHODE
Waste/Vent
-
H2O + H2
H+ + MSAMSA- + H+
OH-
[H+ + OH-]
2H3O+ + ½ O2 (g)
H2O
3H2O
H2 + 2 OH-
2 H2O
ANALYTE IN H2O
H2O
H 2O
TO DETECTOR
1.2 Overview of Suppression and Neutralization Modes
Four basic modes of suppression can be performed with the Dionex Electrolytically
Regenerated Suppressor (Dionex ERS 500, Dionex ERS 500e and Dionex AERS 500
Carbonate):
!

AutoSuppression Recycle Mode



AutoSuppression External Water Mode
MPIC Suppression Mode
Neutralization Mode
The Dionex ERS 500 is not compatible with the Chemical Suppression Mode. The Dionex
CRS 500 or equivalent should be used when Chemical Suppression Mode is required.
NOTE
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 15 of 84
1 – Introduction
1.2.1
Mode of Operation Selection
The Dionex ERS 500 mode of operation depends mainly on the eluent composition, the analysis
sensitivity requirements and the sample matrix. The compatibilities are shown in
Table 1. For example, eluents containing organic solvents that tend to oxidize easily are not
compatible with the AutoSuppression Recycle Mode. The AutoSuppression External Water
Mode should be used instead, or a Dionex CRS suppressor employed in Chemical Suppression
Mode. The MPIC Suppression Mode is specifically designed for applications where ion-pair
reagents and solvents are present in the eluent. The Neutralization Mode can be used with any
eluent composition.
Table 1
Eluent Composition and Suppression Mode Compatibility
Eluent
Composition
Aqueous Eluents (excluding
borate)
Borate Eluents
Eluents containing
Organic Solvents that are
easily oxidized
Eluents containing Organic
Solvents that are not easily
oxidized
Eluents Containing Ion Pair
Reagents with or without
Solvents
Simple Aqueous Samples
Suppression
Recycle
Yes
Suppression
External Water(1)
Yes
Chemical
Suppression(2)
Yes
MPIC
Suppression
N/A
No
No
Yes(3)
Yes (Up to 40%)(3)
Yes
Yes (Up to 100%)
N/A
N/A
Yes
Yes
Yes
Yes
No
No
No
Yes
Yes
Yes
Yes
Complex Samples or
Samples containing Solvents
No
Yes(3)
Yes
Yes (assuming Ion
Pairing Reagent)
Yes (assuming Ion
Pairing Reagent)
(1)
The Dionex ERS 500 is not recommended for External Water Mode; the Dionex ERS 500e is recommended for External Water Mode
applications.
(2)
The Dionex ERS 500 does not support Chemical Suppression Mode. Use of a Chemically Regenerated Suppressor such as the CRS 500
or equivalent is recommended.
(3)
Only the Dionex ERS 500e is compatible with Borate Eluents and Eluents or Samples containing Organic Solvents that are easily
oxidized.
1.2.2
The AutoSuppression Recycle Mode
The AutoSuppression Recycle Mode uses the suppressed conductivity cell effluent as the source
of water for the regenerant. This is the preferred method of operation for the Dionex ERS 500
and Dionex AERS 500 Carbonate. The Dionex ERS 500e is also compatible with this mode.
The advantage of this mode of operation is simplicity and ease of use. This mode reliably
provides AutoSuppression for most suppressed conductivity applications using solvent-free
eluents. For solvents that are not easily oxidized, such as iso-propyl alcohol, the
AutoSuppression recycle mode is preferred. As the eluent passes through the suppressor’s
eluent channel it is converted to a weakly ionized form. After detection, the cell effluent can be
routed back to the regenerant channel to provide the water required for the electrolysis reactions.
The amount of water flowing through the regenerant chambers is therefore limited to the eluent
flow rate. See Section 3 for complete operating instructions.
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 16 of 84
1 – Introduction
!
The AutoSuppression Recycle Mode is not compatible with eluents containing Borate or
Organic Solvents that tend to oxidize easily, such as methanol.
NOTE
1.2.3
The AutoSuppression External Water Mode
The AutoSuppression External Water Mode is used for any application requiring organic
solvents in the eluent or sample, or for applications using borate as the eluent ion. This is the
preferred method of operation for the Dionex ERS 500e. The Dionex ERS 500 and Dionex
AERS 500 Carbonate are compatible with this mode, but not with applications requiring
organic solvents in the eluent, or for applications using borate as the eluent ion. This mode
uses a constant source of deionized water from a pressurized bottle or other source of deionized
water that delivers 0.25 to 2 mL/min for 2 mm applications and 1.0 to 5 mL/min for 4 mm
applications, although the eluent flow rate is typically recommended as double the regenerant
flow rate. The amount of water flowing through the regenerant chambers is independent of the
eluent flow rate. The AutoSuppression External Water Mode eliminates the potential for buildup of contaminating ions resulting from the oxidation of solvents. It is also recommended when
pursuing analysis with high concentrations of matrix ions. Any analysis performed using the
AutoSuppression Recycle Mode can also be performed using the AutoSuppression External
Water Mode. See Section 3 for complete operating instructions.
1.2.4
The Chemical Suppression Mode
The Dionex ERS 500 cannot be used in the Chemical Suppression Mode. The peak response
will not be maintained to a constant level in this mode. Thermo Scientific Dionex recommends
the use of a Chemically Regenerated Suppressor (Dionex CRS 500 or equivalent) for chemical
suppression applications. Please refer to the Dionex CRS Manual, Document No. 031727, and
the DCR Kit Manual, Document No. 031664.
The Dionex AERS 500 Carbonate and Dionex ERS 500e can be used in the Chemical
Suppression Mode; however the total suppression capacity is reduced compared to a
Chemically Regenerated Suppressor. Thermo Scientific recommends the use of a Chemically
Regenerated Suppressor such as the Dionex CRS 500 for chemical suppression applications.
1.2.5
The MPIC Suppression Mode
1.2.5.1
Anion MPIC
The Dionex AERS 500e is used for eluent suppression of Mobile Phase Ion Chromatography
(MPIC or ion-pairing) eluents by using the MPIC Suppression Mode. The Dionex AERS 500
and Dionex AERS 500 Carbonate are not recommended for this mode. The MPIC
Suppression Mode is a combination of the AutoSuppression External Water Mode augmented
with a chemical regenerant such as sulfuric acid (H2SO4). The MPIC Suppression Mode uses an
applied current and a constant source of dilute sulfuric acid solution from a pressurized bottle
delivery system. This mode must be used for MPIC applications requiring an ion pair reagent
and organic solvents in the eluent. The MPIC Suppression Mode reliably provides suppression
of typical eluents for MPIC applications using suppressed conductivity detection. The ion pair
reagents, such as tetrabutylammonium hydroxide (TBAOH), are used in concentrations
typically ranging from 1.0 to 5.0 mM. See Section 3 for complete operating instructions.
1.2.5.2
Cation MPIC
The Dionex CERS 500e is used for eluent suppression of MPIC eluents by using the
AutoSuppression External Water Mode or the MPIC Suppression Mode depending on the
specific MPIC application. The Dionex CERS 500 is not recommended for this mode. The
MPIC Suppression Mode uses an applied current and a constant source of dilute boric acid
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 17 of 84
1 – Introduction
regenerant solution from a pressurized bottle delivery system. Dilute boric acid is added to the
water regenerant to enhance detection and improve linearity of weak bases such as ammonia
and amines. This mode is used for MPIC applications requiring an ion pair reagent and organic
solvents in the eluent. The MPIC Suppression Mode reliably provides suppression of typical
eluents for MPIC applications using suppressed conductivity detection. The ion pair reagents,
such as octanesulfonic acid (OSA), are used in concentrations typically ranging from 1.0 to 5.0
mM. Organic solvent concentrations should not exceed 40%. See Section 3 for complete
operating instructions.
1.2.6
The Neutralization Mode
The Dionex ERS 500 (4 mm) when operated in this mode requires a constant deionized water
flow of 3 to 5 mL/min through the regenerant chambers. The regenerant water can be delivered
from a pressurized bottle or pump. If using a pressurized bottle, it is recommend to use the
Dionex ERS 500e.
IMPORTANT
1.2.6.1
The Dionex AERS 500 Carbonate, Dionex ERS 500 (2 mm) and Dionex ERS 500e (2 mm)
suppressors are not designed or validated for the Neutralization Mode. The Dionex ERS
500e (4mm) suppressor is recommended and validated for this mode. The Dionex ERS 500
(4 mm) suppressor may also be used for this mode.
Neutralization Theory
Figure 4
AutoNeutralization of Concentrated Acid/Base with the Self-Regenerating
Neutralizer
Dionex AERS 500
Dionex CERS 500
As shown in Figure 4, when a potential is applied across the two electrodes, the water
regenerant undergoes electrolysis, forming hydronium ions at the anode and hydroxide ions at
the cathode.
In the Dionex AERS 500, hydronium is transported from the anode towards the cathode and
enters the sample chamber. At the same time, the matrix cations in the sample move towards the
cathode and enter the cathode chamber. The matrix cations combine with the hydroxide ions at
the cathode forming a base, and are removed from the regenerant chamber. The net effect of this
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 18 of 84
1 – Introduction
transport of ions is neutralization of the sample matrix ions to a weakly ionized form while the
sample anions are typically converted to a strongly ionized form. The sample anions are now in
a weakly dissociated matrix such as water and are ready for analysis.
In the Dionex CERS 500 hydroxide is transported from the cathode towards the anion and enters
the sample chamber. At the same time, the matrix anions in the sample move towards the anode
and enter the anode chamber. The matrix anions combine with the hydronium ions at the anode
forming an acid and are removed from the regenerant chamber. The net effect of this transport
of ions is neutralization of the sample matrix ions to a weakly ionized form, while the sample
cations are typically converted to a strongly ionized form. The sample cations are now in a
weakly dissociated matrix such as water and are ready for analysis
1.2.6.2
Trace Ion Analysis After Neutralization
After neutralization, the sample can be fed into any Dionex Ion Chromatography system and
operated in the concentrator mode. It is important that only low pressure concentrator columns,
such as the Dionex UTAC-XLP2 (Item # 072781) and Dionex TCC-XLP1 (Item # 063889), be
used to protect the Dionex ERS 500 from damage.
1.3 Shipment and Storage
1.3.1
Shipment
!
CAUTION
1.3.2
The Electrolytically Regenerated Suppressors (Dionex ERS 500, Dionex ERS 500e, and
Dionex AERS 500 Carbonate) should not be subjected to temperatures above 50°C for long
durations during shipment, storage or operation, or for any duration above 80°C.
Storage
!
CAUTION
Thermo Scientific
031956-11
Ensure the suppressor is stored in a temperature controlled environment away from direct
exposure to sunlight or other sources of heat. Do not store the suppressor in an
environment where temperatures in excess of 50°C may be experienced, such as a parked
car.
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 19 of 84
2 – Installation
2. Installation
2.1 System Requirements
The Dionex ERS 500 and Dionex ERS 500e are designed to be direct replacements for the
Dionex SRS series of suppressors, such as the Dionex SRS I, Dionex SRS II, Dionex SRS
ULTRA, Dionex SRS ULTRA II and Dionex SRS 300. The Dionex ERS 500 can be used in
place of any of these suppressors where recycled eluent mode is employed.
The Dionex ERS 500e is designed to be a direct replacement for the Dionex SRS series of
suppressors where external water mode is employed. The Dionex ERS 500e features a hardware
design that significantly improves regenerant flow when operated in external water mode.
The Dionex AERS 500 Carbonate suppressor is designed for operation with carbonate and
carbonate/bicarbonate eluents. The Dionex AERS 500 Carbonate features a hardware design
that significantly improves noise performance with carbonate eluents without sacrificing
performance or ease-of-use. The unique hardware design delivers the lowest noise level of any
electrolytic suppressor for carbonate eluents.
The Dionex ERS 500 and Dionex ERS 500e are not designed to be direct replacements for the
Dionex SRS series of suppressors where these suppressors are being used in Chemical
Suppression Mode. If Chemical Suppression Mode is being used, the Chemically Regenerated
Suppressor (Dionex CRS 500) or equivalent is recommended. The Dionex AERS 500 Carbonate
and Dionex ERS 500e can be used in the Chemical Suppression Mode; however the total
suppression capacity is reduced compared to a Chemically Regenerated Suppressor.
The Dionex ERS 500e is also designed to be a direct replacement for the Dionex SRN series of
neutralizers, such as the Dionex SRN 300, Dionex SRN-II and Dionex SRN products.
The Dionex ERS 500, Dionex ERS 500e and Dionex AERS 500 Carbonate are designed to be
run on any Dionex Ion Chromatography System (ICS) equipped with an analytical Anion or
Cation exchange column set and an electrolytic suppressor controller, such as the Thermo
Scientific Dionex ICS-5000+, Dionex Integrion, or Dionex Aquion. They are not designed to be
run on a Dionex Capillary Ion Chromatography Systems, such as the ICS-4000, or on Dionex
Ion Chromatography Systems that do not have an electrolytic suppressor controller, such as the
Dionex ICS-90A, Dionex ICS-600 or Dionex ICS-900. Some legacy systems require a
standalone controller for installation of the Dionex ERS 500, Dionex ERS 500e or Dionex
AERS 500 Carbonate. See, “Electrolytically Regenerated Suppressor Requirements for
Selected IC Modules.”
The Dionex ERS control is provided by discrete (50, 100, 300, and 500mA) or non-discrete (0
to 500 mA in 1 mA increment) power supplies. Discrete Dionex ERS Control is integrated into
older systems, such as the CDM-3 and PED-2 of the Dionex DX-300, the Dionex DX-100
(Model 1-03), Dionex DX-120, Dionex DX-320 (IC20 and IC25 models), Dionex DX-500
(CD20 and ED40 detectors), and Dionex DX-600 (CD25 and ED50 detectors). The use of
discrete power supplies may not be suitable for optimal suppressor performance and may
affect the suppressor noise performance and life time.
It is therefore recommended to use a non-discrete power supply, such as the Thermo Scientific
Dionex RFC-10 or Dionex RFC-30. It is also possible to use the discrete power supplies with a
Dionex SCC-10 controller that provides twelve discrete current settings. Non-discrete Dionex
ERS Control is integrated into modern instruments, including the Dionex ICS series (excluding
Dionex ICS-90, Dionex ICS-90A, Dionex ICS-600 and Dionex ICS-900), Dionex Integrion and
Dionex Aquion.
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 20 of 84
2 – Installation
The older Dionex SRC-1 controller has been discontinued. The replacement product is either
the Dionex RFC-10 controller or the Dionex RFC-30 controller. The Dionex RFC-10 controller
provides 1 mA graduated control of an electrolytic suppressor. The Dionex RFC-30 controller
provides 1 mA graduated control of an electrolytic suppressor in addition to providing control of
an Eluent Generator Cartridge (Dionex EGC) and a Continuously Regenerated Trap Column
(Dionex CR-TC). The Dionex SCC-10 suppressor current controller can be used in conjunction
with the Dionex SRC-1 controller to provide a current output of twelve settings.
For optimal suppressor performance it is important to operate the suppressor at the
recommended current settings as recommended by Chromeleon or as calculated from Section
2.6.1. It is highly recommended to set the current setting for the Dionex ERS 500 or Dionex
AERS 500 Carbonate to the exact calculated current (within 1 mA). Older power supplies do
not have the 1 mA graduated control. Failure to set the current accurately could reduce
suppressor performance and life-time
Table 2
Self-Regenerating Suppressor Requirements for Selected Ion
Chromatographs
Dionex Ion Chr omatograph
Series Module
2000i
QIC
2000 SP
4000i
4500i
8000
8100
8200
DX-100
Model DX 1-03
DX-120
DX-300
CDM-2, PED
CDM-3, PED-2
DX-320 with IC20 or IC25
DX-320 with IC25A
DX-500
DX-600 with CD25A or ED50A
DX-600 with CD25 or ED50
DX-800
Thermo Scientific
031956-11
Dionex RFC-10, Dionex RFC-30 or
Dionex SCC-10
Contr oller Requirement
YES
YES
YES
YES
YES
YES
N/A
N/A
YES
RECOMMENDED
RECOMMENDED
N/A
YES
RECOMMENDED
RECOMMENDED
NO
RECOMMENDED
NO
RECOMMENDED
RECOMMENDED
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 21 of 84
2 – Installation
The Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500 Carbonate is installed in the
column compartment or detector compartment of the chromatography module immediately after
the analytical column and before the conductivity detector cell. All components required for
installation of the suppressor are included with the system.


Gas Separator Waste Tube (Item # 045460)
Backpressure coil(s)
o 4 mm (Item # 045877)
o 2 mm (Item # 045878)


Microbore Tubing (2 mm only) (Item # 052324)
Mounting clip (Item # 045612)
Options:
!
CAUTION
!

Dionex CRS/ERS Installation Kit (Item # 038018)
(Pressurized Water Delivery System used with AutoSuppression External Water Mode,
Chemical Suppression Mode, or MPIC Suppression Mode).

Peristaltic Pump Kit (Item #: 064508). (Water delivery system used with
AutoSuppression External Water Mode, Chemical Suppression Mode, or MPIC Mode).
The Dionex ERS 500, Dionex ERS 500e and Dionex AERS 500 Carbonate must be
operated with the Gas Separator Waste Tube (Item # 045460)
The use of ¼-28 or 10-32 ferrule/bolt style liquid lines may be required for installation and
use of ERS 500. See “Dionex Liquid Line Fittings” for complete details.
NOTE
2.2 Electrolytically Regenerated Suppressor Control
!
CAUTION
!
CAUTION
!
