How to get more information?
SPE Application Guide
Chromatography Catalogue
Contact your Local Distributor:
e-mail: Germany
and international:
Switzerland:
France:
USA:
SPE Catalogue
[email protected]
[email protected]
[email protected]
[email protected]
SPE06 en1/8/0/2.2006 PD
Printed in Germany
For a Clear Solution . . .
Online Application Database
MACHEREY-NAGEL
MACHEREY-NAGEL GmbH & Co. KG · Neumann-Neander-Str. 6-8 · D-52355 Düren · Tel. +49 (0) 24 21 969-0 · Fax +49 (0) 24 21 969 199
Switzerland: MACHEREY-NAGEL AG · Postfach 214 · CH-4702 Oensingen · Tel. +41 (0) 62 388 55 00 · Fax +41 (0) 62 388 55 05
France:
MACHEREY-NAGEL EURL · 1, rue Gutenberg · B.P. 135 · F-67722 Hoerdt · Tel. +33 (0) 3 88 68 22 68 · Fax +33 (0) 3 88 51 76 88
USA:
MACHEREY-NAGEL Inc. · 6 South Third St., Suite 402 · Easton, PA 18042 · Tel. +1 610 559 9848 · Fax +1 610 559 9878
www.mn-net.com
MN
Ready-to-use Cartridges for Modern Sample Preparation
MACHEREY-NAGEL
www.mn-net.com
MN
SPE_S.2-3.pdf
22.03.2006
12:00:43 Uhr
SPE_S.2-3.pdf
22.03.2006
10:52:35 Uhr
Fo o
Ur
in
e
d
Drinking water
T
B ablet
W
s
as loo
d
te
oi
l
What is SPE?
il
So
Ve
ge
ta
b
le
Fruit
Solid Phase Extraction (SPE) is an excellent method for sample preparation in analytical
chemistry.
CHROMABOND® and CHROMAFIX® SPE products from MN are particularly designed to
➢ prepare samples for analysis with HPLC, GC, TLC, UV and IR spectroscopy
➢ concentrate the substance to be analysed
➢ remove interfering substances
➢ changing the matrix of the analyte as needed for the subsequent analysis
➢ replace classical liquid-liquid extractions
Which SPE products does MACHEREY-NAGEL offer?
CHROMAFIX®
cartridges
for small sample volumes
with luer fittings
(0.4 – 1.8 ml)
How does SPE work?
Retention of the analyte
Retention of interfering components
CHROMABOND®
polypropylene columns
C
established material and design
acknowledged for all applications
(1 – 150 ml; 30 mg – 50 g)
M
Pesticides
PCB
PAH
Drugs
Dyes
Vitamins
Y
CM
MY
CY
CMY
K
conditioning
sample application
analyte
washing
elution
interfering component
– sample is pressed or drawn through the solid phase
– analyte molecules are enriched on the adsorbent
– interfering components and solvent molecules
(matrix) are not retained
– remaining interfering components are washed from
the adsorbent
– the analyte is removed from the adsorbent by
elution with a suitable solvent
conditioning
sample application
analyte
for ultrapure SPE
resistant to all solvents
(1, 3, 6 ml; 30 – 2000 mg)
CHROMABOND® LV
(large volume)
design for large volume samples
established for clinical samples
and Zymate® robots
(15 ml; 100 – 500 mg)
elution
interfering component
– sample is pressed or drawn through the solid phase
– analyte molecules show no interaction with the
adsorbent
– interfering components and solvent molecules
(matrix) are retained
– analyte molecules are „washed“ from the adsorbent
– the solid phase is simply used to „filter“ the sample
Who can use SPE?
