Something missing What these rats don't have may be exactly what you want. ]'hey have no thyroid or pituitary glands, And thyroidectomies and hypophysectomies are only two of the six"endocrinectomies" we perform some 50,000 times a year on our COBS~ rats and mice and Lakeview hamsters. You can opt for one, some, or all of these surgical procedures. Even a neonatal thymectomy. The Charles River surgical team is made up entirely of graduate biologists. All experts in trauma-minimizing techniques and completeness of procedure. Which makes for optimum animal responsiveness. And to complement the surgery on our COBS animals, we're developing techniques to perform many procedures in germfree isolators, So when you need surgicalEy.altered animals, send us your specifications. Our take-out service is fast and reliable. ! Charles River BREEDtNG LABORATORIES, INC./ ~ WILMINGTON. M~Ct~U$1~-r$ 0|8~7 H~DOF~ " °t ~" "R' i A N V' ~ "~oRES~RCH THE JOURNAL OF IMMUNOLOGY BIOLOGY OF T H E IMMUNE RESPONSE Edited by Peter AbramoR, Marquette University and Mariano F. La Via, The Bowman Gray School of Medicine 1970, 492 pages, $12.95 Biology of the Immune Response fills the gap which exists in immunology today. While interest in immunology has grown at a rapid rate, until now there has been no book which presented factual information on immunological problems at a basic level and in a comprehensive manner. This book deals with the fundamental aspects of immunology at the cellular as well as the molecular level. It is biologically oriented. The up-to-date coverage of present literature and factual information makes this book a valuable asset to all those interested in immunology, physiology, pathology, genetics, and related fields. An outstanding feature of the text is its unified approach. In addition to the two principal authors, there are eleven contributing authors, each writing in his area of specialization. These authorities have worked in conjunction with Dr. Abramoff and Dr. La Via to provide an integrated, well-organized, and unified text. The unevenness of style and approach characteristic of many multi-authored works has been eliminated. McGraw-Hill Book Company 3 3 0 W. 4 2 n d St. New York, New York 1 0 0 3 6 This dry agarose film is the newest concept for immunodiffusion The SeaSep System: • A clear, rehydratable agarose film with pre-formed sample wells to save preparation time • Offers reproducibility • Is easily prepared by rehydration to a gel • Stable on shipping and storing • Comes in a plastic tray for ease of handling and storage • Prepared from standardized SeaKem Agarose T H E J O U R N A L OF I M M U N O L O G Y DEMONSTRABLY SUPERIOR ! AUTOMATIC 96-CHANNEL PIPETTER AUTOMATIC DILUTER ! O p e r a t i o n is p l u s / m i n u s 2 % a c c u r a t e f o r b o t h units. Each u n i t is p r o g r a m m a b l e . . , b o t h u n i t s a r e f u l l y i n t e g r a t e d w i t h t h e M i c r o t i t e r ~ s y s t e m . . , use e i t h e r p e r m a n e n t o r d i s p o s a b l e M i c r o t i t e r ~ plates. Complete descriptive brochure available. COOKE ENGINEERING 900 Slaters Lane • Alexandria, Va. 22314 • 703 / 548-3889 A DYNATECH COMPANY Our Trypsin i s a l l and stays a c t i v e Saves Mixing, Gray Process Trypsin gives all the cell stripping properties you're looking for, without the inconvenience of mixing, Saves Time and Effort: It's a sterilized and stabilized solution, that's ready when you are • . . without adding anything . . . the water's already there. No lab time lost while you prepare what you need. No time delay in thawing a frozen product. w e t stripping cells when for "Gray you're Process" ready to harvest, permits months! Saves Money; No waste due to inactivation of the trypsin ... it's stable for at least 90 days. The "Gray Process ''TM makes it all possible! "Gray Process" sterilizes and stabilizes T r y p s i n . . not altering its effectiveness in ~on,~,e,~ sterilization of sensirive protein solutions in the final container without destruction of the protein or alterations in product efficacy. In the case of Trypsin, "Gray Process" also stabilizes t h e solution without additives which may interfere with efficacy. Gray Process Trypsin Solution is available in 100 ml bottles, in 0.25% solution. Also available, Trypsin-EDTA Solution (0.25%-0.1%) in 100 rnl bottles. Samples are available for trial, so you can see ,"-"J r " r ~ " for yourself. Simpty fill out the coupon and ~ . . . , . ~ . _. we'll see you get a trial quant ty. See for yourself . . . . Our •= Trypsin is all wet! irypsln olution. the w e t w a s t e - s a v e r r I i ~ Gray industries, inc. ~ 2300 West Commercial E~lvd~, Ft. Lauderdale, Florid;~ 33309 ~ Gentlemen please send me: [ ] Samples of Trypsin Solution [ ] Samples of Trypsin-EDTA Solution [ ] Information on other animal sera Name: Title: Organization: Address: City & State: Zip: " :Jl.