2012 Teslin River Chinook Sonar Project CRE-01N-12 Prepared For: The Yukon River Panel Restoration and Enhancement Fund Prepared By: Brian Mercer February 2013 BMA B. Mercer & Associates Ltd. Box 20046 Whitehorse, Yukon Y1A 7A2 ABSTRACT Multiple beam high resolution sonars were used to enumerate the 2012 Chinook salmon (Onchorynchus tshawytscha) escapement to the Teslin River system. This was the first year of a full sonar project at this site following a feasibility study in 2011. The sonar was operated on the mainstem Teslin River at the site identified during the 2011 feasibility study; approximately 12 km upstream of the confluence of the Teslin and Yukon Rivers at Hootalinqua. The camp and sonar station set-up was initiated on July 3. Sonar operation began on July 17 and operated continuously through to September 3. A total of 3,396 targets identified as Chinook salmon was counted during the period of operation. An additional 58 Chinook were estimated to have passed during the 20.5 hours the sonars were inoperative over the course of the project. The total escapement was estimated to be 3,454. The first Chinook salmon was observed on July 27, ten days later than anticipated. A peak daily count of 186 fish occurred on August 21, at which time 76% of the run had passed the sonar station; 90% of the run had passed the station on August 25. A carcass pitch was conducted over approximately 120 km of the mainstem Teslin River, yielding 147 sampled Chinook. Of these, 95 (66%) were female and 52 (34%) were male. The mean fork length of females and males sampled was 853 mm and 773 mm, respectively. The DFO scale lab determined ages from 118 Chinook sampled. Age-5 (68%) was the dominant age class, followed by age-4 fish (28%) and age-3 fish (4%). A total of 106 tissue samples was collected for GSI analysis. i B. Mercer & Associates Ltd. Doc. 7-12 TABLE OF CONTENTS ABSTRACT ......................................................................................................................... i 1. INTRODUCTION ......................................................................................................... 1 Background ..................................................................................................................... 1 Multiple Beam Sonar ...................................................................................................... 2 Study Area ...................................................................................................................... 2 2. OBJECTIVES ................................................................................................................ 5 3. METHODS .................................................................................................................... 5 Site Selection .................................................................................................................. 5 Regulatory Submissions and Permits .............................................................................. 5 Mobilization and Personnel ............................................................................................ 6 Sonar deployment and operation .................................................................................... 6 Target Identification and Species Apportionment ........................................................ 11 Carcass Pitch ................................................................................................................. 11 Hydrometric data and Weather ..................................................................................... 12 Communication ............................................................................................................. 12 4. RESULTS ..................................................................................................................... 12 Chinook Counts ............................................................................................................ 12 Carcass Pitch ................................................................................................................. 15 Above Border Chinook Spawning Escapement Estimate ............................................. 15 5. DISCUSSION AND RECOMMENDATIONS ........................................................... 16 6. REFERENCES ............................................................................................................ 20 Personal Communications ............................................................................................ 22 ACKNOWLEDGEMENTS .............................................................................................. 22 ii B. Mercer & Associates Ltd. Doc. 7-12 LIST OF TABLES Table 1. 2012 Daily counts of Teslin River Chinook salmon.......................................... 14 Table 2. Age, sex, and length summary of sampled and aged 2012 mainstem Teslin Chinook. .................................................................................................................... 16 LIST OF FIGURES Figure 1. Mainstem Teslin River and sonar site. ............................................................... 3 Figure 2. Daily minimum, maximum and mean discharge rates for mainstem Teslin River at station 09AF001 from December 1955 through January 1973. Source: Water Survey Board of Canada. ................................................................................. 4 Figure 3. Aerial view of sonar site depicting the camp, sonar locations and schematic representation of ensonified portions of the river. ...................................................... 7 Figure 4. Sonar unit mounted on stand in deep water......................................................... 8 Figure 5. South Bank sonar configuration and related apparatus, upstream view. Note: Inset illustrates Ampair® model UW100 in-stream water turbine out of the water. .. 9 Figure 6. Camp, NB sonar, and deflection fence ............................................................. 10 Figure 7. Daily Counts of Chinook entering the Teslin River system, 2012. Note: 2011 counts from feasibility study with 12 full days of sonar operation, Aug. 4-15. ....... 13 Figure 8. Cross sectional distribution of migrating Chinook at the Teslin sonar site, 2011 and 2012. ................................................................................................................... 13 Figure 9. Length Frequency histogram of sampled Teslin River Chinook, 2012............ 15 LIST OF APPENDICES Appendix 1. Location of radio tagged Chinook during peak spawning in the Teslin River drainage in 2003. ....................................................................................................... 23 Appendix 2. 2011 Mainstem Teslin River carcass pitch data. ......................................... 24 Appendix 3. Daily count of radio tagged Chinook (n=86) passing the Teslin telemetry station in 2003. .......................................................................................................... 28 Appendix 4. Hydrometric data and weather observations, Teslin sonar site 2012. .......... 29 Appendix 5. Estimated proportions of Big Salmon River and Teslin River Chinook in upper Yukon River Chinook escapements, 2002 through 2012. .............................. 30 Appendix 6. Estimated proportions of Blind Creek, Big Salmon and Teslin River Chinook in upper Yukon River Chinook escapements based on observed radio tag distributions 2002 – 2004.......................................................................................... 