NOTE
Thermo Scientific
031956-11
Always turn the pump and the Dionex ERS Control on and off at the same time. Eluent
flow through the Dionex ERS 500 is required for proper operation. Without current, the
membranes and screens in the Dionex ERS 500 will become exhausted by the flowing
eluent resulting in small analyte peak areas. If this should occur, perform the procedure in
Section 5.2.
The Dionex AERS 500 Carbonate suppressor is not compatible with discrete power
supplies and should only be operated with power supplies that can deliver current in 1 mA
increments.
If an ERS Mode is not available, select the equivalent SRS Mode on the power supply to
support the Dionex ERS 500 suppressor. The Dionex ERS 500 and Dionex ERS 500e are
fully compatible with SRS settings.
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 22 of 84
2 – Installation
!
NOTE
2.2.1
The Dionex AERS 500 Carbonate suppressor has different electrical current requirements
than the Dionex AERS 500 suppressor. If an AERS Carbonate mode is not available the
current applied to a Dionex AERS 500 Carbonate should be adjusted by a factor of 1.30
compared to a standard AERS 500 suppressor. Chromeleon 7.2 SR3 MUa and later
versions have an option for selecting an AERS_Carbonate suppressor in the Instrument
Method Wizard and Editor dialog box. Selecting this suppressor from the drop-down list
will automatically apply the recommended current setting for Dionex AERS 500 Carbonate
suppressors.
Dionex Reagent-Free Controller (Dionex RFC)
The Dionex Reagent-Free Controller (Dionex RFC) is an external power supply available in two
versions.


!
NOTE
!
NOTE
The Dionex RFC-10 controls the Dionex ERS 500 Electrolytically Regenerated
Suppressor. Current is delivered at 1 mA resolution.
The Dionex RFC-30 controls an Dionex ERS 500 Electrolytically Regenerated
Suppressor, as well as a Dionex Eluent Generator Cartridge (Dionex EGC) and a
Dionex CR-TC Continuously Regenerated Trap Column. Current is delivered at 1 mA
resolution.
If an ERS Mode is not available, select the equivalent SRS Mode on the power supply to
support the Dionex ERS 500 suppressor. The Dionex ERS 500 and Dionex ERS 500e are
fully compatible with SRS settings.
The Dionex AERS 500 Carbonate suppressor has different electrical current requirements
than the Dionex AERS 500 suppressor. If an AERS Carbonate mode is not available the
current applied to a Dionex AERS 500 Carbonate should be adjusted by a factor of 1.30
compared to a standard AERS 500 suppressor.
The Dionex RFC controls these devices by supplying current to the suppressor and for the
Dionex RFC-30, current to the eluent generator and voltage to the Dionex CR-TC. Please see
the Dionex RFC Operator’s Manual for suppressor operating and installation instructions with
the following Dionex products:

Dionex DX-320/320J

Dionex DX-500, Dionex DX-600, or Dionex ICS-2500

Dionex DX-120
2.3 Dionex SCC-10 Suppressor current controller (Item # 074053)
The Dionex SCC-10 is an external controller designed for use with legacy instruments that only
offer four settings for suppressor current, and is recommended for optimal performance. The
Dionex SCC-10 is powered from the existing suppressor current supply, and can output twelve
discrete current settings from 10 mA to 250 mA.
!
The Dionex SCC-10 external controller is recommended when using legacy instruments
that only offer four settings for suppressor current to achieve optimum performance.
NOTE
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 23 of 84
2 – Installation
2.4
Back Pressure Coils for the Dionex ERS 500, Dionex ERS 500e and Dionex
AERS 500 Carbonate
All detector cells require enough back pressure to prevent eluent in the cell from out-gassing
due to abrupt volume changes between the small inner diameter of the connecting tube and the
relatively larger volume of the cell; out-gassing creates bubbles in the cell and disrupts detector
responsiveness. For example, carbonate eluent is suppressed to carbonic acid, which is CO2 gas
in equilibrium with DI water, and CO2 gas can come out of solution if adequate pressure is not
applied. The above out-gassing can trap bubbles in the cell causing high noise. Therefore,
Thermo Scientific Dionex recommends the addition of 30-40 psi of backpressure after the cell.
It should be noted that for RFIC hydroxide or MSA applications it may be possible to operate
the cell without backpressure. However, for carbonate and/or bicarbonate applications it is
highly recommended to install backpressure coils.
Back pressure coil components are shipped with your system. For 4 mm systems, locate
assembly Item # 045877. For 2 mm systems, locate assembly Item # 045878 (for 2 mm
systems, the backpressure coils are available in the microbore tubing kit, Item # 052324).
Alternatively, lengths and diameters of tubing necessary for proper back pressure are given in
Table 3, “Coils for ERS 500 Back Pressure Requirements.” Adjust the tubing length to achieve
a backpressure of approximately 40 psi.
!
CAUTION
2.4.1
If back pressure coils become damaged or plugged, they may cause irreversible damage to
the suppressor.
Assembly
A. Slip PEEK liquid line bolts and ferrules onto the ends of the tubing. Refer to Table 3,
“Coils for ERS 500 Back Pressure Requirements,” and determine the correct number of
coils required for your application based on the eluent flow rate.
B. After assembly of the coils, see Figure 5 The Auto Suppression Recycle Mode
Plumbing Diagram” for the proper placement of the completed coils and couplers
between the suppressor and the Gas Separator Waste Tube.
Table 3
ERS 500
Type
4 mm
4 mm
2 mm
2 mm
!
CAUTION
Thermo Scientific
031956-11
Coils for Dionex ERS 500, Dionex ERS 500e and Dionex AERS 500 Carbonate
Back Pressure Requirements
Item #
Flow Rate
045877
045877
045878
045878
0.5–1.5 mL/min
1.5–3.0 mL/min
0.12–0.25 mL/min
0.25–0.75 mL/min
I.D. of
Tubing
0.010” (Black)
0.010” (Black)
0.005” (Red)
0.005” (Red)
Length of
Each Coil
2.5 ft.
2.5 ft.
1.0 ft.
1.0 ft.
Number
of Coils
2
1
2
1
The correct amount of back pressure for optimum operation is 40 psi. Back pressure over
450 psi after the Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500 Carbonate can
cause irreversible damage.
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 24 of 84
2 – Installation
2.5 Gas Separator Waste Tube for the Dionex ERS 500
The Gas Separator Waste Tube (Item # 045460) is an integral part of the Dionex ERS 500
system. It separates any electrolytic gases generated in the Dionex ERS 500, Dionex ERS 500e
or Dionex AERS 500 Carbonate during electrolysis. The Gas Separator Waste Tube is used to
avoid concentrating the gases (particularly hydrogen gas) in the waste container. The Gas
Separator Waste Tube is shipped in one of the Ship Kits of your system.
!
Do not cap the waste reservoir.
CAUTION
!
SAFETY
2.5.1
Minimal hydrogen gas generated by the Dionex ERS 500, Dionex ERS 500e or Dionex
AERS 500 Carbonate is not dangerous unless the gas is trapped in a closed container and
allowed to concentrate. The Gas Separator Waste Tube must be open to the atmosphere
and not in a confined space to operate properly.
Assembly
A. Assemble and install the Gas Separator Waste Tube and waste line following the steps
below. See Figure 5 The Auto Suppression Recycle Mode Plumbing Diagram”.
B. Use one or two couplers (Item # 045463) to connect two or three lengths of ½” i.d.
black polyethylene tubing (Item # 045462) depending on the waste container depth.
Extend the top of the Waste Separator Tube above the top of the Waste container.
C. Place the Gas Separator Waste Tube with the 1/8” o.d. tubing attached into the waste
container. Ensure the bottom of the Gas Separator Waste Tube is resting on the floor of
the waste container, the top of the device (where the white 1/8” o.d. tubing meets the
black ½” o.d. tubing) is above the top of the container, and that both the Gas Separator
Waste Tube and the waste container are open to the atmosphere.
2.6 Electrolytically Regenerated Suppressor Current Selection
Lower current is better for the performance of the Dionex ERS 500, Dionex ERS 500e and
Dionex AERS 500 Carbonate suppressors. Excess current through the suppressor devices causes
excess heat generation and over time will cause the ion exchange materials to degrade, thus
shortening suppressor lifetime. Excess current can also cause poor recoveries of certain
analytes, particularly magnesium, manganese, and phosphate. No more than 10% above the
optimum current setting is recommended for extended periods of time.
Cooling the suppressor would provide improved noise and lifetime performance. A temperature
setting of 20˚C for the thermal compartment, such as the DC, is recommended.
2.6.1
Calculating the Optimum Current Setting
The optimum current setting depends on the eluent concentration, sample counterion
concentration, flow rate, and mode of operation. If the sample counterion concentration exceeds
the eluent concentration, use the sample counterion concentration in the calculation discussed
below.
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 25 of 84
2 – Installation
These calculations are specific for the type of suppressor. These settings are also applicable in
the presence of standard solvents such as methanol or isopropyl alcohol for anion applications
and acetonitrile for cation applications.
When operated in the Neutralization Mode, the Dionex ERS 500e current should always be set
to 500 mA.
Current (mA) = [flow rate (mL/min)] × [el uent concentration (mN)] ×
[suppressor specific factor]
The factors are listed in the table below. The unit for eluent concentration is mN (not mM).
Table 4
Optimum Suppressor Settings
Suppressor Type
Dionex AERS 500 and AERS 500e
Dionex CERS 500 and CERS 500e
Dionex Atlas (Anion and Cation)
Dionex AERS 500 Carbonate
!
Suppressor Specific Factor
2.47
2.94
3.22
Always round the calculated optimum current up to the nearest whole integer.
NOTE
All modern Thermo Scientific Dionex detectors and suppressor power supplies can be used to
set the current at the calculated value with a minimum current resolution of 1 mA. A Dionex
RFC-10, Dionex RFC-30, or Dionex SCC-10 Controller is recommended for older systems
that only set the current in discrete values of 50, 100, 300 or 500 mA.
!
NOTE
!
CAUTION
!
NOTE
A lower flow rate requires a lower current. A 2 mm Dionex ERS 500 or Dionex ERS 500e
should NEVER be operated at a current above 100 mA. A 2 mm Dionex AERS 500
Carbonate should NEVER be operated at a current above 30 mA.
The Dionex AERS 500 Carbonate suppressor is not compatible with discrete power
supplies and should only be operated with power supplies that can deliver current in 1 mA
increments.
Chromeleon 7.2 SR3 MUa and later have an option for selecting an AERS_Carbonate
suppressor in the Instrument Method Wizard and Editor dialog box. Selecting this
suppressor from the drop-down list will automatically apply the recommended current
setting for the Dionex AERS 500 Carbonate suppressors. Earlier versions of Chromeleon
do not include the correct Suppressor Specific Factor; therefore, the correct current must
be calculated and entered manually if the Dionex AERS 500 Carbonate suppressor is used.
A. Maximum Suppression Capacity
The Maximum Suppression Capacity (MSC) depends on the eluent concentration and flow
rate. The MSC can be calculated using the following equation.
MSC (mN * mL/min) = flow rate (mL/min) * sum of eluent concentration (mN)
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 26 of 84
2 – Installation
Table 5
Maximum Suppression Capacity for Dionex ERS 500 and Dionex ERS 500e
Suppressor
Flow Rate (mL/min)
Maximum Suppression Capacity
Dionex AERS 500 4 mm
0.5 - 3.0
≤ 200 µ equivalents
Dionex AERS 500 2 mm
0.25 - 0.75
< 50 µ equivalents
Dionex AERS 500 2 mm
0.10 - 0.25
< 30 µ equivalents
Dionex CERS 500 4 mm
0.5 - 3.0
< 100 µ equivalents
Dionex CERS 500 2 mm
0.25 - 0.75
< 35 µ equivalents
Dionex CERS 500 2 mm
0.10 - 0.25
≤ 20 µ equivalents
Dionex AERS 500e: Same as Dionex AERS 500
Dionex CERS 500e: Same as Dionex CERS 500
Dionex AERS 500 Carbonate 4 mm : 0.5 – 3.0 : ≤ 30 µ equivalents
Dionex AERS 500 Carbonate 2 mm : 0.25 – 1.0 : ≤ 7.5 µ equivalents
B. Sum of Eluent Concentration Calculation
When using a Dionex AERS 500, Dionex AERS 500e or Dionex AERS 500 Carbonate, the
sum of the eluent concentration can be calculated from the equations below.
Dionex AERS 500 (4 mm) or Dionex AERS 500 Carbonate (4 mm)
Sum of eluent concentration (mN) = {2* Carbonate (mM) + Bicarbonate (mM) + hydroxide
(mM) + 2 * Tetraborate (mM) + custom eluent cation (mN)}
where Tetraborate is <= 50 mM
Dionex AERS 500 (2 mm) or Dionex AERS 500 Carbonate (2 mm)
Sum of eluent concentration (mN) = {2 * Carbonate (mM) + Bicarbonate (mM) + hydroxide
(mM) + 2 * Tetraborate (mM) + custom eluent cation (mN)}
where Tetraborate is <= 75 mM
Custom eluent cation (mN) can be calculated from the normality of the eluent concentration.
!
NOTE
Normality is Equivalents/L of solution. For example, 20.0 mM sodium acetate
(CH3COONa) has 20.0 mN sodium as the cation and 20.0 mM sodium sulfate (Na2SO4) has
40.0 mN sodium as the cation.
When using the Dionex CERS 500 or Dionex CERS 500e, the sum of the eluent concentration
can be calculated using the equations below.
Dionex CERS 500
Sum of eluent concentration (mN) ={2 * Sulfuric Acid (mM) + MSA (mM) + custom eluent
anion (mN)}
Custom eluent anion (mN) can be calculated from the normality of the eluent concentration.
!
Normality is Equivalents/L of solution. For example, 20.0 mM sodium sulfate (Na2SO4)
has 40.0 mN sulfate as the anion.
NOTE
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031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 27 of 84
3 – Operation
3. Operation
This section provides instructions for the start-up and operation of the ERS series suppressors,
including the selection process and suppression modes of operation.
3.1 Chemical Purity Requirements
Precise and accurate results require eluents free of ionic impurities; chemicals and deionized
water used to prepare eluents must be pure as described below. Low trace impurities and low
particulate levels in eluents and regenerants also help protect the Dionex ERS 500 and system
components from contamination. Dionex ERS 500 performance is not guaranteed when the
quality of the chemicals and water used to prepare eluents has been compromised.
3.1.1
Inorganic Chemicals
Reagent Grade inorganic chemicals should always be used to prepare ionic eluents. Preferably,
a lot analysis on each label will certify each chemical as meeting or surpassing the latest
American Chemical Society standard for purity, a universally accepted standard for reagents.
3.1.2
Solvents
Since solvents used with the Dionex ERS 500e are added to ionic eluents to modify the ion
exchange process or improve sample solubility, the solvents used must be free of ionic
impurities. However, since most solvent manufacturers do not test for ionic impurities, the
highest grade of solvents available should be used. Currently, several manufacturers are making
ultra-high purity solvents that are compatible for HPLC and spectrophotometric applications.
These ultra-high purity solvents will usually ensure that your chromatography is not affected by
ionic impurities in the solvent. Dionex has obtained consistent results using High Purity
Solvents manufactured by Burdick and Jackson and Optima ® Solvents by Thermo Fisher
Scientific.
3.1.3
Deionized Water
The deionized water used to prepare eluents should be degassed Type I Reagent Grade Water
with a specific resistance of 18.2 megohm-cm. The water used for the AutoSuppression
External Water Mode should have a specific resistance of 18.2 megohm-cm or greater. The
deionized water should be free of ionized impurities, organics, microorganisms and particulate
matter larger than 0.2 µm. It is good practice to filter eluents through a 0.2 µm filter whenever
possible. Bottled HPLC-Grade Water should not be used since most bottled water contains an
unacceptable level of ionic impurities. Finally, thoroughly degas all deionized water prior to
preparing any eluents or regenerants.
3.2 Start-up
The Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500 Carbonate is installed in the
column or detector chamber of the chromatography module right after the analytical column and
before the conductivity detector cell. On the Dionex ICS-5000+, the suppressor mounts on the
conductivity detector module in the DC. On the Dionex Integrion, the suppressor mounts on the
conductivity detector module in the detector compartment. On the Dionex Aquion, the
suppressor mounts on the component panel behind the front door. Refer to the Dionex IC
System Operator’s Manual for further details.
Orient the suppressor with the ELUENT IN port and the cable at the top if installed vertically;
align the slots on the back of the Dionex ERS 500 with the tabs on the panel. Press in, and then
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 28 of 84
3 – Operation
down to lock the Dionex ERS 500 in place. Lift up and pull out to remove the suppressor.
Ensure the suppressor is plumbed properly according to the selected mode of operation. Refer to
Section 2, “Installation,” for complete installation instructions.
!
WARNING
!
CAUTION
!
Keep the regenerant chambers full with the appropriate regenerant solution or water. The
membranes and screens in the Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500
Carbonate must be completely hydrated to maintain liquid seals and chromatographic
performance.
The correct amount of back pressure on the conductivity detector for optimum operation is
40 psi. Connect the back pressure coil(s) appropriate for your column i.d. and flow rate.
Back pressures over 450 psi after the Dionex ERS 500, Dionex ERS 500e or Dionex AERS
500 Carbonate can cause irreversible damage.
Do not cap the waste reservoir.
CAUTION
!
SAFETY
3.2.1
Hydrogen gas generated by the Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500
Carbonate is not dangerous unless the gas is trapped in a closed container and allowed to
accumulate. The Gas Separator Waste Tube must be open to the atmosphere, and not in a
confined space, to operate properly.
Hydration
Hydrating the suppressor ensures that the ion exchange membranes are in a swollen form for
proper operation. A 20 minute static step is recommended during first time installation to ensure
complete hydration.
