Instruction Manual
Denville 300D
Microcentrifuge
Denville Scientific
PO Box 4588
Metuchen, NJ 08840
Phone: 908-757-7577
Fax: 908-757-7551
e-mail: [email protected]
Safety Precautions
NEVER use the centrifuge in any manner not specified in these instructions.
NEVER operate the centrifuge without a rotor properly attached to the shaft.
NEVER tighten rotor nut by hand only.
NEVER fill tubes while they are in the rotor. Liquid spillage may harm unit.
NEVER put hands in the rotor area unless the rotor is completely stopped.
NEVER move the centrifuge while the rotor is spinning.
NEVER use solvents or flammables near this or other electrical equipment.
NEVER centrifuge flammable, explosive or corrosive materials.
NEVER centrifuge hazardous materials outside of a hood or proper containment facility.
ALWAYS load the rotor symmetrically. Each tube should be counter balanced by another tube of the
same type and weight.
ALWAYS locate the centrifuge within easy access to an electrical outlet.
ALWAYS use only microcentrifuge tubes made from plastic and designed to withstand centrifugal
forces of at least 16,500 x g.
ALWAYS use a wrench to tighten the rotor nut.
Table of Contents
1. General Information
Description
Safety precautions
Technical data
Accessories supplied with unit
Warranty
1
2. Installation
Unpacking the centrifuge
Required space
Installing the centrifuge
2
3. Installing the Rotor
Rotors and accessories
Rotor maintenance
Removing and installing the angle rotor
Loading the rotor
Overloading rotors
3
4. Operation
6
Attaching rotor lid
Closing the lid
Lid release
Lid lock
Speed selection (RPM / g force)
Selection of operating time, momentary operation, Start/Stop
5. Service and Maintenance
Service
Cleaning
Disinfection
Replacing fuses
7
6. Troubleshooting
8
7. Where to Call
9
8. Determination of g-values
10
1. General Information
This manual provides important safety information for the laboratory microcentrifuge. It should
be kept near the centrifuge for quick and easy reference.
1.1 Description
The 300D is a small benchtop centrifuge designed for separation of various research
samples. The motor is brushless and requires no routine maintenance. The 300D is
supplied with a 24 x 1.5/2.0ml rotor for micro samples. Adapters are available for tubes
smaller than 1.5ml. The 300D reaches speeds of up to 13,300rpm/16,300 x g.
1.2 Safety precautions
Note: All users of the centrifuge must read the Safety Precautions section of this
manual before attempting to operate the unit!
If this equipment is used in a manner not specified by the manufacturer, the
protection provided by the equipment may be impaired.
Do not operate the centrifuge if any of the following conditions exist:
-The centrifuge has not been installed properly
-The centrifuge is partially dismantled
-Service has been attempted by unauthorized or unqualified personnel
-The rotor has not been installed securely on the motor shaft
-Rotors and accessories not belonging to the standard range are being used without
permission being obtained from the manufacturer to use such rotors and/or accessories in
the centrifuge
Exception: Microcentrifuge tubes made of plastic, normally available in the laboratory.
-The centrifuge is located in an explosive atmosphere
-Materials to be centrifuged are combustible and/or explosive
-Materials to be centrifuged are chemically reactive
-The rotor load is not properly balanced
- The rotor nut was not tightened with a wrench
1.3 Technical data
Dimensions
Width
Depth
Height
9.13 inches
12.25 inches
8.5 inches
Maximum speed
13,300rpm
Maximum RCF
16,300 x g
Maximum volume
24 x 1.5/2.0ml
Admissible sample density
1.2kg/dm3
Electrical/fuse rating
120V~, 50-60Hz, 1.9A, 2.5 AT
230V~, 50-60Hz, 1.1A, 1.25 AT
Operating temp./humidity
0ºC to 40ºC - 80%RH
1.4 Accessories supplied with centrifuge
Each unit is supplied with 1 instruction manual, 1 warranty card 1 power cord, a standard
rotor and a rotor removal tool.