| I z!~ " I I I I I I I I I I I I I I I Hyland Immunonogy i~ii!~ii i~!!ii!i~!!~i!i!!ili!ii!i!~ Hyland announces the a v a i l a b i l i t y of t w o new a n t i h u m a n s e r u m s -IgD (6-chain specific) and IgE (~-chain s p e c i f i c ) - - b o t h p r o d u c e d in goat. Hyland also provides for t h e c u s t o m i m m u n i z a t i o n of goats or rabbits with investigator-supplied antigen. A l s o a v a i l a b l e is an e x t e n s i v e line of a n t i s e r u m s , f l u o r e s c e n t c o n j u g a t e d antiserums, IMMUNO-PLATE ® i m m u n o d i f f u s i o n plates and test kits for identification and q u a n t i t a t i o n of h u m a n serum proteins. Part of goat population at Hyland's 120-acre ranch at Murrieta, Calif. r- . . . . . ~ . . . . . . . . . . . . . iSend Today for complete product list HYLAND HYL/~ND DIVISION TRAVENOLLABORATORIES,INC. C o s t a Mesa, C a l i f o r n i a 9 2 6 2 6 3300 Hyland Ave., Costa Mesa, Calif. 92626 Please send me your IMMUNOLOGICAL REAGENTS Catalog JI Name Organization Street City. State Zip THE J O U R N A L OF IMMUNOLOGY NEWKohn electrophoresisunit has 1cm-21cmvariable bridge gap and economy . . . two words that best describe the unique new Shandon Electrophoresis Apparatus after Kohn. With a variable bridge gap from lcm to 21cm in lmm increments you get unrivaled economy with expensive media. You also can perform a wide range of techniques, including short distance HVE and countercurrent electrophoresis. Features include plugin polarity indicator and reversal switch, polarity indicator lights, full-length platinum electrodes, double-sloped transparent lid, and micro-switch safety attachment to lid for current cut-off. Special accessories are available for micro-immuno techniques. Size: 14" x 12" x 3½". In stock. Send for data sheet and complete catalog of TLC/TLE equipment to Shandon Scientific Company, Inc., 515 Broad Street, Sewickley, Pa. 15143 (Pittsburgh District). Versatility P I T T S B U R G H • Use this check list for other Shandon Catalogs and Monographs LONDON [] [] [] [] [] [] [] [] [] [] [] • FRANKFURT Paper Electrophoresis Acrylamide Gel Electrophoresis immuno Electrophoresis Thin Layer Electrophoresis High Voltage Electrophoresis Paper Chromatography Thin Layer Chromatography Preparative Layer Chromatography Automatic Slide Staining Machine Tissue Processing Apparatus Cyto-Centrifuge Please send your request for the above literature to Shandon Scientific Company, Inc., 515 Broad Street, Sewickley, Pa. 15143. THE JOURNAL OF IMMUNOLOGY CLINICAL A N D EXPERIMENTAL IMMUNOLOGY An Official Journal of THE B R I T I S H SOCIETY FOR I M M U N O L O G Y EDITOR: W. J. IRVINE Vol. 8, No. 2 F e b r u a r y 1971 CONTENTS P. RANL~tV and F. HARDT: In vitro evaluation of cell-mediated immunity in mice: experiments with soluble and cellular antigens in a spleen-thymus cell leucocyte migration test (LMT) A. DASGUPTA:Immune deviation: demonstration of split tolerance in vitro by inhibition of macrophage migration W. H. WOLBERG: Alternative pathways for I)NA-thymine synthesis in stimulated leucocytes P. TH. A. SCHELLEKENSand V. P. EIJSVOOGEL:Lymphocyte transformation in vitro. IV. Recruitment in antigen stimulated cultures E. SKAMENEand P. S. RUSSELL: A quantitative study of the binding of ALS to various cell types B. O. OSUNKOYAand A. L O. WILHAMS: Precipitation of serum proteins by phytohaemagglutinin J. C. DANIELS, H. SAKAL A. R. REMMEnS, JR., H. E. SARLES, J. C. FISH, E. K. COBB, W. C. LEVIN and S. E. RITZMaNN: In vitro reactivity of human lymphocytes in chronic uraemia: analysis and interpretation J. B. NATVIG,M. HARnOE, O. FAuS.