1.
If also installing a new guard and/or analytical column, follow the column startup
procedure(s) before moving on to Step 2. The column waste should be diverted to
waste for at least 10 column volumes before installing the new suppressor.
2.
Install the suppressor in the system and plumb the unit in the recycle mode (refer to
Figure 5, below).
3.
Pump ≤10 mM eluent at the application flow rate into the suppressor for 5 minutes,
from the Eluent IN port. The power to the suppressor must be off during this step.
Care should be taken not to exceed 100 psi of backpressure on the ERS suppressor.
The backpressure to the suppressor includes any tubing, cell and backpressure coils
that are connected to the eluent out port of the suppressor.
Thermo Scientific
031956-11
4.
Turn off the pump and allow the suppressor to sit for approximately 20 minutes to fully
hydrate the suppressor resin, screens, and membranes.
5.
After completion of Steps 1 through 4, normal operation may resume using the
application eluent strength and current recommendation.
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 29 of 84
3 – Operation
!
Care should be taken with this procedure not to exceed 100 psi of backpressure on the
suppressor.
NOTE
A short piece of waste tubing should be installed in the Eluent In port during this
procedure to ensure that liquid exiting the suppressor does not well up and flow back into
the cover. This can give a false impression of an internal leak.
!
NOTE
3.2.2
Back Pressure Coil Pressure Test
Injection
Valve
1.
Disconnect the eluent line from the injection valve to the column at the
column inlet.
2.
Connect the eluent line from the injection valve directly to the detector cell
inlet with the recommended number of back pressure coils attached for your
application (see the table below). Turn the pump on at your application flow
rate. After 2 to 3 minutes of equilibration record pressure P1.
3.
Disconnect the back pressure coils and with the pump on measure the system
pressure P2.
Cell
Coil
#2
Coil
#1
4 mm Chromatography
W aste
2 mm Chromatography_______________
1.0 mL/min. = 2 black backpressure coils
(Item # 045877)
(Item # 045878)
2.0 mL/min. = 1 black backpressure coil
(Item # 045877)
Injection
Valve
4.
0.25 mL/min. = 2 red backpressure coils
0.50 mL/min. = 1 red backpressure coil
(Item # 045878)
The correct operating pressure range for the backpressure coil being tested is
Dionex ERS 500 P1 – P2 = 30–40 psi
If the pressure is greater than 40 psi, then trim the back pressure coil and
repeat step 2 and 3 to achieve 30-40 psi in step 4.
Cell
If it is less than 30 psi, then add more tubing to achieve 30 – 40 psi.
W as te
3.2.3
Quick Back Pressure Check
This section describes how to measure the backpressure to the suppressor. Install the system for
the application of choice.
!
1.
Measure the system pressure P1 with the suppressor powered
2.
Unplug the line from the “Eluent Out” port on the suppressor and measure
system pressure P2
Do not leave the port open for more than 2 minutes.
CAUTION
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031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 30 of 84
3 – Operation
3.
P1-P2 < 150 psi.
4.
Adjust the backpressure coils if needed to achieve the < 150 psi value. Refer
to 3.2.2 to measure the back pressure contribution from the back pressure coil.
3.3 Plumbing for the AutoSuppression Recycle Mode Operation
Figure 5
The Auto Suppression Recycle Mode Plumbing Diagram
1/8 o.d. tubing
Injection Valve
Gas Separator
Waste Tube
Guard Column
Waste Container
Backpressure Coils
(Carbonate or Bicarbonate Eluents)
Analytical Column
1/8 o.d. tubing
1/16 o.d. tubing
Cell
As the eluent passes through the suppressor, it is neutralized to produce its weakly ionized form.
After passing through the conductivity cell, this effluent can be redirected to the regenerant inlet
on the suppressor, thus supplying it with a source of water containing a small amount of diluted
analyte (see Figure ). The main advantage of this mode is its simplicity and ease of use. It is not
necessary to have an external supply of water available for the suppressor.
!
CAUTION
Thermo Scientific
031956-11
Only use the AutoSuppression Recycle Mode for eluents and samples without organic
solvents or metallic contaminants such as iron in ground water.
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 31 of 84
3 – Operation
3.3.1
Eluent Flow Path Connections in the AutoSuppression Recycle Mode
Depending on the specific components (analytical column, conductivity cell, back pressure
coils) in the system, ¼-28 or 10-32 ferrule/bolt liquid lines may be required. All necessary
tubing and fittings are supplied in the detector or Dionex RFC-10 or Dionex RFC-30 Ship Kits.
To purchase or assemble ¼-28 or 10-32 ferrule/bolt liquid lines, refer to, “Dionex Liquid Line
Fittings.” Always use 0.005” i.d. PEEK tubing with 10-32 ferrule/bolt fittings on 2 mm
systems. Use 0.010” i.d. PEEK tubing with 10-32 ferrule/bolt fittings on 4 mm systems when
possible. Avoid adding dead volume to the system by keeping all eluent lines as short as
possible.
A. Install the Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500 Carbonate inside
the Dionex ICS Module.
B. Connect the outlet of the analytical column to the ELUENT IN of the suppressor
(see Figure ).
C. Connect the ELUENT OUT port of the suppressor to the inlet of the conductivity cell
(see Figure ).
3.3.2
Regenerant Flow Path Connections in the AutoSuppression Recycle Mode
Connect the back pressure coil(s) between the CELL OUTLET port and the REGEN IN port
(see Figure and Section 2). The back pressure coils are provided in the Gas Separator Waste
Tube Components Assembly (Item # 045825) for 4 mm systems. The backpressure coils for 2
mm systems are provided in the microbore tubing kit (Item # 052324) (see Figure 5 and Section
2). The back pressure coils are provided in the Gas Separator Waste Tube Components
Assembly (Item # 045825) for 4 mm systems. The backpressure coils for 2 mm systems are
provided in the microbore tubing kit (Item # 052324).
!
CAUTION
3.3.3
The Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500 Carbonate must be operated
with the Gas Separator Waste Tube (Item # 045460).
Installation in Thermal Chamber
A. Installation instructions for Dionex ICS-3000/5000/5000+ DC.
1.
Install the suppressor using the suppressor holder on the CD in the upper
compartment. Ensure the upper compartment temperature is set to a value no
greater than 40°C and temperature control is turned on. It is recommended that the
upper compartment is kept cooler than the lower compartment. If using a single
zone DC, do not set the temperature above 40°C. For best noise performance the
upper compartment can be set to 20° C.
B. Installation instructions for Dionex Integrion:
2.
Thermo Scientific
031956-11
Install the suppressor using the suppressor holder on the Conductivity Detector in
the detector compartment (bottom right door). If the Dionex Integrion is fitted with
detector compartment temperature control, ensure the detector compartment
temperature is set to a value less than 40°C and temperature control is turned on. It
is recommended that the detector compartment is kept cooler than the column
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
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3 – Operation
compartment (left door). For best noise performance the detector compartment can
be set to 20° C.
C. Installation instructions for Dionex Aquion, Dionex ICS 1100/1600/2100, AS50
thermal chamber TC and CC:
1.
Install the suppressor using the suppressor holder. The suppressor is installed
outside the heated column enclosure. It can support all high temperature
applications up to 60° C. Add a length of tubing (up to 20”, 50 cm) between the
column outlet and the suppressor inlet to allow time for the eluent to cool to room
temperature if operating the column above 35°C.
D. Installation instructions for LC30 and LC25 ovens:
!
1.
For all ANION and CATION applications up to 40° C, install the suppressor in the
oven using the suppressor holder.
2.
For operation above 40° C and up to 60° C, it is recommended that the suppressor
be installed outside the oven; this ensures optimal performance of the suppressor in
terms of noise and background. The Dionex CERS 500 and Dionex CERS 500e
suppressors are fully compatible with operation up to 60° C. The noise
performance would be slightly inferior at 60° C versus 30° C.
For best performance and suppressor longevity, the suppressor should be kept as cool as
possible; 20° C is ideal.
NOTE
Set the required current based on your specific application requirements for column flow rate
and temperature in addition to eluent concentration. If using Chromeleon software, the Wizards
can greatly assist you in determining the correct requirements. Refer to Section 2.6.1 for
suppressor current calculations and Appendix A for examples.
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031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 33 of 84
3 – Operation
3.4 Plumbing for the AutoSuppression External Water Mode Operation
Figure 6
The AutoSuppression External Water Mode Plumbing Diagram
1/4-28 Tubing i.d.
Injection
Valve
Guard
Column
Gas
Separator
Waste
Tube
To
Waste
Backpressure
Coil #2
Analytical
Column
Pressurized
Deionized
Water Source
Backpressure
Coil #1
Signal to
Conductivity
Detector
Any analysis that can be performed using the AutoSuppression Recycle Mode can be done
using the AutoSuppression External Water Mode. A constant source of deionized water having
a specific resistance of 18.2 megohm or greater is supplied to the regenerant chambers to
generate hydronium or hydroxide ions for neutralization.
!
AutoSuppression External Water Mode is used when organic solvents or metallic
contaminants are present in the eluent or sample, or borate is used as the eluent.
NOTE
!
NOTE
Thermo Scientific
031956-11
Because of its simplicity and equivalent performance, Thermo Fisher Scientific
recommends the use of Recycled Eluent Mode unless organic solvents or metallic
contaminants are present in the eluent or sample, or borate is used as the eluent.
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 34 of 84
3 – Operation
3.4.1
Eluent Flow Path Connections for the AutoSuppression External Water Mode
Depending on the specific components in the system (such as analytical column, conductivity
cell, and back pressure coils), ¼-28 or 10-32 ferrule/bolt liquid lines may be required. All
necessary tubing and fittings are supplied in the detector, or Dionex RFC-10, or Dionex RFC-30
Ship Kits. To purchase or assemble ¼-28 or 10-32 ferrule/bolt liquid lines, refer to, “Dionex
Liquid Line Fittings.” Always use 0.005” i.d. PEEK tubing with 10-32 ferrule/bolt fittings on
2 mm systems. When possible, use 0.010” i.d. PEEK tubing with 10-32 ferrule/bolt fittings on
4 mm systems. Avoid adding dead volume to the system by keeping all eluent lines as short as
possible.
A. Install the Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500 Carbonate in the
first slot inside the Chromatography Module.
B. Connect the outlet of the analytical column to the ELUENT IN of the suppressor
(see Figure ).
C. Connect the ELUENT OUT port of the suppressor to the inlet of the conductivity cell
(see Figure ).
D. Install a waste line from the conductivity cell that generates 40 psi back pressure at the
flow rate required by the application. Use the appropriate i.d. tubing depending on
your application requirements. Refer to Section 2.4 and see Table 3 for the correct
back pressure tubing requirements.
Install and adjust the flow rate of water from the pressurized water delivery system to the
regenerant chambers of the suppressor (see Table 6).
3.4.2
Regenerant Flow Path Connections in the AutoSuppression External Water Mode
The Dionex ERS 500 Pressurized Bottle Installation Kit (Item # 038018) contains all of the
components needed to install and operate the Dionex ERS 500, Dionex ERS 500e or Dionex
AERS 500 Carbonate with a pressurized water reservoir. The kit contains the Dionex ERS
Installation Parts Kit (Item # 039055), a 25 psi regulator (Item # 038201), and a 4 liter water
reservoir (Item # 039164).
A. Make the following air line connections:
1.
Locate the pieces of tinted 1/8” o.d. plastic tubing (Item # 030089) supplied in the
Installation Parts Kit.
2.
Push the end of one piece of 1/8” o.d. tubing over the barbed fitting of the
regulator. Connect the other end of the tubing to the source of air pressure.
3.
Push one end of the second piece of 1/8” o.d. tubing over the other barbed fitting of
the regulator. Push the other end of this tubing over the barbed fitting (Item #
030077) in the pressure inlet of the plastic reservoir (see Figure ).
B. Make the following water line connection.
1.
Thermo Scientific
031956-11
Use a coupler (Item # 039056) to connect one end of the 30” tubing assembly
(Item # 035727) that comes in the Installation Kit to the water reservoir. Connect
the other end of this tubing to the REGEN IN port of the suppressor.
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 35 of 84
3 – Operation
2.
Using a coupler (Item # 039056) and a 1/8” o.d. piece of tubing (Item # 035728)
from the Installation Kit, connect one end of this line to the REGEN OUT port of
the suppressor and then connect the other end of the line to the Gas Separator
Waste Tube.
C. Fill the water source reservoir. Make sure that the O-ring is inside the cap of the
reservoir before screwing the cap onto the reservoir. Screw the cap onto the reservoir
tightly and place the reservoir near the Chromatography Module.
D. With current applied, adjust the external water flow rate to match the eluent flow rate
by using a graduated cylinder and measuring the flow from the REGEN OUT waste
line. The pressure applied to the reservoir can vary from 0–25 psi (the lower and upper
pressure limits of the water reservoir) but the typical operating pressure is between 10
–15 psi for the Dionex ERS 500, or 5 – 10 psi for the Dionex ERS 500e or Dionex
AERS 500 Carbonate suppressor. Please note that this value is highly system
dependent and may vary from one suppressor to the next. In summary, the final
external water flow rate is dependent on two factors: the pressure applied to the water
reservoir and the current setting. It is best to measure it with the current on since the
application of current can affect the final flow rate significantly.
A safety relief valve on the reservoir regulator prevents pressure greater than 25 psi from
being applied to the water reservoir.
!
SAFETY
Table 6
Recommended External Water Flow Rate for Dionex ERS 500
Recommended External Water Flow Rate
Maximum External Water Flow Rate
Recommended External Flow Rate
(Neutralization Mode)
Table 7
Recommended External Water Flow Rate for Dionex ERS 500e and Dionex
AERS 500 Carbonate
Recommended External Water Flow Rate
Maximum External Water Flow Rate
3.4.3
Equal to the Eluent Flow Rate
2 mL/min (2 mm suppressors)
5 mL/min (4 mm suppressors)
5 mL/min (4 mm suppressor only)
At least 2x the eluent flow rate
2 mL/min (2 mm suppressors)
5 mL/min (4 mm suppressors)
Regenerant Flow Path Connections in the AutoSuppression External Water Mode with
Peristaltic Pump
For peristaltic pump plumbing refer to the “MASTERFLEX® C/L® Peristaltic Pump Quick Start
Guide” (Item # 065203). For the Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500
Carbonate plumbing refer to Figure , The AutoSuppression External Water Mode Plumbing
Diagram and pump the Deionized water into the “Regen in” port of the suppressor using the
peristaltic pump at the same flow rate as the eluent flow rate, see Table 6.
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 36 of 84
3 – Operation
3.4.4
Installation in Thermal Chamber
Refer to Section 3.3.3.
3.4.5
Dionex SRD-10 Suppressor Regenerant Detector
The Dionex SRD-10 Suppressor Regenerant Detector is a stand-alone device that monitors
liquid flow to a suppressor. If the flow is restricted or stops, the Dionex SRD-10 automatically
disables the eluent pump via a TTL command, thus preventing irreversible damage being done
to the suppressor. In the external water mode, if the water runs out in the source reservoir then
the suppressor would be powered without any regenerant flow; this condition results in
irreversible damage to the suppressor. Installing the Dionex SRD-10 suppressor regenerant
detector avoids this issue, as the system is turned off if the regenerant flow is interrupted either
due to a plumbing leak or due to the source reservoir becoming empty.
3.5 Plumbing for Chemical Suppression Mode Operation
The Dionex ERS 500 is not compatible with the chemical suppression mode. Therefore Thermo
Scientific Dionex recommends the Dionex CRS 500 or equivalent for chemical suppression
mode applications.
The Dionex ERS 500e and Dionex AERS 500 Carbonate can be used in the Chemical
Suppression Mode; however the total suppression capacity is reduced compared to a Chemically
Regenerated Suppressor. Thermo Scientific recommends the use of a Chemically Regenerated
Suppressor such as the Dionex CRS 500 for chemical suppression applications.
!
The Dionex ERS 500 is not compatible with the Chemical Suppression Mode. The Dionex
CRS 500 or equivalent should be used when Chemical Suppression Mode is required.
NOTE
3.6 Plumbing for MPIC Suppression Mode of Operation
The Dionex AERS 500e can be used with current applied to the suppressor augmented by dilute
sulfuric acid in the regenerant solution. If you intend to use an AutoRegen Accessory, configure
the system as described in Section 3.4, “Plumbing for the AutoSuppression External Water
Mode Operation,” and use the appropriate sulfuric acid concentration instead of water. The
Dionex CERS 500e can be used with current applied to the suppressor augmented by diluted
boric acid in the regenerant solution.
!
The following installation instructions are based on a system configured with a pressurized
water delivery system, but apply equally well to peristaltic pump delivery systems.
NOTE
!
The Dionex ERS 500 suppressor is not recommended for use with the MPIC Suppression
Mode of Operation. The Dionex ERS 500e should be used.
NOTE
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 37 of 84
3 – Operation
!
The Dionex AERS 500 Carbonate suppressor is not compatible with the MPIC Suppression
Mode of Operation.
NOTE
3.6.1
Eluent Flow Path Connections in MPIC Suppression Mode
To operate a system in the MPIC Suppression Mode, configure the system as described in
Section 3.4.1, “Eluent Flow Path Connections in the AutoSuppression External Water Mode.”
3.6.2
Regenerant Flow Path Connections in MPIC Suppression Mode Using Pressurized Water
Delivery System
The Dionex ERS Installation Kit (Item # 038018) contains all of the components needed to
install and operate the Dionex ERS 500e with a pressurized regenerant reservoir. The kit
contains the Dionex ERS Installation Parts Kit (Item # 039055), a 25 psi regulator (Item #
038201), and a 4-liter regenerant reservoir (Item # 039164).