30 iii B. Mercer & Associates Ltd. Doc. 7-12 1. INTRODUCTION Background The Yukon River system encompasses a drainage area of approximately 854,000 km2 and contributes to important aboriginal, subsistence and commercial fisheries in the U.S. and Canada. Of the five species of salmon entering the Yukon River, adult Chinook salmon travel the farthest upstream and have been documented at the furthest headwaters of the Teslin system in the McNeil River, 3,300 km from the river mouth (Mercer & Eiler 2004). Approximately 50% of Chinook salmon entering the Yukon River from the Bering Sea is typically destined for spawning grounds in Canada (Eiler et al. 2004, 2006). Canadian origin fish contribute approximately 47% to 67% of the total U.S. commercial and subsistence fisheries (Templin et al. 2005; cited in Daum and Flannery 2009). Canadian and U.S. fishery managers of the Yukon River Joint Technical Committee (JTC) as well as members of the Yukon River Panel (YRP) recognize that obtaining accurate estimates of spawning escapements is required for the management of Yukon River Chinook stocks. The enumeration of genetically distinct stocks, coupled with a representative genetic stock identification (GSI) sampling program can also be used to obtain independent drainage wide above border Chinook escapement estimates1. Quantified Chinook escapements and biological information on the stock is important for post-season run reconstruction, pre-season run forecasts and the establishment of long term biologically based escapement goals. The Teslin River system has been identified, potentially, as a discrete Conservation Unit (CU) under the Fisheries and Oceans Canada (DFO) Wild Salmon Policy (WSP), (DFO 2007). One of the long term goals of the WSP is the establishment of biologically based escapement goals for all species of salmon within designated conservation units. A sufficiently long time series data set of salmon escapements coupled with stock recruitment modelling is the primary recognized method for the establishment of biologically based escapement goals. Currently, there is no in-season monitoring specific to Teslin River Chinook. Teslin River origin Chinook are an important contributor to aboriginal fisheries in the upper Yukon watershed, and are of particular importance to the Teslin Tlingit Council. Monitoring of Teslin River Chinook will assist in achieving long term management and escapement objectives for the Teslin Tlingit Council (TTC). 1 For the purposes of this report border escapement is defined as the number of Chinook salmon estimated to have crossed the Canada/U.S. border into the Upper Yukon River drainage minus the total Canadian harvest. Representative GSI sampling of upper Yukon River Chinook stocks occurs at the Eagle sonar site near the Canada/U.S. border in Alaska. B. Mercer & Associates Ltd. Doc. 7-12 Page 1 Multiple Beam Sonar Fixed-location, side-view sonar techniques are currently the only means of obtaining inseason abundance estimates for anadromous fish stocks in rivers that are too wide for conventional weir structures, too turbid for visual observations and where weir emplacements would be a navigational impediment to water craft. Since 2002, the DIDSON™ high resolution sonar apparatus has been used for enumeration of several species of salmon in a broad range of environments (Galbreath and Barber 2005, Holmes et al. 2006, Maxwell et al. 2004, Enzenhofer et al., 2010). In general, the DIDSON units have been found to be reliable, does not require extensive operator training and provides accurate counts of migrating salmon. The detection and counting of migrating salmon with a properly positioned DIDSON imaging sonar is as accurate as visual counts of fish migrating through an enumeration fence in a clear water river (Holmes et al., 2006). Within the upper Yukon drainage DIDSON sonar has been used on the Big Salmon River (Mercer & Wilson, 2005-2012) and the Klondike River (Mercer, 2009-2011) to enumerate annual Chinook salmon escapements into these systems. The 2012 Teslin sonar project deployed both a standard DIDSON sonar as well as a second generation multiple beam sonar, the ARIS (Adaptive Resolution Imaging Sonar) model. Both models are manufactured by Sound Metrics Corp. The deployment configuration of the sonar is dependent on the characteristics of the particular site, species involved, and the project objectives. Both sonar models produce an ensonified field 29° wide in the horizontal plane and, depending on the transducer lens configuration, from 1° to 12° deep in the vertical plane. The maximum ensonified distance the sonars (with an 8° lens) can detect migrating Chinook is approximately 40 m. With a horizontally aligned 8° transducer lens the maximum ensonifiable depth at 40 m is 5.6 m. For the accurate enumeration of fish it is essential that the sonar is aimed so the beams completely ensonify the area through which fish are migrating. In addition, the bathymetric profile must preclude the presence of acoustic shadows or turbulence (air entrained water) that could mask fish targets. Species identification and/or apportionment should also be unambiguous. Unlike split beam sonar the multiple beam sonar produces a real time image of the target. This imaging capability can allow for the identification of the species based on size, form and swimming characteristics. When mounted in a horizontal configuration the sonar provides information on the distance the target is from the sonar but not the depth of the target within the water column. Study Area The Teslin River system has a drainage area of 36,500 km2 that straddles the Yukon and B.C. border (Figure 1). Teslin Lake is the largest water body in the system with a surface area of approximately 354 km2. Teslin Lake is fed by several drainages in the upper system and discharges at its outlet into the mainstem Teslin River. The lower B. Mercer & Associates Ltd. Doc. 7-12 Page 2 mainstem Teslin River has a mean annual discharge volume of 869 m3/sec (range 1080 – 643 m3/sec; Water survey of Canada, Station 09AF001; Figure 2). The lower mainstem Teslin River area is remote with no highway access. Access to the area is by riverboat from Lake Laberge or Johnson‟s Crossing (approximately 100 and 145 river km respectively) or by floatplane with a direct distance of approximately 95 km from Whitehorse, Yukon. Figure 1. Mainstem Teslin River and sonar site. Digital map data source: Yukon Government spatial data collection. www.geomaticsyukon.ca. B. Mercer & Associates Ltd. Doc. 7-12 Page 3 Fish species documented in the mainstem Teslin River system include, but are likely not limited to, Chinook and Chum salmon (Oncorhynchus tshawytscha and O. keta), Grayling (Thymallus thymallus), Burbot (Lota lota), Inconnu (Stenodus leucichthys), Round Whitefish (Prosopium cylindraceum), slimy sculpin (Cottus cognatus) and longnose sucker (Catostomus catostomus) (DFO FISS database). Figure 2. Daily minimum, maximum and mean discharge rates for mainstem Teslin River at station 09AF0012 from December 1955 through January 1973. Source: Water Survey Board of Canada. A three year radio telemetry study conducted on Yukon River Chinook from 2002 through 2004 indicated that the Teslin River system received on average 19.6% (range 18.1% - 20.8%) of the total Canadian origin Chinook in the Yukon River watershed (Mercer et al. 2004, 2005; Osborne et al. 2003). Based on this radio telemetry data, approximately 70% of the radio tagged Chinook entering the Teslin River watershed were located in the mainstem Teslin River (Appendix 1). The remaining 30% were located throughout several headwater systems draining into Teslin Lake. This proportional distribution within the Teslin system was consistent over the three years of the study (mean 70%, SD = 2). The Chinook stock proportions in other upper Yukon River basin as indicated by the telemetry results have been corroborated by subsequent sonar enumeration projects on the Klondike and Big Salmon rivers (Mercer and Wilson 2005-2011). Genetic stock identification (GSI) data collected at the Eagle sonar site from 2008 through 2011 is also congruent with the stock proportions observed in the three years of telemetry (Unpublished data, DFO Whitehorse). 