CHROMABOND® Multi 96
for simultaneous and automated
preparation of 96 samples
(25 – 100 mg)
➢ any analytical laboratory can employ SPE and its benefits
➢ environmental agencies
➢ clinical laboratories
➢ food control laboratories and food producers
MN
CHROMABOND®
glass columns
CHROMABOND®
manifolds and accessories
for simultaneous preparation of 12, 16 or 24 samples
www.mn-net.com
www.mn-net.com
CHROMABOND®
tailor made solutions
aside standard dimensions and fillings, different
column sizes and adsorbent weights can be made
available in accordance to customers wishes
MN
SPE_S.2-3.pdf
22.03.2006
10:52:35 Uhr
d
Foo
Ur
in
e
Drinking water
T
B ablet
W
s
as loo
d
te
oi
l
What is SPE?
il
So
Ve
ge
ta
b
le
Fruit
Solid Phase Extraction (SPE) is an excellent method for sample preparation in analytical
chemistry.
CHROMABOND® and CHROMAFIX® SPE products from MN are particularly designed to
➢ prepare samples for analysis with HPLC, GC, TLC, UV and IR spectroscopy
➢ concentrate the substance to be analysed
➢ remove interfering substances
➢ changing the matrix of the analyte as needed for the subsequent analysis
➢ replace classical liquid-liquid extractions
Which SPE products does MACHEREY-NAGEL offer?
CHROMAFIX®
cartridges
for small sample volumes
with luer fittings
(0.4 – 1.8 ml)
How does SPE work?
Retention of the analyte
Retention of interfering components
CHROMABOND®
polypropylene columns
C
established material and design
acknowledged for all applications
(1 – 150 ml; 30 mg – 50 g)
M
Pesticides
PAH
PCB
Drugs
Dyes
Vitamins
for ultrapure SPE
resistant to all solvents
(1, 3, 6 ml; 30 – 2000 mg)
Y
CHROMABOND® LV
(large volume)
design for large volume samples
established for clinical samples
and Zymate® robots
(15 ml; 100 – 500 mg)
CM
conditioning
sample application
analyte
washing
interfering component
elution
MY
conditioning
sample application
analyte
elution
interfering component
CY
– sample is pressed or drawn through the solid phase
CMY
– analyte molecules are enriched on the adsorbent
K
– interfering components and solvent molecules
(matrix) are not retained
– remaining interfering components are washed from
the adsorbent
– the analyte is removed from the adsorbent by
elution with a suitable solvent
– sample is pressed or drawn through the solid phase
– analyte molecules show no interaction with the
adsorbent
– interfering components and solvent molecules
(matrix) are retained
– analyte molecules are „washed“ from the adsorbent
– the solid phase is simply used to „filter“ the sample
Who can use SPE?
CHROMABOND® Multi 96
for simultaneous and automated
preparation of 96 samples
(25 – 100 mg)
➢ any analytical laboratory can employ SPE and its benefits
➢ environmental agencies
➢ clinical laboratories
➢ food control laboratories and food producers
MN
CHROMABOND®
glass columns
CHROMABOND®
manifolds and accessories
for simultaneous preparation of 12, 16 or 24 samples
www.mn-net.com
www.mn-net.com
CHROMABOND®
tailor made solutions
aside standard dimensions and fillings, different
column sizes and adsorbent weights can be made
available in accordance to customers wishes
MN
11:56:12 Uhr
What are the benefits of
CHROMABOND® products?
➢ high recovery rates
➢ excellent reproducibility of
results
➢ improved selectivity of the
analysis
➢ reducing consumption of
organic solvents
➢ speeding up analysis time
➢ specific selectivities for
particular applications
➢ over 40 different phases
C
22.03.2006
11:26:42 Uhr
Selection of CHROMABOND® products
CHROMABOND® polypropylene columns
polar modified polymer resin (PS/DVB)
Cat. No.
column size
1 ml
3 ml
6 ml
adsorbent
30 mg 100 mg 60 mg 200 mg 500 mg
pack of
30
30
30
EASY
730751
modified silica
column size
1 ml
adsorbent
100 mg
pack of
100
730752
730753
3 ml
200 mg 500 mg
50
730754
730756
Cat. No.