1.5 Warranty
This centrifuge has been subjected to thorough testing and quality control. In the
unlikely event of a manufacturing fault, our one year warranty (from the date of delivery)
covers the centrifuge and the rotor. This warranty becomes invalid in the case of
incorrect operation, use of nonstandard spare parts or accessories and unauthorized
modification of the rotor or centrifuge.
Labnet reserves the right to make technical modifications. Please see the complete
limited warranty statement supplied separately.
2. Installation
2.1 Unpacking the centrifuge
Before unpacking the centrifuge, inspect the outside of the carton for any shipping
damage.
The centrifuge is delivered in a carton with protective foam cushioning. Remove the
centrifuge from the carton. Retain the carton and cushioning until it has been established
that the centrifuge is working properly. Inspect the centrifuge for any visible signs of
shipping damage. Shipping damage is the responsibility of the transportation carrier.
Any claims for damage must be filed within 48 hours with the carrier that delivered the
centrifuge. The accessories supplied with the centrifuge should be kept with the
instruction manual near the centrifuge’s place of installation.
2.2 Required space
The centrifuge should be installed on a rigid, even surface such as a stable laboratory
bench, countertop, etc. To guarantee sufficient ventilation, ensure that the centrifuge has
at least 15cm (6 inches) of free space on all sides, including the rear.
The centrifuge should not be located in areas subject to excessive heat such as in direct
sunlight or near radiators or the exhaust of a compressor, as a buildup of heat may occur
within the chamber.
2.3 Installation
Before operating the centrifuge, check that the power source (electrical outlet on the wall)
corresponds to that on the manufacturer’s rating label, then connect the power cord to the
centrifuge and the power source.
3. Installation of rotors and rotor maintenance
3.1 Rotors and accessories
The following accessories are included or available for the 300D:
Angle rotor for 24 x 1.5ml tubes (Included)
Tube measurement
1.5ml / 2.0ml (10 x 40mm)
Max. speed
13,300rpm
Centrifuging radius
8.23cm
Max. RCF (g-value)
16,276 x g
Rotor Wrench
Order no.
(Included)
C2400-RT
Rotor Lid
Order no.
(Included)
C2400-RC
Rotor Securing Screw
(Included)
Order no.
C2400-RSS
Strip Spin Adapter
Order no.
C2400-SS
Tubes accepted
0.2ml tubes or 8 x 0.2ml strips
Max Speed
13,300 rpm
Range Centrifuge radius
4.32cm to 5.33cm
Range RCF (g value)
8,549 x g to 10,539 x g
Adapter for 0.5ml tubes (pk./6)
Order no.
C1205
Tube measurement
8 x 30mm
Max. speed
13,300rpm
Centrifuging radius
7.53cm
RCF (g-value)
14,892 x g
Adapter for 0.4ml tubes (pk./6)
Order no.
C1206
Tube measurement
Max. speed
Centrifuging radius
RCF (g-value)
6 x 47mm
13,300rpm
8.23cm
16,276 x g
Adapter for 0.2ml tubes (pk./6)
Order no.
C1222
Tube measurement
6 x 21mm
Max. speed
13,300rpm
Centrifuging radius
7.03cm
RCF (g-value)
13,903 x g
3.2 Rotor maintenance
The rotor should be cleaned thoroughly after each use. Thorough cleaning must be
performed when spinning samples containing phenol or phenol chloroform.
Periodically inspect the rotor for dents, dings, scratches, discoloration and cracks. If any
damage to the rotor is found, discontinue use of the rotor immediately and replace.
3.3 Removing and installing the angle rotor
The 300D comes complete with a standard 24 place rotor installed. To remove the rotor
for cleaning, remove the rotor securing screw from the motor shaft by turning the screw
counterclockwise, using the rotor wrench. Lift the rotor directly upward in a straight
vertical motion. To replace rotor, first make sure the motor shaft and rotor mounting hole
are clean. Place the rotor on the motor shaft. Reinstall the rotor securing screw on the
motor shaft by turning it clockwise. Hold the rotor with one hand and tighten the rotor
securing screw, using the rotor wrench.