2 This station, decommissioned in 1973, was located 300 m downstream of the sonar site. B. Mercer & Associates Ltd. Doc. 7-12 Page 4 2. OBJECTIVES The objectives of the 2012 Teslin River sonar project were to: 1. Enumerate the Chinook salmon escapement and obtain information on run timing and diel migration patterns. 2. Conduct spawning ground sampling for age-sex-length data and tissue from postspawn fish. 3. METHODS Site Selection The sonar site used in 2012 was the same as that selected as the best available site during the 2011 feasibility study (Mercer 2012). This site, located approximately 12 km upstream from the confluence with the Yukon River (Figure 1), was initially selected for the following reasons: It is a sufficient distance upstream of the mouth to avoid straying or milling Chinook salmon destined for other headwater spawning sites. The site is in a relatively straight section of the river and far enough downstream from any bends in the river so that recreational boaters using the river have a clear view of the in-stream structures. The river flow is laminar and swift enough to preclude milling or „holding‟ behaviour by migrating fish. Bottom substrates consist of gravel and cobble evenly distributed along the width of the river. The stream bottom profile allows for complete ensonification of the water column by two sonars. The site is accessible by boat and floatplane. Regulatory Submissions and Permits A three year land use permit was granted by the Yukon Territorial Government (YTG) Lands Branch for the sonar camp on the mainstem Teslin River. An application was submitted in 2012 to Transport Canada (Marine Branch), Navigable Waters Protection for approval to install partial fish diversion fences in a navigable waterway. Approval was granted for a potential 2013 sonar operation. The application for a land use permit and the proposed sonar project activities triggered a Yukon Environmental and Socio-economic Assessment (YESSA) review of the project. The in-depth YESSA review of the project and land use application required submissions by the proponent pertaining to: a) The depth of the Teslin River in the area of the proposed project and depth below the river‟s surface the sonar units will be submerged. b) The distance from the OHWM that the sonars will be placed. B. Mercer & Associates Ltd. Doc. 7-12 Page 5 c) How the units and stands with sand bags will be placed in the river and removed seasonally. d) The possible effects or risks on fish and aquatic mammals associated with sonar use. The 2012 and subsequent projects were approved subject to the specified terms and conditions of the YESSA review. Mobilization and Personnel Construction of the camp infrastructure began on July 3 with the transport of materials, construction personnel and project manager to the site via river boat and aircraft. Two tent frames and sanitation facilities were constructed on site. In addition a lean-to was constructed to provide protection for the battery banks, inverters and sonar top boxes. A swiveling frame for the mounting of solar panels was also built (Figure 6). On July 13st, the transport of personnel, sonar equipment, fuel and other required supplies to the sonar site was initiated. The project supervisor was on site continually for the duration of the project, including the carcass pitch. Two BMA technicians were employed on the project on a rotational basis. The project manager was on-site for 10 days during the construction and start-up phase and again at three different times over the course of the project. Sonar deployment and operation Arrangements were made with Sound Metrics Corporation, manufacturer of DIDSON and ARIS sonars, for the rental of a standard range DIDSON sonar unit during the period July 20 through August 20. A second ARIS3 sonar belonging to the contractor was rented for the duration of the project (July 15 through September 3). Due to delivery delays, a DFO owned ARIS sonar unit was loaned to the project from July 15-18. The DFO sonar was a new and untested sonar unit with a beta software version. The DFO unit worked intermittently for 4 days and due to unresolvable software issues was returned to DFO and subsequently to Sound Metrics for repair. The 2012 project operational plan involved emplacement of a sonar unit on each side of the river. The sonar units were configured to aim across the river enabling ensonification of as much of the migration corridor as possible (Figure 3). The ARIS sonar unit was deployed for the full duration of the project (July 19 – September 3) from the north bank of the river. The DIDSON sonar was deployed for 28 days (July 20 - August 19) from the south bank. The results of the two week sonar deployment during the 2011 Teslin sonar feasibility study indicated that 97% of the fish observed passing the site were located within the range of the north bank sonar. The rationale for operating the south bank sonar in 2012 a minimum of four weeks was to verify if the cross sectional distribution of migrating Chinook observed in 2011 would also be observed over the course of the 2012 run. The two sonar units were inter-changed for short periods on July 3 The ARIS and DIDSON sonars are both manufactured by Sound Metrics. The ARIS is considered the “second generation” model of high resolution sonars produced by this company. B. Mercer & Associates Ltd. Doc. 7-12 Page 6 24 and August 6 and the counts cross referenced to ensure they were functioning properly. South Bank sonar North Bank sonar Camp Flow Figure 3. Aerial view of sonar site depicting the camp, sonar locations and schematic representation of ensonified portions of the river. Power for the sonar units, computer apparatus, and wireless transceivers was obtained from 12 volt battery banks located on each side of the river. The 12 volt battery current was converted to 120 volt AC using 1000 watt inverters. The north bank batteries were charged using 4 solar panels as well as a commercial grade battery charger powered by a 2000 watt Honda® generator. The south bank sonar location was in perpetual shade precluding the use of solar panels. These batteries were charged using an Ampair® model UW100 in-stream water turbine (Figure 5), as well as a second Honda® generator and battery charger. The two sonar stands fabricated for the 2011 project (Mercer 2012) were again used in 2012. During the first 14 days of the project, when high water levels precluded installation and use of the prefabricated stands, a different sonar mounting apparatus was used. This consisted of two vertical aluminum pipes driven into the river substrate parallel to the shore. The vertical pipes were connected with a cross pipe on which the sonar was mounted in a similar manner as the prefabricated stand. The tops of the B. Mercer & Associates Ltd. Doc. 7-12 Page 7 vertical pipes were held in place using two adjoining pipes running 90° to the shore (Figure 4). This allowed secure deployment of the