6 ml
500 mg 1000 mg
30
C18 ec
730011
730012
730013
730014
730015
C18 Hydra
730294
730296
730297
730299
730300
M
Y
NH2
730031
730413
730033
730180
730626
SiOH
730071
730214
730073
730070
730075
CM
MY
CY
CMY
SA (SCX)
730076
730275
730077
730425
730212
SB (SAX)
730078
730322
730079
730426
730323
Florisil®
730089
730457
730081
730238
730082
Drug
730681
730168
730684
730682
Drug II
730685
730680
730686
730683
K
To maintain product quality and to
realise long shelf-lifes, all SPE
products are sealed in laminated
aluminium bags or blistered, which
protect the sorbents from contact with
environment and thus prevent
contamination.
MN
Vacuum manifold
complete (12 positions)
730150
www.mn-net.com
How to find the right CHROMABOND® phase!
Selection guide for SPE phases and solvents
Our Product range:
Standard phases
polymer resins
(PS/DVB)
EASY
HR-P
PS-RP
modified silica
C18 ec / ec f
C18 / f
C18 Hydra
C8
C4
C2
C6H11 ec
C6H5
NO2
NH2
DMA
CN
OH
PA
SiOH
Alox A, N, B
Florisil®
non polar
SPE_S.4-5_NEW.pdf
middle polar
22.03.2006
Special phases
Drug
Drug II
ABC18
Diamino
Tetracycline
Crosslinks
AOX
CN/SiOH
C18 PAH
NH2 / C18
Na2SO4 / Florisil®
SiOH-H+/SA
SA/SiOH
NAN
DNPH
Dry
XTR
Sample
solubility
Solvent
not
ionic
aqueous
soluble in
water
Sample
polarity
Phases recommended
for adsorption
nonpolar
Easy, HR-P
C18 ec, C18, C18 Hydra
C8, C4, C2, C6H5, CN
alcohols
acetonitrile
hexane
CH2Cl2
moderately
polar
Easy, HR-P
C18 ec, C18, C18 Hydra
C8, C4, C2, C6H5, CN
alcohols
ethyl acetate
CHCl3, CH2Cl2
CN, OH
PA
DMA
NH2
alcohols
ethyl acetate
CHCl3, CH2Cl2
polar
ionic
ion exchanger
SA (SCX)
SB (SAX)
PCA (WCX)
PSA
PS-OH–
PS-H+
PS-Ag+
PS-Ba2+
www.mn-net.com
soluble in
organic
solvents
Solvents recommended for elution (selection)
cationic
PCA (WCX)
SA (SCX)
PSA
PS-H+
acids
salt solutions
buffers
anionic
SB (SAX)
NH2
DMA
PS-OH–
acids
salt solutions
buffers
Easy, HR-P, PS-RP
C18 ec, C18, C18 Hydra
C8, C4, C2, C6H5, CN
alcohols
acetonitrile
hexane
CH2Cl2
SiOH
NH2
alcohols
ethyl acetate
CHCl3, CH2Cl2
CN, OH
PA
DMA
NH2
alcohols
ethyl acetate
CHCl3, CH2Cl2
aqueous
polar
SPE_S.4-5_NEW.pdf
aqueous
nonpolar
organic
moderately
polar
organic
polar
MN
C
Selection of CHROMABOND® products
CHROMABOND® polypropylene columns
polar modified polymer resin (PS/DVB)
Cat. No.
column size
1 ml
3 ml
6 ml
adsorbent
30 mg 100 mg 60 mg 200 mg 500 mg
pack of
30
30
30
EASY
730751
730752
730753
modified silica
column size
adsorbent
pack of
1 ml
100 mg
100
3 ml
200 mg 500 mg
50
730754
730756
Cat. No.