To operate a system in the MPIC Suppression Mode, configure the system and use the
appropriate sulfuric acid concentration as described in Section 3.6.3.
3.6.3
MPIC Suppression Mode Operation
A. Anion MPIC
The Dionex AERS 500e can be used for suppression of MPIC (ion-pairing) eluents by
using the MPIC Suppression Mode. The MPIC Suppression Mode is a combination of
the AutoSuppression External Water Mode augmented with a chemical regenerant such
as sulfuric acid (H2SO4). When the Dionex AERS 500e is operating in this mode, it
uses an applied current and a constant source of dilute sulfuric acid solution from a
pressurized bottle delivery system or additional pump.
Table 8, “ Matching the Current Setting and Regenerant Flow Rate to the Eluent
Concentration and Flow Rate,” lists the eluent concentrations and flow rates of standard
eluents used in Anion MPIC separations and the current level and regenerant flow rate
required to suppress them.
!
The Dionex ERS 500 suppressor is not recommended for use with the MPIC Suppression
Mode of Operation. The Dionex ERS 500e should be used.
NOTE
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 38 of 84
3 – Operation
Table 8
Eluent
0.1–2.0 mM TBAOH
2.0–5.0 mM TBAOH
Matching the Current Setting and Regenerant Flow Rate to the Eluent
Concentration and Flow Rate for the Dionex AERS 500e (4 mm) in the MPIC
Suppression Mode
Eluent Flow Rate
(mL/min)
Current
(mA)
Regenerant Flow
Rate (mL/min)*
0.5–2.0
0.5–1.0
1.1–1.5
50/100
100/300
300/500
3–5
3–5
3–5
Regenerant
Concentration
(mN)
5–10
10
10
*Measured with power ON using a graduated cylinder
!
NOTE
For lower eluent concentration in a given range, choose lower corresponding current; for
higher eluent concentration, choose higher current setting. Higher current settings require
higher pressures applied to the pressurized regenerant delivery bottle to maintain adequate
regenerant flow.
B. Cation MPIC
The Dionex CERS 500e can be used for suppression of MPIC (ion-pairing) eluents by
using the AutoSuppression External Water Mode or the MPIC Suppression Mode
depending on the specific MPIC application. The MPIC Suppression Mode is a
combination of the AutoSuppression External Water Mode augmented with a chemical
regenerant if necessary, such as boric acid (H3BO3). When the Dionex CERS 500e is
operating in this mode, it uses an applied current and a constant source of dilute boric
acid solution from a pressurized bottle delivery system.
The separation of alkanolamines by ion-pairing using the Dionex CERS 500e requires
adding 10 mM boric acid to the regenerant to increase the ionization of the
ethanolamines, thereby increasing the conductivity of the alkanolamines. Boric acid
regenerant should not be used for the separations of the fully ionized alkali and alkaline
earth metals as the borate will displace the hydroxide counter ion and reduce the
conductance of these ions. Table 9, “ Matching the Current Setting and Regenerant
Flow Rate to the Eluent Concentration and Flow Rate ,” lists the eluent concentrations
and flow rates of standard eluents used in cation MPIC separations and the current level
and regenerant flow rate required to suppress them.
!
The Dionex ERS 500 suppressor is not recommended for use with the MPIC Suppression
Mode of Operation. The Dionex ERS 500e should be used.
NOTE
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 39 of 84
3 – Operation
Table 9
Matching the Current Setting and Regenerant Flow Rate
to the Eluent Concentration and Flow Rate Dionex CERS 500 (4 mm)
Eluent
0.1–2.0 mM Hexanesulfonic acid
(HSA)
2.0–5.0 mM Hexanesulfonic acid
0.1–2.0 mM Octane sulfonic acid (OSA)
2.0–5.0 mM OSA
0.1–2.0 mM Nonafluoropentanoic acid
2.0–5.0 mM Nonafluoropentanoic acid
Eluent Flow Rate
(mL/min)
0.5–1.0
Current
(mA) 1
50/100
Regenerant Flow
Rate (mL/min) 2
3–5
0.5–1.0
0.5–1.0
0.5–1.0
0.5–1.0
0.5–1.0
100/300
50/100
100/300
50/100
100/300
3–5
3–5
3–5
3–5
3–5
1. CERS 500 applications will operate best at current settings of 300 mA or lower.
Operating the CERS 500 at current settings over 300 mA can reduce the life of the suppressor and produce unnecessary baseline noise.
2. Measured with power ON using a graduated cylinder
!
NOTE
For the lower eluent concentration in a given range, choose the lower corresponding
current; for the higher eluent concentration, choose the higher current setting. Higher
current settings require higher pressures applied to the pressurized regenerant delivery
bottle to maintain adequate regenerant flow. Organic eluent solvents levels should be kept
below 40%.
3.7 Plumbing for Neutralization Mode Operation
For detailed information on installation of the Dionex ERS 500e on Dionex ICS systems for
Neutralization Mode, refer to Thermo Scientific Dionex Application Notes 93 and 94.
!
The Dionex ERS 500 suppressor is not recommended for use with the Neutralization Mode
of Operation. The Dionex ERS 500e should be used.
NOTE
Figure 7
Thermo Scientific
031956-11
The Dionex ERS 500e Neutralization Mode Plumbing Diagram
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 40 of 84
3 – Operation
3.7.1
Sample Liquid Line Connections for Dionex ERS 500e in Neutralization Mode
A. Install the Dionex ERS 500e in the chromatography module.
B. Connect the eluent line from Port 1 on the Recycle Valve to the ELUENT IN port of
the Dionex ERS 500e.
C. Connect the ELUENT OUT port of the Dionex ERS 500e to Port 5 of the Recycle
Valve.
3.7.2
Regenerant Liquid Line Connections for Dionex ERS 500e in Neutralization Mode
The SP10 AutoNeutralizer Ship Kit (Item # 047950) contains all the components needed to
install and operate the Dionex ERS 500e in Neutralization Mode with a pressurized water
reservoir.
A. Make the following air line connections.
1.
Locate the pieces of tinted 1/8” OD plastic tubing (Item # 030089) supplied in the
Ship Kit.
2.
Push the end of piece of 1/8” OD tubing over the barbed fitting of the regulator.
Connect the other end of the tubing to the source of air pressure.
3.
Push one end of the second piece of 1/8” OD tubing over the other barbed fitting of
the regulator. Push the other end of this tubing over the barbed fitting (Item # 030077)
in the pressure inlet of the plastic reservoir.
B. Make the following liquid line connections:
3.7.3
1.
Use a coupler (Item # 039056) to connect one end of the 30” tubing assembly (Item #
035727) that comes in the AutoNeutralization Ship Kit to the water reservoir. Connect
the other end of this tubing to the REGEN IN port of the Dionex ERS 500e.
2.
Using a coupler (Item # 039056) and a 1/8” OD piece of tubing (Item # 035728) from
the AutoNeutralization Ship Kit, connect one end of this line to the REGEN OUT port
of the Dionex ERS 500 and then connect the other end of the line to the Gas Separator
Waste Tube.
3.
Fill the reservoir with water. Make sure the o-ring is inside the cap of the reservoir
before screwing the cap onto the reservoir. Screw the cap onto the reservoir tightly
and place the reservoir near or on top of the instrument in a secondary waste container
such as the Dionex EO.
Pump Trap Column Installation and Regeneration
A. Installation of the Dionex ATC-HC Trap Column
The Dionex ATC-HC Trap Column (Item # 059604) is used to trap anions that may be in
the carrier solution that could result in high blanks. The Dionex ATC-HC should be
regenerated prior to installation.
B. Regeneration of the Dionex ATC-HC Trap Column
Using a pump other than the carrier pump, the Dionex ATC-HC should be regenerated and
rinsed using the following two steps.
1.
Thermo Scientific
031956-11
Regenerate the Dionex ATC-HC with 0.5 M NaOH for 50 minutes at a flow rate of 1
mL/min.
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 41 of 84
3 – Operation
2.
Rinse the Dionex ATC-HC with deionized water for 30 minutes at a flow rate of 1
mL/min. If the carrier solution is different from water, equilibrate it to the carrier
solution for 30 minutes at a flow rate of 1 mL/min.
C. Installation of the Dionex CTC-1 Trap Column
The Dionex CTC-1 Trap Column (Item # 040192) is used to trap cations that may be in the
carrier solution that could result in high blanks. The Dionex CTC-1 should be regenerated
prior to installation.
D. Regeneration of the Dionex CTC-1 Trap Column
Using a pump other than the carrier pump, the Dionex CTC-1 should be regenerated and
rinsed using the following two steps.
3.7.4
1.
Regenerate the Dionex CTC-1 with 0.5 M H2SO4 for 50 minutes at a flow rate of 1
mL/min.
2.
Rinse the Dionex CTC-1 with deionized water for 30 minutes at a flow rate of 1
mL/min. If the carrier solution is different from water, equilibrate it to the carrier
solution for 30 minutes at a flow rate of 1 mL/min.
Neutralization Mode Operation
For detailed operation of Dionex IC systems, consult the accompanying Product Manuals.
!
The Dionex ERS 500 suppressor is not recommended for use with the Neutralization Mode
of Operation. The Dionex ERS 500e should be used.
NOTE
Neutralization pretreatment coupled with Ion Chromatography involves the following steps:
A. The concentrated sample is loaded into the sample loop of the “Sample Valve” with
either a syringe or an Autosampler.
B. The sample loop is switched in-line and flushed with a stream of deionized water.
C. The deionized water pushes the sample through the Dionex ERS 500, where
neutralization begins.
D. Common bases or acids that have been diluted fourfold or more can be completely
neutralized after one pass through the Dionex ERS 500e at flow rates ≤ 0.5 mL.min.
E. For more concentrated bases or acids or faster flow rates, multiple passes through the
Dionex ERS 500e will be required for complete neutralization.
F. The partially neutralized sample is passed down to the 2 mL collection loop on the
Recycle Valve.
G. The recycle valve is actuated so that the sample flow is directed to pass through the
Dionex ERS 500e a second time (or multiple times) to neutralize the sample
completely.
H. The Recycle Valve can be actuated multiple times if multiple passages are necessary
for complete neutralization.
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 42 of 84
3 – Operation
I.
After complete neutralization, the sample is delivered to the injection valve of the
Dionex Ion Chromatography system and a low pressure trap column such as the
Dionex UTAC-XLP2 (Item # 072781) for the Dionex AERS 500e (4 mm) or Dionex
TCC-XLP1 (Item # 063889) for the Dionex CERS 500e (4 mm).
J.
The trace ions are then analyzed by the Dionex Ion Chromatography system.
For detailed information on the timing and configuration of the Sample Valve, Recycle Valve,
and Injection Valve, refer to Thermo Scientific Dionex Application Note 93 or 94, or to the
Dionex SP10 AutoNeutralizer Operator’s Manual (Document Number 034980).
3.8 Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500 Carbonate Storage
The Dionex ERS 500, Dionex ERS 500e and Dionex AERS 500 Carbonate are shipped with
deionized water as the storage solution. If the suppressor will not be used for more than three
days, prepare it for storage. The resin, screens and membranes in the suppressor must be
completely hydrated to maintain liquid seal and chromatographic performance. Plug all of the
ports after hydration.
3.8.1
Short Term Storage (3 to 7 days)
A. Using a plastic syringe, push 5 mL of deionized water through the REGEN IN port,
and 3 mL of Deionized Water through the ELUENT IN port until all bubbles are
removed. Plug all the ports (both REGEN and ELUENT ports).
B. To resume operation, connect the suppressor to the system. Allow the system to
equilibrate before starting analysis.
!
NOTE
3.8.2
If the eluent last used contained organic solvents, flush the Dionex ERS 500, Dionex ERS
500e or Dionex AERS 500 Carbonate with deionized water for 10 minutes through both
chambers before plugging the fitting ports.
Long Term Storage (More than 7 days)
A. Connect the eluent and regenerant chambers in series and flush the Dionex ERS 500,
Dionex ERS 500e or Dionex AERS 500 Carbonate with deionized water for 10
minutes at 1.0 mL/min (4 mm) or 0.25 mL/min (2 mm).
B. Plug all suppressor ELUENT ports and REGEN ports.
C. To resume operation, complete the hydration steps and connect the suppressor to the
system. Allow the system to equilibrate before starting analysis.
D. If small or increasing analyte peak areas are observed, perform steps A–G as outlined
in Section 5.2, “Small or increasing Analyte Peak Area or Regeneration.”
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 43 of 84
4 – Example Applications
4. Example Neutralizer Mode Applications
The following examples are presented to highlight the analysis of trace anions or cations found
in the concentrated matrices that have been treated using a Dionex Electrolytically Regenerated
Suppressor (Dionex ERS 500e) in Neutralizer Mode.
The analysis may be done on any one of a number of Dionex anion or cation exchange column
sets. Please refer to the Product Manual for the particular anion or cation exchange column set
installed in your system for detailed operation and troubleshooting information.
Because of its low operating pressure, the Dionex UTAC-XLP2 (Item # 072781) or Dionex
TCC-XLP1 (Item # 063889) should be used to concentrate the trace anions or cations in the
sample neutralized by the Dionex ERS 500e. The use of other concentrators may result in
excessive back pressure to the Dionex ERS 500e, thus damaging the Dionex ERS 500e.
!
The Dionex ERS 500 suppressor is not recommended for use with the Neutralization Mode
of Operation. The Dionex ERS 500e should be used.
NOTE
4.1 System Blank
4.1.1
Anion System Blank
Trace anion contamination in the deionized water used for the carrier solution, the Dionex
AERS 500e and eluent create the analytical blank. For trace analysis, the analytical blank
usually determines the detection limits of the system. Anion contaminants in deionized water
can be removed by installing Dionex IonPac ATC-HC column between the sample carrier pump
and the Sample Valve. The major source of sulfate contamination is usually from the Dionex
AERS 500e. Since this device uses high capacity surface sulfonated ion exchange screens and
membranes, sulfate is released from the screen and membrane surfaces, especially in a newly
installed Dionex AERS 500e.
To reduce the sulfate blank, follow the Dionex ERS 500e startup procedure in Section 3.3,
“Start-up Procedure for the Dionex Electrolytically Regenerated Suppressor (Dionex ERS
500e).” The sulfate blank is normally reduced to a constant level after 24 hours of operation.
Typical blanks are shown in Figure , “Dionex IonPac AS11 Gradient Analysis of Blank,
Standard, 10% NaOH Sample and spiked 10% NaOH Sample” and Figure , “Dionex IonPac
AS12A Isocratic Analysis of Blank, Standard, 25% NaOH Sample and Spiked 25% NaOH
Sample.” Finally, anion contaminants in the eluent (e.g., Na2CO3/NaHCO3 and NaOH) also
contribute to the analytical blank. A choice of high purity chemicals helps to reduce the blank
concentrations.
4.1.2
Cation System Blank
Trace cation contamination in the deionized water used for the carrier solution, the Dionex
CERS 500e and eluent create the analytical blank. For trace analysis, the analytical blank
usually determines the detection limits of the system. Cation contaminants in deionized water
can be removed by installing Dionex CTC-1 Column between the sample carrier pump and the
Sample Valve. The major source of ammonia and amine contamination is usually from the
Dionex CERS 500e. Since this device uses high capacity aminated ion exchange screens and
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 44 of 84
4 – Example Applications
membranes, ammonia and amines are released from the screen and membrane surfaces,
especially in a newly installed Dionex CERS 500e.
To reduce the amine blank, follow the Dionex ERS 500e start-up procedure in Section 3.2,
“Start-up Procedure for the Dionex Electrolytically Regenerated Suppressor (Dionex ERS
500).” The amine blank is normally reduced to a constant level after 24 hours of operation. A
typical blank is shown in Figure , “Dionex IonPac CS12A Blank and Standard Analysis.” The
use of high purity chemicals helps to reduce the blank concentrations.
4.2 Dionex CERS 500e Conditioning
Since the Dionex CERS 500e is a high-capacity anion exchange device which supplies the high
concentration of hydroxide for acid neutralization, the hydrolysable ions such as Mg2+ and Ca2+
in hydroxide forms may be precipitated in the Dionex CERS 500e. In general, when standard
cation in water solutions are employed for system calibration, it is possible that Mg2+ and Ca2+
may be precipitated due to the high hydroxide concentration. These cations are then “carried
over” to the first acid run. To avoid the hydrolysis of the alkaline earth metals during standard
calibration, the Dionex CERS 500e is “conditioned” by running a complete pretreatment cycle
of acid (preferably 24% sulfuric acid) prior to standard runs. Follow the Dionex CERS 500e
acid treatment procedure below prior to each standard run.
A. Confirm that the system functions properly.
B. Set “Sample Valve” to Load Position and load 24% sulfuric acid.
C. Switch the sample valve to Inject position and flush the sample loop with deionized
water.
D. After the sulfuric acid passes through the neutralizer and IC injection valve
(approximately 4–6 minutes with the collection coil installed or 1-2 minutes without
the collection coil installed), the system is ready for standard injection.
The above steps are applied only when standard calibration is performed. However, if the
standard injection is made immediately after the sample runs (acid samples) the Dionex CERS
500e acid pretreatment is not required.
If the standard run is not started within 20 minutes after the acid calibration step, the overall
process must be repeated. When multiple point calibration is performed, the Dionex CERS 500e
acid pretreatment can be made any time after the beginning of the analytical separation.
4.3 System Calibration
The analytical blank should be incorporated into the calibration curve. One or two level
standards are usually required to calibrate the Ion Chromatograph. For trace analysis, typical
standard concentrations are 2 to 5 times sample concentrations. For example, for anions, if the
sample contains 50 ppb each of Cl–, NO3– and SO42–, the standard calibration should not exceed
250 ppb each of these anions. For cations, if the sample contains 10 ppb each of K +, Mg2+ and
Ca2+, the standard calibration should not exceed 50 ppb each of these cations.