sonar apparatus during periods of very high water. However, with the sonar mounting apparatus fixed to the shore, the length of the ensonified fields within the river was also fixed. This resulted in a gap between the ends of the two ensonified fields of approximately 7m - 10m. Figure 4. Sonar unit mounted on stand in deep water. Two 8° concentrator lenses, purchased from Sound Metrics Corporation, were mounted on the standard transducer lens of each sonar unit. The concentrator lens reduces the vertical ensonified field from 14° to 8°. The reduced field size increases the acoustic energy reflected and reduces interference from surface and bottom reverberation as well as overall acoustical “noise”. This results in an increase in the resolution of all target images and, more importantly, increases the resolution and detection of targets in the outer range of the ensonified area where reflected acoustic energy is lowest. Due to the high water levels during the initial period of sonar operation, the concentrator lenses were not used in order to obtain the larger vertical ensonified field. The sonar transducer lenses were positioned to a depth of approximately 12 cm below the surface of the river and angled downward approximately 3º from horizontal. This resulted in the upper edge of the ensonified field of view remaining parallel to the surface of the river. The sonars have an incorporated compass and inclinometer that displays the tilt and azimuth of the sonar beams on the computer display. After the placement of the sonars a diversion fence was positioned 3 m downstream of each sonar unit to deflect migrating Chinook away from the near field of the sonar. The fences consisted of a log boom extending, perpendicular to the current, approximately 6 meters out from the shore (Figures 5 and 6). A 6 m x 2m length of page wire fencing was B. Mercer & Associates Ltd. Doc. 7-12 Page 8 attached to the length of the boom. The bottom margin of the fencing was weighted with rocks so the wire would hang perpendicular to the surface. Wireless data transmitter Sonar unit Deflection boom/fence Water turbine in place Figure 5. South Bank sonar configuration and related apparatus, upstream view. Note: Inset illustrates Ampair® model UW100 in-stream water turbine out of the water. For the DIDSON sonar the receiver gain was set at –40 dB, the window start at 5.0 m, window length at 40 m, and auto frequency was enabled for the duration of the project. With a 40 m window length the default operating frequency is 1.1 Mhz. The recording frame rate was set at 4 frames per second. The ARIS sonar settings were also 4 frames per second with a default frequency of 1.1 Mhz. and receiver gain of 23 dB. The ARIS start and end ranges were 5.0m and 41.5m. The ARIS sonar software requirements necessitated the acquisition of a newer computer. A total of four laptop computers were used for the project. One computer was dedicated for each sonar unit for file acquisition and reading. Since security firewalls on the sonar computers had to be disabled, one computer was dedicated for satellite internet communication. The fourth computer was on hand as a spare. B. Mercer & Associates Ltd. Doc. 7-12 Page 9 Deflection Fence NB sonar Figure 6. Camp, NB sonar, and deflection fence Chinook Enumeration On July 22 the functioning ARIS sonar was in position on the NB. A DIDSON standard sonar, rented from Sound Metrics was deployed on July 19 on the NB and moved to the SB on July 22 with the arrival of the new ARIS unit. The NB sonar was operated continuously over the course of the project with minor planned and unplanned stoppages varying in duration from 15 minutes to 4 hours and 40 minutes. All stoppages were recorded and potentially missed fish were added to the counts by extrapolation based on the mean number of fish per hour recorded the previous 24 hours. During the project the sonar data was collected continuously and stored automatically in pre-programmed, 20 minute date stamped files. This resulted in an accumulation of 72 files over a 24 hour period for each unit. These files were subsequently reviewed the following day and backed up on an external hard drive. To optimize target detection during file review, the background subtraction feature was used to remove the static river bottom structure. The intensity (brightness) was set at 40 dB and threshold (sensitivity) at 3dB. The playback speed depended on the preference and experience of the observer, but was generally set between 40 and 50 frames per second, approximately 10 - 12 times the recording rate. When necessary, the file review was stopped when a target was observed and replayed at a slower rate to aid in identifying passing fish. All targets identified as Chinook were visually counted and B. Mercer & Associates Ltd. Doc. 7-12 Page 10 entered into an excel spreadsheet. The position of each Chinook observed within the cross section profile of the river was recorded in 5 m increments. This provided information on the spatial pattern of migrating Chinook within the ensonified area of the river. A record was maintained of each 20 minute file count as well as hourly, daily and cumulative counts. The count data obtained from each sonar was combined and entered daily into a master spreadsheet. Target Identification and Species Apportionment Fish identification and species apportionment was based on size, form and swimming behaviour of detected sonar targets. The minimum size used to classify Chinook salmon for the project was 50 cm. The target measurement feature of the DIDSON software was used to estimate the size of the observed fish when required. With adequate training it has been demonstrated that DIDSON sonar operators are able to differentiate, with a high degree of accuracy, between resident fish species and migrating salmon (Enzenhofer et al. 2010). Blind trials comparing both visual and DIDSON sonar counts of Chinook on the Klondike River demonstrated that trained sonar operators detected and correctly identified 100% of the visually observed (n=106) Chinook passing the Station (Mercer 2010). Co-migrating Chum salmon could be incorrectly identified as migrating Chinook due to similar form, size and swimming behaviour. Fall Chum salmon are documented spawning in the mainstem Teslin River (DFO FISS database). However, it is unlikely a temporal overlap of migrating fall Chum and Chinook occurs in the Teslin system. Test fishing at the Eagle sonar station from 2007 through 2009 captured the first fall Chum Salmon on August 8, 9, and 19 respectively (Crane and Dunbar, 2009 and 2011). The first Chum salmon counted in 2012 at the Eagle sonar sites was on August 23. (http://www.adfg.alaska. gov/sf/FishCounts/index.cfm?) The river distance from Eagle Alaska to the Teslin sonar site is approximately 780 km. Mean migration rates for upper Yukon River fall Chum have been documented at approximately 40 km/day (Boyce 1999). This suggests the Teslin Chinook migration would have been complete before the arrival of fall Chum in the system. Carcass Pitch The carcass pitch on the upper mainstem Teslin River was conducted by a crew of two technicians. Access to Chinook spawning areas was by a river boat powered by a 60 hp outboard jet. The crew made two trips during the periods August 31 – September 2 and September 7 – 10. The crew accessed the river from the sonar site to a point approximately 100 km upstream on the first trip. For the second trip the river was accessed at the Johnson‟s Crossing boat ramp, located approximately 4 km downstream from the outlet of Teslin Lake (Figure 1). The second