~ and A. TWZlT: Family studies in individuals with absence of ~A-globulin M. T. CWYNAI~SKIand S. COHEN: Polyclonal immunogh)bulin deficiency in myelomatosis and macroglobulinaemia E. MUNT~E and J. B. NaTVIG: Characterization of IgG complexes in eluates from rheumatoid tissue B. R. ANDERSEN,J. IU McANINCH and R. PATTERSON: The incidence of precipitating activity in monoclonal proteins N. J. ZVAIFLERand MARIAM. MARTINEZ:Antinuclear factors and chronic articular inflammation H. BLOMGREN:Studies on the proliferation of thymus cells injected into syngeneic or allogeneic irradiated mice H. S. BONSTEIN and N. R. ROSE: Species-specific tissue antigens H. H. WORTIS: Immunological responses of 'nude' mice G. BIBERFELD:Antibodies to brain and other tissues in ca3es of Mycoplasma pneumoniae infection C. C. CURTAIN,B. L. CLARKand J. H. DUFTY: The origins of the immunoglobulins in the mucous secretions of cattle R. J. HUDSON,P. J. BANDYand W. 1). KITTS: [~ vitro detection of homocytotropic antibody in lungworm-infected rocky mountain bighorn sheep SHORT COMMUNICATION E. M. L. LEMMEL,E. R. HURD and M. ZIFF: Differential effects of 6-mercaptopurine and cyclophosphamide on autoimmune phenoma in NZB mice The subscription price in the United States and Canada is $54.00 for two annual volumes of six parts (single copies $5 each) B L A C K W E L L S C I E N T I F I C P U B L I C A T I O N S LTD OXFORD AND EDINBURGH Sharp Clear Accurate QUANTITATiON WITH cappel Ill... RADIAL IMMUNODIFFUSlON READER IMMUNODIFFUSION KITS .... 72 DETERMINATIONS OF . . . . . . . or or or or or or PROFILE KIT .... $ 5 5 . 0 0 each Human IgA HumanIgD HumanIgG HumanIgM HumanIgA (Pediatric) HumanIgG (Pediatric) HumanIgM (Pediatric) $55.00 18 DETERMINATIONS EACH OF: HumanIgA Human IgD Human tgG Human IgM S T A N D A R D C U R V E I N C L U D E D WITH EACH K I T IMMUNODIFFUSION PLATES AVAILABLE FOR OTHER PROTEINS AND OTHER SPECIES. Specialists in Fluorescein, Rhodamine, Ferritin and other chemical conjugations. cappel laboratories, OOWNINGTOWN, inc. PA. 19335, phone: (215) 269-0275 U.S.A. (215) 269-0500 THE JOURNAL OF IMMUNOLOGY Marcial-Rojas Here is the first reference compendium that covers all the maj~)r human protozoal and helminthic diseases with clinical correlates. Using 9 I0 magnificent photographs, the contributors give microscopic illustration of tissue response to the many diseases studied. All told, this book gives the pathologist a single source for study and diagnostic verification of these types of diseases, along with a good deal of added information about their carrying agents and treatment prognosis. One of the world's leading authorities on tropical diseases, Dr. Marcial-Rojas, prepared 10 chapters of the book. The remaining chapters were written by experts especially qualified to examine the pathology of particular disease strains. For the most part each author is writing about a disease he sees and treats on a daily basis. Pathology of Protozoal and Helminthic Diseases Raul A. Marcial-Rojas, M.D. D e p t . of P a t h o l o g y a n d Legal Medicine U n i v e r s i t y of P u e r t o R i c o School of M e d i c i n e 1 9 7 0 / a p p r o x . 890 p p . / 9 1 0 figs. $65.00 Williams & Wilkins 428 E. Preston St/Baltimore, Md. 21202 THE JOURNAL OF IMMUNOLOGY ~ Buffered Pancrestin Tablets ® A Convenient and Reliable Method for Sputum and Tissue Liquefaction. :~ Delicate bacteria such as the pneumococcus or H. influenza are not affected by treatment :~F Buffered pancreatin is non-cytolytic permitting pus cell counts of treated sputum :~¢ More rapid and profuse growth of M. TUBERCULOSIS MICROBIOLOGICAL REAGENTS IN TABLETS Fluid Thioglycollate Medium U.S.P. Uniformly Accurate Time and labor savings :~ ;": Preserves the cleanliness of your laboratory The most standardized and convenient method of preparing Fluid Thyoglycollate Medium for sterility testing according to U.S.P. Agar Agar -#3 Tablets It is no longer either economical or necessary to stock Bacteriological Culture Media in both broth and agar form. The new COLAB Price List is available upon request for prompt subscription attach label here service... Please use this coupon and attach a recent label in the space provided when writing to us about: • change of address • renewal • questions regarding your subscription Please use the space below to indicate your new address. Name Address City/State/Zip THE WILLIAMS & W I L K I N S C O M P A N Y 428 East Preston Street/Baltimore, Maryland 21202 THE J O U R N A L OF IMMUNOLOGY Let us solve your wait problem. T h e r e you are. S t a l l e d in the m i d s t of a vital research p r o j e c t because you've run out of essential biochemicals. So what h a p p e n s next? You could find yourself with a big, fat problem. Like a long, costly d e l a y a n d expensive down time while you sweat out the s n a i l ' s - p a c e delivery of the n e e d e d items. 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