4.3.1
Analysis of Acid Samples Containing High Concentrations of Transition Metals
If a sample contains transition metals in ppm (mg/L) levels, these elements may interfere with
cation detection by suppressed conductivity. The additional of complexing agent such as
pyridine-2,6-dicarboxylic acid (PDCA, Item # 039671) to the CS12A eluent is required to
selectively remove transition metals from the eluent via the suppression system.
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 45 of 84
4 – Example Applications
Detection Limits of Dionex ERS 500e Sample Pretreatment and Subsequent Ion
Chromatography
Anion
ppb*
Chloride
Bromide
Chlorate
Nitrate
Phosphate
Sulfate
Oxalate
4
20
30
20
50
30
50
Cation
ppb*
Lithium
Sodium
Potassium
Magnesium
Calcium
0.03
1.0
2.0
0.6
0.8
* Estimated values in H2SO4 matrix
* Estimated values in NaOH matrix
4.4 Recovery Data for Trace Anions in 25% Sodium Hydroxide
Anion
25% NaOH*
(ppb)
Spike
(ppb)
Expected
(ppb)
Found*
(ppb)
Chloride
Bromide
Chlorate
Nitrate
Phosphate
Sulfate
Oxalate
155 + 3
—
—
11 + 6
106 + 25
348 + 4
134 + 28
100
100
100
100
100
200
100
255
100
100
111
206
548
234
258 + 4
99 + 8
96 + 9
119 + 13
183 + 17
553 + 11
238 + 22
*n=8
Separation Conditions are shown in Section 4.7, “Dionex IonPac AS12A Isocratic Analysis of 25% Sodium Hydroxide.”
4.5 Recovery Data for Trace Cations in 24% Sulfuric Acid
Cation
24% H2SO4*
(ppb)
Spike
(ppb)
Expected
(ppb)
Found*
(ppb)
Lithium
Sodium
Potassium
Magnesium
Calcium
--7.4 + 0.4
3.3 + 0.5
2.0 + 0.2
10.6 + 0.1
2.0
8.0
20.0
10.0
20.0
2.0
15.4
23.3
12.0
30.6
1.993 + 0.008
14.3 + 0.1
22.5 + 0.2
12.2 + 0.2
33 + 1
*n=8
Separation Conditions are shown in Section 4.8, “Dionex IonPac CS12A Isocratic Analysis of 24% Sulfuric Acid”.
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 46 of 84
4 – Example Applications
4.6 Anion Example Applications
4.6.1
Dionex IonPac® AS11 Gradient
A.
Without Guard
Eluent 1:
Eluent 2:
Eluent 3:
Type I DI Water
5.0 mM NaOH
100 mM NaOH
TIME (min)
Equilibration
0
7.0
Analysis
0.0
0.2
2.0
5.0
15.0
B.
Eluent Flow Rate:
2.0 mL/min
% E1
% E2
%E3
Comments
90
90
10
10
0
0
0.5 mM NaOH for 7 min
90
90
90
0
0
10
10
10
100
65
0
0
0
0
35
0.5 mM NaOH, Inject
Inject Valve to Load Position
0.5-5.0 mM NaOH in 3 min
5.0-38.25 mM NaOH in 10 min
With Guard
Eluent 1: Type I DI Water
Eluent 2: 5.0 mM NaOH
Eluent 3: 100 mM NaOH
TIME (min)
Equilibration
0
7.0
Analysis
0.0
0.2
2.5
6.0
18.0
!
Eluent Flow Rate: 2.0 mL/min
% E1
% E2
%E3
Comments
90
90
10
10
0
0
0.5 mM NaOH for 7 min
90
90
90
0
0
10
10
10
100
65
0
0
0
0
35
0.5 mM NaOH, Inject
Inject Valve to Load Position
0.5-5.0 mM NaOH in 3.5 min
5.0-38.25 mM NaOH in 12 min
The steps for sample neutralization can be performed while the Thermo Scientific Dionex
IonPac AS11 is equilibrating with the starting eluent (90% E1/10% E2).
NOTE
Seven minutes are required at the beginning of the above program for equilibration of the
Dionex IonPac AS11 with E1 prior to injecting the next sample. If the system is not used
continuously, that is, the run program (equilibration plus analysis) is not started exactly every 22
minutes (without Dionex IonPac AG11) or 25 minutes (with Dionex IonPac AG11), the run
program can be modified to start with 2 minutes of the highest eluent concentration for
regeneration and then to equilibrate with E1 for 7 minutes with the next injection 9 minutes into
the program.
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 47 of 84
4 – Example Applications
4.6.2
Dionex IonPac AS11 Gradient Analysis of 10% Sodium Hydroxide
Sample:
Concentrator Column:
Trap Column:
Guard Column:
Analytical Column:
Eluents
Eluent Flow Rate:
Electrolytic Suppressor:
or Chemical Suppressor:
Chemical Regenerant:
Expected Background Conductivity:
Expected System Operating
Back Pressure:
Without Guard: 1,600 psi (11.03 MPa)
With Guard: 1,850 psi (12.75 MPa)
Figure 8
Thermo Scientific
031956-11
100 µL of 10% NaOH neutralized in the SP10 AutoNeutralizer
Low Pressure Trace Anion Concentrator
(i.e., Dionex UTAC-XLP2, Item # 072781)
Anion Trap Column (i.e., Dionex ATC-HC 500, Item # 075978)
Dionex IonPac AG11 Guard Column
Dionex IonPac AS11 Analytical Column
E1:
Type I Deionized Water
E2:
5.0 mM NaOH
E3:
100 mM NaOH
2.0 mL/min (4-mm)
Dionex Anion Electrolytically Regenerated Suppressor, Dionex AERS 500
Recycled Eluent Mode
Dionex Anion Chemically Regenerated Suppressor, Dionex ACRS 500
50 mN H2SO4
0.5 mM NaOH: 1 µS
35 mM NaOH: 3.5 µS
Dionex IonPac AS11 Gradient Analysis of Blank, Standard, 10% NaOH
Sample and Spiked 10% NaOH Sample
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 48 of 84
4 – Example Applications
Table 10
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
25.
26.
27.
28.
29.
30.
Quinate
Fluoride
Acetate
Propionate
Formate
Methylsulfonate
Pyruvate
Valerate
Monochloroacetate
Bromate
Chloride
Trifluoroacetate
Bromide
Nitrate
Chlorate
Selenite
Carbonate
Malonate
Maleate
Sulfate
Oxalate
Tungstate
Phthalate
Phosphate
Chromate
Citrate
Tricarballylate
Isocitrate
cis-Aconitate
trans-Aconitate
Recovery Data for the Dionex IonPac AS11 Analysis of 10% NaOH
Standard
10% NaOH
Expected
Spiked Value
10% NaOH
Spiked
Conc.
(mg/L)
Conc.
(mg/L)
Conc.
(mg/L)
Conc.
(mg/L)
1.000
0.200
1.000
1.000
1.000
1.000
1.000
1.000
1.000
1.000
0.400
1.000
0.600
0.600
0.600
1.000
--1.000
1.000
1.000
1.000
2.000
2.000
2.000
2.000
2.000
2.000
2.000
2.000
2.000
ND
0.030
0.081
ND
0.213
ND
ND
ND
ND
ND
8.750
ND
ND
0.048
1.178
ND
--ND
ND
9.842
0.166
ND
ND
ND
ND
ND
ND
ND
ND
0.072
1.000
0.230
1.081
1.000
1.213
1.000
1.000
1.000
1.000
1.000
9.150
1.000
0.600
0.648
1.778
1.000
--1.000
1.000
10.842
1.166
2.000
2.000
2.000
2.000
2.000
2.000
2.000
2.000
2.072
1.435
0.244
1.301
1.069
1.216
1.070
1.103
1.009
0.811
1.036
9.095
1.055
0.640
0.657
1.870
0.990
--1.303
1.026
10.925
1.244
2.201
2.086
2.120
2.287
2.214
2.098
2.112
1.512
2.303
ND = None Detected
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 49 of 84
4 – Example Applications
4.6.3
Dionex IonPac AS12A Isocratic Analysis of 25% Sodium Hydroxide
Sample:
Concentrator Column:
Column:
Eluent:
Eluent Flow Rate:
Electrolytic Suppressor:
or Chemical Suppressor:
Chemical Regenerant:
Expected Background Conductivity:
Figure 5
Thermo Scientific
031956-11
100 µL of 25% NaOH neutralized in the SP10 AutoNeutralizer
Low Pressure Trace Anion Concentrator
(i.e., Dionex UTAC-XLP2, Item # 072781)
Dionex IonPac AG12A + Dionex IonPac AS12A
2.7 mM Na2CO3/0.3 mM NaHCO3
1.5 mL/min
Dionex Anion Electrolytically Regenerated Suppressor, Dionex AERS 500
Recycled Eluent Mode
Dionex Anion Chemically Regenerated Suppressor, Dionex ACRS 500
50 mN H2SO4
14–16 µS
Dionex IonPac AS12A Isocratic Analysis of Blank, Standard, 25% NaOH
Sample and Spiked 25% NaOH Sample
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 50 of 84
4 – Example Applications
Table 11
1.
2.
3.
4.
5.
6.
7.
Chloride
Bromide
Chlorate
Nitrate
Phosphate
Sulfate
Oxalate
Recovery Data for the Dionex IonPac AS12A Analysis of 25% NaOH
Standard
Spiking
Standard
25% NaOH
Expected
Spiked Value
Conc.
(mg/L)
Conc.
(mg/L)
Conc.
(mg/L)
Conc.
(mg/L)
0.200
0.200
0.200
0.200
0.200
0.400
---
0.100
0.100
0.100
0.100
0.100
0.200
0.100
0.155 + 0.003
ND
ND
0.011 + 0.006
0.106 + 0.025
0.348 + 0.004
0.134 + 0.028
0.255
0.100
0.100
0.111
0.206
0.548
0.234
25% NaOH
Spiked
Conc.
(mg/L)
0.258 + 0.004
0.099 + 0.008
0.099 + 0.009
0.199 + 0.013
0.183 + 0.017
0.553 + 0.011
0.238 + 0.022
Based on 8 runs
ND = None Detected
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 51 of 84
4 – Example Applications
4.6.4
Dionex IonPac AS12A Isocratic Analysis of 20% Ammonium Hydroxide
Sample:
Concentrator Column:
Column:
Eluent:
Eluent Flow Rate:
Electrolytic Suppressor:
or Chemical Suppressor:
Chemical Regenerant:
Expected Background Conductivity:
Figure 6
Thermo Scientific
031956-11
100 µL of 20% NH4OH neutralized in the SP10 AutoNeutralizer
Low Pressure Trace Anion Concentrator
(i.e., Dionex UTAC-XLP2, Item # 072781)
Dionex IonPac AG12A + Dionex IonPac AS12A
2.7 mM Na2CO3/0.3 mM NaHCO3
1.5 mL/min
Dionex Anion Electrolytically Regenerated Suppressor, Dionex AERS 500
Recycled Eluent Mode
Dionex Anion Chemically Regenerated Suppressor, Dionex ACRS 500
50 mN H2SO4
14–16 µS
Dionex IonPac AS12A Analysis of 20% Ammonium Hydroxide
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 52 of 84
4 – Example Applications
4.6.5
Dionex IonPac AS12A Isocratic Analysis of 25% Tetramethylammonium Hydroxide
Sample:
Concentrator Column:
Column:
Eluent:
Eluent Flow Rate:
Electrolytic Suppressor:
or Chemical Suppressor:
Chemical Regenerant:
Expected Background Conductivity:
Figure 7
Thermo Scientific
031956-11
100 µL of 25% TMAOH neutralized in the SP10 AutoNeutralizer
Low Pressure Trace Anion Concentrator
(i.e., Dionex UTAC-XLP2, Item # 072781)
Dionex IonPac AG12A + Dionex IonPac AS12A
2.7 mM Na2CO3/0.3 mM NaHCO3
1.5 mL/min
Dionex Anion Electrolytically Regenerated Suppressor, Dionex AERS 500
Recycled Eluent Mode
Dionex Anion Chemically Regenerated Suppressor, Dionex ACRS 500
50 mN H2SO4
14–16 µS
IonPac AS12A Analysis of 25% Tetramethylammonium Hydroxide
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 53 of 84
4 – Example Applications
4.6.6
IonPac AS12A Isocratic Analysis of 25% Tetrabutylammonium Hydroxide
Sample:
Concentrator Column:
Column:
Eluent:
Eluent Flow Rate:
Electrolytic Suppressor:
or Chemical Suppressor:
Chemical Regenerant:
Expected Background Conductivity:
Figure 8
Thermo Scientific
031956-11
100 µL of 25% TBAOH neutralized in the SP10 AutoNeutralizer
Low Pressure Trace Anion Concentrator
(i.e., Dionex UTAC-XLP2, Item # 072781)
Dionex IonPac AG12A + Dionex IonPac AS12A
2.7 mM Na2CO3/0.3 mM NaHCO3
1.5 mL/min
Dionex Anion Electrolytically Regenerated Suppressor, Dionex AERS 500
Recycled Eluent Mode
Dionex Anion Chemically Regenerated Suppressor, Dionex ACRS 500
50 mN H2SO4
14–16 µS
Dionex IonPac AS12A Analysis of 25% Tetrabutylammonium Hydroxide
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 54 of 84
4 – Example Applications
4.7 Cation Example Applications
4.7.1
Dionex IonPac CS12A Blank and Standard Analysis
Sample:
Concentrator Column:
Guard Column:
Analytical Column:
Eluent:
Eluent Flow Rate:
Electrolytic
Suppressor:
100 µL of Standard or Blank neutralized in the Dionex CERS 500
Low Pressure Trace Cation Concentrator (i.e. Dionex TCC-XLP1, Item # 063889)
Dionex IonPac CG12A Guard Column
Dionex IonPac CS12A Analytical Column
20 mM Methanesulfonic acid
1.0 mL/min
Dionex Cation Electrolytically Regenerated Suppressor, Dionex CERS 500
Recycled Eluent Mode
Dionex Cation Chemically Regenerated Suppressor, Dionex CCRS 500
100 mN TBAOH
20 mM MSA: <1 µS
or Chemical
Suppressor:
Chemical Regenerant:
Expected Background Conductivity:
Expected System Operating Back Pressure:
Without Guard: 1,400 psi (9.65 MPa)
With Guard: 1,850 psi (12.75 MPa)
Analyte
1.
2.
3.
4.
5.
6.
7.
8.
Figure 9
Dionex IonPac CS12A Blank and Standard Analysis
Figure 14A
Thermo Scientific
031956-11
Lithium
Sodium
Ammonium
Dimethylamine
Potassium
Trimethylamine
Magnesium
Calcium
Conc..
(µg/L)
5.0
20.0
25.0
B
50.0
B
25.0
50.0
Figure 14B
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 55 of 84
4 – Example Applications
4.7.2
Dionex IonPac CS12A Analysis of 24% Sulfuric Acid
Sample:
Concentrator Column:
Guard Column:
Analytical Column:
Eluent:
Eluent Flow Rate:
Electrolytic Suppressor:
or Chemical Suppressor:
Chemical Regenerant:
Expected Background Conductivity:
Expected System Operating Back Pressure:
Figure 10
100 µL of 24% sulfuric acid neutralized in the Dionex CERS 500
Low Pressure Trace Cation Concentrator
(i.e. Dionex TCC-XLP1, Item # 063889)
Dionex IonPac CG12A Guard Column
Dionex IonPac CS12A Analytical Column
20 mM Methanesulfonic acid (MSA)
1.0 mL/min
Dionex Cation Electrolytically Regenerated Suppressor, Dionex CERS 500
Recycled Eluent Mode
Dionex Cation Chemically Regenerated Suppressor, Dionex CCRS 500
100 mN TBAOH
20 mM MSA: <1 µS
Without Guard: 1,400 psi (9.65 MPa)
With Guard: 1,850 psi (12.75 MPa)
Dionex IonPac CS12A Analysis of 24% H2SO4 Sample
Figure 14A
24% H2SO4 Sample
Table 12
1.
2.
3.
4.
5.
6.
7.
8.
Lithium
Sodium
Ammonium
Dimethylamine
Potassium
Trimethylamine
Magnesium
Calcium
Figure 14B
Spiked 24% H2SO4 Sample
Recovery Data for the Dionex IonPac CS12A Analysis of 24% H2SO4
Spiking
Standard
Conc.
(µg/L)
24% H2SO4*
Unspiked Value
Conc.
(µg/L)
Expected
Spiked Value
Conc.
(µg/L)
24% H2SO4*
Spiked Value
Conc.
(µg/L)
2.0
8.0
B
B
20.0
B
10.0
20.0
ND
7.4 + 0.4
B
B
3.3 + 0.5
B
2.0 + 0.2
10.6 + 0.1
2.0
15.4
B
B
23.3
B
12.0
30.6
1.993 + 0.008
14.3 + 0.1
B
B
22.5 + 0.2
B
12.2 + 0.2
33 + 1
*
Based on 8 runs
ND = None Detected, B = Blank
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 56 of 84
4 – Example Applications
4.7.3
Dionex IonPac CS12A Analysis of 25% Acetic Acid
Sample:
Concentrator Column:
Guard Column:
Analytical Column:
Eluent:
Eluent Flow Rate:
Electrolytic Suppressor:
or Chemical Suppressor:
Chemical Regenerant:
Expected Background Conductivity:
Expected System Operating Back Pressure:
100 µL of 25% acetic acid neutralized in the Dionex CERS 500
Low Pressure Trace Cation Concentrator (i.e. Dionex TCC-XLP1, Item # 063889)
Dionex IonPac CG12A Guard Column
Dionex IonPac CS12A Analytical Column
20 mM Methanesulfonic acid (MSA)
1.0 mL/min
Dionex Cation Electrolytically Regenerated Suppressor, Dionex CERS 500
Recycled Eluent Mode
Dionex Cation Chemically Regenerated Suppressor, Dionex CCRS 500
100 mN TBAOH
20 mM MSA: <1 µS
Without Guard: 1,400 psi (9.65 MPa)
With Guard: 1,850 psi (12.75 MPa)
Analyte
1.
