SiliaQuick™ QuEChERS
Portmanteau word for Quick–Easy–Cheap–Effective–Rugged–Safe
As simple as 1, 2, 3 !
1.
Liquid Extraction of the compound
of interest : homogenize and centrifuge food
sample to recover compoundsof interest in organic solvent (acetonitrile)
2. Solid phase and Dispersive SPE Clean-up : add
supernatant from Step 1 to a specially designed
dSPE tube and centrifuge,
to remove undesired compounds
(sugars, proteins, lipids, organic acids,
excess water…).
QuEChERS
A highly beneficial sample preparation technique for multi-residue pesticide analysis :
makes it simpler, cheaper and quicker for the
analytical chemist.
Since the publication of the original method in
2003, the QuEChERS
method has gained in popularity
to become the most valuable
alternative in pesticide residue
analysis worldwide. The approach
as spread to multiple organic
compounds, including pesticides,
herbicides, fungicides antibiotics from a vast
array of food matrices.
3. LC or GC Analysis : recover supernatant from step
2 and inject into a LC-MS/MS
or GC-MS instrument.
The QuEChERS procedure
requires a minimal number of
simple steps, thus
ensuring fast results,
low cost and minimal risks.
Traditional Techniques
QuEChERS Techniques
• Multiple steps + Large sample needed
• Pre-packed salt packets and dSPE tubes
to maximize lab throughput
• high solvents consumption
• time consuming procedures
• higher costs, high wastes, erring
• Extremely versatile
• Environmentally-friendly re-usable tubes
• Suitable for a vast array
of complex food matrices
• Significant time reduction approx.
6x faster than traditional SPE method
• Significant cost reduction approx.
9x less solvent than traditional SPE method
• No need of specialized
equipment or glassware
EXTENDED TECHNIQUE –
Schematic Flow Chart of TOP 3 QuEChERS Methods
Meant to orient, but keep in mind all methods can favorably
be optimized regarding your compound to be analyzed
Blend and homogenize the food sample
Weight the sample
STEP 1
Add salts and acetonitrile and shake well
SiliaQuick™ QuEChERS “no
muss, no fuss” Packets
Anhydrous MgSO4 : facilitates solvent partitioning
Acetic acid : for pH adjustment to protect sensitive analytes
Centrifuge for 5 min
Acetonitrile : shows best solvent characteristics for wide pesticides extraction
Buffers : maintain optimal pH and prevent pH degradation
NaCl : limits polar interferences
Add supernatant from
previous extraction to selected dSPE tube
STEP 2
Shake & centrifuge
Purposes of dSPE Sorbents
SiliaMetS Diamine (PSA) : removes sugars, fatty acids, organic acids,
lipids and pigments
SiliaBond C18 : removes long chain, non-polar compounds, and lipids sterols
Inject supernatant for LC or GC analysis
STEP 3
Graphitized Carbon Black (GCB) : removes pigments, polyphenols
and other polar compounds
Anhydrous MgSO4 : removes residual water from the organic phase
MATCHING THE RIGHT FOOD GROUP
WITH THE RIGHT FOOD MATRIX
General
Fruits & Veggies
Pigmented
Fruits & Veggies
Beets, carrots, radishes, patatoes
Highly Pigmented
Fatty Fruits & Veggies
Fatty and Waxed
Fruits & Veggies
-
-
Eggplants, cucumbers, tomatoes
Red pepper, green pepper, pumpkin
-
-
Broccoli, Brussels sprouts, cauliflowers
Broccoli
-
-
Aspargus, celeries, leeks, rhubarb
Rhubarb
-
-
-
Lettuce, parsley, basil, spinach
-
-
Garlic, onions, shallots
-
-
Garlic, onions, shallots
Apples, pears, quinces
-
-
Apricots, cherries, pears, prunes
-
Avocados
Bananas, mangos, pineapples
Mangos
-
-
Strawberries, blackberries,
blueberries, red grapes, cranberries
-
Grapefruit, lemons, limes, oranges,
tangerines
-
Coffee beans, tea leaves
Coffee beans
Cereals
Avocados, bananas
STEP 1, LIQUID EXTRACTION :
The Right SiliaQuick QuEChERS Packet or kit
for the QuEChERS Technique of your Choice
Pre-packed and pre-weighed !