trip extended downstream approximately 110 km from Johnson‟s Crossing. Only dead or moribund fish were collected. Collection was by hand and an extendable spear. Tissue samples for DNA analysis were collected from fresh (gills still red) carcasses only. A portion of both the left and right axillary appendages was removed using B. Mercer & Associates Ltd. Doc. 7-12 Page 11 guillotine clippers and placed in paired vials containing 95% ethyl alcohol4. Sampling for age, sex and length data was conducted on all moribund and dead Chinook located. Five scales were obtained from all recovered fish for aging. The sex and mid-eye-fork and post-orbital hypural lengths (to the nearest 5 mm) were also recorded for each recovered fish. Tissue samples, scale cards and an electronic copy of the ASL data were submitted to DFO Whitehorse on completion of the project. Hydrometric Data and Weather Air and water temperatures and levels were recorded daily at the sonar site. A referenced water gauge was established so that relative water levels can be recorded in subsequent years. Anecdotal weather observations were also recorded. Communication For safety purposes a satellite phone was present in the camp at all times as well as carried during extended boat trips. In addition, a satellite internet system was installed at the site. The satellite internet system facilitated communications between the field personnel, the project manager and the technical support team from Sound Metrics. Biweekly sonar counts were passed on to DFO Whitehorse, the TTC Department of Lands and Resources and other interested parties using email. The satellite internet proved to be an important aid in resolving sonar hardware and software problems. The satellite internet allowed direct real time communication and file sharing with technical staff at Sound Metrics. 4. RESULTS Chinook Counts A total of 3,396 targets identified as Chinook salmon was counted during the period of operation. Due to planned and unplanned stoppages in the sonar, a total of 20.5 hours of sonar data collection was missed. Extrapolating the counts during the down time resulted in the addition of 58 (1.7% of count) Chinook to the counted total. The estimated total Chinook escapement count including the extrapolation is 3,454. The first Chinook salmon was observed on July 27, 7 to 10 days later than anticipated. A peak daily count of 186 fish occurred on August 21, at which time 76% of the run had passed the sonar station; 90% of the run had passed the station on August 25. Daily and cumulative counts are presented in Table 1 and illustrated in Figure 7. The cross sectional distribution pattern of the migrating Chinook is presented in Figure 8. The distribution of Chinook passing the station was wholly skewed to the north side of the river with no Chinook detected by the SB sonar. This pattern is similar to that 4 One of each pair of samples is sent to both the Pacific Biological Station in Canada and the Alaska Department of Fish & Game (ADF&G) Gene Conservation Laboratory for analysis. B. Mercer & Associates Ltd. Doc. 7-12 Page 12 observed in 2011 when 97% of observed Chinook were detected with the NB sonar. There were more fish observed in the 5-10 m range in 2012 than in 2011. This was likely due to the presence of the deflection fence downstream of the NB sonar in 2012. This fence was absent during the 2011 feasibility study and therefore fish within the near field of the sonar (<5m) would have been missed. Figure 7. Daily Counts of Chinook entering the Teslin River system, 2012. Note: 2011 counts from feasibility study with 12 full days of sonar operation, Aug. 4-15. Figure 8. Cross sectional distribution of migrating Chinook at the Teslin sonar site, 2011 and 2012. B. Mercer & Associates Ltd. Doc. 7-12 Page 13 Table 1. 2012 Daily counts of Teslin River Chinook salmon. B. Mercer & Associates Ltd. Doc. 7-12 Page 14 Carcass Pitch A total of 146 dead or moribund Chinook was recovered during the carcass pitches. The majority of these (141) were recovered during the second carcass pitch trip on Sept 7 -9. The low recovery in the first trip was likely due to delayed spawning as a result of the lateness of the run. Of the fish collected, 94 (64%) were female and 52 (36%) were male. The mean mid-eye fork length of females and males sampled was 853 mm and 773 mm, respectively. Complete age data5 was determined from 118 of the Chinook sampled; the remaining 28 samples yielded partial ages or no ages due to regenerate scales. Age-5 (1.3) (68%) was the dominant age class, followed by age-4 fish (28%) and age-3 fish (4%). A total of 106 tissue samples was collected for GSI analysis. Average length at age data for male and female Chinook sampled are presented in Table 2 and Figure 9. Complete age, length and sex data are presented in Appendix 2. Figure 9. Length Frequency histogram of sampled Teslin River Chinook, 2012. Above Border Chinook Spawning Escapement Estimate The 2012 Eagle sonar project on the Yukon River downstream of the Canada/U.S. border yielded a Chinook estimate of 34,747 and a spawning escapement6 estimate of 32,658 Chinook salmon (DFO Whitehorse unpublished data 2013). Genetic stock identification (GSI) samples were also obtained at this site using drift nets. The GSI data provides information on the proportional contribution of identified stocks to the total above border Chinook escapement. The 2012 proportional contribution of the regional Teslin River stock to the Chinook border escapement based on non-weighted analysis of the GSI 5 Scale age analysis was conducted for DFO Whitehorse by the Pacific Biological Station, fish ageing lab in Nanaimo, British Columbia. 6 Spawning escapement is the Eagle sonar count minus the catches in the U.S. above the sonar station and in the Canadian fisheries. B. Mercer & Associates Ltd. Doc. 7-12 Page 15 samples was 37.8% (SD 4.6), (DFO Whitehorse unpublished data). Using the 2012 Teslin sonar count and the proportion of Teslin origin stock derived from the Eagle GSI sampling, the 2012 expanded Chinook border escapement estimate would be 9,138 (95% CI, 7,380-12,035). This is significantly lower than the escapement derived from the Eagle sonar estimate. This incongruity is examined in the discussion section below. Table 2. Age, sex, and length summary of sampled and aged 2012 mainstem Teslin Chinook. SEX F Age 1.2 1.3 1.4 Average of MEF (mm) Count of Age M 1.2 1.3 1.4 Data Average of MEF (mm) Count of Age Average of MEF (mm) Count of Age Average of MEF (mm) Count of Age Average of MEF (mm) Count of Age Average of MEF (mm) Count of Age Average of MEF (mm) Count of Age M Average of MEF (mm) M Count of Age Total Average of MEF (mm) Total Count of Age Total 770 2 855 14 854 60 853 76 800 3 762 19 765 20 766 % 42 821 35.6% 118 100.0% 1.7% 11.9% 50.8% 64.4% 2.5% 16.1% 16.9% 5. DISCUSSION AND RECOMMENDATIONS In 2012 the water levels throughout the Yukon drainage were significantly higher than average when Chinook began entering the Teslin River system ((USGC 2012; Appendix 4). The record high flows in 2012 were likely the reason the Chinook run was 7-10 days later than average in the upper Yukon River. The first Chinook observed passing the Teslin sonar site in 2012 was 11 days later than the first radio tagged fish was recorded entering the system in 2003 (Appendix 3). The high water levels at the Teslin sonar site in 2012 necessitated mounting the sonar units close to the shore. This resulted in a 10 meter gap in the center of the river between B. Mercer & Associates Ltd. Doc. 7-12 Page 16 the ensonified fields during the first half