2.
3.
4.
5.
6.
7.
Figure 11
Thermo Scientific
031956-11
Sodium
Ammonium
Dimethylamine
Potassium
Trimethylamine
Magnesium
Calcium
Conc..
(µg/L)
86.7
33.7
B
21.6
B
2.5
133.0
Dionex IonPac CS12A Analysis of 25% Acetic Acid
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 57 of 84
4 – Example Applications
4.7.4
Dionex IonPac CS12A Analysis of 10% Hydrofluoric Acid
Sample:
Concentrator Column:
Guard Column:
Analytical Column:
Eluent:
Eluent Flow Rate:
Electrolytic Suppressor:
or Chemical Suppressor:
Chemical Regenerant:
Expected Background Conductivity:
Expected System Operating Back Pressure:
100 µL of 10% hydroflouric acid neutralized in the Dionex CERS 500
Low Pressure Trace Cation Concentrator (i.e. Dionex TCC-XLP1, Item # 063889)
Dionex IonPac CG12A Guard Column
Dionex IonPac CS12A Analytical Column
20 mM Methanesulfonic acid (MSA)
1.0 mL/min
Dionex Cation Electrolytically Regenerated Suppressor, Dionex CERS 500
Recycled Eluent Mode
Dionex Cation Chemically Regenerated Suppressor, Dionex CCRS 500
100 mN TBAOH
20 mM MSA: <1 µS
Without Guard: 1,400 psi (9.65 MPa)
With Guard: 1,850 psi (12.75 MPa)
Analyte
1.
2.
3.
4.
5.
6.
7.
8.
Lithium
Sodium
Ammonium
Dimethylamine
Potassium
Trimethylamine
Magnesium
Calcium
Conc.
(µg/L)
0.4
66.7
110.5
B
8.4
B
15.3
59.6
Figure 126 Dionex IonPac CS12A Analysis of 10% Hydrofluoric Acid
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 58 of 84
4 – Example Applications
4.7.5
Dionex IonPac CS12A Analysis of 22% Phosphoric Acid
Sample:
Concentrator Column:
Guard Column:
Analytical Column:
Eluent:
Eluent Flow Rate:
Electrolytic Suppressor:
or Chemical Suppressor:
Chemical Regenerant:
Expected Background Conductivity:
Expected System Operating Back Pressure:
100 µL of 22% phosphoric acid neutralized in the Dionex CERS 500
Low Pressure Trace Cation Concentrator (i.e. Dionex TCC-XLP1, Item # 063889)
Dionex IonPac CG12A Guard Column
Dionex IonPac CS12A Analytical Column
20 mM Methanesulfonic acid (MSA)
1.0 mL/min
Dionex Cation Electrolytically Regenerated Suppressor, Dionex CERS 500
Recycled Eluent Mode
Dionex Cation Chemically Regenerated Suppressor, Dionex CCRS 500
100 mN TBAOH
20 mM MSA: <1 µS
Without Guard: 1,400 psi (9.65 MPa)
With Guard: 1,850 psi (12.75 MPa)
Analyte
1.
2.
3.
4.
5.
6.
7.
Figure 13
Thermo Scientific
031956-11
Sodium
Ammonium
Dimethylamine
Potassium
Trimethylamine
Magnesium
Calcium
Conc..
(µg/L)
509.1
438.6
B
25.8
B
22.4
144.1
Dionex IonPac CS12A Analysis of 22% Phosphoric Acid
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 59 of 84
5 – Troubleshooting Guide
5. Troubleshooting Guide
The purpose of the Troubleshooting Guide is to help you solve operating problems that may
arise while using the Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500 Carbonate. For
more information on problems that originate with the Ion Chromatograph System or the specific
exchange column set in use, refer to the Troubleshooting Guide in the appropriate Product
Manual. If you cannot solve the problem on your own, contact the Thermo Fisher Scientific
Regional Office nearest you.
!
CAUTION
Do not allow eluent to flow through the Dionex ERS 500, Dionex ERS 500e or Dionex
AERS 500 Carbonate without the power turned on for more than a few minutes. Doing so
will cause noticeable reduction of analyte peak areas. If this should occur, perform the
procedure outlined in Section 5.2, “Small or increasing Analyte Peak Areas.”
5.1 Electrolytically Regenerated Suppressor Operational Status Displays Alarm State
ERS or SRS Alarm:
Alarm state indicates that there is high resistance or an open circuit in the suppressor device
typically in the regenerant channel or there is an issue with connectivity of the suppressor to a
power supply.
High resistance or an open circuit occurs when:
1.
The suppressor is not connected to the power supply or has a defective cable. In this
state the only indicator from the system is a high voltage alarm. In this state no current
can be applied to the suppressor.
2.
The suppressor is operated with higher currents than the recommended currents.
Refer to the current settings section to determine the correct current and lower the
current.
3.
The suppressor is exposed to contaminants such as iron, other metals or organics.
Refer to the appropriate cleanup procedure and implement the recommended cleanup.
In cases when the contamination is continuous, a routine cleanup step on a weekly
basis is recommended as part of preventive maintenance.
!
NOTE
If the above problem is encountered on a routine basis particularly with samples
containing high levels of metals or solvents, the chemical mode of operation is
recommended. The Dionex CRS 500 is recommended for the chemical mode of operation;
the Dionex ERS 500 cannot be operated in the chemical mode.
Refer to Appendix C for alarm state information on discontinued models.
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 60 of 84
5 – Troubleshooting Guide
5.2 Small or Increasing Analyte Peak Areas
This problem is caused by running eluent through the Dionex ERS 500, Dionex ERS 500e, or
Dionex AERS 500 Carbonate with the power off while using the AutoSuppression Recycle
Mode or the AutoSuppression External Water Mode. It may also be caused by application to too
little current to the suppressor for an extended period of time. A regeneration protocol is needed
to ensure good operation.
!
NOTE
5.2.1
The Dionex AERS 500 Carbonate suppressor has different electrical current requirements
than the Dionex AERS 500 and Dionex ERS 500e, suppressors. If an AERS_Carbonate
mode is not available the current applied to a Dionex AERS 500 Carbonate should be
adjusted by a factor of 1.30 compared to a standard AERS 500 suppressor. Chromeleon 7.2
SR3 MUa and later versions have an option for selecting an AERS_Carbonate suppressor
in the Instrument Method Wizard and Editor dialog box. Selecting this suppressor from the
drop-down list will automatically apply the recommended current setting for Dionex AERS
500 Carbonate suppressors.
Suppressor Chemical Regeneration Steps
For fast regeneration it is recommended to follow the steps below for Chemical Regeneration of
the suppressor.
A. Disconnect the eluent line from the analytical column attached to the ELUENT IN port
of the Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500 Carbonate at the
analytical column end of the line. Direct this line to a separate waste beaker.
B. Disconnect the eluent line from the ELUENT OUT port of the suppressor.
C. For the Dionex AERS 500, Dionex AERS 500e or Dionex AERS 500 Carbonate:
Using a plastic syringe push approximately 3 mL of 200 mN H2SO4 through the
ELUENT OUT port and approximately 5 mL of 200 mN H2SO4 through the REGEN
IN port. Flush with 3 mL degassed, deionized water through the ELUENT OUT port
and 5 mL degassed, deionized water through the REGEN IN port.
D. For the Dionex CERS 500 or Dionex CERS 500e:
Using a plastic syringe slowly push approximately 3 mL of freshly prepared 200 mN
NaOH (made up using degassed, deionized water) through the ELUENT OUT port and
approximately 5 mL of 200 mN NaOH through the REGEN IN port. Flush with 3 mL
degassed, deionized water through the ELUENT OUT port and 5 mL degassed,
deionized water through the REGEN IN port.
E. Reconnect the eluent line from the ELUENT IN port of the suppressor to the analytical
column and the eluent line from the eluent out port of the suppressor to the
conductivity detector cell.
F. If you are in the Auto Suppression Recycle mode of operation, begin pumping eluent
and immediately turn on the power. DO NOT LET THE ELUENT FLOW THROUGH
THE SUPPRESSOR FOR MORE THAN A FEW MINUTES WITHOUT TURNING
ON THE POWER.
If you are in the Auto Suppression External water mode of operation, establish water
flow through the regenerant chambers, begin pumping eluent and immediately turn on
the power. DO NOT LET THE ELUENT FLOW THROUGH THE SUPPRESSOR
FOR MORE THAN A FEW MINUTES WITHOUT TURNING ON THE POWER.
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 61 of 84
5 – Troubleshooting Guide
5.2.2
Suppressor Electrolytic Regeneration Steps
A. The Dionex ERS 500, Dionex ERS 500e and Dionex AERS 500 Carbonate are unique
that they can be regenerated electrolytically. Although this is slower than Chemical
Regeneration, it does not require the suppressor to be disconnected or the use of any
reagents.
B. Turn on the system with the normal application settings, if using gradient elution, set
the eluent concentration to the maximum value usually seen in the gradient.
C. Set the current of the suppressor to 60% higher than the optimal current. e.g., if the
optimal current is 50 mA, set the current to 80 mA. This is the maximum suppressor
current reported by Chromeleon in the Instrument Program settings.
D. Leave the system running with the suppressor set to the higher current for a minimum
of 3 hours, up to 6 hours.
E. Set the current back to the optimal current.
!
CAUTION
5.2.3
Do not exceed the maximum allowed current for a suppressor:
Dionex ERS 500 (4 mm) and Dionex ERS 500e (4 mm): 500 mA
Dionex ERS 500 (2 mm) and Dionex ERS 500e (2 mm): 100 mA
Dionex AERS 500 Carbonate (4 mm): 125 mA
Dionex AERS 500 Carbonate (2 mm): 30 mA.
Full or Extended Regeneration:
Depending on the extent of capacity loss of the suppressor, the regeneration procedure as
outlined above may be inadequate to fully regenerate the suppressor. In order to fully regenerate
the suppressor an extended rinse (for at least two hours) with 200 mN sulfuric acid (for Dionex
AERS 500, Dionex AERS 500e or Dionex AERS 500 Carbonate) or 200 mN NaOH (for Dionex
CERS 500 or Dionex CERS 500e) at the application flow rate is needed. Pump the acid or base
(as the case may be) through the eluent out port of the suppressor, with a line connecting the
eluent in port to the regen in port. Divert a line from the Regen out port to waste. A standalone
pump or a trap column/suppressor clean-up kit (Item # 059659) could be used for the above
regeneration. Another option is to pursue the regeneration as outlined in steps 5.2.1. C or D
above and then allow the acid or base to soak in the suppressor overnight. In the morning repeat
the regeneration as outlined in steps 4.2.1. C or D and then displace the acid or base with DI
water.
If the correct peak areas are not observed following two injections of a standard test solution,
contact the nearest Thermo Fisher Scientific Regional Office (see Thermo Fisher Scientific
Worldwide Offices on the Reference Library CD-ROM).
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 62 of 84
5 – Troubleshooting Guide
Figure 14
Trend plot showing Increasing Peak Areas after a suppressor was operated
with the power off for 40 minutes. Blue trace shows recovery without
following the Regeneration steps, Purple trace show recovery during
Suppressor Electrolytic Regeneration process.
120%
Recovery without Regeneration
Recovery with 60% extra current
Suppressor Peak Area Recovery (% phosphate)
100%
80%
60%
40%
20%
0%
0.0
1.0
2.0
3.0
4.0
5.0
6.0
Time (hours) after suppressor shutdown
7.0
8.0
9.0
NOTE
The sulfuric acid in the above steps could be replaced with a non-oxidizing strong acid
such as Methanesulfonic acid (MSA) which is recommended when pursuing MS
applications.
!
Do not use the Analytical pump for regeneration purpose as this would contaminate the
pump
!
NOTE
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 63 of 84
5 – Troubleshooting Guide
5.3 High Background Conductivity
A. Check the Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500 Carbonate current
settings.
Refer to the suppressor current settings section.
B. Check for eluent flow out of the suppressor ELUENT OUT port.
!
1.
If there is no flow out of the suppressor ELUENT OUT port, make sure that eluent
is entering the suppressor at the ELUENT IN port. If there is no flow at this point,
trace the eluent flow path backward through the system to find and remove the
blockage.
2.
If there is flow into the suppressor but not out, and there are no visible leaks from
the rear seam of the suppressor, a break in the suppressor seal is probably allowing
eluent to leak into the regenerant chambers. If this is the case, then the suppressor
should be replaced. The suppressor is sealed during manufacture; attempting to
open it will destroy it.
Do NOT attempt to disassemble the Dionex ERS 500, Dionex ERS 500e or Dionex AERS
500 Carbonate.
SAFETY
3.
If there is flow from the ELUENT OUT port, but no eluent suppression, the
membrane may have been contaminated. Try to restore system performance by
cleaning the membrane (see Section 6, “Electrolytically Regenerated Suppressor
Cleanup”).
C. Remake the eluent to be sure that the concentration is correct. Be sure that chemicals of
the required purity were used to make the eluent (see Section 3.1, “Chemical Purity
Requirements”). If the eluent concentration is high, the suppressor may not be set up to
suppress the high concentration resulting in high background conductivity. Refer to
Tables 13–17 in Appendix A, “Matching the Current Setting to the Eluent
Concentration and Flow Rate,” for Dionex AERS 500, Dionex AERS 500e and Dionex
AERS 500 Carbonate suppressors and the Tables 18–20 in Appendix A for the Dionex
CERS 500 and Dionex CERS 500e.
D. Contact the nearest Thermo Fisher Scientific Regional Office (see, “Thermo Fisher
Scientific Worldwide Offices”) if you cannot solve the problem on your own.
5.4 Drifting Baseline
If the baseline drifts steadily upward, increase the current setting by 5-10% to reduce the
background conductivity. As the background conductivity decreases, the baseline drift should
decrease.
5.5 Noisy Baseline
If the baseline is noisy (> 3 nS with hydroxide or MSA eluents, > 10 nS with borate eluents, > 7
nS with carbonate or carbonate/bicarbonate eluents and the Dionex AERS 500 or Dionex AERS
500e, > 2 nS with carbonate or carbonate/bicarbonate eluents and the Dionex AERS 500
Carbonate), it could be caused by trapped air bubbles in the cell or tubing. Burp or release the
trapped bubbles by gently tapping on the cell while the fittings are slightly loosened or bleeding
the tubing. Below is an example:
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 64 of 84
5 – Troubleshooting Guide
Figure 19
Effect of Air Bubbles on Baseline
Dionex AERS 500 (4 mm), Recycle Eluent Mode
In the above figure, a bubble is released from ELUENT IN line by loosening and tightening the
fitting at 56.6 minutes into the run.
Noise:
Thermo Scientific
031956-11
~2.5 nS
before
< 0.5 nS
after
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 65 of 84
5 – Troubleshooting Guide
5.6 Decreased Sensitivity
A. Check for leaks throughout the system. If a fitting is leaking, tighten it carefully until
the leak stops. Do not over tighten. If the Dionex ERS 500, Dionex ERS 500e or
Dionex AERS 500 Carbonate is observed to be leaking from the center or bottom
seam, see Section 5.8, “Liquid Leaks.” If you cannot cure the problem yourself, call the
nearest Thermo Fisher Scientific Regional Office (see, “Thermo Fisher Scientific
Regional Offices”) for assistance.
B. Ensure that the injection valve is operating correctly. Refer to the valve manuals that
accompany the chromatography module for troubleshooting assistance. For slider
valves, be sure to check the slider port faces for damage.
C. Pursue regeneration of the suppressor as outlined in Section 5.2
D. If sensitivity remains low, clean the suppressor membrane (see Section 6, “Dionex
ERS 500, Dionex ERS 500e and Dionex AERS 500 Carbonate Suppressor Cleanup”).
E. Check the backpressure coils. Verify that they are not exceeding 40 psi in the current
plumbing configuration and flow rate.
F. Replace the suppressor if cleaning the suppressor membrane does not restore
sensitivity.
G. Contact the nearest Thermo Fisher Scientific Regional Office (see, “Thermo Fisher
Scientific Worldwide Offices”) if you cannot solve the problem on your own.
5.7 System Back Pressure Increases Over Time
If the increased back pressure does not affect system performance, no maintenance is necessary.
A. Check the inlet frits on the guard and analytical column and replace them if necessary.
The most common cause of increasing system back pressure is a contaminated frit in
the analytical or guard column inlet end fitting. The complete instructions for replacing
column bed support assemblies are in Document No. 032285. Recheck the system back
pressure. If it remains high, go on to the next step.
B. Check the backpressure coils. If removing the backpressure coils lowers the pressure
by more than 40 psi, replace the coils or remove the blockage causing the increased
pressure. Backpressure over 450 psi after the suppressor can cause irreversible damage.
C. Find and eliminate any system blockage. Bypass the Dionex ERS 500, Dionex ERS
500e or Dionex AERS 500 Carbonate by coupling the lines attached to the ELUENT
IN and ELUENT OUT ports. If the back pressure decreases by less than 150 psi with
the suppressor out of line, a blockage in the system rather than in the suppressor is
causing the high pressure.