6 ml
500 mg 1000 mg
30
C18 ec
730011
730012
730013
730014
730015
C18 Hydra
730294
730296
730297
730299
730300
M
Y
NH2
730031
730413
730033
730180
730626
SiOH
730071
730214
730073
730070
730075
CM
MY
CY
CMY
SA (SCX)
730076
730275
730077
730425
730212
SB (SAX)
730078
730322
730079
730426
730323
Florisil®
730089
730457
730081
730238
730082
Drug
730681
730168
730684
730682
Drug II
730685
730680
730686
730683
K
To maintain product quality and to
realise long shelf-lifes, all SPE
products are sealed in laminated
aluminium bags or blistered, which
protect the sorbents from contact with
environment and thus prevent
contamination.
MN
Vacuum manifold
complete (12 positions)
730150
www.mn-net.com
How to find the right CHROMABOND® phase!
Selection guide for SPE phases and solvents
Our Product range:
Standard phases
polymer resins
(PS/DVB)
EASY
HR-P
PS-RP
modified silica
C18 ec / ec f
C18 / f
C18 Hydra
C8
C4
C2
C6H11 ec
C6H5
NO2
NH2
DMA
CN
OH
PA
SiOH
Alox A, N, B
Florisil®
non polar
➢ high recovery rates
➢ excellent reproducibility of
results
➢ improved selectivity of the
analysis
➢ reducing consumption of
organic solvents
➢ speeding up analysis time
➢ specific selectivities for
particular applications
➢ over 40 different phases
11:26:42 Uhr
middle polar
What are the benefits of
CHROMABOND® products?
22.03.2006
Special phases
Drug
Drug II
ABC18
Diamino
Tetracycline
Crosslinks
AOX
CN/SiOH
C18 PAH
NH2 / C18
Na2SO4 / Florisil®
SiOH-H+/SA
SA/SiOH
NAN
DNPH
Dry
XTR
Sample
solubility
Solvent
not
ionic
aqueous
soluble in
water
Sample
polarity
Phases recommended
for adsorption
nonpolar
Easy, HR-P
C18 ec, C18, C18 Hydra
C8, C4, C2, C6H5, CN
alcohols
acetonitrile
hexane
CH2Cl2
moderately
polar
Easy, HR-P
C18 ec, C18, C18 Hydra
C8, C4, C2, C6H5, CN
alcohols
ethyl acetate
CHCl3, CH2Cl2
CN, OH
PA
DMA
NH2
alcohols
ethyl acetate
CHCl3, CH2Cl2
polar
ionic
ion exchanger
SA (SCX)
SB (SAX)
PCA (WCX)
PSA
PS-OH–
PS-H+
PS-Ag+
PS-Ba2+
www.mn-net.com
soluble in
organic
solvents
Solvents recommended for elution (selection)
cationic
PCA (WCX)
SA (SCX)
PSA
PS-H+
acids
salt solutions
buffers
anionic
SB (SAX)
NH2
DMA
PS-OH–
acids
salt solutions
buffers
Easy, HR-P, PS-RP
C18 ec, C18, C18 Hydra
C8, C4, C2, C6H5, CN
alcohols
acetonitrile
hexane
CH2Cl2
SiOH
NH2
alcohols
ethyl acetate
CHCl3, CH2Cl2
CN, OH
PA
DMA
NH2
alcohols
ethyl acetate
CHCl3, CH2Cl2
aqueous
polar
SPE_S.4-5_NEW.pdf
aqueous
nonpolar
organic
moderately
polar
organic
polar
MN
SPE_S.6-7_END.pdf
22.03.2006
12:01:36 Uhr
Biological samples and
natural compounds
Environmental samples
and pollutants
Application
Phase
Flavonoids from plants
Prostaglandins from urine
Nicotine from urine
Fatty acids from serum
etc.
PA
C18ec/SiOH
C18ec
C18ec
Appl. No.
300150
300510
300070
300270
Application
Phase
PCB from waste oil
Pesticides from water
16 PAH from soil (EPA)
PAH from drinking water (EPA 550)
etc.
SA-SiOH
HR-P
Easy
C18ec
Appl. No.
301390
301550
302820
302170
Food and beverages
Pharmaceuticals and drugs
Application
Phase
Appl. No.
Drugs of abuse from serum
Antbiotics
Anesthetics from serum
Antidepressants from urine
etc.