of the project. Over the course of the project this gap decreased as the lower water levels allowed the sonars to be moved further out from the shore. The distribution of the fish as detected by the sonars suggests that all passing Chinook were within the ensonified field of the NB sonar. As the NB sonar unit was moved closer to the center of the river over the course of the project the ratio of Chinook detected in the 40-45m range did not increase, suggesting the observed distribution was correct. During the 2011 Teslin sonar feasibility study, due the lower water levels, there was not a gap in the sonar coverage. The Chinook distribution observed in 2011 indicated few Chinook migrated in the center of the river at the sonar site during the study (Mercer 2012). If the Teslin sonar project continues it is suggested the design of the sonar stands and diversion fences be changed. A fixed diversion fence such as that employed on the Big Salmon sonar project, and extending 10m from each shoreline would allow more latitude in the positioning of the sonar units (Mercer and Wilson 2011). As well, this type of fence would provide a higher degree of certainty that all passing Chinook are diverted out from the shore and into the ensonified field. Larger and more robust sonar stands would allow the sonars to be deployed at least 5m from each bank during periods of high water. This would eliminate the non-ensonified space in the center of the river. The 2012 above border Yukon River Chinook escapement estimate based on the Teslin sonar count and the GSI derived stock proportions (9,138; 95% CI +/- 2,987) are significantly incongruent with the escapement estimate obtained from the Eagle sonar count of 32,656. The Teslin sonar derived above border escapement is also not concordant with the above border estimate (38,104, CI 20,926 – 210,9927) obtained from expansion of the 2012 Big Salmon River sonar counts (Mercer and Wilson 2013). It is not within the scope of this report to examine all the possible causes of these discrepancies. However, the value of accurate escapement data is increased if it can be correlated with accurate stock composition information. An accurate count of a Chinook stock or aggregate stocks coupled with accurate GSI stock proportions has the capability of generating independent8 upper Yukon Chinook escapement estimates that are within defined statistical parameters. It is recognized that the collection and application of Yukon Chinook GSI data as a method of population specific estimates of stock composition is an emerging technique in development. As additions and refinements are made to the GSI database and sampling protocol the value and accuracy of the GSI derived stock composition estimates will increase. The following are some observations on the upper Yukon River Chinook GSI sampling and analysis that may relate to the discordant 2012 data sets: 1. The 2012 GSI sample size of 344 obtained from the Eagle project is considerably below the target sample size of 900 and also below the previously obtained sample sizes (2008-2011 mean = 585, range 453-919). 7 The relatively small Big Salmon GSI stock proportion of 6.7% (SD 2.8) results in a wide margin of error for the upper Yukon Chinook escapement estimate derived from expansion of the Big Salmon sonar count (Mercer and Wilson 2013). 8 Using data separate from the Eagle or Pilot Station sonar projects. B. Mercer & Associates Ltd. Doc. 7-12 Page 17 2. The 2012 Teslin and Big Salmon GSI stock proportions obtained from sampling at Eagle are both outside the ranges previously observed in 3 years of radio telemetry data (2002-2004) and 4 years of sampling at Eagle from 2008 through 2011 (Appendix 5). 3. The 2012 analyzed GSI samples from Eagle were not weighted by run abundance. 4. It is not known if the GSI stock proportions from the Eagle sampling effort are representative of the population. The GSI stock proportions derived from Eagle sampling and the DFO operated fish wheels in 2008 were significantly different (unpublished data, DFO Whitehorse). This suggests different stocks may have different migration patterns within the river depending on water levels and bank orientation. 5. The upper Yukon Chinook baseline GSI database is incomplete and in some cases baseline stocks are mis-identified or are composed of an aggregate of sub-stocks. It is probable that this contributes to biases that are not currently quantifiable. If some stocks are biased high in a given year it follows that others will be biased low. In addition to the Teslin sonar project there were two other escapement monitoring projects in the upper Yukon watershed in 2012; the Blind Creek weir and the Big Salmon sonar projects. The 2012 Blind Creek weir project counted 157 Chinook, the lowest count in 10 years of operation (2003 -2011 mean = 564; range 270-1,155). The 2012 Big Salmon sonar project counted 2,553 Chinook, the second lowest count in 8 years of operation (2005-2011 mean = 5,272; range 1,329 – 9,261). Although there were only 12 days of complete counts from the 2011 Teslin project, comparisons between the 2011 and 2012 Teslin sonar counts indicate the 2011 counts were approximately double the 2012 counts (Figure 7), with a maximum count at the peak of the run of 14.3 fish/hour versus 7.7 fish/hour (Mercer 2012). These three indices suggest the 2012 upper Yukon Chinook escapement was considerably below average. With the discrepancies observed with the 2012 GSI stock compositions it may be instructive to examine the 2012 escapement data using other available information on upper Yukon Chinook stock proportions. The 2002 through 2004 Yukon River telemetry program yielded stock proportions of Chinook in upper Yukon River tributaries based on observed radio tag distributions. The radio tagged fish were tracked into all the terminal areas and 95% of all radio tagged fish entering the upper Yukon watershed were accounted for either in fisheries or in terminal spawning areas. It should also be noted that the inter-annual variance9 of the relative stock proportions within all the larger tributaries was low and consistent over the 3 years of the telemetry program (J. Eiler per. Comm.). The telemetry derived stock proportions for Blind Creek and the Big Salmon and Teslin Rivers are presented in Appendix 6. Using the combined 2012 counts (6,186) from these three projects and the mean sum of the telemetry derived stock proportions 9 Between-year differences observed during 2002-2004 were analyzed using a multivariate test for proportions. No significant differences were detected within the groups (SSB = 0.0048, p = 0.80), suggesting that the stock compositions were comparable during the three years of the study. A similar pattern occurred within regional areas, with equivalent composition estimates observed for most of the larger individual stocks. Only the Little Salmon River showed significant inter-annual differences based on 95% confidence intervals, with a higher proportion of the return to this tributary in 2003 than in the other two years of the study. B. Mercer & Associates Ltd. Doc. 7-12 Page 18 (32.8%, SD 1.6) yields a 2012 upper Yukon Chinook escapement estimate of 18,860 (95% CI 17,231- 20,899). This estimate is significantly below the escapement estimate of 32,656 derived from the Eagle sonar count. It is understood the telemetry derived stock proportions may not be the same as the 2012 stock proportions. However, it may be informative to use the historical telemetry stock proportions, as well as current GSI information, until the problems with sampling error and the incomplete baseline associated with the GSI data are satisfactorily resolved. In summary, the 2012 Teslin sonar project demonstrated that it is a viable means of obtaining accurate escapement counts as well as age, sex and length data of Chinook salmon returning to the Teslin River system. With refinements to the fish deflection fences and continued use of two sonars for at least 3-4 weeks over the course of the run it will be possible to obtain accurate counts of future Chinook escapements into this system. B. Mercer & Associates Ltd. Doc. 7-12 Page 19 6. REFERENCES Boyce, Ian. 1999. Upper Yukon Radio Telemetry Tracking Station Installation and Spawning Ground Sampling/Tag recovery, 1998. R&E Project 09-98-Part 1. Unpublished report for CRE project 09-98, Yukon River Panel. Crane, A.B., and R. D. Dunbar. 