D. Remove a blockage from the suppressor by reversing the eluent flow. If the back
pressure decreases by more than 150 psi with the suppressor out of line, the high
pressure may be caused by a blockage in the suppressor. Reverse the direction of flow
of both the eluent and the external water through the suppressor. After the pressure
drops, allow eluent, or eluent and regenerant, to flow to waste for several minutes after
the pressure drops. Perform step A of Section 3.2, “Startup” and reinstall the
suppressor in the appropriate configuration.
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 66 of 84
5 – Troubleshooting Guide
E. Clean the suppressor membranes if reversing the flow through the suppressor does not
decrease the pressure. (See Section 6, “Dionex ERS 500, Dionex ERS 500e and
Dionex AERS 500 Carbonate Suppressor Cleanup”).
F. Replace the suppressor if cleaning the suppressor membrane does not reduce the
pressure.
G. Contact the nearest Thermo Fisher Scientific Regional Office (see, “Thermo Fisher
Scientific Regional Offices”) if you cannot solve the problem on your own.
5.8 Liquid Leaks
A. If there is leakage from the side seam of the Dionex ERS 500, Dionex ERS 500e or
Dionex AERS 500 Carbonate, check for leakage from one of the four ports of the
Dionex ERS 500 into the housing and check the back pressure after the suppressor.
B. If there is liquid coming out of any of the four ports of the Dionex ERS 500, Dionex
ERS 500e or Dionex AERS 500 Carbonate, carefully tighten the fittings in the leaking
port – the fittings do not need to be more than ¼-turn past finger tight. If tightening the
fittings does not stop the leak, replace the ferrules and bolts on the tubing.
C. If the system back pressure is greater than 450 psi, the leaks are likely caused by
excessive back pressure downstream from the suppressor. Find and eliminate the
source of the pressure. The suppressor will usually recover from momentary
overpressure conditions if allowed to stand approximately 20 minutes with the
membranes fully hydrated. See the Caution Note in Section 3.2, “Start-Up” If the
suppressor continues to leak when operated within the proper back pressure range, it
must be replaced.
5.9 Poor or unstable recovery of certain peaks.
If one or two peaks are experiencing poor or unstable recoveries while the other peaks are
stable, it could be that the current to the suppressor is set too high. Recalculate the correct
current setting (see Section 2.6, or use the “signal parameters” tool in Chromeleon). Do not
exceed the recommended current setting unless necessary to stabilize a drifting baseline.
Alternatively the system may be contaminated with a transition metal that is binding to the
analyte of interest (See Section 6.1, “Metal Contaminants or Precipitates”).
5.10 Peaks and spikes in the absence of an injection
A. Excessive current applied to the suppressor: Recalculate the optimum current and
apply; never apply more than 10% above the optimum current except when executing
the Electrolytic Regeneration Steps. When operating with gradient eluents, apply the
minimum current setting for the maximum eluent concentration at the eluent flow rate.
During a low flow method (stand-by mode), ensure that the current is lowered to the
optimal value based on the reduced flow rate and/or eluent concentration.
B. Precipitation on the suppressor membrane or screens (calcium, magnesium and other
metals): Follow the Metal Contaminants or Precipitates procedure from Section 6.1. To
prevent contaminants from reaching the suppressor, a CP1 cation polisher column
(Item # 064930) can be used during anion analysis to strip cationic contaminants from
the sample. Refer to the CP1 Operator’s Manual for detailed instructions.
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 67 of 84
5 – Troubleshooting Guide
C. Outgassing or trapped bubbles in the suppressor regenerant chambers: Ensure that the
external water (if used) is degassed before use; do not pressurize the external water
with air, use nitrogen or helium. Eliminate causes of excessive backpressure between
the cell outlet and the regenerant inlet if recycled eluent mode is used.
D. Excess suppressor temperature: Ensure liquid entering the suppressor is at or less than
35°C. Ensure the suppressor is operated in an environment that does not exceed 35°C
during operation. Refer to Section 3.3.3 Installation in thermal chamber. 20°C is the
optimum temperature for Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500
Carbonate operation.
E. Large changes to the flow rate or applied current of the suppressor: When changing
eluent concentration or flow rate, recalculate the optimum current using the attached
calculator; do not allow the suppressor to operate for more than 5 minutes with
excessive or insufficient current. If the current is changed, allow the suppressor a few
hours to reestablish baseline stability. Ensure that shut-down, stand-by and start-up
methods are applying the correct suppressor current.
F. Operating the suppressor with power but no eluent or regenerant flow: Operation of the
suppressor without flow may irreversibly damage the suppressor, depending on amount
of applied current and duration. Ensure that the regenerant lines are connected and flow
is established when powering on the suppressor at all times. Ensure that during external
water operation that the suppressor is never operated without regenerant flow. When
operating properly, bubbles interspersed with liquid exiting the regenerant chamber
indicates good flow. Replace the suppressor if operation without flow is known to have
occurred. Confirm that the minimum pressure limit on the pump is set to a non-zero
value to ensure that the system turns off in the event of a leak.
G. Incorrect current setting during shutdown, stand-by or startup method: Shut-down
methods should shut the pump flow off at the same time as the suppressor current.
Stand-by methods should reset the suppressor current to the optimum level for the
reduced flow rate and/or concentration. Do not reduce the flow rate on RFIC-EG
systems; this will lower the backpressure on the degasser below 2,000 psi and cause
out-gassing. Stand-by methods for RFIC-EG systems should reduce eluent
concentration to preserve Dionex EGC ion count; reset the suppressor current
accordingly. Do not turn the Dionex EGC concentration to zero with flow; use a low
setting. Consider using the smart-shutdown and smart-startup feature of Chromeleon
instead of standby conditions and configure the system for smart-startup two hours
before use is anticipated so the system is equilibrated and ready to run. Consider using
RFIC-ER; no shut-down, stand-by or start-up procedure is needed with an Always On,
Always Ready system. Start-up settings should turn on the pump flow and suppressor
current simultaneously.
5.11 Low Sample Response (Neutralization Mode Only)
A. If the Dionex ERS 500e is observed to be leaking, see Section 5.8. “Liquid Leaks.”
B. Ensure the Sample Injection Valve and Recycle Valve are operating correctly. Refer to
the valve manuals that accompany the valves. Be sure to check the port faces for
damage.
C. If the neutralization requires excessive recycling, clean the suppressor membranes (see
Section 6, Dionex ERS 500, Dionex ERS 500e and Dionex AERS 500 Carbonate
Cleanup).
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 68 of 84
5 – Troubleshooting Guide
D. If cleaning the suppressor membranes does not restore neutralization efficiency, the
suppressor may need to be replaced.
E. If you cannot solve the problem on your own, contact the Thermo Scientific Regional
Office nearest you.
5.12 Low Neutralization Capacity (Neutralization Mode Only)
For the Dionex AERS 500e this problem is caused when the ion exchange sites in the
suppressor are converted from the hydronium form to the salt form. They must be converted
back to the hydronium form for efficient operation.
For the Dionex CERS 500e this problem is caused when the ion exchange sites in the suppressor
are converted from the hydroxide form to a salt form. They must be converted back to the
hydroxide form for efficient operation.
Pursue regeneration of the suppressor as outlined in Section 5.2. If capacity remains low, clean
the suppressor membrane (see Section 6, “Dionex ERS 500, Dionex ERS 500e and Dionex
AERS 500 Carbonate Suppressor Cleanup”).
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 69 of 84
6 – Dionex Suppressor Cleanup
6. Dionex ERS 500, Dionex ERS 500e and
Dionex AERS 500 Carbonate Suppressor
Cleanup
This section describes routine cleanup procedures for the Dionex Electrolytically Regenerated
Suppressors (Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500 Carbonate) in the case
of contamination. Consult the Troubleshooting Guide (see Section 5, “Troubleshooting Guide”)
to first determine that the system is operating properly. If the suppressor is determined to be the
source of higher than normal back pressure, higher than anticipated conductivity, decreased
suppression capacity or decreased sensitivity, cleaning the membrane may restore the
performance of the system. Use the following procedures to clean the membrane.
6.1 Metal Contaminants or Precipitates
!
NOTE
The suppressor voltage is a good indicator of the resistance across the suppressor. Higher
resistance may indicate contamination of the suppressor. For more information regarding
monitoring the voltage, see Document No. 031841-02 “Removal of Iron Contamination
from Electrolytic Suppressors.”
A. Turn off the Dionex ERS or Dionex SRS Control unit and system pump.
B. Disconnect the analytical (and guard) column(s) from the injection valve and the
suppressor. Refer to the specific analytical column Product Manual for column cleanup
procedures.
C. If you are running in the AutoSuppression External Water Mode, turn off the external
water and disconnect the external water line from the suppressor REGEN IN port.
D. Disconnect the liquid line from the suppressor ELUENT OUT port to the cell at the
cell fitting and reconnect it to the REGEN IN port.
E. If iron is present then connect a temporary line from the priming block or the lowpressure tee on the isocratic or gradient pump to a container with a solution of 0.2 M
oxalic acid. For 4 mm systems pump this solution through the suppressor at 1 – 2
mL/min for 30 minutes. For 2 mm systems pump this solution through the suppressor
at 0.25–0.50 mL/min for 30 minutes. Proceed to step G.
F. If iron is not present then proceed to step G.
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 70 of 84
6 – Dionex Suppressor Cleanup
!
NOTE
Bypassing internal pump manifolds when temporarily pumping high concentration
cleaning solutions significantly reduces the time required to re-equilibrate the system to
low concentration eluents.
G. Pursue an extended regeneration as outlined in Section 5.2.3
H. Reinstall the analytical (and guard) column(s). Begin pumping eluent through the
system at the flow rate required for your analysis and equilibrate the system and
resume normal operation.
!
NOTE
Chromeleon 6.5 and later includes a feature to Trend various parameters. Trend plotting
the suppressor voltage will reveal if there is a build-up of metal contaminants or
precipitates in the suppressor. A slow but steady increase in voltage indicates such a
contamination build-up
6.2 Organic Contaminants
A. Turn off the ERS Control or SRS Control unit and system pump.
B. Disconnect the analytical (and guard) column(s) from the injection valve and the
Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500 Carbonate. Refer to the
specific analytical column Product Manual for column cleanup procedures.
C. If you are running in the AutoSuppression External Water Mode, turn off the external
water and disconnect the external water line from the suppressor REGEN IN port.
D. Disconnect the liquid line from the suppressor ELUENT OUT port to the cell at the
cell fitting and reconnect it to the REGEN IN port.
E. Connect a temporary line from the priming block or the low-pressure tee on the
isocratic or gradient pump to a container with a solution of freshly prepared 10% 1.0 N
methane sulfonic acid or sulfuric acid and 90% acetonitrile or methanol.
Acid/acetonitrile solutions are not stable during long term storage so this cleanup
solution must be made immediately before each column cleanup. Alternatively, it can
be proportioned from 1 bottle containing 1.0 N acid and another bottle containing
100% acetonitrile. For 4 mm systems pump this solution through the suppressor at 1 –
2 mL/min for 30 minutes. For 2 mm systems, pump this solution through the
suppressor at 0.25–0.50 mL/min for 30 minutes.
!
NOTE
Bypassing internal pump manifolds when temporarily pumping high concentration
cleaning solutions significantly reduces the time required to re-equilibrate the system to
low concentration eluents.
F. Flush the suppressor with deionized water for 10 minutes.
G. Reinstall the analytical (and guard) column(s). Begin pumping eluent through the
system at the flow rate required for your analysis and equilibrate the system and
resume normal operation.
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 71 of 84
Appendix A – Current Settings
Appendix A – Current Settings
A.1. Optimum Current Settings for Common Eluents; Dionex AERS 500 (4 and 2 mm)
and Dionex AERS 500e (4 and 2 mm)
The Dionex Anion Electrolytically Regenerated Suppressor (Dionex AERS 500 and Dionex
ERS 500e) uses water as the regenerant and has the ability to provide continuous suppression.
Table 13 and Table 17 list the eluent concentrations and flow rates of commonly used mobile
phases used in anion separations and the optimum / recommended current level to suppress the
eluent. The operation of the Dionex AERS 500 and Dionex ERS 500e requires a constant flow
of water over the electrodes in a direction that is countercurrent to the flow of the eluent.
In the AutoSuppression Recycle Mode, the eluent leaving the conductivity cell is recycled
through the regenerant chambers as the water supply. This eliminates the need for an external
regenerant water supply and delivery system. When the Dionex AERS 500 is operating in this
mode, the amount of water flowing through the regenerant chambers is limited to the eluent
flow rate. The AutoSuppression Recycle Mode cannot be used with eluents containing any
organic solvents.
The AutoSuppression External Water Mode requires an external source of deionized water for
the regenerant chambers. When the Dionex AERS 500e is operating in this mode, the amount of
water flowing through the regenerant chambers is independent of the eluent flow rate. Because
of this, higher regenerant flow rates are achievable, see Table 15. The AutoSuppression External
Water Mode is the mode used if organic solvents (up to 40%) are present in the eluent. It
eliminates the potential for buildup of contaminating ions resulting from the oxidation of
solvents.
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 72 of 84
Appendix A – Current Settings
Table 13
Optimum Current Settings for the Dionex AERS 500 and Dionex ERS 500e in
the AutoSuppression Recycle and External Water Modes
Eluent Flow Rate (mL/min)
(2 mm)
(4 mm)
0.5
2.0
0.25
1.0
0.38
1.5
0.3
1.2
0.5(1)
1.0
0.3
1.2
0.25
1.0
0.25
1.0
0.25
1.0
0.5
2.0
0.38
1.5
0.38
1.5
0.3
1.2
0.5(1)
0.25
1.0
0.38
1.5
0.25
1.0
0.5
2.0
0.25
1.0
0.25
1.0
0.25
1.0
0.25
1.0
0.25
1.0
0.25
1.0
0.35
0.30
Optimum Current (mA)
(2 mm)
(4 mm)
7
27
12
45
6
22
6
24
21
42
8
31
7
25
50
198
8
30
48
190
29
112
57
223
29
113
50
22
87
52
204
10
38
50
198
15
57
25
97
13
50
28
112
22
87
34
136
13
41
Column
Eluent
AS4A-SC
AS9-HC
AS12A
AS14
AS14A
AS22
AS23
AS10
AS11
AS21
AS24
1.8 mM CO32- / 1.7 mM HCO39.0 mM CO322.7 mM CO32- / 0.3 mM HCO33.5 mM CO32- / 1.0 mM HCO38.0 mM CO32- / 1.0 mM HCO34.5 mM CO32- / 1.4 mM HCO34.5 mM CO32- / 0.8 mM HCO380 mM OH–
12 mM OH–
38.3 mM OH–
30 mM OH–
60 mM OH–
38 mM OH–
40 mM OH–
35 mM OH–
55 mM OH–
15 mM OH–
40 mM OH–
23 mM OH–
39 mM OH–
20 mM OH–
45 mM OH–
35 mM OH–
55 mM OH–
15 mM OH–
55 mM OH–
AS25
36 mM OH–
0.25
1.0
23
92
AS26
–
0.30
1.2
41
164
AS11-HC
AS15
AS16
AS17-C
AS18
AS19
AS20
(1)
55 mM OH
3 mm format
The Dionex AERS 500 and Dionex AERS 500e can be used with legacy systems that only
provide discrete (50, 100, 300 and 500 mA) current settings, see Error! Reference source not
ound. and Error! Reference source not found.; note that some compromise in performance
and product life-expectancy may be observed. For optimum performance it is recommended to
use a Dionex RFC-10 or Dionex RFC-30 power supply to provide fine current control with the
optimum setting.
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 73 of 84
Appendix A – Current Settings
Table 14
Column
Recommended Current Settings for the Dionex AERS 500 and
Dionex AERS 500e in the AutoSuppression Recycle and External Water
Modes with legacy discrete power supplies
Eluent
1.8 mM CO32- / 1.7 mM
HCO3AS9-HC
9.0 mM CO32AS12A
2.7 mM CO32- / 0.3 mM
HCO3AS14
3.5 mM CO32- / 1.0 mM
HCO3AS14A
8.0 mM CO32- / 1.0 mM
HCO3AS22
4.5 mM CO32- / 1.4 mM
HCO3AS23
4.5 mM CO32- / 0.8 mM
HCO3AS10
80 mM OH–
AS11
12 mM OH–
38.3 mM OH–
AS11-HC
30 mM OH–
60 mM OH–
AS15
38 mM OH–
40 mM OH–
AS16
35 mM OH–
55 mM OH–
AS17-C
15 mM OH–
40 mM OH–
AS18
23 mM OH–
39 mM OH–
AS19
20 mM OH–
45 mM OH–
AS20
35 mM OH–
55 mM OH–
AS21
15 mM OH–
AS24
55 mM OH–
AS25
36 mM OH–
AS26
55 mM OH–
(1)
3 mm format
AS4A-SC
Thermo Scientific
031956-11
Eluent Flow Rate
(mL/min)
(2 mm)
(4 mm)
0.5
2.0
Recommended Current
(mA)(2 mm)
(4 mm)
50
50
0.25
0.38
1.0
1.5
50
50
50
50
0.3
1.2
50
50
0.5(1)
1.0
50
50
0.3
1.2
50
50
0.25
1.0
50
50
0.25
0.25
0.5
0.38
0.38
0.3
0.5(1)
0.25
0.38
0.25
0.5
0.25
0.25
0.25
0.25
0.25
0.25
0.35
0.30
0.25
0.30
1.0
1.0
2.0
1.5
1.5
1.2
50
50
50
50
100
50
50
50
100
50
50
50
50
50
50
50
50
50
50
50
50
300
50
300
300
300
300
1.0
1.5
1.0
2.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.2
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
100
300
50
300
100
100
50
300
100
300
100
300
Page 74 of 84
Appendix A – Current Settings
Table 15
External Water Flow Rates for Dionex AERS 500 and
Dionex AERS 500e (4 and 2 mm)
Regenerant Flow Rate
(mL/min)(1)
1 – 5 mL/min
Dionex AERS 500 (4 mm)
equal to the eluent flow rate recommended
0.25 – 2 mL/min
Dionex AERS 500 (2 mm)
equal to the eluent flow rate recommended
2 – 10 mL/min
Dionex AERS 500e (4 mm)
equal to 2x eluent flow rate recommended
0.5 – 4 mL/min
Dionex AERS 500e (2 mm)
equal to 2x eluent flow rate recommended
(1)
Measured with power ON using a graduated cylinder
Dionex AERS 500 Format
Refer to Section A.3, of this manual for recommended Current Switch settings required for the
chromatographic conditions required in the specific application being performed.