Drug
Easy
C18
C18ec
302240
302770
300710
300850
SPE_S.6-7_END.pdf
22.03.2006
Application
Phase
Appl. No.
Vitamins from food
Pesticides from citrus fruits
Mycotoxins from cereals
Antibiotics in milk
etc.
XTR
OH
SiOH
C18ec
303720
301500
300410
303730
11:37:38 Uhr
Some highlights of a wide range of applications:
Phase selection by Manufacturer
C
M
MACHEREY-NAGEL* Phenomenex
CHROMABOND®
STRATA
Y
CM
Waters
Sep-Pak
Varian
Supelco
Bond Elut Discovery
Supelclean
UCT
C18-E
C18-U
tC-18
C18
C18
C8
Phenyl (PH)
SDB-L
C8
strata-X
Oasis HLB
Porapak RDX
MY
Agilent
Accubond
JT BAKER
Bakerbond
IST
Isolute
Octadecyl
PolarPlus
Octadecyl Lightload
Octyl
Phenyl
H2O-phobic DVB
C18(EC)
C18
C8(EC)
PH(EC)
101
H2O-philic DVB
ENV+
Silica-gel
Florisil®
Amino
Cyano
Silica
FL
NH2
CN
Reversed Phase
CY
CMY
C18ec
C18
C18 PAH
C8
C6H5
HR-P
PS-RP
Easy
K
MN
18
C18
C8
PH
ENV
LMS
NEXUS
PPL
Focus
8
PH
PS/DVB
C8
Phenyl
C8
Phenyl
Si-1 (SiIica)
FL-PR (Florisil®) Florisil®
NH2
NH2
CN
CN
Silica
Florisil®
NH2
CN-U
Silica
SAX
SCX
strata-X-C
SAX
SCX
SAX
SCX
Quarternary Amine
SAX
Benzene Sulfonic Acid SCX
StyreScreen® DBX
Ouarternary Amine
Aromatic Sulfonic Acid
SAX
SCX
CBA
Certify I
Certify II
WCX
Carboxylic Acid
Clean Screen DAU
Clean Screen THC
Carboxylic Acid
Narc-2
Narc-1
CBA
HCX
HAX
StyreScreen® DVB
Normal Phase
SiOH
Florisil®
NH2
CN
NH2
CN
Silica
Florisil®
Amino propyl
CN
Silica
Envi-Florisil®
Amino
Cyano
Ion Exchanger
SB
SA
PS-H+
PS-OH–
PCA
Drug
Drug II
Complete details for these and over 300
other applications can be found in our:
SPE Application Guide
and in our on-line application data base:
Evidex
*Recommended Alternative - This category indicates a phase which may yield somewhat different selectivity but may also lead to improved resolution.
www.mn-net.com
MN
WCX
Screen-C
Screen-A
Oasis MCX
Oasis MAX
www.mn-net.com
www.mn-net.com
MN
Biological samples and
natural compounds
Environmental samples
and pollutants
Application
Phase
Flavonoids from plants
Prostaglandins from urine
Nicotine from urine
Fatty acids from serum
etc.
PA
C18ec/SiOH
C18ec
C18ec
Appl. No.
300150
300510
300070
300270
Application
Phase
PCB from waste oil
Pesticides from water
16 PAH from soil (EPA)
PAH from drinking water (EPA 550)
etc.
SA-SiOH
HR-P
Easy
C18ec
Appl. No.
301390
301550
302820
302170
Food and beverages
Pharmaceuticals and drugs
Application
Phase
Appl. No.
Drugs of abuse from serum
Antbiotics
Anesthetics from serum
Antidepressants from urine
etc.
Drug
Easy
C18
C18ec
302240
302770
300710
300850
SPE_S.6-7_END.pdf
22.03.2006
Application
Phase
Appl. No.
Vitamins from food
Pesticides from citrus fruits
Mycotoxins from cereals
Antibiotics in milk
etc.