2011 Sonar estimation of Chinook and fall Chum salmon passage in the Yukon River near Eagle, Alaska, 2009. Alaska Department of Fish and Game, Fishery Data Series No. 11-08, Anchorage. Crane, A.B., and R. D. Dunbar. 2009 Sonar estimation of Chinook and fall Chum salmon passage in the Yukon River near Eagle, Alaska, 2007. Alaska Department of Fish and Game, Fishery Data Series No. 09-30, Anchorage. Daum, D.W. and B.G. Flannery. 2009. Canadian origin Chinook salmon rearing in nonnatal U.S. tributary streams of the Yukon River, Alaska, 2006-2007. Alaska Fisheries Technical Report No. 102, May 2009. Department of Fisheries and Oceans, Salmon and Freshwater Ecosystems Division, Science Branch. 2007. Conservation Units for Pacific salmon under the Wild Salmon Policy. Conservation Units for Pacific salmon under the Wild Salmon Policy. CSAS Research Document 2007/070. Eiler, J.H., T.R. Spencer, J.J. Pella, and M.M. Masuda, and R.R. Holder. 2004. Distribution and movement patterns of Chinook salmon returning to the Yukon River Basin in 2000 – 2002. U.S. Dept. of Commerce, NOAA Technical Memorandum NMFS-AFSC-148. Eiler, J.H., T.R. Spencer, J.J. Pella, and M.M. Masuda. 2006. Stock composition, run timing, and movement patterns of Chinook salmon returning to the Yukon River Basin 2004. U.S. Dept. of Commerce, NOAA Technical Memorandum NMFS-AFSC-165. Enzenhofer, H.J., Cronkite, G.M.W., and Holmes, J.A. 2010. Application of DIDSON imaging sonar at Qualark Creek on the Fraser River for Enumeration of adult pacific salmon: An operational manual. Can. Tech. Rep. Fish. Aquat. Sci. 2869: iv + 37 p. FISS database, DFO Yukon/Transboundary Rivers area. http://habitat.rhq.pac.dfompo.gc.c./fiss/dcf01.cfm Galbreath, P.F. and P.E. Barber. Validation of Long-Range Dual Frequency Identification Sonar for Fish Passage Enumeration in the Methow River. Unpublished report for the PSC Southern Fund project. 2005. B. Mercer & Associates Ltd. Doc. 7-12 Page 20 Holmes, J. A., Cronkite, G. M. W., Enzenhofer, H. J., and Mulligan, T. J. 2006. Accuracy and precision of fish-count data from a „„dual-frequency identification sonar‟‟ (DIDSON) imaging system. ICES Journal of Marine Science, 63: 543e555. Joint Technical Committee (JTC) of the Yukon River US/Canada Panel. 2010. Yukon River Salmon 2010 Season Summary and 2011 Season Outlook. Fisheries and Oceans Canada, Stock Assessment and Fisheries Management Section, Yukon and Transboundary Area. Whitehorse, Yukon Territory. Maxwell, S., D. Burwen, and C. Pfisterer. Testing the Range Limitations of the Long Range and Standard Versions of the Dual Frequency Identification Sonar (DIDSON). Draft report. Regional Information Report No. 2A04-XX April 2004. Mercer, B. 2005. Distribution and Abundance of Radio Tagged Chinook Salmon in the Canadian Portion of the Yukon River Watershed as Determined by 2004 Aerial Telemetry Surveys. CRE project 77-04, Yukon River Panel. Mercer, B. and J Eiler, 2004. Distribution and Abundance of Radio Tagged Chinook Salmon in the Canadian Portion of the Yukon River Watershed as Determined by 2003 Aerial Telemetry Surveys. Unpublished report for CRE project 77-03, Yukon River Panel. Mercer, B. 2012. 2011 Teslin River DIDSON Sonar Feasibility Study. Unpublished report for the Yukon River Panel, CRE-01N-11. Mercer, B. 2009 through 2011. Klondike River DIDSON Sonar Feasibility Study Unpublished report for Yukon River Panel, CRE projects 16-08 through 16-11. Mercer, B. and J. Wilson, 2005 through 2012. Chinook salmon Sonar Enumeration on the Big Salmon River. Unpublished reports for Yukon River Panel CRE project 41. Water Survey of Canada. Archived hydrometric data. http://www.wsc.ec.gc.ca/applications/H2O/ reporteng.cfm?station=09AF001 from December 1955 through January 1973. Osborne, C.T., B. Mercer, and J.H. Eiler, 2003. Radio Telemetry Tracking of Chinook Salmon in the Canadian Portion of the Yukon River Watershed – 2002. CRE project 78-02, Yukon River Panel. United States Geological Survey. Water Information System. http://nwis.waterdata.usgs.gov/usa/nwis/uv/?cb_00060=on&cb_00065=on&format= gif_stats&period=&begin_date=2012-01-01&end_date=2012-1230&site_no=15356000 B. Mercer & Associates Ltd. Doc. 7-12 Page 21 Personal Communications 2013. John Eiler. Fishery Research Biologist. NOAA, National Marine Fisheries Service, Auke Bay Laboratories, Juneau, AK. 2013. Wade Hanna. Water Survey of Canada. Environment Canada, Whitehorse, Yukon. ACKNOWLEDGEMENTS Several individuals contributed to the 2012 Teslin River sonar project. Marcus Leijon assisted with the camp construction and delivery of materials to the site. David Macdonald, Al Macleod and John Bylenga assisted with the camp setup, conducted the sonar operations and the carcass pitch. Jane Wilson assisted with logistics and expediting and provided editorial support for this report. Elizabeth Macdonald of DFO Whitehorse provided GSI data and other technical input. Funding for this project was provided by the Yukon River Panel Restoration and Enhancement Fund. . B. Mercer & Associates Ltd. Doc. 7-12 Page 22 Appendix 1. Location of radio tagged Chinook during peak spawning in the Teslin River drainage in 2003. Source: Mercer and Eiler, 2004. Sonar Site B. Mercer & Associates Ltd. Doc. 7-12 Page 23 Appendix 2. 2011 Mainstem Teslin River carcass pitch data. DATE 31-Aug 31-Aug 31-Aug 1-Sep 1-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep 7-Sep FISH no. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 SEX F F F F M F F F F F F F F F F F F F F F F F F F F F F M M F F F F F F F F F F M F M F F MEF (mm) 930 785 840 810 800 840 875 835 840 875 835 885 860 POHL (mm) 820 685 750 710 695 740 790 725 730 770 725 720 790 780 670 720 770 770 745 775 760 710 770 660 790 750 790 670 750 770 760 735 785 755 900 905 890 845 735 860 685 860 860 795 810 795 750 640 750 590 765 770 880 870 825 870 890 835 870 810 865 750 890 850 890 785 850 875 860 B. Mercer & Associates Ltd. Doc. 7-12 SCALE BOOK no. 82338 82338 82338 82338 82338 82338 82338 82338 82338 82338 82339 82339 82339 82339 82339 82339 82339 82339 82339 82339 82340 82340 82340 82340 82340 82340 82340 82340 82340 82340 82341 82341 82341 82341 82341 82341 82341 82341 82341 82341 82342 82342 82342 82342 BOOK no. 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 3 3 4 4 4 4 4 4 4 4 4 4 5 5 5 5 SCALE no. 1-41 2-42 3-43 4-44 5-45 6-46 7-47 8-48 9-49 10-50 1-41 2-42 3-43 4-44 5-45 6-46 7-47 8-48 9-49 10-50 1-41 2-42 3-43 4-44 5-45 6-46 7-47 8-48 9-49 10-50 1-41 2-42 3-43 4-44 5-45 6-46 7-47 8-48 9-49 10-50 1-41 2-42 3-43 4-44 Age 14 14 14 14 14 14 14 14 14 14 14 13 M3 14 14 14 14 14 M4 M3 14 13 14 13 13 14 14 14 14 14 14 14 14 14 13 14 13 14 14 M4 Page 24 DATE 7-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep FISH no. 