Figure 1. Loading the rotor to achieve balance
3.4 Loading the rotor
Tubes to be loaded should be filled equally by eye. The difference in the weight between
the tubes should not exceed 0.1 gram. Tubes should always be loaded so that there is
equal spacing between all tubes. One or two additional loaded tubes may need to be
added to achieve this. Refer to Figure 1. to see one typical balancing scheme.
3.5 Overloading rotors
The maximum load of the rotor and the maximum speed has been established by the
manufacturer. Do not attempt to exceed these values. The maximum speed of the rotor
has been established for liquids having a homogeneous density of 1.2g/ml or less. In
order to centrifuge liquids with a higher density it is necessary to reduce the speed.
Failure to reduce the speed may result in damage to the rotor and centrifuge. The
revised maximum speed can be calculated with the following formula:
Reduced speed (nred) =
x max speed (nmax)
Example:
Where the density of the liquid is 1.7, the new maximum speed would be calculated as
follows:
nred =
x 13,300 = 11,174 rpm
If in doubt concerning maximum speeds, please contact the manufacturer for assistance.
4. Operation
ATTENTION: Never attempt to operate the centrifuge with rotors or adapters that
show signs of corrosion or mechanical damage. Never centrifuge strongly corrosive
materials that may damage the rotors, accessories, or bowl of the unit.
4.1 Attaching rotor lid
After the rotor has been properly secured and loaded, attach the rotor lid to the rotor.
Always use the rotor lid for safety and to allow the rotor to reach proper speed. Make
sure that the rotor lid snaps securely into place, by pressing down on center catch.
4.2 Closing the lid
Close the centrifuge lid. The 300D has a lid lock that activates only when a run is started.
4.3 Lid release
The lid will remain locked during a centrifuge run. Once the run has been completed and
the rotor has come to a stop, a beep will indicate the end of a run, and the lid will unlock
automatically.
WARNING: Do not attempt to open the lid of any centrifuge until the rotor has
come to a complete stop.
In the event of a power failure or malfunction, it may be necessary to open the lid
manually.
1. Disconnect the power cord from the wall socket.
2. Remove the plastic plug, located on the left side of the unit.
3. Pull the wire (attached to the plug) to open the lid lock manually.
4.4 Lid lock
The centrifuge can be started only with the lid securely closed. When a run is started, the
lid lock automatically activates. Do not attempt to open the lid during a centrifuge run.
At the end of the run, the lid will automatically unlock.
Never attempt to override the lid lock mechanism. Doing so is dangerous and could
damage the centrifuge.
4.5 Speed selection (see Figure 2.)
The speed (rpm or g-force) can be selected from 500 to 13,300 rpm in 100 rpm
increments or from 100 to 16,300 x g with the knob on the right. “g-force” or “rpm”
display is set by pressing the knob. Turn the knob to increase or decrease the value.
4.6 Selection of operating time, momentary operation,
Start/Stop
Operating time can be selected from 0.5 min to 30 min by turning the timer knob on the
left. The time can be set in 0.5 minute increments from 0 to 10 minutes and in 1 minute
increments from 10 to 30 minutes. After 30 minutes, the display shows “--” which
indicates continuous run. In this mode, the centrifuge will run until manually stopped.
To start a run, press the timer knob.
When the pre-selected time expires, the centrifuge will stop automatically. To stop the
centrifuge prior to the expiration of set time, press the timer knob.
The centrifuge may be operated for a short run by pressing and holding the timer knob.
The centrifuge will continue to run as long as the timer knob is depressed and the time, in
seconds will count up on the time display.
Figure 2. 300D control panel layout
5. Service and Maintenance
5.1 Centrifuge service
The brushless motor in the 300D requires no routine maintenance. Any required service
should be performed by authorized, qualified personnel only. Repairs performed by
unauthorized personnel may void the warranty.