XTR
OH
SiOH
C18ec
303720
301500
300410
303730
11:37:38 Uhr
Some highlights of a wide range of applications:
Phase selection by Manufacturer
MACHEREY-NAGEL* Phenomenex
STRATA
CHROMABOND®
Waters
Sep-Pak
Varian
Supelco
Bond Elut Discovery
Supelclean
UCT
C18-E
C18-U
tC-18
C18
C18
C8
Phenyl (PH)
SDB-L
C8
strata-X
Oasis HLB
Porapak RDX
Agilent
Accubond
JT BAKER
Bakerbond
IST
Isolute
Octadecyl
PolarPlus
Octadecyl Lightload
Octyl
Phenyl
H2O-phobic DVB
C18(EC)
C18
C8(EC)
PH(EC)
101
H2O-philic DVB
ENV+
Silica-gel
Florisil®
Amino
Cyano
Silica
FL
NH2
CN
Reversed Phase
C18ec
C18
C18 PAH
C8
C6H5
HR-P
PS-RP
Easy
C
MN
18
C18
C8
PH
ENV
LMS
NEXUS
PPL
Focus
8
PH
PS/DVB
Si-1 (SiIica)
FL-PR (Florisil®) Florisil®
NH2
NH2
CN
CN
Silica
Florisil®
NH2
CN-U
Silica
SAX
SCX
strata-X-C
SAX
SCX
SAX
SCX
Quarternary Amine
SAX
Benzene Sulfonic Acid SCX
StyreScreen® DBX
Ouarternary Amine
Aromatic Sulfonic Acid
SAX
SCX
CBA
Certify I
Certify II
WCX
Carboxylic Acid
Clean Screen DAU
Clean Screen THC
Carboxylic Acid
Narc-2
Narc-1
CBA
HCX
HAX
M
Y
C8
Phenyl
C8
Phenyl
StyreScreen® DVB
Normal Phase
CM
MY
SiOH
Florisil®
NH2
CN
CY
CMY
K
NH2
CN
Silica
Florisil®
Amino propyl
CN
Silica
Envi-Florisil®
Amino
Cyano
Ion Exchanger
SB
SA
PS-H+
PS-OH–
PCA
Drug
Drug II
Complete details for these and over 300
other applications can be found in our:
SPE Application Guide
and in our on-line application data base:
Evidex
*Recommended Alternative - This category indicates a phase which may yield somewhat different selectivity but may also lead to improved resolution.
www.mn-net.com
MN
WCX
Screen-C
Screen-A
Oasis MCX
Oasis MAX
www.mn-net.com
www.mn-net.com
MN
How to get more information?
SPE Application Guide
Chromatography Catalogue
Contact your Local Distributor:
e-mail: Germany
and international:
Switzerland:
France:
USA:
SPE Catalogue
[email protected]
[email protected]
[email protected]
[email protected]
SPE06 en1/8/0/2.2006 PD
Printed in Germany
For a Clear Solution . . .
Online Application Database
MACHEREY-NAGEL
MACHEREY-NAGEL GmbH & Co. KG · Neumann-Neander-Str. 6-8 · D-52355 Düren · Tel. +49 (0) 24 21 969-0 · Fax +49 (0) 24 21 969 199
Switzerland: MACHEREY-NAGEL AG · Postfach 214 · CH-4702 Oensingen · Tel. +41 (0) 62 388 55 00 · Fax +41 (0) 62 388 55 05
France:
MACHEREY-NAGEL EURL · 1, rue Gutenberg · B.P. 135 · F-67722 Hoerdt · Tel. +33 (0) 3 88 68 22 68 · Fax +33 (0) 3 88 51 76 88
USA:
MACHEREY-NAGEL Inc. · 6 South Third St., Suite 402 · Easton, PA 18042 · Tel. +1 610 559 9848 · Fax +1 610 559 9878
www.mn-net.com
MN
Ready-to-use Cartridges for Modern Sample Preparation
MACHEREY-NAGEL
www.mn-net.com
MN