A.2. Optimum Current Settings for Common Eluents; Dionex AERS 500 Carbonate (4
and 2 mm)
The Dionex Anion Electrolytically Regenerated Suppressor for Carbonate Eluents (Dionex
AERS 500 Carbonate) uses water as the regenerant and has the ability to provide continuous
suppression. Table 13 to Table 17 list the eluent concentrations and flow rates of commonly
used mobile phases used in anion separations and the optimum / recommended current level to
suppress the eluent. The operation of the Dionex AERS 500 Carbonate requires a constant flow
of water over the electrodes in a direction that is countercurrent to the flow of the eluent.
In the AutoSuppression Recycle Mode, the eluent leaving the conductivity cell is recycled
through the regenerant chambers as the water supply. This eliminates the need for an external
regenerant water supply and delivery system. When the Dionex AERS 500 Carbonate is
operating in this mode, the amount of water flowing through the regenerant chambers is limited
to the eluent flow rate. The AutoSuppression Recycle Mode cannot be used with eluents
containing any organic solvents.
The AutoSuppression External Water Mode requires an external source of deionized water for
the regenerant chambers. When the Dionex AERS 500 Carbonate is operating in this mode, the
amount of water flowing through the regenerant chambers is independent of the eluent flow rate.
Because of this, higher regenerant flow rates are achievable, see Table 17. The AutoSuppression
External Water Mode is the mode used if organic solvents (up to 40%) are present in the eluent.
It eliminates the potential for buildup of contaminating ions resulting from the oxidation of
solvents.
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 75 of 84
Appendix A – Current Settings
Table 16
Column
AS4A-SC
AS9-HC
AS12A
AS14
AS14A
AS22
AS22-Fast
AS23
(1)
3 mm format
Optimum Current Settings for the Dionex AERS 500 Carbonate in the
AutoSuppression Recycle and External Water Modes
Eluent
1.8 mM CO32- / 1.7 mM HCO39.0 mM CO322.7 mM CO32- / 0.3 mM HCO33.5 mM CO32- / 1.0 mM HCO38.0 mM CO32- / 1.0 mM HCO34.5 mM CO32- / 1.4 mM HCO34.5 mM CO32- / 1.4 mM HCO34.5 mM CO32- / 0.8 mM HCO3-
Eluent Flow Rate (mL/min)
(2 mm)
(4 mm)
0.5
2.0
0.25
1.0
0.38
1.5
0.3
1.2
0.5(1)
1.0
0.3
1.2
0.5
2.0
0.25
1.0
Optimum Current (mA)
(2 mm)
(4 mm)
9
34
15
58
7
28
8
31
28
55
11
41
17
67
8
32
The Dionex AERS 500 cannot be used with legacy systems that only provide discrete (50, 100,
300 and 500 mA) current settings.
Table 17
External Water Flow Rates for Dionex AERS 500 Carbonate (4 and 2 mm)
Dionex AERS 300
Format
Regenerant Flow Rate
(mL/min)(1)
4 mm
3 – 5 mL/min; at least 2x the eluent flow rate
2 mm
1 – 2 mL/min; at least 2x the eluent flow rate
(1)
Measured with power ON using a graduated cylinder
A.3. Optimum Current Settings for Common Eluents; Dionex CERS 500 (4 mm and
2 mm) and Dionex CERS 500e (4 mm and 2 mm)
The Dionex Cation Electrolytically Regenerated Suppressor (Dionex CERS 500 and Dionex
CERS 500e) uses water as the regenerant and has the ability to provide continuous suppression.
Table 18 - 20 list the eluent concentrations and flow rates of commonly used mobile phases
used in cation separations and the optimum / recommended current level to suppress the eluent.
The operation of the Dionex CERS 500 and Dionex CERS 500e requires a constant flow of
water over the electrodes in a direction that is countercurrent to the flow of the eluent.
In the AutoSuppression Recycle Mode, the eluent leaving the conductivity cell is recycled
through the regenerant chambers as the water supply. This eliminates the need for an external
regenerant water supply and delivery system. When the Dionex CERS 500 is operating in this
mode, the amount of water flowing through the regenerant chambers is limited to the eluent
flow rate. The AutoSuppression Recycle Mode cannot be used with eluents containing any
organic solvents.
The AutoSuppression External Water Mode requires an external source of deionized water for
the regenerant chambers. When the Dionex CERS 500 is operating in this mode, the amount of
water flowing through the regenerant chambers is independent of the eluent flow rate. Because
of this, higher regenerant flow rates are achievable, see Table 20. Higher regenerant flow rates
translate in improved signal-to-noise ratios compared to the AutoSuppression Recycle Mode.
The AutoSuppression External Water Mode is the mode used if organic solvents (up to 40%) are
present in the eluent. It eliminates the potential for buildup of contaminating ions resulting from
the oxidation of solvents.
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 76 of 84
Appendix A – Current Settings
Table 18
Column
CS12A
Eluent
Optimum Current Settings for the Dionex CERS 500 and Dionex CERS 500e in
the AutoSuppression Recycle and External Water Modes
Eluent Flow Rate
(mL/min)
(2 mm)
(4 mm)
0.25
1.0
0.25
1.0
0.5(1)
0.5(1)
0.25
1.0
0.3
1.2
0.3
1.2
0.36(1)
1.0(3)
0.36(1)
1.0(3)
0.25
1.0
0.25
1.0
0.25
0.25
1.0
0.3
1.2
20 mM MSA
33 mM MSA
CS12A20 mM MSA
5μm
33 mM MSA
CS14
10 mM MSA
CS15
10 mM H2SO4/9% AcN(2)
14 mM H2SO4
CS16
30 mM MSA
48 mM MSA
CS17
6 mM MSA
40 mM MSA
CS18
5 mM MSA
CS19
8 mM MSA
60 mM
(1)
3 mm format
(2)
Compatible with External Water Mode only
(3)
5 mm format
Optimum Current (mA)
(2 mm)
(4 mm)
15
25
30
49
8
18
25
32
51
5
30
4
6
53
59
98
30
71
99
89
142
18
118
24
212
The Dionex CERS 500 and Dionex CERS 500e can be used with legacy systems that only
provide discrete (50, 100, 300 and 500 mA) current settings, see Error! Reference source not
ound. and Error! Reference source not found. note that some compromise in performance
may be observed. For optimum performance it is recommended to use a Dionex RFC-10 or
Dionex RFC-30 power supply to provide graduated current control with the optimum setting.
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 77 of 84
Appendix A – Current Settings
Table 19
Column
Recommended Current Settings for the Dionex CERS 500 and Dionex CERS
500e in the AutoSuppression Recycle and External Water Modes with
legacy discrete power supplies
Eluent Flow Rate (mL/min)
(2 mm)
(4 mm)
0.25
1.0
0.25
1.0
0.5(1)
0.5(1)
0.25
1.0
0.3
1.2
0.3
1.2
0.36(1)
1.0(3)
0.36(1)
1.0(3)
0.25
1.0
0.25
1.0
0.25
0.25
1.0
0.3
1.2
Eluent
CS12A
20 mM MSA
33 mM MSA
CS12A-5μm 20 mM MSA
33 mM MSA
CS14
10 mM MSA
CS15
10 mM H2SO4/9% AcN(2)
14 mM H2SO4
CS16
30 mM MSA
48 mM MSA
CS17
6 mM MSA
40 mM MSA
CS18
5 mM MSA
CS19
8 mM MSA
60 mM
(1)
3 mm format
(2)
Compatible with External Water Mode only
(3)
5 mm format
Table 20
Recommended Current (mA)
(2 mm)
(4 mm)
50
100
50
100
50
50
50
50
50
100
50
100
50
100
100
300
50
50
50
300
50
50
50
100
300
External Water Flow Rates for Dionex CERS 500 and
Dionex CERS 500e (4 mm and 2 mm)
Regenerant Flow Rate
(mL/min)(1)
1 – 5 mL/min
Dionex CERS 500 (4 mm)
equal to the eluent flow rate
0.25 – 2 mL/min
Dionex CERS 500 (2 mm)
equal to the eluent flow rate
2 – 10 mL/min
Dionex CERS 500e (4 mm)
equal to 2x eluent flow rate
0.5 – 4 mL/min
Dionex CERS 500e (2 mm)
equal to 2x eluent flow rate
(1)
Measured with power ON using a graduated cylinder
Dionex CERS 500 Format
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 78 of 84
Appendix A – Current Settings
A.2
Current Settings for Older Detectors
On CD20 and ED40 detectors in Dionex DX-500 instruments, the correct current is set by
navigating to the SRS field in the “Main” menu and using the “Select” keys to change the
setting. The readout is the approximate current level.
!
For optimal performance Dionex recommends the use of a Dionex RFC-10 or Dionex
RFC-30 power supply.
NOTE
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 79 of 84
Appendix B – Suppressor Controller
Appendix B – Suppressor Controller
B.1
Dionex ERS 500 and Dionex ERS 500e Control for the CD20/CD25/CD25A
and ED40/ED50/ED50A (Dionex DX-500 instruments)
Dionex ERS 500 and Dionex ERS 500e control for these instruments is accessible from the
detector front panel or Chromeleon software. For information on operation from Chromeleon,
please see the Help file in the Run or Method files.
!
If using the ED40, first select Conductivity Mode from the Main Screen.
NOTE
!
NOTE
There will not be an “ERS” setting on older instruments, use the SRS setting. The Dionex
ERS 500 and Dionex ERS 500e are fully backwards compatible with all SRS power
adapters and settings.
To operate the Dionex ERS 500 or Dionex ERS 500e from the front panel of these detectors:
A. Make sure the detector is in one of the main conductivity screens by choosing “Main
Screen” or “Detail Screen.” Press the “Menu” button until you see this as an option.
Press the appropriate number.
B. Make sure you are in the “Local” mode. If you are in the “Remote” mode use the arrow
keys to select “Local” and press “Enter.”
C. In the “Main” or “Detail” screens, navigate, using the arrow keys, to the field labeled
“SRS.” Set the current output level. Increase or decrease the levels by using the select
keys. These are labeled 50, 100, 300 and 500 mA. Remember to hit “Enter” after
choosing a setting.
!
CAUTION
Thermo Scientific
031956-11
1.
Dionex ERS 500 Control Connections for the CD20/ED40 (Dionex DX-500
instruments):
For these instruments, connect one end of the ERS control cable to slot 2, plug J3
behind the instrument front panel. Route the cable through the chase beneath the
electronics to the back. Route the female ERS plug end to the chromatography
module that you are using. Place the cable close to the ERS and attach.
2.
Dionex ERS 500 Line Voltage for the CD20/ED40 (DX-500 instruments):
These detectors have automatic switching power supplies to adjust to the line
voltage. No user settings are required.
3.
ERS Power Control for The CD20/ED40 (DX-500 instruments): The CD20 and
ED40 are controlled from the instrument front panel on DX-500 instruments.
Navigate to the SRS field and use the “Select” keys to scroll the current settings to
“Off.” Press “Enter.”
As a general operating precaution, never apply current to the suppressor without eluent or
water regenerant flowing through the suppressor at the same time. Always apply current
whenever eluent is running through the suppressor.
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 80 of 84
Appendix B – Suppressor Controller
B.1.1
Dionex ERS 500 Control for the Dionex DX-120
The Dionex ERS 500, Dionex ERS 500e and Dionex ERS 500 Carbonate are not compatible
with the Thermo Scientific Dionex DX-120 instrument. Suppressor control for this instrument is
only available via the use of a Dionex RFC-10 or Dionex RFC-30 suppressor controller.
!
Dionex recommends the use of a Dionex RFC-10 or Dionex RFC-30 power supply for the
Dionex DX-120 instrument.
NOTE
B.1.2
ERS Control for the CDM-3/PED-2 (Dionex DX-300 Instruments)
The Dionex ERS 500, Dionex ERS 500e and Dionex ERS 500 Carbonate are not compatible
with the Thermo Scientific Dionex DX-300 instrument. Suppressor control for this instrument is
only available via the use of a Dionex RFC-10 or Dionex RFC-30 suppressor controller.
!
Dionex recommends the use of a Dionex RFC-10 or Dionex RFC-30 power supply for the
Dionex DX-300 instrument.
NOTE
B.1.3
ERS Control for the Dionex DX-100 (Model DX 1-03)
The Dionex ERS 500, Dionex ERS 500e and Dionex ERS 500 Carbonate are not compatible
with the Thermo Scientific Dionex DX-100 instrument. Suppressor control for this instrument is
only available via the use of a Dionex RFC-10 or Dionex RFC-30 suppressor controller.
!
Dionex recommends the use of a Dionex RFC-10 or Dionex RFC-30 power supply for the
Dionex DX-100 instrument.
NOTE
B.1.4
SRC Controller
!
NOTE
Thermo Scientific Dionex recommends the Dionex RFC-10 (Item # 060335) for standalone
control of the Dionex ERS 500, Dionex ERS 500e or Dionex ERS 500 Carbonate in older
systems. The Dionex RFC-10 provides current at 1 mA intervals for exact current control.
This unit is intended to provide power and control to one Dionex ERS 500 or Dionex ERS 500e
when the associated detector does not have ERS control integrated into it.
The SRC-1 Power switch toggles On or Off AC line current to the SRC-1. Current is supplied to
the Dionex ERS 500 only as long as the SRC-1 is powered on.
!
The Dionex ERS 500 Carbonate is not compatible with the SRC Controller.
NOTE
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 81 of 84
Appendix B – Suppressor Controller
Figure B4
SRC-1 ERS Control
Power
1
0
Ready
Status
Alarm
Current
4
3
2
1
SRS CONTROLLER
!
NOTE
There is no “ERS” setting on the Dionex SRC-1, use the SRS setting. The Dionex ERS 500
and Dionex ERS 500e are fully backwards compatible with all SRS power adapters and
settings. For optimal performance a Dionex SCC-10 suppressor current controller should be
used in conjunction with the Dionex SRC-1 controller to provide a current output of twelve
settings.
The control and display panel of the Dionex SRC-1 is located on the front of that module’s
cabinet.
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 82 of 84
Appendix B – Suppressor Controller
A. SRC-1 Controller Connections
For the Dionex SRC-1 Controller, connect the Dionex SRC-1 six-foot output cable
directly to the Dionex ERS 500 current input connector.
B. SRC-1 Line Voltage
The 2-position rotary switch located on the back of the Dionex SRC-1 allows the
selection of either 110 V or 220 V input line voltage. It can be adjusted with a large
flat-bladed screwdriver or a small coin. The unit is not frequency sensitive and will
work equally well on 50 or 60 Hz without adjustment. Each position accepts a wide
range of input line voltages.
1.
Use the 110 V position for all input line voltages between 85 V ac and 135 V ac.
2.
Use the 220 V position for all input line voltages between 175 V ac and 265 V ac.
C. SRC-1 Power Control
The control and display panel on the Dionex SRC-1 is located on the front of that
module’s cabinet LED indicators inform the operator of the system’s operational
status.
The Dionex SRC-1 has its own AC Power On/Off switch to toggle the AC line current
to the Dionex SRC-1 on and off (see Figure B4, “SRC-1 ERS Control”). This is the
only way to turn the current output to the Dionex ERS 500 on and off on the Dionex
SRC-1.
Two line input fuses are located in the fuse drawer on the socket of the AC input
connector on the back panel of the Dionex SRC-1.
Figure B5
The SRC-1 Fuse Holder
Fuse
Drawer
Retaining
Clips
To replace fuses, first remove the AC line cord, and then remove the fuse drawer by squeezing
the retaining clips located at the right and left of the drawer. Pull the drawer outward to replace
both fuses. Spare fuses are found in the shipping kit. The fuses are 5 X 20 mm, 0.315A/250V,
low breaking capty FSF type (Item # 954747).
!
CAUTION
Thermo Scientific
031956-11
As a general operating precaution, never apply current to the suppressor without eluent or
water regenerant flowing through the suppressor at the same time. Always apply current
whenever eluent is running through the suppressor.
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 83 of 84
Appendix C – Alarm States For Older Instruments
Appendix C –
C.1
Alarm States for Older
Instruments
Alarm States for the CD20/ED40 (Dionex DX-500 Instruments)
When using these detectors, the power setting will be shown on the screen when the suppressor
is on. The suppressor cable must be connected to the controller and conductive liquid must be
flowing to prevent an alarm state.
When the ERS Control unit is in shutdown mode after detecting a malfunction in the system, an
alarm message “SRS Alarm” will flash on the screen. To determine the cause, press “Menu”
proceed to the “Diagnostic Menu” and press “Analog Status.” On the left side of the screen, two
fields give the ERS status as shown below:
SRS CONNECTED: Y (or N)
This indicates that the suppressor is connected. If it shows “N” connect the ERS cable.
SRS OVER VOLT: N (or Y)
This indicates whether the suppressor has a voltage higher than it can support (7.5 to 8 V). Try
lowering the power setting on the front panel. Also, see Section C.1.2, “The Alarm Red LED”
for other causes.
Thermo Scientific
031956-11
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
Page 84 of 84