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 SEX F F F F F F F F F M M M M F F M F M F F F M M F M M M M M M M M F M M M M F F F F F F F F F MEF (mm) 860 735 895 905 940 895 830 845 855 790 720 930 935 830 850 895 920 835 770 750 905 890 700 770 680 720 750 760 690 775 870 740 835 650 720 850 825 795 865 770 890 865 830 845 POHL (mm) 750 650 790 810 835 785 740 750 750 685 630 810 820 740 760 660 790 800 740 730 690 650 790 800 600 680 600 630 650 665 600 670 765 630 730 540 630 760 730 705 765 675 785 755 725 745 B. Mercer & Associates Ltd. Doc. 7-12 SCALE BOOK no. 82342 82342 82342 82342 82342 82342 82343 82343 82343 82343 82343 82343 82343 82343 82343 82343 82344 82344 82344 82344 82344 82344 82344 82344 82344 82344 82345 82345 82345 82345 82345 82345 82345 82345 82345 82345 82346 82346 82346 82346 82346 82346 82346 82346 82346 82346 BOOK no. 5 5 5 5 5 5 6 6 6 6 6 6 6 6 6 6 7 7 7 7 7 7 7 7 7 7 8 8 8 8 8 8 8 8 8 8 9 9 9 9 9 9 9 9 9 9 SCALE no. 5-45 6-46 7-47 8-48 9-49 10-50 1-41 2-42 3-43 4-44 5-45 6-46 7-47 8-48 9-49 10-50 1-41 2-42 3-43 4-44 5-45 6-46 7-47 8-48 9-49 10-50 1-41 2-42 3-43 4-44 5-45 6-46 7-47 8-48 9-49 10-50 1-41 2-42 3-43 4-44 5-45 6-46 7-47 8-48 9-49 10-50 Age 14 14 14 M4 14 M4 M4 14 1F 13 14 13 14 13 14 14 M4 14 13 12 14 14 13 13 13 13 13 13 14 14 13 14 13 13 M4 14 14 14 M3 14 M4 14 14 14 Page 25 DATE 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 8-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep FISH no. 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 SEX F F F F M F F F F M M F F F F M M M M F F M M F M F F M M M M M F F M M F F M M F M M M M M MEF (mm) 825 860 820 860 910 865 850 805 530 710 900 875 835 890 730 970 690 635 795 860 820 540 810 920 850 870 880 730 830 820 720 720 810 850 885 910 735 745 730 POHL (mm) 735 765 720 770 815 760 755 710 810 455 615 810 775 735 775 630 840 590 560 700 780 730 470 720 820 750 780 760 640 710 445 720 715 630 630 730 710 730 780 705 750 790 640 650 630 B. 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Doc. 7-12 SCALE BOOK no. 82347 82347 82347 82347 82347 82347 82347 82347 82347 82347 82348 82348 82348 82348 82348 82348 82348 82348 82348 82348 82349 82349 82349 82349 82349 82349 82349 82349 82349 82349 82350 82350 82350 82350 82350 82350 82350 82350 82350 82350 95359 95359 95359 95359 95359 95359 BOOK no. 10 10 10 10 10 10 10 10 10 10 11 11 11 11 11 11 11 11 11 11 12 12 12 12 12 12 12 12 12 12 13 13 13 13 13 13 13 13 13 13 14 14 14 14 14 14 SCALE no. 1-41 2-42 3-43 4-44 5-45 6-46 7-47 8-48 9-49 10-50 1-41 2-42 3-43 4-44 5-45 6-46 7-47 8-48 9-49 10-50 1-41 2-42 3-43 4-44 5-45 6-46 7-47 8-48 9-49 10-50 1-41 2-42 3-43 4-44 5-45 6-46 7-47 8-48 9-49 10-50 1-41 2-42 3-43 4-44 5-45 6-46 Age 14 14 14 14 M4 14 14 12 13 14 14 M4 14 14 14 13 1F 14 14 M4 12 13 14 14 13 14 1F 13 12 1F 13 14 13 M3 14 14 13 14 14 14 14 13 13 13 M4 Page 26 DATE 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep 9-Sep FISH no. 137 138 139 140 141 142 143 144 145 146 147 SEX F F M F M M F F F F F MEF (mm) 825 790 760 960 750 POHL (mm) 720 680 670 840 660 930 820 820 910 850 820 715 730 815 740 B. Mercer & Associates Ltd. Doc. 7-12 SCALE BOOK no. 95359 95359 95359 95359 95360 95360 95360 95360 95360 95360 95360 BOOK no. 14 14 14 14 15 15 15 15 15 15 15 SCALE no. 7-47 8-48 9-49 10-50 1-41 2-42 3-43 4-44 5-45 6-46 7-47 Age 13 13 14 13 12 14 14 14 M4 14 Page 27 Appendix 3. Daily count of radio tagged Chinook (n=86) passing the Teslin telemetry station in 2003. Note: Black line is a 5 day moving average; Source: Mercer and Eiler , 2003 B. Mercer & Associates Ltd. Doc. 7-12 Page 28 Appendix 4. Hydrometric data and weather observations, Teslin sonar site 2012. Date 18-Jul 19-Jul 20-Jul 21-Jul 22-Jul 23-Jul 24-Jul 25-Jul 26-Jul Time 0600 0800 0800 0800 0800 0800 0800 0800 0800 Air Temp. C 15.0 15.0 10.0 Water Temp. C 10.0 9.5 10.0 10.0 13.0 13.0 12.0 13.0 12.0 Water Level cm n/a n/a n/a n/a n/a n/a 148.59 142.24 134.62 27-Jul 28-Jul 29-Jul 30-Jul 31-Jul 0800 0800 0800 0800 0800 15.0 16.0 13.0 8.0 13.0 14.0 12.0 11.0 11.0 11.0 130.81 124.46 122.555 11.43 111.125 01-Aug 02-Aug 03-Aug 04-Aug 05-Aug 06-Aug 0800 0800 0800 0800 0800 0800 14.0 14.0 14.0 6.0 8.0 11.0 11.0 11.0 11.0 10.5 11.0 10.5 108.585 102.87 99.06 94.615 90.17 86.36 07-Aug 08-Aug 09-Aug 10-Aug 11-Aug 12-Aug 0800 0830 0830 0830 0830 0930 15.0 16.0 14.0 9.0 16.0 14.0 11.5 12.0 12.0 11.0 11.0 12.0 82.55 77.47 74.93 74.93 72.39 69.85 13-Aug 14-Aug 15-Aug 16-Aug 17-Aug 18-Aug 19-Aug 20-Aug 0830 0830 0830 0830 0830 11.0 9.0 13.0 12.0 10.0 11.0 12.0 12.0 12.0 12.0 0830 12.0 11.5 67.31 64.77 62.23 59.69 57.15 54.61 52.07 49.53 21-Aug 22-Aug 23-Aug 24-Aug 25-Aug 26-Aug 27-Aug 28-Aug 29-Aug 30-Aug 31-Aug 01-Sep 02-Sep 03-Sep 04-Sep 0830 0830 0830 0830 0930 0830 0830h 0930h 0800h 0800h 0800h 0800h 0800h 0800h 13.0 12.0 14.0 16.0 13.0 7.0 9.0 8.5 3.0 3.0 13.5 13/0 10.0 5.0 11.0 12.0 12.0 11.0 11.0 11.0 11.0 10.5 7.0 9.0 9.0 8.0 10.0 7.0 B. Mercer & Associates Ltd. Doc. 7-12 49.53 44.45 44.45 41.91 39.37 36.83 34.29 34.29 31.75 31.75 26.67 26.67 25.4 25.4 25.4 Comments Water level refference point is 94 inches 238.76cm water level checked at 1600h, Hot Hot day, mostly clear few clouds in evening water level checked at 1300h, Bright hot day with a few clouds Windy, low pressure zone arrives. Cool and cloudy. Mixed Weather. A few showers, some sunny time. Dramatic skies Mixed Weather. Clearer morning. Increasing clouds Cloudy, Windy. Cooler. Sunny some clouds Overcast and rainy New post put in 50'' is equal to 35.5'' on the old. 108.5'' to primary refference point. Recordings from here on in are on the new post. Warm and sunny, clouding over in the afternoon Cloudy, Rain Overcast Blue sky, sunny Patchy clouds, rain overnight, rain in afternoon, New post put in 58" is equal to 42" on previous Overcast and rainy heavy fog, burning off to blue sky and sunny Blue sky, sunny Blue sky, sunny Cool, overcast Heavy rain opening up to sun, mixed cloud and a clear night Mixed sun and cloud Sunny Days…. 50inches on the old is equal to 37inches on the new Blue sky, sunny mild, low cloud cover cloudy, rain overnight blue sky, sunny and breezy low clouds, rainy foggy, cool morning Page 29 Appendix 5. Estimated proportions of Big Salmon River and Teslin River Chinook in upper Yukon River Chinook escapements, 2002 through 2012. Year 2002 2003 2004 2006 2007 2008 2009 2010 Method Telemetry Telemetry Telemetry Fishwheel GSI Sampling Fishwheel GSI Sampling Fishwheel GSI Sampling Eagle Gillnet GSI Sampling Eagle Gillnet GSI Sampling Big Salmon River Estimated % proportion of border escapement based on telemetry or GSI sampling 9.2 15.1 10.0 10.7 (3.2) 10.6 (2.5) 9.3 (6.0) 16.9 (3.6) 11.7 (2.4) 2011 Eagle Gillnet GSI Sampling 8.0 (2.4) 16.4(3.4) 25.6(3.3) 33.0(4.6) 25.3(3.3) 2012 Eagle Gillnet GSI Sampling Mean 20022011 Std. Dev. 6.7 (2.8) 37.8 (4.6) 11.3 2.4 19.6 1.1 Teslin River Estimated % proportion of border escapement based on telemetry or GSI sampling 19.8 18.1 20.8 14.2 (2.4) 9.6 (1.6) Data source: DFO unpublished data, Osborne et al. 2003, Mercer and Eiler 2004, Mercer 2005. Note: Updating of GSI Baseline data may change the GSI based proportions. Appendix 6. Estimated proportions of Blind Creek, Big Salmon and Teslin River Chinook in upper Yukon River Chinook escapements based on observed radio tag distributions 2002 – 2004. Year Blind Creek Big Salmon Teslin Total 2002 1.8 9.2 19.8 30.8 2003 2004 Mean SD 1.4 2.2 1.8 0.3 15.1 10.0 11.4 2.6 18.1 20.8 19.6 1.1 34.6 33.0 32.8 1.6 Data source: Osborne et al. 2003, Mercer and Eiler 2004, Mercer 2005. B. Mercer & Associates Ltd. Doc. 7-12 Page 30
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