QuEChERS Method
Original
QuEChERS
Buffered
AOAC 2007.01
Buffered
EN 15662
QuEChERS Method
Original
QuEChERS
Buffered
AOAC 2007.01
Buffered
EN 15662
Description
• Cleanest procedure
because of fewer reagents
• No use of acetic acid, sometimes
uncompatible with GC-MS analysis
• 1 % acetic acid in acetonitrile
to maintain optimal pH
• Uses sodium acetate buffer
to prevent pH degradation
• Uses Na2HCitr • 1.5H2O
and Na3Citrate • 2H2O as buffering agent
to preserve sensitive analytes
Description
• Cleanest procedure
because of fewer reagents
• No use of acetic acid, sometimes
uncompatible with GC-MS analysis
• 1 % acetic acid in acetonitrile
to maintain optimal pH
• Uses sodium acetate buffer
to prevent pH degradation
Packet Content
4 g MgSO4
1 g NaCl
6 g MgSO4
1.5 g NaOAc
Product nbr.
for 100 packets
Product nbr.
for kits
(without tubes)
(packets in tubes)
QE-0001-100P
QE-0001-100K
QE-0002-100P
QE-0002-100K
QE-0003-100P
QE-0003-100K
4 g MgSO4
1 g NaCl
0.5 g Na2HCitr•1.5H2O
1 g Na3Citrate•2H2O
Tube Content
4 g MgSO4
1 g NaCl
6 g MgSO4
1.5 g NaOAc
4 g MgSO4
• Uses Na2HCitr • 1.5H2O
1 g NaCl
and Na3Citrate • 2H2O as buffering agent to pres0.5 g Na2HCitr•1.5H2O
erve sensitive analytes
1 g Na3Citrate•2H2O
Product no. for 100 Kits
(with tubes)
QE-0001-100T
QE-0002-100T
QE-0003-100T
STEP 2, SOLID PHASE CLEAN UP :
The Right dSPE Kit for the Right Food Matrix
General
Fruits & Veggies
Tube (mL)
Units/box
2
100
15
Pigmented
Fruits & Veggies
2
15
Highly Pigmented
Fatty Fruits & Veggies
2
15
Fatty and Waxed
Fruits & Veggies
2
15
MgSO4 : Anhydrous Magnesium Sulfate
NaCl : Sodium Chloride
Na3Citrate•2H2O : Trisodium Citrate Dihydrate
Na2HCitr•1.5H20 : Disodium Hydrogencitrate Sesquihydrate
50
MgSO4
PSA
GCB
C18
PRODUCT NO
Method
150
50
-
-
QD-1000-2T
AOAC
150
25
-
-
QD-1001-2T
EN
1200
400
-
-
QD-2000-15T
AOAC
900
150
-
-
QD-2001-15T
EN
150
50
50
-
QD-1002-2T
AOAC
150
25
2.5
-
QD-1003-2T
EN
1200
400
400
-
QD-2002-15T
AOAC
900
150
15
-
QD-2003-15T
EN
150
50
50
50
QD-1004-2T
AOAC
150
25
7.5
-
QD-1005-2T
EN
1200
400
400
400
QD-2004-15T
AOAC
900
150
45
-
QD-2005-15T
EN
150
50
-
50
QD-1006-2T
AOAC
150
25
-
25
QD-1007-2T
EN
1200
400
-
400
QD-2006-15T
AOAC
900
150
-
150
QD-2007-15T
EN
100
50
100
50
100
50
PSA : Primary Secondary Amine - SiliaMetS Diamine
GCB : Graphitized Carbon Black
C18 : C18 Fonctionnalized Silica - SiliaBond C18
TEL. : 1 418 874.0054
FAX : 1 418 874.0355
TOLL-FREE : 1 877.SILICYCLE
(NORTH AMERICA ONLY )
WWW.SILICYCLE.COM
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