5.2 Cleaning the centrifuge
Always keep the centrifuge housing, rotor chamber, rotor and rotor accessories clean. All
parts should be wiped down periodically with a soft cloth. For more thorough cleaning,
use a neutral cleaning agent (pH between 6 and 8) applied with a soft cloth. Excessive
amounts of liquid should be avoided. Liquid should not come into contact with the
motor. After cleaning, ensure that all parts are dried thoroughly by hand or in a warm air
cabinet (maximum temperature 50ºC)
5.3 Cleaning the rotor
The rotor should be cleaned after each use. When spinning samples containing phenol or
phenol chloroform, the rotor should be cleaned immediately after use.
5.4 Disinfection
Should a spill of infectious materials occur within the rotor or chamber, the unit should
be disinfected. This should be performed by qualified personnel with proper protective
equipment.
5.5 Replacing fuses
Check the fuse when it is recommended in the Troubleshooting Guide located in this
manual. The fuse holder is located in the power inlet on the rear of the unit. Disconnect
the power cord from the power inlet. Open the fuse holder drawer by inserting a small
screwdriver under the tab and prying it open. Remove the innermost (operative) fuse
from its retaining tabs and replace the fuse if necessary. A spare fuse is located in the
outermost chamber of the fuse drawer. Replace only with a fuse of exactly the same
value as the original. (Fuse type may be found in the Technical data section of this
manual.)
6. Troubleshooting Guide
Please refer to this guide before calling for service.
Centrifuge will not start
Possible reason:
Solution:
No power supply
Check that power is being supplied to
the outlet
Check that the power cord is plugged
into both the wall outlet and the back of
the centrifuge
Check that power cord is not damaged
Possible reason:
Solution:
Blown fuse
Check fuse and replace if necessary
Lid lock will not release
Possible reason:
Solution:
Defective lid lock
Open manually and have unit serviced
Possible reason:
Solution:
No power from PC board
Call for service
Possible reason:
Solution:
Lid lock is jammed
Call for service
Possible reason:
Solution:
Centrifuge is not receiving power
See “Centrifuge will not start”
Centrifuge cannot be started, although power is on
Possible reason:
Solution
Lid not closed correctly
Close lid correctly
Possible reason:
Solution:
No speed or time has been selected
Set speed and/or time
bAL: Error Message
Possible reason:
Solution:
Possible reason:
Indicates imbalance
Tubes not inserted symmetrically in rotor
holes
Load tubes symmetrically
(see Section 3.4 on Loading the rotor)
Sample liquid in tubes not balanced
Solution:
Make sure the same volume of liquid is
in each tube
Possible reason:
Defective or improperly adjusted
balance sensor
Call service
Solution:
Lid: (Error message)
Lid not closed
Possible reason:
Solution:
Lid not closed completely
Close lid
Possible reason:
Solution:
Lid lock or sensor defective
Call service
Other Error Messages
Solution:
Er on Display
Press time or speed knob to clear error
Call service
7. Where to call
Should you have any questions about the 300D or its accessories, please call Labnet’s
Customer Service Department at 732 417-0700. Customer Service is staffed from 8:30am to
5:30pm, EST, Monday through Friday. Our 24 hour fax number is 732 417-1750. Inquiries
may also be sent via our electronic mailbox at [email protected].
Should your 300D require service, please call Labnet’s Technical Services Department at 732
417-0700. Our Service Department is staffed from 8:30am to 5:00pm, EST, Monday through
Friday. Our 24 hour fax number is 732 417-1750. Electronic mail may be sent to
[email protected].
Please have the unit’s serial number (located on the back panel of the instrument)
available when calling. Should an item require return to Labnet for service, a repair
authorization (RA) number must first be received from Labnet and a decontamination
form completed by the user. Items sent without an RA number or decontamination form
will not be accepted.
8. Determination of g-values
The centrifuging radius of the 1.5/2.0ml rotor is 8.23cm. The 300D has an automatic g-force
conversion program, so g- values are automatically calculated and can be displayed on the
control panel, for this centrifuging radius. If adapters or smaller tubes are used, the
centrifuging radius changes as does the g force. The chart on the next page can be used to
manually determine g-values for any centrifuging radius.